MS001064.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS001064.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	GrpE protein homolog
Location	scaffold36: 1219164 .. 1221437 (-)
Sequence length	960
RNA-Seq Expression	MS001064.1
Synteny	MS001064.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCTTTTGCTCTCTCTAATTACTCCCTCTTCCCTCCTCGCCCTTCTACGTCATTCTCCTTCATTTCTTCTTCCTCCAAAACCCTAGAATCCACATCCCCGATTCAGCAACCTCTCTGTCTTCTTCATAATCCTCGCCGTAGCCCTCGTCTCTGTAAACCATTATTAAGTTTCTCTCCCTCCTGTTCTTCTGCAGTCAGACGTTCATTCATATCGTGTCTTTCTGCTCAAGACTCTGTTGCCAATGTACGTTAAACTTTTATCTGTTGCTTCCGTTGCTTACTGCTCTTGTTTTTATCACTTTTTAGTGCTTACTTTTTTTTTTTCCTTTCTGTCACATCGATTATTTGCCGTATTGTATTTGAATTGCTGAATGTTTCGCCAATATTGTGTCCTGATTCTTAAATGCATCGTTTGAACTTCAATTTCTATTTCTCTGTGTTGGATGGAGAGGTCTGATGCTCTGACTACAATCTGGATTTTGAGGTAAAAGCAAGAGTTTTGCATTTTACCAAGAAAGAATAAGCTGGAGGGCTAAGTTGGTTATGGTGTCAGTTTTCAATATTCGTTATATTTGTAGACGATGGCCACCTACCTAGGATTTAATATCATATGTGTTTCATTGAAATTTGTTGAGGGGCGCGAAAGTTAGTCTGGACACTCACTGATATTAAAAAAAAAATTGGTACGTTTCTTTGGCGACAATGTTGGCTGAATACTCACCCATAAGATTTAATGTTTCCATTTTTCTTGTACTTTGTAAGTGAAATGAGAGAGCAAGTGTAGCTTGGTATAATTATTTGAAAAAAGCGTATCTCAAGAATCAAGAAAGAAATGGAGAAATGGAAATAGTAAGTGTCTGTGGAAGTTTTTGGAGGATTCTCATTACTTTATTCCATTCATGGTATGAATAGCCTTCATTTTATTGTTAGGCTTATAAGATACAACTCAATGAATAAAGGACTCAGCACTCTGATCTAATTGAAAATTTTGTGTGTATTGGATTCTTCATTTTATGATGTACTTAAAGATCTTTTTTAGATACTCGATTAATTGCTGGTTGACGAAGTTAGCTACTTTATGTTTTTCTTTATTCGCATATTCAATGTAGTTGTTGGTTCCCCCTCATATAAAAAAAGGTACATAAAGAAGAGGATGATGGAAAAGCTGTGGAAAAGGCAGGAGATCAGCTTCATGAATCTAGTCTGCAGACCCTCATTAATGTATACAGAGAAGCTTTTCTTGATGGAGACCAAAAGACTGTTTCTGAAGTTGAGGCTAGACTAAAAATAATAGAGAGGGAGAAGAATGGATTGTTCCAGAAAGTGTCCAGTATATCTGCGGAGATAACGTCTGGAAAAGAGAAATATATACGCTTACAAGCTGATTTTGATAACTTCAGGAAAAGATCAGAAAAAGAAAGACATACCGTGAAGAATAATGCCCAAAAGCAAGTTATTGAGAGTCTATTACCAATGATTGACAATTTTGAGAAAGCCAGGCAACAAATTATTCCTCAAACTGACAAGGAGAAGAAGATTGATGTAAGCTACCAGGGTATATACAAACAATTCGTGGAGACCTTGAGGAGCTGGCAGGTCTCCTCTGTAGCCACTGTGGGAAGGCCATTTGATCCCTCTGTAAGTTAAATCTTTTTGACTAAAATGGGATTCAGTTCAATGTTCGAAAGTTTACTCTTTGATGCATATGTTTCTTGCTCACTAATTTATCTTACTGCTACTGGGAGGATTAAAACAGAAAAAGTATTTATTTTCCCATTGTTTATACTTAGAGTTCTGCACAAATAATTCACTTCAACTGTATCAGAACTCAGAACCACCTTAAATTGATCTAGTTGTGAAAGTAAAGGATTTACTATTGAGAGGGAATGGGTTGTAATCATGGCGACTAGCTATCTAAGATTAAATACTCAAGACTCAAGAGTGCATTAGTGGGTGGAAAATGATAATGTTTATTATCTTTACTGATGGCTATGGTAGGATGTAGATGCGAATATCCTTCACTTCTTTGTATGAGGGTAAAATATTTCTTTGGTTGTAGTTACATGAAGCCATAGCACGAGAGGAGTCATTGGAGATCAAGGAAGGAATCATCATTCAAGAATTACAACGCGGGTTTCTGCTTGGAGAGCGACTTCTAAGACCAGCAAGAGTAAAAGTATCCAGAGGTCCTGGTAGGAAGAATTCCCCCACAATCAACTCCGAGAAACCGACAGAGCAACGTGCAACTGCTGCTCAAGTAGACGAACAC

mRNA sequence

ATGGCAGCTTTTGCTCTCTCTAATTACTCCCTCTTCCCTCCTCGCCCTTCTACGTCATTCTCCTTCATTTCTTCTTCCTCCAAAACCCTAGAATCCACATCCCCGATTCAGCAACCTCTCTGTCTTCTTCATAATCCTCGCCGTAGCCCTCGTCTCTGTAAACCATTATTAAGTTTCTCTCCCTCCTGTTCTTCTGCAGTCAGACGTTCATTCATATCGTGTCTTTCTGCTCAAGACTCTGTTGCCAATGTACATAAAGAAGAGGATGATGGAAAAGCTGTGGAAAAGGCAGGAGATCAGCTTCATGAATCTAGTCTGCAGACCCTCATTAATGTATACAGAGAAGCTTTTCTTGATGGAGACCAAAAGACTGTTTCTGAAGTTGAGGCTAGACTAAAAATAATAGAGAGGGAGAAGAATGGATTGTTCCAGAAAGTGTCCAGTATATCTGCGGAGATAACGTCTGGAAAAGAGAAATATATACGCTTACAAGCTGATTTTGATAACTTCAGGAAAAGATCAGAAAAAGAAAGACATACCGTGAAGAATAATGCCCAAAAGCAAGTTATTGAGAGTCTATTACCAATGATTGACAATTTTGAGAAAGCCAGGCAACAAATTATTCCTCAAACTGACAAGGAGAAGAAGATTGATGTAAGCTACCAGGGTATATACAAACAATTCGTGGAGACCTTGAGGAGCTGGCAGGTCTCCTCTGTAGCCACTGTGGGAAGGCCATTTGATCCCTCTTTACATGAAGCCATAGCACGAGAGGAGTCATTGGAGATCAAGGAAGGAATCATCATTCAAGAATTACAACGCGGGTTTCTGCTTGGAGAGCGACTTCTAAGACCAGCAAGAGTAAAAGTATCCAGAGGTCCTGGTAGGAAGAATTCCCCCACAATCAACTCCGAGAAACCGACAGAGCAACGTGCAACTGCTGCTCAAGTAGACGAACAC

Coding sequence (CDS)

ATGGCAGCTTTTGCTCTCTCTAATTACTCCCTCTTCCCTCCTCGCCCTTCTACGTCATTCTCCTTCATTTCTTCTTCCTCCAAAACCCTAGAATCCACATCCCCGATTCAGCAACCTCTCTGTCTTCTTCATAATCCTCGCCGTAGCCCTCGTCTCTGTAAACCATTATTAAGTTTCTCTCCCTCCTGTTCTTCTGCAGTCAGACGTTCATTCATATCGTGTCTTTCTGCTCAAGACTCTGTTGCCAATGTACATAAAGAAGAGGATGATGGAAAAGCTGTGGAAAAGGCAGGAGATCAGCTTCATGAATCTAGTCTGCAGACCCTCATTAATGTATACAGAGAAGCTTTTCTTGATGGAGACCAAAAGACTGTTTCTGAAGTTGAGGCTAGACTAAAAATAATAGAGAGGGAGAAGAATGGATTGTTCCAGAAAGTGTCCAGTATATCTGCGGAGATAACGTCTGGAAAAGAGAAATATATACGCTTACAAGCTGATTTTGATAACTTCAGGAAAAGATCAGAAAAAGAAAGACATACCGTGAAGAATAATGCCCAAAAGCAAGTTATTGAGAGTCTATTACCAATGATTGACAATTTTGAGAAAGCCAGGCAACAAATTATTCCTCAAACTGACAAGGAGAAGAAGATTGATGTAAGCTACCAGGGTATATACAAACAATTCGTGGAGACCTTGAGGAGCTGGCAGGTCTCCTCTGTAGCCACTGTGGGAAGGCCATTTGATCCCTCTTTACATGAAGCCATAGCACGAGAGGAGTCATTGGAGATCAAGGAAGGAATCATCATTCAAGAATTACAACGCGGGTTTCTGCTTGGAGAGCGACTTCTAAGACCAGCAAGAGTAAAAGTATCCAGAGGTCCTGGTAGGAAGAATTCCCCCACAATCAACTCCGAGAAACCGACAGAGCAACGTGCAACTGCTGCTCAAGTAGACGAACAC

Protein sequence

MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFSPSCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSPTINSEKPTEQRATAAQVDEH

Homology

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BLAST of MS001064.1 vs. NCBI nr
Match: XP_022131274.1 (uncharacterized protein LOC111004544 [Momordica charantia])

HSP 1 Score: 603.2 bits (1554), Expect = 1.3e-168
Identity = 318/320 (99.38%), Postives = 319/320 (99.69%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAAFALSNYSLF PRPSTSFSFISSSS+TLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS
Sbjct: 1   MAAFALSNYSLFSPRPSTSFSFISSSSETLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60

Query: 61  PSCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDG 120
           PSCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDG
Sbjct: 61  PSCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDG 120

Query: 121 DQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHT 180
           DQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHT
Sbjct: 121 DQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHT 180

Query: 181 VKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSV 240
           VKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSV
Sbjct: 181 VKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSV 240

Query: 241 ATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSP 300
           ATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSP
Sbjct: 241 ATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSP 300

Query: 301 TINSEKPTEQRATAAQVDEH 321
           TINSEKPTEQRATAAQVDEH
Sbjct: 301 TINSEKPTEQRATAAQVDEH 320

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BLAST of MS001064.1 vs. NCBI nr
Match: XP_038884130.1 (protein GrpE [Benincasa hispida])

HSP 1 Score: 479.9 bits (1234), Expect = 1.7e-131
Identity = 261/322 (81.06%), Postives = 286/322 (88.82%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAAFALSNY+L  PRPST    +SSSSKTLESTS I QPLCL HNP    RLC  LLSFS
Sbjct: 1   MAAFALSNYTLISPRPST--PLVSSSSKTLESTSLIHQPLCLRHNPPHRSRLCNSLLSFS 60

Query: 61  PSC-SSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120
           P+  SS V RSF+SCLSA DSVANV+ EEDDGKA+EK G Q    SLQTLI+VYREAFLD
Sbjct: 61  PTTPSSPVTRSFVSCLSAHDSVANVNNEEDDGKAMEKDGYQFDRYSLQTLIDVYREAFLD 120

Query: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180
           GDQKTVSEVEA++KII REK+GLFQKVS++SAEITSGKEKYIRLQADFDNFRKRSEKE+H
Sbjct: 121 GDQKTVSEVEAKIKIIGREKDGLFQKVSNVSAEITSGKEKYIRLQADFDNFRKRSEKEQH 180

Query: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240
           TVKNNAQK+VIESLLP+IDNFEKARQQI+PQTDKEKKID+SYQGIYKQFVETLRSW+VS+
Sbjct: 181 TVKNNAQKEVIESLLPIIDNFEKARQQIVPQTDKEKKIDISYQGIYKQFVETLRSWRVSA 240

Query: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           VATVGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPGR+NS
Sbjct: 241 VATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRRNS 300

Query: 301 PTINS-EKPTEQRATAAQVDEH 321
           PTIN+ +KP    A AA VDEH
Sbjct: 301 PTINAGQKP----AAAAGVDEH 316

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BLAST of MS001064.1 vs. NCBI nr
Match: KAG6585280.1 (hypothetical protein SDJN03_18013, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 471.9 bits (1213), Expect = 4.5e-129
Identity = 255/322 (79.19%), Postives = 279/322 (86.65%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAA ALSNY+LF PRPST       SSK +ESTS +   LC LHNPR   R C PLLSFS
Sbjct: 1   MAALALSNYTLFSPRPST-------SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFS 60

Query: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120
           P S SS VRRSF+ C SAQDSV NV+KEEDDG+   KAGD+LH  SLQ LINVYREAFLD
Sbjct: 61  PASQSSTVRRSFLPCFSAQDSVPNVNKEEDDGEDGSKAGDELHGLSLQALINVYREAFLD 120

Query: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180
           GDQKT+S+VEA+LKI EREK+GLFQKVS+ SAEITSGKE YIRLQADFDNFRKRSEKER 
Sbjct: 121 GDQKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERL 180

Query: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240
           TVKNNAQK+VIE+LLPMID+FEKARQQI+PQTDKEKKIDVSYQGIYKQFVE LRSW++S+
Sbjct: 181 TVKNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISA 240

Query: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           VA VGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPG+KNS
Sbjct: 241 VAAVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNS 300

Query: 301 PTINSEKPTEQRATA-AQVDEH 321
           PT NSEKPTE  ATA A+VDEH
Sbjct: 301 PTTNSEKPTEHPATATARVDEH 315

[Close]

BLAST of MS001064.1 vs. NCBI nr
Match: KAG7020199.1 (grpE [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 471.9 bits (1213), Expect = 4.5e-129
Identity = 255/322 (79.19%), Postives = 279/322 (86.65%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAA ALSNY+LF PRPST       SSK +ESTS +   LC LHNPR   R C PLLSFS
Sbjct: 1   MAALALSNYTLFSPRPST-------SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFS 60

Query: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120
           P S SS VRRSF+ C SAQDSV NV+KEEDDG+   KAGD+LH  SLQ LINVYREAFLD
Sbjct: 61  PASHSSTVRRSFLPCFSAQDSVPNVNKEEDDGEDGSKAGDELHGLSLQALINVYREAFLD 120

Query: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180
           GDQKT+S+VEA+LKI EREK+GLFQKVS+ SAEITSGKE YIRLQADFDNFRKRSEKER 
Sbjct: 121 GDQKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERL 180

Query: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240
           TVKNNAQK+VIE+LLPMID+FEKARQQI+PQTDKEKKIDVSYQGIYKQFVE LRSW++S+
Sbjct: 181 TVKNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISA 240

Query: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           VA VGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPG+KNS
Sbjct: 241 VAAVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNS 300

Query: 301 PTINSEKPTEQRATA-AQVDEH 321
           PT NSEKPTE  ATA A+VDEH
Sbjct: 301 PTTNSEKPTEHPATATARVDEH 315

[Close]

BLAST of MS001064.1 vs. NCBI nr
Match: XP_022951572.1 (uncharacterized protein LOC111454347 isoform X2 [Cucurbita moschata])

HSP 1 Score: 471.1 bits (1211), Expect = 7.7e-129
Identity = 254/322 (78.88%), Postives = 279/322 (86.65%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAA ALSNY+LF PRPST       SSK +ESTS +   LC LHNPR   R C PLLSFS
Sbjct: 1   MAALALSNYTLFSPRPST-------SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFS 60

Query: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120
           P S SS VRRSF+ C SAQDSV NV+KEEDDG+   KAGD+LH  SLQ LIN+YREAFLD
Sbjct: 61  PASHSSPVRRSFLPCFSAQDSVPNVNKEEDDGEDGNKAGDELHGLSLQALINIYREAFLD 120

Query: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180
           GDQKT+S+VEA+LKI EREK+GLFQKVS+ SAEITSGKE YIRLQADFDNFRKRSEKER 
Sbjct: 121 GDQKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERL 180

Query: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240
           TVKNNAQK+VIE+LLPMID+FEKARQQI+PQTDKEKKIDVSYQGIYKQFVE LRSW++S+
Sbjct: 181 TVKNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISA 240

Query: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           VA VGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPG+KNS
Sbjct: 241 VAAVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNS 300

Query: 301 PTINSEKPTEQRATA-AQVDEH 321
           PT NSEKPTE  ATA A+VDEH
Sbjct: 301 PTTNSEKPTEHPATATARVDEH 315

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BLAST of MS001064.1 vs. ExPASy Swiss-Prot
Match: Q7V3Q4 (Protein GrpE OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / NIES-2087 / MED4) OX=59919 GN=grpE PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.3e-34
Identity = 87/241 (36.10%), Postives = 140/241 (58.09%), Query Frame = 0


Query: 78  QDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQKTVSEVEARLKIIER 137
           +D+V+    + +D   +E    +  ++      N+Y E      + T++  +ARL+ +E+
Sbjct: 13  EDNVSQEVNKNEDSSVIENQTSEDKQTLEVDDENIYAEDL----KNTITNNDARLEQLEK 72

Query: 138 EKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLLPMI 197
           E   L              K +Y+R+ ADFDNFRKR  +++  +K     + + ++LP++
Sbjct: 73  EHETL--------------KSQYVRIAADFDNFRKRQSRDQDDLKVQLVSKALTAILPIV 132

Query: 198 DNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEAIAR 257
           DNFE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS +  V + FDP LHEA+ R
Sbjct: 133 DNFERARQQLKPESEEAQTLHRSYQGLYKQLVEVLKQQGVSPMRVVAQQFDPKLHEAVLR 192

Query: 258 EESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSPTINSEKPTEQRATAAQV 317
           E S E  E III+ELQRG+ L  ++LR A VKVS GPG++     NS++P E+      +
Sbjct: 193 EPSQEFNEDIIIEELQRGYHLEGKVLRHALVKVSMGPGQQ-----NSQEPEEKDKVEEDI 230

Query: 318 D 319
           D
Sbjct: 253 D 230

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BLAST of MS001064.1 vs. ExPASy Swiss-Prot
Match: A8G203 (Protein GrpE OS=Prochlorococcus marinus (strain MIT 9215) OX=93060 GN=grpE PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.4e-34
Identity = 92/243 (37.86%), Postives = 143/243 (58.85%), Query Frame = 0


Query: 79  DSVANVHKE--EDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQK-TVSEVEARLKII 138
           D++ N  K+    D    + + +Q   +    L +  +EA    + K T+S  +ARLK +
Sbjct: 7   DNIDNKEKDVSNQDNAPEDISSEQNSTNENDELTSQKKEAINTEELKNTISNNDARLKQL 66

Query: 139 EREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLLP 198
           E+E   L              K +Y+R+ ADFDNFRKR  +++  +K     + + ++LP
Sbjct: 67  EKEHETL--------------KNQYVRISADFDNFRKRQSRDQDDLKIQIVSKTLTAILP 126

Query: 199 MIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEAI 258
           ++DNFE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS +  VG+ FDP+LHEA+
Sbjct: 127 IVDNFERARQQLQPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAV 186

Query: 259 AREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSPTINSEKPTEQRATAA 318
            RE S E  E  II+ELQRG+ L  ++LR A VKVS GPG++NS     EK T +    +
Sbjct: 187 LREPSEESDEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQQ-EVEKDTVEEDVNS 234

[Close]

BLAST of MS001064.1 vs. ExPASy Swiss-Prot
Match: Q31DG8 (Protein GrpE OS=Prochlorococcus marinus (strain MIT 9312) OX=74546 GN=grpE PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.4e-34
Identity = 85/212 (40.09%), Postives = 130/212 (61.32%), Query Frame = 0


Query: 88  EDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQKTVSEVEARLKIIEREKNGLFQKVS 147
           ED+  A EK  +    S  +T      E   +  + T+S  +ARL+ +E+E   L     
Sbjct: 24  EDNSSAAEKTIENDELSPQKT-----EEINTEELKNTISNNDARLEQLEKEHETL----- 83

Query: 148 SISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLLPMIDNFEKARQQI 207
                    K +Y+R+ ADFDNFRKR  +++  +K     + + ++LP++DNFE+ARQQ+
Sbjct: 84  ---------KNQYVRISADFDNFRKRQSRDQDDLKVQLVSKTLTAILPIVDNFERARQQL 143

Query: 208 IPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEAIAREESLEIKEGI 267
            P++++ + +  SYQG+YKQ VE L+   VS +  VG+ FDP+LHEA+ RE S E KE +
Sbjct: 144 KPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAVLREPSEEFKEDL 203

Query: 268 IIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           I++ELQRG+ L  ++LR A VKVS G G++NS
Sbjct: 204 IVEELQRGYHLEGKVLRHALVKVSMGHGQQNS 216

[Close]

BLAST of MS001064.1 vs. ExPASy Swiss-Prot
Match: A2BNE2 (Protein GrpE OS=Prochlorococcus marinus (strain AS9601) OX=146891 GN=grpE PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.4e-34
Identity = 77/178 (43.26%), Postives = 118/178 (66.29%), Query Frame = 0


Query: 122 QKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTV 181
           + ++S  +ARL+ +E+E   L              K +Y+R+ ADFDNFRKR  +++  +
Sbjct: 53  KNSISNNDARLEQLEKEHETL--------------KNQYVRISADFDNFRKRQSRDQDDL 112

Query: 182 KNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVA 241
           K     + + ++LP++DNFE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS + 
Sbjct: 113 KIQLVSKTLTAILPIVDNFERARQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMR 172

Query: 242 TVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
            VG+ FDPSLHEA+ RE S + +E  II+ELQRG+ L  ++LR A VKVS GPG++NS
Sbjct: 173 VVGQQFDPSLHEAVLREPSEKFEEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNS 216

[Close]

BLAST of MS001064.1 vs. ExPASy Swiss-Prot
Match: A3PA63 (Protein GrpE OS=Prochlorococcus marinus (strain MIT 9301) OX=167546 GN=grpE PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.2e-33
Identity = 76/178 (42.70%), Postives = 116/178 (65.17%), Query Frame = 0


Query: 122 QKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTV 181
           + T+S  +ARL+ +E+E   L              K +Y+R+ ADFDNFRKR  +++  +
Sbjct: 53  KNTISNNDARLEQLEKEHETL--------------KNQYVRISADFDNFRKRQSRDQDDL 112

Query: 182 KNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVA 241
           K     + + ++LP++DNFE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS + 
Sbjct: 113 KIQLVSKTLTAILPIVDNFERARQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMR 172

Query: 242 TVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
            VG+ FDP+LHEA+ RE S E +E  II+ELQRG+ L  ++LR A  KVS GPG++ S
Sbjct: 173 VVGQQFDPNLHEAVLREPSEEFEEDFIIEELQRGYHLEGKVLRHALAKVSMGPGKQKS 216

[Close]

BLAST of MS001064.1 vs. ExPASy TrEMBL
Match: A0A6J1BPS6 (GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111004544 PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 6.3e-169
Identity = 318/320 (99.38%), Postives = 319/320 (99.69%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAAFALSNYSLF PRPSTSFSFISSSS+TLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS
Sbjct: 1   MAAFALSNYSLFSPRPSTSFSFISSSSETLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60

Query: 61  PSCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDG 120
           PSCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDG
Sbjct: 61  PSCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDG 120

Query: 121 DQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHT 180
           DQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHT
Sbjct: 121 DQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHT 180

Query: 181 VKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSV 240
           VKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSV
Sbjct: 181 VKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSV 240

Query: 241 ATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSP 300
           ATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSP
Sbjct: 241 ATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSP 300

Query: 301 TINSEKPTEQRATAAQVDEH 321
           TINSEKPTEQRATAAQVDEH
Sbjct: 301 TINSEKPTEQRATAAQVDEH 320

[Close]

BLAST of MS001064.1 vs. ExPASy TrEMBL
Match: A0A6J1GI26 (GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.7e-129
Identity = 254/322 (78.88%), Postives = 279/322 (86.65%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAA ALSNY+LF PRPST       SSK +ESTS +   LC LHNPR   R C PLLSFS
Sbjct: 1   MAALALSNYTLFSPRPST-------SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFS 60

Query: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120
           P S SS VRRSF+ C SAQDSV NV+KEEDDG+   KAGD+LH  SLQ LIN+YREAFLD
Sbjct: 61  PASHSSPVRRSFLPCFSAQDSVPNVNKEEDDGEDGNKAGDELHGLSLQALINIYREAFLD 120

Query: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180
           GDQKT+S+VEA+LKI EREK+GLFQKVS+ SAEITSGKE YIRLQADFDNFRKRSEKER 
Sbjct: 121 GDQKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERL 180

Query: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240
           TVKNNAQK+VIE+LLPMID+FEKARQQI+PQTDKEKKIDVSYQGIYKQFVE LRSW++S+
Sbjct: 181 TVKNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISA 240

Query: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           VA VGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPG+KNS
Sbjct: 241 VAAVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNS 300

Query: 301 PTINSEKPTEQRATA-AQVDEH 321
           PT NSEKPTE  ATA A+VDEH
Sbjct: 301 PTTNSEKPTEHPATATARVDEH 315

[Close]

BLAST of MS001064.1 vs. ExPASy TrEMBL
Match: A0A6J1GHY1 (GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 9.2e-128
Identity = 254/323 (78.64%), Postives = 279/323 (86.38%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAA ALSNY+LF PRPST       SSK +ESTS +   LC LHNPR   R C PLLSFS
Sbjct: 1   MAALALSNYTLFSPRPST-------SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFS 60

Query: 61  P-SCSSAVRRSFISCLSAQDSVAN-VHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFL 120
           P S SS VRRSF+ C SAQDSV N V+KEEDDG+   KAGD+LH  SLQ LIN+YREAFL
Sbjct: 61  PASHSSPVRRSFLPCFSAQDSVPNKVNKEEDDGEDGNKAGDELHGLSLQALINIYREAFL 120

Query: 121 DGDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKER 180
           DGDQKT+S+VEA+LKI EREK+GLFQKVS+ SAEITSGKE YIRLQADFDNFRKRSEKER
Sbjct: 121 DGDQKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKER 180

Query: 181 HTVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVS 240
            TVKNNAQK+VIE+LLPMID+FEKARQQI+PQTDKEKKIDVSYQGIYKQFVE LRSW++S
Sbjct: 181 LTVKNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRIS 240

Query: 241 SVATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKN 300
           +VA VGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPG+KN
Sbjct: 241 AVAAVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKN 300

Query: 301 SPTINSEKPTEQRATA-AQVDEH 321
           SPT NSEKPTE  ATA A+VDEH
Sbjct: 301 SPTTNSEKPTEHPATATARVDEH 316

[Close]

BLAST of MS001064.1 vs. ExPASy TrEMBL
Match: A0A5A7VFZ8 (GrpE protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001230 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.3e-126
Identity = 251/321 (78.19%), Postives = 280/321 (87.23%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAAFALSNY+LF PRPS+   F+SSSSKTL+STSP  QPL LLHNPR   RLC PL +FS
Sbjct: 1   MAAFALSNYTLFSPRPSS--PFLSSSSKTLDSTSPFHQPLPLLHNPRHRSRLCDPLFNFS 60

Query: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120
           P S SS + RSF  CLSA  SVANV+ EEDDGKAVEK G +   SSLQTLI VYREAFLD
Sbjct: 61  PTSSSSPLTRSFAPCLSAHSSVANVNNEEDDGKAVEKDGYKFDGSSLQTLIEVYREAFLD 120

Query: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180
           GDQKTVSEVEAR+KII REK+ L +K+S+IS E+TSGKEKYIRLQADFDNFRKRSEKE+H
Sbjct: 121 GDQKTVSEVEARIKIIGREKDELSRKLSNISTEMTSGKEKYIRLQADFDNFRKRSEKEQH 180

Query: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240
            VKNNAQK+VIESLLPMID+FEKARQQI+PQTDKEKKID+SYQGIYKQFVETLRSW+VS+
Sbjct: 181 NVKNNAQKEVIESLLPMIDHFEKARQQIVPQTDKEKKIDISYQGIYKQFVETLRSWRVSA 240

Query: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           VATVGRPFDPSLHEA+AREES EIKEGI+IQEL+RGFLLGERLLRPARVKVS+GPGRK+S
Sbjct: 241 VATVGRPFDPSLHEAVAREESQEIKEGIVIQELRRGFLLGERLLRPARVKVSKGPGRKSS 300

Query: 301 PTINSEKPTEQRATAAQVDEH 321
            TI+ +   +Q A AA VDEH
Sbjct: 301 RTIDGK---QQPAAAAGVDEH 316

[Close]

BLAST of MS001064.1 vs. ExPASy TrEMBL
Match: A0A1S3BDD3 (GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103488418 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 5.0e-126
Identity = 251/321 (78.19%), Postives = 279/321 (86.92%), Query Frame = 0


Query: 1   MAAFALSNYSLFPPRPSTSFSFISSSSKTLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60
           MAAFALSNY+LF PRPS+   F+SSSSKTL+STSP  QPL LLHNPR   RLC PL SFS
Sbjct: 1   MAAFALSNYTLFSPRPSS--PFLSSSSKTLDSTSPFHQPLPLLHNPRHRSRLCDPLFSFS 60

Query: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120
           P S SS + RSF  CLSA  SVANV+ EEDDGKAVEK G +   SSLQTLI VYREAFLD
Sbjct: 61  PTSSSSPLTRSFAPCLSAHSSVANVNNEEDDGKAVEKDGYKFDGSSLQTLIEVYREAFLD 120

Query: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180
           GDQKTVSEVEAR+KII REK+ L +K+S+I  E+TSGKEKYIRLQADFDNFRKRSEKE+H
Sbjct: 121 GDQKTVSEVEARIKIIGREKDELSRKLSNILTEMTSGKEKYIRLQADFDNFRKRSEKEQH 180

Query: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240
            VKNNAQK+VIESLLPMID+FEKARQQI+PQTDKEKKID+SYQGIYKQFVETLRSW+VS+
Sbjct: 181 NVKNNAQKEVIESLLPMIDHFEKARQQIVPQTDKEKKIDISYQGIYKQFVETLRSWRVSA 240

Query: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300
           VATVGRPFDPSLHEA+AREES EIKEGI+IQEL+RGFLLGERLLRPARVKVS+GPGRK+S
Sbjct: 241 VATVGRPFDPSLHEAVAREESQEIKEGIVIQELRRGFLLGERLLRPARVKVSKGPGRKSS 300

Query: 301 PTINSEKPTEQRATAAQVDEH 321
            TI+ +   +Q A AA VDEH
Sbjct: 301 RTIDGK---QQPAAAAGVDEH 316

[Close]

BLAST of MS001064.1 vs. TAIR 10
Match: AT1G36390.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 225.3 bits (573), Expect = 6.8e-59
Identity = 137/291 (47.08%), Postives = 188/291 (64.60%), Query Frame = 0


Query: 16  PSTSFSFISSSSKTLESTSPIQQPLCLLHNPRR-SPRLCKPLLSFSPSCSSAVRRSFISC 75
           P  SFS  SSSSKTL    P++  L     P   SP + KP   F               
Sbjct: 13  PCFSFSSPSSSSKTL----PLRNNLHFHGRPSPISPLISKPSRKF--------------- 72

Query: 76  LSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQKTVSEVEARLKI 135
                 + + H+  +  +A  K      ++ ++TLI  Y++A L+GD+ +V+E+E     
Sbjct: 73  -----PIFSAHQTNNSEEANSK-----QQADVKTLIRSYKQALLNGDETSVTEIETMFCK 132

Query: 136 IEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLL 195
           IE+EKN + QKV S+S +I S KE  IRLQADFDN RK+ +K+R + ++NA+ Q+++SLL
Sbjct: 133 IEKEKNKMDQKVLSLSMKIASEKEMKIRLQADFDNTRKKLDKDRLSTESNAKVQILKSLL 192

Query: 196 PMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEA 255
           P+ID+FEKA+ Q+   TDKEKKID SYQGIY+QFVE LR  +VS +ATVG+PFDP LHEA
Sbjct: 193 PIIDSFEKAKLQVRVDTDKEKKIDTSYQGIYRQFVEVLRYLRVSVIATVGKPFDPLLHEA 252

Query: 256 IAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSPTINSE 306
           I+REES  +K GII +EL +GF+LG+R+LRPA+VKVS GP  K +P+   E
Sbjct: 253 ISREESEAVKAGIITEELNKGFVLGDRVLRPAKVKVSLGPVNKKTPSAAEE 274

[Close]

BLAST of MS001064.1 vs. TAIR 10
Match: AT1G36390.2 (Co-chaperone GrpE family protein )

HSP 1 Score: 225.3 bits (573), Expect = 6.8e-59
Identity = 137/291 (47.08%), Postives = 188/291 (64.60%), Query Frame = 0


Query: 16  PSTSFSFISSSSKTLESTSPIQQPLCLLHNPRR-SPRLCKPLLSFSPSCSSAVRRSFISC 75
           P  SFS  SSSSKTL    P++  L     P   SP + KP   F               
Sbjct: 13  PCFSFSSPSSSSKTL----PLRNNLHFHGRPSPISPLISKPSRKF--------------- 72

Query: 76  LSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQKTVSEVEARLKI 135
                 + + H+  +  +A  K      ++ ++TLI  Y++A L+GD+ +V+E+E     
Sbjct: 73  -----PIFSAHQTNNSEEANSK-----QQADVKTLIRSYKQALLNGDETSVTEIETMFCK 132

Query: 136 IEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLL 195
           IE+EKN + QKV S+S +I S KE  IRLQADFDN RK+ +K+R + ++NA+ Q+++SLL
Sbjct: 133 IEKEKNKMDQKVLSLSMKIASEKEMKIRLQADFDNTRKKLDKDRLSTESNAKVQILKSLL 192

Query: 196 PMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEA 255
           P+ID+FEKA+ Q+   TDKEKKID SYQGIY+QFVE LR  +VS +ATVG+PFDP LHEA
Sbjct: 193 PIIDSFEKAKLQVRVDTDKEKKIDTSYQGIYRQFVEVLRYLRVSVIATVGKPFDPLLHEA 252

Query: 256 IAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSPTINSE 306
           I+REES  +K GII +EL +GF+LG+R+LRPA+VKVS GP  K +P+   E
Sbjct: 253 ISREESEAVKAGIITEELNKGFVLGDRVLRPAKVKVSLGPVNKKTPSAAEE 274

[Close]

BLAST of MS001064.1 vs. TAIR 10
Match: AT5G17710.2 (Co-chaperone GrpE family protein )

HSP 1 Score: 202.6 bits (514), Expect = 4.8e-52
Identity = 110/233 (47.21%), Postives = 159/233 (68.24%), Query Frame = 0


Query: 78  QDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQKTVSEVEARLKIIER 137
           Q++   V  E ++  A E   ++   + +  L+  Y+EA  D ++  ++E+EA LK IE 
Sbjct: 84  QETDGAVDVENENASAEEGEAEEEEAAVITALLKSYKEALADNNEGKIAEIEASLKSIED 143

Query: 138 EKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLLPMI 197
           EK  L  KV+S+S E++  +++ IR+ ADFDNFRKR+E+ER  + +NAQ +V+E+LL ++
Sbjct: 144 EKFLLADKVASLSNELSVERDRLIRISADFDNFRKRTERERLNLVSNAQGEVVENLLAVL 203

Query: 198 DNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEAIAR 257
           DNFE+A+ QI  +T+ E+K+  SYQ IYKQFVE L S  V  V TVG+ FDP LHEAI R
Sbjct: 204 DNFERAKSQIKVETEGEEKVTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMR 263

Query: 258 EESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSPTINSEKPTEQ 311
           E+S E +EGI+++E ++GFLLGERLLRP+ VKVS GPG +       E+ T Q
Sbjct: 264 EDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPEKPLEAEGEEATAQ 316

[Close]

BLAST of MS001064.1 vs. TAIR 10
Match: AT5G17710.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 201.8 bits (512), Expect = 8.1e-52
Identity = 109/227 (48.02%), Postives = 156/227 (68.72%), Query Frame = 0


Query: 84  VHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLDGDQKTVSEVEARLKIIEREKNGLF 143
           V  E ++  A E   ++   + +  L+  Y+EA  D ++  ++E+EA LK IE EK  L 
Sbjct: 88  VDVENENASAEEGEAEEEEAAVITALLKSYKEALADNNEGKIAEIEASLKSIEDEKFLLA 147

Query: 144 QKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERHTVKNNAQKQVIESLLPMIDNFEKA 203
            KV+S+S E++  +++ IR+ ADFDNFRKR+E+ER  + +NAQ +V+E+LL ++DNFE+A
Sbjct: 148 DKVASLSNELSVERDRLIRISADFDNFRKRTERERLNLVSNAQGEVVENLLAVLDNFERA 207

Query: 204 RQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSSVATVGRPFDPSLHEAIAREESLEI 263
           + QI  +T+ E+K+  SYQ IYKQFVE L S  V  V TVG+ FDP LHEAI RE+S E 
Sbjct: 208 KSQIKVETEGEEKVTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMREDSAEY 267

Query: 264 KEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNSPTINSEKPTEQ 311
           +EGI+++E ++GFLLGERLLRP+ VKVS GPG +       E+ T Q
Sbjct: 268 EEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPEKPLEAEGEEATAQ 314

[Close]

BLAST of MS001064.1 vs. TAIR 10
Match: AT4G26780.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.0e-09
Identity = 46/191 (24.08%), Postives = 87/191 (45.55%), Query Frame = 0


Query: 119 DGDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKER 178
           D D+ +  ++   +K++  ++  L +K      EI   K+K +R  A+ +N   R+ ++ 
Sbjct: 135 DDDELSADDL---VKLVAEKEELLSEK----EEEIKQLKDKVLRTYAEMENVMDRTRRDA 194

Query: 179 HTVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVS-------------YQGI- 238
              K  A +   +SLL + DN  +A   +    +   K+D S              +G+ 
Sbjct: 195 ENTKKYAVQNFAKSLLDVADNLGRASSVV---KESFSKLDTSEDSAGAAPLLKTLLEGVE 254

Query: 239 --YKQFVETLRSWQVSSVATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERL 294
              KQ  E  + + +     +  PFDP+ H A+ +       EG +   L+ G+ L +R+
Sbjct: 255 MTEKQLAEVFKKFGMEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRV 314

The following BLAST results are available for this feature:

BLAST of MS001064.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131274.1	1.3e-168	99.38	uncharacterized protein LOC111004544 [Momordica charantia]	[more]
XP_038884130.1	1.7e-131	81.06	protein GrpE [Benincasa hispida]	[more]
KAG6585280.1	4.5e-129	79.19	hypothetical protein SDJN03_18013, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7020199.1	4.5e-129	79.19	grpE [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022951572.1	7.7e-129	78.88	uncharacterized protein LOC111454347 isoform X2 [Cucurbita moschata]	[more]

BLAST of MS001064.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7V3Q4	3.3e-34	36.10	Protein GrpE OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / NIES-...	[more]
A8G203	4.4e-34	37.86	Protein GrpE OS=Prochlorococcus marinus (strain MIT 9215) OX=93060 GN=grpE PE=3 ...	[more]
Q31DG8	4.4e-34	40.09	Protein GrpE OS=Prochlorococcus marinus (strain MIT 9312) OX=74546 GN=grpE PE=3 ...	[more]
A2BNE2	4.4e-34	43.26	Protein GrpE OS=Prochlorococcus marinus (strain AS9601) OX=146891 GN=grpE PE=3 S...	[more]
A3PA63	2.2e-33	42.70	Protein GrpE OS=Prochlorococcus marinus (strain MIT 9301) OX=167546 GN=grpE PE=3...	[more]

BLAST of MS001064.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BPS6	6.3e-169	99.38	GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111004544 PE=3 SV=1	[more]
A0A6J1GI26	3.7e-129	78.88	GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1	[more]
A0A6J1GHY1	9.2e-128	78.64	GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1	[more]
A0A5A7VFZ8	2.3e-126	78.19	GrpE protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G...	[more]
A0A1S3BDD3	5.0e-126	78.19	GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103488418 PE=3 SV=1	[more]

BLAST of MS001064.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36390.1	6.8e-59	47.08	Co-chaperone GrpE family protein	[more]
AT1G36390.2	6.8e-59	47.08	Co-chaperone GrpE family protein	[more]
AT5G17710.2	4.8e-52	47.21	Co-chaperone GrpE family protein	[more]
AT5G17710.1	8.1e-52	48.02	Co-chaperone GrpE family protein	[more]
AT4G26780.1	5.0e-09	24.08	Co-chaperone GrpE family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 118..145
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 289..320
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 296..311
None	No IPR available	PANTHER	PTHR21237:SF4	GRPE PROTEIN HOMOLOG	coord: 1..306
IPR000740	GrpE nucleotide exchange factor	PRINTS	PR00773	GRPEPROTEIN	coord: 189..204 score: 37.3 coord: 271..290 score: 49.06 coord: 244..259 score: 48.24 coord: 161..177 score: 46.88
IPR000740	GrpE nucleotide exchange factor	PFAM	PF01025	GrpE	coord: 138..291 e-value: 5.9E-40 score: 136.7
IPR000740	GrpE nucleotide exchange factor	PANTHER	PTHR21237	GRPE PROTEIN	coord: 1..306
IPR000740	GrpE nucleotide exchange factor	PROSITE	PS01071	GRPE	coord: 247..290
IPR000740	GrpE nucleotide exchange factor	HAMAP	MF_01151	GrpE	coord: 78..292 score: 17.53516
IPR000740	GrpE nucleotide exchange factor	CDD	cd00446	GrpE	coord: 157..290 e-value: 3.26086E-46 score: 151.119
IPR013805	GrpE nucleotide exchange factor, coiled-coil	GENE3D	3.90.20.20		coord: 120..233 e-value: 7.0E-22 score: 79.3
IPR013805	GrpE nucleotide exchange factor, coiled-coil	SUPERFAMILY	58014	Coiled-coil domain of nucleotide exchange factor GrpE	coord: 143..234
IPR009012	GrpE nucleotide exchange factor, head	GENE3D	2.30.22.10	Head domain of nucleotide exchange factor GrpE	coord: 237..292 e-value: 3.6E-18 score: 67.3
IPR009012	GrpE nucleotide exchange factor, head	SUPERFAMILY	51064	Head domain of nucleotide exchange factor GrpE	coord: 239..293

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS001064	MS001064	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS001064.1-cds	MS001064.1-cds-scaffold36:1219164..1219373	CDS
MS001064.1-cds	MS001064.1-cds-scaffold36:1219795..1220295	CDS
MS001064.1-cds	MS001064.1-cds-scaffold36:1221189..1221437	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS001064.1	MS001064.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
molecular_function	GO:0000774	adenyl-nucleotide exchange factor activity
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0042803	protein homodimerization activity