MS001050.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS001050.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNA pseudouridine synthase 3, mitochondrial
Location	scaffold36: 1073888 .. 1077617 (-)
Sequence length	1422
RNA-Seq Expression	MS001050.1
Synteny	MS001050.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCAGAGCATACGGCGGCATCTTTTACTCGCAGCCAGATACTACTCCAGAATTTCTCCTCCACCACCTGCATATGCTAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCATTGCCCCCATTTCCTGGTTACCCTTTGCCTCGGAAGAATCATATCGGTATATCTGGGGGGTCAACTCATGTCACTGCCATAAATTGGGTCAAGCACTATTTTGATGAAATTCATGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTAAGTACGAACACTTGCTCTTATTTTAGCTAATTGGTTTTGTCCTTTTGTCCTTTTCATGGTATGCTTATATCTAGTCTTGTAGGCTTCATGTAACTTCTTTCATGTCGAGAATCCCCAGTAAACTTTTTGAGTTGTGAGACAAGTTATAAGTTGTAAATTAGTAGCGGGAACTTAAACTGTTCTTGTTTGTTTAAAGAAAATTGAGTTCCTTAGATAAATTTACAAGAGAAAATGTACATCGGACTTGTTTGCTTTTAGAATTTCAGTTAGTGTTTGTGTTATATCTTCGAGGAAAAACTTAAGGGCAACAACTTATCTGGATGGATATTCAAGGATATTTCACAACTTCAACATAACACATGGTTTTTATCTCTCTTTATCTCTCAAGGCAATAATAAAAGCTCGATTGATGATTATCTAATCAATTCTGAGTTTAAATTTATCTTCCCTTTTCCAGGTCCAGGTGGAATGTCCACAATCGGGTGGATCAAATGGAAAAGGAAATTTGAAACCTATGAGAAAGGTATTGTTTTTTGAATTAACGGCCAATTCTCTTTTGAAGATTCAAAATGGAATTTCAATGTGGACACGATATTTGGTTTCATTTACTTATTCAAGTCATTCTTTGTTGGTCTGTTAACTTATTACCAGATGATTTTCAGATTAAGAGTAATGAGATTATGGAAACTGGGGCAAGAGTCCATATACCTGTGTCAGTTGCGGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGTACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTTAAGTACAAGGCAAGTACCAAGTTTCACTTGCATTCTTCTCTTTGTTGTTTCCTGCTACTAATGAATCTCCTAGTATTTTACCTATTGGTTCTAGAAGCAGTTGCTATATACTTGGTATGGAAAGATCCATGTAGATGTACTCATCTTCTTACGTACCTCGGTTGAGTTTGATGATTAATTAATTTCCTTCTCTCCATTGCTTCATTTTGCATGGCATGTGATTTCTGTTTTACGCAAGACGTGACTTCATATGATGGTTAATTAATAATTTTGTAAATTTCAGGATTCTGCTATTATTGTGTTAAACAAACCCCCCAAACTGCCTGTCAAGGTTTAATTCTCATTCTCTTTAATTTGCCTATTTCATTTCTGGTGGTTGCCGGTGGGTGGGGCGGTGGGGTGGGAGGTAATAGTAATTGCTCTGATATTTTGAAAAAATTATGGTATTGTCAGGGGAATCTTCCAGTTCATAATAGTATGGATGCATTGGCGGCTGCAGCATTATCTTATGATTATGATGAGGGTCCCAAGTTGGTAAGCTTGTTTATATTTAGGGAACCTATTTATGTAGATTTTTTTTTTCTCCAAGAAACAAAAACTTTTCATTGAGTTTGAGAAGTTGCGTATTTATATAGATATCATAATTGAGAAATCTTTGTATAAAGTATAAAAACAGCTGAGTAATTATGTNATAATCGGGAGTAAGAGTTTTGTTCCCCTATACTCGAGGGCTTGGGACTTGGAAAACATTAAGGATTTTTGGTATTAAGCTCACCCGAAGGTTCAAACTTGAGACTTATAAGCTGGTATGACCAAGAGACCCAAGCCCTCGCCAACGGAGCCATCTTTTAGGGATCCCGCGGCGCTACTACTTTGTTAGTCATTTGTTTTTACAAATATGGAGATTCGATCTAGTGGAAGCAGATTTTGGTACTGTAGAATAAAAATTCTTGCTTTCGGTTGTAGCAATTACGAGGCATTTATCTCTAGACATGGGATAAATTTGGGTGGATAAAGCTCCTCATTGGTTTTCTTCCTTTCATCCAAAAAATTATCCATCCAACTTTAGTGGAGAGGATGTCATTCAACTGAAGAAGTTAGAGAAATTATGTTTATTCCTTGAATAAGGTCGTACAAGCTAGTATATTTTATTATTTTTCATCTGCAATGGCATTATTGGTTGCCTTGATCTATATCTAATGCTCTCCCTATCTGTTCTTATGGATGCTCATTATTAGGTTCATCGTTTGGATCGAGAGAGCAGCGGGATCCTCTTGATGGGGAGAACAAAAGAAAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCAAAATCTTCATGTAAGGTGCTTAATGCCATAATGCATTTAAACTAAAACAAAAGCAAAGAAGAAGATTAAACTGAGGTTTATCTTCAAGATGCTAATACGTGCTAGCTATGGCTAAAAAATGTTATAGAATTCCTTTACAATTATTGAAGTTTTATAATATGCTCAATGATGTTTTAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTGGTAATTGGTTGTCCAAGGGAAAGAGAAGGCCTAATTTCTGCTCCTCTGTCTAAGGTTCTCTCTCTCTCTGGGATCAATGTTTATGATTCTCACTCAATAGTTGCAGGTGGATTTATATAAAATAATCAGACAACAATATTTACTTGATAAGTTGAAAGTTAGAATACAATCATGTATCAGAATGGTTGATTGATTGACATGTGGTTACTCTGCCTTTAGAAACTTTGAAATCTATCTTCTAGAGCTTGAGAATATAGGTCAAACGAGTCTTTCAGGTATTAGCTCGAGTTGTTCTAACCATGTTATAATTGATTGTAGGTGCTTCTTGACGATGGGAAGACCGAGAGGGTCGTCTTGGCGAGTAAAACATCCTTGGAGGCTTCTCAGGAGGCAATCACTGAGTATCGCGTGCTTGGTCCCACGATAAATGGTTGTTCATGGATTGAACTGTGTCCCCGTACTAGCCGAAAGCATCAGGTACTTGAATCTCGCTCTCTCTCTCTCATGTGCACAAGCTCATGTATTGGTTTGTATCATCTTTATAATTGATCCTGCGAACCATCAAGGAAAGTGTCAATCTGAATTCATCTGATTCTTTGGAGGCAAATACTCCTTCCTATTCTTAAGTACTTCATTTTGTACACATCTGATACTGTCTTTCAGCTTCGGATCCATTGTGCTGAAGCTCTGGGCACACCTATTGTCGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAGATGCCACGTGTCGATGTTGAGCAGATAAGTGGGAAGCCGTACAAGTTGCGGAGGCCAGAAGGGCTGGACGTGCAAAAGGGGAGTGTTTTATCCAAGGTGCCATTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTTATCGACGTTTTGGTTAATAAGAGGTCGATCAGCCAGCACCCAGTATCTAGCTCGAACTCGGATCTCTTACGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTA

mRNA sequence

ATGCAGCAGAGCATACGGCGGCATCTTTTACTCGCAGCCAGATACTACTCCAGAATTTCTCCTCCACCACCTGCATATGCTAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCATTGCCCCCATTTCCTGGTTACCCTTTGCCTCGGAAGAATCATATCGGTATATCTGGGGGGTCAACTCATGTCACTGCCATAAATTGGGTCAAGCACTATTTTGATGAAATTCATGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTCCAGGTGGAATGTCCACAATCGGGTGGATCAAATGGAAAAGGAAATTTGAAACCTATGAGAAAGATTAAGAGTAATGAGATTATGGAAACTGGGGCAAGAGTCCATATACCTGTGTCAGTTGCGGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGTACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTTAAGTACAAGGCAAGTACCAATAATTGCTCTGATATTTTGAAAAAATTATGGTATTGTCAGGGGAATCTTCCAGTTCATAATAGTATGGATGCATTGGCGGCTGCAGCATTATCTTATGATTATGATGAGGGTCCCAAGTTGGTTCATCGTTTGGATCGAGAGAGCAGCGGGATCCTCTTGATGGGGAGAACAAAAGAAAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCAAAATCTTCATGTAAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTGGTAATTGGTTGTCCAAGGGAAAGAGAAGGCCTAATTTCTGCTCCTCTGTCTAAGGTGCTTCTTGACGATGGGAAGACCGAGAGGGTCGTCTTGGCGAGTAAAACATCCTTGGAGGCTTCTCAGGAGGCAATCACTGAGTATCGCGTGCTTGGTCCCACGATAAATGGTTGTTCATGGATTGAACTGTGTCCCCGTACTAGCCGAAAGCATCAGCTTCGGATCCATTGTGCTGAAGCTCTGGGCACACCTATTGTCGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAGATGCCACGTGTCGATGTTGAGCAGATAAGTGGGAAGCCGTACAAGTTGCGGAGGCCAGAAGGGCTGGACGTGCAAAAGGGGAGTGTTTTATCCAAGGTGCCATTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTTATCGACGTTTTGGTTAATAAGAGGTCGATCAGCCAGCACCCAGTATCTAGCTCGAACTCGGATCTCTTACGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTA

Coding sequence (CDS)

ATGCAGCAGAGCATACGGCGGCATCTTTTACTCGCAGCCAGATACTACTCCAGAATTTCTCCTCCACCACCTGCATATGCTAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCATTGCCCCCATTTCCTGGTTACCCTTTGCCTCGGAAGAATCATATCGGTATATCTGGGGGGTCAACTCATGTCACTGCCATAAATTGGGTCAAGCACTATTTTGATGAAATTCATGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTCCAGGTGGAATGTCCACAATCGGGTGGATCAAATGGAAAAGGAAATTTGAAACCTATGAGAAAGATTAAGAGTAATGAGATTATGGAAACTGGGGCAAGAGTCCATATACCTGTGTCAGTTGCGGAGATGAAGATTTCTAGGAGATTTGACACTATACCAAGTGGTACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTTAAGTACAAGGCAAGTACCAATAATTGCTCTGATATTTTGAAAAAATTATGGTATTGTCAGGGGAATCTTCCAGTTCATAATAGTATGGATGCATTGGCGGCTGCAGCATTATCTTATGATTATGATGAGGGTCCCAAGTTGGTTCATCGTTTGGATCGAGAGAGCAGCGGGATCCTCTTGATGGGGAGAACAAAAGAAAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCAAAATCTTCATGTAAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTGGTAATTGGTTGTCCAAGGGAAAGAGAAGGCCTAATTTCTGCTCCTCTGTCTAAGGTGCTTCTTGACGATGGGAAGACCGAGAGGGTCGTCTTGGCGAGTAAAACATCCTTGGAGGCTTCTCAGGAGGCAATCACTGAGTATCGCGTGCTTGGTCCCACGATAAATGGTTGTTCATGGATTGAACTGTGTCCCCGTACTAGCCGAAAGCATCAGCTTCGGATCCATTGTGCTGAAGCTCTGGGCACACCTATTGTCGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAGATGCCACGTGTCGATGTTGAGCAGATAAGTGGGAAGCCGTACAAGTTGCGGAGGCCAGAAGGGCTGGACGTGCAAAAGGGGAGTGTTTTATCCAAGGTGCCATTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTTATCGACGTTTTGGTTAATAAGAGGTCGATCAGCCAGCACCCAGTATCTAGCTCGAACTCGGATCTCTTACGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTA

Protein sequence

MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGNLKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKASTNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV

Homology

[Close]

BLAST of MS001050.1 vs. NCBI nr
Match: XP_022131827.1 (RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 928.3 bits (2398), Expect = 2.6e-266
Identity = 459/474 (96.84%), Postives = 462/474 (97.47%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGL+QVECPQSGGSNGKGN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
           S     +   KL   +GNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT
Sbjct: 181 SAIIVLNKPPKL-PVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473

[Close]

BLAST of MS001050.1 vs. NCBI nr
Match: XP_022131824.1 (RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP_022131825.1 RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP_022131832.1 RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 877.5 bits (2266), Expect = 5.3e-251
Identity = 445/484 (91.94%), Postives = 449/484 (92.77%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLV       GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLV-----NPGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKASTNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK S     +   KL   +GNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKDSAIIVLNKPPKL-PVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 478

[Close]

BLAST of MS001050.1 vs. NCBI nr
Match: XP_022997125.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 874.0 bits (2257), Expect = 5.8e-250
Identity = 427/474 (90.08%), Postives = 450/474 (94.94%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLAARYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEIHDSVIQSHFRKGLVQV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
           S     +   +L   +GNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 SAILVLNKPPRL-PVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LVLPN+AKF++VL +KRSIS H VS+ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473

[Close]

BLAST of MS001050.1 vs. NCBI nr
Match: XP_022131826.1 (RNA pseudouridine synthase 3, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 870.5 bits (2248), Expect = 6.5e-249
Identity = 442/484 (91.32%), Postives = 446/484 (92.15%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK          GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK--------DPGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKASTNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK S     +   KL   +GNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKDSAIIVLNKPPKL-PVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475

[Close]

BLAST of MS001050.1 vs. NCBI nr
Match: XP_022961737.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 869.8 bits (2246), Expect = 1.1e-248
Identity = 426/474 (89.87%), Postives = 448/474 (94.51%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLA RYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEI DSVIQSHFRKGLVQV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
           S     +   +L   +GNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 SAILVLNKPPRL-PVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LVLPN+AKF++VL +KRSIS H VS  NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473

[Close]

BLAST of MS001050.1 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 1.2e-181
Identity = 316/474 (66.67%), Postives = 380/474 (80.17%), Query Frame = 0


Query: 8   HLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKN 67
           +L +  R YSR++PPP +    VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLP KN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  -----HIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVEC--PQSGGSNGKGN 127
                  G SGG  HVTAI+WVK+YF+EI+D  IQ+HF KGLVQ+E    +      +  
Sbjct: 72  AGADGDDGDSGG--HVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDG 131

Query: 128 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 187
             PMRKIK NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++K 
Sbjct: 132 AIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKD 191

Query: 188 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 247
           S     +   KL   +GN+P+HNSMDALAAAALS+  DEGP+LVHRLDRE+SG+L+MGRT
Sbjct: 192 SAIIVLNKPPKL-PVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRT 251

Query: 248 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 307
           KES+ +L  +FS+ +   SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLD
Sbjct: 252 KESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLD 311

Query: 308 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 367
           DGKT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGT
Sbjct: 312 DGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGT 371

Query: 368 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 427
           PIVGDYKYGWFVH+ WKQMP+VD+E  +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 372 PIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 431

Query: 428 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           +VLPNIAKF+ V+  + +   H       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 MVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

[Close]

BLAST of MS001050.1 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 3.4e-160
Identity = 285/477 (59.75%), Postives = 357/477 (74.84%), Query Frame = 0


Query: 6   RRHLLLAARYYSRISPPPPAYAKP-VIRV-SNNIAQLGSPKEGPKPRQLLSLPPFP---- 65
           RR +  A R+ SR++PP PA A P V+RV  +N+A+LG PK GP+PRQLLSLPPFP    
Sbjct: 5   RRRVGAAVRWLSRLAPPAPAEADPVVVRVDGSNVARLGKPKPGPRPRQLLSLPPFPGGGD 64

Query: 66  GYPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGS--NG 125
           G PLP +     +     VTA++WVKHY  ++   V+Q+HF K LV  EC     S    
Sbjct: 65  GDPLPGRK----AAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRLVYSECSDHEVSVETI 124

Query: 126 KGNLKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVK 185
           K     ++KIK N++ME G R+H+PVSVAE +I +R++TIP+ TL+PN DEI+YL+RLV 
Sbjct: 125 KSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYLRRLVI 184

Query: 186 YKASTNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLM 245
           +K S     +   K+   +GNLPVHNSMD LAAAALSY  +EGPKLVHRLDRESSG+LL 
Sbjct: 185 HKDSAILVLNKPPKV-PMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLF 244

Query: 246 GRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKV 305
           GRTKES   L WLF+++  AK++ + W+ AC A  Q+YWALVIG P+EREG+ISAPLSKV
Sbjct: 245 GRTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKV 304

Query: 306 LLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEA 365
           LLDDGK ERV+LA  + ++ +QEA+T YRV+GPTI+GCSWIEL P T RKHQLR+HCAEA
Sbjct: 305 LLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEA 364

Query: 366 LGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLH 425
           LGTPIVGDYKYGWFVH+ WKQ P+ D E  +G+PYKLRRPEGL++QKGSVLSKVPLLHLH
Sbjct: 365 LGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPLLHLH 424

Query: 426 CRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           CRE+V+PNIAKF+     +   +  P S    +LLRF+A MP+HM+ISWN+MSSYLV
Sbjct: 425 CREMVIPNIAKFLS-SNGEWHENGAPWSKEKPNLLRFIAPMPAHMKISWNIMSSYLV 475

[Close]

BLAST of MS001050.1 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.3e-63
Identity = 152/404 (37.62%), Postives = 229/404 (56.68%), Query Frame = 0


Query: 72  SGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQS--GGSNGKGNLKPMRKIKS 131
           S  ++  TA+ W+     ++  +++Q  FR   V+ E   S  G    +  LK   ++ +
Sbjct: 84  STDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQRSQLK---RVAA 143

Query: 132 NEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKASTNNCSDIL 191
            E +  G R+++P+SV     + +       +   + +E K++  LV YK   +    +L
Sbjct: 144 KESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCSLVLYK---DPAIIVL 203

Query: 192 KKL--WYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHL 251
            K      QG   +  S+D LAA+ L +D  E P+LVHRLDR+ SG+L++ RT+ +   L
Sbjct: 204 NKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVL 263

Query: 252 QWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERV 311
             +F   +   +S     +   +  +KY ALVIGCP  + G ISAPL KV++DDGK+ER+
Sbjct: 264 HSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERI 323

Query: 312 VLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYK 371
            +     L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCAE LGTPIVGDYK
Sbjct: 324 TVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYK 383

Query: 372 YGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIA 431
           YGW  H+   + P V  E    K  +   P GLD+  G V SK P LHLH +++ LPNI+
Sbjct: 384 YGWQAHKA--REPFVSSENNPTK--QSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNIS 443

Query: 432 KFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSS 472
           +    L+ K  +S      S+ D L+F A +PSHM++S+NL+ S
Sbjct: 444 Q----LLEKMQVSSDS-DISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of MS001050.1 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.6e-35
Identity = 93/280 (33.21%), Postives = 154/280 (55.00%), Query Frame = 0


Query: 74  GSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQ--VECPQSGGSNGKGNLKPMRKIKSNE 133
           G    TAI WV+    ++  S++Q  FR   V+  V   +   ++       +R++ + +
Sbjct: 80  GEQGATAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKD 139

Query: 134 IMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKASTNNCSDILKK 193
            +  G  + +PV++ E  ++ +         + N +EI +L+ L  YK   +    ++ K
Sbjct: 140 QLMPGDILFLPVNLKESSVAEKTKK------FDNRNEINFLRGLEIYK---DEAIIVVNK 199

Query: 194 L--WYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHLQW 253
                 QG + + NS+D L A+    +  E P+LVHRLDR+ SGIL++GR + S + L  
Sbjct: 200 PPGMPVQGGVGIKNSIDVL-ASMFEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLHA 259

Query: 254 LFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVL 313
           +F      K++    D   +   +KY ALVIG PR  +GL+SAPL+K+LL DGK+ER+ +
Sbjct: 260 IF----REKTADALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTI 319

Query: 314 ASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQ 350
            + ++  + Q+A+TEYRV+     G +W+EL PRT RKHQ
Sbjct: 320 RASSNAASVQDALTEYRVIESCPQGYTWLELFPRTGRKHQ 345

[Close]

BLAST of MS001050.1 vs. ExPASy Swiss-Prot
Match: Q28C59 (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.8e-20
Identity = 77/250 (30.80%), Postives = 112/250 (44.80%), Query Frame = 0


Query: 197 GNLPVHNSMDALAAAALSYDYD---EGPKLVHRLDRESSGILLMGRTKESVAHLQWLFSN 256
           G   V  S+ +L  A   + +    E  KL HRLDR+++G L++ RT E+   +Q     
Sbjct: 71  GGPTVERSVASLLPALAKHHFGWKAEPLKLCHRLDRDTTGALILARTTEAAERVQQALRE 130

Query: 257 IRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKV---------------- 316
                             ++ YWAL +G P  REG++  P+ +                 
Sbjct: 131 ---------------REVHRVYWALCLGTPSPREGILDIPIMEKETSGPQKHYKMALSPR 190

Query: 317 --LLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCA 376
             + ++G  ERV +       ++ EA+T YR LG   +G S +EL P T  KHQLR+H A
Sbjct: 191 FRVSEEGAVERVRVP-----RSAHEAVTRYRTLG-AASGASLVELHPITGVKHQLRVHLA 250

Query: 377 EALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLH 426
             L  PI+GD+KY  +  R   Q P          P  + R  GL V +   LS    LH
Sbjct: 251 LGLNCPILGDHKYSHW-GRLAPQKP----------PDSVLRALGLTVPQARTLS----LH 284

[Close]

BLAST of MS001050.1 vs. ExPASy TrEMBL
Match: A0A6J1BS40 (RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.3e-266
Identity = 459/474 (96.84%), Postives = 462/474 (97.47%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGL+QVECPQSGGSNGKGN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
           S     +   KL   +GNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT
Sbjct: 181 SAIIVLNKPPKL-PVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 473

[Close]

BLAST of MS001050.1 vs. ExPASy TrEMBL
Match: A0A6J1BQS0 (RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.6e-251
Identity = 445/484 (91.94%), Postives = 449/484 (92.77%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLV       GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLV-----NPGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKASTNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK S     +   KL   +GNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKDSAIIVLNKPPKL-PVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 478

[Close]

BLAST of MS001050.1 vs. ExPASy TrEMBL
Match: A0A6J1K8P8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492130 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 427/474 (90.08%), Postives = 450/474 (94.94%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLAARYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEIHDSVIQSHFRKGLVQV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
           S     +   +L   +GNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 SAILVLNKPPRL-PVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LVLPN+AKF++VL +KRSIS H VS+ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSNLNSDLLRFVARMPSHMRISWNLMSSYLV 473

[Close]

BLAST of MS001050.1 vs. ExPASy TrEMBL
Match: A0A6J1BQJ8 (RNA pseudouridine synthase 3, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.1e-249
Identity = 442/484 (91.32%), Postives = 446/484 (92.15%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK          GG +  G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRK--------DPGGMSTIGW 120

Query: 121 LKPMRK----------IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          IKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKASTNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240
           YLQRLVKYK S     +   KL   +GNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE
Sbjct: 181 YLQRLVKYKDSAIIVLNKPPKL-PVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRE 240

Query: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300
           SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI
Sbjct: 241 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 300

Query: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360
           SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL
Sbjct: 301 SAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQL 360

Query: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420
           RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK
Sbjct: 361 RIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSK 420

Query: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475
           VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 VPLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMS 475

[Close]

BLAST of MS001050.1 vs. ExPASy TrEMBL
Match: A0A6J1HAX8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111462419 PE=4 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 5.3e-249
Identity = 426/474 (89.87%), Postives = 448/474 (94.51%), Query Frame = 0


Query: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLLA RYYSRISPPPPAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSNGKGN 120
           YPLPRKN IG SG STHVTAI WVKHYFDEI DSVIQSHFRKGLVQV+CPQSGG +G+GN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI+SNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYK 
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 240
           S     +   +L   +GNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRESSGI+LMGRT
Sbjct: 181 SAILVLNKPPRL-PVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRT 240

Query: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300
           KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD
Sbjct: 241 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 300

Query: 301 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360
           DGKTERVVLA+KTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT
Sbjct: 301 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 360

Query: 361 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 420
           PIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCRE
Sbjct: 361 PIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCRE 420

Query: 421 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LVLPN+AKF++VL +KRSIS H VS  NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 LVLPNLAKFLNVLDDKRSISHHRVSYFNSDLLRFVARMPSHMRISWNLMSSYLV 473

[Close]

BLAST of MS001050.1 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 637.5 bits (1643), Expect = 8.6e-183
Identity = 316/474 (66.67%), Postives = 380/474 (80.17%), Query Frame = 0


Query: 8   HLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPRKN 67
           +L +  R YSR++PPP +    VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLP KN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  -----HIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVEC--PQSGGSNGKGN 127
                  G SGG  HVTAI+WVK+YF+EI+D  IQ+HF KGLVQ+E    +      +  
Sbjct: 72  AGADGDDGDSGG--HVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDG 131

Query: 128 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKA 187
             PMRKIK NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++K 
Sbjct: 132 AIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKD 191

Query: 188 STNNCSDILKKLWYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRT 247
           S     +   KL   +GN+P+HNSMDALAAAALS+  DEGP+LVHRLDRE+SG+L+MGRT
Sbjct: 192 SAIIVLNKPPKL-PVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRT 251

Query: 248 KESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 307
           KES+ +L  +FS+ +   SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLD
Sbjct: 252 KESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLD 311

Query: 308 DGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 367
           DGKT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGT
Sbjct: 312 DGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGT 371

Query: 368 PIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 427
           PIVGDYKYGWFVH+ WKQMP+VD+E  +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE
Sbjct: 372 PIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 431

Query: 428 LVLPNIAKFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSSYLV 475
           +VLPNIAKF+ V+  + +   H       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 MVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

[Close]

BLAST of MS001050.1 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 245.4 bits (625), Expect = 9.5e-65
Identity = 152/404 (37.62%), Postives = 229/404 (56.68%), Query Frame = 0


Query: 72  SGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQS--GGSNGKGNLKPMRKIKS 131
           S  ++  TA+ W+     ++  +++Q  FR   V+ E   S  G    +  LK   ++ +
Sbjct: 84  STDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQRSQLK---RVAA 143

Query: 132 NEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKASTNNCSDIL 191
            E +  G R+++P+SV     + +       +   + +E K++  LV YK   +    +L
Sbjct: 144 KESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCSLVLYK---DPAIIVL 203

Query: 192 KKL--WYCQGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTKESVAHL 251
            K      QG   +  S+D LAA+ L +D  E P+LVHRLDR+ SG+L++ RT+ +   L
Sbjct: 204 NKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVL 263

Query: 252 QWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERV 311
             +F   +   +S     +   +  +KY ALVIGCP  + G ISAPL KV++DDGK+ER+
Sbjct: 264 HSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERI 323

Query: 312 VLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYK 371
            +     L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCAE LGTPIVGDYK
Sbjct: 324 TVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYK 383

Query: 372 YGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIA 431
           YGW  H+   + P V  E    K  +   P GLD+  G V SK P LHLH +++ LPNI+
Sbjct: 384 YGWQAHKA--REPFVSSENNPTK--QSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNIS 443

Query: 432 KFIDVLVNKRSISQHPVSSSNSDLLRFVARMPSHMRISWNLMSS 472
           +    L+ K  +S      S+ D L+F A +PSHM++S+NL+ S
Sbjct: 444 Q----LLEKMQVSSDS-DISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of MS001050.1 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 2.3e-10
Identity = 74/364 (20.33%), Postives = 142/364 (39.01%), Query Frame = 0


Query: 63  LPRKNHIG------ISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLVQVECPQSGGSN 122
           +PR N+ G      +      +   +W+    + +  + +QS  R GLV V        N
Sbjct: 61  IPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTV--------N 120

Query: 123 GKGNLKPMRKIKSNEIMETGARVHIPVSVAEMK-ISRRFDTIPSGTLYPNAD--EIKYLQ 182
           G+   K    +KS +         +  +++E++ +    + IP   +Y +     +    
Sbjct: 121 GRVVDKVSHNVKSGD--------EVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPA 180

Query: 183 RLVKYKASTNNCSDILKK-LWYC--------------QGNLPVHNSMDALAAAALSYDYD 242
            +V + A  N    ++   L +C                +    +  + +  +  SY   
Sbjct: 181 HMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYAAS 240

Query: 243 EGPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWAL 302
             P +VHRLD+ ++G+L++ + + S AHL   F                 +   + Y +L
Sbjct: 241 VRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFK---------------LHTIERVYVSL 300

Query: 303 VIGCPREREGLISAPLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCS-W 362
             G P   +G I  P+ +      +     +        ++ A + Y+V+     G S  
Sbjct: 301 TTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRARHAASRYKVIETFAGGGSAL 360

Query: 363 IELCPRTSRKHQLRIHCAEALGTPIVGDYKYG----WFVHRTWKQMPRVDVEQISGKPYK 398
           +E    T R HQ+R H A+ +G P++GD  YG      +    K++ R D E+I     +
Sbjct: 361 VEWRLETGRTHQIRAH-AKYMGVPLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISR 390

The following BLAST results are available for this feature:

BLAST of MS001050.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131827.1	2.6e-266	96.84	RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia]	[more]
XP_022131824.1	5.3e-251	91.94	RNA pseudouridine synthase 3, mitochondrial isoform X1 [Momordica charantia] >XP...	[more]
XP_022997125.1	5.8e-250	90.08	RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 R...	[more]
XP_022131826.1	6.5e-249	91.32	RNA pseudouridine synthase 3, mitochondrial isoform X2 [Momordica charantia]	[more]
XP_022961737.1	1.1e-248	89.87	RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1...	[more]

BLAST of MS001050.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XET6	1.2e-181	66.67	RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q0J4D4	3.4e-160	59.75	RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3...	[more]
Q9LT72	1.3e-63	37.62	RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q69K07	2.6e-35	33.21	RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3...	[more]
Q28C59	1.8e-20	30.80	Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN...	[more]

BLAST of MS001050.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BS40	1.3e-266	96.84	RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX...	[more]
A0A6J1BQS0	2.6e-251	91.94	RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX...	[more]
A0A6J1K8P8	2.8e-250	90.08	RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1BQJ8	3.1e-249	91.32	RNA pseudouridine synthase 3, mitochondrial isoform X2 OS=Momordica charantia OX...	[more]
A0A6J1HAX8	5.3e-249	89.87	RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of MS001050.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78910.1	8.6e-183	66.67	Pseudouridine synthase family protein	[more]
AT3G19440.1	9.5e-65	37.62	Pseudouridine synthase family protein	[more]
AT1G76050.2	2.3e-10	20.33	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 204..355 e-value: 2.5E-19 score: 69.9
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 198..369 e-value: 2.02166E-37 score: 133.617
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 194..431 e-value: 3.3E-41 score: 143.3
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 6..471
None	No IPR available	PANTHER	PTHR21600:SF53	RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIAL	coord: 6..471
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 201..426

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS001050	MS001050	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS001050.1-cds	MS001050.1-cds-scaffold36:1073888..1074262	CDS
MS001050.1-cds	MS001050.1-cds-scaffold36:1074449..1074607	CDS
MS001050.1-cds	MS001050.1-cds-scaffold36:1074872..1074973	CDS
MS001050.1-cds	MS001050.1-cds-scaffold36:1075136..1075252	CDS
MS001050.1-cds	MS001050.1-cds-scaffold36:1075957..1076077	CDS
MS001050.1-cds	MS001050.1-cds-scaffold36:1076471..1076640	CDS
MS001050.1-cds	MS001050.1-cds-scaffold36:1076780..1076845	CDS
MS001050.1-cds	MS001050.1-cds-scaffold36:1077306..1077617	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS001050.1	MS001050.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding