MS000997.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS000997.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	6,7-dimethyl-8-ribityllumazine synthase
Location	scaffold36: 729843 .. 732600 (+)
Sequence length	651
RNA-Seq Expression	MS000997.1
Synteny	MS000997.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCTTCATTTGCTTCTCCGGCTCAGGGCTTGCTTCATCGCCAGCATCTGCCGCTCGCATTTACCAATTCCAACTTCAATCGCACTCAACTTCAATTTCATGGGGCGACTCCTGCGAAATCACAACCCCTCTCTTTTTCCTCCCAATTAAAGCTTTCTAATGATGCCTCTCCGAGGAACGATCACCGTTCCTCCTTTGCGCAGACGGCTGCCGTCCGCCATATCGTCGGTTCTCTTTCCAAAGCCGAGGGCCTCCGCTTTGCTGTGGTAATTTCTCTTTTTCCTCTGTCTTTCCCGCTCTGTTTTTGTCGCTTTATAGTTCAGCGTTATCTGCCCCTTTTGCTCCACACATTTTGACATGTGGAAGTTCTCTTCGTTCTTGAGGTTACGCCGATGGATTTTTGTTCATTGTTCCATATTTACGAAATGCCGGCGTCTGAAGTGGTTTTTCCCAGTCGTCTACATAAATAGTTGTAGTCTAGTTGAGAAACATACGTCTGGATGATGCAGTCTCATCTGAGAGATTATTCGTCACTTCCATACCTGTATCCATAATTAAGAAGCGTTCTTTTGGTTTGATGCTTGCATCGAATTCAAAGGTTATGAATGTGGTGGTCACATTTGTGGCTGCGGCTATGAGTAATCTCTCGGCTCGGTTACTTCAACTGAGAGCCTTATTGGCTGTGAAACATACCTGTGGATGAGAATTTGAAATTCTAGGGATTGCGATGATTTGCCCGTGTATTCGTGGTGATGTATCAGATTAAACTTCTTCCAAAATTTGTCGCTGCAGCCGTCCGAGCGAATTATGTTGTCAGGAGTCTGAATTGATTTTTGTTTTTAGATTTTTTAACGAACATCCATTTCTTTCATGTTTGCAAATCCCGTGATGGGCTTCTCAATACTGAAGTTTTATTTATTTTGCTTTGATGAAGGTTGTGGGGCGGTTCAACGAAATTGTGACAAGGCCAATGCTGGAGGGAGCTTTGTCTACATTCAAAAGTTATTCAGTTCAAGACGAGGATATTGATGTAAGCGTTTCAATATACGACATGTTAAGTCGCATGTTCTTTTTTTTTTCTTTCTATTTTGCTAATGTTGGAAACTGAGCAAGTTTGTGAAGAAATATGATCTGAAACTAGATTCTCGTCATCTCGTGGATGACACAGAATATAAATAAACAAATTTAACTATATATAATGGGTTTAAATAGAATAGGGTGAAAGAGTACGATTGACAGATGACTAGCAGAATGTGTATCTGATATCTAGTAATTGGTATGCAGCTTTAACTTTGCTTGAGCCTTGACGTGTTGACTTCATTATTTACCTCGCTATGACATCAGGTTGTATGGGTTCCTGGGAGCTTTGATATTCCTGTAGTCGCTGAAAGGCTTGGGAAATCTGGAAAGTACCATGCTATTTTATGTATTGGAGCTGTGGTATGATTTCCTGTTACACGTGTCAAGTCCTCTTATATATTTTAGTCTCCGGTGCACTTATTAGTATTACCAACCACCTCTTTATGTAGATTAAAGGTGATACATCTCATTATGATGCCGTCGTGAACTCTACTGCATCTGGAGTGCTTTCTGCCGGGCTGAAGTCAGGTTGGTTTTCTTGTTTGTGTGATTTACTAGAATTTTATGTTCTTTAACCCTGTTAGTTGAAGTTTTGACTTACCAATTTTGCTTTTGCATCGCATATATAGCTTTTATAAGTACAACCTCAAGGGTAGAGCATAGTGGTTAAGGTTTTTTAATCCAATTGATATATTCAAGAATTTTGGGGACGTCTCATTTTGAGATATTTGAAATCGTATGGGTTTTTAAATGCTAAAAATTGCAGTGAAGTTGACTTAATAGTTCCAGTTTTGATCTCAATGAAAAGTGAGGGACTAAGGAATTTCAGGGTATGGAAAGGAAAAGAGAATCTGGTTGTTGTAGATTCATAAGTAGATGAAGGTTAATATAACTTGTGCCATGATATGCACATTCCTTGCTTCTTTTTTATTTTCTTTTTTTTCTAAATAGAAAATGACGTCTCCATTTAGAGCCTATATTTCTCCCTCGATCGCATGATAGTTCTATTCTCATTATTAATTTAAGGTGAAAATGTCAAAGACCTTGGTCATTCTTCAAAGTATCTTTTTTTTTTTTAAAGGTAATATATCATTGGAGAAAGGTCCCTGTTAAAGCATTTTACTATAGGCCTTGGTAATAGTTCGATGTGGAAGAAGAATGGTTTTACGTTTAAGCTGTTGGGAGCTTGCTTAATAGTGAATCGTGTTTTTGTTTTATAATTGCGATGAAAATTATCTTTAAGCACTGTTATATGTGCAAGCCTGTGTTGAAATTTATTGAAGGCTACAATGTTGAACTTGTGTTTGATGACTACAAGTAATCTCTGTAATCATTTATTTAGGGTAATACTACTTATATGAGTCAGAAAATTGTCGGTGTTGCCATTTTATTTCCTCTCTTTCATCTTTTAGCTGACACCGGATTAAATCTAAGCCATTTTCTTATGTTTCTGTAGGAGTTCCGTGCGTATTTAGTGTCTTGACTTGTGACAACTTAGATCAGGTAAATATGATCTAAACTGAGTGTTGAGCAATATCCTGCCTAGAGAATTGTTTTTACAGGAGCTATGATTTCTGATGATTATATATTTTTACTGCTTCTTTCTTGAAGGCTTTCAATCGAGCTGGTGGTAAATCTGGAAATAAGGGTTCTGAGGGTGCTTTGACAGCTGTA

mRNA sequence

ATGGCCGCTTCATTTGCTTCTCCGGCTCAGGGCTTGCTTCATCGCCAGCATCTGCCGCTCGCATTTACCAATTCCAACTTCAATCGCACTCAACTTCAATTTCATGGGGCGACTCCTGCGAAATCACAACCCCTCTCTTTTTCCTCCCAATTAAAGCTTTCTAATGATGCCTCTCCGAGGAACGATCACCGTTCCTCCTTTGCGCAGACGGCTGCCGTCCGCCATATCGTCGGTTCTCTTTCCAAAGCCGAGGGCCTCCGCTTTGCTGTGGTTGTGGGGCGGTTCAACGAAATTGTGACAAGGCCAATGCTGGAGGGAGCTTTGTCTACATTCAAAAGTTATTCAGTTCAAGACGAGGATATTGATGTTGTATGGGTTCCTGGGAGCTTTGATATTCCTGTAGTCGCTGAAAGGCTTGGGAAATCTGGAAAGTACCATGCTATTTTATGTATTGGAGCTGTGATTAAAGGTGATACATCTCATTATGATGCCGTCGTGAACTCTACTGCATCTGGAGTGCTTTCTGCCGGGCTGAAGTCAGGAGTTCCGTGCGTATTTAGTGTCTTGACTTGTGACAACTTAGATCAGGCTTTCAATCGAGCTGGTGGTAAATCTGGAAATAAGGGTTCTGAGGGTGCTTTGACAGCTGTA

Coding sequence (CDS)

ATGGCCGCTTCATTTGCTTCTCCGGCTCAGGGCTTGCTTCATCGCCAGCATCTGCCGCTCGCATTTACCAATTCCAACTTCAATCGCACTCAACTTCAATTTCATGGGGCGACTCCTGCGAAATCACAACCCCTCTCTTTTTCCTCCCAATTAAAGCTTTCTAATGATGCCTCTCCGAGGAACGATCACCGTTCCTCCTTTGCGCAGACGGCTGCCGTCCGCCATATCGTCGGTTCTCTTTCCAAAGCCGAGGGCCTCCGCTTTGCTGTGGTTGTGGGGCGGTTCAACGAAATTGTGACAAGGCCAATGCTGGAGGGAGCTTTGTCTACATTCAAAAGTTATTCAGTTCAAGACGAGGATATTGATGTTGTATGGGTTCCTGGGAGCTTTGATATTCCTGTAGTCGCTGAAAGGCTTGGGAAATCTGGAAAGTACCATGCTATTTTATGTATTGGAGCTGTGATTAAAGGTGATACATCTCATTATGATGCCGTCGTGAACTCTACTGCATCTGGAGTGCTTTCTGCCGGGCTGAAGTCAGGAGTTCCGTGCGTATTTAGTGTCTTGACTTGTGACAACTTAGATCAGGCTTTCAATCGAGCTGGTGGTAAATCTGGAAATAAGGGTTCTGAGGGTGCTTTGACAGCTGTA

Protein sequence

MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPRNDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDEDIDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKSGVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV

Homology

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BLAST of MS000997.1 vs. NCBI nr
Match: XP_022132287.1 (6,7-dimethyl-8-ribityllumazine synthase, chloroplastic [Momordica charantia] >AFZ99646.1 lumazine synthase [Momordica charantia])

HSP 1 Score: 419.5 bits (1077), Expect = 1.8e-113
Identity = 213/217 (98.16%), Postives = 215/217 (99.08%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MAASFASPAQGLLHRQHLP+AFT SNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR
Sbjct: 1   MAASFASPAQGLLHRQHLPIAFTTSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
           NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG SGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGNSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTA+
Sbjct: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAM 217

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BLAST of MS000997.1 vs. NCBI nr
Match: XP_038884169.1 (6,7-dimethyl-8-ribityllumazine synthase, chloroplastic [Benincasa hispida])

HSP 1 Score: 379.0 bits (972), Expect = 2.7e-101
Identity = 191/217 (88.02%), Postives = 205/217 (94.47%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MAASFA+PAQGL+HRQ+L LAF+NSNFNRTQLQF G   AKSQP SFSSQLKL N ASP 
Sbjct: 1   MAASFAAPAQGLVHRQNLTLAFSNSNFNRTQLQFQGTLSAKSQPFSFSSQLKLPNVASPT 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
           NDHRSSF QTAAVRHIVGSL+KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  NDHRSSFVQTAAVRHIVGSLAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           +DVVWVPGSFDIPVVAERLG+SGKYHA+LCIGAVI+GDTSHYDAVVNS+ASGVLSAGL S
Sbjct: 121 VDVVWVPGSFDIPVVAERLGRSGKYHAVLCIGAVIRGDTSHYDAVVNSSASGVLSAGLNS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           GVPCVFSVLTCDNL+QAF+RAGGK+GNKGSEGALTA+
Sbjct: 181 GVPCVFSVLTCDNLEQAFDRAGGKAGNKGSEGALTAM 217

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BLAST of MS000997.1 vs. NCBI nr
Match: XP_023002869.1 (6,7-dimethyl-8-ribityllumazine synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-98
Identity = 188/217 (86.64%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MA SFA  AQGL++RQ+L LAF+NSNFNRTQLQF G  PAKSQ LSFSSQLKL+NDASPR
Sbjct: 1   MAVSFAGAAQGLVYRQNLTLAFSNSNFNRTQLQFQGQNPAKSQSLSFSSQLKLANDASPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
             HRSSFAQT AVRHIVGSL+KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  KHHRSSFAQTDAVRHIVGSLAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG+SGKYHA+LCIGAVI+GDTSHYDAVVNS ASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGRSGKYHAVLCIGAVIRGDTSHYDAVVNSAASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           G+PCVFSVLT +NL+QAF+RAGGK+GNKGSEGALTA+
Sbjct: 181 GIPCVFSVLTTENLEQAFDRAGGKAGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. NCBI nr
Match: XP_022951341.1 (6,7-dimethyl-8-ribityllumazine synthase, chloroplastic-like [Cucurbita moschata] >KAG6585234.1 6,7-dimethyl-8-ribityllumazine synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020155.1 6,7-dimethyl-8-ribityllumazine synthase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-98
Identity = 188/217 (86.64%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MA SFA  AQGL++RQ+L LAF+NSNFNRTQLQF G  PAKSQ LSFSSQLKL+NDASPR
Sbjct: 1   MAVSFAGAAQGLVYRQNLTLAFSNSNFNRTQLQFQGPNPAKSQSLSFSSQLKLANDASPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
             HRSSFAQT AVRHIVGSL+KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  KHHRSSFAQTDAVRHIVGSLAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG+SGKYHA+LCIGAVI+GDTSHYDAVVNS ASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGRSGKYHAVLCIGAVIRGDTSHYDAVVNSAASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           G+PCVFSVLT +NL+QAF+RAGGK+GNKGSEGALTA+
Sbjct: 181 GIPCVFSVLTTENLEQAFDRAGGKAGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. NCBI nr
Match: XP_023537234.1 (6,7-dimethyl-8-ribityllumazine synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368.6 bits (945), Expect = 3.6e-98
Identity = 187/217 (86.18%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MA SFA  AQGL++RQ+L LAF+NSNFNRT+LQF G  PAKSQ LSFSSQLKL+NDASPR
Sbjct: 1   MAVSFAGAAQGLVYRQNLTLAFSNSNFNRTKLQFQGPNPAKSQSLSFSSQLKLANDASPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
             HRSSFAQT AVRHIVGSL+KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  KHHRSSFAQTDAVRHIVGSLAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG+SGKYHA+LCIGAVI+GDTSHYDAVVNS ASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGRSGKYHAVLCIGAVIRGDTSHYDAVVNSAASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           G+PCVFSVLT +NL+QAF+RAGGK+GNKGSEGALTA+
Sbjct: 181 GIPCVFSVLTTENLEQAFDRAGGKAGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. ExPASy Swiss-Prot
Match: O80575 (6,7-dimethyl-8-ribityllumazine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g44050 PE=1 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 4.7e-64
Identity = 131/221 (59.28%), Postives = 164/221 (74.21%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPA-KSQPLSFSSQLKLSNDASP 60
           M +  + P   L+   H  L   NS  + +    HG +   KS  LSFSS    S  ASP
Sbjct: 1   MKSLASPPCLRLIPTAHRQL---NSRQSSSACYIHGGSSVNKSNNLSFSS--STSGFASP 60

Query: 61  ---RNDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSV 120
                + RSSF QTAAVRH+ GSL + EGLRFA+VV RFNE+VT+ +LEGA+ TFK YSV
Sbjct: 61  LAVEKELRSSFVQTAAVRHVTGSLIRGEGLRFAIVVARFNEVVTKLLLEGAIETFKKYSV 120

Query: 121 QDEDIDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSA 180
           ++EDI+V+WVPGSF+I VVA+ LGKSGK+HA+LCIGAVI+GDT+HYDAV NS ASGVLSA
Sbjct: 121 REEDIEVIWVPGSFEIGVVAQNLGKSGKFHAVLCIGAVIRGDTTHYDAVANSAASGVLSA 180

Query: 181 GLKSGVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
            + SGVPC+F VLTC+++DQA NR+GGK+GNKG+E ALTA+
Sbjct: 181 SINSGVPCIFGVLTCEDMDQALNRSGGKAGNKGAETALTAL 216

[Close]

BLAST of MS000997.1 vs. ExPASy Swiss-Prot
Match: Q9XH32 (6,7-dimethyl-8-ribityllumazine synthase, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.5e-59
Identity = 119/179 (66.48%), Postives = 144/179 (80.45%), Query Frame = 0


Query: 42  SQPLSFSSQLK---LSNDASPRNDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEI 101
           S PLS SS      L + AS +  +R+SF  T AVR + G ++KA+  RFA+VV RFNE 
Sbjct: 34  SAPLSSSSSFPGCGLVHVASNKK-NRASFVVTNAVRELEGYVTKAQSFRFAIVVARFNEF 93

Query: 102 VTRPMLEGALSTFKSYSVQDEDIDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGD 161
           VTR ++EGAL TFK YSV +EDIDVVWVPG++++ V A+ LGKSGKYHAI+C+GAV+KGD
Sbjct: 94  VTRRLMEGALDTFKKYSV-NEDIDVVWVPGAYELGVTAQALGKSGKYHAIVCLGAVVKGD 153

Query: 162 TSHYDAVVNSTASGVLSAGLKSGVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           TSHYDAVVNS +SGVLSAGL SGVPCVF VLTCDN+DQA NRAGGK+GNKG+E ALTA+
Sbjct: 154 TSHYDAVVNSASSGVLSAGLNSGVPCVFGVLTCDNMDQAINRAGGKAGNKGAESALTAI 210

[Close]

BLAST of MS000997.1 vs. ExPASy Swiss-Prot
Match: A6LSS9 (6,7-dimethyl-8-ribityllumazine synthase OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) OX=290402 GN=ribH PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 7.5e-38
Identity = 69/135 (51.11%), Postives = 101/135 (74.81%), Query Frame = 0


Query: 83  AEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDEDIDVVWVPGSFDIPVVAERLGKS 142
           +EGL+F +VVGRFNE +   +LEGA+   K + V++EDID+ WVPG+F+IP++A+++ KS
Sbjct: 10  SEGLKFGIVVGRFNEFIGGKLLEGAIDGLKRHGVKEEDIDIAWVPGAFEIPLIAKKMAKS 69

Query: 143 GKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKSGVPCVFSVLTCDNLDQAFNRAG 202
            KY  ++C+GAVIKG TSHYD V +  + G+ S  L+SG P +F VLT + ++QA  RAG
Sbjct: 70  PKYDGVICLGAVIKGSTSHYDYVCSEVSKGIASVSLESGKPVIFGVLTTNTIEQAIERAG 129

Query: 203 GKSGNKGSEGALTAV 218
            K+GNKG E A++A+
Sbjct: 130 TKAGNKGYESAVSAI 144

[Close]

BLAST of MS000997.1 vs. ExPASy Swiss-Prot
Match: A5D1C7 (6,7-dimethyl-8-ribityllumazine synthase OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) OX=370438 GN=ribH PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.3e-37
Identity = 70/143 (48.95%), Postives = 101/143 (70.63%), Query Frame = 0


Query: 75  HIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDEDIDVVWVPGSFDIPV 134
           H++G     +GLRF +V+GRFNE +T  +L GAL     + V D+DI+V WVPG+F+IP+
Sbjct: 8   HLIG-----QGLRFGIVIGRFNEFITNKLLSGALDALNRHGVADQDIEVAWVPGAFEIPM 67

Query: 135 VAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKSGVPCVFSVLTCDNL 194
           VA ++  + KY A++C+GAVI+G T H+D V    A GV   GL+SGVP +F V+T D++
Sbjct: 68  VARKMASAMKYDAVICLGAVIRGATPHFDYVAGEVAKGVARIGLESGVPTIFGVITADSI 127

Query: 195 DQAFNRAGGKSGNKGSEGALTAV 218
           +QA  RAG K+GNKG + A+TA+
Sbjct: 128 EQAIERAGAKAGNKGWDAAVTAI 145

[Close]

BLAST of MS000997.1 vs. ExPASy Swiss-Prot
Match: B2TJ82 (6,7-dimethyl-8-ribityllumazine synthase OS=Clostridium botulinum (strain Eklund 17B / Type B) OX=935198 GN=ribH PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.4e-36
Identity = 69/135 (51.11%), Postives = 100/135 (74.07%), Query Frame = 0


Query: 83  AEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDEDIDVVWVPGSFDIPVVAERLGKS 142
           AEGL+  ++VGRFNE +   +L+GAL  FK + V  EDIDV WVPG+F++P++A+++ KS
Sbjct: 10  AEGLKVGIIVGRFNEFIGSKLLDGALDGFKRHGVNVEDIDVAWVPGAFEMPLIAKKMAKS 69

Query: 143 GKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKSGVPCVFSVLTCDNLDQAFNRAG 202
            KY A++C+GAVIKG TSHYD V +  + G+ +  L++G P +F VLT +N++QA  RAG
Sbjct: 70  PKYDAVICLGAVIKGSTSHYDYVCSEVSKGIANVSLETGKPVMFGVLTTNNIEQAIERAG 129

Query: 203 GKSGNKGSEGALTAV 218
            K+GNKG E A+ A+
Sbjct: 130 TKAGNKGYECAVGAI 144

[Close]

BLAST of MS000997.1 vs. ExPASy TrEMBL
Match: L0C9N6 (6,7-dimethyl-8-ribityllumazine synthase OS=Momordica charantia OX=3673 GN=LOC111005179 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 8.7e-114
Identity = 213/217 (98.16%), Postives = 215/217 (99.08%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MAASFASPAQGLLHRQHLP+AFT SNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR
Sbjct: 1   MAASFASPAQGLLHRQHLPIAFTTSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
           NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG SGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGNSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTA+
Sbjct: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. ExPASy TrEMBL
Match: A0A6J1KRP6 (6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita maxima OX=3661 GN=LOC111496605 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.1e-99
Identity = 188/217 (86.64%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MA SFA  AQGL++RQ+L LAF+NSNFNRTQLQF G  PAKSQ LSFSSQLKL+NDASPR
Sbjct: 1   MAVSFAGAAQGLVYRQNLTLAFSNSNFNRTQLQFQGQNPAKSQSLSFSSQLKLANDASPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
             HRSSFAQT AVRHIVGSL+KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  KHHRSSFAQTDAVRHIVGSLAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG+SGKYHA+LCIGAVI+GDTSHYDAVVNS ASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGRSGKYHAVLCIGAVIRGDTSHYDAVVNSAASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           G+PCVFSVLT +NL+QAF+RAGGK+GNKGSEGALTA+
Sbjct: 181 GIPCVFSVLTTENLEQAFDRAGGKAGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. ExPASy TrEMBL
Match: A0A6J1GHB3 (6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita moschata OX=3662 GN=LOC111454202 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.1e-99
Identity = 188/217 (86.64%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MA SFA  AQGL++RQ+L LAF+NSNFNRTQLQF G  PAKSQ LSFSSQLKL+NDASPR
Sbjct: 1   MAVSFAGAAQGLVYRQNLTLAFSNSNFNRTQLQFQGPNPAKSQSLSFSSQLKLANDASPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
             HRSSFAQT AVRHIVGSL+KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  KHHRSSFAQTDAVRHIVGSLAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG+SGKYHA+LCIGAVI+GDTSHYDAVVNS ASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGRSGKYHAVLCIGAVIRGDTSHYDAVVNSAASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           G+PCVFSVLT +NL+QAF+RAGGK+GNKGSEGALTA+
Sbjct: 181 GIPCVFSVLTTENLEQAFDRAGGKAGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. ExPASy TrEMBL
Match: A0A6J1KDC4 (6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492217 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 2.5e-97
Identity = 184/217 (84.79%), Postives = 199/217 (91.71%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MAASF +PAQGL+H ++  L F++S FNRTQLQF G  PAKS PLSFS QLKL+ND  PR
Sbjct: 1   MAASFTAPAQGLVHCRNPTLGFSDSIFNRTQLQFQGPVPAKSHPLSFSYQLKLANDVFPR 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
            DHR SF QTAAVRHIVGSL+KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  KDHRCSFVQTAAVRHIVGSLAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG+SGKYHA+LCIGAVI+GDTSHYDAVV+S+ASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGRSGKYHAVLCIGAVIRGDTSHYDAVVSSSASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           G+PCVFSVLTCDNLDQAFNRAGGK GNKGSEGALTA+
Sbjct: 181 GIPCVFSVLTCDNLDQAFNRAGGKHGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. ExPASy TrEMBL
Match: A0A6J1HD10 (6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita moschata OX=3662 GN=LOC111462852 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.4e-97
Identity = 184/217 (84.79%), Postives = 198/217 (91.24%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPAKSQPLSFSSQLKLSNDASPR 60
           MAASF +PAQGL+H ++  L F++S FNRTQLQF G  PAKS PLSFS QLKL+ND SP 
Sbjct: 1   MAASFTAPAQGLVHCRNPTLGFSDSIFNRTQLQFQGPVPAKSHPLSFSYQLKLANDVSPT 60

Query: 61  NDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120
            DHR SF QTAAVRHIVGS++KA GLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED
Sbjct: 61  KDHRCSFVQTAAVRHIVGSIAKAGGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSVQDED 120

Query: 121 IDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSAGLKS 180
           IDVVWVPGSFDIPVVAERLG SGKYHA+LCIGAVI+GDTSHYDAVV+S+ASGVLSAGLKS
Sbjct: 121 IDVVWVPGSFDIPVVAERLGTSGKYHAVLCIGAVIRGDTSHYDAVVSSSASGVLSAGLKS 180

Query: 181 GVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
           GVPCVFSVLTCDNLDQAFNRAGGK GNKGSEGALTA+
Sbjct: 181 GVPCVFSVLTCDNLDQAFNRAGGKHGNKGSEGALTAM 217

[Close]

BLAST of MS000997.1 vs. TAIR 10
Match: AT2G44050.1 (6,7-dimethyl-8-ribityllumazine synthase / DMRL synthase / lumazine synthase / riboflavin synthase )

HSP 1 Score: 245.7 bits (626), Expect = 3.3e-65
Identity = 131/221 (59.28%), Postives = 164/221 (74.21%), Query Frame = 0


Query: 1   MAASFASPAQGLLHRQHLPLAFTNSNFNRTQLQFHGATPA-KSQPLSFSSQLKLSNDASP 60
           M +  + P   L+   H  L   NS  + +    HG +   KS  LSFSS    S  ASP
Sbjct: 1   MKSLASPPCLRLIPTAHRQL---NSRQSSSACYIHGGSSVNKSNNLSFSS--STSGFASP 60

Query: 61  ---RNDHRSSFAQTAAVRHIVGSLSKAEGLRFAVVVGRFNEIVTRPMLEGALSTFKSYSV 120
                + RSSF QTAAVRH+ GSL + EGLRFA+VV RFNE+VT+ +LEGA+ TFK YSV
Sbjct: 61  LAVEKELRSSFVQTAAVRHVTGSLIRGEGLRFAIVVARFNEVVTKLLLEGAIETFKKYSV 120

Query: 121 QDEDIDVVWVPGSFDIPVVAERLGKSGKYHAILCIGAVIKGDTSHYDAVVNSTASGVLSA 180
           ++EDI+V+WVPGSF+I VVA+ LGKSGK+HA+LCIGAVI+GDT+HYDAV NS ASGVLSA
Sbjct: 121 REEDIEVIWVPGSFEIGVVAQNLGKSGKFHAVLCIGAVIRGDTTHYDAVANSAASGVLSA 180

Query: 181 GLKSGVPCVFSVLTCDNLDQAFNRAGGKSGNKGSEGALTAV 218
            + SGVPC+F VLTC+++DQA NR+GGK+GNKG+E ALTA+
Sbjct: 181 SINSGVPCIFGVLTCEDMDQALNRSGGKAGNKGAETALTAL 216

The following BLAST results are available for this feature:

BLAST of MS000997.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132287.1	1.8e-113	98.16	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic [Momordica charantia] >AF...	[more]
XP_038884169.1	2.7e-101	88.02	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic [Benincasa hispida]	[more]
XP_023002869.1	1.3e-98	86.64	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic-like [Cucurbita maxima]	[more]
XP_022951341.1	1.3e-98	86.64	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic-like [Cucurbita moschata]...	[more]
XP_023537234.1	3.6e-98	86.18	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic-like [Cucurbita pepo subs...	[more]

BLAST of MS000997.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80575	4.7e-64	59.28	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic OS=Arabidopsis thaliana O...	[more]
Q9XH32	4.5e-59	66.48	6,7-dimethyl-8-ribityllumazine synthase, chloroplastic OS=Spinacia oleracea OX=3...	[more]
A6LSS9	7.5e-38	51.11	6,7-dimethyl-8-ribityllumazine synthase OS=Clostridium beijerinckii (strain ATCC...	[more]
A5D1C7	1.3e-37	48.95	6,7-dimethyl-8-ribityllumazine synthase OS=Pelotomaculum thermopropionicum (stra...	[more]
B2TJ82	1.4e-36	51.11	6,7-dimethyl-8-ribityllumazine synthase OS=Clostridium botulinum (strain Eklund ...	[more]

BLAST of MS000997.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
L0C9N6	8.7e-114	98.16	6,7-dimethyl-8-ribityllumazine synthase OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1KRP6	6.1e-99	86.64	6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita maxima OX=3661 GN=LOC111496...	[more]
A0A6J1GHB3	6.1e-99	86.64	6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KDC4	2.5e-97	84.79	6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A6J1HD10	7.4e-97	84.79	6,7-dimethyl-8-ribityllumazine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of MS000997.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44050.1	3.3e-65	59.28	6,7-dimethyl-8-ribityllumazine synthase / DMRL synthase / lumazine synthase / ri...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036467	Lumazine/riboflavin synthase superfamily	GENE3D	3.40.50.960	Lumazine/riboflavin synthase	coord: 73..217 e-value: 1.9E-58 score: 198.4
IPR036467	Lumazine/riboflavin synthase superfamily	SUPERFAMILY	52121	Lumazine synthase	coord: 78..217
IPR034964	Lumazine synthase	TIGRFAM	TIGR00114	TIGR00114	coord: 86..217 e-value: 8.7E-40 score: 134.0
IPR034964	Lumazine synthase	PANTHER	PTHR21058	6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE	coord: 21..217
IPR034964	Lumazine synthase	HAMAP	MF_00178	Lumazine_synth	coord: 81..217 score: 22.909485
IPR034964	Lumazine synthase	CDD	cd09209	Lumazine_synthase-I	coord: 88..217 e-value: 2.03406E-68 score: 204.216
IPR002180	Lumazine/riboflavin synthase	PFAM	PF00885	DMRL_synthase	coord: 85..217 e-value: 2.2E-44 score: 150.7
None	No IPR available	PANTHER	PTHR21058:SF1	6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE	coord: 21..217

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS000997	MS000997	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS000997.1-cds	MS000997.1-cds-scaffold36:729843..730112	CDS
MS000997.1-cds	MS000997.1-cds-scaffold36:730781..730876	CDS
MS000997.1-cds	MS000997.1-cds-scaffold36:731191..731286	CDS
MS000997.1-cds	MS000997.1-cds-scaffold36:731376..731454	CDS
MS000997.1-cds	MS000997.1-cds-scaffold36:732383..732429	CDS
MS000997.1-cds	MS000997.1-cds-scaffold36:732538..732600	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS000997.1	MS000997.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009231	riboflavin biosynthetic process
cellular_component	GO:0009349	riboflavin synthase complex
molecular_function	GO:0000906	6,7-dimethyl-8-ribityllumazine synthase activity