MS000882.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS000882.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	dual-specificity RNA methyltransferase RlmN
Location	scaffold36: 50114 .. 53000 (-)
Sequence length	1434
RNA-Seq Expression	MS000882.1
Synteny	MS000882.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTGTTTGGATGAGACGCCTGTTCTTCCCTAAGGCTTCCATGGCCTCCTCTATCCTTGCCGTTGCCCGATCGCCTTTCCGTCCCCTTTCCGTCCCTTCCTCACGCCTATCTCTACCATTTCACCTAATTTCATTCGTTTTTTCTGCTAGTCGCCCCATTTCAACTGCCTCTTGCGCCTCACCCACGCCGCTATTTGCTCTTGACGATTCTTCATCTGATTTCTGTCAGTTCCCGAATATCTTTGGCTGTATACTCACATTCTTTTACTCTATTGTTTGACTACTTATCTTCCCTTTCCTCTGCTTCATTTAACTATGTTTTTCAATTCGGTTTATTTTGTTACCATTTACATCCGAATCATACTTTTGCGGACTCTTTTCTTATAGTTGTCATCTTGTAGTTATGTCTGACAAGAGGGAAATCCTGAAGAATGATTCCAAGGTGCTTCTAAAGGGACTCTCTTACACTGAGCTTGAGGCAAGTACCTGCTAAACCCTCTCTCTCTCTCGTCTGCAATTATTTGTCTCTCCTTCCGCGCTTAATAAAGAAAAGCTTTTGCTCTTACACGCGCACTCAATTTTTTTTATTGTAACATATACGTCAATAGAAATGGGTTCAATCACGAGGATACAGACCTGGCCAAGCTTTAATGCTGTGGAAACGTCTTTATGGAGATAACATTTGGGCACATACCGGCGATGAATTAGAAGGTGTTCTTGCTTCCTCAGTGTATTTGGAATACGAGCCTTTCTTTACATTTTAACATTTTTATTGGTTGGCAGAATTTTTCCTTCATCTCCTTTCCTCTTGTTGTAACTTGGAACTTATCTACTCAATCTAATGGATTTTCTAATGCTGTCAAATTCGTAGGTTTAAACAAATATTTTAAGAAAATGTTGATTGAAAACGCTGAATTCAGGGCGCTGTCATTGAGGGAAATTCTCCCTTCATCTGATGGAACTAGGAAGGTGTGTATTGTTACATCTGATGGAACTATTATAATCATTCAGTTCTCTTTGCTTCTAGTTTCTGTATTGGTTCCGGCTCCACTGAGCAAAATCATTAGGTTTGGCTTTAGTTCTTTGGATTTGTGCTCTCAAGATTCATCTGATTAATTTAAAGCTATCAGGACATTTCAGATTTTGTTCGATCTGGAAGATGGATTGATAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACTGTTTGCGTTTCAAGTCAAGTGGGCTGTGCCATGAACTGTCAATTCTGCTACACTGGCAGGCAAGCTTCAATTTTTCAAATGGATAGATGCTTTCGGCATGATCTTGGTGGTACTTGTAAACGAGAATGTTGATTTAATTTTTCAGGATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGCCAGGCGTTTACTTACTAGTGAAGTGGGTTCTATTACTAATGTCGTGTTTATGGTATGTTTTACATCTGAAACTACTTGACATACAAGAGGGAAAATGAAATATTATTGAAAATTCTTGAGTCTCTTTTGGGGCGTTTGCTATTTCTTTTGACATGCTGTTCTACCCTATATTCAGTTCGTGGTTTATTAACTAAGCTCGCTTTAGGAAATAATCCAAAAGGATAGGATTTGGTTAGAAGTAACATCTAATTGAGGGTGGTCCTAGTTGGTTTGACGCTCTTATAGGAGTATGATACTTTCTTTCATTCAAGCTGTGCTTTAGTTTGAAGTGTGCGCAACATTCTTTCTTTTTTATCATTGTTGTTGACTTGATGCTGTTATTTTCCAGCCTATTCCAAGCCACAAGTCCTTTATGCTTAGCAGGGACAAAATTTGCAGCCATTTATTGCGTTCTTAGTTTTCTTCCCTGATAAATTTCTATTGATTTCTTCGTTTTTCAGTCATATTGAAGACTTATGTATTTTTGCCTTTTCTCTTCATTGTATCGACTGTTAAAACCTTTTGAGTTTTGACATTTATAATGGTGGTATCAAGCCATTATCCTTTTCCATGTTTTACTTAGAAAAACTGAAATTGAATGTTAGGGAATGGGAGAGCCACTTCACAACATTGACAATGTCACTAAAGCAGCAAACATAATGGTTCATGAACAAGGCCTTCATTTCAGTCCCCGCAAGGTCACTGTCTCAACCAGTGGACTTGTACCTCAGCTAAAACGTTTCCTTCGTGAATGTGACTGTGCTTTAGCTGTTAGTTTGAATGCAACTACTGATGAGGTACATGCTTAACGGTTGGCATAACTCAAATGTGCAAAAAAGAAAAAATATGAAGAATTGCTTTCTCTTACATGGAAAAATGTTGCAGGTTAGGAATTGGATCATGCCAATTAACAGGAAATATAAGTTAGGCTTGCTTCTTCAGACTTTAAGGGAGGAACTTTGCTCGAAACACAATTACAAGGTTTTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACGGGTATATTACAACTTCTATGTTTTGGACTTAATCTGGGTGTGTACTTGATGAGTTTATCAACTTTATCTTTACAGTCCTTTCTAATCTTTTGTTTTGACATACTAATTAGTTGTATTTTTATTTGCAGCCTGGAAGATGCGAAGAGGCTGGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAATCTTATTTCATTTAATCCACATAGTGGATCTCAGTTCAGACCTACCCGCAGGGAGAAGATGATTGAGTTTCGGAATGTTTTGGCTGAAGCTGGGTTGACTGTTTTCTTGCGACTAAGTAGAGGCGACGACCAGATGGCTGCCTGTGGTCAGTTGGGCAAACCTGGTACTATTCAAGCTCCTTTACTCCGTGTTCCAGAACAATTTCAAATGGCAATGAAATTGGCTCTC

mRNA sequence

CGTGTTTGGATGAGACGCCTGTTCTTCCCTAAGGCTTCCATGGCCTCCTCTATCCTTGCCGTTGCCCGATCGCCTTTCCGTCCCCTTTCCGTCCCTTCCTCACGCCTATCTCTACCATTTCACCTAATTTCATTCGTTTTTTCTGCTAGTCGCCCCATTTCAACTGCCTCTTGCGCCTCACCCACGCCGCTATTTGCTCTTGACGATTCTTCATCTGATTTCTGTCATTGTCATCTTGTAGTTATGTCTGACAAGAGGGAAATCCTGAAGAATGATTCCAAGGTGCTTCTAAAGGGACTCTCTTACACTGAGCTTGAGGCAAGATACAGACCTGGCCAAGCTTTAATGCTGTGGAAACGTCTTTATGGAGATAACATTTGGGCACATACCGGCGATGAATTAGAAGGTTTAAACAAATATTTTAAGAAAATGTTGATTGAAAACGCTGAATTCAGGGCGCTGTCATTGAGGGAAATTCTCCCTTCATCTGATGGAACTAGGAAGGTGTTTCTTTGGATTTGTGCTCTCAAGATTCATCTGATTTTTAAAGCTATCAGGACATTTCAGATTTTGTTCGATCTGGAAGATGGATTGATAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACTGTTTGCGTTTCAAGTCAAGTGGGCTGTGCCATGAACTGTCAATTCTGCTACACTGGCAGGCAAGCTTCAATTATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGCCAGGCGTTTACTTACTAGTGAAGTGGGTTCTATTACTAATGTCGTGTTTATGGGAATGGGAGAGCCACTTCACAACATTGACAATGTCACTAAAGCAGCAAACATAATGGTTCATGAACAAGGCCTTCATTTCAGTCCCCGCAAGGTCACTGTCTCAACCAGTGGACTTGTACCTCAGCTAAAACGTTTCCTTCGTGAATGTGACTGTGCTTTAGCTGTTAGTTTGAATGCAACTACTGATGAGGTTAGGAATTGGATCATGCCAATTAACAGGAAATATAAGTTAGGCTTGCTTCTTCAGACTTTAAGGGAGGAACTTTGCTCGAAACACAATTACAAGGTTTTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACGGCCTGGAAGATGCGAAGAGGCTGGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAATCTTATTTCATTTAATCCACATAGTGGATCTCAGTTCAGACCTACCCGCAGGGAGAAGATGATTGAGTTTCGGAATGTTTTGGCTGAAGCTGGGTTGACTGTTTTCTTGCGACTAAGTAGAGGCGACGACCAGATGGCTGCCTGTGGTCAGTTGGGCAAACCTGGTACTATTCAAGCTCCTTTACTCCGTGTTCCAGAACAATTTCAAATGGCAATGAAATTGGCTCTC

Coding sequence (CDS)

CGTGTTTGGATGAGACGCCTGTTCTTCCCTAAGGCTTCCATGGCCTCCTCTATCCTTGCCGTTGCCCGATCGCCTTTCCGTCCCCTTTCCGTCCCTTCCTCACGCCTATCTCTACCATTTCACCTAATTTCATTCGTTTTTTCTGCTAGTCGCCCCATTTCAACTGCCTCTTGCGCCTCACCCACGCCGCTATTTGCTCTTGACGATTCTTCATCTGATTTCTGTCATTGTCATCTTGTAGTTATGTCTGACAAGAGGGAAATCCTGAAGAATGATTCCAAGGTGCTTCTAAAGGGACTCTCTTACACTGAGCTTGAGGCAAGATACAGACCTGGCCAAGCTTTAATGCTGTGGAAACGTCTTTATGGAGATAACATTTGGGCACATACCGGCGATGAATTAGAAGGTTTAAACAAATATTTTAAGAAAATGTTGATTGAAAACGCTGAATTCAGGGCGCTGTCATTGAGGGAAATTCTCCCTTCATCTGATGGAACTAGGAAGGTGTTTCTTTGGATTTGTGCTCTCAAGATTCATCTGATTTTTAAAGCTATCAGGACATTTCAGATTTTGTTCGATCTGGAAGATGGATTGATAATAGAAACTGTTGTTATACCTTGCGATAGAGGCAGGACTACTGTTTGCGTTTCAAGTCAAGTGGGCTGTGCCATGAACTGTCAATTCTGCTACACTGGCAGGCAAGCTTCAATTATGGGCCTGAAGAGACATCTGACTGCTGCTGAGATAGTAGAACAGGCAGTTTTTGCCAGGCGTTTACTTACTAGTGAAGTGGGTTCTATTACTAATGTCGTGTTTATGGGAATGGGAGAGCCACTTCACAACATTGACAATGTCACTAAAGCAGCAAACATAATGGTTCATGAACAAGGCCTTCATTTCAGTCCCCGCAAGGTCACTGTCTCAACCAGTGGACTTGTACCTCAGCTAAAACGTTTCCTTCGTGAATGTGACTGTGCTTTAGCTGTTAGTTTGAATGCAACTACTGATGAGGTTAGGAATTGGATCATGCCAATTAACAGGAAATATAAGTTAGGCTTGCTTCTTCAGACTTTAAGGGAGGAACTTTGCTCGAAACACAATTACAAGGTTTTTTTTGAATATGTGATGCTTGCTGGGGTTAATGACGGCCTGGAAGATGCGAAGAGGCTGGTTGATCTTGTCCAGGGTATTCCATGCAAGATTAATCTTATTTCATTTAATCCACATAGTGGATCTCAGTTCAGACCTACCCGCAGGGAGAAGATGATTGAGTTTCGGAATGTTTTGGCTGAAGCTGGGTTGACTGTTTTCTTGCGACTAAGTAGAGGCGACGACCAGATGGCTGCCTGTGGTCAGTTGGGCAAACCTGGTACTATTCAAGCTCCTTTACTCCGTGTTCCAGAACAATTTCAAATGGCAATGAAATTGGCTCTC

Protein sequence

RVWMRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTPLFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELEARYRPGQALMLWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKIHLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLAL

Homology

[Close]

BLAST of MS000882.1 vs. NCBI nr
Match: XP_022159137.1 (uncharacterized protein LOC111025565 isoform X1 [Momordica charantia])

HSP 1 Score: 844.0 bits (2179), Expect = 6.5e-241
Identity = 441/482 (91.49%), Postives = 442/482 (91.70%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPKASMASSILAVARS FRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP
Sbjct: 1   MRRLFFPKASMASSILAVARSHFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 60

Query: 64  LFALDDSSSDFCHCHLV--VMSDKREILKNDSKVLLKGLSYTELE-----ARYRPGQALM 123
           LFALDDSSSDFC    +   MSDKREILKNDSKVLLKGLSYTELE       YRPGQALM
Sbjct: 61  LFALDDSSSDFCQFPNIFGFMSDKREILKNDSKVLLKGLSYTELEKWVQSRGYRPGQALM 120

Query: 124 LWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICAL 183
           LWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK        
Sbjct: 121 LWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK-------- 180

Query: 184 KIHLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQAS 243
                        ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR   
Sbjct: 181 -------------ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR--- 240

Query: 244 IMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQ 303
            MGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQ
Sbjct: 241 -MGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQ 300

Query: 304 GLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQ 363
           GLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQ
Sbjct: 301 GLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQ 360

Query: 364 TLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRP 423
           TLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRP
Sbjct: 361 TLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRP 420

Query: 424 TRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKL 479
           TRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKL
Sbjct: 421 TRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKL 457

[Close]

BLAST of MS000882.1 vs. NCBI nr
Match: XP_022159138.1 (uncharacterized protein LOC111025565 isoform X2 [Momordica charantia])

HSP 1 Score: 840.5 bits (2170), Expect = 7.2e-240
Identity = 440/480 (91.67%), Postives = 440/480 (91.67%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPKASMASSILAVARS FRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP
Sbjct: 1   MRRLFFPKASMASSILAVARSHFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 60

Query: 64  LFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE-----ARYRPGQALMLW 123
           LFALDDSSSDF       MSDKREILKNDSKVLLKGLSYTELE       YRPGQALMLW
Sbjct: 61  LFALDDSSSDF------FMSDKREILKNDSKVLLKGLSYTELEKWVQSRGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKI 183
           KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK---------- 180

Query: 184 HLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIM 243
                      ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    M
Sbjct: 181 -----------ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----M 240

Query: 244 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 303
           GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL
Sbjct: 241 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 300

Query: 304 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL
Sbjct: 301 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 360

Query: 364 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 423
           REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR
Sbjct: 361 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 420

Query: 424 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLAL 479
           REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLAL
Sbjct: 421 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLAL 449

[Close]

BLAST of MS000882.1 vs. NCBI nr
Match: XP_023546952.1 (uncharacterized protein LOC111805899 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 734.9 bits (1896), Expect = 4.3e-208
Identity = 392/479 (81.84%), Postives = 408/479 (85.18%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPK SMASSIL  A S F  LS+PS+RL  P HLISF+ SA R  +TAS ASPTP
Sbjct: 1   MRRLFFPKPSMASSIL--APSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPTP 60

Query: 64  LFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE----AR-YRPGQALMLW 123
           LFALDDSS DF       M +K+EIL  DSK+LLKGLSYTELE    AR YRPGQALMLW
Sbjct: 61  LFALDDSSPDFS------MPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKI 183
           KRLYGDN+WAH+GDELEGLNK FKKMLIE AEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK---------- 180

Query: 184 HLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIM 243
                      ILF+LEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    M
Sbjct: 181 -----------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----M 240

Query: 244 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 303
           GLKRHLTAAEIVEQAVFARRLLT EVG ITNVVFMGMGEPLHNIDNV KAANIMV EQGL
Sbjct: 241 GLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGL 300

Query: 304 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFL +C+CALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL
Sbjct: 301 HFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 360

Query: 364 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 423
           REEL  KHNYKV FEYVMLAGVND +EDAKR+VDLVQGIPCKINLISFNPH GSQFRPT 
Sbjct: 361 REELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTC 420

Query: 424 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLA 478
           +EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGTIQAPLLRVP+QFQMAMKLA
Sbjct: 421 KEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLA 446

[Close]

BLAST of MS000882.1 vs. NCBI nr
Match: XP_022961770.1 (uncharacterized protein LOC111462441 isoform X1 [Cucurbita moschata])

HSP 1 Score: 733.8 bits (1893), Expect = 9.5e-208
Identity = 391/479 (81.63%), Postives = 408/479 (85.18%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPK SMASSIL  A S F  LS+PS+RL  P HLISF+ SA R  +TAS ASPTP
Sbjct: 1   MRRLFFPKPSMASSIL--APSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPTP 60

Query: 64  LFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE----AR-YRPGQALMLW 123
           LFALDDSS DF       M +K+EIL  DSK+LLKGLSYTELE    AR YRPGQALMLW
Sbjct: 61  LFALDDSSPDFS------MPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKI 183
           KRLYGDN+WAH+GDELEGLNK FKKMLIE AEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK---------- 180

Query: 184 HLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIM 243
                      ILF+LEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    M
Sbjct: 181 -----------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----M 240

Query: 244 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 303
           GLKRHLTAAEIVEQAVFARRLLT +VG ITNVVFMGMGEPLHNIDNV KAANIMV EQGL
Sbjct: 241 GLKRHLTAAEIVEQAVFARRLLTCDVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGL 300

Query: 304 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFL +C+CALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL
Sbjct: 301 HFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 360

Query: 364 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 423
           REEL  KHNYKV FEYVMLAGVND +EDAKR+VDLVQGIPCKINLISFNPH GSQFRPT 
Sbjct: 361 REELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTC 420

Query: 424 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLA 478
           +EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGTIQAPLLRVP+QFQMAMKLA
Sbjct: 421 KEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLA 446

[Close]

BLAST of MS000882.1 vs. NCBI nr
Match: XP_022996535.1 (uncharacterized protein LOC111491756 isoform X1 [Cucurbita maxima])

HSP 1 Score: 732.6 bits (1890), Expect = 2.1e-207
Identity = 391/479 (81.63%), Postives = 407/479 (84.97%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPK SMASSIL  A S F  LS+PS+RL  P HLISF+ SA R  +TAS ASP P
Sbjct: 1   MRRLFFPKPSMASSIL--APSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPKP 60

Query: 64  LFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE----AR-YRPGQALMLW 123
           LFALDDSS DF       M +K+EIL  DSK+LLKGLSYTELE    AR YRPGQALMLW
Sbjct: 61  LFALDDSSPDFS------MPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKI 183
           KRLYGDN+WAH+GDELEGLNK FKKMLIE AEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK---------- 180

Query: 184 HLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIM 243
                      ILF+LEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    M
Sbjct: 181 -----------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----M 240

Query: 244 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 303
           GLKRHLTAAEIVEQAVFARRLLT EVG ITNVVFMGMGEPLHNIDNV KAANIMV EQGL
Sbjct: 241 GLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGL 300

Query: 304 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFL +C+CALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL
Sbjct: 301 HFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 360

Query: 364 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 423
           REEL  KHNYKV FEYVMLAGVND +EDAKR+VDLVQGIPCKINLISFNPH GSQFRPT 
Sbjct: 361 REELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTC 420

Query: 424 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLA 478
           +EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGTIQAPLLRVP+QFQMAMKLA
Sbjct: 421 KEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLA 446

[Close]

BLAST of MS000882.1 vs. ExPASy Swiss-Prot
Match: Q74E53 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=rlmN PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 9.6e-70
Identity = 167/370 (45.14%), Postives = 211/370 (57.03%), Query Frame = 0


Query: 92  DSKVLLKGLSYTELE--------ARYRPGQALMLWKRLYGDNIWAHTGDELEGLNKYFKK 151
           D+ + +KGLS  ELE         RYR   A  ++K LY     A +  E+  L K  ++
Sbjct: 2   DTMIDIKGLSIDELERFLLGKGKERYR---ARQIFKWLYQRG--ATSFAEMTDLAKELRR 61

Query: 152 MLIENAEFRALSLREILPSSDGTRKVFLWICALKIHLIFKAIRTFQILFDLEDGLIIETV 211
            L E A    LS   +  S DGTRK                      LF L+DG  +E+V
Sbjct: 62  DLEETARISTLSPEALEISRDGTRK---------------------YLFRLDDGCSVESV 121

Query: 212 VIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHLTAAEIVEQAVFARRLLTSE 271
           +IP +  R T+C+SSQVGCAM C+FC TG       L R+LT AEIV Q    +R +   
Sbjct: 122 LIP-EEDRNTLCISSQVGCAMACEFCLTG----TFRLTRNLTTAEIVNQVCAVQRDV--- 181

Query: 272 VGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLREC 331
              + N+VFMGMGEPL N+DNV +A  IM+H+ GL FS R++TVST+GLVP+++R  R  
Sbjct: 182 --PVRNIVFMGMGEPLANLDNVIRALQIMLHDDGLQFSTRRITVSTAGLVPEMERLGRSV 241

Query: 332 DCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELCSKHNYKVFFEYVMLAGVNDG 391
              LAVSLNATTDE+R+ IMPINRKY L +LL   R         K+  EYV+L GVND 
Sbjct: 242 TVNLAVSLNATTDELRDRIMPINRKYPLAVLLDACR-RFPLPGRRKITIEYVLLGGVNDT 301

Query: 392 LEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTRREKMIEFRNVLAEAGLTVFLRLSRG 451
           L+DAKRLV L+  IP KINLI FN H G  FR   ++ +  F   L +   TV  R SRG
Sbjct: 302 LDDAKRLVRLLSDIPSKINLIPFNEHEGCSFRSPSQDAIDRFHRYLLDKHFTVITRSSRG 334

Query: 452 DDQMAACGQL 454
            D  AACGQL
Sbjct: 362 ADISAACGQL 334

[Close]

BLAST of MS000882.1 vs. ExPASy Swiss-Prot
Match: Q3A2Z4 (Dual-specificity RNA methyltransferase RlmN OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=rlmN PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-68
Identity = 158/384 (41.15%), Postives = 214/384 (55.73%), Query Frame = 0


Query: 89  LKNDSKVLLKGLSYTEL--------EARYRPGQAL-MLWKRLYGDNIWAHTGDELEGLNK 148
           +  D+++ LK  +  EL        + R+R GQ +  ++ RL  D       D +  L+K
Sbjct: 5   MDQDTRIDLKNFTLEELTEFLAGMGKERFRAGQVMRWMYHRLVDD------FDAMSDLSK 64

Query: 149 YFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKIHLIFKAIRTFQILFDLEDGLI 208
             +  L + A    L+      S DGTRK                      LF LEDG  
Sbjct: 65  VLRAELHQRARISRLTPEATEDSRDGTRK---------------------YLFRLEDGET 124

Query: 209 IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHLTAAEIVEQAVFARRL 268
           IE+V IP D  R T+C+S+QVGCAM C FC+TG      GL R+LT  EIV Q   A   
Sbjct: 125 IESVRIPMDDNRATLCISTQVGCAMGCVFCHTGS----FGLVRNLTPGEIVNQVCAA--- 184

Query: 269 LTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRF 328
                G + N+V MGMGEPLHN+DNV KA  I+   QGL +SPRKVT+ST+GLVPQ++  
Sbjct: 185 --LADGPVNNIVLMGMGEPLHNLDNVVKALQILYMPQGLDYSPRKVTLSTAGLVPQMQEL 244

Query: 329 LRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLRE-ELCSKHNYKVFFEYVMLA 388
            +     LAVSLNATTDEVRN +MP+N++Y L  L+   R+  L +K   ++ FEY+++ 
Sbjct: 245 GKRVRVNLAVSLNATTDEVRNRLMPVNQRYPLQQLMAACRQYPLHAKK--RITFEYILIR 304

Query: 389 GVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTRREKMIEFRNVLAEAGLTVFL 448
            VND  +DA+RLV L+ GI  K+N+I FN HS S+FR    E++  F+  L + G+    
Sbjct: 305 DVNDSDQDARRLVKLLHGIKAKVNIIPFNEHSASEFRAPTEERISRFQGYLLDHGMVAIR 350

Query: 449 RLSRGDDQMAACGQLGKPGTIQAP 463
           R S+G D  AACGQL    T+  P
Sbjct: 365 RASKGQDISAACGQLKGKLTVSPP 350

[Close]

BLAST of MS000882.1 vs. ExPASy Swiss-Prot
Match: Q39S71 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=rlmN PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.1e-68
Identity = 167/370 (45.14%), Postives = 210/370 (56.76%), Query Frame = 0


Query: 92  DSKVLLKGLSYTELEA--------RYRPGQALMLWKRLYGDNIWAHTGDELEGLNKYFKK 151
           + K+ +K L+  +L A        RYR   A  ++K LY  +  A +  E+  L K  ++
Sbjct: 3   NGKIDIKNLTLDDLIAFLAGKGKERYR---ARQIFKWLYQKD--ARSFAEMTDLAKDLRR 62

Query: 152 MLIENAEFRALSLREILPSSDGTRKVFLWICALKIHLIFKAIRTFQILFDLEDGLIIETV 211
            L E A    L    +  S DGTRK                      LF LEDG  +E+V
Sbjct: 63  DLEETAVISDLEPEAMEVSRDGTRK---------------------YLFRLEDGNTVESV 122

Query: 212 VIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHLTAAEIVEQAVFARRLLTSE 271
           +IP +  RTT+C+SSQVGCAM C+FC TG       L R+LTA EIV Q    RR +   
Sbjct: 123 LIP-EEDRTTLCISSQVGCAMACEFCLTG----TFRLTRNLTAGEIVNQICAVRRDV--- 182

Query: 272 VGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLREC 331
              + N+VFMGMGEPL N+DNV KA  I++H+ GL FS R+VTVSTSGLVP+++R  RE 
Sbjct: 183 --PVRNIVFMGMGEPLANLDNVVKALKIILHDDGLQFSTRRVTVSTSGLVPEMERLGREV 242

Query: 332 DCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELCSKHNYKVFFEYVMLAGVNDG 391
              LAVSLNATTDEVR+ IMP+NR+Y L LLL   R         K+  EYVM+ G+ND 
Sbjct: 243 TVNLAVSLNATTDEVRDRIMPVNRRYPLRLLLDACRSYPLPGRR-KITIEYVMIKGLNDS 302

Query: 392 LEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTRREKMIEFRNVLAEAGLTVFLRLSRG 451
           LEDAKRLV L+  I  KINLI FN H G  F+   +  +  F + L     TV  R SRG
Sbjct: 303 LEDAKRLVKLLSDISSKINLIPFNEHDGCSFKSPDQGAIDVFHSYLLSKHFTVITRSSRG 335

Query: 452 DDQMAACGQL 454
            D  AACGQL
Sbjct: 363 SDISAACGQL 335

[Close]

BLAST of MS000882.1 vs. ExPASy Swiss-Prot
Match: A0LQM1 (Dual-specificity RNA methyltransferase RlmN OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=rlmN PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.2e-65
Identity = 145/344 (42.15%), Postives = 204/344 (59.30%), Query Frame = 0


Query: 110 RPGQALMLWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKV 169
           R  +A  L++ +YG  +   +  E   L++ F+  L    E  ALS+ +   + DGT K 
Sbjct: 24  RSFRARQLFRHVYGRGV--RSWSECSDLSRMFRVQLEHGVELDALSVLKKEQADDGTSK- 83

Query: 170 FLWICALKIHLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFC 229
                                LF L DG  IE V+IP D  R+T+CVSSQVGCA+ C+FC
Sbjct: 84  --------------------YLFGLRDGHSIEAVLIP-DLPRSTLCVSSQVGCALGCKFC 143

Query: 230 YTGRQASIMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAA 289
            TG     +G KR+L+AAEIV+Q    +R L S    ITN+VFMGMGEPL N+D+V +A 
Sbjct: 144 LTGS----LGFKRNLSAAEIVDQVCQVQRDLGSR-SRITNIVFMGMGEPLANLDSVLRAI 203

Query: 290 NIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKY 349
            ++    G+ FS R++T+ST+GLVPQL+R  RE    LAVSL+A  +E+R  +MP+NR Y
Sbjct: 204 RVIAEPNGMAFSHRRITLSTAGLVPQLRRLGRESPVNLAVSLHAAENELRAELMPVNRTY 263

Query: 350 KLGLLLQTLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPH 409
            L +L+   RE        ++ FEY++L G+ND  + AK+LV L+ GI  K+NL+ FNPH
Sbjct: 264 PLEVLMAACREYPLPPRK-RITFEYILLDGINDDPKQAKQLVKLLHGIRAKVNLMPFNPH 323

Query: 410 SGSQFRPTRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQL 454
            GS FR    ++++ F+  L  A +T  +R SRG +  AACGQL
Sbjct: 324 PGSVFRKPSEQRVLAFQEALQNARITTHVRRSRGGEIGAACGQL 337

[Close]

BLAST of MS000882.1 vs. ExPASy Swiss-Prot
Match: A5GEC2 (Dual-specificity RNA methyltransferase RlmN OS=Geobacter uraniireducens (strain Rf4) OX=351605 GN=rlmN PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.1e-65
Identity = 158/366 (43.17%), Postives = 202/366 (55.19%), Query Frame = 0


Query: 94  KVLLKGLSYTELEARYRPGQ------ALMLWKRLYGDNIWAHTGDELEGLNKYFKKMLIE 153
           KV +K  +  ELEA Y  GQ      A  ++K LY  +  A    ++  L+K F++ L +
Sbjct: 3   KVDIKNFTLQELEA-YIAGQGKERFRAKQIFKWLYQQD--AREFADMTNLSKDFRQELEK 62

Query: 154 NAEFRALSLREILPSSDGTRKVFLWICALKIHLIFKAIRTFQILFDLEDGLIIETVVIPC 213
            A    L    +  S+DGT+K                      LF L DG  +E+V+IP 
Sbjct: 63  TAWISNLDAEAVEASADGTKK---------------------YLFRLADGNAVESVLIP- 122

Query: 214 DRGRTTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHLTAAEIVEQAVFARRLLTSEVGSI 273
           D  RTT+C+SSQVGCAM C+FC TG       L R+LT AEIV Q    +R        +
Sbjct: 123 DEDRTTLCISSQVGCAMGCEFCLTG----TFKLTRNLTTAEIVNQVCAVKR-----QEPV 182

Query: 274 TNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLRECDCAL 333
            N+VFMGMGEPL N+ NV  A  I+    G  FS RKVTVSTSGLVP++          L
Sbjct: 183 RNIVFMGMGEPLANLKNVVGALKILTDPDGFQFSTRKVTVSTSGLVPEMAELGASVTVNL 242

Query: 334 AVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELCSKHNYKVFFEYVMLAGVNDGLEDA 393
           AVSLNATTDEVR+ IMPINR+Y L  LL   +        + +  EYVM+ GVND L+DA
Sbjct: 243 AVSLNATTDEVRDRIMPINRRYPLKELLAACKAFPLPSRRW-ITIEYVMIRGVNDSLDDA 302

Query: 394 KRLVDLVQGIPCKINLISFNPHSGSQFRPTRREKMIEFRNVLAEAGLTVFLRLSRGDDQM 453
           KRLV L+  IP K+NLI FN H G  F+   ++ + +F   L +  +TV  R SRG D  
Sbjct: 303 KRLVRLISNIPSKVNLIPFNEHDGCTFQAPTQDSIDKFHKFLLDKHVTVITRSSRGSDIS 333

[Close]

BLAST of MS000882.1 vs. ExPASy TrEMBL
Match: A0A6J1DYZ7 (uncharacterized protein LOC111025565 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025565 PE=4 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.2e-241
Identity = 441/482 (91.49%), Postives = 442/482 (91.70%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPKASMASSILAVARS FRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP
Sbjct: 1   MRRLFFPKASMASSILAVARSHFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 60

Query: 64  LFALDDSSSDFCHCHLV--VMSDKREILKNDSKVLLKGLSYTELE-----ARYRPGQALM 123
           LFALDDSSSDFC    +   MSDKREILKNDSKVLLKGLSYTELE       YRPGQALM
Sbjct: 61  LFALDDSSSDFCQFPNIFGFMSDKREILKNDSKVLLKGLSYTELEKWVQSRGYRPGQALM 120

Query: 124 LWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICAL 183
           LWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK        
Sbjct: 121 LWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK-------- 180

Query: 184 KIHLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQAS 243
                        ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR   
Sbjct: 181 -------------ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR--- 240

Query: 244 IMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQ 303
            MGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQ
Sbjct: 241 -MGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQ 300

Query: 304 GLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQ 363
           GLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQ
Sbjct: 301 GLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQ 360

Query: 364 TLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRP 423
           TLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRP
Sbjct: 361 TLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRP 420

Query: 424 TRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKL 479
           TRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKL
Sbjct: 421 TRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKL 457

[Close]

BLAST of MS000882.1 vs. ExPASy TrEMBL
Match: A0A6J1DXZ7 (uncharacterized protein LOC111025565 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025565 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.5e-240
Identity = 440/480 (91.67%), Postives = 440/480 (91.67%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPKASMASSILAVARS FRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP
Sbjct: 1   MRRLFFPKASMASSILAVARSHFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 60

Query: 64  LFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE-----ARYRPGQALMLW 123
           LFALDDSSSDF       MSDKREILKNDSKVLLKGLSYTELE       YRPGQALMLW
Sbjct: 61  LFALDDSSSDF------FMSDKREILKNDSKVLLKGLSYTELEKWVQSRGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKI 183
           KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRK---------- 180

Query: 184 HLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIM 243
                      ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    M
Sbjct: 181 -----------ILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----M 240

Query: 244 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 303
           GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL
Sbjct: 241 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 300

Query: 304 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL
Sbjct: 301 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 360

Query: 364 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 423
           REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR
Sbjct: 361 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 420

Query: 424 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLAL 479
           REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLAL
Sbjct: 421 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLAL 449

[Close]

BLAST of MS000882.1 vs. ExPASy TrEMBL
Match: A0A6J1HD79 (uncharacterized protein LOC111462441 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462441 PE=4 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 4.6e-208
Identity = 391/479 (81.63%), Postives = 408/479 (85.18%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPK SMASSIL  A S F  LS+PS+RL  P HLISF+ SA R  +TAS ASPTP
Sbjct: 1   MRRLFFPKPSMASSIL--APSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPTP 60

Query: 64  LFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE----AR-YRPGQALMLW 123
           LFALDDSS DF       M +K+EIL  DSK+LLKGLSYTELE    AR YRPGQALMLW
Sbjct: 61  LFALDDSSPDFS------MPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKI 183
           KRLYGDN+WAH+GDELEGLNK FKKMLIE AEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK---------- 180

Query: 184 HLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIM 243
                      ILF+LEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    M
Sbjct: 181 -----------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----M 240

Query: 244 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 303
           GLKRHLTAAEIVEQAVFARRLLT +VG ITNVVFMGMGEPLHNIDNV KAANIMV EQGL
Sbjct: 241 GLKRHLTAAEIVEQAVFARRLLTCDVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGL 300

Query: 304 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFL +C+CALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL
Sbjct: 301 HFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 360

Query: 364 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 423
           REEL  KHNYKV FEYVMLAGVND +EDAKR+VDLVQGIPCKINLISFNPH GSQFRPT 
Sbjct: 361 REELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTC 420

Query: 424 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLA 478
           +EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGTIQAPLLRVP+QFQMAMKLA
Sbjct: 421 KEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLA 446

[Close]

BLAST of MS000882.1 vs. ExPASy TrEMBL
Match: A0A6J1K720 (uncharacterized protein LOC111491756 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491756 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.0e-207
Identity = 391/479 (81.63%), Postives = 407/479 (84.97%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLISFVFSASRPISTASCASPTP 63
           MRRLFFPK SMASSIL  A S F  LS+PS+RL  P HLISF+ SA R  +TAS ASP P
Sbjct: 1   MRRLFFPKPSMASSIL--APSHFYSLSIPSARLCPPLHLISFLSSAGRRFTTASSASPKP 60

Query: 64  LFALDDSSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE----AR-YRPGQALMLW 123
           LFALDDSS DF       M +K+EIL  DSK+LLKGLSYTELE    AR YRPGQALMLW
Sbjct: 61  LFALDDSSPDFS------MPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLW 120

Query: 124 KRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKI 183
           KRLYGDN+WAH+GDELEGLNK FKKMLIE AEFRALSLREILPSSDGTRK          
Sbjct: 121 KRLYGDNVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK---------- 180

Query: 184 HLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIM 243
                      ILF+LEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    M
Sbjct: 181 -----------ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----M 240

Query: 244 GLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGL 303
           GLKRHLTAAEIVEQAVFARRLLT EVG ITNVVFMGMGEPLHNIDNV KAANIMV EQGL
Sbjct: 241 GLKRHLTAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGL 300

Query: 304 HFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 363
           HFSPRKVTVSTSGLVPQLKRFL +C+CALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL
Sbjct: 301 HFSPRKVTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTL 360

Query: 364 REELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTR 423
           REEL  KHNYKV FEYVMLAGVND +EDAKR+VDLVQGIPCKINLISFNPH GSQFRPT 
Sbjct: 361 REELRCKHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTC 420

Query: 424 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLA 478
           +EKMIEFRNVLA AGLTV LRLSRGDDQMAACGQLGKPGTIQAPLLRVP+QFQMAMKLA
Sbjct: 421 KEKMIEFRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLA 446

[Close]

BLAST of MS000882.1 vs. ExPASy TrEMBL
Match: A0A6J1K524 (uncharacterized protein LOC111491756 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491756 PE=4 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 5.0e-186
Identity = 360/473 (76.11%), Postives = 382/473 (80.76%), Query Frame = 0


Query: 11  KASMASSILAVARSPFRPLSVPS-SRLSLPFHLISFVFSASRPISTASCASPTPLFALDD 70
           K +M++ + +   S     SVP  +   LP  L S V S     S  + A  T L +L +
Sbjct: 36  KPTMSAPLPSAKPSASSRFSVPKLTNFRLPVEL-SSVQSLMPLHSATASALFTSLLSLHN 95

Query: 71  SSSDFCHCHLVVMSDKREILKNDSKVLLKGLSYTELE----AR-YRPGQALMLWKRLYGD 130
           +S     C    M +K+EIL  DSK+LLKGLSYTELE    AR YRPGQALMLWKRLYGD
Sbjct: 96  NS---WGCLSEAMPEKKEILHTDSKMLLKGLSYTELEKWVKARGYRPGQALMLWKRLYGD 155

Query: 131 NIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFLWICALKIHLIFKA 190
           N+WAH+GDELEGLNK FKKMLIE AEFRALSLREILPSSDGTRK                
Sbjct: 156 NVWAHSGDELEGLNKDFKKMLIEKAEFRALSLREILPSSDGTRK---------------- 215

Query: 191 IRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHL 250
                ILF+LEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR    MGLKRHL
Sbjct: 216 -----ILFNLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYTGR----MGLKRHL 275

Query: 251 TAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRK 310
           TAAEIVEQAVFARRLLT EVG ITNVVFMGMGEPLHNIDNV KAANIMV EQGLHFSPRK
Sbjct: 276 TAAEIVEQAVFARRLLTCEVGLITNVVFMGMGEPLHNIDNVIKAANIMVDEQGLHFSPRK 335

Query: 311 VTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELCS 370
           VTVSTSGLVPQLKRFL +C+CALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREEL  
Sbjct: 336 VTVSTSGLVPQLKRFLHDCNCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELRC 395

Query: 371 KHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSGSQFRPTRREKMIE 430
           KHNYKV FEYVMLAGVND +EDAKR+VDLVQGIPCKINLISFNPH GSQFRPT +EKMIE
Sbjct: 396 KHNYKVLFEYVMLAGVNDSIEDAKRIVDLVQGIPCKINLISFNPHCGSQFRPTCKEKMIE 455

Query: 431 FRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQMAMKLA 478
           FRNVLA AGLTV LRLSRGDDQMAACGQLGKPGTIQAPLLRVP+QFQMAMKLA
Sbjct: 456 FRNVLAAAGLTVLLRLSRGDDQMAACGQLGKPGTIQAPLLRVPDQFQMAMKLA 479

[Close]

BLAST of MS000882.1 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 556.6 bits (1433), Expect = 1.9e-158
Identity = 307/487 (63.04%), Postives = 357/487 (73.31%), Query Frame = 0


Query: 4   MRRLFFPKASMASSILAVARSPFRPLSVPSSRLSLPFHLI------SFVFSASRPISTAS 63
           MRRL     ++ +S   V  S     +     L+LP  L+      S +F  SR +S  S
Sbjct: 5   MRRLGI--ITLTNSAAVVTPSLLSSTAAFPGTLTLPSPLLSDRLTNSILFHESRSLS-FS 64

Query: 64  CASPTPLFALDDSSSDFCHCHLVVMSDKREILKNDS-KVLLKGLSYTELEA-----RYRP 123
           C S +       SS+ +        S K  + ++D+ KV+LKG++Y  L+       +RP
Sbjct: 65  CFSSS-----SSSSAAYLPSLEEYPSAKGSVKRDDNQKVVLKGMTYASLQEWVQSHGFRP 124

Query: 124 GQALMLWKRLYGDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSDGTRKVFL 183
           GQALMLWKRLY DNIWA+  DELEGLNK  K+M+ E+AEF ALS ++I  +SDGTRK   
Sbjct: 125 GQALMLWKRLYKDNIWANNVDELEGLNKDLKRMISEHAEFGALSFKDIRSASDGTRK--- 184

Query: 184 WICALKIHLIFKAIRTFQILFDLEDGLIIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYT 243
                             ILF L+DGL+IETVVIPCDRGRTTVCVSSQVGCAMNCQFCYT
Sbjct: 185 ------------------ILFTLDDGLVIETVVIPCDRGRTTVCVSSQVGCAMNCQFCYT 244

Query: 244 GRQASIMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANI 303
           GR    MGLKR+LT AEIVEQAV+ARRLL+ EVGSITNVVFMGMGEP HNIDNV KAANI
Sbjct: 245 GR----MGLKRNLTTAEIVEQAVYARRLLSHEVGSITNVVFMGMGEPFHNIDNVIKAANI 304

Query: 304 MVHEQGLHFSPRKVTVSTSGLVPQLKRFLRECDCALAVSLNATTDEVRNWIMPINRKYKL 363
           MV E GLHFSPRKVTVSTSGLVPQLKRFLRE +CALAVSLNATTDEVRNWIMPINRKYKL
Sbjct: 305 MVDENGLHFSPRKVTVSTSGLVPQLKRFLRESNCALAVSLNATTDEVRNWIMPINRKYKL 364

Query: 364 GLLLQTLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQGIPCKINLISFNPHSG 423
            LLL+TLRE L S+H YKV FEYVMLAGVND ++DA+RLV+LVQGIPCKINLI FNPHSG
Sbjct: 365 SLLLETLREGLSSRHKYKVLFEYVMLAGVNDSMDDARRLVELVQGIPCKINLIQFNPHSG 424

Query: 424 SQFRPTRREKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQLGKPGTIQAPLLRVPEQFQ 479
           SQF  T  +KMI+FRNVLAE G TV +R SRG+DQMAACGQLG  G +QAP++RVPEQF+
Sbjct: 425 SQFIQTEEDKMIKFRNVLAEGGCTVLMRFSRGNDQMAACGQLGMIGAVQAPVMRVPEQFR 458

[Close]

BLAST of MS000882.1 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 2.9e-45
Identity = 130/380 (34.21%), Postives = 186/380 (48.95%), Query Frame = 0


Query: 107 ARYRPGQALMLWKRLY--GDNIWAHTGDELEGLNKYFKKMLIENAEFRALSLREILPSSD 166
           A   P  A+ +WK +    D +W    DE+  L      +L    +    SL  +  SSD
Sbjct: 19  AGINPKFAIQIWKYVIQNPDCVW----DEIPSLPSAAYSLLHSKFKTLTSSLHSLFHSSD 78

Query: 167 GTRKVFLWICALKIHLIFKAIRTFQILFDLEDGLIIETVVIPCDRG-------------R 226
           GT                    T ++L  L++G  +E VV+  D               R
Sbjct: 79  GT--------------------TSKLLIKLQNGAFVEAVVMRYDTRLGMLGGKPRPGGIR 138

Query: 227 TTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHLTAAEIVEQAVFARRLLTSEVGSITNVV 286
           +T+C+SSQVGC M C FC TG     MG K +LT+ EIVEQ V A     S +  I N+V
Sbjct: 139 STLCISSQVGCKMGCTFCATG----TMGFKSNLTSGEIVEQLVHA-----SRIADIRNIV 198

Query: 287 FMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFLREC-DCALAVS 346
           FMGMGEPL+N + V +A  +M++ Q    SP+++T+ST G+V  + +   +    +LAVS
Sbjct: 199 FMGMGEPLNNYNAVVEAVRVMLN-QPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVS 258

Query: 347 LNATTDEVRNWIMPINRKYKLGLLLQTLREELCSKHNYKVFFEYVMLAGVNDGLEDAKRL 406
           L+A   E+R  IMP  R + L  L+  L +        K+F EY+ML GVND  + A  L
Sbjct: 259 LHAPVQEIRCQIMPAARAFPLQKLMDAL-QTFQKNSQQKIFIEYIMLDGVNDQEQHAHLL 318

Query: 407 VDLVQGIPCKINLISFNP-HSGSQFRPTRREKMIEFRNVLAEA-GLTVFLRLSRGDDQMA 461
            +L++     INLI FNP  S SQF  +  + +  F+ +L E   +   +R   G D   
Sbjct: 319 GELLKTFQVVINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISG 363

[Close]

BLAST of MS000882.1 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 4.3e-41
Identity = 104/275 (37.82%), Postives = 154/275 (56.00%), Query Frame = 0


Query: 187 TFQILFDLEDGLIIETVVIPC---DRG--RTTVCVSSQVGCAMNCQFCYTGRQASIMGLK 246
           T ++L  LED L+IETV IP    ++G  R T CVSSQVGC + C FC TG+      L+
Sbjct: 137 TIKLLLKLEDNLLIETVGIPVQDDEKGITRLTACVSSQVGCPLRCSFCATGKGGFSRNLQ 196

Query: 247 RHLTAAEIVEQAVFARRLLTSEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFS 306
           RH    EI+EQ +    +    V   TNVVFMGMGEP+ N+ +V  A   +   + +   
Sbjct: 197 RH----EIIEQVLAIEDVFKHRV---TNVVFMGMGEPMLNLKSVLDAHRCL--NKDIEIG 256

Query: 307 PRKVTVSTSGLVPQLKRFL-RECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLRE 366
            R +T+ST G+   +K+    +    LAVSL+A    +R  I+P  + Y L  +++  R+
Sbjct: 257 QRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAIMKDCRD 316

Query: 367 ELCSKHNYKVFFEYVMLAGVNDGLEDAKRLVDLVQ--GIPCKINLISFNPHSGSQFRPTR 426
               + N +V FEY +LAGVND +E A  L +L++  G    +NLI +NP  GS+++   
Sbjct: 317 YF-QETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSEYQRPY 376

Query: 427 REKMIEFRNVLAEAGLTVFLRLSRGDDQMAACGQL 454
           ++ ++ F   L    +T  +R +RG D  AACGQL
Sbjct: 377 KKAVLAFAAALESRKITASVRQTRGLDASAACGQL 401

[Close]

BLAST of MS000882.1 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 7.4e-41
Identity = 134/435 (30.80%), Postives = 213/435 (48.97%), Query Frame = 0


Query: 30  SVPSSRLSLPFHLISFVFSASRPISTASCASPTPLFALDDSSSDFCHCHLVVMSDK--RE 89
           SVP SR+ +  H ++  FSA+   +T    SP P+ A    +       L+ MS+   +E
Sbjct: 18  SVPISRMVVRRHSLATTFSAA---ATTVVPSPKPVSAKPARTPHVDSHVLIGMSEPELQE 77

Query: 90  ILKNDSKVLLKGLSYTELEARYRPGQALMLWKRLYGDNIWAHTGDELEGLNKYFKKMLIE 149
           +  N   +  +G    +L          +++KR        +  ++   L   F+K L++
Sbjct: 78  LAINLVLIFQEGYRGKQLHH--------LIYKRK------VNKVEDFSNLPLTFRKGLVD 137

Query: 150 NA-EFRALSLREILPSSDGTRKVFLWICALKIHLIFKAIRTFQILFDLEDGLIIETVVIP 209
              +     + + + ++DGT K                     +L  LED L+IETV IP
Sbjct: 138 GGFKVGRSPIYQTVTATDGTIK---------------------LLLKLEDNLLIETVGIP 197

Query: 210 C---DRG--RTTVCVSSQVGCAMNCQFCYTGRQASIMGLKRHLTAAEIVEQAVFARRLLT 269
               ++G  R T CVSSQVGC + C FC TG+      L+RH    EI+EQ +    +  
Sbjct: 198 VQDDEKGITRLTACVSSQVGCPLRCSFCATGKGGFSRNLQRH----EIIEQVLAIEDVFK 257

Query: 270 SEVGSITNVVFMGMGEPLHNIDNVTKAANIMVHEQGLHFSPRKVTVSTSGLVPQLKRFL- 329
             V   TNVVFMGMGEP+ N+ +V  A   +   + +    R +T+ST G+   +K+   
Sbjct: 258 HRV---TNVVFMGMGEPMLNLKSVLDAHRCL--NKDIEIGQRMITISTVGVPNTIKKLAS 317

Query: 330 RECDCALAVSLNATTDEVRNWIMPINRKYKLGLLLQTLREELCSKHNYKVFFEYVMLAGV 389
            +    LAVSL+A    +R  I+P  + Y L  +++  R+    + N +V FEY +LAGV
Sbjct: 318 HKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAIMKDCRDYF-QETNRRVSFEYALLAGV 377

Query: 390 NDGLEDAKRLVDLVQ--GIPCKINLISFNPHSGSQFRPTRREKMIEFRNVLAEAGLTVFL 449
           ND +E A  L +L++  G    +NLI +NP  GS+++   ++ ++ F   L    +T  +
Sbjct: 378 NDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSEYQRPYKKAVLAFAAALESRKITASV 404

Query: 450 RLSRGDDQMAACGQL 454
           R +RG D  AACGQL
Sbjct: 438 RQTRGLDASAACGQL 404

The following BLAST results are available for this feature:

BLAST of MS000882.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159137.1	6.5e-241	91.49	uncharacterized protein LOC111025565 isoform X1 [Momordica charantia]	[more]
XP_022159138.1	7.2e-240	91.67	uncharacterized protein LOC111025565 isoform X2 [Momordica charantia]	[more]
XP_023546952.1	4.3e-208	81.84	uncharacterized protein LOC111805899 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022961770.1	9.5e-208	81.63	uncharacterized protein LOC111462441 isoform X1 [Cucurbita moschata]	[more]
XP_022996535.1	2.1e-207	81.63	uncharacterized protein LOC111491756 isoform X1 [Cucurbita maxima]	[more]

BLAST of MS000882.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q74E53	9.6e-70	45.14	Dual-specificity RNA methyltransferase RlmN OS=Geobacter sulfurreducens (strain ...	[more]
Q3A2Z4	1.1e-68	41.15	Dual-specificity RNA methyltransferase RlmN OS=Pelobacter carbinolicus (strain D...	[more]
Q39S71	3.1e-68	45.14	Dual-specificity RNA methyltransferase RlmN OS=Geobacter metallireducens (strain...	[more]
A0LQM1	4.2e-65	42.15	Dual-specificity RNA methyltransferase RlmN OS=Syntrophobacter fumaroxidans (str...	[more]
A5GEC2	7.1e-65	43.17	Dual-specificity RNA methyltransferase RlmN OS=Geobacter uraniireducens (strain ...	[more]

BLAST of MS000882.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYZ7	3.2e-241	91.49	uncharacterized protein LOC111025565 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DXZ7	3.5e-240	91.67	uncharacterized protein LOC111025565 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1HD79	4.6e-208	81.63	uncharacterized protein LOC111462441 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1K720	1.0e-207	81.63	uncharacterized protein LOC111491756 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1K524	5.0e-186	76.11	uncharacterized protein LOC111491756 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of MS000882.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60230.1	1.9e-158	63.04	Radical SAM superfamily protein	[more]
AT3G19630.1	2.9e-45	34.21	Radical SAM superfamily protein	[more]
AT2G39670.1	4.3e-41	37.82	Radical SAM superfamily protein	[more]
AT2G39670.2	7.4e-41	30.80	Radical SAM superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF13394	Fer4_14	coord: 221..329 e-value: 1.3E-6 score: 28.4
None	No IPR available	SFLD	SFLDG01062	methyltransferase_(Class_A)	coord: 110..455 e-value: 0.0 score: 344.8
None	No IPR available	PANTHER	PTHR30544:SF5	RADICAL SAM SUPERFAMILY PROTEIN	coord: 70..461
None	No IPR available	CDD	cd01335	Radical_SAM	coord: 221..428 e-value: 1.57377E-5 score: 43.8614
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 210..434
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 217..386 e-value: 7.8E-17 score: 62.2
IPR007197	Radical SAM	PROSITE	PS51918	RADICAL_SAM	coord: 208..445 score: 30.406742
IPR027492	Dual-specificity RNA methyltransferase RlmN	TIGRFAM	TIGR00048	TIGR00048	coord: 187..454 e-value: 4.7E-89 score: 296.7
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 181..459 e-value: 4.4E-105 score: 352.9
IPR004383	Ribosomal RNA large subunit methyltransferase RlmN/Cfr	SFLD	SFLDF00275	adenosine_C2_methyltransferas	coord: 110..455 e-value: 0.0 score: 344.8
IPR040072	Methyltransferase (Class A)	PANTHER	PTHR30544	23S RRNA METHYLTRANSFERASE	coord: 70..461

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS000882	MS000882	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS000882.1-cds	MS000882.1-cds-scaffold36:50114..50399	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:50527..50663	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:50752..50943	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:51536..51643	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:51718..51885	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:51888..51922	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:52029..52130	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:52291..52374	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:52520..52614	CDS
MS000882.1-cds	MS000882.1-cds-scaffold36:52774..53000	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS000882.1	MS000882.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
biological_process	GO:0006364	rRNA processing
biological_process	GO:0030488	tRNA methylation
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0008173	RNA methyltransferase activity