MS000464.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS000464.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Lipoyl(octanoyl) transferase
Location	scaffold44: 1891427 .. 1891516 (-)
Sequence length	90
RNA-Seq Expression	MS000464.1
Synteny	MS000464.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAGTTTGGGTTGGAGCCATTGGTGTTAGAATTTCAGGTGGGATCACTTCTCATGGTCTGGCTTTCAATGTGGATCCTCATTTGAGGTAT

mRNA sequence

GGAGTTTGGGTTGGAGCCATTGGTGTTAGAATTTCAGGTGGGATCACTTCTCATGGTCTGGCTTTCAATGTGGATCCTCATTTGAGGTAT

Coding sequence (CDS)

GGAGTTTGGGTTGGAGCCATTGGTGTTAGAATTTCAGGTGGGATCACTTCTCATGGTCTGGCTTTCAATGTGGATCCTCATTTGAGGTAT

Protein sequence

GVWVGAIGVRISGGITSHGLAFNVDPHLRY

Homology

[Close]

BLAST of MS000464.1 vs. NCBI nr
Match: XP_038879976.1 (octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octanoyltransferase LIP2, mitochondrial [Benincasa hispida])

HSP 1 Score: 55.1 bits (131), Expect = 1.2e-04
Identity = 28/35 (80.00%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRIS GITSHGLAFNVDP LRY
Sbjct: 137 GVWVGDRKIGAIGVRISNGITSHGLAFNVDPDLRY 171

[Close]

BLAST of MS000464.1 vs. NCBI nr
Match: XP_024983286.1 (octanoyltransferase-like [Cynara cardunculus var. scolymus] >XP_024983287.1 octanoyltransferase-like [Cynara cardunculus var. scolymus] >XP_024983288.1 octanoyltransferase-like [Cynara cardunculus var. scolymus] >XP_024983289.1 octanoyltransferase-like [Cynara cardunculus var. scolymus])

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-04
Identity = 27/35 (77.14%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRIS GITSHGLAFN+DP LRY
Sbjct: 137 GVWVGERKIGAIGVRISSGITSHGLAFNIDPDLRY 171

[Close]

BLAST of MS000464.1 vs. NCBI nr
Match: KVI00265.1 (Biotin/lipoate A/B protein ligase [Cynara cardunculus var. scolymus])

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-04
Identity = 27/35 (77.14%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRIS GITSHGLAFN+DP LRY
Sbjct: 730 GVWVGERKIGAIGVRISSGITSHGLAFNIDPDLRY 764

[Close]

BLAST of MS000464.1 vs. NCBI nr
Match: XP_022641414.1 (octanoyltransferase isoform X3 [Vigna radiata var. radiata])

HSP 1 Score: 53.9 bits (128), Expect = 2.7e-04
Identity = 26/35 (74.29%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRISGGITSHG+AFN+DP L Y
Sbjct: 137 GVWVGERKIGAIGVRISGGITSHGMAFNIDPDLSY 171

[Close]

BLAST of MS000464.1 vs. NCBI nr
Match: XP_022154074.1 (octanoyltransferase [Momordica charantia])

HSP 1 Score: 53.9 bits (128), Expect = 2.7e-04
Identity = 27/35 (77.14%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRISGGITSHGLAFN+DP L Y
Sbjct: 137 GVWVGDRKIGAIGVRISGGITSHGLAFNLDPDLTY 171

[Close]

BLAST of MS000464.1 vs. ExPASy Swiss-Prot
Match: Q9SXP7 (Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.0e-06
Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRIS GITSHGLA N+DP ++Y
Sbjct: 137 GVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKY 171

[Close]

BLAST of MS000464.1 vs. ExPASy TrEMBL
Match: A0A103Y0H2 (Selenoprotein O OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_021512 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 7.8e-05
Identity = 27/35 (77.14%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRIS GITSHGLAFN+DP LRY
Sbjct: 730 GVWVGERKIGAIGVRISSGITSHGLAFNIDPDLRY 764

[Close]

BLAST of MS000464.1 vs. ExPASy TrEMBL
Match: A0A1S3UY81 (Lipoyl(octanoyl) transferase OS=Vigna radiata var. radiata OX=3916 GN=LOC106769796 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-04
Identity = 26/35 (74.29%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRISGGITSHG+AFN+DP L Y
Sbjct: 137 GVWVGERKIGAIGVRISGGITSHGMAFNIDPDLSY 171

[Close]

BLAST of MS000464.1 vs. ExPASy TrEMBL
Match: A0A3Q0FFK4 (Lipoyl(octanoyl) transferase OS=Vigna radiata var. radiata OX=3916 GN=LOC106769796 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-04
Identity = 26/35 (74.29%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRISGGITSHG+AFN+DP L Y
Sbjct: 137 GVWVGERKIGAIGVRISGGITSHGMAFNIDPDLSY 171

[Close]

BLAST of MS000464.1 vs. ExPASy TrEMBL
Match: A0A3Q0FGE6 (Lipoyl(octanoyl) transferase OS=Vigna radiata var. radiata OX=3916 GN=LOC106769796 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-04
Identity = 26/35 (74.29%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRISGGITSHG+AFN+DP L Y
Sbjct: 162 GVWVGERKIGAIGVRISGGITSHGMAFNIDPDLSY 196

[Close]

BLAST of MS000464.1 vs. ExPASy TrEMBL
Match: A0A0L9U0B9 (Lipoyl(octanoyl) transferase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan02g235600 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-04
Identity = 26/35 (74.29%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRISGGITSHG+AFN+DP L Y
Sbjct: 137 GVWVGERKIGAIGVRISGGITSHGMAFNIDPDLSY 171

[Close]

BLAST of MS000464.1 vs. TAIR 10
Match: AT1G04640.1 (lipoyltransferase 2 )

HSP 1 Score: 50.4 bits (119), Expect = 2.8e-07
Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRIS GITSHGLA N+DP ++Y
Sbjct: 137 GVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKY 171

[Close]

BLAST of MS000464.1 vs. TAIR 10
Match: AT1G04640.2 (lipoyltransferase 2 )

HSP 1 Score: 50.4 bits (119), Expect = 2.8e-07
Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 0


Query: 1   GVWV-----GAIGVRISGGITSHGLAFNVDPHLRY 31
           GVWV     GAIGVRIS GITSHGLA N+DP ++Y
Sbjct: 137 GVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKY 171

The following BLAST results are available for this feature:

BLAST of MS000464.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879976.1	1.2e-04	80.00	octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octa...	[more]
XP_024983286.1	1.6e-04	77.14	octanoyltransferase-like [Cynara cardunculus var. scolymus] >XP_024983287.1 octa...	[more]
KVI00265.1	1.6e-04	77.14	Biotin/lipoate A/B protein ligase [Cynara cardunculus var. scolymus]	[more]
XP_022641414.1	2.7e-04	74.29	octanoyltransferase isoform X3 [Vigna radiata var. radiata]	[more]
XP_022154074.1	2.7e-04	77.14	octanoyltransferase [Momordica charantia]	[more]

BLAST of MS000464.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXP7	4.0e-06	68.57	Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 ...	[more]

BLAST of MS000464.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A103Y0H2	7.8e-05	77.14	Selenoprotein O OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_021512 PE=3...	[more]
A0A1S3UY81	1.3e-04	74.29	Lipoyl(octanoyl) transferase OS=Vigna radiata var. radiata OX=3916 GN=LOC1067697...	[more]
A0A3Q0FFK4	1.3e-04	74.29	Lipoyl(octanoyl) transferase OS=Vigna radiata var. radiata OX=3916 GN=LOC1067697...	[more]
A0A3Q0FGE6	1.3e-04	74.29	Lipoyl(octanoyl) transferase OS=Vigna radiata var. radiata OX=3916 GN=LOC1067697...	[more]
A0A0L9U0B9	1.3e-04	74.29	Lipoyl(octanoyl) transferase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan02g2356...	[more]

BLAST of MS000464.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G04640.1	2.8e-07	68.57	lipoyltransferase 2	[more]
AT1G04640.2	2.8e-07	68.57	lipoyltransferase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 1..30 e-value: 4.2E-7 score: 31.5
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 4..29
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PROSITE	PS51733	BPL_LPL_CATALYTIC	coord: 1..30 score: 12.15546

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS000464	MS000464	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS000464.1-cds	MS000464.1-cds-scaffold44:1891427..1891516	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS000464.1	MS000464.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process