MELO3C015328.1 (mRNA) Melon (DHL92) v4

BLAST of MELO3C015328.1 vs. NCBI nr
Match: XP_016901018.1 (PREDICTED: amidase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 844.0 bits (2179), Expect = 6.1e-241
Identity = 422/431 (97.91%), Postives = 422/431 (97.91%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV-- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV  
Sbjct: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 120

Query: 121 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180
                  GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK
Sbjct: 121 NLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180

Query: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240
           KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG
Sbjct: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240

Query: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300
           NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP
Sbjct: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300

Query: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 360
           GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP
Sbjct: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 360

Query: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420
           KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV
Sbjct: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420

Query: 421 VHSLYNTLEEE 423
           VHSLYNTLEEE
Sbjct: 421 VHSLYNTLEEE 431

BLAST of MELO3C015328.1 vs. NCBI nr
Match: XP_008450658.1 (PREDICTED: amidase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 819.3 bits (2115), Expect = 1.6e-233
Identity = 413/431 (95.82%), Postives = 413/431 (95.82%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV-- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV  
Sbjct: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 120

Query: 121 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180
                  GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK
Sbjct: 121 NLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180

Query: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240
           KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG
Sbjct: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240

Query: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300
           NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP
Sbjct: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300

Query: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 360
           GISERVSEAMRATDENIDLCHSIKIELRRALAALLE         DFGVLAIPTVPGPPP
Sbjct: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLE---------DFGVLAIPTVPGPPP 360

Query: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420
           KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV
Sbjct: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420

Query: 421 VHSLYNTLEEE 423
           VHSLYNTLEEE
Sbjct: 421 VHSLYNTLEEE 422

BLAST of MELO3C015328.1 vs. NCBI nr
Match: TYK10232.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 817.8 bits (2111), Expect = 4.7e-233
Identity = 413/435 (94.94%), Postives = 413/435 (94.94%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 46  MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 105

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA--- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA   
Sbjct: 106 APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 165

Query: 121 ----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 180
                     VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP
Sbjct: 166 NLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 225

Query: 181 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 240
           VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ
Sbjct: 226 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 285

Query: 241 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 300
           VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP
Sbjct: 286 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 345

Query: 301 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVP 360
           HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE         DFGVLAIPTVP
Sbjct: 346 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE---------DFGVLAIPTVP 405

Query: 361 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF 420
           GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF
Sbjct: 406 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF 465

Query: 421 LLNVVHSLYNTLEEE 423
           LLNVVHSLYNTLEEE
Sbjct: 466 LLNVVHSLYNTLEEE 471

BLAST of MELO3C015328.1 vs. NCBI nr
Match: KAA0050880.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 814.7 bits (2103), Expect = 4.0e-232
Identity = 412/435 (94.71%), Postives = 412/435 (94.71%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 46  MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 105

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA--- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA   
Sbjct: 106 APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 165

Query: 121 ----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 180
                     VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP
Sbjct: 166 NLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 225

Query: 181 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 240
           VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ
Sbjct: 226 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 285

Query: 241 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 300
           VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP
Sbjct: 286 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 345

Query: 301 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVP 360
           HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE         DFGVLAIPTVP
Sbjct: 346 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE---------DFGVLAIPTVP 405

Query: 361 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF 420
           GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPL LYNGLPVSISLVANHGSDGF
Sbjct: 406 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLRLYNGLPVSISLVANHGSDGF 465

Query: 421 LLNVVHSLYNTLEEE 423
           LLNVVHSLYNTLEEE
Sbjct: 466 LLNVVHSLYNTLEEE 471

BLAST of MELO3C015328.1 vs. NCBI nr
Match: XP_004135624.1 (amidase 1 [Cucumis sativus] >KGN66086.1 hypothetical protein Csa_006860 [Cucumis sativus])

HSP 1 Score: 792.3 bits (2045), Expect = 2.1e-225
Identity = 402/432 (93.06%), Postives = 406/432 (93.98%), Query Frame = 0

Query: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV- 120
           TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVF 180
                   GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQS DTVGWFARDPVV 
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNL 240
           KKVG LLLQQPEVEHQKP QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH IK+V+L
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHPIKEVSL 240

Query: 241 GNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPP 360
           PGISERVSEAMRATDENIDLCHSI+IELR+ALAALLE         DFGVLAIPTVPGPP
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIRIELRKALAALLE---------DFGVLAIPTVPGPP 360

Query: 361 PKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420
           PKLNTDVSEL DFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN
Sbjct: 361 PKLNTDVSELQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420

Query: 421 VVHSLYNTLEEE 423
           VVHSLYNTLEEE
Sbjct: 421 VVHSLYNTLEEE 423

BLAST of MELO3C015328.1 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.5e-144
Identity = 264/424 (62.26%), Postives = 317/424 (74.76%), Query Frame = 0

Query: 5   YGAFMEKFLLQPSSPSDQLP-LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPT 64
           +GAF+EK  + P+S S   P L GLTFA+KDIFD++G VTGFGNP+WLRTH  A  TAP 
Sbjct: 7   FGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPV 66

Query: 65  VSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV----- 124
           VS++L  GAT +G TIMDEMAYSINGEN HYGTP+NP A DRVPGGSSSGSAVAV     
Sbjct: 67  VSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLV 126

Query: 125 ----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFKKVG 184
               GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PMAQSFDTVGWFARD    K+VG
Sbjct: 127 DFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRVG 186

Query: 185 LLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH-LIKQVNLGNY 244
            +LLQQ  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FGG+ ++K+VNLG Y
Sbjct: 187 CVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGEY 246

Query: 245 VEEKVPSLKQFMIEGN--AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 304
           + + VPSLK FM   +     E  IPSL AL+ S++LLQR+EFKINH  W+ +  P  GP
Sbjct: 247 IGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFGP 306

Query: 305 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 364
           GISER+ EA+R +DE ID C S+K EL         +  LS +L + GVL IPTVPGPPP
Sbjct: 307 GISERIEEAIRTSDEKIDHCRSVKSEL---------ITALSTLLGEKGVLVIPTVPGPPP 366

Query: 365 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 416
            L  +V+ L  FR++AFSLLSIAGVSGFCQVSIPLGL+  LPVS+SLVA +GSDGFLL++
Sbjct: 367 HLQANVAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSL 421

BLAST of MELO3C015328.1 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 3.2e-131
Identity = 242/428 (56.54%), Postives = 302/428 (70.56%), Query Frame = 0

Query: 5   YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTV 64
           YGAFME+F+L P  PS QLPL GLTFA+KDIFD+ G VTGFGNP+W RTH PA  T+P V
Sbjct: 11  YGAFMERFVL-PPPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPVV 70

Query: 65  STILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV------ 124
              L  GAT +G TIMDEMAYSINGEN HYGTP NPCA  RVPGGSSSGSAVAV      
Sbjct: 71  LAALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLVD 130

Query: 125 ---GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFKKVGL 184
              GTDTGGSVRVPA+YCGI G RPSHG VS   V+PMAQ FDTVGWF+RD     +V  
Sbjct: 131 FSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTLSRVTK 190

Query: 185 LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVE 244
           +LL  P+   ++PTQV I  DCF++     +R  Q    SV K F   ++   NLG+++ 
Sbjct: 191 VLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDNRNLGDFIS 250

Query: 245 EKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISE 304
           + VPS+ +F+ + +     S+P+L+ ++  ++ LQR +FK NH EWV T  P+LGPG+ E
Sbjct: 251 DNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNLGPGLRE 310

Query: 305 RVSEAMRATD-ENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPPKLN 364
           R+ EA+ + D E+++   +I+ E + ALAALL+         D G+LAIPTVPGPPPK+ 
Sbjct: 311 RILEAIASGDNESLEDFQAIRAEFKSALAALLK---------DHGILAIPTVPGPPPKVG 370

Query: 365 TDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHS 423
            + + L +FRA+AFSLLSIAG+SGFCQVSIPLG+ NGLPVS+SLVA HG+D FLLNVV  
Sbjct: 371 MEAAPLENFRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEE 428

BLAST of MELO3C015328.1 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 5.1e-113
Identity = 211/456 (46.27%), Postives = 292/456 (64.04%), Query Frame = 0

Query: 5   YGAFMEKFLL----QPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 64
           +GAF+ K  L     P+ P    PL+ L FA+ DIFD++GHV+ FG+PEW RTH PA+ T
Sbjct: 37  FGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPASST 96

Query: 65  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV-- 124
           A  VS ++  GATCIG T++DE+AY I+GEN H+GTP NP   +RVPGGSSSG+AVAV  
Sbjct: 97  ASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAVAA 156

Query: 125 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 184
                  G DT G VRVPA +CGILGFRPSHGAVS  G++P++ S DTVGWFA+DP V +
Sbjct: 157 NFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGWFAKDPDVLR 216

Query: 185 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 244
           +VG +LLQ P V  + P Q++IA+DCF+  ++P +R +Q  + + +KLFG  ++K +N  
Sbjct: 217 RVGHILLQAPFVMQRNPRQIIIADDCFQHLNVPLDRTSQVVIKATEKLFGKQVLKHINFE 276

Query: 245 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 304
           +Y+  KV SLK   I+ + G   S  SL  LA  +Q LQR+EF+  H EW+    P L P
Sbjct: 277 DYISSKVSSLKACSIQKSNGVLKS-SSLKLLANVMQSLQRHEFEHTHSEWMSIVKPDLHP 336

Query: 305 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 364
            +S ++ E    ++  I+   S++ ELR A+ +LL+         D GVL IPTV  PPP
Sbjct: 337 AVSAQLHEKFEVSELEIENSKSVRSELRVAVNSLLK---------DEGVLVIPTVADPPP 396

Query: 365 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 424
           KL       HD++++A SLLSIA +SG CQV++PLG ++  PVS+SL+A HG D FLL+ 
Sbjct: 397 KLGGKEFLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDT 456

Query: 425 VHSLYNTLEEEQWINLSSLDDEIFVFHRPSLAVDNE 448
           + ++Y  L+E+  I   S   +  V    S  +  E
Sbjct: 457 LKTMYTVLQEQADIAAPSKSSKSVVSKEQSAEISKE 482

BLAST of MELO3C015328.1 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.9e-112
Identity = 202/430 (46.98%), Postives = 285/430 (66.28%), Query Frame = 0

Query: 5   YGAFMEKFLL----QPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 64
           +GAF++K +L    QP+ P    PLTGLTFAV D+FD+ G+VTGFG+P+W+RTH  A+ T
Sbjct: 34  FGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAASST 93

Query: 65  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV-- 124
           +P VST++ GGATC+G+T++DE A+SI+GEN HY +P NP A  R+PGG+ SG+AVAV  
Sbjct: 94  SPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAVAT 153

Query: 125 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 184
                  G DT G VRVPA YCG+LGF+ S+GA+S +G++P++ S D+VGWFARDP   +
Sbjct: 154 NAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNTLR 213

Query: 185 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 244
           +VG +LLQ P    + P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K  NL 
Sbjct: 214 RVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQNLE 273

Query: 245 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 304
            Y E KVPSLK+F       N   + +   LA  +QLLQR+EF  NH +W+ T  P + P
Sbjct: 274 TYFETKVPSLKEFARTKAIANT-KVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAIDP 333

Query: 305 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 364
            I  +V E    T+E  +  ++I+ E R A+ +LL+         D G+L IPT+P  PP
Sbjct: 334 VILSQVCENPELTNEETENLNAIRNETRVAIGSLLK---------DDGILVIPTLPAVPP 393

Query: 365 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 422
           KL +      D++ +A SLLSIA +SG CQV++PLG +   P+S+S +  HG D FLL+ 
Sbjct: 394 KLGSKEITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDT 453

BLAST of MELO3C015328.1 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.4e-99
Identity = 193/444 (43.47%), Postives = 282/444 (63.51%), Query Frame = 0

Query: 5   YGAFMEKFLL----QPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 64
           +GAF+++F L     P  P+ +  L+GLTF++ D FD+  ++TGFG P+W +TH  A  T
Sbjct: 47  FGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKT 106

Query: 65  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGT 124
           A  V+T+L+ GATC+G+TIMDE+ + I GEN HYGTP NP   D VPGG SSGSAV+VG 
Sbjct: 107 AVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGA 166

Query: 125 ---------DTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 184
                    DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA DP V  
Sbjct: 167 ELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSVLC 226

Query: 185 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 244
           +VG  LL    V H++   ++ A+D F+LS IP ++  Q    +++ L G    K VN+G
Sbjct: 227 QVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVNVG 286

Query: 245 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 304
            YV   VPSL +F  E +  +++S  +L AL+  +  +QR+EFK NHEEW +T    LGP
Sbjct: 287 QYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFLGP 346

Query: 305 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 364
             S  V  A+++ +E+I   + +K E+R  + +LL+         + G+L IPTV  PPP
Sbjct: 347 RFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLK---------EDGILVIPTVADPPP 406

Query: 365 KLNTDVSE-LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 424
           +LNT  ++ L++F  + ++L  IA +SG CQV+IPLG +   P+S+SL+  +G D FLL+
Sbjct: 407 RLNTKRNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLD 466

Query: 425 VVHSLYNTLEEEQWI--NLSSLDD 433
               +Y +L+++  +  NL+ + D
Sbjct: 467 TTLDVYASLQDQAKLASNLAPVSD 480

BLAST of MELO3C015328.1 vs. ExPASy TrEMBL
Match: A0A1S4DYH0 (amidase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.0e-241
Identity = 422/431 (97.91%), Postives = 422/431 (97.91%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV-- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV  
Sbjct: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 120

Query: 121 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180
                  GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK
Sbjct: 121 NLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180

Query: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240
           KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG
Sbjct: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240

Query: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300
           NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP
Sbjct: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300

Query: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 360
           GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP
Sbjct: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 360

Query: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420
           KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV
Sbjct: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420

Query: 421 VHSLYNTLEEE 423
           VHSLYNTLEEE
Sbjct: 421 VHSLYNTLEEE 431

BLAST of MELO3C015328.1 vs. ExPASy TrEMBL
Match: A0A1S3BPN7 (amidase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 7.8e-234
Identity = 413/431 (95.82%), Postives = 413/431 (95.82%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV-- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV  
Sbjct: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 120

Query: 121 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180
                  GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK
Sbjct: 121 NLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 180

Query: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240
           KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG
Sbjct: 181 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 240

Query: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300
           NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP
Sbjct: 241 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 300

Query: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 360
           GISERVSEAMRATDENIDLCHSIKIELRRALAALLE         DFGVLAIPTVPGPPP
Sbjct: 301 GISERVSEAMRATDENIDLCHSIKIELRRALAALLE---------DFGVLAIPTVPGPPP 360

Query: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420
           KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV
Sbjct: 361 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 420

Query: 421 VHSLYNTLEEE 423
           VHSLYNTLEEE
Sbjct: 421 VHSLYNTLEEE 422

BLAST of MELO3C015328.1 vs. ExPASy TrEMBL
Match: A0A5D3CG55 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003790 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 413/435 (94.94%), Postives = 413/435 (94.94%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 46  MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 105

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA--- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA   
Sbjct: 106 APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 165

Query: 121 ----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 180
                     VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP
Sbjct: 166 NLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 225

Query: 181 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 240
           VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ
Sbjct: 226 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 285

Query: 241 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 300
           VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP
Sbjct: 286 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 345

Query: 301 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVP 360
           HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE         DFGVLAIPTVP
Sbjct: 346 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE---------DFGVLAIPTVP 405

Query: 361 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF 420
           GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF
Sbjct: 406 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF 465

Query: 421 LLNVVHSLYNTLEEE 423
           LLNVVHSLYNTLEEE
Sbjct: 466 LLNVVHSLYNTLEEE 471

BLAST of MELO3C015328.1 vs. ExPASy TrEMBL
Match: A0A5A7U6R8 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00050 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.9e-232
Identity = 412/435 (94.71%), Postives = 412/435 (94.71%), Query Frame = 0

Query: 1   MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 60
           MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT
Sbjct: 46  MAVQYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 105

Query: 61  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA--- 120
           APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVA   
Sbjct: 106 APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGA 165

Query: 121 ----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 180
                     VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP
Sbjct: 166 NLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDP 225

Query: 181 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 240
           VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ
Sbjct: 226 VVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQ 285

Query: 241 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 300
           VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP
Sbjct: 286 VNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNP 345

Query: 301 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVP 360
           HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE         DFGVLAIPTVP
Sbjct: 346 HLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLE---------DFGVLAIPTVP 405

Query: 361 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGF 420
           GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPL LYNGLPVSISLVANHGSDGF
Sbjct: 406 GPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLRLYNGLPVSISLVANHGSDGF 465

Query: 421 LLNVVHSLYNTLEEE 423
           LLNVVHSLYNTLEEE
Sbjct: 466 LLNVVHSLYNTLEEE 471

BLAST of MELO3C015328.1 vs. ExPASy TrEMBL
Match: A0A0A0M1K1 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G570110 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.0e-225
Identity = 402/432 (93.06%), Postives = 406/432 (93.98%), Query Frame = 0

Query: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV- 120
           TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVF 180
                   GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQS DTVGWFARDPVV 
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNL 240
           KKVG LLLQQPEVEHQKP QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH IK+V+L
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHPIKEVSL 240

Query: 241 GNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPP 360
           PGISERVSEAMRATDENIDLCHSI+IELR+ALAALLE         DFGVLAIPTVPGPP
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIRIELRKALAALLE---------DFGVLAIPTVPGPP 360

Query: 361 PKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420
           PKLNTDVSEL DFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN
Sbjct: 361 PKLNTDVSELQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420

Query: 421 VVHSLYNTLEEE 423
           VVHSLYNTLEEE
Sbjct: 421 VVHSLYNTLEEE 423

BLAST of MELO3C015328.1 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 514.2 bits (1323), Expect = 1.0e-145
Identity = 264/424 (62.26%), Postives = 317/424 (74.76%), Query Frame = 0

Query: 5   YGAFMEKFLLQPSSPSDQLP-LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPT 64
           +GAF+EK  + P+S S   P L GLTFA+KDIFD++G VTGFGNP+WLRTH  A  TAP 
Sbjct: 7   FGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPV 66

Query: 65  VSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV----- 124
           VS++L  GAT +G TIMDEMAYSINGEN HYGTP+NP A DRVPGGSSSGSAVAV     
Sbjct: 67  VSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLV 126

Query: 125 ----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFKKVG 184
               GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PMAQSFDTVGWFARD    K+VG
Sbjct: 127 DFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRVG 186

Query: 185 LLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH-LIKQVNLGNY 244
            +LLQQ  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FGG+ ++K+VNLG Y
Sbjct: 187 CVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGEY 246

Query: 245 VEEKVPSLKQFMIEGN--AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 304
           + + VPSLK FM   +     E  IPSL AL+ S++LLQR+EFKINH  W+ +  P  GP
Sbjct: 247 IGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFGP 306

Query: 305 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 364
           GISER+ EA+R +DE ID C S+K EL         +  LS +L + GVL IPTVPGPPP
Sbjct: 307 GISERIEEAIRTSDEKIDHCRSVKSEL---------ITALSTLLGEKGVLVIPTVPGPPP 366

Query: 365 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 416
            L  +V+ L  FR++AFSLLSIAGVSGFCQVSIPLGL+  LPVS+SLVA +GSDGFLL++
Sbjct: 367 HLQANVAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSL 421

BLAST of MELO3C015328.1 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 407.5 bits (1046), Expect = 1.4e-113
Identity = 202/430 (46.98%), Postives = 285/430 (66.28%), Query Frame = 0

Query: 5   YGAFMEKFLL----QPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 64
           +GAF++K +L    QP+ P    PLTGLTFAV D+FD+ G+VTGFG+P+W+RTH  A+ T
Sbjct: 34  FGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAASST 93

Query: 65  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV-- 124
           +P VST++ GGATC+G+T++DE A+SI+GEN HY +P NP A  R+PGG+ SG+AVAV  
Sbjct: 94  SPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAVAT 153

Query: 125 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 184
                  G DT G VRVPA YCG+LGF+ S+GA+S +G++P++ S D+VGWFARDP   +
Sbjct: 154 NAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNTLR 213

Query: 185 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 244
           +VG +LLQ P    + P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K  NL 
Sbjct: 214 RVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQNLE 273

Query: 245 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 304
            Y E KVPSLK+F       N   + +   LA  +QLLQR+EF  NH +W+ T  P + P
Sbjct: 274 TYFETKVPSLKEFARTKAIANT-KVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAIDP 333

Query: 305 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 364
            I  +V E    T+E  +  ++I+ E R A+ +LL+         D G+L IPT+P  PP
Sbjct: 334 VILSQVCENPELTNEETENLNAIRNETRVAIGSLLK---------DDGILVIPTLPAVPP 393

Query: 365 KLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNV 422
           KL +      D++ +A SLLSIA +SG CQV++PLG +   P+S+S +  HG D FLL+ 
Sbjct: 394 KLGSKEITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDT 453

BLAST of MELO3C015328.1 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-100
Identity = 193/444 (43.47%), Postives = 282/444 (63.51%), Query Frame = 0

Query: 5   YGAFMEKFLL----QPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHT 64
           +GAF+++F L     P  P+ +  L+GLTF++ D FD+  ++TGFG P+W +TH  A  T
Sbjct: 47  FGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKT 106

Query: 65  APTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGT 124
           A  V+T+L+ GATC+G+TIMDE+ + I GEN HYGTP NP   D VPGG SSGSAV+VG 
Sbjct: 107 AVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGA 166

Query: 125 ---------DTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVFK 184
                    DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA DP V  
Sbjct: 167 ELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSVLC 226

Query: 185 KVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLG 244
           +VG  LL    V H++   ++ A+D F+LS IP ++  Q    +++ L G    K VN+G
Sbjct: 227 QVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVNVG 286

Query: 245 NYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 304
            YV   VPSL +F  E +  +++S  +L AL+  +  +QR+EFK NHEEW +T    LGP
Sbjct: 287 QYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFLGP 346

Query: 305 GISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPP 364
             S  V  A+++ +E+I   + +K E+R  + +LL+         + G+L IPTV  PPP
Sbjct: 347 RFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLK---------EDGILVIPTVADPPP 406

Query: 365 KLNTDVSE-LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 424
           +LNT  ++ L++F  + ++L  IA +SG CQV+IPLG +   P+S+SL+  +G D FLL+
Sbjct: 407 RLNTKRNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLD 466

Query: 425 VVHSLYNTLEEEQWI--NLSSLDD 433
               +Y +L+++  +  NL+ + D
Sbjct: 467 TTLDVYASLQDQAKLASNLAPVSD 480

BLAST of MELO3C015328.1 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 92.4 bits (228), Expect = 9.7e-19
Identity = 58/149 (38.93%), Postives = 75/149 (50.34%), Query Frame = 0

Query: 24  PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTVSTILRGGATCIGRTIMDEM 83
           PL G+   VKD     G  +   +       PP + TA  V  I   G   +G+T MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDATA--VKKIKELGGIVVGKTNMDEF 166

Query: 84  AYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV---------GTDTGGSVRVPASYCG 143
                 E   +    NP    RVPGGSS GSA AV         G+DTGGSVR PAS+CG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCG 226

Query: 144 ILGFRPSHGAVSTSGVVPMAQSFDTVGWF 164
           ++G +P++G VS  G++  A S D +G F
Sbjct: 227 VVGLKPTYGRVSRFGLMAYASSLDVIGCF 253

BLAST of MELO3C015328.1 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 83.6 bits (205), Expect = 4.5e-16
Identity = 50/146 (34.25%), Postives = 71/146 (48.63%), Query Frame = 0

Query: 25  LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTVSTILRGGATCIGRTIMDEMA 84
           L G+   +KD  D   H T  G   WL         +  VS +   GA  +G+  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 85  YSINGENFHYGTPQNPCASDRVPGGSSSGSA---------VAVGTDTGGSVRVPASYCGI 144
               G N +YGT +NP    R  GGSSSGSA          A+GTD GGSVR+P++ CGI
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGI 316

Query: 145 LGFRPSHGAVSTSGVVPMAQSFDTVG 162
            G + ++G    +G +    + + +G
Sbjct: 317 TGLKTTYGRTDMTGSLCEGGTVEIIG 341