MELO3C013594.1 (mRNA) Melon (DHL92) v4

BLAST of MELO3C013594.1 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 9.2e-12
Identity = 47/157 (29.94%), Postives = 85/157 (54.14%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGF       + K K  +K    R+++LKNKK+ I +  + ++ +LL     ++A  RV
Sbjct: 1   MFGFG--PSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           E I++DE  +  ++I++  CE +   ++ I    E P ++ E++ +L+++S R   +PEL
Sbjct: 61  ETIIRDEYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLS 158
           + I+     +YG+  E  A   +    VN +I  KLS
Sbjct: 121 EQIKNQLKAKYGKGLENEA-NCHCSTHVNPKIVHKLS 153

BLAST of MELO3C013594.1 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-10
Identity = 45/167 (26.95%), Postives = 90/167 (53.89%), Query Frame = 0

Query: 11  KASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRVEQIVKDETRM 70
           KA + +  ++ V  RLKLL+ KK+ + ++ R++I   L  G  + A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 71  AAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARC-GDLPELQLIRKLFGE 130
            A EIL+ +C+ +L     I+  KE  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 131 RYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRD 177
           +Y + +       N    VN ++  KLS+        ++ + EI ++
Sbjct: 127 KYSKEYGKLC-RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MELO3C013594.1 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-10
Identity = 45/167 (26.95%), Postives = 90/167 (53.89%), Query Frame = 0

Query: 11  KASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRVEQIVKDETRM 70
           KA + +  ++ V  RLKLL+ KK+ + ++ R++I   L  G  + A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 71  AAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARC-GDLPELQLIRKLFGE 130
            A EIL+ +C+ +L     I+  KE  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 131 RYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRD 177
           +Y + +       N    VN ++  KLS+        ++ + EI ++
Sbjct: 127 KYSKEYGKLC-RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MELO3C013594.1 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-10
Identity = 45/167 (26.95%), Postives = 90/167 (53.89%), Query Frame = 0

Query: 11  KASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRVEQIVKDETRM 70
           KA + +  ++ V  RLKLL+ KK+ + ++ R++I   L  G  + A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 71  AAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARC-GDLPELQLIRKLFGE 130
            A EIL+ +C+ +L     I+  KE  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 131 RYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRD 177
           +Y + +       N    VN ++  KLS+        ++ + EI ++
Sbjct: 127 KYSKEYGKLC-RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MELO3C013594.1 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-10
Identity = 45/167 (26.95%), Postives = 90/167 (53.89%), Query Frame = 0

Query: 11  KASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRVEQIVKDETRM 70
           KA + +  ++ V  RLKLL+ KK+ + ++ R++I   L  G  + A  RVE I++++  +
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 71  AAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARC-GDLPELQLIRKLFGE 130
            A EIL+ +C+ +L     I+  KE  + + E+VS+L++A+ R   ++ EL+++      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 131 RYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRD 177
           +Y + +       N    VN ++  KLS+        ++ + EI ++
Sbjct: 127 KYSKGYGKLC-RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MELO3C013594.1 vs. NCBI nr
Match: XP_008448268.1 (PREDICTED: uncharacterized protein LOC103490509 [Cucumis melo])

HSP 1 Score: 964.9 bits (2493), Expect = 2.6e-277
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV
Sbjct: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE
Sbjct: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
           CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE
Sbjct: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK
Sbjct: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY
Sbjct: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT
Sbjct: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480

Query: 481 LKT 484
           LKT
Sbjct: 481 LKT 483

BLAST of MELO3C013594.1 vs. NCBI nr
Match: XP_004140030.2 (uncharacterized protein LOC101206370 isoform X1 [Cucumis sativus] >KGN46752.1 hypothetical protein Csa_020703 [Cucumis sativus])

HSP 1 Score: 879.8 bits (2272), Expect = 1.1e-251
Identity = 441/482 (91.49%), Postives = 457/482 (94.81%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGF FFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLL+NGYHQ AFNRV
Sbjct: 1   MFGFTFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLQNGYHQIAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQIVKDETRM+AYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  EQIVKDETRMSAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIK+KLSIN VSDDEKQ+MMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKQKLSINYVSDDEKQRMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           E+LALEYRSEWHQNQVTAKEVIQVHA+EK +Q KK A+NAYETKKGD+HYS+SVTSTSCE
Sbjct: 181 EVLALEYRSEWHQNQVTAKEVIQVHAEEKIKQHKKQAMNAYETKKGDIHYSNSVTSTSCE 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
            FPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREIS+ENH NQIDLVQSE
Sbjct: 241 FFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISKENHQNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENS S+TSIEGSKKRF+ VVEG PKKEDYK E SSWKQR MDKYW SASE+TTDK
Sbjct: 301 SWSEDENSSSRTSIEGSKKRFMEVVEGNPKKEDYKQENSSWKQRTMDKYWASASEVTTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKP RRRRTKR D PPSHD KFTT DGFNANINHKK EANC K+DVKKDGL 
Sbjct: 361 EIEWANFYKKPRRRRRTKRGDTPPSHDMKFTTDDGFNANINHKKVEANCEKVDVKKDGLC 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKE SFGRTKSCPY+QPSHVHPKLPDYDDIAAKFIALKRERLQ NT
Sbjct: 421 LRAVTMPTERPKERLKEGSFGRTKSCPYKQPSHVHPKLPDYDDIAAKFIALKRERLQYNT 480

Query: 481 LK 483
           LK
Sbjct: 481 LK 482

BLAST of MELO3C013594.1 vs. NCBI nr
Match: KAA0062114.1 (Ist1 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 449/483 (92.96%), Postives = 454/483 (94.00%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV
Sbjct: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
                       + +   F   ++   +      ECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  P---------PKHYLSIGFLLLLVQIKNPTFNILECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE
Sbjct: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
           CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE
Sbjct: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK
Sbjct: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKPM RRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY
Sbjct: 361 EIEWANFYKKPM-RRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT
Sbjct: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 473

Query: 481 LKT 484
           LKT
Sbjct: 481 LKT 473

BLAST of MELO3C013594.1 vs. NCBI nr
Match: TYK04912.1 (IST1 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 846.3 bits (2185), Expect = 1.3e-241
Identity = 434/483 (89.86%), Postives = 434/483 (89.86%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV
Sbjct: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           ELLALEYRSEWHQN                                              
Sbjct: 181 ELLALEYRSEWHQN---------------------------------------------- 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
              QLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE
Sbjct: 241 ---QLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK
Sbjct: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY
Sbjct: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT
Sbjct: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 434

Query: 481 LKT 484
           LKT
Sbjct: 481 LKT 434

BLAST of MELO3C013594.1 vs. NCBI nr
Match: XP_038901982.1 (uncharacterized protein LOC120088637 [Benincasa hispida])

HSP 1 Score: 772.3 bits (1993), Expect = 2.4e-219
Identity = 398/489 (81.39%), Postives = 429/489 (87.73%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGF FFGWRKASKCKKLIKQVQCRLKLL NKKSVITKQLREDIVQL +NGYHQTAFNRV
Sbjct: 1   MFGFVFFGWRKASKCKKLIKQVQCRLKLLNNKKSVITKQLREDIVQLFQNGYHQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQ++KDETRMAAYEILDNFCEFILLNLSYIRKHK+CPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  EQVLKDETRMAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
            LIRKLFGERYGR+FETTAVELNPGNLVNLQIKEKLSI  VSDD+KQ+M+NEI+RD L+P
Sbjct: 121 HLIRKLFGERYGRNFETTAVELNPGNLVNLQIKEKLSITSVSDDDKQRMINEILRDRLQP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           E LALEYRS+WHQN        QVH DEK EQ KK A+NA ETKK DLHYS+SVTSTS E
Sbjct: 181 EFLALEYRSDWHQN--------QVHDDEKAEQHKKQAVNADETKKEDLHYSNSVTSTSSE 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
           C PQ PEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLS REIS+EN+HNQID++QSE
Sbjct: 241 CLPQFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSKREISKENYHNQIDVIQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKED------YKHEKSSWKQRKMDKYWTSAS 360
           SWS++ENS SKTS+EGSKKRF  V EGKPK E+        HEKS WKQ K + YW  AS
Sbjct: 301 SWSDNENSSSKTSVEGSKKRF--VTEGKPKTENDNDIEKDNHEKSPWKQGKRENYW--AS 360

Query: 361 EITTDKEIEWANFYKKP-MRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKID 420
           E TTDKE EWANFYKKP  RRRR K  + PPS D KFTTYDG+N++IN+KK EANC+K+D
Sbjct: 361 EETTDKEKEWANFYKKPRRRRRRRKSRESPPSSDLKFTTYDGYNSSINNKKVEANCQKMD 420

Query: 421 VKKDGLYLRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKR 480
           VKK+GLYLRAVTMP ERPKER KEVSF RT SCPY+QPSHVHPKLPDYDDIAAKFIALKR
Sbjct: 421 VKKEGLYLRAVTMPRERPKERQKEVSFARTISCPYKQPSHVHPKLPDYDDIAAKFIALKR 477

Query: 481 ERLQNNTLK 483
           ERLQNNTLK
Sbjct: 481 ERLQNNTLK 477

BLAST of MELO3C013594.1 vs. ExPASy TrEMBL
Match: A0A1S3BJB1 (uncharacterized protein LOC103490509 OS=Cucumis melo OX=3656 GN=LOC103490509 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.2e-277
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV
Sbjct: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE
Sbjct: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
           CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE
Sbjct: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK
Sbjct: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY
Sbjct: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT
Sbjct: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480

Query: 481 LKT 484
           LKT
Sbjct: 481 LKT 483

BLAST of MELO3C013594.1 vs. ExPASy TrEMBL
Match: A0A0A0KEA6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G128620 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 5.2e-252
Identity = 441/482 (91.49%), Postives = 457/482 (94.81%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGF FFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLL+NGYHQ AFNRV
Sbjct: 1   MFGFTFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLQNGYHQIAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQIVKDETRM+AYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  EQIVKDETRMSAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIK+KLSIN VSDDEKQ+MMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKQKLSINYVSDDEKQRMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           E+LALEYRSEWHQNQVTAKEVIQVHA+EK +Q KK A+NAYETKKGD+HYS+SVTSTSCE
Sbjct: 181 EVLALEYRSEWHQNQVTAKEVIQVHAEEKIKQHKKQAMNAYETKKGDIHYSNSVTSTSCE 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
            FPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREIS+ENH NQIDLVQSE
Sbjct: 241 FFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISKENHQNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENS S+TSIEGSKKRF+ VVEG PKKEDYK E SSWKQR MDKYW SASE+TTDK
Sbjct: 301 SWSEDENSSSRTSIEGSKKRFMEVVEGNPKKEDYKQENSSWKQRTMDKYWASASEVTTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKP RRRRTKR D PPSHD KFTT DGFNANINHKK EANC K+DVKKDGL 
Sbjct: 361 EIEWANFYKKPRRRRRTKRGDTPPSHDMKFTTDDGFNANINHKKVEANCEKVDVKKDGLC 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKE SFGRTKSCPY+QPSHVHPKLPDYDDIAAKFIALKRERLQ NT
Sbjct: 421 LRAVTMPTERPKERLKEGSFGRTKSCPYKQPSHVHPKLPDYDDIAAKFIALKRERLQYNT 480

Query: 481 LK 483
           LK
Sbjct: 481 LK 482

BLAST of MELO3C013594.1 vs. ExPASy TrEMBL
Match: A0A5A7V482 (Ist1 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G004700 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.8e-251
Identity = 449/483 (92.96%), Postives = 454/483 (94.00%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV
Sbjct: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
                       + +   F   ++   +      ECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  P---------PKHYLSIGFLLLLVQIKNPTFNILECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE
Sbjct: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
           CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE
Sbjct: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK
Sbjct: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKPM RRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY
Sbjct: 361 EIEWANFYKKPM-RRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT
Sbjct: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 473

Query: 481 LKT 484
           LKT
Sbjct: 481 LKT 473

BLAST of MELO3C013594.1 vs. ExPASy TrEMBL
Match: A0A5D3C156 (IST1 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001180 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 6.4e-242
Identity = 434/483 (89.86%), Postives = 434/483 (89.86%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV
Sbjct: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL
Sbjct: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP
Sbjct: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           ELLALEYRSEWHQN                                              
Sbjct: 181 ELLALEYRSEWHQN---------------------------------------------- 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
              QLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE
Sbjct: 241 ---QLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK
Sbjct: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420
           EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY
Sbjct: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKDGLY 420

Query: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 480
           LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT
Sbjct: 421 LRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQNNT 434

Query: 481 LKT 484
           LKT
Sbjct: 481 LKT 434

BLAST of MELO3C013594.1 vs. ExPASy TrEMBL
Match: A0A6J1I3N6 (uncharacterized protein LOC111469611 OS=Cucurbita maxima OX=3661 GN=LOC111469611 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 8.6e-154
Identity = 312/497 (62.78%), Postives = 362/497 (72.84%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MFG  FFGWRKASKCKKLIKQVQCRLKLLKNKK +ITKQ++ED+VQL+ NGY QTAFNRV
Sbjct: 1   MFGI-FFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRV 60

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           EQIVKDE R+AAYEILDNFCEFILLNLSYIRKHK+CPNDVNEAVSSL+FA+ARCGDLPEL
Sbjct: 61  EQIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPEL 120

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKP 180
           Q IRKLFGERYGR FE+ AV+L PGNLVN QIKEKL IN VSDD+KQ+MMNEI RDCL+P
Sbjct: 121 QHIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINPVSDDDKQRMMNEIARDCLQP 180

Query: 181 ELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSCE 240
           ELLAL+YRS+WHQNQV A+      ADEK +Q               ++ ++S TS+SC+
Sbjct: 181 ELLALQYRSDWHQNQVVAR---IAKADEKPDQH--------------INQANSGTSSSCD 240

Query: 241 CFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENHHNQIDLVQSE 300
             PQ PEERIVYLDDVVELCSST  EGDQRLFKFKT  TL   EI+ ++  NQ D   +E
Sbjct: 241 SLPQFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEIT-QHRSNQTDTDPTE 300

Query: 301 SWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEITTDK 360
           S ++ EN  SK S++GS         G   K   K E  +  Q        + SE  TDK
Sbjct: 301 SRTDIENPSSKISVKGSM--------GTEGKHQIKGENFTTVQ--------NGSEGATDK 360

Query: 361 EIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHK--------KAEANCRKI 420
           E+EWA+FYKKP  RRRTKR + P S D   TTYD F  N N K         A  NC ++
Sbjct: 361 EMEWASFYKKP-GRRRTKRRELPSSSDLSSTTYDVFTYNGNKKVAANDTKSNAMENCEEM 420

Query: 421 DV------KKDGLYLRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAA 480
            +      K++ LYLRA TMP ERPKE  K V+F RT SCP ++P+HVHPKLPDYDDIAA
Sbjct: 421 KLSSSLRNKREALYLRAETMPPERPKESQK-VNFCRTNSCPDKKPTHVHPKLPDYDDIAA 460

Query: 481 KFIALKRERLQNNTLKT 484
           KF+AL+R+ LQNNTLK+
Sbjct: 481 KFMALRRQHLQNNTLKS 460

BLAST of MELO3C013594.1 vs. TAIR 10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-58
Identity = 165/480 (34.38%), Postives = 238/480 (49.58%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRV 60
           MF F  FGWR+ SKCK ++KQ+QCRL LLKNKK  I+  LR DI QLL  G    A +R 
Sbjct: 71  MFCF-LFGWRRTSKCKSVVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRA 130

Query: 61  EQIVKDETRMAAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPEL 120
           +Q+  DE  M+ Y +L +F + ILLNLSYIR+ ++ P+ +NEAVS+L+FASARCGDLPEL
Sbjct: 131 QQLFLDENLMSLYHLLLHFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPEL 190

Query: 121 QLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDC-LK 180
           + +R LFG+RYG  F  TA+ L PGN VN Q+ EKLSI  VSDD K K++ EIV +  L+
Sbjct: 191 RALRVLFGKRYGNHFVDTALNLLPGNCVNPQVIEKLSIISVSDDAKSKLLGEIVEEYNLR 250

Query: 181 PELLALEYRSEWHQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKGDLHYSDSVTSTSC 240
            E+LA+EY  E+H+         QV   EK E++K++                 ++S S 
Sbjct: 251 LEVLAIEYTPEFHK---------QVLKSEKAEEEKEV-----------------MSSNSA 310

Query: 241 ECFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPT--LSNREISRENHHNQIDLV 300
           + +                 CSS   E +  ++KF  T      N+E  R+    + D +
Sbjct: 311 QPY-----------------CSSQKAESEAEVYKFTLTDADIEENQEQERKVCDEEDDCI 370

Query: 301 QSESWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYWTSASEIT 360
           + E   ED++                V   +   ED + E+   +++      TS+S   
Sbjct: 371 EEEVVEEDQS----------------VFRFRESTEDERKERKRLRRKPRS---TSSSSSP 430

Query: 361 TDKEIEWANFYKKPMRRRRTKREDPPPSHDKKFTTYDGFNANINHKKAEANCRKIDVKKD 420
             K+++   +Y K  R R+ K       H      Y+ F    + K++E   R       
Sbjct: 431 IAKDVDCWRYYYKGKRSRQRKECGKCCYH----IVYNVFTMLPDQKESEEGERS------ 447

Query: 421 GLYLRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDIAAKFIALKRERLQ 478
                                         +++  HVHPKLPDYD I A F AL++++ Q
Sbjct: 491 ------------------------------FKKAMHVHPKLPDYDQIVAHFTALRKQQQQ 447

BLAST of MELO3C013594.1 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 143.7 bits (361), Expect = 4.0e-34
Identity = 128/491 (26.07%), Postives = 224/491 (45.62%), Query Frame = 0

Query: 12  ASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRVEQIVKDETRMA 71
           +SKCK   K    R+KL++NK+ V+ KQ+R DI  LL++G   TA  RVE +++++   A
Sbjct: 28  SSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQA 87

Query: 72  AYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPELQLIRKLFGERY 131
           A EI++ FCE I+  L+ I K K+CP D+ E ++SL+FA+ RC ++PEL  +R +F ++Y
Sbjct: 88  ANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKY 147

Query: 132 GRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRDCLKPELLALEYRSEW 191
           G+ F + A +L P   VN  + +KLS+     + K K+M EI          A E++ +W
Sbjct: 148 GKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEI----------AKEFQVDW 207

Query: 192 HQNQVTAKEVIQVHADEKTEQQKKLAINAYETKKG---------------------DLHY 251
              + T +E+++   +     +K ++ ++    +                      + HY
Sbjct: 208 DTTE-TEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHY 267

Query: 252 SDSVTSTSCECFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSNREISRENH 311
            D  T ++ E   +L ++ +      V    +T  +   + F   +  +   ++    +H
Sbjct: 268 HD--TESAAEAATELAKQAVAAAQ--VASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDH 327

Query: 312 HNQIDLVQSESWSEDENSRSKTSIEGSKKRFVVVVEGKPKKEDYKHEKSSWKQRKMDKYW 371
           H+      S   S D  + S  +  G++ R      G  ++  Y +   +          
Sbjct: 328 HHHHP--GSRRQSRDSETSSYYAKPGAENR------GMGRRHSYNNPGIN---------- 387

Query: 372 TSASEITTDKEIEWANF---YKKPMRRRRT--KREDPPPSHDK-KFTTYDGFNANINHKK 431
                  +D E E+ N     K+ MRRR +   R  PPP+  + KF   D +        
Sbjct: 388 ------ESDYEEEYTNTEAEAKETMRRRHSYNPRSVPPPATSEIKFDESDYYEEE----- 447

Query: 432 AEANCRKIDVKKDGLYLRAVTMPTERPKERLKEVSFGRTKSCPYEQPSHVHPKLPDYDDI 476
                 + D    G   R  ++P  R   +  +    R  S  ++    VHPKLPDYD +
Sbjct: 448 -----TEPDEPSQG---RVSSLPPNRAPPQAPQSGESRQDSSGHQ----VHPKLPDYDIL 462

BLAST of MELO3C013594.1 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 143.3 bits (360), Expect = 5.2e-34
Identity = 74/166 (44.58%), Postives = 106/166 (63.86%), Query Frame = 0

Query: 11  KASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRVEQIVKDETRM 70
           KA+KCK L+K    R+KL++N++    KQ+R +I +LLE G   TA  RVE I+++E  M
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 71  AAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPELQLIRKLFGER 130
           AA EIL+ FCE I + L  I   +ECP D+ EA+SS+ FA+ RC DL ELQ ++ LF  +
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 131 YGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRD 177
           YG+ F   A EL P + VN ++ E LS+   S + K K++ EI  +
Sbjct: 133 YGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEE 178

BLAST of MELO3C013594.1 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 139.8 bits (351), Expect = 5.7e-33
Identity = 73/166 (43.98%), Postives = 104/166 (62.65%), Query Frame = 0

Query: 11  KASKCKKLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNRVEQIVKDETRM 70
           K +KCK  ++    RLK+LKNKK +  KQLR ++ QLLE+G   TA  RVE +V++E  +
Sbjct: 10  KPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEKTV 69

Query: 71  AAYEILDNFCEFILLNLSYIRKHKECPNDVNEAVSSLLFASARCGDLPELQLIRKLFGER 130
           AAYE++  +CE +++ L  I   K CP D+ EAV+S+LFAS R  D+PEL  I K F  +
Sbjct: 70  AAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFTTK 129

Query: 131 YGRSFETTAVELNPGNLVNLQIKEKLSINCVSDDEKQKMMNEIVRD 177
           YG+ F T+AVEL P + V+  + EKLS        K K++  I  +
Sbjct: 130 YGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEE 175

BLAST of MELO3C013594.1 vs. TAIR 10
Match: AT1G51900.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 131.3 bits (329), Expect = 2.0e-30
Identity = 113/376 (30.05%), Postives = 192/376 (51.06%), Query Frame = 0

Query: 1   MFGFAFFGWRKASKCK-KLIKQVQCRLKLLKNKKSVITKQLREDIVQLLENGYHQTAFNR 60
           MFGF+F   RK++    K++KQ+Q RL LLK++K   ++ LR DIV  + +   ++A  R
Sbjct: 1   MFGFSFSRRRKSNAFTWKVLKQLQSRLMLLKSQKYAKSRHLRADIVDFIRSNDSKSALFR 60

Query: 61  VEQIVKDETRMAAYEILDNFCEFILLNLSYIRKHK--ECPNDVNEAVSSLLFASARCGDL 120
            EQ++  E  +  Y  L  F +FILL  S  +KH      +D +EAVSSL+FAS +C ++
Sbjct: 61  TEQLLLVENAITIYGFLLKFTDFILLRFSPSKKHSCLLVNDDTSEAVSSLIFASVKCREI 120

Query: 121 PELQLIRKLFGERYGRSFETTAVELNPGNLVNLQIKEKL-SINCVSDDEKQKMMNEIVRD 180
           PEL +I +L G+RYG+ + TTA+++ PGNLVN +IKEKL S + VS+ +K ++M EI ++
Sbjct: 121 PELLIISELVGQRYGQRYVTTAIQVPPGNLVNTEIKEKLKSTSVVSETDKCRVMEEIAKE 180

Query: 181 C-LKPELLALEYRSE-------WHQNQVTAKEVIQVH------ADEKTEQQKKLAINAYE 240
              + E+L L Y+SE         +  V  + + +V+       DE+++++  +  +  E
Sbjct: 181 SGYRLEILGLGYKSEIDNEVFDLEEKNVMDESLHEVYKFCLTDVDERSKKETSMKDDYIE 240

Query: 241 TKKGDLHYSDSVTSTSCECFPQLPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSN 300
            KK     +++   +        PE +     +V E       + D  +  ++ +P    
Sbjct: 241 HKKSTRLLAENAKKSGHSLEILRPESKPETEKEVNEEEEKRVMDPDVDISCYEESP---- 300

Query: 301 REISRENHHNQIDLVQSESWSEDENSRSKTSIEGSKKRFVVVVEG---KPKKEDYKHEKS 356
            E+ + +  +  + +  + + ED   R    I  +    V +      KP+ E   +EK 
Sbjct: 301 HEVYKFSLTDFEEEIMEDDYREDMKCRMLDDIVKNSGHRVEISRPEYYKPEIEKQVYEKE 360