MELO3C004433.jh1.t3 (mRNA) Melon (Harukei-3) v1.41

Overview
NameMELO3C004433.jh1.t3
TypemRNA
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptioncalcium uptake protein, mitochondrial-like
Locationchr05: 27809555 .. 27812397 (-)
Sequence length1729
RNA-Seq ExpressionMELO3C004433.jh1.t3
SyntenyMELO3C004433.jh1.t3
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCATTACCTTTTTCTTGATGATATTTGGAAGAACCTCAAGGTAGATCTAAAGCATAGTATGTTTTCTTCGACGTTTTTGGTTTCAAAATCTTCTAAGTCGATCTGTAGAGTTATACCTTCTCAACACCTTACTGTTCGTTTTCTCAACAATCAACTTGGTGGCTCTTTAGGATCGCCATCAACATCCCCATTGTCTTCTTCTTCGTCGATCACAACTCAACAAACTGGCTGCGGAGGCGAGAATAGAAGAAACTTTGTATTTGAGTTCTTGATCAAATTGATTTCTTCAGGGTTATTGATTGGCGGAGTGGGATCACTTCTCAGCTCCAAGTCTTCCACGTCCAATTCGCATTTATCGTTTGCTGACTTCCCTAAAGAAACAACATGGACAACAGTGAAAGAAGATCAATTCCAATACCCATCACCAAATCAAAATATAAATGCAAAGAAGAAATCCAAATTTCTATTTGGAGGTAAGAATGTCCCCTTTTTGCCATTAAACTTGAAGCAATCTAAGTTAAATGTGTTTAGGGTAAAGTTACCAATTTATTCACACTTTATGCTGGATGCCAGATGATTATAGGAGAAGGGTGTTTTTCAACTATGAAAAACGTATAAGAATCCAAAGTCCTCCTGAGAAGGTGTTTGAATATTTTGCCTCTATTCACACACCTGAAGGGGAAATATATATGAGACCATCAGATCTGATGAGGGCCATTGTTCCTGTATTTCCTCCATCTGAGTCCAATCGTGTTAGAGAAGGCTTTCTAAGAGGAGAAAGAAGAATTTCTGGAGAACTATGTTGTGCTCCTTCCAAGTTTTTCATGCTTTTCGACACCAACAATGATGGCCTCATCTCTTTTGCAGAGTAAGAATTCGACCCTTCTTCCAACTCCATGATAATTTGCACAACCTAACATTCACATATAGTTGAACCTCATTCAAACTTAAAATAAATTTCTAATCAATGCATATAACATTTTGTTTCTAACTCTCAACATTAATTCTAAACTTTGTCTTATTGGTCTCAAAATAAATTATTTATTTATTTCGGGTGAGCTATCTAATAAATTGTTGAACTTTAACAAGGTTAAAGTATTTATCAACTTTGTTTATATGCCCAAACCTATTGGATCAAAACCAAAGGTTGGAAATTCATGCATTGTGGACTCGTCTTCGGTTTGCAGGTACATCTTTTTCGTCACACTTCTCAGCATACCAGAATCCAGCTTTTCAGTGGCTTTCAAAATGTTTGACATTGATAACAATGGGTTAGATAAATAATTGCTCCTTCAAATTCATCATTTTCCACGTTCGTTTCCTCTTCTCTTCACAATCCTCTAGGGTAATGGATGTTATCTTTGTCTTTGTTGATAAAAATAGTGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGTTTGATGAGAAAACAGAACAGACAGGGAGCTCACCACAGAGATGGAAGAAGGTTTGGGATGAAAGTTAGTGTTGAAAATGGTGGTTTAGTAGAATACTTCTTTGGTGAAGACGGAAAAGGCTCACTTCACCATGACAAATTTGTCCAATTCCTTAGACAACTTCATGAAGAGGTATGTCATTCCTCCCAATATCAATTCTTCAAATACTTGAACTCTTTGGAACTCAAAGACACAATTGTACAACCTATAAAACAAAACCTGTTCAAATTCAGAACAGTTCCTATTCAAATTCAAGTAAGGTATTTCTTCTTATACTCAACTCTTTAGAACTCAAGGCACGGCTGTACAGCCTATAGAACAGATGTATTGAAATCCAAGTACAACTGTTCTTCCTGTACTTAACTCTTTAAACTCAAAGATTACGGGTGTACAAGCTATAGAACAGATCTATTCAAATTCATGTAAGGTTCCTCTTCCTATACTTGTTAGACTCTTTATACTCAAAGACACGATTGTACAATCTATACAATAGATCCCTATTTAAATCCTTCCAGTACTGAACTCTTTAGAAATAAAATACACGGTTGTAGAAACTCTAAAGCAGATCCTATTCAAATTCACGCAAGGTCTTCTTCCTACACTGACTCAACCTACATAAATTGGATGAATGCAGATACTTATACTAGAGTTTTCACATTACGACTTCAAATCACAAGGAAGCATATCAGTCAAAGACTTTGCACTATCAATAGTTGCTTCAGCAGATATAAATCACATAGACAAACTACTGGACAGAGTTGAAGCATTAAACAAAGAACCCCATTTCAAAAACATCAGAATCACATATGAAGAATTCAAAGACTTTGCAGAGCTACGCAAGAAATTAGAATCCTTCTCTTTGGCTATTTTCAGCTATGGTAAAGTAAATGGAGAATTAACAAAACAAGATTTCCAACGAGCAGCTTCCCAAGTAAGTACACACCAAACATTTCAAGTCAACAAACTTAAAATCAATCTAATACATAATGTGGGTTTTGTTTTTCTGTACAAGGTGTGTGGAGTTTCAATCACTAAGAATGTTGTTGACATAATATTTCACATATTTGATGCAAATGAAGATGGGGATTTAAGCTCAGATGAGTTCGTAAGAGTCATACAGAGAAGAGAAGTGAATTCTTCACAACCTGCTGTTGGTGTTGGAGGACTACTGTCTTGTTGGTTTAGCTGTGCAGCTAAATGTTCTTCCGCAAAGCTCTTTGTTCGCAGCTGAAGAATTTGTTGCCCTAATGATGTTCCTGTAAACATGTAAATTTTCCGACATGCTGATATACTTTCTAGTGGACTATGTTCTTTAGGGATATATTCATTTACAAGATTGATCTTCGATTTTAAGTTCAATCAAAATAAATAAATTACAAAAC

mRNA sequence

TTCCATTACCTTTTTCTTGATGATATTTGGAAGAACCTCAAGGTAGATCTAAAGCATAGTATGTTTTCTTCGACGTTTTTGGTTTCAAAATCTTCTAAGTCGATCTGTAGAGTTATACCTTCTCAACACCTTACTGTTCGTTTTCTCAACAATCAACTTGGTGGCTCTTTAGGATCGCCATCAACATCCCCATTGTCTTCTTCTTCGTCGATCACAACTCAACAAACTGGCTGCGGAGGCGAGAATAGAAGAAACTTTGTATTTGAGTTCTTGATCAAATTGATTTCTTCAGGGTTATTGATTGGCGGAGTGGGATCACTTCTCAGCTCCAAGTCTTCCACGTCCAATTCGCATTTATCGTTTGCTGACTTCCCTAAAGAAACAACATGGACAACAGTGAAAGAAGATCAATTCCAATACCCATCACCAAATCAAAATATAAATGCAAAGAAGAAATCCAAATTTCTATTTGGAGATGATTATAGGAGAAGGGTGTTTTTCAACTATGAAAAACGTATAAGAATCCAAAGTCCTCCTGAGAAGGTGTTTGAATATTTTGCCTCTATTCACACACCTGAAGGGGAAATATATATGAGACCATCAGATCTGATGAGGGCCATTGTTCCTGTATTTCCTCCATCTGAGTCCAATCGTGTTAGAGAAGGCTTTCTAAGAGGAGAAAGAAGAATTTCTGGAGAACTATGTTGTGCTCCTTCCAAGTTTTTCATGCTTTTCGACACCAACAATGATGGCCTCATCTCTTTTGCAGAGTACATCTTTTTCGTCACACTTCTCAGCATACCAGAATCCAGCTTTTCAGTGGCTTTCAAAATGTTTGACATTGATAACAATGGTGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGTTTGATGAGAAAACAGAACAGACAGGGAGCTCACCACAGAGATGGAAGAAGGTTTGGGATGAAAGTTAGTGTTGAAAATGGTGGTTTAGTAGAATACTTCTTTGGTGAAGACGGAAAAGGCTCACTTCACCATGACAAATTTGTCCAATTCCTTAGACAACTTCATGAAGAGATACTTATACTAGAGTTTTCACATTACGACTTCAAATCACAAGGAAGCATATCAGTCAAAGACTTTGCACTATCAATAGTTGCTTCAGCAGATATAAATCACATAGACAAACTACTGGACAGAGTTGAAGCATTAAACAAAGAACCCCATTTCAAAAACATCAGAATCACATATGAAGAATTCAAAGACTTTGCAGAGCTACGCAAGAAATTAGAATCCTTCTCTTTGGCTATTTTCAGCTATGGTAAAGTAAATGGAGAATTAACAAAACAAGATTTCCAACGAGCAGCTTCCCAAGTGTGTGGAGTTTCAATCACTAAGAATGTTGTTGACATAATATTTCACATATTTGATGCAAATGAAGATGGGGATTTAAGCTCAGATGAGTTCGTAAGAGTCATACAGAGAAGAGAAGTGAATTCTTCACAACCTGCTGTTGGTGTTGGAGGACTACTGTCTTGTTGGTTTAGCTGTGCAGCTAAATGTTCTTCCGCAAAGCTCTTTGTTCGCAGCTGAAGAATTTGTTGCCCTAATGATGTTCCTGTAAACATGTAAATTTTCCGACATGCTGATATACTTTCTAGTGGACTATGTTCTTTAGGGATATATTCATTTACAAGATTGATCTTCGATTTTAAGTTCAATCAAAATAAATAAATTACAAAAC

Coding sequence (CDS)

ATGTTTTCTTCGACGTTTTTGGTTTCAAAATCTTCTAAGTCGATCTGTAGAGTTATACCTTCTCAACACCTTACTGTTCGTTTTCTCAACAATCAACTTGGTGGCTCTTTAGGATCGCCATCAACATCCCCATTGTCTTCTTCTTCGTCGATCACAACTCAACAAACTGGCTGCGGAGGCGAGAATAGAAGAAACTTTGTATTTGAGTTCTTGATCAAATTGATTTCTTCAGGGTTATTGATTGGCGGAGTGGGATCACTTCTCAGCTCCAAGTCTTCCACGTCCAATTCGCATTTATCGTTTGCTGACTTCCCTAAAGAAACAACATGGACAACAGTGAAAGAAGATCAATTCCAATACCCATCACCAAATCAAAATATAAATGCAAAGAAGAAATCCAAATTTCTATTTGGAGATGATTATAGGAGAAGGGTGTTTTTCAACTATGAAAAACGTATAAGAATCCAAAGTCCTCCTGAGAAGGTGTTTGAATATTTTGCCTCTATTCACACACCTGAAGGGGAAATATATATGAGACCATCAGATCTGATGAGGGCCATTGTTCCTGTATTTCCTCCATCTGAGTCCAATCGTGTTAGAGAAGGCTTTCTAAGAGGAGAAAGAAGAATTTCTGGAGAACTATGTTGTGCTCCTTCCAAGTTTTTCATGCTTTTCGACACCAACAATGATGGCCTCATCTCTTTTGCAGAGTACATCTTTTTCGTCACACTTCTCAGCATACCAGAATCCAGCTTTTCAGTGGCTTTCAAAATGTTTGACATTGATAACAATGGTGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGTTTGATGAGAAAACAGAACAGACAGGGAGCTCACCACAGAGATGGAAGAAGGTTTGGGATGAAAGTTAGTGTTGAAAATGGTGGTTTAGTAGAATACTTCTTTGGTGAAGACGGAAAAGGCTCACTTCACCATGACAAATTTGTCCAATTCCTTAGACAACTTCATGAAGAGATACTTATACTAGAGTTTTCACATTACGACTTCAAATCACAAGGAAGCATATCAGTCAAAGACTTTGCACTATCAATAGTTGCTTCAGCAGATATAAATCACATAGACAAACTACTGGACAGAGTTGAAGCATTAAACAAAGAACCCCATTTCAAAAACATCAGAATCACATATGAAGAATTCAAAGACTTTGCAGAGCTACGCAAGAAATTAGAATCCTTCTCTTTGGCTATTTTCAGCTATGGTAAAGTAAATGGAGAATTAACAAAACAAGATTTCCAACGAGCAGCTTCCCAAGTGTGTGGAGTTTCAATCACTAAGAATGTTGTTGACATAATATTTCACATATTTGATGCAAATGAAGATGGGGATTTAAGCTCAGATGAGTTCGTAAGAGTCATACAGAGAAGAGAAGTGAATTCTTCACAACCTGCTGTTGGTGTTGGAGGACTACTGTCTTGTTGGTTTAGCTGTGCAGCTAAATGTTCTTCCGCAAAGCTCTTTGTTCGCAGCTGA

Protein sequence

MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGGENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS
Homology
BLAST of MELO3C004433.jh1.t3 vs. NCBI nr
Match: XP_008456571.1 (PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X2 [Cucumis melo])

HSP 1 Score: 993 bits (2566), Expect = 0.0
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60
           MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60

Query: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120
           ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY
Sbjct: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120

Query: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180
           PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP
Sbjct: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180

Query: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240
           SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF
Sbjct: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240

Query: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300
           FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS
Sbjct: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300

Query: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360
           VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI
Sbjct: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360

Query: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420
           VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG
Sbjct: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420

Query: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480
           ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV
Sbjct: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480

Query: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505
           GVGGLLSCWFSCAAKCSSAKLFVRS
Sbjct: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of MELO3C004433.jh1.t3 vs. NCBI nr
Match: KAA0063559.1 (calcium uptake protein 1 [Cucumis melo var. makuwa] >TYJ97885.1 calcium uptake protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 988 bits (2555), Expect = 0.0
Identity = 502/505 (99.41%), Postives = 504/505 (99.80%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60
           MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60

Query: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120
           ENRRNFVFEFLIKLISSG+LIGGVGSLLSS SSTSNSHLSFA+FPKETTWTTVKEDQFQY
Sbjct: 61  ENRRNFVFEFLIKLISSGILIGGVGSLLSSNSSTSNSHLSFAEFPKETTWTTVKEDQFQY 120

Query: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180
           PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP
Sbjct: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180

Query: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240
           SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF
Sbjct: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240

Query: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300
           FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS
Sbjct: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300

Query: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360
           VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI
Sbjct: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360

Query: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420
           VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG
Sbjct: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420

Query: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480
           ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV
Sbjct: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480

Query: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505
           GVGGLLSCWFSCAAKCSSAKLFVRS
Sbjct: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of MELO3C004433.jh1.t3 vs. NCBI nr
Match: XP_008456564.1 (PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X1 [Cucumis melo])

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 505/532 (94.92%), Postives = 505/532 (94.92%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60
           MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60

Query: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120
           ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY
Sbjct: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120

Query: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180
           PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP
Sbjct: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180

Query: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240
           SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF
Sbjct: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240

Query: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300
           FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS
Sbjct: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300

Query: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360
           VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI
Sbjct: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360

Query: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420
           VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG
Sbjct: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420

Query: 421 ELTKQDFQRAASQV---------------------------CGVSITKNVVDIIFHIFDA 480
           ELTKQDFQRAASQV                           CGVSITKNVVDIIFHIFDA
Sbjct: 421 ELTKQDFQRAASQVSTHQTFQVNKLKINLIHNVGFVFLYKVCGVSITKNVVDIIFHIFDA 480

Query: 481 NEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505
           NEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS
Sbjct: 481 NEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 532

BLAST of MELO3C004433.jh1.t3 vs. NCBI nr
Match: XP_004139397.1 (calcium uptake protein, mitochondrial [Cucumis sativus] >KGN60757.1 hypothetical protein Csa_019233 [Cucumis sativus])

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 481/511 (94.13%), Postives = 494/511 (96.67%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPL---SSSSSITTQQTG 60
           MFSSTFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPL   SSSSSIT+QQTG
Sbjct: 1   MFSSTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLFSSSSSSSITSQQTG 60

Query: 61  CGG-ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKED 120
           CGG ENRR+FVF+FLIKLISSG+LIGGVGSLLSS SS SNSHLSFADFPKETTWTTVKED
Sbjct: 61  CGGGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED 120

Query: 121 QFQYPS-PN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180
           QFQYPS PN QNIN KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG
Sbjct: 121 QFQYPSSPNHQNINPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180

Query: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLIS 240
           EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERR+SGELCCAPS FFMLFDTNNDGLIS
Sbjct: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRMSGELCCAPSTFFMLFDTNNDGLIS 240

Query: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR 300
           FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHR+GRR
Sbjct: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHREGRR 300

Query: 301 FGMKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVK 360
           FGMKVSVENGGLVEYFFG+DGK SLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS K
Sbjct: 301 FGMKVSVENGGLVEYFFGQDGKASLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK 360

Query: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFS 420
           DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFS
Sbjct: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS 420

Query: 421 YGKVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480
           YGKVNGELTKQDFQRAAS VCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN
Sbjct: 421 YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480

Query: 481 SSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505
           S+QPAVG GGLLSCWF CAAKCS AKLFVRS
Sbjct: 481 STQPAVGAGGLLSCWFGCAAKCSYAKLFVRS 511

BLAST of MELO3C004433.jh1.t3 vs. NCBI nr
Match: XP_022143166.1 (calcium uptake protein, mitochondrial-like [Momordica charantia])

HSP 1 Score: 813 bits (2099), Expect = 1.01e-293
Identity = 425/506 (83.99%), Postives = 455/506 (89.92%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLG-SPSTSPLSSSSSITTQQTGCG 60
           MFSST LVSKSS SI RV+ SQ LTVR  NNQLGGS   SPS+S L+SS       TG G
Sbjct: 1   MFSSTLLVSKSSASIHRVVTSQRLTVRLFNNQLGGSASTSPSSSLLASS-----HNTGSG 60

Query: 61  GENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQ 120
            E++R+ +F+FL+K +SSG++  G+GSLL   SSTSNS++SFADF KET WT +KEDQFQ
Sbjct: 61  EESKRSSIFDFLVKSVSSGVVAVGIGSLLCLNSSTSNSNVSFADFQKETKWT-MKEDQFQ 120

Query: 121 YPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMR 180
           YP PNQN  A+KKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS+HTPEGEI+M 
Sbjct: 121 YPFPNQN--ARKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASVHTPEGEIFMT 180

Query: 181 PSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYI 240
           P+DLMRAIVPVFPPSESNRVREGFLRGER I GEL C PS+FFMLFD N+DGLISFAEYI
Sbjct: 181 PADLMRAIVPVFPPSESNRVREGFLRGER-IHGELYCVPSEFFMLFDINSDGLISFAEYI 240

Query: 241 FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV 300
           FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVM LMRKQNRQGA HRDGRR GMKV
Sbjct: 241 FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMALMRKQNRQGARHRDGRRLGMKV 300

Query: 301 SVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALS 360
            VENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL LEFSHYDFKSQGSIS KDFALS
Sbjct: 301 CVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILRLEFSHYDFKSQGSISAKDFALS 360

Query: 361 IVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVN 420
           +VASADINHIDKLLDRVEALNKEP F+NIRITYEEFKDFAELRKKLESFSLAIFSYGKVN
Sbjct: 361 MVASADINHIDKLLDRVEALNKEPQFRNIRITYEEFKDFAELRKKLESFSLAIFSYGKVN 420

Query: 421 GELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPA 480
           GELTKQDFQRAAS VCGVSIT+NVVDIIFHIFDAN DGDLSSDEFVRVIQR+EV+SSQ A
Sbjct: 421 GELTKQDFQRAASHVCGVSITRNVVDIIFHIFDANGDGDLSSDEFVRVIQRKEVSSSQAA 480

Query: 481 VGVGGLLSCWFSCAAKCSSAKLFVRS 505
           VG GG LSCWFSC+AKCSSAKLFVRS
Sbjct: 481 VGAGGFLSCWFSCSAKCSSAKLFVRS 497

BLAST of MELO3C004433.jh1.t3 vs. ExPASy Swiss-Prot
Match: Q9SZ45 (Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE=2 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 5.6e-137
Identity = 243/370 (65.68%), Postives = 305/370 (82.43%), Query Frame = 0

Query: 133 SKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFP 192
           S F+FGD YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T +GEI M+P+DLMRAIVPVFP
Sbjct: 130 SGFIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFP 189

Query: 193 PSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSF 252
           PSES+ VREG+L GER   GEL C+PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF
Sbjct: 190 PSESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSF 249

Query: 253 SVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV--SVENGGLVEYF 312
           +VAFKMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G+ +  SVE+GGLVEYF
Sbjct: 250 AVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYF 309

Query: 313 FGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHID 372
           FG+DG   L HDKF +F++ L EE+L LEF+HYD+K +GSIS KDFALS+VA+AD +H+ 
Sbjct: 310 FGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLS 369

Query: 373 KLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRA 432
           KLLDRVE+L++ PH +++RI+ +EFK F ELR KL  FSLA+F+YGK NG LT +DF+RA
Sbjct: 370 KLLDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRA 429

Query: 433 ASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQP-AVGVGGLLSCW 492
           ASQVCG++++ NV++I FH+FD+N+DG+LS DEF+RV+ RRE + +QP A G+    S  
Sbjct: 430 ASQVCGITLSDNVIEIAFHVFDSNQDGNLSVDEFLRVLHRRERDVAQPIAKGLSRYFSDG 489

Query: 493 FSCAAKCSSA 500
           +  +  CSS+
Sbjct: 490 WKGSKNCSSS 498

BLAST of MELO3C004433.jh1.t3 vs. ExPASy Swiss-Prot
Match: B1H2N3 (Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 PE=2 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.7e-45
Identity = 124/413 (30.02%), Postives = 209/413 (50.61%), Query Frame = 0

Query: 91  KSSTSNSHLSFADFPKETTWTTVKEDQFQYPSPNQNINAKKKSKFLFGDDYRRRVFFNYE 150
           K + + +  S     +E T    KED  Q    +     ++  K      +R R    YE
Sbjct: 53  KRANAEAQSSVKHSMREETSEKEKEDADQAVESSDEDQPQEGKKKKARVGFRDRKVMEYE 112

Query: 151 KRIRIQSPPEKVFEYFASI----HTPEGEIYMRPSDLMRAIVPVFPPSESNRVREGFLR- 210
            RIR  S P+K+F YFA++     + E E++M P D +R+I P     E+  + +  ++ 
Sbjct: 113 NRIRAYSTPDKIFRYFATLKVIHESGESEVFMTPQDFVRSITPNEKQPENLGLDQFIIKR 172

Query: 211 -GERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNN 270
              ++IS E      +  + +     GLISF++YIF  T+LS P+ +F +AFKMFD++ +
Sbjct: 173 YDGKKISQEREKFADEDSIFYSLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGD 232

Query: 271 GEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVSVE-NGGLVEYFFGEDGKGSLHHDK 330
           GE+D EEF++V  ++R Q   G  HRD    G  +    +  L  YFFG D KG L    
Sbjct: 233 GEVDMEEFEQVQSIIRSQTSMGMRHRDRSTTGNTLKTGFSSALTTYFFGADLKGKLTIKN 292

Query: 331 FVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHIDKLLDRVEALNKEP 390
           F++F R+L  ++L LEF  +D    G I+ + F   ++A + +    KL   ++ L K  
Sbjct: 293 FLEFQRKLQHDVLKLEFERHD-PVDGHITERQFGSMLLAYSGVQS-KKLTHMLKQLKK-- 352

Query: 391 HFKNIR-ITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASQVCGVSITKN 450
            FK+   +T+EE ++F    K +     A+  Y      L K   Q+ A  V  V ++ +
Sbjct: 353 RFKDAEGLTFEEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDH 412

Query: 451 VVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPA--VGVGGLLSCWFSCA 494
           V D++F +FD + +G+LS+ EF+ ++++R +   +    +G   L+   + CA
Sbjct: 413 VCDVVFALFDCDGNGELSNKEFIAIMKQRLMRGLEKPKDMGFTRLMRAMWKCA 461

BLAST of MELO3C004433.jh1.t3 vs. ExPASy Swiss-Prot
Match: A2VEI2 (Calcium uptake protein 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MICU1 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 5.1e-45
Identity = 117/361 (32.41%), Postives = 183/361 (50.69%), Query Frame = 0

Query: 141 YRRRVFFNYEKRIRIQSPPEKVFEYFASIHTP----EGEIYMRPSDLMRAIVPVFPPSES 200
           +R R    YE RIR  S P+KVF YFA+I  P      E+YM P+D + ++ P     + 
Sbjct: 158 FRERKIIEYENRIRQFSTPDKVFRYFATIQVPVADDRHEVYMTPTDFLTSMTPGMKQPDG 217

Query: 201 NRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 260
             + +      + +  +L     K  + +   + GLI+F++YIF +T+LSI    F +AF
Sbjct: 218 LGLDQYRRYDPKSVGEQLNLHLEKNSIFYKLGSYGLITFSDYIFLLTVLSISRRHFEIAF 277

Query: 261 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVSVENGGLVEYFFGEDGK 320
           +MFD++ +G++D EEF+ V  L+R+Q   G  HRD    G      N  L+ YFFG +  
Sbjct: 278 RMFDLNGDGDVDCEEFEMVATLVRQQTSMGTRHRDHANTGNTFKGVNSALITYFFGPNMD 337

Query: 321 GSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASA--DINHIDKLLD 380
             L  +KF+ F  QL  EIL LEF   +   +G+I+  DFA  ++A A   +    K L 
Sbjct: 338 EKLTIEKFLDFQEQLQREILSLEFERKEPNDEGNITEADFAELLLAYAGYPLKKKQKKLK 397

Query: 381 RVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASQV 440
           RV+   ++ H K   I+ +++ DF      +     A+  Y      + +Q  Q  A  V
Sbjct: 398 RVKRRFRD-HGKG--ISKQDYLDFFHFLNNINDVDTALTFYHIAGASIDQQTLQHVAKTV 457

Query: 441 CGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGL--LSCWFSC 494
             V+++ +VVD++F IFD N D  LS+ EF+ V++ R     +     G L  +   F C
Sbjct: 458 AMVNLSDHVVDVVFTIFDENNDNQLSNKEFISVMKNRVQRGLEKPKDTGFLKMMRSVFKC 515

BLAST of MELO3C004433.jh1.t3 vs. ExPASy Swiss-Prot
Match: Q95PZ2 (Calcium uptake protein 1 homolog, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=micu-1 PE=2 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.9e-44
Identity = 123/371 (33.15%), Postives = 190/371 (51.21%), Query Frame = 0

Query: 114 KEDQFQYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASI---- 173
           KE+  +     + +  K K K L    +R R    YE R+R+ S P+K+F YFA++    
Sbjct: 136 KEEVTESNGEVEEVKEKPKKKKL---GFRERRIIEYEDRLRLYSTPDKIFRYFATLKIID 195

Query: 174 ---HTPEGEIYMRPSDLMRAIVP-VFPPSESNRVREGFLRGERRISGELCCAPSKFFMLF 233
               +   E++M P D +R+  P V  P             E+    +     S F+ L 
Sbjct: 196 PNEDSGRFEVFMTPEDFLRSFTPGVMQPRRWGLDSFKNYNPEKHKRHKFSDPDSIFYKL- 255

Query: 234 DTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQ 293
               +GLI+F++Y+F +TLLS   + F++AFK+FD+D NG +D+EEF KV  L+  Q   
Sbjct: 256 --GENGLINFSDYLFLMTLLSTSHADFALAFKIFDVDGNGALDKEEFTKVQQLIMSQTTV 315

Query: 294 GAHHRDGRRFGMKVSVE-NGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYD 353
           G  HRD         VE N  L  YFFG+DGKGSL  +KF++F  +L  +IL +EF   D
Sbjct: 316 GQRHRDHITPNQSFRVETNSALETYFFGKDGKGSLSSEKFIEFQERLQHDILKMEFERRD 375

Query: 354 F--KSQGSISVKDFALSIVASADINHIDK--LLDRVEALNKEPHFKNIRITYEEFKDFAE 413
                 G I+   FA  ++  A IN   +  +L RV+   K  + K   I++ E K F E
Sbjct: 376 ALDNPDGLINEDSFAQLLLLHAQINEKKQKHMLKRVKRRFKGENLKG--ISFGETKAFFE 435

Query: 414 LRKKLESFSLAIFSYGKVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLS 472
               ++   +A+  +      +  +  QR A +V G+ ++ +VVD++  +FD N DG LS
Sbjct: 436 FLYHIDDVDIALHFHKMAGMSIDAKLLQRVAVKVTGIPLSDHVVDVVITLFDDNLDGKLS 495

BLAST of MELO3C004433.jh1.t3 vs. ExPASy Swiss-Prot
Match: Q4R518 (Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 PE=2 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.9e-44
Identity = 124/382 (32.46%), Postives = 199/382 (52.09%), Query Frame = 0

Query: 122 SPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS---IHTP-EGEIY 181
           +P+     KK+S F      R R    YE RIR  S P+K+F YFA+   I+ P E E++
Sbjct: 93  APHPEEKKKKRSGF------RDRKVMEYENRIRAYSTPDKIFRYFATLKVINEPGEAEVF 152

Query: 182 MRPSDLMRAIVPVFPPSESNRVREGFLR--GERRISGELCCAPSKFFMLFDTNNDGLISF 241
           M P D +R+I P     E   + +  ++    ++IS E      +  + +     GLISF
Sbjct: 153 MTPEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISF 212

Query: 242 AEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRF 301
           ++YIF  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    
Sbjct: 213 SDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTT 272

Query: 302 GMKV-SVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVK 361
           G  + S     L  YFFG D KG L    F++F R+L  ++L LEF  +D    G I+ +
Sbjct: 273 GNTLKSGLCSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRITER 332

Query: 362 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIR-ITYEEFKDFAELRKKLESFSLAIF 421
            F   ++A + +    KL      L K  HFK  + +T++E ++F    K +     A+ 
Sbjct: 333 QFGGMLLAYSGVQS-KKLTAMQRQLKK--HFKEGKGLTFQEVENFFTFLKNINDVDTALS 392

Query: 422 SYGKVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREV 481
            Y      L K   Q+ A  V  V ++ +V D++F +FD + +G+LS+ EFV ++++R +
Sbjct: 393 FYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVSIMKQRLM 452

Query: 482 NSSQPA--VGVGGLLSCWFSCA 494
              +    +G   L+   + CA
Sbjct: 453 RGLEKPKDMGFTRLMQAMWKCA 464

BLAST of MELO3C004433.jh1.t3 vs. ExPASy TrEMBL
Match: A0A1S3C4U2 (calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 993 bits (2566), Expect = 0.0
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60
           MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60

Query: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120
           ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY
Sbjct: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120

Query: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180
           PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP
Sbjct: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180

Query: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240
           SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF
Sbjct: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240

Query: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300
           FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS
Sbjct: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300

Query: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360
           VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI
Sbjct: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360

Query: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420
           VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG
Sbjct: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420

Query: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480
           ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV
Sbjct: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480

Query: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505
           GVGGLLSCWFSCAAKCSSAKLFVRS
Sbjct: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of MELO3C004433.jh1.t3 vs. ExPASy TrEMBL
Match: A0A5A7VDG3 (Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001000 PE=4 SV=1)

HSP 1 Score: 988 bits (2555), Expect = 0.0
Identity = 502/505 (99.41%), Postives = 504/505 (99.80%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60
           MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60

Query: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120
           ENRRNFVFEFLIKLISSG+LIGGVGSLLSS SSTSNSHLSFA+FPKETTWTTVKEDQFQY
Sbjct: 61  ENRRNFVFEFLIKLISSGILIGGVGSLLSSNSSTSNSHLSFAEFPKETTWTTVKEDQFQY 120

Query: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180
           PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP
Sbjct: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180

Query: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240
           SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF
Sbjct: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240

Query: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300
           FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS
Sbjct: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300

Query: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360
           VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI
Sbjct: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360

Query: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420
           VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG
Sbjct: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420

Query: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480
           ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV
Sbjct: 421 ELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPAV 480

Query: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505
           GVGGLLSCWFSCAAKCSSAKLFVRS
Sbjct: 481 GVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of MELO3C004433.jh1.t3 vs. ExPASy TrEMBL
Match: A0A1S3C3I8 (calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 505/532 (94.92%), Postives = 505/532 (94.92%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60
           MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSSSITTQQTGCGG 60

Query: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120
           ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY
Sbjct: 61  ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQY 120

Query: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180
           PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP
Sbjct: 121 PSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRP 180

Query: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240
           SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF
Sbjct: 181 SDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIF 240

Query: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300
           FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS
Sbjct: 241 FVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVS 300

Query: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360
           VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI
Sbjct: 301 VENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSI 360

Query: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420
           VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG
Sbjct: 361 VASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNG 420

Query: 421 ELTKQDFQRAASQV---------------------------CGVSITKNVVDIIFHIFDA 480
           ELTKQDFQRAASQV                           CGVSITKNVVDIIFHIFDA
Sbjct: 421 ELTKQDFQRAASQVSTHQTFQVNKLKINLIHNVGFVFLYKVCGVSITKNVVDIIFHIFDA 480

Query: 481 NEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505
           NEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS
Sbjct: 481 NEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 532

BLAST of MELO3C004433.jh1.t3 vs. ExPASy TrEMBL
Match: A0A0A0LJ54 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_2G009450 PE=4 SV=1)

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 481/511 (94.13%), Postives = 494/511 (96.67%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPL---SSSSSITTQQTG 60
           MFSSTFLVSKSSK ICRVIPSQHLTVRFL+NQLGGSLGSPSTSPL   SSSSSIT+QQTG
Sbjct: 1   MFSSTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLFSSSSSSSITSQQTG 60

Query: 61  CGG-ENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKED 120
           CGG ENRR+FVF+FLIKLISSG+LIGGVGSLLSS SS SNSHLSFADFPKETTWTTVKED
Sbjct: 61  CGGGENRRSFVFDFLIKLISSGILIGGVGSLLSSNSSASNSHLSFADFPKETTWTTVKED 120

Query: 121 QFQYPS-PN-QNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180
           QFQYPS PN QNIN KKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG
Sbjct: 121 QFQYPSSPNHQNINPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEG 180

Query: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLIS 240
           EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERR+SGELCCAPS FFMLFDTNNDGLIS
Sbjct: 181 EIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRMSGELCCAPSTFFMLFDTNNDGLIS 240

Query: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR 300
           FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHR+GRR
Sbjct: 241 FAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHREGRR 300

Query: 301 FGMKVSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVK 360
           FGMKVSVENGGLVEYFFG+DGK SLHHDKFVQFLRQLH+EIL+LEFSHYDFKSQGSIS K
Sbjct: 301 FGMKVSVENGGLVEYFFGQDGKASLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISAK 360

Query: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFS 420
           DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRIT++EFKDFAELRKKLESFSLAIFS
Sbjct: 361 DFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIFS 420

Query: 421 YGKVNGELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480
           YGKVNGELTKQDFQRAAS VCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN
Sbjct: 421 YGKVNGELTKQDFQRAASHVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVN 480

Query: 481 SSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505
           S+QPAVG GGLLSCWF CAAKCS AKLFVRS
Sbjct: 481 STQPAVGAGGLLSCWFGCAAKCSYAKLFVRS 511

BLAST of MELO3C004433.jh1.t3 vs. ExPASy TrEMBL
Match: A0A6J1CPF9 (calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111013100 PE=4 SV=1)

HSP 1 Score: 813 bits (2099), Expect = 4.89e-294
Identity = 425/506 (83.99%), Postives = 455/506 (89.92%), Query Frame = 0

Query: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLG-SPSTSPLSSSSSITTQQTGCG 60
           MFSST LVSKSS SI RV+ SQ LTVR  NNQLGGS   SPS+S L+SS       TG G
Sbjct: 1   MFSSTLLVSKSSASIHRVVTSQRLTVRLFNNQLGGSASTSPSSSLLASS-----HNTGSG 60

Query: 61  GENRRNFVFEFLIKLISSGLLIGGVGSLLSSKSSTSNSHLSFADFPKETTWTTVKEDQFQ 120
            E++R+ +F+FL+K +SSG++  G+GSLL   SSTSNS++SFADF KET WT +KEDQFQ
Sbjct: 61  EESKRSSIFDFLVKSVSSGVVAVGIGSLLCLNSSTSNSNVSFADFQKETKWT-MKEDQFQ 120

Query: 121 YPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMR 180
           YP PNQN  A+KKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFAS+HTPEGEI+M 
Sbjct: 121 YPFPNQN--ARKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASVHTPEGEIFMT 180

Query: 181 PSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYI 240
           P+DLMRAIVPVFPPSESNRVREGFLRGER I GEL C PS+FFMLFD N+DGLISFAEYI
Sbjct: 181 PADLMRAIVPVFPPSESNRVREGFLRGER-IHGELYCVPSEFFMLFDINSDGLISFAEYI 240

Query: 241 FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV 300
           FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVM LMRKQNRQGA HRDGRR GMKV
Sbjct: 241 FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMALMRKQNRQGARHRDGRRLGMKV 300

Query: 301 SVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALS 360
            VENGGLVEYFFG+DGKGSLHHDKFVQFLRQLH+EIL LEFSHYDFKSQGSIS KDFALS
Sbjct: 301 CVENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILRLEFSHYDFKSQGSISAKDFALS 360

Query: 361 IVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVN 420
           +VASADINHIDKLLDRVEALNKEP F+NIRITYEEFKDFAELRKKLESFSLAIFSYGKVN
Sbjct: 361 MVASADINHIDKLLDRVEALNKEPQFRNIRITYEEFKDFAELRKKLESFSLAIFSYGKVN 420

Query: 421 GELTKQDFQRAASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQPA 480
           GELTKQDFQRAAS VCGVSIT+NVVDIIFHIFDAN DGDLSSDEFVRVIQR+EV+SSQ A
Sbjct: 421 GELTKQDFQRAASHVCGVSITRNVVDIIFHIFDANGDGDLSSDEFVRVIQRKEVSSSQAA 480

Query: 481 VGVGGLLSCWFSCAAKCSSAKLFVRS 505
           VG GG LSCWFSC+AKCSSAKLFVRS
Sbjct: 481 VGAGGFLSCWFSCSAKCSSAKLFVRS 497

BLAST of MELO3C004433.jh1.t3 vs. TAIR 10
Match: AT4G32060.1 (calcium-binding EF hand family protein )

HSP 1 Score: 489.2 bits (1258), Expect = 4.0e-138
Identity = 243/370 (65.68%), Postives = 305/370 (82.43%), Query Frame = 0

Query: 133 SKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFP 192
           S F+FGD YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T +GEI M+P+DLMRAIVPVFP
Sbjct: 130 SGFIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFP 189

Query: 193 PSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSF 252
           PSES+ VREG+L GER   GEL C+PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF
Sbjct: 190 PSESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSF 249

Query: 253 SVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV--SVENGGLVEYF 312
           +VAFKMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G+ +  SVE+GGLVEYF
Sbjct: 250 AVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYF 309

Query: 313 FGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHID 372
           FG+DG   L HDKF +F++ L EE+L LEF+HYD+K +GSIS KDFALS+VA+AD +H+ 
Sbjct: 310 FGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLS 369

Query: 373 KLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRA 432
           KLLDRVE+L++ PH +++RI+ +EFK F ELR KL  FSLA+F+YGK NG LT +DF+RA
Sbjct: 370 KLLDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRA 429

Query: 433 ASQVCGVSITKNVVDIIFHIFDANEDGDLSSDEFVRVIQRREVNSSQP-AVGVGGLLSCW 492
           ASQVCG++++ NV++I FH+FD+N+DG+LS DEF+RV+ RRE + +QP A G+    S  
Sbjct: 430 ASQVCGITLSDNVIEIAFHVFDSNQDGNLSVDEFLRVLHRRERDVAQPIAKGLSRYFSDG 489

Query: 493 FSCAAKCSSA 500
           +  +  CSS+
Sbjct: 490 WKGSKNCSSS 498

BLAST of MELO3C004433.jh1.t3 vs. TAIR 10
Match: AT4G32060.2 (calcium-binding EF hand family protein )

HSP 1 Score: 427.9 bits (1099), Expect = 1.1e-119
Identity = 215/319 (67.40%), Postives = 263/319 (82.45%), Query Frame = 0

Query: 133 SKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFP 192
           S F+FGD YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T +GEI M+P+DLMRAIVPVFP
Sbjct: 130 SGFIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFP 189

Query: 193 PSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSF 252
           PSES+ VREG+L GER   GEL C+PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF
Sbjct: 190 PSESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSF 249

Query: 253 SVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKV--SVENGGLVEYF 312
           +VAFKMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G+ +  SVE+GGLVEYF
Sbjct: 250 AVAFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYF 309

Query: 313 FGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHID 372
           FG+DG   L HDKF +F++ L EE+L LEF+HYD+K +GSIS KDFALS+VA+AD +H+ 
Sbjct: 310 FGKDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLS 369

Query: 373 KLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRA 432
           KLLDRVE+L++ PH +++RI+ +EFK F ELR KL  FSLA+F+YGK NG LT +DF+RA
Sbjct: 370 KLLDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRA 429

Query: 433 ASQ--VCGVSITKNVVDII 448
           ASQ    G +  K +V ++
Sbjct: 430 ASQREGRGTANCKRIVSVL 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008456571.10.0100.00PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X2 [Cucumis melo... [more]
KAA0063559.10.099.41calcium uptake protein 1 [Cucumis melo var. makuwa] >TYJ97885.1 calcium uptake p... [more]
XP_008456564.10.094.92PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X1 [Cucumis melo... [more]
XP_004139397.10.094.13calcium uptake protein, mitochondrial [Cucumis sativus] >KGN60757.1 hypothetical... [more]
XP_022143166.11.01e-29383.99calcium uptake protein, mitochondrial-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SZ455.6e-13765.68Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE... [more]
B1H2N31.7e-4530.02Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 P... [more]
A2VEI25.1e-4532.41Calcium uptake protein 1 homolog, mitochondrial OS=Drosophila melanogaster OX=72... [more]
Q95PZ21.9e-4433.15Calcium uptake protein 1 homolog, mitochondrial OS=Caenorhabditis elegans OX=623... [more]
Q4R5181.9e-4432.46Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 ... [more]
Match NameE-valueIdentityDescription
A0A1S3C4U20.0100.00calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 ... [more]
A0A5A7VDG30.099.41Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C3I80.094.92calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A0A0LJ540.094.13Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_2G009450 PE=4 SV=1[more]
A0A6J1CPF94.89e-29483.99calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G32060.14.0e-13865.68calcium-binding EF hand family protein [more]
AT4G32060.21.1e-11967.40calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 217..245
e-value: 0.034
score: 23.3
coord: 251..279
e-value: 2.6E-5
score: 33.7
coord: 443..471
e-value: 1.1
score: 17.4
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 255..277
e-value: 8.9E-7
score: 28.0
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 419..470
e-value: 3.5E-7
score: 29.9
coord: 222..243
e-value: 0.001
score: 18.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 439..474
score: 10.747526
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 247..282
score: 13.118635
IPR002048EF-hand domainCDDcd00051EFhcoord: 220..276
e-value: 3.11983E-11
score: 56.7873
NoneNo IPR availableGENE3D1.10.238.10coord: 156..296
e-value: 9.2E-15
score: 56.6
NoneNo IPR availableGENE3D1.10.238.10coord: 330..479
e-value: 3.1E-12
score: 48.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..58
NoneNo IPR availablePANTHERPTHR12294:SF23CALCIUM UPTAKE PROTEIN, MITOCHONDRIALcoord: 39..502
NoneNo IPR availableCDDcd15900EFh_MICUcoord: 252..471
e-value: 7.97315E-44
score: 150.071
IPR039800Calcium uptake protein 1/2/3PANTHERPTHR12294EF HAND DOMAIN FAMILY A1,A2-RELATEDcoord: 39..502
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 452..464
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 260..272
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 294..399
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 220..469

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C004433.jh1MELO3C004433.jh1gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C004433.jh1.t3.exonMELO3C004433.jh1.t3.exonexon
MELO3C004433.jh1.t3.exonMELO3C004433.jh1.t3.exon_2exon
MELO3C004433.jh1.t3.exonMELO3C004433.jh1.t3.exon_3exon
MELO3C004433.jh1.t3.exonMELO3C004433.jh1.t3.exon_4exon
MELO3C004433.jh1.t3.exonMELO3C004433.jh1.t3.exon_5exon
MELO3C004433.jh1.t3.exonMELO3C004433.jh1.t3.exon_6exon


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C004433.jh1.t3.stop_codonMELO3C004433.jh1.t3.stop_codonstop_codon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C004433.jh1.t3.cdsMELO3C004433.jh1.t3.cdsCDS
MELO3C004433.jh1.t3.cdsMELO3C004433.jh1.t3.cds_2CDS
MELO3C004433.jh1.t3.cdsMELO3C004433.jh1.t3.cds_3CDS
MELO3C004433.jh1.t3.cdsMELO3C004433.jh1.t3.cds_4CDS
MELO3C004433.jh1.t3.cdsMELO3C004433.jh1.t3.cds_5CDS
MELO3C004433.jh1.t3.cdsMELO3C004433.jh1.t3.cds_6CDS


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C004433.jh1.t3.start_codonMELO3C004433.jh1.t3.start_codonstart_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C004433.jh1.t3MELO3C004433.jh1.t3-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006851 mitochondrial calcium ion transmembrane transport
molecular_function GO:0005509 calcium ion binding