MELO3C004092.1 (mRNA) Melon (DHL92) v4

BLAST of MELO3C004092.1 vs. NCBI nr
Match: XP_008452293.1 (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540

Query: 541 LEAGVF 547
           LEAGVF
Sbjct: 541 LEAGVF 546

BLAST of MELO3C004092.1 vs. NCBI nr
Match: KAA0048648.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1062.0 bits (2745), Expect = 1.7e-306
Identity = 539/546 (98.72%), Postives = 539/546 (98.72%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR       YLNNEEATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR-------YLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 539

Query: 541 LEAGVF 547
           LEAGVF
Sbjct: 541 LEAGVF 539

BLAST of MELO3C004092.1 vs. NCBI nr
Match: TYK10849.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.2e-302
Identity = 534/546 (97.80%), Postives = 534/546 (97.80%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHPQSSLSLPRNHP            GYLNNEEATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHP------------GYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 534

Query: 541 LEAGVF 547
           LEAGVF
Sbjct: 541 LEAGVF 534

BLAST of MELO3C004092.1 vs. NCBI nr
Match: XP_004149075.1 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KGN61091.1 hypothetical protein Csa_021253 [Cucumis sativus])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.4e-300
Identity = 522/547 (95.43%), Postives = 540/547 (98.72%), Query Frame = 0

Query: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIRGPQIMKGYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of MELO3C004092.1 vs. NCBI nr
Match: XP_038890953.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 980.7 bits (2534), Expect = 5.1e-282
Identity = 492/546 (90.11%), Postives = 514/546 (94.14%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MDH   NGQDFIF+SKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLING TG +HTYA VD
Sbjct: 1   MDHGLMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGATGEVHTYAAVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARR AAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI KQ
Sbjct: 61  LAARRVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           A  AR KVIITQ EFVEKVW +AVE+GVKILCTD    S   GCLRFSEVMEADENDIPA
Sbjct: 121 AAGARPKVIITQAEFVEKVWGYAVEHGVKILCTD----SPPVGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+ S DVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLYSNDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILIL+KYDMSSLL LIQ +KATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILKKYDMSSLLPLIQTYKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
            Y+LSSIRIVMSGAAPMGKDLEDTVKARLPNA LGQGYGMTEAGPVLSMCL FAKEPF V
Sbjct: 301 RYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEV 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKII+P++S+SLPRNHPGEICIRG QIMKGY+NN EATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIINPETSVSLPRNHPGEICIRGSQIMKGYINNREATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDD+DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNI+SAAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDNDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNISSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIPVAFVVRSKGSKISE+D+K YISDQVIYYK+IRKVFFVDSIP+APSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISEDDLKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQ 540

Query: 541 LEAGVF 547
           LEAG F
Sbjct: 541 LEAGAF 542

BLAST of MELO3C004092.1 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 7.0e-226
Identity = 383/541 (70.79%), Postives = 458/541 (84.66%), Query Frame = 0

Query: 2   DHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVDL 61
           DH+    ++FIF+SKLPDIYIPNHLPLH+YCFE+++QF+ RPCLING TG I TYA+VDL
Sbjct: 8   DHQ----EEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDL 67

Query: 62  AARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQA 121
            +R+ AAGL K+GI QGDVIML+LQNSPEFV+AFL AS+ GAI TTANP Y P E+ KQA
Sbjct: 68  TSRKVAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQA 127

Query: 122 VAARAKVIITQGEFVEKVWEF-AVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 181
            A++ K++IT   +++KV EF   E+GVK++C D+        CL FSE+ +ADE +IPA
Sbjct: 128 AASKTKLVITLAGYIDKVKEFTGGESGVKVMCVDAPPPESE--CLHFSELTQADETEIPA 187

Query: 182 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 241
           VKI+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDG+NPN+  +  DVI+CVLPL
Sbjct: 188 VKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPL 247

Query: 242 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 301
           FHIYSLNS+LLCGLRVGAAILI+QK+++S LL LI+  K TIAPFVPPIVL  AK PD+H
Sbjct: 248 FHIYSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLH 307

Query: 302 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 361
            Y+LSSIR VMSG APMGK+LED VK +LP+A LGQGYGMTEAGPVLSMCL FAKEPF +
Sbjct: 308 RYDLSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPI 367

Query: 362 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 421
           KSGACGTVVRNAEMKI+ P +  SLPRN  GEICIRG QIMKGY+N+ EAT+ TID+ GW
Sbjct: 368 KSGACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGW 427

Query: 422 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 481
           LHTGD+GFID+DDE+FIVDRLKELIKYKG+QVAPAELE++L S+PNIT AAV+PM+DEAA
Sbjct: 428 LHTGDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAA 487

Query: 482 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 541
           GE+PVAFVVRS GSKI+E DIK YIS QV++YKRI K FF++ IP  PSGKILRK+L+A+
Sbjct: 488 GEVPVAFVVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAK 542

BLAST of MELO3C004092.1 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 3.9e-224
Identity = 374/545 (68.62%), Postives = 464/545 (85.14%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MD E +   D IF+SKLPDIYIP HLPLH+YCFE+L++F  RPCLI+G   RI+TYAEV+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           L +R+ A GL+K+GI Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VKQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           A A+ AK++ITQ  F  KV ++A+EN +K++C DS+      GC+ FSE++++DE++IP 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSA----PEGCVHFSELIQSDEHEIPD 182

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+S DV++CVLPL
Sbjct: 183 VKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPL 242

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +H
Sbjct: 243 FHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVH 302

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NY+LSS+R VMSGAAP+GK+LED V+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPF++
Sbjct: 303 NYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDI 362

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKI+ P +  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+GW
Sbjct: 363 KSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGW 422

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE A
Sbjct: 423 LHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQA 482

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GE+PVAFVVRS GS I+E+++K++IS QVI+YKRI++VFFV+++P +PSGKILRK L+A+
Sbjct: 483 GEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRAR 542

Query: 541 LEAGV 546
           L AG+
Sbjct: 543 LAAGI 543

BLAST of MELO3C004092.1 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 4.3e-223
Identity = 372/542 (68.63%), Postives = 463/542 (85.42%), Query Frame = 0

Query: 4   EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVDLAA 63
           E +   D IF+SKLPDIYIP HLPLH+YCFE++++F  RPCLING   +I+TYAEV+L  
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVA 123
           R+ A GL+K+GI Q D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VKQA A
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPAVKI 183
           + AK+IITQ  FV KV ++A EN VK++C DS+      GCL FSE+ ++DE++IP VKI
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSA----PEGCLHFSELTQSDEHEIPEVKI 187

Query: 184 NSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPLFHI 243
             +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+S+DV++CVLPLFHI
Sbjct: 188 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 247

Query: 244 YSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIHNYN 303
           YSLNS+LLCGLRVGAAILI+QK+D++  L LIQ +K +I PFVPPIVL  AK+P + +Y+
Sbjct: 248 YSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYD 307

Query: 304 LSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNVKSG 363
           LSS+R VMSGAAP+GK+LED V+ + PNA LGQGYGMTEAGPVL+MCL FAKEPF++KSG
Sbjct: 308 LSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 367

Query: 364 ACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGWLHT 423
           ACGTVVRNAEMKI+ P +  SLPRN PGEICIRG QIMKGYLN+ EAT +TIDK+GWLHT
Sbjct: 368 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHT 427

Query: 424 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEI 483
           GD+GFID+DDE+FIVDRLKELIKYKG+QVAPAE+EALL ++PNI+ AAV+PM+DE AGE+
Sbjct: 428 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEV 487

Query: 484 PVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQLEA 543
           PVAFVVRS GS I+E+++K++IS QVI+YKR+++VFFV+++P +PSGKILRK L+A+L A
Sbjct: 488 PVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAA 545

Query: 544 GV 546
           GV
Sbjct: 548 GV 545

BLAST of MELO3C004092.1 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 7.3e-223
Identity = 373/545 (68.44%), Postives = 462/545 (84.77%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MD E +   D IF+SKLPDIYIP HLPLH+YCFE+L++F  RPCLI+G   RI+TYAEV+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           L +R+ A GL+K+GI Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VKQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           A A+ AK++ITQ  F  KV ++A+EN +K++C D    S   GC+ FSE++++DE++IP 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVD----SVPEGCVHFSELIQSDEHEIPD 182

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+S DV++CVLPL
Sbjct: 183 VKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPL 242

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P + 
Sbjct: 243 FHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVD 302

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NY+LSS+R VMSGAAP+GK+LED V+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPF++
Sbjct: 303 NYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDI 362

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKI+ P +  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+GW
Sbjct: 363 KSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGW 422

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE A
Sbjct: 423 LHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQA 482

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GE+PVAFVVRS GS I+E+++K++IS QVI+YKRI++VFFV+++P +PSGKILRK L+A+
Sbjct: 483 GEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRAR 542

Query: 541 LEAGV 546
           L AG+
Sbjct: 543 LAAGI 543

BLAST of MELO3C004092.1 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 2.8e-222
Identity = 371/541 (68.58%), Postives = 461/541 (85.21%), Query Frame = 0

Query: 5   QRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVDLAAR 64
           + N  D IF+SKLPDIYIP HLPLH+YCFE++++F  RPCLING    I+TYA+V+L +R
Sbjct: 6   ETNQGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVELTSR 65

Query: 65  RFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAA 124
           + AAGL+K+GI Q D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VKQA A+
Sbjct: 66  KVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKAS 125

Query: 125 RAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPAVKIN 184
            AK+IITQ  FV KV ++A +N + ++C DS+      GC+ FSE+ +ADE+DIP VKI 
Sbjct: 126 NAKLIITQACFVNKVKDYAFDNNLNVICIDSA----PEGCIHFSELTQADEHDIPDVKIQ 185

Query: 185 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPLFHIY 244
           S+DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+S+DV++CVLPLFHIY
Sbjct: 186 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 245

Query: 245 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIHNYNL 304
           SLNSVLLCGLRVGAAILI+QK+D+     LI+ +K TI PFVPPIVL  AK+P + NY+L
Sbjct: 246 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 305

Query: 305 SSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNVKSGA 364
           SS+R VMSGAAP+GK+LED V+ + PNA LGQGYGMTEAGPVL+MCL FAKEPF++KSGA
Sbjct: 306 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 365

Query: 365 CGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGWLHTG 424
           CGTVVRNAEMKI+ P +  SLPRN PGEICIRG QIMKGYLN+  AT +TIDK+GWLHTG
Sbjct: 366 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 425

Query: 425 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIP 484
           D+G+ID+DDE+FIVDRLKELIKYKG+QVAPAELEALL ++PNI+ AAV+PM+DE AGE+P
Sbjct: 426 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 485

Query: 485 VAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQLEAG 544
           VAFVVRS GS I+E+++K+++S QVI+YKRI++VFFV+++P +PSGKILRK L+A+L AG
Sbjct: 486 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 545 V 546
           V
Sbjct: 546 V 542

BLAST of MELO3C004092.1 vs. ExPASy TrEMBL
Match: A0A1S3BSW8 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540

Query: 541 LEAGVF 547
           LEAGVF
Sbjct: 541 LEAGVF 546

BLAST of MELO3C004092.1 vs. ExPASy TrEMBL
Match: A0A5A7U0C7 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1468G00210 PE=4 SV=1)

HSP 1 Score: 1062.0 bits (2745), Expect = 8.4e-307
Identity = 539/546 (98.72%), Postives = 539/546 (98.72%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR       YLNNEEATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR-------YLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 539

Query: 541 LEAGVF 547
           LEAGVF
Sbjct: 541 LEAGVF 539

BLAST of MELO3C004092.1 vs. ExPASy TrEMBL
Match: A0A5D3CKT4 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00190 PE=4 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 5.7e-303
Identity = 534/546 (97.80%), Postives = 534/546 (97.80%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
           NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHPQSSLSLPRNHP            GYLNNEEATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHP------------GYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 534

Query: 541 LEAGVF 547
           LEAGVF
Sbjct: 541 LEAGVF 534

BLAST of MELO3C004092.1 vs. ExPASy TrEMBL
Match: A0A0A0LM95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G048330 PE=4 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.6e-300
Identity = 522/547 (95.43%), Postives = 540/547 (98.72%), Query Frame = 0

Query: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIRGPQIMKGYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of MELO3C004092.1 vs. ExPASy TrEMBL
Match: A0A6J1EP35 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436262 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 480/546 (87.91%), Postives = 509/546 (93.22%), Query Frame = 0

Query: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60
           MD +  N QDFIF+SKLPDIYIPNHLPLHTYCFEHL+QF HRPCLIN  TG+IHTYA VD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120
           LAARR A GLSKIG+GQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180
           A  AR KVIITQ EFVEKV EFAVENGVKI+CTD    S   GCLRFSE++EADEN+IPA
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++NS DV+IC+LPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360
            Y+LSSIRIVMSGAAPMGK LEDTVKARLPNA LGQGYGMTEAGPVLSMCLGFAKEPF  
Sbjct: 301 RYDLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEF 360

Query: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHP++ +SLPRNHPGEICIRG QIMKGYLNN+EATEKTIDK+GW
Sbjct: 361 KSGACGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540
           GEIP AFVVRSKGSKISE+DIK YISDQVIYYK+IRKVFFVDSIP+APSGKILRKVLKAQ
Sbjct: 481 GEIPAAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQ 540

Query: 541 LEAGVF 547
           LE G F
Sbjct: 541 LENGAF 542

BLAST of MELO3C004092.1 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 711.4 bits (1835), Expect = 5.4e-205
Identity = 350/543 (64.46%), Postives = 436/543 (80.29%), Query Frame = 0

Query: 7   NGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVDLAARRF 66
           N  D IF+SKLPDIYIPNHL LH Y F+++++F  +PCLING TG ++TY++V + +R+ 
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  AAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAARA 126
           AA   K+G+ Q DV+ML+L N PEFV +FL ASF GA AT ANP + P EI KQA A+  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQGEFVEKVWEFAVENGVKILC-TDSSSASESAGCLRFSEVMEAD---ENDIPAVK 186
           K+IIT+  +V+K+     ++GV I+C  D+ S     GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +S DVI+CVLP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIHNY 306
           IY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +   Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 NLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNVKS 366
           +LSSIR+V SGAAP+GK+LED V A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF VKS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGWLH 426
           GACGTVVRNAEMKI+ P +  SL RN PGEICIRG QIMKGYLNN  AT +TIDKDGWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGE 486
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+IT  AV+ M++EAAGE
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 498

Query: 487 IPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQLE 546
           +PVAFVV+SK S++SE+D+K ++S QV++YKRI KVFF +SIP APSGKILRK L+A+L 
Sbjct: 499 VPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of MELO3C004092.1 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 705.3 bits (1819), Expect = 3.9e-203
Identity = 350/544 (64.34%), Postives = 435/544 (79.96%), Query Frame = 0

Query: 4   EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVDLAA 63
           +++   D IF+S+LPDIYIPNHLPLH Y FE++++F  +PCLING TG ++TYA+V + +
Sbjct: 14  QKQCSNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTS 73

Query: 64  RRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVA 123
           R+ AAGL  +G+ Q DV+M++L NSPE V  FL ASF GAI T+ANP + P EI KQA A
Sbjct: 74  RKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKA 133

Query: 124 ARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPAV-- 183
           + AK+I+TQ  +V+K+      +GV I+ TDS +  E+  CLRFSE+ +++E  + ++  
Sbjct: 134 SAAKLIVTQSRYVDKIKNLQ-NDGVLIVTTDSDAIPEN--CLRFSELTQSEEPRVDSIPE 193

Query: 184 KINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPLF 243
           KI+  DVVALPFSSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  N  DVI+CVLP+F
Sbjct: 194 KISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMF 253

Query: 244 HIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIHN 303
           HIY+LNS++LC LRVGA ILI+ K++++ LL  IQ  K T+A  VPPIVL  AK+P+   
Sbjct: 254 HIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEK 313

Query: 304 YNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNVK 363
           Y+LSS+R+V SGAAP+GK+LED + A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF VK
Sbjct: 314 YDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 373

Query: 364 SGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGWL 423
           SGACGTVVRNAEMKI+ P +  SLPRN PGEICIRG QIMKGYLN+  AT  TIDKDGWL
Sbjct: 374 SGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWL 433

Query: 424 HTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAG 483
           HTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P I   AV+ M++E AG
Sbjct: 434 HTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAG 493

Query: 484 EIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQL 543
           E+PVAFVVRSK S ISE++IK ++S QV++YKRI KVFF DSIP APSGKILRK L+A+L
Sbjct: 494 EVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 553

Query: 544 EAGV 546
             G+
Sbjct: 554 ANGL 554

BLAST of MELO3C004092.1 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 665.2 bits (1715), Expect = 4.4e-191
Identity = 327/532 (61.47%), Postives = 419/532 (78.76%), Query Frame = 0

Query: 12  IFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVDLAARRFAAGLS 71
           IF+SKLPDI IPNHLPLHTYCFE L+    +PCLI G+TG+ +TY E  L  RR A+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAARAKVIIT 131
           K+GI +GDVIM++LQNS EFVF+F+GAS  GA++TTANP Y   E+ KQ  ++ AK+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADEND--IPAVKINSNDVV 191
             ++V+K+        + ++ TD  +      CL FS ++  DE +     V I  +D  
Sbjct: 154 HSQYVDKLKNLG--ENLTLITTDEPTPE---NCLPFSTLITDDETNPFQETVDIGGDDAA 213

Query: 192 ALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPLFHIYSLNSV 251
           ALPFSSGTTG+PKGV+LTHKSL+TSVAQQVDG+NPN+ + S DVI+CVLPLFHIYSLNSV
Sbjct: 214 ALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSV 273

Query: 252 LLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIHNYNLSSIRI 311
           LL  LR GA +L++ K+++ +LL LIQ H+ TIA  VPP+V+  AKNP +++Y+LSS+R 
Sbjct: 274 LLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF 333

Query: 312 VMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNVKSGACGTVV 371
           V+SGAAP+GK+L+D+++ RLP AILGQGYGMTEAGPVLSM LGFAKEP   KSG+CGTVV
Sbjct: 334 VLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVV 393

Query: 372 RNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGWLHTGDLGFI 431
           RNAE+K++H ++ LSL  N PGEICIRG QIMK YLN+ EAT  TID++GWLHTGD+G++
Sbjct: 394 RNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYV 453

Query: 432 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVV 491
           D+DDEIFIVDRLKE+IK+KG+QV PAELE+LL ++ +I  AAV+P  DE AGE+PVAFVV
Sbjct: 454 DEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVV 513

Query: 492 RSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQL 542
           RS G+ I+E D+K Y++ QV++YKR+ KVFFV SIP +PSGKILRK LKA+L
Sbjct: 514 RSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of MELO3C004092.1 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 663.7 bits (1711), Expect = 1.3e-190
Identity = 326/507 (64.30%), Postives = 406/507 (80.08%), Query Frame = 0

Query: 7   NGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVDLAARRF 66
           N  D IF+SKLPDIYIPNHL LH Y F+++++F  +PCLING TG ++TY++V + +R+ 
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  AAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAARA 126
           AA   K+G+ Q DV+ML+L N PEFV +FL ASF GA AT ANP + P EI KQA A+  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQGEFVEKVWEFAVENGVKILC-TDSSSASESAGCLRFSEVMEAD---ENDIPAVK 186
           K+IIT+  +V+K+     ++GV I+C  D+ S     GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +S DVI+CVLP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIHNY 306
           IY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +   Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 NLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNVKS 366
           +LSSIR+V SGAAP+GK+LED V A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF VKS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGWLH 426
           GACGTVVRNAEMKI+ P +  SL RN PGEICIRG QIMKGYLNN  AT +TIDKDGWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGE 486
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+IT  AV+ M++EAAGE
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 498

Query: 487 IPVAFVVRSKGSKISENDIKNYISDQV 510
           +PVAFVV+SK S++SE+D+K ++S QV
Sbjct: 499 VPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of MELO3C004092.1 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 645.2 bits (1663), Expect = 4.7e-185
Identity = 313/550 (56.91%), Postives = 420/550 (76.36%), Query Frame = 0

Query: 2   DHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLA----QFQHRPCLINGTTGRIHTYA 61
           D E+    DFIF+SKLPDI+IPNHLPL  Y F+  +          C+I+G TGRI TYA
Sbjct: 18  DEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYA 77

Query: 62  EVDLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEI 121
           +V    RR AAG+ ++GI  GDV+ML+L NSPEF  +FL  ++ GA++TTANP Y   EI
Sbjct: 78  DVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEI 137

Query: 122 VKQAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSS-----ASESAGCLRFSEVME 181
            KQA A+ AK+IIT+   V+K+      +GV I+C D        +S   GC+ F+E+ +
Sbjct: 138 AKQAKASAAKMIITKKCLVDKLTNLK-NDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQ 197

Query: 182 ADENDIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKD 241
           ADE ++   KI+  D VA+P+SSGTTG+PKGVM+THK LVTS+AQ+VDGENPN+N  + D
Sbjct: 198 ADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTAND 257

Query: 242 VIICVLPLFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLD 301
           VI+C LP+FHIY+L++++L  +R GAA+LI+ +++++ ++ LIQ +K T+ P  PP+VL 
Sbjct: 258 VILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLA 317

Query: 302 FAKNPDIHNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLG 361
           F K+P+   Y+LSS+RI++SGAA + K+LED V+ + PNAI GQGYGMTE+G V +  L 
Sbjct: 318 FIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLA 377

Query: 362 FAKEPFNVKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATE 421
           FAK PF  KSGACGTV+RNAEMK++  ++ +SLPRN  GEIC+RG Q+MKGYLN+ EAT 
Sbjct: 378 FAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATA 437

Query: 422 KTIDKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAV 481
           +TIDKDGWLHTGD+GF+DDDDEIFIVDRLKELIK+KGYQVAPAELEALL S+P+I  AAV
Sbjct: 438 RTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAV 497

Query: 482 IPMEDEAAGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKI 541
           + M+DE A E+PVAFV RS+GS+++E+D+K+Y++ QV++YKRI+ VFF++ IP A SGKI
Sbjct: 498 VAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKI 557

Query: 542 LRKVLKAQLE 543
           LRK L+A+LE
Sbjct: 558 LRKDLRAKLE 565