MELO3C003651.jh1.t1 (mRNA) Melon (Harukei-3) v1.41

Overview
NameMELO3C003651.jh1.t1
TypemRNA
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionAspartate--tRNA ligase
Locationchr04: 2859101 .. 2862972 (-)
Sequence length2302
RNA-Seq ExpressionMELO3C003651.jh1.t1
SyntenyMELO3C003651.jh1.t1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTACACTTCTTTCCCTTTCAACGGTCATCTCCCTCACTCTCGGGCCGCCGTCGCCAACAGTCATCTCCGGATGAATTTCCGGCGCCGGCCCGGATTGGTTCCTCTTATCCTAAACCCTTTACCCAACCGAGCGGTTCGGTTCATATATGACACGAACCGAATGAGCCGAGCCCCATGTTTAGACCATTGTATTCGTCCAGCCCATCTTATCAGTAGCCGCCTTCACTGACCGAGAGACGCCGTTTTCTCTCTTCCATTTTCCTTCAAAACCCTCAATCTCCCTTCTCATTTCCATATACTCCAACCATGTCCGCTTCATCCCAAACTCCGATCGACGAGCCGCTTTCTGCCGGCGATGAATCTTCCAAAGCCGTCAGCAAGAAAGCCGCCAAGAAGGAGGCTGCAAAGCTAGAAAAGCTCCGCCGGCGCCAAGAAGCAGCTGCAGAATCTGCTATTTCGTCTCTCAACGTCGAGGACGATCCGCTTTCCGCTAACTATGGCGATGTACCACTCTCCGATCTCCAATCAAAGGAGGTTAAGAGTATTGAAAACTGGACTCAGGTTGGCTCTTTGACGCCGGAGCTTAAAGACAAGTATGTGATTCTACGGGGTCGTATTCAGACGATTCGTGCTGTTGGGAAGAAGATGGCGTTTTTGGTGGTGAGAGAAAAGGGATTCACCGTGCAATGCGTTTTGTCGGAGCAGCCGGAGCTTGTTAGTCGCCAGATGGTGAAGTATGTTGATGGATTGAGCCGTGAGTCCATCGTTGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTTCCCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTTTGCCTATAAATATCGAAGATGCTGCCCGTAGTGAAGCTGAAATTGAGAAGGCCTTGCAGGTATACTTTGTAATCGCTTTCTTTTAATGTGATCTTCGTGATTGTCTAATGTCTGTTGTGAGTTCTATACATGCACACACGCACACGCACACGCACACATATAATCTTGGTTTCACTCTGTAGTGCTTTTTAACCAAAAGCAAAGAGACTTGTGGACCTCCTGTTTAATGTTAATCTCATTTGTTGTCCGCAGGCTGGTGAGCAACTGGTACGTGTTAATCAGGATACGCGTTTGAACTACAGAGTCCTCGATATGCGGACTCCAGCTAATCAAGGGATTTTTCGCATCCAATGTCAAGTTTCAACGGTGAGTTCTCTTTGTTATTTTATATTTTTAAAGGTTCTTTGGTCTAAATCAATGGTTATATTACATGAAATTGGAGTATTCGGGATTTATTCAAAGTTGATTCTGGTAGATATAGCTATCATTGTCGTCCTGCTTGGTTAGGTTCTTGTTGCCTTGTTCATTGGCCATCTGTAGCTTGTAGGAAGAGTCGATTTGTTTTGTGGGGTTGGAGTTCTGGGGAGGATCTTTGGGCTTTGATTATATAATTTATATTAAAATCCTCTTTCTGGATGTATCTTAATAGTTTTGGTTATTATCAGCTTAGCCTTATTACTCTTTTGGATTGGGACCCCTTGTGTAATTGGATGTGAGGGTTTTTTTGTATCTGTGATTGTTTTTTTTTTTTTTTAATTGTATGCCCTTGTTTATTCTTTCAATATTTTCTATGAAAAGATTTGTTGTCTTTAAAAATCCTTTGGCTGCCATTAATCACTCCTTTTTCTCTCTTTGGTGTGAAAAGGAATTGGAATTCTTTCTTGTAACTCACTGTCGTTGTTGACAATAAGATTGTAAACAAGGAATTTGTAACGTTTAGCTATTTCCCCATGTAGTTTCACTTCTCATGTTAAAAGAAGCACGGTCGTTGATTATATTTTCATCATGCAGATGTTTAGACAATTTCTGTTGGATGAAGACTTTGTTGAAATCCACACTCCAAAATTGATTGGAGGTTCTAGTGAAGGCGGTGCTTCTGTTTTTAGGTTGGATTACAAAGGCCAACCCGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCGATCTGTGGTGACTTTGGGCGTGTATTTGAGATTGGTCCTGTTTTTAGAGCAGAAGACTCGTACACACACAGACATTTATGTGAATTTACGGGACTTGATGTTGAAATGGAGATCAAGACTCACTATTCTGAGGTAATGCTCTAAGTTGGCTTTTCATTTGTCTTGGGCATAGTTATTTGATATTGGATCCGACTTTTTTTCTTCTGTTTTAGTGCAGGTCATGGACATTGTGGACCGTTTGTTTGTTGCAATGTTTGATAGTTTGAATGAAAGATGTAAGAAAGCGCTTGAAGCAGTTGATAAACAGTATCCCTTTGAACCTTTGAAGGTGAACTTATTTATTGATTGCTTACTTTGTAAATATTTCATACGCTTTGAAGGTGAACTTATTTTTGGTTATGGATTTGTGCCAATAGAAGAGATGTACAAGTACAATGTGGATATATGACTTAGTATGAATAGTTGAATGATTTTCTCTCATTTCCATCTTTGATTCAATTTCATGAATATGCAGTATTTGCGAAAAACTCTACGACTTACATTTGAGGAAGGAATTCAGATGTTGAAGGTTAGTACTTAGTGGTCCAATCTCTTTTTCCCTCGCGAATCATCAAAAGATTCCAAGACCCAAATATCTTGACATTTTTTCCTTCCTTTGTTATTATGATTATCATCATAGGATGCTGGCGTTGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGGCAACTCGTCTTAGAGAAGTAATTAGGGGTTCTTGCTTATTTAGTTATGAACTTGTTGATTTTGTTCAATTATTATATCTTAAGATTCCTGATGAAATTTTCCTAACTATAGATATGGCACGGAATTCTACATTCTTCACCGCTATCCTTTGGCTGTTAGGCCATTCTACACTATGCCTTGCTACGATAACCCAACTTACAGTAACTCATTTGACGTCTTTATTCGAGGTATGCATTGCATATTACATCACTATAATTGTCTTACCAGTGCTCATTCTTCCTTAAAATTCGGGTTCATATTCATTCTACAGAACAAAATTTGACAATACATCCTTCATGGTAAGTAACTTAGAACATTGTTGTCCATGCAGGGGAAGAAATCATTTCTGGGGCCCAGCGTGTGCATGTGCCAGAATTTCTAGCTGAACGCGCACAAGCATGTGGCATTGACGTAAAAACAATAGAAACATATATTGATTCATTCAGGTAGTTTGCTCTTTGTTTTCTCCTTTACTCAAAGTTGGAATTTAACATGAGTAAGCTAGATCCAATTAGGTTCATCTCCGACATTTTTTATGACATGAATGCTTTCGTTGCAGATATGGTGCCCCTCCGCATGGTGGATTTGGAGTAGGTTTGGAGCGCGTGGTGATGCTGTTTTGCGGTTTGAATAACATTCGCAAAACATCGTTGTTCCCTCGTGATCCACAGAGGATTGCTCCTTAGGCATTTCACAAGTAACACTGCAATTCCAGTTTTTGAAATATACATTTTGTTGCCTTCTTTCTTTCTTTCTCCCTTCATAGGAAAGGAAAACAAACAAATTTCTGATCGACTGATTATTATGGTTTACTGAATAACAATTGTTTGTTTTTCATCGTTGCGAATTCCAAGTTTGTTTTGAACAACTGTTACTTCATGTAGATTTTTATTATAGTTTTTCTTTGTCTGAAATTGATAATTTGACAACTTCTCCGAGTCAAACCTTTGTTCTTTTTGGGTTAAAAATCGAAGAACTCTTCAATAATGATGATATTTTTCTCGTGATGCTTTGATTATGGAGATGAGACAATTTAGAAGAG

mRNA sequence

CTCTACACTTCTTTCCCTTTCAACGGTCATCTCCCTCACTCTCGGGCCGCCGTCGCCAACAGTCATCTCCGGATGAATTTCCGGCGCCGGCCCGGATTGGTTCCTCTTATCCTAAACCCTTTACCCAACCGAGCGGTTCGGTTCATATATGACACGAACCGAATGAGCCGAGCCCCATGTTTAGACCATTGTATTCGTCCAGCCCATCTTATCAGTAGCCGCCTTCACTGACCGAGAGACGCCGTTTTCTCTCTTCCATTTTCCTTCAAAACCCTCAATCTCCCTTCTCATTTCCATATACTCCAACCATGTCCGCTTCATCCCAAACTCCGATCGACGAGCCGCTTTCTGCCGGCGATGAATCTTCCAAAGCCGTCAGCAAGAAAGCCGCCAAGAAGGAGGCTGCAAAGCTAGAAAAGCTCCGCCGGCGCCAAGAAGCAGCTGCAGAATCTGCTATTTCGTCTCTCAACGTCGAGGACGATCCGCTTTCCGCTAACTATGGCGATGTACCACTCTCCGATCTCCAATCAAAGGAGGTTAAGAGTATTGAAAACTGGACTCAGGTTGGCTCTTTGACGCCGGAGCTTAAAGACAAGTATGTGATTCTACGGGGTCGTATTCAGACGATTCGTGCTGTTGGGAAGAAGATGGCGTTTTTGGTGGTGAGAGAAAAGGGATTCACCGTGCAATGCGTTTTGTCGGAGCAGCCGGAGCTTGTTAGTCGCCAGATGGTGAAGTATGTTGATGGATTGAGCCGTGAGTCCATCGTTGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTTCCCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTTTGCCTATAAATATCGAAGATGCTGCCCGTAGTGAAGCTGAAATTGAGAAGGCCTTGCAGGCTGGTGAGCAACTGGTACGTGTTAATCAGGATACGCGTTTGAACTACAGAGTCCTCGATATGCGGACTCCAGCTAATCAAGGGATTTTTCGCATCCAATGTCAAGTTTCAACGATGTTTAGACAATTTCTGTTGGATGAAGACTTTGTTGAAATCCACACTCCAAAATTGATTGGAGGTTCTAGTGAAGGCGGTGCTTCTGTTTTTAGGTTGGATTACAAAGGCCAACCCGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCGATCTGTGGTGACTTTGGGCGTGTATTTGAGATTGGTCCTGTTTTTAGAGCAGAAGACTCGTACACACACAGACATTTATGTGAATTTACGGGACTTGATGTTGAAATGGAGATCAAGACTCACTATTCTGAGGTCATGGACATTGTGGACCGTTTGTTTGTTGCAATGTTTGATAGTTTGAATGAAAGATGTAAGAAAGCGCTTGAAGCAGTTGATAAACAGTATCCCTTTGAACCTTTGAAGTATTTGCGAAAAACTCTACGACTTACATTTGAGGAAGGAATTCAGATGTTGAAGGATGCTGGCGTTGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGGCAACTCGTCTTAGAGAAATATGGCACGGAATTCTACATTCTTCACCGCTATCCTTTGGCTGTTAGGCCATTCTACACTATGCCTTGCTACGATAACCCAACTTACAGTAACTCATTTGACGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCCCAGCGTGTGCATGTGCCAGAATTTCTAGCTGAACGCGCACAAGCATGTGGCATTGACGTAAAAACAATAGAAACATATATTGATTCATTCAGATATGGTGCCCCTCCGCATGGTGGATTTGGAGTAGGTTTGGAGCGCGTGGTGATGCTGTTTTGCGGTTTGAATAACATTCGCAAAACATCGTTGTTCCCTCGTGATCCACAGAGGATTGCTCCTTAGGCATTTCACAAGTAACACTGCAATTCCAGTTTTTGAAATATACATTTTGTTGCCTTCTTTCTTTCTTTCTCCCTTCATAGGAAAGGAAAACAAACAAATTTCTGATCGACTGATTATTATGGTTTACTGAATAACAATTGTTTGTTTTTCATCGTTGCGAATTCCAAGTTTGTTTTGAACAACTGTTACTTCATGTAGATTTTTATTATAGTTTTTCTTTGTCTGAAATTGATAATTTGACAACTTCTCCGAGTCAAACCTTTGTTCTTTTTGGGTTAAAAATCGAAGAACTCTTCAATAATGATGATATTTTTCTCGTGATGCTTTGATTATGGAGATGAGACAATTTAGAAGAG

Coding sequence (CDS)

ATGTCCGCTTCATCCCAAACTCCGATCGACGAGCCGCTTTCTGCCGGCGATGAATCTTCCAAAGCCGTCAGCAAGAAAGCCGCCAAGAAGGAGGCTGCAAAGCTAGAAAAGCTCCGCCGGCGCCAAGAAGCAGCTGCAGAATCTGCTATTTCGTCTCTCAACGTCGAGGACGATCCGCTTTCCGCTAACTATGGCGATGTACCACTCTCCGATCTCCAATCAAAGGAGGTTAAGAGTATTGAAAACTGGACTCAGGTTGGCTCTTTGACGCCGGAGCTTAAAGACAAGTATGTGATTCTACGGGGTCGTATTCAGACGATTCGTGCTGTTGGGAAGAAGATGGCGTTTTTGGTGGTGAGAGAAAAGGGATTCACCGTGCAATGCGTTTTGTCGGAGCAGCCGGAGCTTGTTAGTCGCCAGATGGTGAAGTATGTTGATGGATTGAGCCGTGAGTCCATCGTTGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTTCCCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTTTGCCTATAAATATCGAAGATGCTGCCCGTAGTGAAGCTGAAATTGAGAAGGCCTTGCAGGCTGGTGAGCAACTGGTACGTGTTAATCAGGATACGCGTTTGAACTACAGAGTCCTCGATATGCGGACTCCAGCTAATCAAGGGATTTTTCGCATCCAATGTCAAGTTTCAACGATGTTTAGACAATTTCTGTTGGATGAAGACTTTGTTGAAATCCACACTCCAAAATTGATTGGAGGTTCTAGTGAAGGCGGTGCTTCTGTTTTTAGGTTGGATTACAAAGGCCAACCCGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCGATCTGTGGTGACTTTGGGCGTGTATTTGAGATTGGTCCTGTTTTTAGAGCAGAAGACTCGTACACACACAGACATTTATGTGAATTTACGGGACTTGATGTTGAAATGGAGATCAAGACTCACTATTCTGAGGTCATGGACATTGTGGACCGTTTGTTTGTTGCAATGTTTGATAGTTTGAATGAAAGATGTAAGAAAGCGCTTGAAGCAGTTGATAAACAGTATCCCTTTGAACCTTTGAAGTATTTGCGAAAAACTCTACGACTTACATTTGAGGAAGGAATTCAGATGTTGAAGGATGCTGGCGTTGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGGCAACTCGTCTTAGAGAAATATGGCACGGAATTCTACATTCTTCACCGCTATCCTTTGGCTGTTAGGCCATTCTACACTATGCCTTGCTACGATAACCCAACTTACAGTAACTCATTTGACGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCCCAGCGTGTGCATGTGCCAGAATTTCTAGCTGAACGCGCACAAGCATGTGGCATTGACGTAAAAACAATAGAAACATATATTGATTCATTCAGATATGGTGCCCCTCCGCATGGTGGATTTGGAGTAGGTTTGGAGCGCGTGGTGATGCTGTTTTGCGGTTTGAATAACATTCGCAAAACATCGTTGTTCCCTCGTGATCCACAGAGGATTGCTCCTTAG

Protein sequence

MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Homology
BLAST of MELO3C003651.jh1.t1 vs. NCBI nr
Match: XP_008466345.1 (PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Cucumis melo] >KAA0038775.1 aspartate--tRNA ligase 2 [Cucumis melo var. makuwa] >TYK31390.1 aspartate--tRNA ligase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. NCBI nr
Match: XP_004136326.1 (aspartate--tRNA ligase 2, cytoplasmic [Cucumis sativus] >KGN60145.1 hypothetical protein Csa_002506 [Cucumis sativus])

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEP SA D+SSKAVSKKAAKKEAAK EKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGR+QTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDE+FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNE+CKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. NCBI nr
Match: XP_023535979.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1018 bits (2631), Expect = 0.0
Identity = 516/545 (94.68%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEPLSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEA AE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. NCBI nr
Match: KAG6591612.1 (Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1016 bits (2628), Expect = 0.0
Identity = 515/545 (94.50%), Postives = 533/545 (97.80%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEPLSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELK+KYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKEKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. NCBI nr
Match: XP_022936995.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 1016 bits (2628), Expect = 0.0
Identity = 515/545 (94.50%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEPLSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. ExPASy Swiss-Prot
Match: Q9M084 (Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE=1 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 9.5e-215
Identity = 383/557 (68.76%), Postives = 446/557 (80.07%), Query Frame = 0

Query: 2   SASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQ---EAAAESAISSLNVEDD 61
           S S   P+    +A +ES +  SKKAAKKEAAKLEKLRRRQ   EA   +A  SL  E+D
Sbjct: 3   SESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-END 62

Query: 62  PLSANYGDVPLSDLQS-------KEVKSIE--NWTQVGSLTPELKDKYVILRGRIQTIRA 121
             S NYGDV L++LQS       K ++++E   WT V  L  E+ +  V++RGR+ T R 
Sbjct: 63  EFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRP 122

Query: 122 VGKKMAFLVVREKGFTVQCVLSEQPEL-VSRQMVKYVDGLSRESIVDVEGVVSVPNVAIK 181
              K+ F+V+RE G TVQCV+S+  +  V   MVKY+  LSRES VDV GVV++P   + 
Sbjct: 123 TSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLT 182

Query: 182 GASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRV 241
           G +QQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNYRV
Sbjct: 183 GTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRV 242

Query: 242 LDMRTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPA 301
           LD+RTPANQ IF++Q +V   FR+ L  ++FV IHTPKL+ GSSEGG++VFRL+YKGQPA
Sbjct: 243 LDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPA 302

Query: 302 CLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMD 361
           CLAQSPQLHKQMAICGD  RVFE+GPVFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD
Sbjct: 303 CLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMD 362

Query: 362 IVDRLFVAMFDSLNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDP 421
           +VD LFV +F SLNERCKK L+AV KQYPFEPLK+L KTLRLTFEEG+QMLK+AGVE+DP
Sbjct: 363 LVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDP 422

Query: 422 LGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEE 481
           LGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGEE
Sbjct: 423 LGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEE 482

Query: 482 IISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLN 541
           IISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC LN
Sbjct: 483 IISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALN 542

Query: 542 NIRKTSLFPRDPQRIAP 546
           NIRKTSLFPRDPQR++P
Sbjct: 543 NIRKTSLFPRDPQRLSP 558

BLAST of MELO3C003651.jh1.t1 vs. ExPASy Swiss-Prot
Match: Q8H104 (Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g26870 PE=2 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 9.6e-199
Identity = 354/531 (66.67%), Postives = 423/531 (79.66%), Query Frame = 0

Query: 17  DESSKAVSKKAAKKEAAKLEKLRRRQE-AAAESAISSLNVEDDPLSANYGDVPLSDLQSK 76
           +E  + +SKK +KK AAKLEKL R+QE   A S+  SL  ED+  S+NYGDV  ++LQS 
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQEREEATSSSLSLEEEDESCSSNYGDVTTNELQSA 67

Query: 77  -EVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVREKGFTVQCVLSEQP 136
            E K +   T V +L  E+    V +RGR+   R VG K+ F+++RE GFTVQCV+ E  
Sbjct: 68  VEGKEL---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE-- 127

Query: 137 ELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPIN 196
             V   M+K+V  LSRES+V++ GVVS P   + G +QQVEI VRK+YC+S+++P LP+ 
Sbjct: 128 TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLV 187

Query: 197 IEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFL 256
           +EDAARSE++IEK+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQCQV   FR++L
Sbjct: 188 VEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYL 247

Query: 257 LDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGP 316
             + F+EIHTPKLI GSSEGG++VFRLDYKGQPACLAQSPQLHKQMAICGD  RVFE+GP
Sbjct: 248 QSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGP 307

Query: 317 VFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNERCKKALEAVDK 376
           VFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD+V  LF  +F  + ERC K LE+V K
Sbjct: 308 VFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRK 367

Query: 377 QYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYI 436
           QYPF+ LK+L +TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLGQLVLEKY TEFY+
Sbjct: 368 QYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYM 427

Query: 437 LHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVK 496
           LHRYP AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDVK
Sbjct: 428 LHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVK 487

Query: 497 TIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           TI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 TISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of MELO3C003651.jh1.t1 vs. ExPASy Swiss-Prot
Match: Q5R9I5 (Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.5e-135
Identity = 258/508 (50.79%), Postives = 347/508 (68.31%), Query Frame = 0

Query: 39  RRRQEAAAESAISSLNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYV 98
           R+ QE   E     ++  +D     YG   +S +   + KS     +V  LT +  D+ V
Sbjct: 8   RKSQEKPRE----IMDAAEDYAKERYG---ISSMIQSQEKSDRVLVRVRDLTIQKADEVV 67

Query: 99  ILRGRIQTIRAVGKKMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEG 158
            +R R+ T RA GK+  FLV+R++ F VQ +++   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 159 VVSVPNVAIKGASQQ-VEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVR 218
           VV   N  I+  +QQ VE+ V+K+Y IS A P LP+ ++DA R EAE E+     E    
Sbjct: 128 VVRKVNQKIESCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 219 VNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGAS 278
           VNQDTRL+ RV+D+RT  +Q +FR+Q  +  +FR+ L+++ FVEI TPK+I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 279 VFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEM 338
           VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 339 EIKTHYSEVMDIVDRLFVAMFDSLNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQ 398
               HY EVM+ +    V +F  L ER +  ++ V+KQ+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 399 MLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYS 458
           ML++AGVE+    DL+T  E+ LG L+ EKY T+FYIL +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLIKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 459 NSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGL 518
           NS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 519 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of MELO3C003651.jh1.t1 vs. ExPASy Swiss-Prot
Match: P14868 (Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2)

HSP 1 Score: 483.4 bits (1243), Expect = 3.3e-135
Identity = 258/508 (50.79%), Postives = 345/508 (67.91%), Query Frame = 0

Query: 39  RRRQEAAAESAISSLNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYV 98
           R+ QE   E     ++  +D     YG   +S +   + K      +V  LT +  D+ V
Sbjct: 8   RKSQEKPRE----IMDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVRDLTIQKADEVV 67

Query: 99  ILRGRIQTIRAVGKKMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEG 158
            +R R+ T RA GK+  FLV+R++ F VQ +++   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 159 VVSVPNVAIKGASQQ-VEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVR 218
           VV   N  I   +QQ VE+ V+K+Y IS A P LP+ ++DA R EAE E+     E    
Sbjct: 128 VVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 219 VNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGAS 278
           VNQDTRL+ RV+D+RT  +Q +FR+Q  +  +FR+ L+++ FVEI TPK+I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 279 VFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEM 338
           VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 339 EIKTHYSEVMDIVDRLFVAMFDSLNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQ 398
               HY EVM+ +    V +F  L ER +  ++ V+KQ+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 399 MLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYS 458
           ML++AGVE+    DL+T  E+ LG LV EKY T+FYIL +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 459 NSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGL 518
           NS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 519 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of MELO3C003651.jh1.t1 vs. ExPASy Swiss-Prot
Match: Q922B2 (Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2)

HSP 1 Score: 480.7 bits (1236), Expect = 2.2e-134
Identity = 252/494 (51.01%), Postives = 340/494 (68.83%), Query Frame = 0

Query: 53  LNVEDDPLSANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGK 112
           ++  +D     YG   +S +   + K      +V  LT +  D  V +R R+ T RA GK
Sbjct: 18  VDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVKDLTVQKADDVVWVRARVHTSRAKGK 77

Query: 113 KMAFLVVREKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQ 172
           +  FLV+R++ F VQ +++   +  S+QMVK+   +++ESI+DVEGVV   N  I   +Q
Sbjct: 78  Q-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIIDVEGVVRKVNQKIGSCTQ 137

Query: 173 Q-VEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDM 232
           Q VE+ V+K+Y IS A P LP+ ++DA R E E E+  +A      VNQDTRL+ RV+D+
Sbjct: 138 QDVELHVQKIYVISLAEPRLPLQLDDAIRPEVEGEEDGRA-----TVNQDTRLDNRVIDL 197

Query: 233 RTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLA 292
           RT  +Q IFR+Q  +  +FR+ L+++ FVEI TPK+I  +SEGGA+VF + Y    A LA
Sbjct: 198 RTSTSQAIFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLA 257

Query: 293 QSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVD 352
           QSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM    HY EV++ + 
Sbjct: 258 QSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVEEIA 317

Query: 353 RLFVAMFDSLNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGD 412
              V +F  L ER +  ++ V KQ+P EP K+L  TLRL + E + ML++AGVE+D   D
Sbjct: 318 DTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMDDEED 377

Query: 413 LNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIIS 472
           L+T  E+ LG+LV EKY T+FY+L +YPLAVRPFYTMP   NP  SNS+D+F+RGEEI+S
Sbjct: 378 LSTPNEKLLGRLVKEKYDTDFYVLDKYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILS 437

Query: 473 GAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIR 532
           GAQR+H P+ L ERA   GID++ I+ YIDSFR+GAPPH G G+GLERV MLF GL+N+R
Sbjct: 438 GAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVR 497

Query: 533 KTSLFPRDPQRIAP 546
           +TS+FPRDP+R+ P
Sbjct: 498 QTSMFPRDPKRLTP 501

BLAST of MELO3C003651.jh1.t1 vs. ExPASy TrEMBL
Match: A0A5A7TBL8 (Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006980 PE=3 SV=1)

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. ExPASy TrEMBL
Match: A0A1S3CR09 (Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1)

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. ExPASy TrEMBL
Match: A0A0A0LDS7 (Aspartyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G881580 PE=3 SV=1)

HSP 1 Score: 1051 bits (2719), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASSQTPIDEP SA D+SSKAVSKKAAKKEAAK EKLRRRQEAAAESAISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGR+QTIRAVGKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQVSTMFRQFLLDE+FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LNE+CKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. ExPASy TrEMBL
Match: A0A6J1FFA5 (Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1)

HSP 1 Score: 1016 bits (2628), Expect = 0.0
Identity = 515/545 (94.50%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDEPLSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. ExPASy TrEMBL
Match: A0A6J1ILA3 (Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1)

HSP 1 Score: 1013 bits (2618), Expect = 0.0
Identity = 514/545 (94.31%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60
           MSASS+TPIDE LSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEAAAE+AISSLNVEDDPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300
           RIQCQV T+FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360
           +ICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHY EVMDIVDRLFV+MFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 545
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of MELO3C003651.jh1.t1 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 747.7 bits (1929), Expect = 6.8e-216
Identity = 383/557 (68.76%), Postives = 446/557 (80.07%), Query Frame = 0

Query: 2   SASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQ---EAAAESAISSLNVEDD 61
           S S   P+    +A +ES +  SKKAAKKEAAKLEKLRRRQ   EA   +A  SL  E+D
Sbjct: 3   SESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-END 62

Query: 62  PLSANYGDVPLSDLQS-------KEVKSIE--NWTQVGSLTPELKDKYVILRGRIQTIRA 121
             S NYGDV L++LQS       K ++++E   WT V  L  E+ +  V++RGR+ T R 
Sbjct: 63  EFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRP 122

Query: 122 VGKKMAFLVVREKGFTVQCVLSEQPEL-VSRQMVKYVDGLSRESIVDVEGVVSVPNVAIK 181
              K+ F+V+RE G TVQCV+S+  +  V   MVKY+  LSRES VDV GVV++P   + 
Sbjct: 123 TSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLT 182

Query: 182 GASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRV 241
           G +QQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNYRV
Sbjct: 183 GTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRV 242

Query: 242 LDMRTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPA 301
           LD+RTPANQ IF++Q +V   FR+ L  ++FV IHTPKL+ GSSEGG++VFRL+YKGQPA
Sbjct: 243 LDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPA 302

Query: 302 CLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMD 361
           CLAQSPQLHKQMAICGD  RVFE+GPVFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD
Sbjct: 303 CLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMD 362

Query: 362 IVDRLFVAMFDSLNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDP 421
           +VD LFV +F SLNERCKK L+AV KQYPFEPLK+L KTLRLTFEEG+QMLK+AGVE+DP
Sbjct: 363 LVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDP 422

Query: 422 LGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEE 481
           LGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGEE
Sbjct: 423 LGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEE 482

Query: 482 IISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLN 541
           IISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC LN
Sbjct: 483 IISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALN 542

Query: 542 NIRKTSLFPRDPQRIAP 546
           NIRKTSLFPRDPQR++P
Sbjct: 543 NIRKTSLFPRDPQRLSP 558

BLAST of MELO3C003651.jh1.t1 vs. TAIR 10
Match: AT4G31180.2 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 747.7 bits (1929), Expect = 6.8e-216
Identity = 383/557 (68.76%), Postives = 446/557 (80.07%), Query Frame = 0

Query: 2   SASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQ---EAAAESAISSLNVEDD 61
           S S   P+    +A +ES +  SKKAAKKEAAKLEKLRRRQ   EA   +A  SL  E+D
Sbjct: 3   SESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-END 62

Query: 62  PLSANYGDVPLSDLQS-------KEVKSIE--NWTQVGSLTPELKDKYVILRGRIQTIRA 121
             S NYGDV L++LQS       K ++++E   WT V  L  E+ +  V++RGR+ T R 
Sbjct: 63  EFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNRP 122

Query: 122 VGKKMAFLVVREKGFTVQCVLSEQPEL-VSRQMVKYVDGLSRESIVDVEGVVSVPNVAIK 181
              K+ F+V+RE G TVQCV+S+  +  V   MVKY+  LSRES VDV GVV++P   + 
Sbjct: 123 TSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPLT 182

Query: 182 GASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRV 241
           G +QQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNYRV
Sbjct: 183 GTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRV 242

Query: 242 LDMRTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPA 301
           LD+RTPANQ IF++Q +V   FR+ L  ++FV IHTPKL+ GSSEGG++VFRL+YKGQPA
Sbjct: 243 LDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQPA 302

Query: 302 CLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMD 361
           CLAQSPQLHKQMAICGD  RVFE+GPVFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD
Sbjct: 303 CLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIMD 362

Query: 362 IVDRLFVAMFDSLNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDP 421
           +VD LFV +F SLNERCKK L+AV KQYPFEPLK+L KTLRLTFEEG+QMLK+AGVE+DP
Sbjct: 363 LVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDP 422

Query: 422 LGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEE 481
           LGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGEE
Sbjct: 423 LGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEE 482

Query: 482 IISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLN 541
           IISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC LN
Sbjct: 483 IISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALN 542

Query: 542 NIRKTSLFPRDPQRIAP 546
           NIRKTSLFPRDPQR++P
Sbjct: 543 NIRKTSLFPRDPQRLSP 558

BLAST of MELO3C003651.jh1.t1 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 694.5 bits (1791), Expect = 6.8e-200
Identity = 354/531 (66.67%), Postives = 423/531 (79.66%), Query Frame = 0

Query: 17  DESSKAVSKKAAKKEAAKLEKLRRRQE-AAAESAISSLNVEDDPLSANYGDVPLSDLQSK 76
           +E  + +SKK +KK AAKLEKL R+QE   A S+  SL  ED+  S+NYGDV  ++LQS 
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQEREEATSSSLSLEEEDESCSSNYGDVTTNELQSA 67

Query: 77  -EVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVREKGFTVQCVLSEQP 136
            E K +   T V +L  E+    V +RGR+   R VG K+ F+++RE GFTVQCV+ E  
Sbjct: 68  VEGKEL---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE-- 127

Query: 137 ELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPIN 196
             V   M+K+V  LSRES+V++ GVVS P   + G +QQVEI VRK+YC+S+++P LP+ 
Sbjct: 128 TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLV 187

Query: 197 IEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFL 256
           +EDAARSE++IEK+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQCQV   FR++L
Sbjct: 188 VEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYL 247

Query: 257 LDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGP 316
             + F+EIHTPKLI GSSEGG++VFRLDYKGQPACLAQSPQLHKQMAICGD  RVFE+GP
Sbjct: 248 QSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGP 307

Query: 317 VFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNERCKKALEAVDK 376
           VFRAEDS+THRHLCEF GLDVEMEI+ HYSE+MD+V  LF  +F  + ERC K LE+V K
Sbjct: 308 VFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRK 367

Query: 377 QYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYI 436
           QYPF+ LK+L +TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLGQLVLEKY TEFY+
Sbjct: 368 QYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYM 427

Query: 437 LHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVK 496
           LHRYP AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDVK
Sbjct: 428 LHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVK 487

Query: 497 TIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           TI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 TISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of MELO3C003651.jh1.t1 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 2.8e-28
Identity = 87/272 (31.99%), Postives = 141/272 (51.84%), Query Frame = 0

Query: 282 DYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKT 341
           D+ G+ A L  S QL  +   C     V+  GP FRAE+S+T RHL EF  ++ E+    
Sbjct: 303 DFFGRQAFLTVSGQLQVETYACA-LSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAF-A 362

Query: 342 HYSEVMDIVDRLFVAMFDSLNERCKKALEAVDKQYP---FEPLKYLRKTL--RLTFEEGI 401
              + M+  +     M + L E+C   +E + K +     + LK +  T   R+T+ + I
Sbjct: 363 DLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAI 422

Query: 402 QMLKDA---GVEID---PLG-DLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMP 461
           ++L++A   G E D     G DL +E ER L +++ +K      I++ YP  ++ FY M 
Sbjct: 423 ELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKP----LIVYNYPKGIKAFY-MR 482

Query: 462 CYDNPTYSNSFDVFI-RGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAP 521
             D+     + DV + +  E+I G+QR    + + +R +  G+ ++  E Y+D  RYG  
Sbjct: 483 LNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTV 542

Query: 522 PHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 541
            H GFG+G ER+++   GL+NIR    FPR P
Sbjct: 543 KHCGFGLGFERMILFATGLDNIRDVIPFPRYP 567

BLAST of MELO3C003651.jh1.t1 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 4.8e-28
Identity = 119/482 (24.69%), Postives = 204/482 (42.32%), Query Frame = 0

Query: 100 LRGRIQTIRAVGKKMAFLVVREKG--FTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVE 159
           + G ++T+R+    + F+ + +      +QCV++   E      V+    L+  S V V+
Sbjct: 114 IMGWVRTLRS-QSSVTFIEINDGSCLSNLQCVMTSDAE--GYDQVESGSILTGAS-VSVQ 173

Query: 160 GVVSVPNVAIKGASQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVR 219
           G +    VA +G  Q+VE++V K+  + +   + PI                    Q  R
Sbjct: 174 GTI----VASQGTKQKVELKVEKIIVVGECDSSYPI--------------------QKKR 233

Query: 220 VNQDTRLNYRVLDMRTPANQGIFRIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGAS 279
           V+++       L  RT     + R++  ++    +F  +  FV + +P +     EG   
Sbjct: 234 VSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIITASDCEGAGE 293

Query: 280 VFRL------------------------------DYKGQPACLAQSPQLHKQMAICGDFG 339
            F +                              D+ G+PA L  S QL+ +        
Sbjct: 294 QFCVTTLIPSSHENTDTSIDAIPKTKGGLIDWSQDFFGKPAFLTVSGQLNGE-TYATALS 353

Query: 340 RVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDSLNERCKK 399
            V+  GP FRAE+S T RHL EF  ++ E+       + M         +   + + CK+
Sbjct: 354 DVYTFGPTFRAENSNTSRHLAEFWMIEPELAF-ADLDDDMACATAYLQYVVKYVLDNCKE 413

Query: 400 ALEAVDKQYPFEPLKYL-----RKTLRLTFEEGIQMLKDAGVEID-PLG---DLNTEAER 459
            +E  D       ++ L     ++ L+L + + I++L  A  + D P+    DL +E ER
Sbjct: 414 DMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKKFDFPVKWGLDLQSEHER 473

Query: 460 KLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHV 519
            + +   E +G    I+  YP  ++ FY     D  T +    +  R  E+I G+QR   
Sbjct: 474 YITE---EAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIGGSQREER 533

Query: 520 PEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPR 541
            E L  R     ++ ++   Y+D  RYG+ PH GFG+G ER+V    G++NIR    FPR
Sbjct: 534 LEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFPR 562

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008466345.10.0100.00PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Cucumis melo] >KAA0038775.1 as... [more]
XP_004136326.10.098.53aspartate--tRNA ligase 2, cytoplasmic [Cucumis sativus] >KGN60145.1 hypothetical... [more]
XP_023535979.10.094.68aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
KAG6591612.10.094.50Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022936995.10.094.50aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M0849.5e-21568.76Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE... [more]
Q8H1049.6e-19966.67Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g268... [more]
Q5R9I51.5e-13550.79Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1[more]
P148683.3e-13550.79Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2[more]
Q922B22.2e-13451.01Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7TBL80.0100.00Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CR090.0100.00Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1[more]
A0A0A0LDS70.098.53Aspartyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G881580 PE=3 SV=1[more]
A0A6J1FFA50.094.50Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1[more]
A0A6J1ILA30.094.31Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31180.16.8e-21668.76Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G31180.26.8e-21668.76Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G26870.16.8e-20066.67Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT5G56680.12.8e-2831.99Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.14.8e-2824.69Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 25..48
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 192..545
e-value: 4.8E-130
score: 435.8
NoneNo IPR availableGENE3D2.40.50.140coord: 56..190
e-value: 1.8E-39
score: 136.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR43450:SF1ASPARTATE--TRNA LIGASE, CYTOPLASMICcoord: 16..545
NoneNo IPR availableCDDcd04320AspRS_cyto_Ncoord: 97..197
e-value: 1.22512E-44
score: 151.177
NoneNo IPR availableCDDcd00776AsxRS_corecoord: 217..541
e-value: 2.08352E-166
score: 472.821
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 215..544
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 290..302
score: 55.24
coord: 307..320
score: 51.79
coord: 500..514
score: 55.15
coord: 460..476
score: 40.11
IPR004523Aspartate-tRNA synthetase, type 2TIGRFAMTIGR00458TIGR00458coord: 89..545
e-value: 4.7E-143
score: 475.2
IPR004523Aspartate-tRNA synthetase, type 2PANTHERPTHR43450ASPARTYL-TRNA SYNTHETASEcoord: 16..545
IPR004523Aspartate-tRNA synthetase, type 2HAMAPMF_02075Asp_tRNA_synth_type2coord: 81..545
score: 39.755821
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 220..539
e-value: 1.2E-76
score: 257.8
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 98..182
e-value: 1.5E-7
score: 31.3
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 240..537
score: 18.947845
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 62..190

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C003651.jh1MELO3C003651.jh1gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exonexon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_2exon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_3exon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_4exon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_5exon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_6exon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_7exon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_8exon
MELO3C003651.jh1.t1.exonMELO3C003651.jh1.t1.exon_9exon


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C003651.jh1.t1.stop_codonMELO3C003651.jh1.t1.stop_codonstop_codon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cdsCDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_2CDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_3CDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_4CDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_5CDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_6CDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_7CDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_8CDS
MELO3C003651.jh1.t1.cdsMELO3C003651.jh1.t1.cds_9CDS


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C003651.jh1.t1.start_codonMELO3C003651.jh1.t1.start_codonstart_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C003651.jh1.t1MELO3C003651.jh1.t1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006422 aspartyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0004815 aspartate-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding