MC11g1320.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1320.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	22.0 kDa class IV heat shock protein
Location	MC11: 16027857 .. 16029282 (-)
Sequence length	684
RNA-Seq Expression	MC11g1320.1
Synteny	MC11g1320.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAAAGCAATGACAATGACAATGAGAGCAATACAAATGGGCTTGTGCTCACTATTCATCATCTTCTTTTGTCTCATAAATGGCTCAGCAAACGCCTCTCTGCTGCCATTACTGGATCCATTTGGGGTGCTAGAACAAACCCCATTGGGCCTCCAAAAGGAACATGCAAACGGAGATCCTCTGCTCCTCCTCGCCCCGGCGAGAGCCGACTGGAAGGAGACGTCGGAGGGCCACGTCATCAGGATGGACGTGCCCGGGCTGGCCAAGGAGGAGCTGAGGATTGAGGTGGATGACAACAGGATTCTCAAGGTAGAGTATTAAATTTTCATCCACATTCTATGAGATGTTAAAAAATAAAATATCTTTGGCTATGTAATTTCGTTAAGCTAGAAATTTAATCTTTAGATTTTTGTTTGTAAGTAATGATTTTATTCTCTATATTTTCAAGTGGGTAAGACTGAAATCTTATTAAAACTGTTAATTTGTGTCACAATTTATAAATAAATTTGACCATTTGCAGTAATGTTGATGTTTTTTATTTTTATTTTTTTATTAAAAAAAGAAAAGAAAAATTGTATAGCCGATCTCGCCATGCATTTAAGCATGATAAAACAATATTAAATTTTTATACAATTTTAAGAATGTTAAAAAAAATGAGAAACTAATAGTGATTTCCGGAGAAATTTAGAAACGGGAACGTAATCAAGCTATCTATGATTAGTTGCAAAACGTCGAGAATTTTAATAAAATTGAAAAAGAAATAAAAAGACAATAACGTTAGCAAAGTGGTTCTCATCAACTCACATGTATAAGAAATTTAAGACAAAAAATTTATAATAGAAAATAATCGTCCATAATATTTTCGTTTCTTATTTCTCGTTTCCAATTTTTTTAACAAATCTAAATATTTTGGTCACTAACTATGTTTTTTATTCTTCTCTTTATATCAAAATTCGTACAAGTTTATTTTTTTACTCAATTGAGTGTCGAAATCATGCAATTAATTTTTGTTTATTAAAAATGAAGAAAAATCTATAAACTTTGTTGTCAGGTGATGGGAGAGAGGAAAAGGGAAGAGGAGAGAGAAGGAGACCACTGGCACCGCGTGGAGAGAAGTTACGGAAAGTTCTGGAGGCAGTTTCGGATGCCGAGTGACGTGGATTTGGACTCCGTTAAAGCTACGCTTGAGAACGGCGTTCTCACCGTTACTCTGCCCAAACTTTCGCCGGAGAAGATCAAGGGTCCCAAACTCGTCGGAATTTCTGATCACAAATCGACGGCCGAGATCCAGTCCAAGCAGGAGCTGTGACTCATCTCTTTTCTTTTACCTTCAGTTCAGTTATGGCGCATGTTTGAGATGTTGTACGTGTACGTGTACCTGTACGGGTGAATAAAGAAGCACGTTTGTTATCTAAAAGAAATTTAC

mRNA sequence

ATCAAAGCAATGACAATGACAATGAGAGCAATACAAATGGGCTTGTGCTCACTATTCATCATCTTCTTTTGTCTCATAAATGGCTCAGCAAACGCCTCTCTGCTGCCATTACTGGATCCATTTGGGGTGCTAGAACAAACCCCATTGGGCCTCCAAAAGGAACATGCAAACGGAGATCCTCTGCTCCTCCTCGCCCCGGCGAGAGCCGACTGGAAGGAGACGTCGGAGGGCCACGTCATCAGGATGGACGTGCCCGGGCTGGCCAAGGAGGAGCTGAGGATTGAGGTGGATGACAACAGGATTCTCAAGGTGATGGGAGAGAGGAAAAGGGAAGAGGAGAGAGAAGGAGACCACTGGCACCGCGTGGAGAGAAGTTACGGAAAGTTCTGGAGGCAGTTTCGGATGCCGAGTGACGTGGATTTGGACTCCGTTAAAGCTACGCTTGAGAACGGCGTTCTCACCGTTACTCTGCCCAAACTTTCGCCGGAGAAGATCAAGGGTCCCAAACTCGTCGGAATTTCTGATCACAAATCGACGGCCGAGATCCAGTCCAAGCAGGAGCTGTGACTCATCTCTTTTCTTTTACCTTCAGTTCAGTTATGGCGCATGTTTGAGATGTTGTACGTGTACGTGTACCTGTACGGGTGAATAAAGAAGCACGTTTGTTATCTAAAAGAAATTTAC

Coding sequence (CDS)

ATGACAATGACAATGAGAGCAATACAAATGGGCTTGTGCTCACTATTCATCATCTTCTTTTGTCTCATAAATGGCTCAGCAAACGCCTCTCTGCTGCCATTACTGGATCCATTTGGGGTGCTAGAACAAACCCCATTGGGCCTCCAAAAGGAACATGCAAACGGAGATCCTCTGCTCCTCCTCGCCCCGGCGAGAGCCGACTGGAAGGAGACGTCGGAGGGCCACGTCATCAGGATGGACGTGCCCGGGCTGGCCAAGGAGGAGCTGAGGATTGAGGTGGATGACAACAGGATTCTCAAGGTGATGGGAGAGAGGAAAAGGGAAGAGGAGAGAGAAGGAGACCACTGGCACCGCGTGGAGAGAAGTTACGGAAAGTTCTGGAGGCAGTTTCGGATGCCGAGTGACGTGGATTTGGACTCCGTTAAAGCTACGCTTGAGAACGGCGTTCTCACCGTTACTCTGCCCAAACTTTCGCCGGAGAAGATCAAGGGTCCCAAACTCGTCGGAATTTCTGATCACAAATCGACGGCCGAGATCCAGTCCAAGCAGGAGCTGTGA

Protein sequence

MTMTMRAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHWHRVERSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTAEIQSKQEL

Homology

[Close]

BLAST of MC11g1320.1 vs. ExPASy Swiss-Prot
Match: P30236 (22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.6e-51
Identity = 104/183 (56.83%), Postives = 131/183 (71.58%), Query Frame = 0


Query: 10  MGLCSLFIIFFCLINGSANASLLPLL----------------DPFGVLEQTPLGLQKEHA 69
           M L  L + F  L    AN SLLP +                DPF VLE  P G+ K+ A
Sbjct: 1   MRLQQLNLFFLLLCVAKANGSLLPFMDPPITLLADLWSDRFPDPFRVLEHIPFGVDKDEA 60

Query: 70  NGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREG 129
           +    + ++PAR DWKET EGHVI +DVPGL +EE+++EV++NR+L+V GERK+EEE++G
Sbjct: 61  S----MAMSPARVDWKETPEGHVIMLDVPGLKREEIKVEVEENRVLRVSGERKKEEEKKG 120

Query: 130 DHWHRVERSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGIS-- 175
           DHWHRVERSYGKFWRQFR+P +VDLDSVKA LENGVLT+TL KLSP KIKGP++V I+  
Sbjct: 121 DHWHRVERSYGKFWRQFRLPQNVDLDSVKAKLENGVLTLTLDKLSPGKIKGPRVVSIAGE 179

[Close]

BLAST of MC11g1320.1 vs. ExPASy Swiss-Prot
Match: Q38806 (22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.2e-48
Identity = 90/149 (60.40%), Postives = 121/149 (81.21%), Query Frame = 0


Query: 36  DPFGVLEQTPLGLQKEHANGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELRIEVDD 95
           DPF +LE+ PLGL++     D  + L+PAR DWKET+EGH I +D+PGL K+E++IEV++
Sbjct: 48  DPFKILERIPLGLER-----DTSVALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEE 107

Query: 96  NRILKVMGERKREEEREGDHWHRVERSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLP 155
           N +L+V GERKREEE++GD WHRVERSYGKFWRQF++P +VD++SVKA LENGVLT+ L 
Sbjct: 108 NGVLRVSGERKREEEKKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKLENGVLTINLT 167

Query: 156 KLSPEKIKGPKLVGI-SDHKSTAEIQSKQ 184
           KLSPEK+KGP++V I ++   TA+I S +
Sbjct: 168 KLSPEKVKGPRVVNIAAEEDQTAKISSSE 191

[Close]

BLAST of MC11g1320.1 vs. ExPASy Swiss-Prot
Match: P19244 (22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.2e-47
Identity = 94/160 (58.75%), Postives = 121/160 (75.62%), Query Frame = 0


Query: 27  ANASLLPLL----------------DPFGVLEQTPLGLQKEHANGDPLLLLAPARADWKE 86
           A ASLLP +                DPF VLEQ P G++K     +P + L+ AR DWKE
Sbjct: 26  AKASLLPFIDSPNTLLSDLWSDRFPDPFRVLEQIPYGVEKH----EPSITLSHARVDWKE 85

Query: 87  TSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHWHRVERSYGKFWRQF 146
           T EGHVI +DVPGL K++++IEV++NR+L+V GERK+EE+++GDHWHRVERSYGKFWRQF
Sbjct: 86  TPEGHVIMVDVPGLKKDDIKIEVEENRVLRVSGERKKEEDKKGDHWHRVERSYGKFWRQF 145

Query: 147 RMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGI 171
           ++P +VDLDSVKA +ENGVLT+TL KLS +KIKGP++V I
Sbjct: 146 KLPQNVDLDSVKAKMENGVLTLTLHKLSHDKIKGPRMVSI 181

[Close]

BLAST of MC11g1320.1 vs. ExPASy Swiss-Prot
Match: Q7XUW5 (23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 PE=2 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 1.4e-40
Identity = 78/145 (53.79%), Postives = 107/145 (73.79%), Query Frame = 0


Query: 31  LLPLLDPFGVLEQTPLGLQKEHANGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELR 90
           LL   DPF +LE  P G  +     D + +L+ AR DW+ET + H + +DVPG+ KE+LR
Sbjct: 48  LLAAADPFRILEHVPFGFDR-----DDVAMLSMARVDWRETGDAHEVVVDVPGMRKEDLR 107

Query: 91  IEVDDNRILKVMGERKREEERE----GDHWHRVERSYGKFWRQFRMPSDVDLDSVKATLE 150
           +EV+DNR+L++ GER+REE  E    GDHWHR ERSYG+FWRQ R+P + DLDS+ A+L+
Sbjct: 108 VEVEDNRVLRISGERRREETTEQKGGGDHWHREERSYGRFWRQLRLPDNADLDSIAASLD 167

Query: 151 NGVLTVTLPKLSPEKIKGPKLVGIS 172
           NGVLTV   KL+P++IKGP++VGI+
Sbjct: 168 NGVLTVRFRKLAPDQIKGPRVVGIA 187

[Close]

BLAST of MC11g1320.1 vs. ExPASy Swiss-Prot
Match: Q53M11 (21.9 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP21.9 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-30
Identity = 71/147 (48.30%), Postives = 100/147 (68.03%), Query Frame = 0


Query: 36  DPFGVLEQTPL----GLQKEHANGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELRI 95
           DPF VLEQ+PL    G+    A G+P   +A AR DWKET E HV+ +DVPG+ + ++R+
Sbjct: 41  DPFRVLEQSPLRPAGGVAAAAAAGEP-AAVALARCDWKETPEAHVVTVDVPGVRRGDVRV 100

Query: 96  EVDD-NRILKVMGERKR-------EEEREGDHWHRVERSYGKFWRQFRMPSDVDLDSVKA 155
           EVD+ +R+L+V GER+R       E ER+G  WHR ER+ G+FWR+FRMP   D+  V A
Sbjct: 101 EVDEASRVLRVSGERRRAGAAEEEEGERDGVRWHRAERAAGRFWRRFRMPPGADVGRVAA 160

Query: 156 TLENGVLTVTLPKLSPEKIKGPKLVGI 171
            L++GVLTVT+PK+   + + P++V I
Sbjct: 161 RLDDGVLTVTVPKVPGHRGREPRVVAI 186

[Close]

BLAST of MC11g1320.1 vs. NCBI nr
Match: XP_022156025.1 (22.0 kDa class IV heat shock protein [Momordica charantia])

HSP 1 Score: 360 bits (923), Expect = 4.22e-125
Identity = 184/185 (99.46%), Postives = 184/185 (99.46%), Query Frame = 0


Query: 1   MTMTMRAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL 60
           MTMTMRAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL
Sbjct: 1   MTMTMRAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL 60

Query: 61  LAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHWHRVE 120
           LAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMG RKREEEREGDHWHRVE
Sbjct: 61  LAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGXRKREEEREGDHWHRVE 120

Query: 121 RSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTAEIQ 180
           RSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTAEIQ
Sbjct: 121 RSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTAEIQ 180

Query: 181 SKQEL 185
           SKQEL
Sbjct: 181 SKQEL 185

[Close]

BLAST of MC11g1320.1 vs. NCBI nr
Match: ADU55793.1 (HSP20.3 [Citrullus lanatus])

HSP 1 Score: 204 bits (518), Expect = 1.52e-63
Identity = 109/174 (62.64%), Postives = 137/174 (78.74%), Query Frame = 0


Query: 6   RAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL-LAPA 65
           +A+Q+ L ++FI  F L    +  SLLP +DPFG+LEQTP GL  E+ N D L   L PA
Sbjct: 5   KAVQLFLFAIFISSFLL--TPSEPSLLPFIDPFGILEQTPFGLL-ENENRDALQQPLPPA 64

Query: 66  RADWKETSEGHVIRMDVPGLAKEELRIEVDD-NRILKVMGERKREEEREGDHWHRVERSY 125
           R DWKET E HVI +DVPG+ KEE++IE+D+ NRILKV+GERKREEE++ +HWHR+ERSY
Sbjct: 65  RVDWKETPESHVIMLDVPGMNKEEMKIELDEENRILKVIGERKREEEKQSEHWHRLERSY 124

Query: 126 GKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTA 177
           GKFWRQFR+PS+ D++SVKA L+NGVL VTL KLSPEKIKGP++VGI D +  A
Sbjct: 125 GKFWRQFRLPSNADMESVKAQLQNGVLKVTLSKLSPEKIKGPRVVGILDEQPPA 175

[Close]

BLAST of MC11g1320.1 vs. NCBI nr
Match: XP_008459498.1 (PREDICTED: 22.0 kDa class IV heat shock protein [Cucumis melo])

HSP 1 Score: 202 bits (513), Expect = 1.29e-62
Identity = 114/188 (60.64%), Postives = 142/188 (75.53%), Query Frame = 0


Query: 6   RAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL--LAP 65
           +AIQ+   ++FI  F  +  S+  SLLP +DPFG+LEQTP GL  E+ N D L    L P
Sbjct: 5   KAIQLIFSTVFI--FAFLITSSQPSLLPFIDPFGILEQTPFGLL-ENENKDALQQQPLPP 64

Query: 66  ARADWKETSEGHVIRMDVPGLAKEELRIEVDD-NRILKVMGERKREEEREGDHWHRVERS 125
           AR DWKET+E H I +DVPG+ KEEL+IE+D+ NR+LKV+GERKREEE++ DHWHR+ERS
Sbjct: 65  ARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLERS 124

Query: 126 YGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTA-EIQ- 185
           YGKFWRQ R+P + DL+SVKA LENGVL +TL KLSPEKIKGP++VGI D +  A EI  
Sbjct: 125 YGKFWRQLRLPVNADLESVKAKLENGVLKITLSKLSPEKIKGPRVVGILDEQPPAGEINK 184

[Close]

BLAST of MC11g1320.1 vs. NCBI nr
Match: KAA0039359.1 (22.0 kDa class IV heat shock protein [Cucumis melo var. makuwa] >TYK00542.1 22.0 kDa class IV heat shock protein [Cucumis melo var. makuwa])

HSP 1 Score: 202 bits (513), Expect = 1.29e-62
Identity = 114/188 (60.64%), Postives = 143/188 (76.06%), Query Frame = 0


Query: 6   RAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL--LAP 65
           +AIQ+   ++FI  F  +  S+  SLLP +DPFG+LEQTP GL  E+ N D L    L P
Sbjct: 5   KAIQLIFSTVFI--FAFLITSSQPSLLPFIDPFGILEQTPFGLL-ENENKDALQQQPLPP 64

Query: 66  ARADWKETSEGHVIRMDVPGLAKEELRIEVDD-NRILKVMGERKREEEREGDHWHRVERS 125
           AR DWKET+E H I +DVPG+ KEEL+IE+D+ NR+LKV+GERKREEE++ DHWHR+ERS
Sbjct: 65  ARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLERS 124

Query: 126 YGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTA-EIQ- 185
           YGKFWRQ R+P + DL+SVKA LENGVL +TL KLSPEKIKGP++VGI D ++ A EI  
Sbjct: 125 YGKFWRQLRLPVNADLESVKAKLENGVLKITLSKLSPEKIKGPRVVGILDGQTPAGEINK 184

[Close]

BLAST of MC11g1320.1 vs. NCBI nr
Match: XP_042969442.1 (22.0 kDa class IV heat shock protein [Carya illinoinensis] >KAG6659123.1 hypothetical protein CIPAW_03G011300 [Carya illinoinensis] >KAG6719445.1 hypothetical protein I3842_03G006000 [Carya illinoinensis] >KAG7985153.1 hypothetical protein I3843_03G010400 [Carya illinoinensis])

HSP 1 Score: 201 bits (510), Expect = 3.35e-62
Identity = 104/163 (63.80%), Postives = 127/163 (77.91%), Query Frame = 0


Query: 21  CLINGSANASLLPLL------------DPFGVLEQTPLGLQKEHANGDPLLLLAPARADW 80
           C +   +N SLLPL+            DPF VLEQ P+GL KE    D  L L+PAR DW
Sbjct: 16  CTLVRFSNGSLLPLIERPGSMLADIWTDPFRVLEQIPVGLDKE----DSALALSPARVDW 75

Query: 81  KETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHWHRVERSYGKFWR 140
           KET EGH+I++DVPGL +E+L+IEVDD R+L+V GERKREEE+EG+HWHR+ERSYGKFWR
Sbjct: 76  KETPEGHLIKLDVPGLKQEDLKIEVDD-RVLRVSGERKREEEKEGEHWHRMERSYGKFWR 135

Query: 141 QFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGIS 171
           QFR+P +VDLDSVKA LENGVLT+TLPKLSP+KIK P++V I+
Sbjct: 136 QFRLPENVDLDSVKAKLENGVLTLTLPKLSPDKIKSPRVVSIA 173

[Close]

BLAST of MC11g1320.1 vs. ExPASy TrEMBL
Match: A0A6J1DP53 (22.0 kDa class IV heat shock protein OS=Momordica charantia OX=3673 GN=LOC111022995 PE=3 SV=1)

HSP 1 Score: 360 bits (923), Expect = 2.04e-125
Identity = 184/185 (99.46%), Postives = 184/185 (99.46%), Query Frame = 0


Query: 1   MTMTMRAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL 60
           MTMTMRAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL
Sbjct: 1   MTMTMRAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL 60

Query: 61  LAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHWHRVE 120
           LAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMG RKREEEREGDHWHRVE
Sbjct: 61  LAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGXRKREEEREGDHWHRVE 120

Query: 121 RSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTAEIQ 180
           RSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTAEIQ
Sbjct: 121 RSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTAEIQ 180

Query: 181 SKQEL 185
           SKQEL
Sbjct: 181 SKQEL 185

[Close]

BLAST of MC11g1320.1 vs. ExPASy TrEMBL
Match: H6TB46 (HSP20.3 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 204 bits (518), Expect = 7.38e-64
Identity = 109/174 (62.64%), Postives = 137/174 (78.74%), Query Frame = 0


Query: 6   RAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL-LAPA 65
           +A+Q+ L ++FI  F L    +  SLLP +DPFG+LEQTP GL  E+ N D L   L PA
Sbjct: 5   KAVQLFLFAIFISSFLL--TPSEPSLLPFIDPFGILEQTPFGLL-ENENRDALQQPLPPA 64

Query: 66  RADWKETSEGHVIRMDVPGLAKEELRIEVDD-NRILKVMGERKREEEREGDHWHRVERSY 125
           R DWKET E HVI +DVPG+ KEE++IE+D+ NRILKV+GERKREEE++ +HWHR+ERSY
Sbjct: 65  RVDWKETPESHVIMLDVPGMNKEEMKIELDEENRILKVIGERKREEEKQSEHWHRLERSY 124

Query: 126 GKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTA 177
           GKFWRQFR+PS+ D++SVKA L+NGVL VTL KLSPEKIKGP++VGI D +  A
Sbjct: 125 GKFWRQFRLPSNADMESVKAQLQNGVLKVTLSKLSPEKIKGPRVVGILDEQPPA 175

[Close]

BLAST of MC11g1320.1 vs. ExPASy TrEMBL
Match: A0A5A7TDL3 (22.0 kDa class IV heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001530 PE=3 SV=1)

HSP 1 Score: 202 bits (513), Expect = 6.25e-63
Identity = 114/188 (60.64%), Postives = 143/188 (76.06%), Query Frame = 0


Query: 6   RAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL--LAP 65
           +AIQ+   ++FI  F  +  S+  SLLP +DPFG+LEQTP GL  E+ N D L    L P
Sbjct: 5   KAIQLIFSTVFI--FAFLITSSQPSLLPFIDPFGILEQTPFGLL-ENENKDALQQQPLPP 64

Query: 66  ARADWKETSEGHVIRMDVPGLAKEELRIEVDD-NRILKVMGERKREEEREGDHWHRVERS 125
           AR DWKET+E H I +DVPG+ KEEL+IE+D+ NR+LKV+GERKREEE++ DHWHR+ERS
Sbjct: 65  ARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLERS 124

Query: 126 YGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTA-EIQ- 185
           YGKFWRQ R+P + DL+SVKA LENGVL +TL KLSPEKIKGP++VGI D ++ A EI  
Sbjct: 125 YGKFWRQLRLPVNADLESVKAKLENGVLKITLSKLSPEKIKGPRVVGILDGQTPAGEINK 184

[Close]

BLAST of MC11g1320.1 vs. ExPASy TrEMBL
Match: A0A1S3CAU1 (22.0 kDa class IV heat shock protein OS=Cucumis melo OX=3656 GN=LOC103498616 PE=3 SV=1)

HSP 1 Score: 202 bits (513), Expect = 6.25e-63
Identity = 114/188 (60.64%), Postives = 142/188 (75.53%), Query Frame = 0


Query: 6   RAIQMGLCSLFIIFFCLINGSANASLLPLLDPFGVLEQTPLGLQKEHANGDPLLL--LAP 65
           +AIQ+   ++FI  F  +  S+  SLLP +DPFG+LEQTP GL  E+ N D L    L P
Sbjct: 5   KAIQLIFSTVFI--FAFLITSSQPSLLPFIDPFGILEQTPFGLL-ENENKDALQQQPLPP 64

Query: 66  ARADWKETSEGHVIRMDVPGLAKEELRIEVDD-NRILKVMGERKREEEREGDHWHRVERS 125
           AR DWKET+E H I +DVPG+ KEEL+IE+D+ NR+LKV+GERKREEE++ DHWHR+ERS
Sbjct: 65  ARVDWKETAESHEIMVDVPGMNKEELKIELDEENRVLKVIGERKREEEKQSDHWHRLERS 124

Query: 126 YGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGISDHKSTA-EIQ- 185
           YGKFWRQ R+P + DL+SVKA LENGVL +TL KLSPEKIKGP++VGI D +  A EI  
Sbjct: 125 YGKFWRQLRLPVNADLESVKAKLENGVLKITLSKLSPEKIKGPRVVGILDEQPPAGEINK 184

[Close]

BLAST of MC11g1320.1 vs. ExPASy TrEMBL
Match: I1LC12 (SHSP domain-containing protein OS=Glycine max OX=3847 GN=GLYMA_10G176400 PE=3 SV=1)

HSP 1 Score: 200 bits (508), Expect = 4.35e-62
Identity = 103/175 (58.86%), Postives = 132/175 (75.43%), Query Frame = 0


Query: 14  SLFIIFFCLIN-GSANASLLPLLDP----------------FGVLEQTPLGLQKEHANGD 73
           +LF++ F L+    AN SLLP +DP                F VLEQ P G+ K+    +
Sbjct: 7   NLFLVSFLLLFVARANGSLLPFIDPPTTLLADLWSDRFPDPFRVLEQIPFGVDKD----E 66

Query: 74  PLLLLAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHW 133
           P + ++PAR DWKET EGHVI +DVPGL +EE++IEV++NR+L+V GERK+EEE++GDHW
Sbjct: 67  PSMAMSPARVDWKETPEGHVIMLDVPGLKREEIKIEVEENRVLRVSGERKKEEEKKGDHW 126

Query: 134 HRVERSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGIS 171
           HRVERSYGKFWRQFR+P +VDLDSVKA +ENGVLT+TL KLSP+KIKGP+LV I+
Sbjct: 127 HRVERSYGKFWRQFRLPQNVDLDSVKAKMENGVLTLTLDKLSPDKIKGPRLVSIA 177

[Close]

BLAST of MC11g1320.1 vs. TAIR 10
Match: AT4G10250.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 3.7e-49
Identity = 90/149 (60.40%), Postives = 121/149 (81.21%), Query Frame = 0


Query: 36  DPFGVLEQTPLGLQKEHANGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELRIEVDD 95
           DPF +LE+ PLGL++     D  + L+PAR DWKET+EGH I +D+PGL K+E++IEV++
Sbjct: 48  DPFKILERIPLGLER-----DTSVALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEE 107

Query: 96  NRILKVMGERKREEEREGDHWHRVERSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLP 155
           N +L+V GERKREEE++GD WHRVERSYGKFWRQF++P +VD++SVKA LENGVLT+ L 
Sbjct: 108 NGVLRVSGERKREEEKKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKLENGVLTINLT 167

Query: 156 KLSPEKIKGPKLVGI-SDHKSTAEIQSKQ 184
           KLSPEK+KGP++V I ++   TA+I S +
Sbjct: 168 KLSPEKVKGPRVVNIAAEEDQTAKISSSE 191

[Close]

BLAST of MC11g1320.1 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 59/108 (54.63%), Postives = 80/108 (74.07%), Query Frame = 0


Query: 64  ARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHWHRVERSY 123
           AR DWKET E HV + D+PGL KEE+++EV+D  +L++ GER +E E + D WHRVER+ 
Sbjct: 52  ARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERAS 111

Query: 124 GKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIKGPKLVGIS 172
           GKF R+FR+P +  ++ VKAT+ENGVLTV +PK +PEK    K + IS
Sbjct: 112 GKFMRRFRLPENAKMEEVKATMENGVLTVVVPK-APEKKPQVKSIDIS 158

[Close]

BLAST of MC11g1320.1 vs. TAIR 10
Match: AT1G53540.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-29
Identity = 68/141 (48.23%), Postives = 94/141 (66.67%), Query Frame = 0


Query: 32  LPLLDPF-GVLEQTPLGLQKEHANGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELR 91
           L + DPF G L  TP GL   +A    +     A+ DW+ET E HV + D+PGL KEE++
Sbjct: 21  LDVFDPFEGFL--TPSGL--ANAPAMDVAAFTNAKVDWRETPEAHVFKADLPGLRKEEVK 80

Query: 92  IEVDDNRILKVMGERKREEEREGDHWHRVERSYGKFWRQFRMPSDVDLDSVKATLENGVL 151
           +EV+D  IL++ GER  E E + D WHRVERS GKF R+FR+P +  ++ +KA++ENGVL
Sbjct: 81  VEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKMEEIKASMENGVL 140

Query: 152 TVTLPKLSPEKIKGPKLVGIS 172
           +VT+PK+ PEK    K + IS
Sbjct: 141 SVTVPKV-PEKKPEVKSIDIS 156

[Close]

BLAST of MC11g1320.1 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 2.5e-29
Identity = 64/132 (48.48%), Postives = 91/132 (68.94%), Query Frame = 0


Query: 32  LPLLDPFGVLEQTPLGLQKEHANGDPLLLLAPARADWKETSEGHVIRMDVPGLAKEELRI 91
           L + DPF  L Q P  L  E +       +  AR DWKET+E HV + D+PG+ KEE+++
Sbjct: 23  LDVWDPFKEL-QFPSSLSGETS------AITNARVDWKETAEAHVFKADLPGMKKEEVKV 82

Query: 92  EVDDNRILKVMGERKREEEREGDHWHRVERSYGKFWRQFRMPSDVDLDSVKATLENGVLT 151
           E++D+ +LK+ GER  E+E + D WHRVERS G+F R+F++P +V +D VKA++ENGVLT
Sbjct: 83  EIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASMENGVLT 142

Query: 152 VTLPKLSPEKIK 164
           VT+PK+   K K
Sbjct: 143 VTVPKVEEAKKK 147

[Close]

BLAST of MC11g1320.1 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 2.5e-29
Identity = 57/103 (55.34%), Postives = 80/103 (77.67%), Query Frame = 0


Query: 61  LAPARADWKETSEGHVIRMDVPGLAKEELRIEVDDNRILKVMGERKREEEREGDHWHRVE 120
           +A AR DWKET+E HV + D+PG+ KEE+++E++D+ +LK+ GER  E+E + D WHRVE
Sbjct: 43  IANARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVE 102

Query: 121 RSYGKFWRQFRMPSDVDLDSVKATLENGVLTVTLPKLSPEKIK 164
           RS G F R+FR+P +V +D VKA++ENGVLTVT+PK+   K K
Sbjct: 103 RSSGGFSRKFRLPENVKMDQVKASMENGVLTVTVPKVETNKKK 145

The following BLAST results are available for this feature:

BLAST of MC11g1320.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30236	8.6e-51	56.83	22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1	[more]
Q38806	5.2e-48	60.40	22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1	[more]
P19244	1.2e-47	58.75	22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV...	[more]
Q7XUW5	1.4e-40	53.79	23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 ...	[more]
Q53M11	3.8e-30	48.30	21.9 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP21.9 ...	[more]

BLAST of MC11g1320.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156025.1	4.22e-125	99.46	22.0 kDa class IV heat shock protein [Momordica charantia]	[more]
ADU55793.1	1.52e-63	62.64	HSP20.3 [Citrullus lanatus]	[more]
XP_008459498.1	1.29e-62	60.64	PREDICTED: 22.0 kDa class IV heat shock protein [Cucumis melo]	[more]
KAA0039359.1	1.29e-62	60.64	22.0 kDa class IV heat shock protein [Cucumis melo var. makuwa] >TYK00542.1 22.0...	[more]
XP_042969442.1	3.35e-62	63.80	22.0 kDa class IV heat shock protein [Carya illinoinensis] >KAG6659123.1 hypothe...	[more]

BLAST of MC11g1320.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DP53	2.04e-125	99.46	22.0 kDa class IV heat shock protein OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
H6TB46	7.38e-64	62.64	HSP20.3 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A5A7TDL3	6.25e-63	60.64	22.0 kDa class IV heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3CAU1	6.25e-63	60.64	22.0 kDa class IV heat shock protein OS=Cucumis melo OX=3656 GN=LOC103498616 PE=...	[more]
I1LC12	4.35e-62	58.86	SHSP domain-containing protein OS=Glycine max OX=3847 GN=GLYMA_10G176400 PE=3 SV...	[more]

BLAST of MC11g1320.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10250.1	3.7e-49	60.40	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	1.5e-29	54.63	heat shock protein 18.2	[more]
AT1G53540.1	1.9e-29	48.23	HSP20-like chaperones superfamily protein	[more]
AT1G07400.1	2.5e-29	48.48	HSP20-like chaperones superfamily protein	[more]
AT1G59860.1	2.5e-29	55.34	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 67..170 e-value: 7.0E-31 score: 106.4
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 55..173 score: 28.145498
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 43..168 e-value: 4.6E-38 score: 132.3
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 61..164
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 163..185
None	No IPR available	PANTHER	PTHR11527:SF267	22.0 KDA HEAT SHOCK PROTEIN	coord: 29..174
None	No IPR available	CDD	cd06472	ACD_ScHsp26_like	coord: 65..156 e-value: 1.36639E-48 score: 151.303
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 29..174

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g1320	MC11g1320	gene

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1320.1-utr3	MC11g1320.1-utr3-MC11:16027857..16027973	utr3

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1320.1-cds	MC11g1320.1-cds-MC11:16027974..16028231	CDS
MC11g1320.1-cds	MC11g1320.1-cds-MC11:16028974..16029273	CDS

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1320.1-utr5	MC11g1320.1-utr5-MC11:16029274..16029282	utr5

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g1320.1	MC11g1320.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding