MC11g1289.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1289.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	CCHC-type domain-containing protein
Location	MC11: 15169595 .. 15169915 (-)
Sequence length	321
RNA-Seq Expression	MC11g1289.1
Synteny	MC11g1289.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTAAACAAATCCCAGATCTATCAGAATTATGTGCTTGGATTACCTCTTACGCAGAGAAAGCCAAGATGACCTCTTCCTCCTCATCTCTGAATCCTCTTCGTCGTTGTGGGTCAGATAACATTGTTGAATCTTGGAATCATCTTCCACATGGTTGGATCCTTCTTAATGTCGATGCCGCTTGCTGTGGAACTAATTCGAGATCGAGGATAGGAGCAATCCTTAGGGATTCGGCGGGGACAATTTTGGCAGCCCGTGCTTCGTCTGGCCCCAACTCCCTTAGCCCACTCCTTGCGGAAATTCATGCGATTTTGGAAGGTCTT

mRNA sequence

GGTAAACAAATCCCAGATCTATCAGAATTATGTGCTTGGATTACCTCTTACGCAGAGAAAGCCAAGATGACCTCTTCCTCCTCATCTCTGAATCCTCTTCGTCGTTGTGGGTCAGATAACATTGTTGAATCTTGGAATCATCTTCCACATGGTTGGATCCTTCTTAATGTCGATGCCGCTTGCTGTGGAACTAATTCGAGATCGAGGATAGGAGCAATCCTTAGGGATTCGGCGGGGACAATTTTGGCAGCCCGTGCTTCGTCTGGCCCCAACTCCCTTAGCCCACTCCTTGCGGAAATTCATGCGATTTTGGAAGGTCTT

Coding sequence (CDS)

GGTAAACAAATCCCAGATCTATCAGAATTATGTGCTTGGATTACCTCTTACGCAGAGAAAGCCAAGATGACCTCTTCCTCCTCATCTCTGAATCCTCTTCGTCGTTGTGGGTCAGATAACATTGTTGAATCTTGGAATCATCTTCCACATGGTTGGATCCTTCTTAATGTCGATGCCGCTTGCTGTGGAACTAATTCGAGATCGAGGATAGGAGCAATCCTTAGGGATTCGGCGGGGACAATTTTGGCAGCCCGTGCTTCGTCTGGCCCCAACTCCCTTAGCCCACTCCTTGCGGAAATTCATGCGATTTTGGAAGGTCTT

Protein sequence

GKQIPDLSELCAWITSYAEKAKMTSSSSSLNPLRRCGSDNIVESWNHLPHGWILLNVDAACCGTNSRSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHAILEGL

Homology

[Close]

BLAST of MC11g1289.1 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 60.5 bits (145), Expect = 6.58e-08
Identity = 38/107 (35.51%), Postives = 57/107 (53.27%), Query Frame = 0


Query: 1    GKQIPDLSELCAWITSYAEKAKMTSSSSSLNPLRRCGSDNIVESWNHLPHGWILLNVDAA 60
            GKQ+  +   C W+T + +       S + +P  +     +V+ W       + LN DAA
Sbjct: 1251 GKQVSPVEFKCEWLTPFLDSHSQAQMS-NYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAA 1310

Query: 61   CCGTNSRSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHAILEGL 107
            C G ++    G I+RDS+ +++AA +   P  LSPLLAEI  ILEGL
Sbjct: 1311 CRGASTS--FGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGL 1354

[Close]

BLAST of MC11g1289.1 vs. NCBI nr
Match: PPS12084.1 (hypothetical protein GOBAR_AA08553 [Gossypium barbadense])

HSP 1 Score: 55.8 bits (133), Expect = 1.26e-06
Identity = 34/105 (32.38%), Postives = 53/105 (50.48%), Query Frame = 0


Query: 5   PDLSELCAWITSYAEKAKMTSSSSSLNPLRRCGSDN--IVESWNHLPHGWILLNVDAACC 64
           PD+S +  W T Y ++ K      S N L+  G +   +   W   PHG+  LNVDA   
Sbjct: 76  PDISSVPNWRTEYIDRLK------SANQLQYTGFEQRLLAAKWKKPPHGFFKLNVDARRG 135

Query: 65  GTNSRSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHAILEGL 107
             +++   GA+LRD+ G+ +AAR+         LL E + +L G+
Sbjct: 136 SCSNKMGFGAVLRDANGSFIAARSWKQAGQADKLLMEANVVLLGI 174

[Close]

BLAST of MC11g1289.1 vs. NCBI nr
Match: ABD32401.2 (Ribonuclease H, partial [Medicago truncatula])

HSP 1 Score: 55.8 bits (133), Expect = 1.53e-06
Identity = 39/101 (38.61%), Postives = 54/101 (53.47%), Query Frame = 0


Query: 7   LSELCAWITSYAEKAKMTSSSSSLNPLRRCGSDNIVESWNHLPHGWILLNVDAACCGTNS 66
           L  LC  I S A+ A    +     P     SD +V+ WN+  H   +LNVD +C GT  
Sbjct: 35  LFRLCNNIISAADAANSAFNGEENPP----HSDRLVK-WNNRNHQDFILNVDGSCIGTPP 94

Query: 67  RSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHAILEGL 107
           R+  G +LR+SAG  ++  +   PNS   LLAE+ AI +GL
Sbjct: 95  RTGYGGVLRNSAGLFISGFSGLIPNSADILLAELTAIHQGL 130

[Close]

BLAST of MC11g1289.1 vs. NCBI nr
Match: KAF4363279.1 (hypothetical protein F8388_001820 [Cannabis sativa] >KAF4391434.1 hypothetical protein G4B88_005505 [Cannabis sativa])

HSP 1 Score: 53.9 bits (128), Expect = 1.21e-05
Identity = 29/65 (44.62%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 43  ESWNHLPHGWILLNVDAACCGTNSRSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHA 102
           E+W   P G++ LNVD A      ++ IGA++RDS G ++AARAS+    + P +AE  A
Sbjct: 423 ETWAPPPIGFLKLNVDGAISSQWGKTGIGALVRDSTGQVIAARASNRMGQMPPKVAEGWA 482

Query: 103 ILEGL 107
           +LEGL
Sbjct: 483 LLEGL 487

[Close]

BLAST of MC11g1289.1 vs. NCBI nr
Match: XP_024634748.1 (uncharacterized protein LOC112420238 [Medicago truncatula])

HSP 1 Score: 53.1 bits (126), Expect = 2.12e-05
Identity = 32/70 (45.71%), Postives = 44/70 (62.86%), Query Frame = 0


Query: 38  SDNIVESWNHLPHGWILLNVDAACCGTNSRSRIGAILRDSAGTILAARASSGPNSLSPLL 97
           SD  V+ WN+  H   +LNVD +C GT SR+  G ILR+SAG +++  +   PNS   L 
Sbjct: 177 SDRFVK-WNNRNHHDHILNVDGSCLGTPSRTGYGDILRNSAGLLISGFSGFIPNSTDILQ 236

Query: 98  AEIHAILEGL 107
           AE+ AI +GL
Sbjct: 237 AELTAIHQGL 245

[Close]

BLAST of MC11g1289.1 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 3.18e-08
Identity = 38/107 (35.51%), Postives = 57/107 (53.27%), Query Frame = 0


Query: 1    GKQIPDLSELCAWITSYAEKAKMTSSSSSLNPLRRCGSDNIVESWNHLPHGWILLNVDAA 60
            GKQ+  +   C W+T + +       S + +P  +     +V+ W       + LN DAA
Sbjct: 1251 GKQVSPVEFKCEWLTPFLDSHSQAQMS-NYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAA 1310

Query: 61   CCGTNSRSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHAILEGL 107
            C G ++    G I+RDS+ +++AA +   P  LSPLLAEI  ILEGL
Sbjct: 1311 CRGASTS--FGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGL 1354

[Close]

BLAST of MC11g1289.1 vs. ExPASy TrEMBL
Match: A0A2P5Y918 (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA08553 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.12e-07
Identity = 34/105 (32.38%), Postives = 53/105 (50.48%), Query Frame = 0


Query: 5   PDLSELCAWITSYAEKAKMTSSSSSLNPLRRCGSDN--IVESWNHLPHGWILLNVDAACC 64
           PD+S +  W T Y ++ K      S N L+  G +   +   W   PHG+  LNVDA   
Sbjct: 76  PDISSVPNWRTEYIDRLK------SANQLQYTGFEQRLLAAKWKKPPHGFFKLNVDARRG 135

Query: 65  GTNSRSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHAILEGL 107
             +++   GA+LRD+ G+ +AAR+         LL E + +L G+
Sbjct: 136 SCSNKMGFGAVLRDANGSFIAARSWKQAGQADKLLMEANVVLLGI 174

[Close]

BLAST of MC11g1289.1 vs. ExPASy TrEMBL
Match: Q2HTL5 (Ribonuclease H (Fragment) OS=Medicago truncatula OX=3880 GN=MtrDRAFT_AC150244g34v2 PE=4 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 7.41e-07
Identity = 39/101 (38.61%), Postives = 54/101 (53.47%), Query Frame = 0


Query: 7   LSELCAWITSYAEKAKMTSSSSSLNPLRRCGSDNIVESWNHLPHGWILLNVDAACCGTNS 66
           L  LC  I S A+ A    +     P     SD +V+ WN+  H   +LNVD +C GT  
Sbjct: 35  LFRLCNNIISAADAANSAFNGEENPP----HSDRLVK-WNNRNHQDFILNVDGSCIGTPP 94

Query: 67  RSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHAILEGL 107
           R+  G +LR+SAG  ++  +   PNS   LLAE+ AI +GL
Sbjct: 95  RTGYGGVLRNSAGLFISGFSGLIPNSADILLAELTAIHQGL 130

[Close]

BLAST of MC11g1289.1 vs. ExPASy TrEMBL
Match: A0A1J3C7A8 (Putative ribonuclease H protein (Fragment) OS=Noccaea caerulescens OX=107243 GN=GA_TR12511_c0_g1_i1_g.40014 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.40e-06
Identity = 33/91 (36.26%), Postives = 45/91 (49.45%), Query Frame = 0


Query: 13  WITSYAEKAKMTSSSSSLNPLRRCGSDNIVESWNHLPHGWILLNVDAACCGTNSRSRIGA 72
           W+ S  EK+K +  SS  N       D+ + +W   P GW+  NVD      + RS IG 
Sbjct: 562 WVKSTEEKSKKSKDSSKPN-------DSSINAWKPPPQGWLKCNVDGTWSEEHDRSGIGW 621

Query: 73  ILRDSAGTILAARASSGPNSLSPLLAEIHAI 103
           +LRDS G ++   A    N  SPL  EI A+
Sbjct: 622 VLRDSRGKVIWMGARKTKNLGSPLATEIEAL 645

[Close]

BLAST of MC11g1289.1 vs. ExPASy TrEMBL
Match: A0A7J6F041 (CCHC-type domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_001820 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 5.87e-06
Identity = 29/65 (44.62%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 43  ESWNHLPHGWILLNVDAACCGTNSRSRIGAILRDSAGTILAARASSGPNSLSPLLAEIHA 102
           E+W   P G++ LNVD A      ++ IGA++RDS G ++AARAS+    + P +AE  A
Sbjct: 423 ETWAPPPIGFLKLNVDGAISSQWGKTGIGALVRDSTGQVIAARASNRMGQMPPKVAEGWA 482

Query: 103 ILEGL 107
           +LEGL
Sbjct: 483 LLEGL 487

The following BLAST results are available for this feature:

BLAST of MC11g1289.1 vs.


Match Name	E-value	Identity	Description

BLAST of MC11g1289.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158377.1	6.58e-08	35.51	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
PPS12084.1	1.26e-06	32.38	hypothetical protein GOBAR_AA08553 [Gossypium barbadense]	[more]
ABD32401.2	1.53e-06	38.61	Ribonuclease H, partial [Medicago truncatula]	[more]
KAF4363279.1	1.21e-05	44.62	hypothetical protein F8388_001820 [Cannabis sativa] >KAF4391434.1 hypothetical p...	[more]
XP_024634748.1	2.12e-05	45.71	uncharacterized protein LOC112420238 [Medicago truncatula]	[more]

BLAST of MC11g1289.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DX30	3.18e-08	35.51	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A2P5Y918	6.12e-07	32.38	Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA08553 PE=4 SV...	[more]
Q2HTL5	7.41e-07	38.61	Ribonuclease H (Fragment) OS=Medicago truncatula OX=3880 GN=MtrDRAFT_AC150244g34...	[more]
A0A1J3C7A8	2.40e-06	36.26	Putative ribonuclease H protein (Fragment) OS=Noccaea caerulescens OX=107243 GN=...	[more]
A0A7J6F041	5.87e-06	44.62	CCHC-type domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_001820 P...	[more]

BLAST of MC11g1289.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 56..107 e-value: 1.6E-6 score: 28.0
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 55..107 e-value: 6.91907E-10 score: 50.3904
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 50..107

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g1289	MC11g1289	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1289.1-cds	MC11g1289.1-cds-MC11:15169595..15169915	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g1289.1	MC11g1289.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity