MC10g1161.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC10g1161.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionrRNA 2'-O-methyltransferase fibrillarin
LocationMC10: 14666183 .. 14669648 (+)
Sequence length1169
RNA-Seq ExpressionMC10g1161.1
SyntenyMC10g1161.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCCGCGGTGGTGGTGGTGGATTTAGGGGCAGGGGTGATGGAGGAAGGGGCGGTAGAGGCAGAGGTGGTGGAGGTAGAGGTGGTGGTGGAGGCAGAGGTATGCAGAGCCGTGGTGGTGGTCGAGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGCGGTGGAATGAAAGGTGGAAGCAAAGTTGTGATTGAACCCCACAGACATGAAGGTATTTTCATAGCCAAGGGCAAAGAAGATGCTCTTGTCACTAAGAATATGGTCTCTGGTGAATCTGTCTACAATGAGAAGAGGATATCCGTACAGGTAATCTCACCTTGAAATAATGCTTGCGCCTGGATGTTTGTTATCTCTCATAGTGAATTTTTGAAATTTAGTGACACCATTCTATACGAAATATTGACTATGTGAGTGCTTGATTATTACTCATGAGAAATTCTAGTTGGAATTAATATCTCCAAATAGTTTTGTGTGGTTCTGGCACAATAGTTAGGGAGTGTATATGAAAGTACTTGGCTTTATGCGGGTGCTGTGTTTGTAAGCTTATAAGCTAAATGTTTTATATATACTGTCCTTCCAGAATGAAGATGGAACTAAAGTTGAATACAGAGTTTGGAACCCATTCAGGTCAAAGTTGGCGGCTGCAGTTCTTGGTGGCGTTGATGACATATGGATTGTTAGTGCTTTTCACCTAAAATTTTGATTTTATCTACTTCAATTTTTCAGTTTGTTATTTTCTTTGCTTCTTTATTTTTAATTTGCTAACATGTGTATTGTAGAAACCTGGTGCTCGAGTGTTGTATCTTGGCGCTGCTTCAGGAACAACAGTATCTCATGTATCAGATCTTGTGGGGCCTGTAAGTAGTTATTTATAGAGTATATGTAGTAGTCTTTCATTGAAGTAAAAAATGCTTAACCACTGTCGTTCTGATTTGCAGACTGGTATAGTGTATGCTGTGGAATTCTCTCATAGAAGTGGTAGAGATTTGGTGAACATGGCAAAGAAGCGCACAAATGTTATACCCATCATTGAAGATGCTAGACACCCTGCCAAGTATCGAATGTTGGTTGGCATGGTGGATGTGGTCTTTTCAGATGTTGCTCAGCCAGATCAGGTTCACACTTTGTTTTTTACTAGTCTTATTATTATTGTATGTTAACGTCATCACCTTTGTTGAGATTGATGGCACACTTCTTCGACTTGTGTGATTAATAGAATCCTACCATTGATTTTTGTATCTTATTGATTAATCTTTTTAAGTGGTGCAGTACATTTCAAACTATAGTTTTCATTTGTACAGTCTTGTTGGATTTAATGCCTACTTATCTCTCCATGTTTCTAGCTTAAAGCCTTTTTTATCCTCAAATTATTTAGCATATGATGAATTGCTCGGATTCCCCTTCAAGACTTCTTGAATGATGCAAGTTTCGAGTTTCTGATGCTTCACAAAGACTTACTTTGTCTTAGTTTTTTCATAATTGCTATCTAGCAGACTGTTTATTTTCATACTTTTTCTCCTTGAACCATATAGCATATGACGAATTTCTCGAATTTTCCTTCTTGTATGATGCAAGTCCTGACTTTCAGATCCTTCAAAAAGACTTGATTTTTACTGTTTCTTTTCATACATTTTCTCCTTGAACTATTTGGCATATGATGAATTTCTCAGATTCCATTCAAGACTTCTTGAATGGTGTAAGTCCTGAGTTTCAGATGCTTCAAAAAGACTTGCTTTTGAATTGTCGTCATTGTTTCTTAATAAATGCTTATCAATTCTGGTGATACTATCCGGATTCTTCTTTTCAAAATTTTCCTGGGTCTGTTTTCCTTTCTCCCATGTTAACATCCTATTCCATCTCAAGTAGATTGTATAAAATCAATCCAAGCCTTATTATTTTTGTCTAGGTACCCCTTACCCTGTGCTTAAAGTTCATAAGTTGCATTTCTTCATTTTTTATATTCATGAAATACTCTATGTAAACAATTGCTTTTTTTAGAACTTTGGATCAAACCATCTTGTTCTCTCAAAGCATTAGTACTAATCAACTGATTCTATATTTTTTATTCCAATACGTGAAATAATTCCACTATCACCTTTACATTCCATATTTACACTGCCCACATTTTCCATCACACCTCTTTTTGTGTATAGTAGAAGATTTTCATCTTTTATTAGCACTGGAAATTAGAAGTCTTTTTTCTGTTTAGCTGTATTTTTTTTGAGTTGGATGATTATTCTAATTGCCAACCATTTCTGGATCATTCAATATGTGGGGTCTTTGTGTAAAAATCTCTCTAATTGTTCTCCTATTAAAAGAACCCTTAATTTGAAATTCAGCATATGCTTTATTCATTGTGACCAATGATCTGTTCTGGAATCTGGATCTTATAATACAGAAAGTTAAATTTTGGAGGTTTAGAATTTTTCTGTAGTCGATTATAGAGCTCAACTTCAAGCAAGAAGATGAAATTATAAAAGAAAGCATCGGCTATCTTATTTAGTTCTGGGTTATGTGCGGGATAATGAGATTGTGTCAGAAATTTAATAATCTGAAGGTCTCTCACCAATTTCAATTAAGATAATTTTATCACTTATTAATTTGATTTGATGTCGTGCAGGTAAATTCTCACGTTTTTCATTTTAGGCTCTTCCTATTTGCTAAGCATGTGATGAATTACTCGGATTCCCCTTCAAGACTTCTTGAATGATGCATAACCCGAGTTTGTGATGCGTCAAGAATATCACATTTTGATAAAACCCTTCATTTGCTACCAATTTTGCATTCTGGCTTGGCTTATTTTTTGAAATCATGAACTTCTCGGTCTTTCATCTTCTAAAGTTGACCACTTTTGACTTGTTCAATGCAGGCTAGAATTTTGGCACTTAATGCATCATATTTTCTCAAGGCGGGTGGCCATTTTGTTATATCAATCAAAGTGAGTTCAAGATTTGCATTTTGTTAAAGACAATAGACAGTATAATCCATGAATATAAGATAATATGGTATTGAACCTTTTTCAGGCCAACTGCATCGACTCGACCGTTCCAGCAGAGGCAGTGTTTGCAAGTGAGGTGAATAAGCTAAAGGCAGATCAATTCAAACCCACTGAGCAAGTAACTCTTGAACCATTTGAGCGAGATCATGCTTGTGTTGTTGGTATCTATCGGGCGCCGAAGAAACAGAAGCCCGCTGCATAGACTATCCAATGAACTAGGATCTTGATTATGTGAACAGTTTATTGATTGCTACTGCTAGCTTGTAGGCGGGATCATTTTAAAGGGGAGACTATGTTTTTGATATCTTTGGTGATTAATGGAGGACATAAATATGTTGTTTTTTTTTTCACTACTTTGTTTTGTCTCTTTTGAAGTATAAGAATTGATAAATGGTAGAGAGATATATGTTTAGTTTTCTTTCTTATTTGACAGAAGAAATGAATGTGCACTTGCTATGTCTTTTTATAAAC

mRNA sequence

GGCCGCGGTGGTGGTGGTGGATTTAGGGGCAGGGGTGATGGAGGAAGGGGCGGTAGAGGCAGAGGTGGTGGAGGTAGAGGTGGTGGTGGAGGCAGAGGTATGCAGAGCCGTGGTGGTGGTCGAGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGCGGTGGAATGAAAGGTGGAAGCAAAGTTGTGATTGAACCCCACAGACATGAAGGTATTTTCATAGCCAAGGGCAAAGAAGATGCTCTTGTCACTAAGAATATGGTCTCTGGTGAATCTGTCTACAATGAGAAGAGGATATCCGTACAGAATGAAGATGGAACTAAAGTTGAATACAGAGTTTGGAACCCATTCAGGTCAAAGTTGGCGGCTGCAGTTCTTGGTGGCGTTGATGACATATGGATTAAACCTGGTGCTCGAGTGTTGTATCTTGGCGCTGCTTCAGGAACAACAGTATCTCATGTATCAGATCTTGTGGGGCCTACTGGTATAGTGTATGCTGTGGAATTCTCTCATAGAAGTGGTAGAGATTTGGTGAACATGGCAAAGAAGCGCACAAATGTTATACCCATCATTGAAGATGCTAGACACCCTGCCAAGTATCGAATGTTGGTTGGCATGGTGGATGTGGTCTTTTCAGATGTTGCTCAGCCAGATCAGGCTAGAATTTTGGCACTTAATGCATCATATTTTCTCAAGGCGGGTGGCCATTTTGTTATATCAATCAAAGCCAACTGCATCGACTCGACCGTTCCAGCAGAGGCAGTGTTTGCAAGTGAGGTGAATAAGCTAAAGGCAGATCAATTCAAACCCACTGAGCAAGTAACTCTTGAACCATTTGAGCGAGATCATGCTTGTGTTGTTGGTATCTATCGGGCGCCGAAGAAACAGAAGCCCGCTGCATAGACTATCCAATGAACTAGGATCTTGATTATGTGAACAGTTTATTGATTGCTACTGCTAGCTTGTAGGCGGGATCATTTTAAAGGGGAGACTATGTTTTTGATATCTTTGGTGATTAATGGAGGACATAAATATGTTGTTTTTTTTTTCACTACTTTGTTTTGTCTCTTTTGAAGTATAAGAATTGATAAATGGTAGAGAGATATATGTTTAGTTTTCTTTCTTATTTGACAGAAGAAATGAATGTGCACTTGCTATGTCTTTTTATAAAC

Coding sequence (CDS)

GGCCGCGGTGGTGGTGGTGGATTTAGGGGCAGGGGTGATGGAGGAAGGGGCGGTAGAGGCAGAGGTGGTGGAGGTAGAGGTGGTGGTGGAGGCAGAGGTATGCAGAGCCGTGGTGGTGGTCGAGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGCGGTGGAATGAAAGGTGGAAGCAAAGTTGTGATTGAACCCCACAGACATGAAGGTATTTTCATAGCCAAGGGCAAAGAAGATGCTCTTGTCACTAAGAATATGGTCTCTGGTGAATCTGTCTACAATGAGAAGAGGATATCCGTACAGAATGAAGATGGAACTAAAGTTGAATACAGAGTTTGGAACCCATTCAGGTCAAAGTTGGCGGCTGCAGTTCTTGGTGGCGTTGATGACATATGGATTAAACCTGGTGCTCGAGTGTTGTATCTTGGCGCTGCTTCAGGAACAACAGTATCTCATGTATCAGATCTTGTGGGGCCTACTGGTATAGTGTATGCTGTGGAATTCTCTCATAGAAGTGGTAGAGATTTGGTGAACATGGCAAAGAAGCGCACAAATGTTATACCCATCATTGAAGATGCTAGACACCCTGCCAAGTATCGAATGTTGGTTGGCATGGTGGATGTGGTCTTTTCAGATGTTGCTCAGCCAGATCAGGCTAGAATTTTGGCACTTAATGCATCATATTTTCTCAAGGCGGGTGGCCATTTTGTTATATCAATCAAAGCCAACTGCATCGACTCGACCGTTCCAGCAGAGGCAGTGTTTGCAAGTGAGGTGAATAAGCTAAAGGCAGATCAATTCAAACCCACTGAGCAAGTAACTCTTGAACCATTTGAGCGAGATCATGCTTGTGTTGTTGGTATCTATCGGGCGCCGAAGAAACAGAAGCCCGCTGCATAG

Protein sequence

GRGGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Homology
BLAST of MC10g1161.1 vs. ExPASy Swiss-Prot
Match: Q94AH9 (rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB2 PE=1 SV=2)

HSP 1 Score: 446.4 bits (1147), Expect = 2.5e-124
Identity = 256/306 (83.66%), Postives = 269/306 (87.91%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGG-GRGGGRGRGGGRG------- 60
           GRG GG   GRGDGG  G GRGGGGR  GGGRG   RGG GRG G  RGG RG       
Sbjct: 15  GRGRGGYSGGRGDGGFSG-GRGGGGR--GGGRGFSDRGGRGRGRGPPRGGARGGRGPAGR 74

Query: 61  GGMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRV 120
           GGMKGGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTK EYRV
Sbjct: 75  GGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKTEYRV 134

Query: 121 WNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRS 180
           WNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSDLVGP G VYAVEFSHRS
Sbjct: 135 WNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRS 194

Query: 181 GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKA 240
           GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLK+
Sbjct: 195 GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKS 254

Query: 241 GGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAP 299
           GGHFVISIKANCIDSTVPAEAVF +EV KL+ +QFKP EQVTLEPFERDHACVVG YR P
Sbjct: 255 GGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMP 314

BLAST of MC10g1161.1 vs. ExPASy Swiss-Prot
Match: Q9FEF8 (rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB1 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.3e-122
Identity = 252/303 (83.17%), Postives = 265/303 (87.46%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGG----GRGGGRGRGGGRGGGMK 60
           G  GGGGFRG  DG  GGRG GGG   GGG  G + R G    GRG  RGRGG   GGMK
Sbjct: 7   GGRGGGGFRGGRDG--GGRGFGGGRSFGGGRSGDRGRSGPRGRGRGAPRGRGGPPRGGMK 66

Query: 61  GGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPF 120
           GGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTKVEYRVWNPF
Sbjct: 67  GGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYRVWNPF 126

Query: 121 RSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDL 180
           RSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSDLVGP G VYAVEFSHRSGRDL
Sbjct: 127 RSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRDL 186

Query: 181 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHF 240
           VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNAS+FLK GGHF
Sbjct: 187 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASFFLKTGGHF 246

Query: 241 VISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK 300
           VISIKANCIDSTV AEAVF SEV KL+ +QFKP EQVTLEPFERDHACVVG YR PKKQK
Sbjct: 247 VISIKANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKQK 306

BLAST of MC10g1161.1 vs. ExPASy Swiss-Prot
Match: Q8I1F4 (rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila erecta OX=7220 GN=Fib PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 4.3e-108
Identity = 226/297 (76.09%), Postives = 252/297 (84.85%), Query Frame = 0

Query: 1   GRGG-GGGFRGRGDGGRGGRGRGG-GGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGG 60
           GRGG GGG  G G GG GG GRGG GGRGGGGGRG   RGGG  GG GRGGG  GG KGG
Sbjct: 51  GRGGFGGGRGGGGRGGGGGGGRGGFGGRGGGGGRGGGGRGGGGRGGGGRGGG-AGGFKGG 110

Query: 61  SKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRS 120
             V IEPHRHEG+FIA+GKEDALVT+N V G  VY EKRISV+N +G K+EYRVWNPFRS
Sbjct: 111 KTVTIEPHRHEGVFIARGKEDALVTRNFVPGSEVYGEKRISVEN-NGEKIEYRVWNPFRS 170

Query: 121 KLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVN 180
           KLAAAVLGGV+ I + PG++VLYLGAASGTTVSHVSD+VGP G+VYAVEFSHRSGRDL+N
Sbjct: 171 KLAAAVLGGVEQIHMPPGSKVLYLGAASGTTVSHVSDVVGPEGLVYAVEFSHRSGRDLIN 230

Query: 181 MAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVI 240
           +AKKRTN+IPIIEDARHP KYRMLVGMVD +F+DVAQPDQ RI+ALNA +FLK GGHFVI
Sbjct: 231 VAKKRTNIIPIIEDARHPHKYRMLVGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHFVI 290

Query: 241 SIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQ 296
           SIKA+CIDST   EAVFASEV K++AD+ KP EQ+TLEP+ERDHA VVG+YR P KQ
Sbjct: 291 SIKASCIDSTAQPEAVFASEVKKMQADKLKPQEQLTLEPYERDHAVVVGVYRPPPKQ 345

BLAST of MC10g1161.1 vs. ExPASy Swiss-Prot
Match: Q9W1V3 (rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila melanogaster OX=7227 GN=Fib PE=2 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.6e-107
Identity = 224/299 (74.92%), Postives = 251/299 (83.95%), Query Frame = 0

Query: 2   RGGGGGF-RGRGDGGRGGRGRGG----GGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMK 61
           RGG GGF  GRG GGRGG G GG    GGRGGGGGRG   RGGG  GG GRGGG  GG K
Sbjct: 48  RGGRGGFGGGRGGGGRGGGGGGGRGAFGGRGGGGGRGGGGRGGGGRGGGGRGGG-AGGFK 107

Query: 62  GGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPF 121
           GG  V IEPHRHEG+FIA+GKEDALVT+N V G  VY EKRISV+  +G K+EYRVWNPF
Sbjct: 108 GGKTVTIEPHRHEGVFIARGKEDALVTRNFVPGSEVYGEKRISVET-NGEKIEYRVWNPF 167

Query: 122 RSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDL 181
           RSKLAAAVLGGV+ I + PG++VLYLGAASGTTVSHVSD+VGP G+VYAVEFSHRSGRDL
Sbjct: 168 RSKLAAAVLGGVEQIHMPPGSKVLYLGAASGTTVSHVSDVVGPEGLVYAVEFSHRSGRDL 227

Query: 182 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHF 241
           +N+AKKRTN+IPIIEDARHP KYRMLVGMVD +F+DVAQPDQ RI+ALNA +FLK GGHF
Sbjct: 228 INVAKKRTNIIPIIEDARHPHKYRMLVGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHF 287

Query: 242 VISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQ 296
           VISIKA+CIDST   EAVFA+EV K++AD+ KP EQ+TLEP+ERDHA VVG+YR P KQ
Sbjct: 288 VISIKASCIDSTAQPEAVFAAEVKKMQADKLKPQEQLTLEPYERDHAVVVGVYRPPPKQ 344

BLAST of MC10g1161.1 vs. ExPASy Swiss-Prot
Match: P22509 (rRNA 2'-O-methyltransferase fibrillarin OS=Rattus norvegicus OX=10116 GN=Fbl PE=1 SV=2)

HSP 1 Score: 387.9 bits (995), Expect = 1.0e-106
Identity = 222/306 (72.55%), Postives = 252/306 (82.35%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGGG--------RGGGRGRGGGRG 60
           GRGGG G RG   GGRGG G GG GRGGGGG G + RGGG         GGGRGRGGGRG
Sbjct: 23  GRGGGRGGRGGFGGGRGGFGGGGRGRGGGGG-GFRGRGGGGGRGGGFQSGGGRGRGGGRG 82

Query: 61  G--GMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEY 120
           G  G + G  V++EPHRHEG+FI +GKEDALVTKN+V GESVY EKR+S+ +E   K+EY
Sbjct: 83  GKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSI-SEGDDKIEY 142

Query: 121 RVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSH 180
           R WNPFRSKLAAA+LGGVD I IKPGA+VLYLGAASGTTVSHVSD+VGP G+VYAVEFSH
Sbjct: 143 RAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSH 202

Query: 181 RSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFL 240
           RSGRDL+N+AKKRTN+IP+IEDARHP KYRML+ MVDV+F+DVAQPDQ RI+ALNA  FL
Sbjct: 203 RSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFL 262

Query: 241 KAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYR 297
           + GGHFVISIKANCIDST  AEAVFASEV K++ +  KP EQ+TLEP+ERDHA VVG+YR
Sbjct: 263 RNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYR 322

BLAST of MC10g1161.1 vs. NCBI nr
Match: XP_022141240.1 (mediator of RNA polymerase II transcription subunit 36a-like [Momordica charantia])

HSP 1 Score: 538 bits (1386), Expect = 8.67e-192
Identity = 296/300 (98.67%), Postives = 298/300 (99.33%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RGGGGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

BLAST of MC10g1161.1 vs. NCBI nr
Match: XP_008439295.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 36a-like [Cucumis melo])

HSP 1 Score: 535 bits (1379), Expect = 1.05e-190
Identity = 293/300 (97.67%), Postives = 298/300 (99.33%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVV+EPHRHEGIFIAKGKEDALVTKNMV+GESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVTGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

BLAST of MC10g1161.1 vs. NCBI nr
Match: XP_023552409.1 (mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535 bits (1377), Expect = 2.04e-190
Identity = 294/300 (98.00%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RGGGGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

BLAST of MC10g1161.1 vs. NCBI nr
Match: XP_038875959.1 (rRNA 2'-O-methyltransferase fibrillarin 1-like [Benincasa hispida])

HSP 1 Score: 533 bits (1373), Expect = 8.29e-190
Identity = 290/297 (97.64%), Postives = 294/297 (98.99%), Query Frame = 0

Query: 3   GGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGSKVV 62
           GGGGGFRGRGDGGRGGRGRGGGG  GGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGSKVV
Sbjct: 9   GGGGGFRGRGDGGRGGRGRGGGGGRGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGSKVV 68

Query: 63  IEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSKLAA 122
           +EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFRSKLAA
Sbjct: 69  VEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSKLAA 128

Query: 123 AVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNMAKK 182
           AVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGPTGIVYAVEFSHRSGRDLVNMAKK
Sbjct: 129 AVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNMAKK 188

Query: 183 RTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVISIKA 242
           RTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVISIKA
Sbjct: 189 RTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISIKA 248

Query: 243 NCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           NCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 249 NCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

BLAST of MC10g1161.1 vs. NCBI nr
Match: XP_023542882.1 (mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 533 bits (1372), Expect = 1.27e-189
Identity = 292/302 (96.69%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGGRGG---GGGRGMQSRGGGRGGGRGRGGGRGGGMKG 60
           GRGGGGGFRGRGDGGRGGRGRGGGG GG   GGGRGM SRGGGRGGGRGRGGGRGGGMKG
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGSGGRGFGGGRGMASRGGGRGGGRGRGGGRGGGMKG 65

Query: 61  GSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFR 120
           GSKVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFR
Sbjct: 66  GSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFR 125

Query: 121 SKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLV 180
           SKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGP+GIVYAVEFSHRSGRDLV
Sbjct: 126 SKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSGRDLV 185

Query: 181 NMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFV 240
           NMAKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFV
Sbjct: 186 NMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFV 245

Query: 241 ISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKP 299
           ISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKP
Sbjct: 246 ISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKP 305

BLAST of MC10g1161.1 vs. ExPASy TrEMBL
Match: A0A6J1CHH3 (mediator of RNA polymerase II transcription subunit 36a-like OS=Momordica charantia OX=3673 GN=LOC111011688 PE=3 SV=1)

HSP 1 Score: 538 bits (1386), Expect = 4.20e-192
Identity = 296/300 (98.67%), Postives = 298/300 (99.33%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RGGGGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

BLAST of MC10g1161.1 vs. ExPASy TrEMBL
Match: A0A1S3AY25 (mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo OX=3656 GN=LOC103484122 PE=3 SV=1)

HSP 1 Score: 535 bits (1379), Expect = 5.07e-191
Identity = 293/300 (97.67%), Postives = 298/300 (99.33%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVV+EPHRHEGIFIAKGKEDALVTKNMV+GESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVTGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

BLAST of MC10g1161.1 vs. ExPASy TrEMBL
Match: A0A6J1HX74 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita maxima OX=3661 GN=LOC111467690 PE=3 SV=1)

HSP 1 Score: 532 bits (1371), Expect = 8.10e-190
Identity = 292/300 (97.33%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RG GGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGP+GIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

BLAST of MC10g1161.1 vs. ExPASy TrEMBL
Match: A0A6J1G280 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita moschata OX=3662 GN=LOC111449984 PE=3 SV=1)

HSP 1 Score: 532 bits (1371), Expect = 8.10e-190
Identity = 292/300 (97.33%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RG GGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGP+GIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

BLAST of MC10g1161.1 vs. ExPASy TrEMBL
Match: A0A6J1JA27 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita maxima OX=3661 GN=LOC111483086 PE=3 SV=1)

HSP 1 Score: 530 bits (1365), Expect = 6.41e-189
Identity = 294/300 (98.00%), Postives = 297/300 (99.00%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGG-RGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 60
           GRGGGGGFRGRGDGGRGGRGRGGGG RGGGGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGM-SRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 61  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 120
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 121 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNM 180
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSD+VGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 181 AKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVIS 240
           AKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 241 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 299
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 304

BLAST of MC10g1161.1 vs. TAIR 10
Match: AT4G25630.1 (fibrillarin 2 )

HSP 1 Score: 446.4 bits (1147), Expect = 1.8e-125
Identity = 256/306 (83.66%), Postives = 269/306 (87.91%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGG-GRGGGRGRGGGRG------- 60
           GRG GG   GRGDGG  G GRGGGGR  GGGRG   RGG GRG G  RGG RG       
Sbjct: 15  GRGRGGYSGGRGDGGFSG-GRGGGGR--GGGRGFSDRGGRGRGRGPPRGGARGGRGPAGR 74

Query: 61  GGMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRV 120
           GGMKGGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTK EYRV
Sbjct: 75  GGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKTEYRV 134

Query: 121 WNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRS 180
           WNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSDLVGP G VYAVEFSHRS
Sbjct: 135 WNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRS 194

Query: 181 GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKA 240
           GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNASYFLK+
Sbjct: 195 GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKS 254

Query: 241 GGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAP 299
           GGHFVISIKANCIDSTVPAEAVF +EV KL+ +QFKP EQVTLEPFERDHACVVG YR P
Sbjct: 255 GGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMP 314

BLAST of MC10g1161.1 vs. TAIR 10
Match: AT5G52470.1 (fibrillarin 1 )

HSP 1 Score: 439.9 bits (1130), Expect = 1.7e-123
Identity = 252/303 (83.17%), Postives = 265/303 (87.46%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGG----GRGGGRGRGGGRGGGMK 60
           G  GGGGFRG  DG  GGRG GGG   GGG  G + R G    GRG  RGRGG   GGMK
Sbjct: 7   GGRGGGGFRGGRDG--GGRGFGGGRSFGGGRSGDRGRSGPRGRGRGAPRGRGGPPRGGMK 66

Query: 61  GGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPF 120
           GGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTKVEYRVWNPF
Sbjct: 67  GGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYRVWNPF 126

Query: 121 RSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDL 180
           RSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSDLVGP G VYAVEFSHRSGRDL
Sbjct: 127 RSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRDL 186

Query: 181 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHF 240
           VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQARILALNAS+FLK GGHF
Sbjct: 187 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASFFLKTGGHF 246

Query: 241 VISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK 300
           VISIKANCIDSTV AEAVF SEV KL+ +QFKP EQVTLEPFERDHACVVG YR PKKQK
Sbjct: 247 VISIKANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKQK 306

BLAST of MC10g1161.1 vs. TAIR 10
Match: AT5G52490.1 (Fibrillarin family protein )

HSP 1 Score: 338.6 bits (867), Expect = 5.2e-93
Identity = 195/293 (66.55%), Postives = 226/293 (77.13%), Query Frame = 0

Query: 9   RGRGDGGRGGRGRGGGGRGGGGGRGMQSRGGGRGG--GRGRGGGR-----GGGMKGGSKV 68
           RGRG G RGGRG   GG G         RG GRGG  GRGRG GR      GG+KGGSKV
Sbjct: 6   RGRGGGVRGGRGLARGGEGSA------VRGSGRGGESGRGRGPGRVKSESDGGIKGGSKV 65

Query: 69  VIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSKLA 128
           ++ PHRH G+F+AK K DALVTKN+V GE +YNEKRI VQNED + VEYRVWNP RSKLA
Sbjct: 66  LVTPHRHAGVFVAKSKADALVTKNLVPGEIIYNEKRIFVQNEDRSTVEYRVWNPHRSKLA 125

Query: 129 AAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDLVNMAK 188
            A+  GVD+IWIKPG +VLYLGA+SG TVSHVSD+VGP G VYAVE S   G+ L+NMA+
Sbjct: 126 DAITTGVDNIWIKPGVKVLYLGASSGYTVSHVSDIVGPEGCVYAVEHSDICGKVLMNMAE 185

Query: 189 KRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQARILALNASYFLKAGGHFVISIK 248
           KRTNVIPIIEDARHPAKYRMLVGMVD++FSDV  P+QA IL+LNASYFLK+GGHF+ISIK
Sbjct: 186 KRTNVIPIIEDARHPAKYRMLVGMVDIIFSDVNHPEQANILSLNASYFLKSGGHFMISIK 245

Query: 249 ANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKK 295
           AN IDST+ AE V+  EV KL+ ++ +PTE + L+  E  HACV G YR P+K
Sbjct: 246 ANSIDSTIAAETVYQMEVEKLQMEELRPTEILHLDSCEEKHACVFGGYRLPRK 292

BLAST of MC10g1161.1 vs. TAIR 10
Match: AT5G52470.2 (fibrillarin 1 )

HSP 1 Score: 308.5 bits (789), Expect = 5.7e-84
Identity = 184/222 (82.88%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 1   GRGGGGGFRGRGDGGRGGRGRGGGGRGGGGGRGMQSRGG----GRGGGRGRGGGRGGGMK 60
           G  GGGGFRG  DG  GGRG GGG   GGG  G + R G    GRG  RGRGG   GGMK
Sbjct: 7   GGRGGGGFRGGRDG--GGRGFGGGRSFGGGRSGDRGRSGPRGRGRGAPRGRGGPPRGGMK 66

Query: 61  GGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPF 120
           GGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTKVEYRVWNPF
Sbjct: 67  GGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYRVWNPF 126

Query: 121 RSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDLVGPTGIVYAVEFSHRSGRDL 180
           RSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSDLVGP G VYAVEFSHRSGRDL
Sbjct: 127 RSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRDL 186

Query: 181 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVVFSDVAQPDQ 219
           VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDV+FSDVAQPDQ
Sbjct: 187 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQ 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AH92.5e-12483.66rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB... [more]
Q9FEF82.3e-12283.17rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB... [more]
Q8I1F44.3e-10876.09rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila erecta OX=7220 GN=Fib PE=3... [more]
Q9W1V31.6e-10774.92rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila melanogaster OX=7227 GN=Fi... [more]
P225091.0e-10672.55rRNA 2'-O-methyltransferase fibrillarin OS=Rattus norvegicus OX=10116 GN=Fbl PE=... [more]
Match NameE-valueIdentityDescription
XP_022141240.18.67e-19298.67mediator of RNA polymerase II transcription subunit 36a-like [Momordica charanti... [more]
XP_008439295.11.05e-19097.67PREDICTED: mediator of RNA polymerase II transcription subunit 36a-like [Cucumis... [more]
XP_023552409.12.04e-19098.00mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo sub... [more]
XP_038875959.18.29e-19097.64rRNA 2'-O-methyltransferase fibrillarin 1-like [Benincasa hispida][more]
XP_023542882.11.27e-18996.69mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo sub... [more]
Match NameE-valueIdentityDescription
A0A6J1CHH34.20e-19298.67mediator of RNA polymerase II transcription subunit 36a-like OS=Momordica charan... [more]
A0A1S3AY255.07e-19197.67mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo OX=... [more]
A0A6J1HX748.10e-19097.33probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita ma... [more]
A0A6J1G2808.10e-19097.33probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita mo... [more]
A0A6J1JA276.41e-18998.00probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita ma... [more]
Match NameE-valueIdentityDescription
AT4G25630.11.8e-12583.66fibrillarin 2 [more]
AT5G52470.11.7e-12383.17fibrillarin 1 [more]
AT5G52490.15.2e-9366.55Fibrillarin family protein [more]
AT5G52470.25.7e-8482.88fibrillarin 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000692FibrillarinPRINTSPR00052FIBRILLARINcoord: 109..128
score: 75.71
coord: 161..178
score: 72.22
coord: 268..289
score: 61.36
coord: 81..100
score: 64.29
coord: 216..245
score: 67.62
coord: 186..215
score: 69.29
coord: 136..157
score: 77.92
IPR000692FibrillarinSMARTSM01206Fibrillarin_2coord: 63..291
e-value: 1.2E-172
score: 589.5
IPR000692FibrillarinPIRSFPIRSF006540Nop17pcoord: 13..293
e-value: 1.1E-120
score: 399.5
IPR000692FibrillarinPFAMPF01269Fibrillarincoord: 63..290
e-value: 2.2E-109
score: 363.9
IPR000692FibrillarinHAMAPMF_00351RNA_methyltransf_FlpAcoord: 77..290
score: 36.871891
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 58..113
e-value: 1.5E-29
score: 103.6
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 114..291
e-value: 2.2E-88
score: 296.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..60
NoneNo IPR availablePANTHERPTHR10335:SF22FIBRILLARIN, S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-RELATEDcoord: 1..298
NoneNo IPR availablePANTHERPTHR10335RRNA 2-O-METHYLTRANSFERASE FIBRILLARINcoord: 1..298
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 138..240
e-value: 1.04207E-4
score: 38.9503
IPR020813Fibrillarin, conserved sitePROSITEPS00566FIBRILLARINcoord: 161..175
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 61..289

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC10g1161MC10g1161gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC10g1161.1-cdsMC10g1161.1-cds-MC10:14666183..14666485CDS
MC10g1161.1-cdsMC10g1161.1-cds-MC10:14666760..14666855CDS
MC10g1161.1-cdsMC10g1161.1-cds-MC10:14666959..14667036CDS
MC10g1161.1-cdsMC10g1161.1-cds-MC10:14667118..14667294CDS
MC10g1161.1-cdsMC10g1161.1-cds-MC10:14669049..14669117CDS
MC10g1161.1-cdsMC10g1161.1-cds-MC10:14669203..14669379CDS


The following utr3 feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC10g1161.1-utr3MC10g1161.1-utr3-MC10:14669380..14669648utr3


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC10g1161.1MC10g1161.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006364 rRNA processing
biological_process GO:0000494 box C/D RNA 3'-end processing
biological_process GO:0031122 cytoplasmic microtubule organization
biological_process GO:1990258 histone glutamine methylation
biological_process GO:0051321 meiotic cell cycle
biological_process GO:0007020 microtubule nucleation
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0031167 rRNA methylation
biological_process GO:0051225 spindle assembly
cellular_component GO:0005874 microtubule
cellular_component GO:0000922 spindle pole
cellular_component GO:0032040 small-subunit processome
cellular_component GO:0005730 nucleolus
cellular_component GO:0031428 box C/D RNP complex
cellular_component GO:0000930 gamma-tubulin complex
cellular_component GO:0000923 equatorial microtubule organizing center
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0043015 gamma-tubulin binding
molecular_function GO:1990259 histone-glutamine methyltransferase activity
molecular_function GO:0051011 microtubule minus-end binding
molecular_function GO:0008649 rRNA methyltransferase activity