MC10g0013.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC10g0013.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionCalcium-binding endonuclease/exonuclease/phosphatase family
LocationMC10: 108807 .. 112983 (+)
Sequence length2220
RNA-Seq ExpressionMC10g0013.1
SyntenyMC10g0013.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCCCACCCGACCAGGACCAAGAGGAGACCAAGAGAAGAGGTCCTTTTTCTCTCAAATATCACTTCTAAGTTTCAGTTTTCTTTTTAATTCTTTTATATATATCAATTATAAATAGGCATAGCCTAAGCTTGGGCCTGGGCGTTCCGCGAGAGATTGAGATAAGATTAAACGCGGGTGCTGGTGCTGGTGCTGGTGCTGCTGCTCCTGCTCCCCTCTCCTCTCCTCTCAGGCTCAGGCTCACCGCTTCCCCCTTCCTCTTCCGCTTCTGCCGGTTTTTTTACAGTTACTTTCTTCGCTGTCCAAATTTAATTATTTTGCCTAACCCTAACCCCCTCGCCTTTCCCTTCCCAATTCCAATTCACTTTTCATCTCTCTCTTTCTCTTTCTGGGGAAAGAAAAGTCTTTTCTCCTTCGTTGTGGTGAAAGAAAAGTCTCCTCTCTTTCATATCACGAATCGCGATCACCCCACTTGTCGTTTACGCCCATTATTTGATTCCAAGCAGGAAACCATTTTTTTAATATTTCTTCTACAGCATTTGCATTTCACGCTCCCGCTGGTTTTGTTATATAAGTAGATCGTACACTGGAGGGCCAGGAATCAAGAATGAGGAAGGAGAGAAGAAGAGATAAGAAGATGAAAGGAAGGATTTCAATGATTGGGAGCTACGCCATTGCTTCCTCCATTAAAGACCAGCATCATCAACGCCCTTGCATCACCTGCACTACCTTTAATATACTCGCTCCCATCTACAAGCGTCTCAACCAACAGGTTCCCCTCCATCCATCTTTCCCTTCAATTTTTATCTTTCTCCATTCCGATTCCCATTCATTTTCCATGAAAAAAACAGGATCCCAGTTGCCGTGAGAGCGATTACAGGACATACTGGCTAGCCAGAAATCAGAGGATACTCGATTGGCTCTTGTATGAGAAATCTTCCATTATCTGCCTTCAGGTTTCCATTTCTTTTCTAAGTCCATCTTTGTCTAATTCTTTCCGGGAAAACAAGGTCTCAAAACAATCGTTGATGTGTTTGTGTGTCCTTTTCTTTTCTTCTGTGGCCGTGAGCAGGAATTCTGGGTTGGAAACGAAGAACTTGTAAATATATACGAAAACCGACTAGCCAATGCCGGTTACCTCAGTTTCAAGCTTGCTCGGACCAATAACCGTGGTGATGGTACGTTTTTCTTTACCAATTCATTCCACCCCCTTTCCCTTCTTTCTTATACCAAGGATTGTTCGAGTGTACCAATCCAAATTCATTGTCCTTCAGAGTTCCTCGTCTTGGGTTTTCTCTTTTACTTCATGAATATTTGAGTCCACATTTAACACCAGGAATTGATGATTATGATGATTTGTGTATTGGTATTATGTTTATTAGCCGTGAAACCATTTTAAAGTGAAGTTGTTTGGATGTGAAACTACAGGTTTGTTGACTGCGGTGAATAAGGATTATTTTAAAGTGGTAAACTACCGAGAGCTGCTATTTAACGATTTTGGAGATCGCGTTGCTCAGCTATTGCATGTTGAATTAGCAGTTCCATTCTCACAGTGCCGGAACAATGATATCCGTCAGGAAATTCTCATTGTCAACACTCACTTGTTATTTCCTCACGATTCTAGTCTCTGTCTTGTCCGGTTGAATCAAGTAAGTTTGACTCACTGTCCTTGGATTCATGCAACTTCTTGCACTTTCCATTTCCAATTTTAATGATCTTAACCTTATTCTTTTCTCCAGGTATACAAAATCCTACAATATGTTGAATCTTATCAGAAAGAAAACAAACTCAATCCCATGCCCATCATACTTTGCGGGTAAACCTCATTCCCTGATCTTCATTCTTGTTCTTGTTTGCCCATCATCTTCTTCTTTTGTGGTAATTTCGAGATGGTTGTTTTGTTCTGTTTAGTGACTGGAACGGAAGCAAACGTGGGCATGTGTACAAGTTTCTAAGGTCGCAGGGGTTTGTATCCTCCTATGACACAGCTCATCAGTACACTGATGCAGATGCACGCAAGGTTAGCATCTTCAATTTTATTCCCTCATCCTCATCTAAAACTTAAAATTAATAGTCAACGTAGACTATTTTTCTAATCTTGGTTGTGGTTGTGGCTTTCATGTATGTAAACCAGTGGGTGAGTCACCGGAATCATCGGGGAAACATATGTGGGGTTGACTTCATTTGGCTTTTAAATCCTAATGGATACAGAAAATTGCTAAAAACTAGCTGGAGTGAAGCTGTTTTTGGCATGTTCAAGGTAACCACTTCTCCATTTCTGCCTGTTCTCCTCCCACACTAATTGCTGTGTCTTTCAAGTTTCAATTTATGAGCATACCTACGACTTATTTGTCTCGTACTATCATAATCATTTCCTTGTTTAAATTTTCAATTAGGATATATTATCTCTAGTACCTTGAGCATCAACCACTTGACTGCAGTTCTTCACTTTCCTTCATTATATTGCATGACGGACTTATGTTATCTGCTGGATTTGAAAACAGAGGGGACGTATGTGAATAATCAACTATTTAACAGCACTCTTCAGTTTCACTCGTCCAAAATTTATCACAAACACACTATTGACTATTGAGCCATGATATGAATTATTTAATTCTGCAATGCTATTTAATTGTAGTCCCTTGCTCTGCGGTGGCTACTTGAATTGTTTTATATCTGCATTTCTAATACAATCTTACCATTTTCAGTACCTACTTCGAAGAGCTTCACTTACAGAAGATGATGCATTTGCCTTTCTGCAGGCGGACAATGATGGTGACTATATAACCTACTCAGGTTTCTGTGAGGCACTTCGACAGGTATATAAGGCTTGGCTTGCTGATTTTGGGTTTGAAAATGTGGAACCTGTGTTGAATGTGATTGGGAGAAAGATCCTTGAATCGAAAAACTTATTTATGAATGCTTAATCATTTTGTTCAGCTCAATTTAACTGGGCATCCCCATGGGCTTAGCGTTGGAGAGATAGAGGACTTGTGGGTACAAGCAGATATTGATGGAAACGGCATTCTCGATTATAATGAATTTCAGGTAAATTTGTTGTCCTTTTGACACTGACATAAGGTAAAGTAGATAAAAAAAGTTGAAGAGCAATTGAGATTGATAGTAATGGGGTGGGTGTTTCGTGGTTTCTGACAGCAGAGAATTTGGAATTCTACGGGGAACGAGGAGAGGGATGGAAGAAGCAACGAGGTGGGGAGTGGTGGGGAGTCAAAGGAAAATCAGGAGCAAACGATTGGTTTCAGTGTTGAAAATGCGGTGCTGTTTCCTGCAGAAGTGGAGAAAGGAAGGTGGCCCGAGGACTACTCGCTTTCAGATCATGCACGACTCACTGTGGTGTTCGCACCCATAAGAATGCCCTGTTCACAGTTGATATCCTGACGCGGGCCTCGCCATTTTTCCTTTGCCACCCTCAACAGCTTTGCGTTGTTGAATCTCGCACCAGCACCAGCAGATGAAAACTTGGCGTCACCATTTTTGGGGATGGGGAGCATCCGCATCCACATCTACACACTACACTACAGTTGAATAAGTAAGAAAAGGATAGGAAAGAGCTGATTAAGTGTACAGACAGTTACAGTTACAGTTACAGTTACAGTTGCAGTTATATGGGTTTTAGAAATCGTGGCTTTTGTAATTTTAGTGCCGTATACATATAGGAGGCAGGAGCAACATTTGTACAGTAAATGAATTCATGGAAGTCCCGGATGGAGGAGCAGAGCATAGCATACCAGTACCATTACCATTATATGGTTATTACTGTAGAAATATATGCAAATGCAAATGGATGCTTAAAGGTGATGTTTTTAAAGAGACAAACAGTTGGCATTCCAATCCCTTCCTTAATGAACAAGGAAATCAAATATCGTTTCCCCAGTCCCAGTACCAGTCCCAGATTAAGCATAAAGGTGCTCCGCCAGCACCGCTCTGTCTTTTACATTCAAATACATTTGATTTTCCTTGGGAACCAAAAGTCGCTTCTTTGTCTGATTCTCTTCTGGGTAAATCGTAATTCATGGCACTGTCTTATTATGACAATTCTACCACAAGCCACTCCATTTGCTCACTCCCAACACCCAATTCCTTTTTCTTTTTATAAAATACCATAATAGAAGTACTGATTTTTGTATAAGCGAAAAATCAACGCTCAAAATGCA

mRNA sequence

ATGAATCCCACCCGACCAGGACCAAGAGGAGACCAAGAGAAGAGATCGTACACTGGAGGGCCAGGAATCAAGAATGAGGAAGGAGAGAAGAAGAGATAAGAAGATGAAAGGAAGGATTTCAATGATTGGGAGCTACGCCATTGCTTCCTCCATTAAAGACCAGCATCATCAACGCCCTTGCATCACCTGCACTACCTTTAATATACTCGCTCCCATCTACAAGCGTCTCAACCAACAGGATCCCAGTTGCCGTGAGAGCGATTACAGGACATACTGGCTAGCCAGAAATCAGAGGATACTCGATTGGCTCTTGTATGAGAAATCTTCCATTATCTGCCTTCAGGAATTCTGGGTTGGAAACGAAGAACTTGTAAATATATACGAAAACCGACTAGCCAATGCCGGTTACCTCAGTTTCAAGCTTGCTCGGACCAATAACCGTGGTGATGGTTTGTTGACTGCGGTGAATAAGGATTATTTTAAAGTGGTAAACTACCGAGAGCTGCTATTTAACGATTTTGGAGATCGCGTTGCTCAGCTATTGCATGTTGAATTAGCAGTTCCATTCTCACAGTGCCGGAACAATGATATCCGTCAGGAAATTCTCATTGTCAACACTCACTTGTTATTTCCTCACGATTCTAGTCTCTGTCTTGTCCGGTTGAATCAAGTATACAAAATCCTACAATATGTTGAATCTTATCAGAAAGAAAACAAACTCAATCCCATGCCCATCATACTTTGCGGTGACTGGAACGGAAGCAAACGTGGGCATGTGTACAAGTTTCTAAGGTCGCAGGGGTTTGTATCCTCCTATGACACAGCTCATCAGTACACTGATGCAGATGCACGCAAGTGGGTGAGTCACCGGAATCATCGGGGAAACATATGTGGGGTTGACTTCATTTGGCTTTTAAATCCTAATGGATACAGAAAATTGCTAAAAACTAGCTGGAGTGAAGCTGTTTTTGGCATGTTCAAGTACCTACTTCGAAGAGCTTCACTTACAGAAGATGATGCATTTGCCTTTCTGCAGGCGGACAATGATGGTGACTATATAACCTACTCAGGTTTCTGTGAGGCACTTCGACAGCTCAATTTAACTGGGCATCCCCATGGGCTTAGCGTTGGAGAGATAGAGGACTTGTGGGTACAAGCAGATATTGATGGAAACGGCATTCTCGATTATAATGAATTTCAGCAGAGAATTTGGAATTCTACGGGGAACGAGGAGAGGGATGGAAGAAGCAACGAGGTGGGGAGTGGTGGGGAGTCAAAGGAAAATCAGGAGCAAACGATTGGTTTCAGTGTTGAAAATGCGGTGCTGTTTCCTGCAGAAGTGGAGAAAGGAAGGTGGCCCGAGGACTACTCGCTTTCAGATCATGCACGACTCACTGTGGTGTTCGCACCCATAAGAATGCCCTGTTCACAGTTGATATCCTGACGCGGGCCTCGCCATTTTTCCTTTGCCACCCTCAACAGCTTTGCGTTGTTGAATCTCGCACCAGCACCAGCAGATGAAAACTTGGCGTCACCATTTTTGGGGATGGGGAGCATCCGCATCCACATCTACACACTACACTACAGTTGAATAAGTAAGAAAAGGATAGGAAAGAGCTGATTAAGTGTACAGACAGTTACAGTTACAGTTACAGTTACAGTTGCAGTTATATGGGTTTTAGAAATCGTGGCTTTTGTAATTTTAGTGCCGTATACATATAGGAGGCAGGAGCAACATTTGTACAGTAAATGAATTCATGGAAGTCCCGGATGGAGGAGCAGAGCATAGCATACCAGTACCATTACCATTATATGGTTATTACTGTAGAAATATATGCAAATGCAAATGGATGCTTAAAGGTGATGTTTTTAAAGAGACAAACAGTTGGCATTCCAATCCCTTCCTTAATGAACAAGGAAATCAAATATCGTTTCCCCAGTCCCAGTACCAGTCCCAGATTAAGCATAAAGGTGCTCCGCCAGCACCGCTCTGTCTTTTACATTCAAATACATTTGATTTTCCTTGGGAACCAAAAGTCGCTTCTTTGTCTGATTCTCTTCTGGGTAAATCGTAATTCATGGCACTGTCTTATTATGACAATTCTACCACAAGCCACTCCATTTGCTCACTCCCAACACCCAATTCCTTTTTCTTTTTATAAAATACCATAATAGAAGTACTGATTTTTGTATAAGCGAAAAATCAACGCTCAAAATGCA

Coding sequence (CDS)

ATGAGGAAGGAGAGAAGAAGAGATAAGAAGATGAAAGGAAGGATTTCAATGATTGGGAGCTACGCCATTGCTTCCTCCATTAAAGACCAGCATCATCAACGCCCTTGCATCACCTGCACTACCTTTAATATACTCGCTCCCATCTACAAGCGTCTCAACCAACAGGATCCCAGTTGCCGTGAGAGCGATTACAGGACATACTGGCTAGCCAGAAATCAGAGGATACTCGATTGGCTCTTGTATGAGAAATCTTCCATTATCTGCCTTCAGGAATTCTGGGTTGGAAACGAAGAACTTGTAAATATATACGAAAACCGACTAGCCAATGCCGGTTACCTCAGTTTCAAGCTTGCTCGGACCAATAACCGTGGTGATGGTTTGTTGACTGCGGTGAATAAGGATTATTTTAAAGTGGTAAACTACCGAGAGCTGCTATTTAACGATTTTGGAGATCGCGTTGCTCAGCTATTGCATGTTGAATTAGCAGTTCCATTCTCACAGTGCCGGAACAATGATATCCGTCAGGAAATTCTCATTGTCAACACTCACTTGTTATTTCCTCACGATTCTAGTCTCTGTCTTGTCCGGTTGAATCAAGTATACAAAATCCTACAATATGTTGAATCTTATCAGAAAGAAAACAAACTCAATCCCATGCCCATCATACTTTGCGGTGACTGGAACGGAAGCAAACGTGGGCATGTGTACAAGTTTCTAAGGTCGCAGGGGTTTGTATCCTCCTATGACACAGCTCATCAGTACACTGATGCAGATGCACGCAAGTGGGTGAGTCACCGGAATCATCGGGGAAACATATGTGGGGTTGACTTCATTTGGCTTTTAAATCCTAATGGATACAGAAAATTGCTAAAAACTAGCTGGAGTGAAGCTGTTTTTGGCATGTTCAAGTACCTACTTCGAAGAGCTTCACTTACAGAAGATGATGCATTTGCCTTTCTGCAGGCGGACAATGATGGTGACTATATAACCTACTCAGGTTTCTGTGAGGCACTTCGACAGCTCAATTTAACTGGGCATCCCCATGGGCTTAGCGTTGGAGAGATAGAGGACTTGTGGGTACAAGCAGATATTGATGGAAACGGCATTCTCGATTATAATGAATTTCAGCAGAGAATTTGGAATTCTACGGGGAACGAGGAGAGGGATGGAAGAAGCAACGAGGTGGGGAGTGGTGGGGAGTCAAAGGAAAATCAGGAGCAAACGATTGGTTTCAGTGTTGAAAATGCGGTGCTGTTTCCTGCAGAAGTGGAGAAAGGAAGGTGGCCCGAGGACTACTCGCTTTCAGATCATGCACGACTCACTGTGGTGTTCGCACCCATAAGAATGCCCTGTTCACAGTTGATATCCTGA

Protein sequence

MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Homology
BLAST of MC10g0013.1 vs. ExPASy Swiss-Prot
Match: O81916 (Uncharacterized calcium-binding protein At1g02270 OS=Arabidopsis thaliana OX=3702 GN=At1g02270 PE=2 SV=2)

HSP 1 Score: 596.7 bits (1537), Expect = 2.3e-169
Identity = 278/416 (66.83%), Postives = 343/416 (82.45%), Query Frame = 0

Query: 37  ITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGN 96
           I+CTTFNILAPIYKR++Q++ S RESD+RT WLARNQRILD LL+++SS+ICLQE WVGN
Sbjct: 71  ISCTTFNILAPIYKRVDQKNHSTRESDFRTLWLARNQRILDLLLHQRSSVICLQEVWVGN 130

Query: 97  EELVNIYENRLANAGYLSFKLARTNNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQL 156
           EELVN+Y ++L+++GY  ++LARTN+RGDGLLTA++KD+FKVVNYRELLFNDFGDRVAQL
Sbjct: 131 EELVNMYHHQLSSSGYTIYQLARTNSRGDGLLTAIHKDHFKVVNYRELLFNDFGDRVAQL 190

Query: 157 LHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKL 216
           LHV+  +PF      D++QE++IVNTHLLFPHDSSL +VRL+QVYKIL+Y+E+YQKENKL
Sbjct: 191 LHVKTVIPFPLNGKQDVQQEVIIVNTHLLFPHDSSLSIVRLHQVYKILEYLEAYQKENKL 250

Query: 217 NPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVD 276
           N MPIILCGDWNGSKRGHVYKFLRSQGF+SSYD AHQYTD+DA +WVSHRNHRGNICGVD
Sbjct: 251 NHMPIILCGDWNGSKRGHVYKFLRSQGFISSYDDAHQYTDSDAHRWVSHRNHRGNICGVD 310

Query: 277 FIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCE 336
           FIWL NP+  RK L+TSW EAVF + KY L +AS+ EDDAF FL A N  D +TYS FC 
Sbjct: 311 FIWLCNPSDSRKPLRTSWVEAVFSIIKYQLHKASIAEDDAFTFLGAKNHSDSLTYSDFCL 370

Query: 337 ALRQLNLTGHPHGLSVGEIEDLWVQADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVG 396
           AL+++NLTG PHGLS  E ++LWV+AD+DGNG+ DY E  ++IWN T    + G   E  
Sbjct: 371 ALQKVNLTGIPHGLSFEETKELWVRADLDGNGVFDYEEL-KKIWNMT-MVNQPGNCKESV 430

Query: 397 SGGESKENQEQTIGFSVENAVLFPAEVEKGRWPEDYSLSDHARLTVVFAPIRMPCS 453
              + +E +++ IG  V  A+LFP E EKG WPE+Y++SDHA LTV F+P++M CS
Sbjct: 431 MESKKEEGEDEAIGLKVNKAILFPQEAEKGLWPENYNISDHACLTVQFSPVKMLCS 484

BLAST of MC10g0013.1 vs. ExPASy Swiss-Prot
Match: Q9LS39 (Carbon catabolite repressor protein 4 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CCR4-3 PE=2 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 6.9e-09
Identity = 51/177 (28.81%), Postives = 86/177 (48.59%), Query Frame = 0

Query: 68  WLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGL 127
           W  R + I + L+     II +QE     ++  +++ + +  AGY      RT +  DG 
Sbjct: 139 WGYRKRLICEELIRLNPDIISMQEV----DKYFDLF-SMMEKAGYAGSYKRRTGDNVDGC 198

Query: 128 LTAVNKDYFKVVNYRELLFNDFG--DRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLL 187
                 D F V+    + F+ FG  D VAQL  +EL       R ++  ++IL+ N H+L
Sbjct: 199 AMFWKADRFGVLERENIEFSQFGMRDNVAQLAVLEL-------RKSNKSRKILLGNIHVL 258

Query: 188 F-PHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRS 242
           + P+      V+L QV  +    +++    K   +PI+LCGD+N + +  +Y FL S
Sbjct: 259 YNPNQGD---VKLGQVRSLCS--KAHLLSKKWGDIPIVLCGDFNSTPKSPLYNFLAS 298

BLAST of MC10g0013.1 vs. NCBI nr
Match: XP_022141208.1 (uncharacterized calcium-binding protein At1g02270 isoform X1 [Momordica charantia])

HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456

BLAST of MC10g0013.1 vs. NCBI nr
Match: XP_022141209.1 (uncharacterized calcium-binding protein At1g02270 isoform X2 [Momordica charantia])

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 455/456 (99.78%), Postives = 455/456 (99.78%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QADIDGNGILDYNEFQ RIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADIDGNGILDYNEFQ-RIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 455

BLAST of MC10g0013.1 vs. NCBI nr
Match: XP_022141210.1 (uncharacterized calcium-binding protein At1g02270 isoform X3 [Momordica charantia])

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 438/456 (96.05%), Postives = 438/456 (96.05%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFK                  ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV
Sbjct: 301 MFK------------------ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 438

BLAST of MC10g0013.1 vs. NCBI nr
Match: XP_022923111.1 (uncharacterized calcium-binding protein At1g02270 isoform X1 [Cucurbita moschata])

HSP 1 Score: 891 bits (2303), Expect = 0.0
Identity = 435/456 (95.39%), Postives = 443/456 (97.15%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRL NAGY+SFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLRNAGYISFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QAD DGNGILD+NEFQQRIWNSTG EERD + NEV    ESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADSDGNGILDHNEFQQRIWNSTGTEERDEKGNEV----ESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MC10g0013.1 vs. NCBI nr
Match: XP_022984360.1 (uncharacterized calcium-binding protein At1g02270 isoform X1 [Cucurbita maxima] >KAG7014667.1 putative calcium-binding protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 435/456 (95.39%), Postives = 444/456 (97.37%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRL+QQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLSQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRL NAGY+SFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLRNAGYISFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QAD DGNGILD+NEFQQRIWNSTG EERD +SNEV    ESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADSDGNGILDHNEFQQRIWNSTGTEERDEKSNEV----ESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MC10g0013.1 vs. ExPASy TrEMBL
Match: A0A6J1CJT7 (uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011664 PE=4 SV=1)

HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456

BLAST of MC10g0013.1 vs. ExPASy TrEMBL
Match: A0A6J1CHY0 (uncharacterized calcium-binding protein At1g02270 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011664 PE=4 SV=1)

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 455/456 (99.78%), Postives = 455/456 (99.78%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QADIDGNGILDYNEFQ RIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADIDGNGILDYNEFQ-RIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 455

BLAST of MC10g0013.1 vs. ExPASy TrEMBL
Match: A0A6J1CHC3 (uncharacterized calcium-binding protein At1g02270 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011664 PE=4 SV=1)

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 438/456 (96.05%), Postives = 438/456 (96.05%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFK                  ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV
Sbjct: 301 MFK------------------ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 438

BLAST of MC10g0013.1 vs. ExPASy TrEMBL
Match: A0A6J1E5X5 (uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430875 PE=4 SV=1)

HSP 1 Score: 891 bits (2303), Expect = 0.0
Identity = 435/456 (95.39%), Postives = 443/456 (97.15%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRLNQQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLNQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRL NAGY+SFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLRNAGYISFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QAD DGNGILD+NEFQQRIWNSTG EERD + NEV    ESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADSDGNGILDHNEFQQRIWNSTGTEERDEKGNEV----ESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MC10g0013.1 vs. ExPASy TrEMBL
Match: A0A6J1J8E9 (uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482685 PE=4 SV=1)

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 435/456 (95.39%), Postives = 444/456 (97.37%), Query Frame = 0

Query: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCR 60
           MRKERRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRL+QQDPSCR
Sbjct: 1   MRKERRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLSQQDPSCR 60

Query: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLART 120
           ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRL NAGY+SFKLART
Sbjct: 61  ESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLRNAGYISFKLART 120

Query: 121 NNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180
           NNRGDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIV
Sbjct: 121 NNRGDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIV 180

Query: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240
           NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR
Sbjct: 181 NTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLR 240

Query: 241 SQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300
           SQGFVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG
Sbjct: 241 SQGFVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFG 300

Query: 301 MFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWV 360
           MFKYLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWV
Sbjct: 301 MFKYLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWV 360

Query: 361 QADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFP 420
           QAD DGNGILD+NEFQQRIWNSTG EERD +SNEV    ESKENQEQTIGFSVENAVLFP
Sbjct: 361 QADSDGNGILDHNEFQQRIWNSTGTEERDEKSNEV----ESKENQEQTIGFSVENAVLFP 420

Query: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456
           AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 421 AEVEKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MC10g0013.1 vs. TAIR 10
Match: AT5G54130.2 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 742.7 bits (1916), Expect = 1.8e-214
Identity = 356/446 (79.82%), Postives = 394/446 (88.34%), Query Frame = 0

Query: 11  MKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLA 70
           MKGRIS IGSYAI+SSI+DQ HQ+PCI+CTTFNILAPIYKRL+ +D S RESD R YWL 
Sbjct: 1   MKGRISKIGSYAISSSIRDQ-HQQPCISCTTFNILAPIYKRLSHKDQSLRESDNRAYWLG 60

Query: 71  RNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGLLTA 130
           RN RI+DWLLYE+SSIICLQEFWVGNEELVN+YE RL +AGYLS+KL RTNNRGDGLLTA
Sbjct: 61  RNHRIIDWLLYERSSIICLQEFWVGNEELVNLYEKRLGDAGYLSYKLGRTNNRGDGLLTA 120

Query: 131 VNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDS 190
           V+KDYF+VVN R+LLFND GDRVAQLLHVEL  P+SQ    D  QE+LIVNTHLLFPHDS
Sbjct: 121 VHKDYFRVVNSRDLLFNDCGDRVAQLLHVELVPPYSQ---YDAHQEVLIVNTHLLFPHDS 180

Query: 191 SLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT 250
           +L +VRL QVYKILQYVESYQKE  L+PMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT
Sbjct: 181 TLSIVRLQQVYKILQYVESYQKEVNLSPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT 240

Query: 251 AHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRAS 310
           AH+YTD+DA KWVSHRNHRGNIC VDFIWLLNPN YRKLLKTSWSEAVFGMF+YLLRRAS
Sbjct: 241 AHRYTDSDAHKWVSHRNHRGNICAVDFIWLLNPNRYRKLLKTSWSEAVFGMFRYLLRRAS 300

Query: 311 LTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQADIDGNGIL 370
           LT +DAFAFL+ DNDGD+IT+ GFCE LRQLNLTGH +GL+  EI+DLW QADIDGNG+L
Sbjct: 301 LTAEDAFAFLKTDNDGDHITFMGFCETLRQLNLTGHCNGLTTKEIKDLWTQADIDGNGLL 360

Query: 371 DYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEVEKGRWPE 430
           DY EFQQRIWN T +E+RD         GE+K NQEQT+GFSV+NAVLFP EVEKG WPE
Sbjct: 361 DYKEFQQRIWNQTWSEQRDAED------GEAKGNQEQTVGFSVKNAVLFPPEVEKGMWPE 420

Query: 431 DYSLSDHARLTVVFAPIRMPCSQLIS 457
           +YSLSDHARLTVVF+PIRMPCSQL+S
Sbjct: 421 NYSLSDHARLTVVFSPIRMPCSQLVS 436

BLAST of MC10g0013.1 vs. TAIR 10
Match: AT1G02270.1 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 596.7 bits (1537), Expect = 1.6e-170
Identity = 278/416 (66.83%), Postives = 343/416 (82.45%), Query Frame = 0

Query: 37  ITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLARNQRILDWLLYEKSSIICLQEFWVGN 96
           I+CTTFNILAPIYKR++Q++ S RESD+RT WLARNQRILD LL+++SS+ICLQE WVGN
Sbjct: 71  ISCTTFNILAPIYKRVDQKNHSTRESDFRTLWLARNQRILDLLLHQRSSVICLQEVWVGN 130

Query: 97  EELVNIYENRLANAGYLSFKLARTNNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQL 156
           EELVN+Y ++L+++GY  ++LARTN+RGDGLLTA++KD+FKVVNYRELLFNDFGDRVAQL
Sbjct: 131 EELVNMYHHQLSSSGYTIYQLARTNSRGDGLLTAIHKDHFKVVNYRELLFNDFGDRVAQL 190

Query: 157 LHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENKL 216
           LHV+  +PF      D++QE++IVNTHLLFPHDSSL +VRL+QVYKIL+Y+E+YQKENKL
Sbjct: 191 LHVKTVIPFPLNGKQDVQQEVIIVNTHLLFPHDSSLSIVRLHQVYKILEYLEAYQKENKL 250

Query: 217 NPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGVD 276
           N MPIILCGDWNGSKRGHVYKFLRSQGF+SSYD AHQYTD+DA +WVSHRNHRGNICGVD
Sbjct: 251 NHMPIILCGDWNGSKRGHVYKFLRSQGFISSYDDAHQYTDSDAHRWVSHRNHRGNICGVD 310

Query: 277 FIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRASLTEDDAFAFLQADNDGDYITYSGFCE 336
           FIWL NP+  RK L+TSW EAVF + KY L +AS+ EDDAF FL A N  D +TYS FC 
Sbjct: 311 FIWLCNPSDSRKPLRTSWVEAVFSIIKYQLHKASIAEDDAFTFLGAKNHSDSLTYSDFCL 370

Query: 337 ALRQLNLTGHPHGLSVGEIEDLWVQADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEVG 396
           AL+++NLTG PHGLS  E ++LWV+AD+DGNG+ DY E  ++IWN T    + G   E  
Sbjct: 371 ALQKVNLTGIPHGLSFEETKELWVRADLDGNGVFDYEEL-KKIWNMT-MVNQPGNCKESV 430

Query: 397 SGGESKENQEQTIGFSVENAVLFPAEVEKGRWPEDYSLSDHARLTVVFAPIRMPCS 453
              + +E +++ IG  V  A+LFP E EKG WPE+Y++SDHA LTV F+P++M CS
Sbjct: 431 MESKKEEGEDEAIGLKVNKAILFPQEAEKGLWPENYNISDHACLTVQFSPVKMLCS 484

BLAST of MC10g0013.1 vs. TAIR 10
Match: AT5G54130.4 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 570.5 bits (1469), Expect = 1.2e-162
Identity = 271/333 (81.38%), Postives = 299/333 (89.79%), Query Frame = 0

Query: 11  MKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLA 70
           MKGRIS IGSYAI+SSI+DQ HQ+PCI+CTTFNILAPIYKRL+ +D S RESD R YWL 
Sbjct: 1   MKGRISKIGSYAISSSIRDQ-HQQPCISCTTFNILAPIYKRLSHKDQSLRESDNRAYWLG 60

Query: 71  RNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGLLTA 130
           RN RI+DWLLYE+SSIICLQEFWVGNEELVN+YE RL +AGYLS+KL RTNNRGDGLLTA
Sbjct: 61  RNHRIIDWLLYERSSIICLQEFWVGNEELVNLYEKRLGDAGYLSYKLGRTNNRGDGLLTA 120

Query: 131 VNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDS 190
           V+KDYF+VVN R+LLFND GDRVAQLLHVEL  P+SQ    D  QE+LIVNTHLLFPHDS
Sbjct: 121 VHKDYFRVVNSRDLLFNDCGDRVAQLLHVELVPPYSQ---YDAHQEVLIVNTHLLFPHDS 180

Query: 191 SLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT 250
           +L +VRL QVYKILQYVESYQKE  L+PMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT
Sbjct: 181 TLSIVRLQQVYKILQYVESYQKEVNLSPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT 240

Query: 251 AHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRAS 310
           AH+YTD+DA KWVSHRNHRGNIC VDFIWLLNPN YRKLLKTSWSEAVFGMF+YLLRRAS
Sbjct: 241 AHRYTDSDAHKWVSHRNHRGNICAVDFIWLLNPNRYRKLLKTSWSEAVFGMFRYLLRRAS 300

Query: 311 LTEDDAFAFLQADNDGDYITYSGFCEALRQLNL 344
           LT +DAFAFL+ DNDGD+IT+ GFCE LRQ+ +
Sbjct: 301 LTAEDAFAFLKTDNDGDHITFMGFCETLRQVTV 329

BLAST of MC10g0013.1 vs. TAIR 10
Match: AT5G54130.3 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 560.8 bits (1444), Expect = 9.8e-160
Identity = 269/333 (80.78%), Postives = 297/333 (89.19%), Query Frame = 0

Query: 11  MKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLA 70
           MKGRIS IGSYAI+SSI+DQ HQ+PCI+CTTFNILAPIYKRL+ +D S RESD R YWL 
Sbjct: 1   MKGRISKIGSYAISSSIRDQ-HQQPCISCTTFNILAPIYKRLSHKDQSLRESDNRAYWLG 60

Query: 71  RNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGLLTA 130
           RN RI+DWLLYE+SSIICLQEFWVGNEELVN+YE RL +AGYLS+KL RTNNR  GLLTA
Sbjct: 61  RNHRIIDWLLYERSSIICLQEFWVGNEELVNLYEKRLGDAGYLSYKLGRTNNR--GLLTA 120

Query: 131 VNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDS 190
           V+KDYF+VVN R+LLFND GDRVAQLLHVEL  P+SQ    D  QE+LIVNTHLLFPHDS
Sbjct: 121 VHKDYFRVVNSRDLLFNDCGDRVAQLLHVELVPPYSQ---YDAHQEVLIVNTHLLFPHDS 180

Query: 191 SLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT 250
           +L +VRL QVYKILQYVESYQKE  L+PMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT
Sbjct: 181 TLSIVRLQQVYKILQYVESYQKEVNLSPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDT 240

Query: 251 AHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRAS 310
           AH+YTD+DA KWVSHRNHRGNIC VDFIWLLNPN YRKLLKTSWSEAVFGMF+YLLRRAS
Sbjct: 241 AHRYTDSDAHKWVSHRNHRGNICAVDFIWLLNPNRYRKLLKTSWSEAVFGMFRYLLRRAS 300

Query: 311 LTEDDAFAFLQADNDGDYITYSGFCEALRQLNL 344
           LT +DAFAFL+ DNDGD+IT+ GFCE LRQ+ +
Sbjct: 301 LTAEDAFAFLKTDNDGDHITFMGFCETLRQVTV 327

BLAST of MC10g0013.1 vs. TAIR 10
Match: AT3G18500.2 (DNAse I-like superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 4.9e-10
Identity = 51/177 (28.81%), Postives = 86/177 (48.59%), Query Frame = 0

Query: 68  WLARNQRILDWLLYEKSSIICLQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGL 127
           W  R + I + L+     II +QE     ++  +++ + +  AGY      RT +  DG 
Sbjct: 139 WGYRKRLICEELIRLNPDIISMQEV----DKYFDLF-SMMEKAGYAGSYKRRTGDNVDGC 198

Query: 128 LTAVNKDYFKVVNYRELLFNDFG--DRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLL 187
                 D F V+    + F+ FG  D VAQL  +EL       R ++  ++IL+ N H+L
Sbjct: 199 AMFWKADRFGVLERENIEFSQFGMRDNVAQLAVLEL-------RKSNKSRKILLGNIHVL 258

Query: 188 F-PHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRS 242
           + P+      V+L QV  +    +++    K   +PI+LCGD+N + +  +Y FL S
Sbjct: 259 YNPNQGD---VKLGQVRSLCS--KAHLLSKKWGDIPIVLCGDFNSTPKSPLYNFLAS 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O819162.3e-16966.83Uncharacterized calcium-binding protein At1g02270 OS=Arabidopsis thaliana OX=370... [more]
Q9LS396.9e-0928.81Carbon catabolite repressor protein 4 homolog 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_022141208.10.0100.00uncharacterized calcium-binding protein At1g02270 isoform X1 [Momordica charanti... [more]
XP_022141209.10.099.78uncharacterized calcium-binding protein At1g02270 isoform X2 [Momordica charanti... [more]
XP_022141210.10.096.05uncharacterized calcium-binding protein At1g02270 isoform X3 [Momordica charanti... [more]
XP_022923111.10.095.39uncharacterized calcium-binding protein At1g02270 isoform X1 [Cucurbita moschata... [more]
XP_022984360.10.095.39uncharacterized calcium-binding protein At1g02270 isoform X1 [Cucurbita maxima] ... [more]
Match NameE-valueIdentityDescription
A0A6J1CJT70.0100.00uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Momordica charan... [more]
A0A6J1CHY00.099.78uncharacterized calcium-binding protein At1g02270 isoform X2 OS=Momordica charan... [more]
A0A6J1CHC30.096.05uncharacterized calcium-binding protein At1g02270 isoform X3 OS=Momordica charan... [more]
A0A6J1E5X50.095.39uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Cucurbita moscha... [more]
A0A6J1J8E90.095.39uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT5G54130.21.8e-21479.82Calcium-binding endonuclease/exonuclease/phosphatase family [more]
AT1G02270.11.6e-17066.83Calcium-binding endonuclease/exonuclease/phosphatase family [more]
AT5G54130.41.2e-16281.38Calcium-binding endonuclease/exonuclease/phosphatase family [more]
AT5G54130.39.8e-16080.78Calcium-binding endonuclease/exonuclease/phosphatase family [more]
AT3G18500.24.9e-1028.81DNAse I-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 311..389
e-value: 1.2E-7
score: 33.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 381..407
NoneNo IPR availablePANTHERPTHR12121:SF87ENDONUCLEASE/EXONUCLEASE/PHOSPHATASE FAMILY PROTEINcoord: 9..452
NoneNo IPR availablePANTHERPTHR12121CARBON CATABOLITE REPRESSOR PROTEIN 4coord: 9..452
IPR005135Endonuclease/exonuclease/phosphatasePFAMPF03372Exo_endo_phoscoord: 41..249
e-value: 3.6E-11
score: 43.1
IPR036691Endonuclease/exonuclease/phosphatase superfamilyGENE3D3.60.10.10Endonuclease/exonuclease/phosphatasecoord: 20..310
e-value: 1.8E-39
score: 137.7
IPR036691Endonuclease/exonuclease/phosphatase superfamilySUPERFAMILY56219DNase I-likecoord: 35..252
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 363..375
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 237..382

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC10g0013MC10g0013gene


The following utr5 feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC10g0013.1-utr5MC10g0013.1-utr5-MC10:108807..108850utr5
MC10g0013.1-utr5MC10g0013.1-utr5-MC10:109387..109415utr5


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC10g0013.1-cdsMC10g0013.1-cds-MC10:109416..109580CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:109660..109764CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:109881..109986CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:110237..110457CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:110548..110624CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:110720..110828CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:110943..111068CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:111517..111627CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:111749..111856CDS
MC10g0013.1-cdsMC10g0013.1-cds-MC10:111965..112207CDS


The following utr3 feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC10g0013.1-utr3MC10g0013.1-utr3-MC10:112208..112983utr3


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC10g0013.1MC10g0013.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
molecular_function GO:0003824 catalytic activity
molecular_function GO:0000175 3'-5'-exoribonuclease activity
molecular_function GO:0005509 calcium ion binding