MC08g_new0419.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0419.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Receptor-like protein kinase
Location	MC08: 21039448 .. 21042624 (-)
Sequence length	474
RNA-Seq Expression	MC08g_new0419.1
Synteny	MC08g_new0419.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTCATAAAAAATCTAAAAAATAACTCATTTCCTTTGTAAAACATATTTTGCCTTATCATTCCCAACTGTATAGATTAATGTGTGAAGAAAGAAATGCGCAGTGGAAAATTGTGCTCTATAACTCTGGCTATAAACAAGGGCCAAATTTCCCCAATTCCCAAAATGTTTTCCACCATGAATTTCCCCAATTACATAATCCTAATTTTCATCCTCCATTACTTTCCATTTCTCCAATATCTCCCCCATTCTCAAGGCCTCCATTTTAATAACATCACTGATCAGGAAGCTCTCATTTCATTCAAATTCTCAACTTCTGACCCACATGGCGCCTTGAGTTCTTGGCTTCCAAACACTTCTTTCTGCTTCTGGCATGGCGTTCTCTGCAACCCAATTAAGCGCCGAGTAACCGGTCTCCAACTCGGGAGTCACTCACTCGCGGGACCCATTTCCCCCCACCTCACCAACCTCTCCTTCCTCCGCCACCTCGACCTGCAAAGCAATAACTTCTCCGGCACAATTCCGATGAAGATTCATCGTATTTTCCGCTTACGATTTTTGGACTTGAGTTCCAACAGTCTTCACGGAGCCATTCCCCAATCTTTGAGCCGCTGTTCTCAGCTTCGAACCATTAATTTGTATGATAACAAACTTCATGGTACCATTCCTTCTGAATTGGGTACCTTATCAGAGCTTCAAAATTTGAATCTTGGCCAGAATAACCTCTCTGGACCCATCCCTTCTTCATTTGGTAATCTTTCTTCCCTCGAGCAGTTGAATTTAATTACCAATAATTTAGAGGGTCCAATCCCGCTTGAATTAGCCCGTCTCAATCGGCTCTGGTATTTGCAGTTAGGAGACAACAAAATCTCAGGCAGTTTTCCCACCCACCTCATGAATATTTCTTCCTTGAATTCGTTTTCTTTGACCAAGAATCAGATTTCTGGTAAATTTCCATCTAATTTGTTCACTGTACTGAGTAATCTCTCCATTGCGTTTTTTGGAGGAAATCAGTTCACCGGCTCAATCCCAGAATCTCTTTCAAATGCCTCCACCGTCGAAAAACTTGACCTCTCAACTAACCAATTTTCTGGAGAAATTCCTTCACTTTGGAAGCTACAAATGATCCAATATCTGAACCTTGAGATCAATTATCTAACAAGTGAAGGAAAGGAAGGCCTTAACTTCATCACTTCACTTACCAATTAACTCACCTGAAAGTGTTGTCTGTGGCCACTAATTTACTCACTGGTCAGCTTCCACCCTCCATTGGAAACTTGTCCAACCAAATTTTTAAACTTCTCATGGCAGAAAACCAGCTCAATGGAAGCATTCCTGGAGAAATTGGCAATTTGGGTGGTCTCATCTCTCTATCCTTACCTCTCAATTCCTTCACAGGGAACATTCCTTCTTCATTTGGAAACCTCAAGAACCTGCAGTTTCTATCTTTAGAATCAAATTTTCTTTCGGGATCCATCCCTGAAACACTTGGGAATCTAAGCCAACTCTCCCTGTTTGCAGTTAATGATAACAACTTGAGTGGTGAAATCCCTCTAAGTTTAACAAGTTGGGAGCGTCTCCTCCTTCTAGATCTATCTCAAAATGGGTTCATTGGGAACATACCGAATGAAATCTCTAGATTCACAACTCTGTCGTTCTTCATGTTTCATTCAACAAGTTTACAGGGTCATTGCCTTCTGAAATTGGCAAGTTATCAACGGTTGAGATTTTGGATGTCTCCAGAAACCAACTCTCCGGACACATTCCAGCTACAATAGGCAGCTGCTTGAGTCTTGAAGACTTGAACATGTCTGGAAATTCTTTCCAAGGCCCAATTCCGAGTTCATTTGCCAATCTGAGAGGGATAAATTCCATAGACCTTTCATCAAACAGGTTGTCTGCAAAAATCCCTTCCCTCGATGGTCTTCGATACCTCCAACACTTGAACCTCTCTTCCAACAAACTACAAGGAGAAGTACCCAGAAGTGGCATCTTTCTCAACATTTCCAAAGTATTTTTATCCAACAATCCCGAGCTCTGCGGCGGCGTACTTGAATTTGGACTGCCAAAATGCCCTACCAGCTCAATTGGCAAGAGAAAAAATGGGAAATTAATCGCGGGAATTGTTGGAGGAGCAACTGGTTTGTGTATTGCAATTGCTTTGGGTTTTGTCTTGCGCATGAAGAGACGGAAACGGGTGGTGATTTCATTTGAAGGGCTGGATCATAGACTATATTCCTACTATGAACTAAAACATGCCACATGAGATTTCACCAATGAAAATCTGATCGGAAAGGGGAGCTTTGGATCTGTTTATAAAGGTGTAATGCGAGACGGTACTTGGATCGCCATTAAAGTGATCGACTTGGATCATCGTTGTGGAATGAAGGGCTTTCTTACCGAATGTGAAATCTTCAGAAACCTGAGGCATCGAAACCTCGTGAAGATCCTCAGTGCTTGTTCTACCATGGAATTCAAGGCACTAGTTTTTGAATTCATGGCGAACGGGAACTTGGAAACTTGGCTTCACAGAAGATCAGAGCGTTGGCTAACTCTAAAGCAAAGAATAGACATAGCTTTAGATGTTGGAGCTGCAATGGAGTATCTTCACCATGGATTGGAAACTCCTGTAGTTCACTGCGATCTTAAGCCCAGCAATGTTCTTTTAGATGAAGAAATGTGGGCCCATGTTGCAGATTTTGGGCTTGCAAAATTGCTTCAACCACAAGCCCATTCTGGAGACATCACTAGTGGGCTAAAAGGATCTATCGGCTACATTGCACCAGGTTCTATCTTTTTTCTGAAATTTCATTACCTTGAGATGGAGGGTAGAATGAGTAATTTTAAAAAACGAGATTTAATTATAATTGATTTAACGTCTAAATGATACTAAAAAATAAATTTAGAGTGATTTTAGGTATGTTTGGGGGTGATGGTAGAATAAGTGATTTTGGAAAAAAATATTTAAGTATAATTAGTTTTCCAAAATCATCATCCAAGTGATACCTAAGAATCAATTTTAAGTGATTTTTAAGTTTTCATAAAATCAATTTTCTAATTTGCAAAACATAATCAATGGAGGAGTGATTTTGACAGTAGCAAAAGTGATTTTGGCCATCCAAACACACTCTTAAACTTTTCTAAAATCAATTTTTTACTTTGCCAAACATTATTGAAG

mRNA sequence

ATGGCAGAAAACCAGCTCAATGGAAGCATTCCTGGAGAAATTGGCAATTTGGGTGGTCTCATCTCTCTATCCTTACCTCTCAATTCCTTCACAGGGAACATTCCTTCTTCATTTGGAAACCTCAAGAACCTGCAGTTTCTATCTTTAGAATCAAATTTTCTTTCGGGATCCATCCCTGAAACACTTGGGAATCTAAGCCAACTCTCCCTGTTTGCAGTTAATGATAACAACTTGAGTGGTGAAATCCCTCTAAGTTTAACAAGTTGGGAGCGTCTCCTCCTTCTAGATCTATCTCAAAATGGGTTCATTGGGAACATACCGAATGAAATCTCTAGATTCACAACTCTGTCGTTCTTCATGTTTCATTCAACAAGTTTACAGGGTCATTGCCTTCTGAAATTGGCAAGTTATCAACGGTTGAGATTTTGGATGTCTCCAGAAACCAACTCTCCGGACACATTCCAGCTACAATAG

Coding sequence (CDS)

ATGGCAGAAAACCAGCTCAATGGAAGCATTCCTGGAGAAATTGGCAATTTGGGTGGTCTCATCTCTCTATCCTTACCTCTCAATTCCTTCACAGGGAACATTCCTTCTTCATTTGGAAACCTCAAGAACCTGCAGTTTCTATCTTTAGAATCAAATTTTCTTTCGGGATCCATCCCTGAAACACTTGGGAATCTAAGCCAACTCTCCCTGTTTGCAGTTAATGATAACAACTTGAGTGGTGAAATCCCTCTAAGTTTAACAAGTTGGGAGCGTCTCCTCCTTCTAGATCTATCTCAAAATGGGTTCATTGGGAACATACCGAATGAAATCTCTAGATTCACAACTCTGTCGTTCTTCATGTTTCATTCAACAAGTTTACAGGGTCATTGCCTTCTGAAATTGGCAAGTTATCAACGGTTGAGATTTTGGATGTCTCCAGAAACCAACTCTCCGGACACATTCCAGCTACAATAG

Protein sequence

MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPETLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFMFHSTSLQGHCLLKLASYQRLRFWMSPETNSPDTFQLQ

Homology

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy Swiss-Prot
Match: Q9SRL7 (Receptor-like protein 35 OS=Arabidopsis thaliana OX=3702 GN=RLP35 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.8e-23
Identity = 60/128 (46.88%), Postives = 74/128 (57.81%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           ++ NQ +G IP  IGNL  L  LSLP N F G IPSS GNL  L +L L  N   G IP 
Sbjct: 204 LSSNQFSGQIPSSIGNLSNLTFLSLPSNDFFGQIPSSIGNLARLTYLYLSYNNFVGEIPS 263

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           + GNL+QL +  V+ N LSG +P+SL +  RL  L LS N F G IPN IS  + L  F 
Sbjct: 264 SFGNLNQLIVLQVDSNKLSGNVPISLLNLTRLSALLLSHNQFTGTIPNNISLLSNLMDFE 323

Query: 121 FHSTSLQG 129
             + +  G
Sbjct: 324 ASNNAFTG 331

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy Swiss-Prot
Match: C0LGF5 (LRR receptor-like serine/threonine-protein kinase RGI5 OS=Arabidopsis thaliana OX=3702 GN=RGI5 PE=1 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-21
Identity = 61/126 (48.41%), Postives = 75/126 (59.52%), Query Frame = 0


Query: 4   NQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPETLG 63
           N + G IP ++GNL  L  L L  NSFTGNIP SFGNL  L  L L +N L+G IP+++ 
Sbjct: 510 NYITGDIPAQLGNLVNLEQLDLSRNSFTGNIPLSFGNLSYLNKLILNNNLLTGQIPKSIK 569

Query: 64  NLSQLSLFAVNDNNLSGEIPLSLTSWERLLL-LDLSQNGFIGNIPNEISRFTTLSFFMFH 123
           NL +L+L  ++ N+LSGEIP  L     L + LDLS N F GNIP   S  T L      
Sbjct: 570 NLQKLTLLDLSYNSLSGEIPQELGQVTSLTINLDLSYNTFTGNIPETFSDLTQLQSLDLS 629

Query: 124 STSLQG 129
           S SL G
Sbjct: 630 SNSLHG 635

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy Swiss-Prot
Match: Q9LHP4 (LRR receptor-like serine/threonine-protein kinase RGI1 OS=Arabidopsis thaliana OX=3702 GN=RGI1 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.6e-21
Identity = 56/126 (44.44%), Postives = 73/126 (57.94%), Query Frame = 0


Query: 3   ENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPETL 62
           EN L+GSIP EIG L  L  L L  NS  G IP   GN  NL+ + L  N LSGSIP ++
Sbjct: 283 ENSLSGSIPREIGQLTKLEQLFLWQNSLVGGIPEEIGNCSNLKMIDLSLNLLSGSIPSSI 342

Query: 63  GNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFMFH 122
           G LS L  F ++DN  SG IP ++++   L+ L L +N   G IP+E+   T L+ F   
Sbjct: 343 GRLSFLEEFMISDNKFSGSIPTTISNCSSLVQLQLDKNQISGLIPSELGTLTKLTLFFAW 402

Query: 123 STSLQG 129
           S  L+G
Sbjct: 403 SNQLEG 408

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy Swiss-Prot
Match: Q9ZUK7 (Receptor-like protein 18 OS=Arabidopsis thaliana OX=3702 GN=RLP18 PE=2 SV=3)

HSP 1 Score: 102.1 bits (253), Expect = 6.0e-21
Identity = 59/140 (42.14%), Postives = 79/140 (56.43%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           ++EN  +G IP  +GNL  L SL L  N+F G+IP+S GNL +L  L L +N L G IP 
Sbjct: 63  LSENHFSGQIPSSLGNLLHLTSLDLTDNNFVGDIPTSLGNLSHLTLLLLGANNLVGEIPF 122

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           +LGNLS L+   + +N+L+GEIP S  +   L  LDLSQN  +G IP+    F  L    
Sbjct: 123 SLGNLSHLTDLTLCENDLAGEIPSSFENLSHLTNLDLSQNNLVGEIPSFFGSFNQLVSLA 182

Query: 121 FHSTSLQGHCLLKLASYQRL 141
                  G+ LL L +   L
Sbjct: 183 VEENEFTGNFLLILLNLTNL 202

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy Swiss-Prot
Match: Q8VZG8 (MDIS1-interacting receptor like kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MIK2 PE=1 SV=3)

HSP 1 Score: 101.7 bits (252), Expect = 7.9e-21
Identity = 57/128 (44.53%), Postives = 75/128 (58.59%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           + EN+LNGSIP EIG L  +  +++  N  TG IPSSFGNL  L  L L  N LSGSIP 
Sbjct: 173 LVENKLNGSIPSEIGRLTKVTEIAIYDNLLTGPIPSSFGNLTKLVNLYLFINSLSGSIPS 232

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
            +GNL  L    ++ NNL+G+IP S  + + + LL++ +N   G IP EI   T L    
Sbjct: 233 EIGNLPNLRELCLDRNNLTGKIPSSFGNLKNVTLLNMFENQLSGEIPPEIGNMTALDTLS 292

Query: 121 FHSTSLQG 129
            H+  L G
Sbjct: 293 LHTNKLTG 300

[Close]

BLAST of MC08g_new0419.1 vs. NCBI nr
Match: XP_022960963.1 (LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita moschata])

HSP 1 Score: 178 bits (452), Expect = 1.61e-48
Identity = 93/128 (72.66%), Postives = 101/128 (78.91%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           MAENQ +GSIP EIGNLGGLIS+SLP NSFTGNIPSS  NLKNLQ LSLESN LSGSIPE
Sbjct: 376 MAENQFSGSIPSEIGNLGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPE 435

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           T GNLS+LSLF VNDN LSG+IPLSLT+ ERLL+ DLSQNG  GN+P EI  F  L  F 
Sbjct: 436 TFGNLSELSLFLVNDNKLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFN 495

Query: 121 FHSTSLQG 128
               +L G
Sbjct: 496 VSVNNLTG 503

[Close]

BLAST of MC08g_new0419.1 vs. NCBI nr
Match: XP_022971300.1 (LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita maxima])

HSP 1 Score: 178 bits (452), Expect = 1.61e-48
Identity = 93/128 (72.66%), Postives = 101/128 (78.91%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           MAENQ +GSIP EIGNLGGLIS+SLP NSFTGNIPSS  NLKNLQ LSLESN LSGSIPE
Sbjct: 376 MAENQFSGSIPSEIGNLGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPE 435

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           T GNLS+LSLF VNDN LSG+IPLSLT+ ERLL+ DLSQNG  GN+P EI  F  L  F 
Sbjct: 436 TFGNLSELSLFLVNDNKLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFN 495

Query: 121 FHSTSLQG 128
               +L G
Sbjct: 496 VSVNNLTG 503

[Close]

BLAST of MC08g_new0419.1 vs. NCBI nr
Match: KAG6596398.1 (LRR receptor-like serine/threonine-protein kinase EFR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 176 bits (445), Expect = 1.42e-47
Identity = 92/128 (71.88%), Postives = 100/128 (78.12%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           MAENQ +GSIP EIGNLGGLIS+SLP NSFTGNIPSS  NLKNLQ LSLESN LSGSIPE
Sbjct: 376 MAENQFSGSIPSEIGNLGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPE 435

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           T GNLS+LSLF VNDN LSG+IPLSLT+ ERL + DLSQNG  GN+P EI  F  L  F 
Sbjct: 436 TFGNLSELSLFLVNDNKLSGKIPLSLTNCERLHVFDLSQNGLSGNLPKEIFSFPNLLEFN 495

Query: 121 FHSTSLQG 128
               +L G
Sbjct: 496 VSVNNLTG 503

[Close]

BLAST of MC08g_new0419.1 vs. NCBI nr
Match: XP_023539541.1 (LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita pepo subsp. pepo])

HSP 1 Score: 172 bits (435), Expect = 2.70e-46
Identity = 90/128 (70.31%), Postives = 99/128 (77.34%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           MAENQ +GSIP EIGNLGGLIS+SLP NSF GNIPSS  NLKNLQ LSLESN LSGSIPE
Sbjct: 376 MAENQFSGSIPSEIGNLGGLISVSLPSNSFMGNIPSSLSNLKNLQVLSLESNILSGSIPE 435

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
             GNLS+LSLF V+DN LSG+IPLSLT+ ERLL+ DLSQNG  GN+P EI  F  L  F 
Sbjct: 436 PFGNLSELSLFLVSDNKLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFN 495

Query: 121 FHSTSLQG 128
               +L G
Sbjct: 496 VSVNNLTG 503

[Close]

BLAST of MC08g_new0419.1 vs. NCBI nr
Match: KAE8651004.1 (hypothetical protein Csa_002076 [Cucumis sativus])

HSP 1 Score: 154 bits (390), Expect = 2.30e-44
Identity = 80/129 (62.02%), Postives = 94/129 (72.87%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           +AENQ +G+IP EIGNL GLIS+S   NSFTGNIP    NLKNLQ LSL+SNFLSG IPE
Sbjct: 46  IAENQFSGTIPEEIGNLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPE 105

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           T GNLS+ S F VNDNNL G+IP SLT+ ERL +  +S+NG IGN+P+EI  F TL  F 
Sbjct: 106 TFGNLSEPSQFTVNDNNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFN 165

Query: 121 FHSTSLQGH 129
               +  GH
Sbjct: 166 VSFNNFTGH 174

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy TrEMBL
Match: A0A6J1I5C7 (LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita maxima OX=3661 GN=LOC111470057 PE=3 SV=1)

HSP 1 Score: 178 bits (452), Expect = 7.80e-49
Identity = 93/128 (72.66%), Postives = 101/128 (78.91%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           MAENQ +GSIP EIGNLGGLIS+SLP NSFTGNIPSS  NLKNLQ LSLESN LSGSIPE
Sbjct: 376 MAENQFSGSIPSEIGNLGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPE 435

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           T GNLS+LSLF VNDN LSG+IPLSLT+ ERLL+ DLSQNG  GN+P EI  F  L  F 
Sbjct: 436 TFGNLSELSLFLVNDNKLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFN 495

Query: 121 FHSTSLQG 128
               +L G
Sbjct: 496 VSVNNLTG 503

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy TrEMBL
Match: A0A6J1HAI5 (LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita moschata OX=3662 GN=LOC111461581 PE=3 SV=1)

HSP 1 Score: 178 bits (452), Expect = 7.80e-49
Identity = 93/128 (72.66%), Postives = 101/128 (78.91%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           MAENQ +GSIP EIGNLGGLIS+SLP NSFTGNIPSS  NLKNLQ LSLESN LSGSIPE
Sbjct: 376 MAENQFSGSIPSEIGNLGGLISVSLPSNSFTGNIPSSLSNLKNLQVLSLESNILSGSIPE 435

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           T GNLS+LSLF VNDN LSG+IPLSLT+ ERLL+ DLSQNG  GN+P EI  F  L  F 
Sbjct: 436 TFGNLSELSLFLVNDNKLSGKIPLSLTNCERLLVFDLSQNGLSGNLPKEIFSFPNLLEFN 495

Query: 121 FHSTSLQG 128
               +L G
Sbjct: 496 VSVNNLTG 503

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy TrEMBL
Match: A0A0A0LAH4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734020 PE=4 SV=1)

HSP 1 Score: 154 bits (390), Expect = 2.50e-43
Identity = 80/129 (62.02%), Postives = 94/129 (72.87%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           +AENQ +G+IP EIGNL GLIS+S   NSFTGNIP    NLKNLQ LSL+SNFLSG IPE
Sbjct: 46  IAENQFSGTIPEEIGNLVGLISVSFLTNSFTGNIPFPLENLKNLQILSLKSNFLSGFIPE 105

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           T GNLS+ S F VNDNNL G+IP SLT+ ERL +  +S+NG IGN+P+EI  F TL  F 
Sbjct: 106 TFGNLSEPSQFTVNDNNLGGKIPPSLTNCERLTIFYISENGLIGNLPDEIFNFKTLLIFN 165

Query: 121 FHSTSLQGH 129
               +  GH
Sbjct: 166 VSFNNFTGH 174

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy TrEMBL
Match: A0A6J1CXN3 (uncharacterized protein LOC111015147 OS=Momordica charantia OX=3673 GN=LOC111015147 PE=3 SV=1)

HSP 1 Score: 140 bits (352), Expect = 2.58e-35
Identity = 72/117 (61.54%), Postives = 92/117 (78.63%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           ++ENQL+G++P EIGNLGGL  L+L  NSFTG IPSS GNL++LQ L L +NFLSGSIPE
Sbjct: 374 LSENQLSGNLPQEIGNLGGLNVLTLDSNSFTGKIPSSLGNLRDLQALYLYTNFLSGSIPE 433

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEI-SRFTTL 116
           +LGNLS LS   +N+NNLSG IPLS ++ +R+ + D+S NG  G+IP EI S +TTL
Sbjct: 434 SLGNLSSLSEIGLNENNLSGGIPLSFSNCKRIKVFDVSANGLSGSIPKEIFSAYTTL 490

[Close]

BLAST of MC08g_new0419.1 vs. ExPASy TrEMBL
Match: A0A438DE81 (LRR receptor-like serine/threonine-protein kinase EFR OS=Vitis vinifera OX=29760 GN=EFR_18 PE=4 SV=1)

HSP 1 Score: 126 bits (317), Expect = 1.24e-30
Identity = 65/117 (55.56%), Postives = 83/117 (70.94%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           M +N   G+IP  +GNL  LI LS+  N  TG+IPS+ GNL+NLQ L L+SN+LSGSIPE
Sbjct: 305 MGQNHFEGNIPEGVGNLRSLIQLSMEENVLTGHIPSTIGNLQNLQSLILDSNYLSGSIPE 364

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLS 117
           +LGNL+QL    ++ NN++G IP SL+S +RL LLDLS NG   NIP EI  F  L+
Sbjct: 365 SLGNLTQLYELGLSGNNITGRIPSSLSSCQRLQLLDLSINGLRDNIPKEIFSFPNLA 421

[Close]

BLAST of MC08g_new0419.1 vs. TAIR 10
Match: AT3G11080.1 (receptor like protein 35 )

HSP 1 Score: 109.4 bits (272), Expect = 2.7e-24
Identity = 60/128 (46.88%), Postives = 74/128 (57.81%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           ++ NQ +G IP  IGNL  L  LSLP N F G IPSS GNL  L +L L  N   G IP 
Sbjct: 204 LSSNQFSGQIPSSIGNLSNLTFLSLPSNDFFGQIPSSIGNLARLTYLYLSYNNFVGEIPS 263

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
           + GNL+QL +  V+ N LSG +P+SL +  RL  L LS N F G IPN IS  + L  F 
Sbjct: 264 SFGNLNQLIVLQVDSNKLSGNVPISLLNLTRLSALLLSHNQFTGTIPNNISLLSNLMDFE 323

Query: 121 FHSTSLQG 129
             + +  G
Sbjct: 324 ASNNAFTG 331

[Close]

BLAST of MC08g_new0419.1 vs. TAIR 10
Match: AT1G34110.1 (Leucine-rich receptor-like protein kinase family protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-22
Identity = 61/126 (48.41%), Postives = 75/126 (59.52%), Query Frame = 0


Query: 4   NQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPETLG 63
           N + G IP ++GNL  L  L L  NSFTGNIP SFGNL  L  L L +N L+G IP+++ 
Sbjct: 510 NYITGDIPAQLGNLVNLEQLDLSRNSFTGNIPLSFGNLSYLNKLILNNNLLTGQIPKSIK 569

Query: 64  NLSQLSLFAVNDNNLSGEIPLSLTSWERLLL-LDLSQNGFIGNIPNEISRFTTLSFFMFH 123
           NL +L+L  ++ N+LSGEIP  L     L + LDLS N F GNIP   S  T L      
Sbjct: 570 NLQKLTLLDLSYNSLSGEIPQELGQVTSLTINLDLSYNTFTGNIPETFSDLTQLQSLDLS 629

Query: 124 STSLQG 129
           S SL G
Sbjct: 630 SNSLHG 635

[Close]

BLAST of MC08g_new0419.1 vs. TAIR 10
Match: AT3G24240.1 (Leucine-rich repeat receptor-like protein kinase family protein )

HSP 1 Score: 102.4 bits (254), Expect = 3.3e-22
Identity = 56/126 (44.44%), Postives = 73/126 (57.94%), Query Frame = 0


Query: 3   ENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPETL 62
           EN L+GSIP EIG L  L  L L  NS  G IP   GN  NL+ + L  N LSGSIP ++
Sbjct: 283 ENSLSGSIPREIGQLTKLEQLFLWQNSLVGGIPEEIGNCSNLKMIDLSLNLLSGSIPSSI 342

Query: 63  GNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFMFH 122
           G LS L  F ++DN  SG IP ++++   L+ L L +N   G IP+E+   T L+ F   
Sbjct: 343 GRLSFLEEFMISDNKFSGSIPTTISNCSSLVQLQLDKNQISGLIPSELGTLTKLTLFFAW 402

Query: 123 STSLQG 129
           S  L+G
Sbjct: 403 SNQLEG 408

[Close]

BLAST of MC08g_new0419.1 vs. TAIR 10
Match: AT4G08850.1 (Leucine-rich repeat receptor-like protein kinase family protein )

HSP 1 Score: 101.7 bits (252), Expect = 5.6e-22
Identity = 57/128 (44.53%), Postives = 75/128 (58.59%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           + EN+LNGSIP EIG L  +  +++  N  TG IPSSFGNL  L  L L  N LSGSIP 
Sbjct: 173 LVENKLNGSIPSEIGRLTKVTEIAIYDNLLTGPIPSSFGNLTKLVNLYLFINSLSGSIPS 232

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
            +GNL  L    ++ NNL+G+IP S  + + + LL++ +N   G IP EI   T L    
Sbjct: 233 EIGNLPNLRELCLDRNNLTGKIPSSFGNLKNVTLLNMFENQLSGEIPPEIGNMTALDTLS 292

Query: 121 FHSTSLQG 129
            H+  L G
Sbjct: 293 LHTNKLTG 300

[Close]

BLAST of MC08g_new0419.1 vs. TAIR 10
Match: AT4G08850.2 (Leucine-rich repeat receptor-like protein kinase family protein )

HSP 1 Score: 101.7 bits (252), Expect = 5.6e-22
Identity = 57/128 (44.53%), Postives = 75/128 (58.59%), Query Frame = 0


Query: 1   MAENQLNGSIPGEIGNLGGLISLSLPLNSFTGNIPSSFGNLKNLQFLSLESNFLSGSIPE 60
           + EN+LNGSIP EIG L  +  +++  N  TG IPSSFGNL  L  L L  N LSGSIP 
Sbjct: 173 LVENKLNGSIPSEIGRLTKVTEIAIYDNLLTGPIPSSFGNLTKLVNLYLFINSLSGSIPS 232

Query: 61  TLGNLSQLSLFAVNDNNLSGEIPLSLTSWERLLLLDLSQNGFIGNIPNEISRFTTLSFFM 120
            +GNL  L    ++ NNL+G+IP S  + + + LL++ +N   G IP EI   T L    
Sbjct: 233 EIGNLPNLRELCLDRNNLTGKIPSSFGNLKNVTLLNMFENQLSGEIPPEIGNMTALDTLS 292

Query: 121 FHSTSLQG 129
            H+  L G
Sbjct: 293 LHTNKLTG 300

The following BLAST results are available for this feature:

BLAST of MC08g_new0419.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRL7	3.8e-23	46.88	Receptor-like protein 35 OS=Arabidopsis thaliana OX=3702 GN=RLP35 PE=3 SV=1	[more]
C0LGF5	1.6e-21	48.41	LRR receptor-like serine/threonine-protein kinase RGI5 OS=Arabidopsis thaliana O...	[more]
Q9LHP4	4.6e-21	44.44	LRR receptor-like serine/threonine-protein kinase RGI1 OS=Arabidopsis thaliana O...	[more]
Q9ZUK7	6.0e-21	42.14	Receptor-like protein 18 OS=Arabidopsis thaliana OX=3702 GN=RLP18 PE=2 SV=3	[more]
Q8VZG8	7.9e-21	44.53	MDIS1-interacting receptor like kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MIK2...	[more]

BLAST of MC08g_new0419.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960963.1	1.61e-48	72.66	LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita moschata]	[more]
XP_022971300.1	1.61e-48	72.66	LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita maxima]	[more]
KAG6596398.1	1.42e-47	71.88	LRR receptor-like serine/threonine-protein kinase EFR, partial [Cucurbita argyro...	[more]
XP_023539541.1	2.70e-46	70.31	LRR receptor-like serine/threonine-protein kinase EFR [Cucurbita pepo subsp. pep...	[more]
KAE8651004.1	2.30e-44	62.02	hypothetical protein Csa_002076 [Cucumis sativus]	[more]

BLAST of MC08g_new0419.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I5C7	7.80e-49	72.66	LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita maxima OX=366...	[more]
A0A6J1HAI5	7.80e-49	72.66	LRR receptor-like serine/threonine-protein kinase EFR OS=Cucurbita moschata OX=3...	[more]
A0A0A0LAH4	2.50e-43	62.02	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G734020 PE=4 SV=1	[more]
A0A6J1CXN3	2.58e-35	61.54	uncharacterized protein LOC111015147 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]
A0A438DE81	1.24e-30	55.56	LRR receptor-like serine/threonine-protein kinase EFR OS=Vitis vinifera OX=29760...	[more]

BLAST of MC08g_new0419.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11080.1	2.7e-24	46.88	receptor like protein 35	[more]
AT1G34110.1	1.1e-22	48.41	Leucine-rich receptor-like protein kinase family protein	[more]
AT3G24240.1	3.3e-22	44.44	Leucine-rich repeat receptor-like protein kinase family protein	[more]
AT4G08850.1	5.6e-22	44.53	Leucine-rich repeat receptor-like protein kinase family protein	[more]
AT4G08850.2	5.6e-22	44.53	Leucine-rich repeat receptor-like protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 1..150 e-value: 3.0E-34 score: 120.2
None	No IPR available	PANTHER	PTHR46662:SF19	OS06G0656632 PROTEIN	coord: 23..92
None	No IPR available	PANTHER	PTHR46662	DI-GLUCOSE BINDING PROTEIN WITH LEUCINE-RICH REPEAT DOMAIN-CONTAINING PROTEIN	coord: 23..92 coord: 75..141
None	No IPR available	PANTHER	PTHR46662:SF19	OS06G0656632 PROTEIN	coord: 75..141
None	No IPR available	PANTHER	PTHR46662	DI-GLUCOSE BINDING PROTEIN WITH LEUCINE-RICH REPEAT DOMAIN-CONTAINING PROTEIN	coord: 2..69
None	No IPR available	PANTHER	PTHR46662:SF19	OS06G0656632 PROTEIN	coord: 2..69
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 3..143

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC08g_new0419	MC08g_new0419	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g_new0419.1-cds	MC08g_new0419.1-cds-MC08:21040848..21041321	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC08g_new0419.1	MC08g_new0419.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity