MC08g1398.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1398.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	aquaporin NIP2-1-like
Location	MC08: 14723573 .. 14728882 (-)
Sequence length	1334
RNA-Seq Expression	MC08g1398.1
Synteny	MC08g1398.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTCTAATGGCGAGAGGTTGAATTAAAAATTATATATATTTTTATAATAGATTCATAATACAAAGAGACACTGATGTGTAACTACTTGAACCGTACACCCATCCTCCATTTCACTATAAAATGGGTCGCTTGGTTCCACCTATTGATGTGCAGAGAGAGGCTTTTTTTGTTTGAGTATATATTAATATTTTAGGTTGAAAAATGGGTTCGGTTCATGATCCGGAGGCCGGCGACGGTTACCGCAGCGGCGGCGCTGCCACCGCCGACGTGGAGGAGTTCGTGTCAGTAGAAAACCCAGACTCCAAACTCTCCAAGTTTGGATCTATCTTCCAAAACCTTTACCCTCCTGGGTTTTCCCGAAAGGTACCTTAAAATCATCAAACAAACAATTGACCCTTTTCCTTTGTCCTTTTGTTTTTATTTTTTTTGGGAAAAATCATTCCCTGGTGGAGTAGAATGAAAATTTCTATCTCCTGTCGCTGACGAAATTTTTAGAATTGAAAGATTATATACATAAAAGTGAAAATTTATGGCTTTGCCTCTTGATATGGTGTTCAGCTGGTGGCGGAAGTGATAGCGACATATTTGCTGGTGTTTGTGACGTGCGGGGCGGGGGCATTGAGCGCGAGCGACGAGCGGAGAGTGTCGCAGCTCGGAGCCTCAGTCGCGGGCGGGCTGATCGTCACCGTGATGATCTACGCTGTCGGCCACATATCGGGCGCCCACATGAACCCGGCTGTCACCTTAGCTTTCGCTGCAACTCGACACTTTCCATGGAAACAGGTCTCAGTCCATACACTTTTTTTTTCTTTCCCTTTTGATGCAATTCTCAGTTATTTCTGTTATAATCAATAACCTAATCATTTGCTCTCACATGCTATCTTGTCGGTTTTATTATCCAGTAACATTCGTGTTTTCTTTTTTCCTTTATCGTGACCCATTTTTTAGATCTTGTTTGATTCCTAAATATTTTATTTTATTTTATTTGATACTTGTACTTCAATTTTTTTTTTCATTGAAATTTATTATCCATCTCTTTAAATGTTGCATAAAAAATATTTCTCATTTGCATGATCTATAATCATCAATCTATGATTGACAAATGTTTGAAACCACATATATTAGATTAGTTGTTGGCGAGTCTTAATTTTTTTTCTTGTTCTTTTAAATAATTGATGAATCTTTTTAAGTAAAATTTGGAGATGAAAATCAATCACTTGAATGTTAAAGGACTAAAAATAAAATAAAAGAAATTTGAAGTACTAAATATAGGAGTTAAACTTAAATTTATTGAATAAAACTATATTATTTATTTATTTTACTAATATCGAAATGAATAATTATTTAATTCAAGTAAATAAATTATTAAATAATATGTCAATAATGATAAGAGCTGATTTTAATAGAGCTAGTTGAATAAAAAACATAATAATTTATAATAAATGAACACGTATCTTATGTTTTTACTGATAATATATACAAATGGTTTTTTAAATGAATATTATTTAATTATCAAATTGATGTGTTAGAATAATTATTTGATATAGTAGAGCCTATTTGTTTGAGGTTTATTATTAATATTCAGGTGCGGTGCCAAGTACAGAGGACCCGGCATCTATTTCCCATTAATTTTGAATTATATAAATTCAATTTCAAATATTTTTTCTCCTTGATTATCCCATACAAATTGAAATTTAAAATTTGTCCTCACTTTCAAGTTTTCCTGAACAACTTATTTTTACAGTTCAGTTTTCAAGTTGTTGTTTACTTTCATGCCTTTATAACAACTATTTTGTTTTTAATTTTTTAGTTTTTAAAAATTTTGTATAATTAAACACTTCCTTTTGTTTTGCATTTACTTTTAAAAACATTTTCAAAATCAAAGCTAAAATTTAAAAACTTAAAAAAGTAGTTTTCAAAAACTTAATTTTATTTTTAAGATGATGAAACATACCATTTTAAGATGGTGAAAACATATCAACGGGGATTGTAACCAAACAAGACTGATTTTCAAAAACAGAAAATTAAAAATGAAATAGTTATCAAACATGTCCTCACTTTCAAATCGTTACTAAAACATGATATTGAGTATGATTCTATGATTAAGTGATGGTCAATCTGAACCTAATTCAACTGTACCAACATCCAGTGTCTTTCCTATAAGTCATAAGCTCGAAGCAATCGAGTGAATAACACTATTTTGACTAATAATTTAAACATAAATTTTGTGGGGTTGAAAAATGAACCCTTTTTTCCATGTGTAGTTTTGAAGTCCTAGAAGTAGATCATTATATTCATGATATAGAGAGGAAATGGAATAATGGACATGATGACAATAAAAAAGGGAAAAATCTAGGCCATATTTAGTGGCCAACATGAGGTAGGGTCCAGCCCAAATATTATTCTTGAGACCTCACGCAGCCACAAGACTTGTGATTTTTCCATGGCCATGAGCCTTTGGCCTCACTCCGCCGGCCACAAGAATGGGATGGGACATGAGCCATATGATAGCTCCAGAACACTTGAGAGAGGGATAGAGATGTCCACCAAACATTTTCTTGGCCCATTGGGAGTGGGGGTAGTATAGGACAACATGATATAATAATTCATGTGTGTGTTTTACTGTTTAGAGTCAAAAGAGCAGTGGACGTCTTTTAACTTTGCTTTTAGAATTAAAGAATTTCAAAGTAGTGTTGATAACCATTTTGTTTTCGGGTTTTTCTTTTTGAAAGTGTTTCTTTCCCTCATGTTTTCCGTTGTTAGTGTTATATAGTTTTTTAAACATTTTCATCTCTAGGCCAAATTTTGTTTTCATTTTTTAAAGTTTCAAAAACTTATTTTTGTTCAAGAGAATTAGCTAAGAATTGAAATACGTTTTTAAGAGTGTTGAAAAACTTACTACTGATATTGTAAAAAAAAACAAACATAATTATTAAAAATAGAAAAATAAAAATGAAATAGTTGTTTGGATACTAAGTCGTTGACTTTTAAAGTCCGGTTTCATCTTTGTTAGGAGATGGTACTTTTTGGCTCATAACTTTCGCAAGTCTTTGTTTAAGAGTTTTTATATATTAGAGATAGAAACTGGGTTTATACAACATCTATTGGTTTCGTAGGTTTGTGATTTGTAAATGTAAGATTCAACTTCTGAAGTAGATTTCAAAAGTTTAACTTGGGTTTATGGAAACAGGCTCATGGAAATATCTACTTATACTCTTGTGAAATGTTTGGTTCACAATTTTAATATCTATAAATTTCCCTGACTTGAAATTAAGATTTTTGGGACATGAGTTCGACACCTAGATTAGAAACGTTAAGTTTAGGAATTTAAGTGGAAATCTTCGTAAACCTAGCTAGGTTTAAGAAAATTGTGTGGGAGATGAGTGAGTACTGAAATTTGTCCATCAAATTCTAAACTTAATAAACCTAAACAACAAACATAGGCTAGTTCCACATCTCAAACCAAACCCACACCCTAAACATCCTATATATCAAAGAGGTTCTAGTTTGTACATCTTACATTTCCACTATAAAGAAGAAGTATAATTTATTAAATACGTTGTATCAGTAAGGATATAGGGCCTTGTGATATTTAGATACTTGAGCGGATAGTAGATTTACACAGAAGAGGGCTTCTTTTTTTTTTTTCAAATAGTTAATAAAATTTGGTAATTAAAGAGTTAAAAGGATTGGTTTGGGTGGTGGCAGGTTCCAGCGTATGCAGCAGCGCAGCTGAGTGGAGCAACATCTGCGGCCTTTACGCTGCGAATATTGCTGCATCCAATCAAACACGTGGGCACAACGTCGCCATCCGGATCGGATTTTCAGGCATTGGTCATGGAGATAGTTGTTACATTCTCCATGATGTTCGTTACTTCAGCTGTCGCAACCGACACCAAAGCTGTAAGAATATAATTAATTCTATCCTTTCCTCTCACTTTCTTCATTGTTTCACATGTTCCACGATGATAAACTTACATGACATGTTTGGTCGAAATTTCTTGATTATCTTCTAAATTTCTGTTAACTCGTTTAAGTATCATGTAATAAAGTTTATTATTGTCAACCAATTTTAATATTTACCACTGAAAAAGTGATGTAATTCAAAAGTTTTAAACCATAGAGAATAGATTCTGTTTTTCTTCTTAGGAAAAATTGTTTTCCAAAATTGTATTTATGAAAAAATTAAGGACTAAAGCGCATAATCTTGTAAATGTTGTGAAATTACACTCCACATCATAAATATAATATGTATTTTAAGAAATGTCATAACTTTTCATTTTGTTTTTAATTATAGCATAAATGTGATTTTATTTTGAGTTTATTGCATTTAGCAAACTTGAGTATAGACAAAACAAATCGTGTGTTCAAATTTTTACTATATTTGAAACAAATAATAACTCCAAGTTTAAAAATTAAATAATATTAAATAAAAAAACTGATATACTTATCTCCAATGGTATATATGCGTAGAACAGAATATATCACATCCTAAATTAAAGAATATGTCTGACTAGTTGATGTGTAATTAAATTTTATTCATTTAACAAGTGGTGCTTGATTTTTGGCAGGTGGGAGAGCTAGCAGGTATGGCAGTTGGCTCAGCTGTGTGTATTACATCCATCTTAGCTGGGTAAGGAAACATATAACACATTTTTGGATTGGCTTTTTACCCATAAAAATATTTGATTTCTAATTAACATCTTGAAATAAAACAAATTTTGAAAAGTTTCTAATTTGTTTTACCAAAGAATTTTGAGCATACACATGAAATATTAAACAAATGAATCTAATGGAAGACTTGTGTTAGCCACTGTTCAGACCTGTATCAGGTGGGTCGATGAACCCTGTGAGGACTTTAGGACCTGCAATGGCAAGTGAATATTATAAAGGACTTTGGGTGTACTTTGTCGGGCCGGTTACAGGAACCCTTTTGGGGGCATGGTCATATAAGTTCATACGTGTCAGTGACAAACCTGTGCATCTAATTTCTCCTCATTCATTTTCACTCAAACTTCGAAGAATGTCGAGATCGGACACTGCTGAAAGTGAAAATTGAGCATTAGCTCAACATTCTCTTCTACCATCTTCAAAATGTTTGGTGTGTCGGAAAACATATGTGAAATAGTTTGAGTGTTTCATGAGCTTCGTGATGTACCATGCTCTAAGCTTACTTTCTTAGAGTTTAGCAATGTATAAGAAGTAGCCTTTGTAATTTGCTTAGCATCTTCATTGGTATATTTTCTAAATTTTGTAACTTTCCTTTTCCAAATAATAGCCAAATTCTAGAAATGGAATAGTTCACTTT

mRNA sequence

ATTTTCTAATGGCGAGAGGTTGAATTAAAAATTATATATATTTTTATAATAGATTCATAATACAAAGAGACACTGATGTGTAACTACTTGAACCGTACACCCATCCTCCATTTCACTATAAAATGGGTCGCTTGGTTCCACCTATTGATGTGCAGAGAGAGGCTTTTTTTGTTTGAGTATATATTAATATTTTAGGTTGAAAAATGGGTTCGGTTCATGATCCGGAGGCCGGCGACGGTTACCGCAGCGGCGGCGCTGCCACCGCCGACGTGGAGGAGTTCGTGTCAGTAGAAAACCCAGACTCCAAACTCTCCAAGTTTGGATCTATCTTCCAAAACCTTTACCCTCCTGGGTTTTCCCGAAAGCTGGTGGCGGAAGTGATAGCGACATATTTGCTGGTGTTTGTGACGTGCGGGGCGGGGGCATTGAGCGCGAGCGACGAGCGGAGAGTGTCGCAGCTCGGAGCCTCAGTCGCGGGCGGGCTGATCGTCACCGTGATGATCTACGCTGTCGGCCACATATCGGGCGCCCACATGAACCCGGCTGTCACCTTAGCTTTCGCTGCAACTCGACACTTTCCATGGAAACAGGTTCCAGCGTATGCAGCAGCGCAGCTGAGTGGAGCAACATCTGCGGCCTTTACGCTGCGAATATTGCTGCATCCAATCAAACACGTGGGCACAACGTCGCCATCCGGATCGGATTTTCAGGCATTGGTCATGGAGATAGTTGTTACATTCTCCATGATGTTCGTTACTTCAGCTGTCGCAACCGACACCAAAGCTGTGGGAGAGCTAGCAGGTATGGCAGTTGGCTCAGCTGTGTGTATTACATCCATCTTAGCTGGACCTGTATCAGGTGGGTCGATGAACCCTGTGAGGACTTTAGGACCTGCAATGGCAAGTGAATATTATAAAGGACTTTGGGTGTACTTTGTCGGGCCGGTTACAGGAACCCTTTTGGGGGCATGGTCATATAAGTTCATACGTGTCAGTGACAAACCTGTGCATCTAATTTCTCCTCATTCATTTTCACTCAAACTTCGAAGAATGTCGAGATCGGACACTGCTGAAAGTGAAAATTGAGCATTAGCTCAACATTCTCTTCTACCATCTTCAAAATGTTTGGTGTGTCGGAAAACATATGTGAAATAGTTTGAGTGTTTCATGAGCTTCGTGATGTACCATGCTCTAAGCTTACTTTCTTAGAGTTTAGCAATGTATAAGAAGTAGCCTTTGTAATTTGCTTAGCATCTTCATTGGTATATTTTCTAAATTTTGTAACTTTCCTTTTCCAAATAATAGCCAAATTCTAGAAATGGAATAGTTCACTTT

Coding sequence (CDS)

ATGGGTTCGGTTCATGATCCGGAGGCCGGCGACGGTTACCGCAGCGGCGGCGCTGCCACCGCCGACGTGGAGGAGTTCGTGTCAGTAGAAAACCCAGACTCCAAACTCTCCAAGTTTGGATCTATCTTCCAAAACCTTTACCCTCCTGGGTTTTCCCGAAAGCTGGTGGCGGAAGTGATAGCGACATATTTGCTGGTGTTTGTGACGTGCGGGGCGGGGGCATTGAGCGCGAGCGACGAGCGGAGAGTGTCGCAGCTCGGAGCCTCAGTCGCGGGCGGGCTGATCGTCACCGTGATGATCTACGCTGTCGGCCACATATCGGGCGCCCACATGAACCCGGCTGTCACCTTAGCTTTCGCTGCAACTCGACACTTTCCATGGAAACAGGTTCCAGCGTATGCAGCAGCGCAGCTGAGTGGAGCAACATCTGCGGCCTTTACGCTGCGAATATTGCTGCATCCAATCAAACACGTGGGCACAACGTCGCCATCCGGATCGGATTTTCAGGCATTGGTCATGGAGATAGTTGTTACATTCTCCATGATGTTCGTTACTTCAGCTGTCGCAACCGACACCAAAGCTGTGGGAGAGCTAGCAGGTATGGCAGTTGGCTCAGCTGTGTGTATTACATCCATCTTAGCTGGACCTGTATCAGGTGGGTCGATGAACCCTGTGAGGACTTTAGGACCTGCAATGGCAAGTGAATATTATAAAGGACTTTGGGTGTACTTTGTCGGGCCGGTTACAGGAACCCTTTTGGGGGCATGGTCATATAAGTTCATACGTGTCAGTGACAAACCTGTGCATCTAATTTCTCCTCATTCATTTTCACTCAAACTTCGAAGAATGTCGAGATCGGACACTGCTGAAAGTGAAAATTGA

Protein sequence

MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN

Homology

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BLAST of MC08g1398.1 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 315.8 bits (808), Expect = 5.0e-85
Identity = 162/273 (59.34%), Postives = 206/273 (75.46%), Query Frame = 0


Query: 22  DVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDER 81
           ++ +  + +N     + +     +++PP   +K+V+EV++T+LLVFVTCGA  +  SD+ 
Sbjct: 16  EIHDIGTAQNSSMPPTYYDRSLADIFPPHLLKKVVSEVVSTFLLVFVTCGAAGIYGSDKD 75

Query: 82  RVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGA 141
           R+SQLG SVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA  RHFPW QVP Y AAQ +G+
Sbjct: 76  RISQLGQSVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGS 135

Query: 142 TSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGM 201
             A+F L+ +LHPI  +GTT+P+G  + +LV+EI+VTF+MMFVT AVATDT+AVGELAG+
Sbjct: 136 ICASFVLKAVLHPIAVLGTTTPTGPHWHSLVIEIIVTFNMMFVTLAVATDTRAVGELAGL 195

Query: 202 AVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFI 261
           AVGSAVCITSI AG VSGGSMNP RTLGPA+AS  Y GLW+YF+GPV GTL GAW+Y +I
Sbjct: 196 AVGSAVCITSIFAGAVSGGSMNPARTLGPALASNLYTGLWIYFLGPVLGTLSGAWTYTYI 255

Query: 262 RVSDKPVHL-ISPHSFSLKLRRMSRSDTAESEN 294
           R  + P H  +S    S KLRR+     A  ++
Sbjct: 256 RFEEAPSHKDMSQKLSSFKLRRLQSQSVAVDDD 288

[Close]

BLAST of MC08g1398.1 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 8.5e-85
Identity = 160/246 (65.04%), Postives = 193/246 (78.46%), Query Frame = 0


Query: 45  NLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVG 104
           +++PP   +K+++EV+AT+LLVFVTCGA ++   D RR+SQLG SVAGGLIVTVMIYA G
Sbjct: 44  DIFPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNRRISQLGQSVAGGLIVTVMIYATG 103

Query: 105 HISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPS 164
           HISGAHMNPAVTL+FA  RHFPW QVP Y AAQ +GA  AAF L+ +LHPI  +GTT+PS
Sbjct: 104 HISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLHPIAVIGTTTPS 163

Query: 165 GSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNP 224
           G  + AL++EIVVTF+MMFVT AVATD++AVGELAG+AVGSAVCITSI AGPVSGGSMNP
Sbjct: 164 GPHWHALLIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNP 223

Query: 225 VRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMS 284
            RTL PA+AS  + GLW+YF+GPV GTL GAW Y +IR  + P    +    S KLRRM 
Sbjct: 224 ARTLAPAVASNVFTGLWIYFLGPVIGTLSGAWVYTYIRFEEAPAAKDTQRLSSFKLRRMQ 283

Query: 285 RSDTAE 291
               A+
Sbjct: 284 SQLAAD 289

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BLAST of MC08g1398.1 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.4e-84
Identity = 164/279 (58.78%), Postives = 205/279 (73.48%), Query Frame = 0


Query: 22  DVEEFVSVENPDSKLSKFG-SIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDE 81
           ++ +  +V+N       +G     + +PP   +K+V+EV+AT+LLVF+TCGA  +S SD 
Sbjct: 17  EIHDLSTVQNGTMPTMYYGEKAIADFFPPHLLKKVVSEVVATFLLVFMTCGAAGISGSDL 76

Query: 82  RRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSG 141
            R+SQLG S+AGGLIVTVMIYAVGHISGAHMNPAVTLAFA  RHFPW QVP Y AAQ +G
Sbjct: 77  SRISQLGQSIAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTG 136

Query: 142 ATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAG 201
           A  A+F L+ ++HP+  +GTT+P G  + +LV+E++VTF+MMFVT AVATDT+AVGELAG
Sbjct: 137 AICASFVLKAVIHPVDVIGTTTPVGPHWHSLVVEVIVTFNMMFVTLAVATDTRAVGELAG 196

Query: 202 MAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKF 261
           +AVGSAVCITSI AG +SGGSMNP RTLGPA+AS  + GLW+YF+GPV GTL GAW+Y F
Sbjct: 197 LAVGSAVCITSIFAGAISGGSMNPARTLGPALASNKFDGLWIYFLGPVMGTLSGAWTYTF 256

Query: 262 IRVSDKPVHLISPHSFSLKLRRM------SRSDTAESEN 294
           IR  D P    S    S KLRR+      +  D  E EN
Sbjct: 257 IRFEDTPKEGSSQKLSSFKLRRLRSQQSIAADDVDEMEN 295

[Close]

BLAST of MC08g1398.1 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.7e-83
Identity = 161/253 (63.64%), Postives = 195/253 (77.08%), Query Frame = 0


Query: 43  FQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYA 102
           F +++PP   +K+++EV+AT+LLVFVTCGA ++   D +R+SQLG SV GGLIVTVMIYA
Sbjct: 42  FADIFPPNLLKKVISEVVATFLLVFVTCGAASIYGEDMKRISQLGQSVVGGLIVTVMIYA 101

Query: 103 VGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTS 162
            GHISGAHMNPAVTL+FA  RHFPW QVP Y AAQ +GA  AAF LR +L+PI+ +GTT+
Sbjct: 102 TGHISGAHMNPAVTLSFAFFRHFPWIQVPFYWAAQFTGAMCAAFVLRAVLYPIEVLGTTT 161

Query: 163 PSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSM 222
           P+G  + ALV+EIVVTF+MMFVT AVATD++AVGELAG+AVGSAVCITSI AGPVSGGSM
Sbjct: 162 PTGPHWHALVIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSM 221

Query: 223 NPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIRVSDKPVHL--ISPHSF-SLK 282
           NP RTL PA+AS  Y GLW+YF+GPV GTL GAW Y +IR  + P      +P    S K
Sbjct: 222 NPARTLAPAVASNVYTGLWIYFLGPVVGTLSGAWVYTYIRFEEAPAAAGGAAPQKLSSFK 281

Query: 283 LRRMSRSDTAESE 293
           LRR+     A  E
Sbjct: 282 LRRLQSQSMAADE 294

[Close]

BLAST of MC08g1398.1 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.1e-82
Identity = 173/292 (59.25%), Postives = 206/292 (70.55%), Query Frame = 0


Query: 4   VHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATY 63
           +HD     G   G AA A    F     PDSK         +++PP   +K+++EV+AT+
Sbjct: 19  IHDLSTVQG---GSAAAAAAALFY----PDSK------SIADIFPPHLGKKVISEVVATF 78

Query: 64  LLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATR 123
           LLVFVTCGA ++   D  R+SQLG SVAGGLIVTVMIYA GHISGAHMNPAVTL+FA  R
Sbjct: 79  LLVFVTCGAASIYGEDNARISQLGQSVAGGLIVTVMIYATGHISGAHMNPAVTLSFACFR 138

Query: 124 HFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMF 183
           HFPW QVP Y AAQ +GA  AAF L+ +L PI  +GTT+PSG  + AL +EIVVTF+MMF
Sbjct: 139 HFPWIQVPFYWAAQFTGAMCAAFVLKAVLQPIAVIGTTTPSGPHWHALAIEIVVTFNMMF 198

Query: 184 VTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVY 243
           VT AVATD++AVGELAG+AVGSAVCITSI AGPVSGGSMNP RTL PA+AS  + GLW+Y
Sbjct: 199 VTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNPARTLAPAVASNVFTGLWIY 258

Query: 244 FVGPVTGTLLGAWSYKFIRVSDKPVHL--ISPHSFSLKLRRM-SRSDTAESE 293
           F+GPV GTL GAW Y +IR  + P      +    S KLRRM S+S  A  E
Sbjct: 259 FLGPVVGTLSGAWVYTYIRFEEAPAAAKPDTQRLSSFKLRRMQSQSALAADE 297

[Close]

BLAST of MC08g1398.1 vs. NCBI nr
Match: XP_022149783.1 (aquaporin NIP2-1-like [Momordica charantia])

HSP 1 Score: 563 bits (1451), Expect = 5.42e-202
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI 60
           MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI
Sbjct: 1   MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI 60

Query: 61  ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA 120
           ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA
Sbjct: 61  ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA 120

Query: 121 ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS 180
           ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS
Sbjct: 121 ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS 180

Query: 181 MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL 240
           MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL
Sbjct: 181 MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL 240

Query: 241 WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN 293
           WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN
Sbjct: 241 WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN 293

[Close]

BLAST of MC08g1398.1 vs. NCBI nr
Match: XP_022983845.1 (aquaporin NIP2-1-like [Cucurbita maxima])

HSP 1 Score: 478 bits (1230), Expect = 2.14e-168
Identity = 247/270 (91.48%), Postives = 257/270 (95.19%), Query Frame = 0


Query: 23  VEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDERR 82
           V++ VS+ENPDSK S F S+FQN YPPGFSRKLVAEVIATYLLVFVTCGA ALSASDERR
Sbjct: 18  VQDLVSLENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDERR 77

Query: 83  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGAT 142
           VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVP Y AAQLSGAT
Sbjct: 78  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGAT 137

Query: 143 SAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGMA 202
            AAFTLRILLHPIK +GTT+PSG+DFQALVMEIVVTFSMMFVTSAVATDTKA+GELAGMA
Sbjct: 138 CAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMA 197

Query: 203 VGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIR 262
           VGSAVCITSILAGPVSGGSMNPVRTLGPA+ASEYYKGLWVYFVGPVTGTLLGAWSYKFIR
Sbjct: 198 VGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIR 257

Query: 263 VSDKPVHLISPHSFSLKLRRMSRSDTAESE 292
            SDKPVHLISPHSFS KLRRMSRSD +ESE
Sbjct: 258 ASDKPVHLISPHSFSFKLRRMSRSDVSESE 287

[Close]

BLAST of MC08g1398.1 vs. NCBI nr
Match: NP_001267699.1 (aquaporin NIP2-1-like [Cucumis sativus] >ACU29603.1 Si transport-like protein 1 [Cucumis sativus] >AOW42547.1 silicon transporter Lsi1 [Cucumis sativus] >APT69293.1 LSi1 [Cucumis sativus var. sativus] >KGN59570.1 hypothetical protein Csa_001739 [Cucumis sativus])

HSP 1 Score: 477 bits (1227), Expect = 6.13e-168
Identity = 248/293 (84.64%), Postives = 264/293 (90.10%), Query Frame = 0


Query: 1   MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI 60
           M S+ +P+      S   A  DV EFVSVENPDSK SKFGS F+N YPPGFSRKLVAEVI
Sbjct: 1   MSSIQNPQL-----SNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVI 60

Query: 61  ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA 120
           ATYLLVFVTCGA AL+ASD RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT+AFA
Sbjct: 61  ATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFA 120

Query: 121 ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS 180
           ATRHFPWKQVP Y AAQLSGAT AAFTLR+LLHPIKH+GTT+PSGSD QALVMEIVVTFS
Sbjct: 121 ATRHFPWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFS 180

Query: 181 MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL 240
           MMFVT AVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPA+AS+YYKGL
Sbjct: 181 MMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGL 240

Query: 241 WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN 293
           WVYFVGPV GT LGAWSYKFIR SDKPVHLISPHSFSLK+RRMSRSD +ES +
Sbjct: 241 WVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288

[Close]

BLAST of MC08g1398.1 vs. NCBI nr
Match: XP_023529347.1 (aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo] >KAG7022218.1 Aquaporin NIP2-1 [Cucurbita argyrosperma subsp. argyrosperma] >CAD67694.1 Nod26-like protein [Cucurbita pepo])

HSP 1 Score: 476 bits (1226), Expect = 8.70e-168
Identity = 246/274 (89.78%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 19  ATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSAS 78
           +  DVEEFVSVENPDSK S+FGS+F+N YPPGFSRKLVAEVIATYLLVFVTCGA AL+ S
Sbjct: 14  SVVDVEEFVSVENPDSKRSQFGSLFKNHYPPGFSRKLVAEVIATYLLVFVTCGAAALNGS 73

Query: 79  DERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQL 138
           D +RVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVT AFAATRHFPWKQVP YAAAQL
Sbjct: 74  DAQRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTTAFAATRHFPWKQVPLYAAAQL 133

Query: 139 SGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGEL 198
           SGAT AAFTLR+LLHPIKH+GTT+PSGSD QALVMEIVVTFSMMFVT AVATDTKAVGEL
Sbjct: 134 SGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVTCAVATDTKAVGEL 193

Query: 199 AGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSY 258
           AG+AVGSAVCITSILAGPVSGGSMNPVRTLGPAMAS+ YKGLWVYFVGPVTGTLLGAWSY
Sbjct: 194 AGLAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASDNYKGLWVYFVGPVTGTLLGAWSY 253

Query: 259 KFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESE 292
           KFIR SDKPVHLISPHSFSLKLRRMSRSD  E E
Sbjct: 254 KFIRASDKPVHLISPHSFSLKLRRMSRSDVGEGE 287

[Close]

BLAST of MC08g1398.1 vs. NCBI nr
Match: XP_022934054.1 (aquaporin NIP2-1-like [Cucurbita moschata])

HSP 1 Score: 476 bits (1225), Expect = 1.24e-167
Identity = 246/268 (91.79%), Postives = 255/268 (95.15%), Query Frame = 0


Query: 23  VEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDERR 82
           V++ VSVENPDSK S F S+FQN YPPGFSRKLVAEVIATYLLVFVTCGA ALSASDERR
Sbjct: 18  VQDLVSVENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDERR 77

Query: 83  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGAT 142
           VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVP Y AAQLSGAT
Sbjct: 78  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGAT 137

Query: 143 SAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGMA 202
            AAFTLRILLHPIK +GTT+PSG+DFQALVMEIVVTFSMMFVTSAVATDTKA+GELAGMA
Sbjct: 138 CAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMA 197

Query: 203 VGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIR 262
           VGSAVCITSILAGPVSGGSMNPVRTLGPA+ASEYYKGLWVYFVGPVTGTLLGAWSYKFIR
Sbjct: 198 VGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIR 257

Query: 263 VSDKPVHLISPHSFSLKLRRMSRSDTAE 290
            SDKPVHLISPHSFS KLRRMSRSD +E
Sbjct: 258 ASDKPVHLISPHSFSFKLRRMSRSDVSE 285

[Close]

BLAST of MC08g1398.1 vs. ExPASy TrEMBL
Match: A0A6J1D7P6 (aquaporin NIP2-1-like OS=Momordica charantia OX=3673 GN=LOC111018132 PE=3 SV=1)

HSP 1 Score: 563 bits (1451), Expect = 2.63e-202
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI 60
           MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI
Sbjct: 1   MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI 60

Query: 61  ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA 120
           ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA
Sbjct: 61  ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA 120

Query: 121 ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS 180
           ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS
Sbjct: 121 ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS 180

Query: 181 MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL 240
           MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL
Sbjct: 181 MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL 240

Query: 241 WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN 293
           WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN
Sbjct: 241 WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN 293

[Close]

BLAST of MC08g1398.1 vs. ExPASy TrEMBL
Match: A0A6J1J724 (aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482339 PE=3 SV=1)

HSP 1 Score: 478 bits (1230), Expect = 1.04e-168
Identity = 247/270 (91.48%), Postives = 257/270 (95.19%), Query Frame = 0


Query: 23  VEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDERR 82
           V++ VS+ENPDSK S F S+FQN YPPGFSRKLVAEVIATYLLVFVTCGA ALSASDERR
Sbjct: 18  VQDLVSLENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDERR 77

Query: 83  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGAT 142
           VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVP Y AAQLSGAT
Sbjct: 78  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGAT 137

Query: 143 SAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGMA 202
            AAFTLRILLHPIK +GTT+PSG+DFQALVMEIVVTFSMMFVTSAVATDTKA+GELAGMA
Sbjct: 138 CAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMA 197

Query: 203 VGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIR 262
           VGSAVCITSILAGPVSGGSMNPVRTLGPA+ASEYYKGLWVYFVGPVTGTLLGAWSYKFIR
Sbjct: 198 VGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIR 257

Query: 263 VSDKPVHLISPHSFSLKLRRMSRSDTAESE 292
            SDKPVHLISPHSFS KLRRMSRSD +ESE
Sbjct: 258 ASDKPVHLISPHSFSFKLRRMSRSDVSESE 287

[Close]

BLAST of MC08g1398.1 vs. ExPASy TrEMBL
Match: G8XUV3 (Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1)

HSP 1 Score: 477 bits (1227), Expect = 2.97e-168
Identity = 248/293 (84.64%), Postives = 264/293 (90.10%), Query Frame = 0


Query: 1   MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI 60
           M S+ +P+      S   A  DV EFVSVENPDSK SKFGS F+N YPPGFSRKLVAEVI
Sbjct: 1   MSSIQNPQL-----SNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVI 60

Query: 61  ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA 120
           ATYLLVFVTCGA AL+ASD RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT+AFA
Sbjct: 61  ATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFA 120

Query: 121 ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS 180
           ATRHFPWKQVP Y AAQLSGAT AAFTLR+LLHPIKH+GTT+PSGSD QALVMEIVVTFS
Sbjct: 121 ATRHFPWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFS 180

Query: 181 MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL 240
           MMFVT AVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPA+AS+YYKGL
Sbjct: 181 MMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGL 240

Query: 241 WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN 293
           WVYFVGPV GT LGAWSYKFIR SDKPVHLISPHSFSLK+RRMSRSD +ES +
Sbjct: 241 WVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288

[Close]

BLAST of MC08g1398.1 vs. ExPASy TrEMBL
Match: A0A1L7B7J0 (LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1)

HSP 1 Score: 477 bits (1227), Expect = 2.97e-168
Identity = 248/293 (84.64%), Postives = 264/293 (90.10%), Query Frame = 0


Query: 1   MGSVHDPEAGDGYRSGGAATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVI 60
           M S+ +P+      S   A  DV EFVSVENPDSK SKFGS F+N YPPGFSRKLVAEVI
Sbjct: 1   MSSIQNPQL-----SNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVI 60

Query: 61  ATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA 120
           ATYLLVFVTCGA AL+ASD RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT+AFA
Sbjct: 61  ATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFA 120

Query: 121 ATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFS 180
           ATRHFPWKQVP Y AAQLSGAT AAFTLR+LLHPIKH+GTT+PSGSD QALVMEIVVTFS
Sbjct: 121 ATRHFPWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFS 180

Query: 181 MMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGL 240
           MMFVT AVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPA+AS+YYKGL
Sbjct: 181 MMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGL 240

Query: 241 WVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESEN 293
           WVYFVGPV GT LGAWSYKFIR SDKPVHLISPHSFSLK+RRMSRSD +ES +
Sbjct: 241 WVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288

[Close]

BLAST of MC08g1398.1 vs. ExPASy TrEMBL
Match: Q7X9T0 (Nod26-like protein OS=Cucurbita pepo OX=3663 GN=NIP1 PE=2 SV=1)

HSP 1 Score: 476 bits (1226), Expect = 4.21e-168
Identity = 246/274 (89.78%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 19  ATADVEEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSAS 78
           +  DVEEFVSVENPDSK S+FGS+F+N YPPGFSRKLVAEVIATYLLVFVTCGA AL+ S
Sbjct: 14  SVVDVEEFVSVENPDSKRSQFGSLFKNHYPPGFSRKLVAEVIATYLLVFVTCGAAALNGS 73

Query: 79  DERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQL 138
           D +RVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVT AFAATRHFPWKQVP YAAAQL
Sbjct: 74  DAQRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTTAFAATRHFPWKQVPLYAAAQL 133

Query: 139 SGATSAAFTLRILLHPIKHVGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGEL 198
           SGAT AAFTLR+LLHPIKH+GTT+PSGSD QALVMEIVVTFSMMFVT AVATDTKAVGEL
Sbjct: 134 SGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVTCAVATDTKAVGEL 193

Query: 199 AGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSY 258
           AG+AVGSAVCITSILAGPVSGGSMNPVRTLGPAMAS+ YKGLWVYFVGPVTGTLLGAWSY
Sbjct: 194 AGLAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASDNYKGLWVYFVGPVTGTLLGAWSY 253

Query: 259 KFIRVSDKPVHLISPHSFSLKLRRMSRSDTAESE 292
           KFIR SDKPVHLISPHSFSLKLRRMSRSD  E E
Sbjct: 254 KFIRASDKPVHLISPHSFSLKLRRMSRSDVGEGE 287

[Close]

BLAST of MC08g1398.1 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 224.6 bits (571), Expect = 1.1e-58
Identity = 120/244 (49.18%), Postives = 163/244 (66.80%), Query Frame = 0


Query: 51  FSRKLVAEVIATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAH 110
           F +KL+AEV+ TY L+F  C A A++   ++ V+  G ++  GL V V++Y++GHISGAH
Sbjct: 50  FLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISGAH 109

Query: 111 MNPAVTLAFAATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGT--------TS 170
            NPAVT+AFA+   FP KQVPAY  +Q+ G+T AA TLR+L    + V +        T 
Sbjct: 110 FNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGTL 169

Query: 171 PSGSDFQALVMEIVVTFSMMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSM 230
           PSGS+ Q+ V+E ++TF +MFV S VATD +A+GELAG+AVGS V +  I+AGPVSG SM
Sbjct: 170 PSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGASM 229

Query: 231 NPVRTLGPAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLKLRR 287
           NP R+LGPAM    Y+GLW+Y V P+ G + GAW Y  +R +DKP+  I+     LK  R
Sbjct: 230 NPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTDKPLREITKSGSFLKTVR 289

[Close]

BLAST of MC08g1398.1 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 217.2 bits (552), Expect = 1.7e-56
Identity = 123/269 (45.72%), Postives = 171/269 (63.57%), Query Frame = 0


Query: 24  EEFVSVENPDSKLSKFGSIFQNLYPPGFSRKLVAEVIATYLLVFVTCGAGALSASDERRV 83
           +E   + NP   L K  S+     P  F +KL+AE + TY LVF  C +  ++  ++  V
Sbjct: 29  QEMEDIHNP-RPLKKQDSLLSVSVP--FLQKLIAEFLGTYFLVFTGCASVVVNMQNDNVV 88

Query: 84  SQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPAYAAAQLSGATS 143
           +  G ++  GL + V+IY++GHISGAH+NPAVT+AFA+   FP KQVPAY  +Q+ G+T 
Sbjct: 89  TLPGIAIVWGLTIMVLIYSLGHISGAHINPAVTIAFASCGRFPLKQVPAYVISQVIGSTL 148

Query: 144 AAFTLRILL---HPI---KH---VGTTSPSGSDFQALVMEIVVTFSMMFVTSAVATDTKA 203
           AA TLR+L    H +   KH   +G +SP GSD QA  ME +VTF +MF+ S VATD +A
Sbjct: 149 AAATLRLLFGLDHDVCSGKHDVFIG-SSPVGSDLQAFTMEFIVTFYLMFIISGVATDNRA 208

Query: 204 VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASEYYKGLWVYFVGPVTGTLLG 263
           +GELAG+A+GS V +  ++A PVS  SMNP R+LGPA+    YKG+W+Y V P  G + G
Sbjct: 209 IGELAGLAIGSTVLLNVLIAAPVSSASMNPGRSLGPALVYGCYKGIWIYLVAPTLGAIAG 268

Query: 264 AWSYKFIRVSDKPVHLISPHSFSLKLRRM 284
           AW Y  +R +DKP+  I+     LK  R+
Sbjct: 269 AWVYNTVRYTDKPLREITKSGSFLKTVRI 293

[Close]

BLAST of MC08g1398.1 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 211.8 bits (538), Expect = 7.2e-55
Identity = 118/245 (48.16%), Postives = 159/245 (64.90%), Query Frame = 0


Query: 52  SRKLVAEVIATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHM 111
           ++KL+AE+I TY ++F  CG   ++      ++  G  V  GLIV VMIY+ GHISGAH 
Sbjct: 42  TQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHF 101

Query: 112 NPAVTLAFAATRHFPWKQVPAYAAAQLSGATSAAFTLRILLH--PIKHVGTTSPSGSDFQ 171
           NPAVT+ FA  R FPW QVP Y  AQL+G+  A+ TLR++ +  P    GTT P+ S  Q
Sbjct: 102 NPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTT-PTDSSGQ 161

Query: 172 ALVMEIVVTFSMMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLG 231
           ALV EI+++F +MFV S VATD++A GELAG+AVG  + +   +AGP+SG SMNP R+LG
Sbjct: 162 ALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLG 221

Query: 232 PAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIRVSDKPV-HLISPHSFSLKLRRMSRSDT 291
           PA+    YKG+WVY VGP  G   G + Y F+R +DKP+  L    SF   LR +++ D 
Sbjct: 222 PAIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDKPLRELTKSASF---LRSVAQKDN 281

Query: 292 AESEN 294
           A   +
Sbjct: 282 ASKSD 282

[Close]

BLAST of MC08g1398.1 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-53
Identity = 112/230 (48.70%), Postives = 152/230 (66.09%), Query Frame = 0


Query: 52  SRKLVAEVIATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHM 111
           ++KL+AE+I TY +VF  CG   ++      ++  G  V  GLIV VMIY+ GHISGAH 
Sbjct: 42  TQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHF 101

Query: 112 NPAVTLAFAATRHFPWKQVPAYAAAQLSGATSAAFTLRIL--LHPIKHVGTTSPSGSDFQ 171
           NPAVT+ FA  R FPW QVP Y  AQ +G+  A+ TLR++  + P    GTT P+ S  +
Sbjct: 102 NPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTT-PADSPAR 161

Query: 172 ALVMEIVVTFSMMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLG 231
           ALV EI+++F +MFV S VATD +AVGELAG+AVG  + +   +AGP+SG SMNP R+LG
Sbjct: 162 ALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLG 221

Query: 232 PAMASEYYKGLWVYFVGPVTGTLLGAWSYKFIRVSDKPVHLISPHSFSLK 280
           PA+    YK +WVY VGPV G + G + Y  IR +DKP+  ++  +  L+
Sbjct: 222 PALVMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDKPLRELTKSASFLR 270

[Close]

BLAST of MC08g1398.1 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 205.3 bits (521), Expect = 6.7e-53
Identity = 104/214 (48.60%), Postives = 147/214 (68.69%), Query Frame = 0


Query: 52  SRKLVAEVIATYLLVFVTCGAGALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHM 111
           +RKL AE + T++L+F       ++   +   + +G +   GL V ++I + GHISGAH+
Sbjct: 77  TRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISGAHL 136

Query: 112 NPAVTLAFAATRHFPWKQVPAYAAAQLSGATSAAFTLRILLHPIKHVGTTSPSGSDFQAL 171
           NP++T+AFAA RHFPW  VPAY AAQ+S +  A+F L+ + HP    G T PS S  QA 
Sbjct: 137 NPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLGQAF 196

Query: 172 VMEIVVTFSMMFVTSAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPA 231
            +E ++TF ++FV +AVATDT+AVGELAG+AVG+ V +  ++AGP +GGSMNPVRTLGPA
Sbjct: 197 ALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPA 256

Query: 232 MASEYYKGLWVYFVGPVTGTLLGAWSYKFIRVSD 266
           +AS  Y+ LWVY V P  G + GA  Y  ++++D
Sbjct: 257 VASGNYRSLWVYLVAPTLGAISGAAVYTGVKLND 290

The following BLAST results are available for this feature:

BLAST of MC08g1398.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q19KC1	5.0e-85	59.34	Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2	[more]
Q9ATN2	8.5e-85	65.04	Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1	[more]
Q6Z2T3	1.4e-84	58.78	Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1	[more]
Q67WJ8	2.7e-83	63.64	Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1	[more]
Q9AT74	5.1e-82	59.25	Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1	[more]

BLAST of MC08g1398.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149783.1	5.42e-202	100.00	aquaporin NIP2-1-like [Momordica charantia]	[more]
XP_022983845.1	2.14e-168	91.48	aquaporin NIP2-1-like [Cucurbita maxima]	[more]
NP_001267699.1	6.13e-168	84.64	aquaporin NIP2-1-like [Cucumis sativus] >ACU29603.1 Si transport-like protein 1 ...	[more]
XP_023529347.1	8.70e-168	89.78	aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo] >KAG7022218.1 Aquaporin NIP2-...	[more]
XP_022934054.1	1.24e-167	91.79	aquaporin NIP2-1-like [Cucurbita moschata]	[more]

BLAST of MC08g1398.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7P6	2.63e-202	100.00	aquaporin NIP2-1-like OS=Momordica charantia OX=3673 GN=LOC111018132 PE=3 SV=1	[more]
A0A6J1J724	1.04e-168	91.48	aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482339 PE=3 SV=1	[more]
G8XUV3	2.97e-168	84.64	Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1	[more]
A0A1L7B7J0	2.97e-168	84.64	LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1	[more]
Q7X9T0	4.21e-168	89.78	Nod26-like protein OS=Cucurbita pepo OX=3663 GN=NIP1 PE=2 SV=1	[more]

BLAST of MC08g1398.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18910.1	1.1e-58	49.18	NOD26-like intrinsic protein 1;2	[more]
AT4G19030.1	1.7e-56	45.72	NOD26-like major intrinsic protein 1	[more]
AT5G37820.1	7.2e-55	48.16	NOD26-like intrinsic protein 4;2	[more]
AT5G37810.1	1.4e-53	48.70	NOD26-like intrinsic protein 4;1	[more]
AT4G10380.1	6.7e-53	48.60	NOD26-like intrinsic protein 5;1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 53..72 score: 38.08 coord: 241..261 score: 36.26 coord: 92..116 score: 50.67 coord: 173..191 score: 27.2 coord: 204..226 score: 34.94 coord: 129..148 score: 33.8
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 51..258 e-value: 2.0E-52 score: 178.2
IPR000425	Major intrinsic protein	CDD	cd00333	MIP	coord: 53..261 e-value: 8.46999E-54 score: 172.823
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 22..268 e-value: 7.4E-67 score: 226.9
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 46..271
None	No IPR available	PANTHER	PTHR45724:SF30	MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATED	coord: 22..277
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 22..277
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 110..118

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC08g1398	MC08g1398	gene

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g1398.1-utr3	MC08g1398.1-utr3-MC08:14723573..14723821	utr3

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g1398.1-cds	MC08g1398.1-cds-MC08:14723822..14724059	CDS
MC08g1398.1-cds	MC08g1398.1-cds-MC08:14724248..14724309	CDS
MC08g1398.1-cds	MC08g1398.1-cds-MC08:14724978..14725172	CDS
MC08g1398.1-cds	MC08g1398.1-cds-MC08:14728098..14728322	CDS
MC08g1398.1-cds	MC08g1398.1-cds-MC08:14728518..14728679	CDS

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g1398.1-utr5	MC08g1398.1-utr5-MC08:14728680..14728882	utr5

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC08g1398.1	MC08g1398.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0015267	channel activity