MC05g_new0026.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0026.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Glutathione transferase
Location	MC05: 2124250 .. 2128265 (-)
Sequence length	693
RNA-Seq Expression	MC05g_new0026.1
Synteny	MC05g_new0026.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCTCTTCTCTCTCATCTCAATATATAAATAGAAATTCAGCGCACCAGTCTTTTAAACATTAACTGCCTTGGTTCCACTTCAAGAAGCGTCGCGGGCAGCTGCTTCTCGCGTTGACGGCCATGTACTCCGAAGCTCAAGCTCAGGTACCTTCACTCTCTCGAGCGTGTGCGGTTTTCTTGAAAATCGTTTAATCTGAAGATAATTGCGGAGAGACTGATTCATTTTCTCACTCGAAATCATTAGGAAATGTAGTAAGATGTTGATCGTGCATCTGTTTTTGTTGTAGCAGAAAAGCTCTGACGATTCGTCGACGTCTTCGAAGCTGGTTCTTTATTCTTTCTGGCAGAGCTCCTGCTCGTGGCGTGTTCGCTTTGCGTTGAATCTCAAAGGTATTTCTTCTTCTTTGAGTTTATTGGGAAAATTTTCTTCGATTTTGAAGTACGGATGAATTGGAGTCTGATTTGTCGTAGAACGCTTAGTAATCTTGTTTTAGTTGAGTCTAATTAGCTGACGCGAGGATTGTGATAGTAAATCAAATGAATGAAGAATTCGAGTTTGAATTTCTGTTACTCTCGGTTGTCCTATGAAGATTGTGATAGTAAATAAAACGAATGAAGAGTTGGAGTTTGAAGTACCGAAGATCAGTTCGAGTTTCACTTGTCGCAAAACGCACAATAATCTTGTGTAAATTGATTCTAAGCTGACACGAAGATTGTGATGATAGATCAAGCGAATGAAAGTTGTGGAACTTGATCCTTTTTTTTAACAGATGTTTTACGATTGTTGCTTAGGGCTTTCTTACGAGTACAGAGCAGTAAACCTTGGGAGAGGAGAACACTTAAGTCCAGGTAAATCTCTAGATTGATGTGTGTTTGTGCTAATTTATAGAATCAAAAAAAGGCTGAACAACTTTCTGCTTCCGAGATGCAGATTTTGAGCGCCTAAATCCACTTAAATATGTTCCTATATTAGTAGATGGTCCAGTGGTAGTTTCGGACTCTTACGCGATATTTCTGGTAAGGTTTAAAGAAAAGGGAGATGAGTCAGTTCAAGTCTGTGACGAAGAACAACGAACTTCTGAATTGGCCGTAGTGGCCTGGCAAATGCCTATTCAGAAGATTTTTTATTCTCACGGACTTGAATCTACGAGTTATTCTTTTCTCATGCCCAAGTTTTGATCCCCTGTTGCTCTTATCATAAATTGTCCTTGTAACATCATTGCAATTTTATTGCTATTCTTTAGTATTTGGAAGAGAAGTATCCACAGAAGGCGCTGCTGCCTGCTGATCTTCATTCAAAATGTCTGAATCTGCAAGTAAGATCAGTAGTGGTTAGCTACAAATCGAGAGGTTTAAAAGAGCTCTCTCGATCTATAAAAATGTCTTTCTAGCATTAAACTTCTCGTTCTTACTGTACTCCGATCCTGCATGTCTACAGTTTATTTTTAATATGTGCTTCTCCACATTATCAAGTCCACTGGCTGTATTAAAATTTTGAGGAACATTAAATTTTTCATCGCATATATGATATGAGATCCTAATTTCTATGTTTTGAGTCCATACGGAAGACATAGTTCGAACGGCATTGGATTATGCTTGCATCAGTTAGATGGTTACTTTTAGTTTGTAATCTGTTTACACAAGAGCCTTATTTTTATCTCTTTTGAAAATTGAGCAGCAAAATCTGGTATTCTGTGCCAACAAAAATTCATTAGTAACATAGTTTGGTTAAATGTGTTTCAATATGTTGATAGAGAAATTATTTTGCAGGTGGCAAGTATCGTCAGCTCAAGCATACAACCTCTTCAAATGATAGGACTGCTGGTAGATTTTGTCTGCATGTGATTTATTTGCACTTTGTGTGTTTCCCATGTATCTGAATTTAATGTTCAAATTATTTTTAGAAAACTATGGAGGAAAAATTTGGTCTAGAAGAACAACTACCATGGGCTCAGTCTAATATAGAAAAAGGTTTCAAGGGTAAGTTTGTGGAAGTTTATTTCGTGAAGTTCTTCGGTGTTCATTGTCGTAATTATGCGTGGAAACAATATACCGTTCAAAGGAATATTGAGTACACTATACAGTAACACTGCTCGTGGCACTGAATCAATATTAGAAAGTGCAATCCAGCAATTGTAATGGTTCTCAATTTTGCATCTGTTGTGTATTACAGCTCTTGAGAAACTACTGCAGGGCTTTTCTGGCCGATATGCCTTTGGAGATGAAGTATATATGGTATGTTTCAGAATATTCTCGATCCTATTGCTACGGATAATGATTTAGATTTCATCTTTTTTTAGTACAACAAATACGGAGACGAGGGATTGAACCTCCATCCTCCAGGAGATGAATAAGGTTCCAACCATTGAGCTAAGCTTATGTTGGCGATTCTCCATCTTATTTTAGAATTAAGTTTTACACGTGTAATTTTCCTAATTTGTTATACCTTTTTTGGAATTTTCATCATCATGGTTTTAATCTTGTCTTGCCACATGCAGGGTGATGTATTTTTGGCCCCTCAGATTTCAGTTGCTATTGGGAGGTACAACATTGACATGGTATCGTATTTTCTCCACAATTATGACATTCTGTTTTAACTGCACACCTTTCTTTTAAAGCCTGGCATAGACTATGCAATGTTTCTTTAAAAAAAATAGAGAATACGGATTGCCTGCAGCTGGGGCACCCCTTTTTAAATGACTAAATTACCAAGTAATTTCATGAGATTAAGATTACGAAATTTACTAATTAGTTTTTGTAAGAAAAAAACCACTTAAACCATATTATTTTCCTATCAAGTTTTTGAAAATTTTCAATTACCTTTATAAAAAAAAATGAAGAAACAAAGCACCAAGCACATTGTTTGTTAATTAAAACAATTTGATAGCACCTTCTCGGCACAGAGGTATTAGAGGTTGAAAATTTGAAGTTCTAATATAAATTTTCTTGTGTGCGCTTTGCAGAAATCGTTAGGTATGTAAATTGTAATGTAACCACCTCCATCTCTAGATGTAACTTAGTTTCGAGCCAACATGAACTTAGCTCAACGGTATTGACACCTATCTCACAACCATTTCGAATCCCCCTATCCCACTTGTACTAAAAAAACAAAAACAAAAGCAAACTTTGTTTCCAAATATTAATATCTTAGCTGGGCCTTGCATTTGCAGCCACACATGCATTTACACTTTCTTTCCATTCTCTCTCCCGTATAACAAATTTCCGCTGTTCCATGCTGCAGTCCAAGTTTCCTGTTCTAGCGAGGATTTACGAAGCTTACAAGACGTTACCGGAATTCCAAGCCTCGTCACCAGAAAGGCAACCCGACGCCTTTCATTAGGTGACTTGTTGTCTCAATATTTTACTAATACATCACAACTTCCACTTCATATTAGTTCCTTGCGTGAAATAAATGGACGGAGAAAGGAAAGAGAAAAAAAAAACATTTCTTAATTATTGAGCACTAATATTTTATCTGTTCTTCCAACAAGATATAAAGAGCTGTCAATACGAAGATGAACTCAACATGATGACTAAATTTATTGATGCTTCCGTGAACTTAATTTTTCTCCAAGTCTCTCCCATTTCCATTTAACTTCCTTTTGGATCTCTAGTGTTTATTAAAATTTGTAAAAATCTCTTTTAGGAGGTAATTGGTGACGAAAGATTTAACTTCTTGATGTAGTAGGAAATGCGACTCTGGTATCAACAGCCCGTCTAGCTCAGTTGGTAGAGCGCAAGGCTCTTAACCTTGTGGTCGTGGGTTCGAGCCCCACGGTGGGCGAAGGTAATTTTTTTCCCTTTTTCTTACCATATTTGATTCTGGCCTTTTTTAATAGTATAAATATAACAACGATAATTTATTATTTAGAAAAGGAACGTAATTTAGTGGAACGGTAGAGGTCGAGTCATGGGACTTTTTTAAAACCGTCGGAATAAGTCTTGGAAAGAACCGCTGGATTTTGGTCCTCTTTGCAAAGATTTTTTTTCCCAATGCTGTTCTGGTTTACTGATTT

mRNA sequence

ATGTACTCCGAAGCTCAAGCTCAGCAGAAAAGCTCTGACGATTCGTCGACGTCTTCGAAGCTGGTTCTTTATTCTTTCTGGCAGAGCTCCTGCTCGTGGCGTGTTCGCTTTGCGTTGAATCTCAAAGGGCTTTCTTACGAGTACAGAGCAGTAAACCTTGGGAGAGGAGAACACTTAAGTCCAGATTTTGAGCGCCTAAATCCACTTAAATATGTTCCTATATTAGTAGATGGTCCAGTGGTAGTTTCGGACTCTTACGCGATATTTCTGTATTTGGAAGAGAAGTATCCACAGAAGGCGCTGCTGCCTGCTGATCTTCATTCAAAATGTCTGAATCTGCAAGTGGCAAGTATCGTCAGCTCAAGCATACAACCTCTTCAAATGATAGGACTGCTGAAAACTATGGAGGAAAAATTTGGTCTAGAAGAACAACTACCATGGGCTCAGTCTAATATAGAAAAAGGTTTCAAGGCTCTTGAGAAACTACTGCAGGGCTTTTCTGGCCGATATGCCTTTGGAGATGAAGTATATATGGGTGATGTATTTTTGGCCCCTCAGATTTCAGTTGCTATTGGGAGGTACAACATTGACATGTCCAAGTTTCCTGTTCTAGCGAGGATTTACGAAGCTTACAAGACGTTACCGGAATTCCAAGCCTCGTCACCAGAAAGGCAACCCGACGCCTTTCATTAG

Coding sequence (CDS)

ATGTACTCCGAAGCTCAAGCTCAGCAGAAAAGCTCTGACGATTCGTCGACGTCTTCGAAGCTGGTTCTTTATTCTTTCTGGCAGAGCTCCTGCTCGTGGCGTGTTCGCTTTGCGTTGAATCTCAAAGGGCTTTCTTACGAGTACAGAGCAGTAAACCTTGGGAGAGGAGAACACTTAAGTCCAGATTTTGAGCGCCTAAATCCACTTAAATATGTTCCTATATTAGTAGATGGTCCAGTGGTAGTTTCGGACTCTTACGCGATATTTCTGTATTTGGAAGAGAAGTATCCACAGAAGGCGCTGCTGCCTGCTGATCTTCATTCAAAATGTCTGAATCTGCAAGTGGCAAGTATCGTCAGCTCAAGCATACAACCTCTTCAAATGATAGGACTGCTGAAAACTATGGAGGAAAAATTTGGTCTAGAAGAACAACTACCATGGGCTCAGTCTAATATAGAAAAAGGTTTCAAGGCTCTTGAGAAACTACTGCAGGGCTTTTCTGGCCGATATGCCTTTGGAGATGAAGTATATATGGGTGATGTATTTTTGGCCCCTCAGATTTCAGTTGCTATTGGGAGGTACAACATTGACATGTCCAAGTTTCCTGTTCTAGCGAGGATTTACGAAGCTTACAAGACGTTACCGGAATTCCAAGCCTCGTCACCAGAAAGGCAACCCGACGCCTTTCATTAG

Protein sequence

MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH

Homology

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BLAST of MC05g_new0026.1 vs. ExPASy Swiss-Prot
Match: P57108 (Glutathione S-transferase zeta class OS=Euphorbia esula OX=3993 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 6.0e-70
Identity = 126/218 (57.80%), Postives = 168/218 (77.06%), Query Frame = 0


Query: 11  SSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLK 70
           +S +     KL LYS+++SSCS+RVR ALNLKGL YEY  VNL +GE  +P+F ++NP+ 
Sbjct: 2   ASVEQPNKPKLKLYSYFRSSCSFRVRIALNLKGLDYEYVPVNLLKGEQFTPEFLKINPIG 61

Query: 71  YVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIG 130
           YVP LVDG  V+SDS+AI +YLEEKYP+  +LPAD+H K +N Q A+IVSSSIQPLQ + 
Sbjct: 62  YVPALVDGEDVISDSFAILMYLEEKYPEHPILPADIHKKAINYQAANIVSSSIQPLQNLA 121

Query: 131 LLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVA 190
           +L  + EK   +E++PW Q +I KGF ALEKLLQG +GR+A GDEVY+ D+FL PQI  A
Sbjct: 122 VLNFIGEKVSPDEKVPWVQRHISKGFAALEKLLQGHAGRFATGDEVYLADLFLEPQIHAA 181

Query: 191 IGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDA 229
           I R+N+DM++FP+L R++EAY  LPEFQ + P++QPD+
Sbjct: 182 ITRFNVDMTQFPLLLRLHEAYSQLPEFQNAMPDKQPDS 219

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BLAST of MC05g_new0026.1 vs. ExPASy Swiss-Prot
Match: Q9ZVQ3 (Glutathione S-transferase Z1 OS=Arabidopsis thaliana OX=3702 GN=GSTZ1 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.0e-61
Identity = 114/214 (53.27%), Postives = 153/214 (71.50%), Query Frame = 0


Query: 15  SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPI 74
           +S   KL LYS+W+SSC+ RVR AL LKGL YEY  VNL +G+    DF+++NP+  VP 
Sbjct: 3   NSGEEKLKLYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPA 62

Query: 75  LVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKT 134
           LVDG VV++DS+AI +YL+EKYP+  LLP DLH + +N Q  SIV S IQP Q + +++ 
Sbjct: 63  LVDGDVVINDSFAIIMYLDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRY 122

Query: 135 MEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRY 194
           +EEK  +EE+  W  + I KGF ALEKLL   +G++A GDE+Y+ D+FLAPQI  AI R+
Sbjct: 123 IEEKINVEEKTAWVNNAITKGFTALEKLLVNCAGKHATGDEIYLADLFLAPQIHGAINRF 182

Query: 195 NIDMSKFPVLARIYEAYKTLPEFQASSPERQPDA 229
            I+M  +P LA+ YE+Y  LP FQ + PE+QPDA
Sbjct: 183 QINMEPYPTLAKCYESYNELPAFQNALPEKQPDA 216

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BLAST of MC05g_new0026.1 vs. ExPASy Swiss-Prot
Match: Q9ZVQ4 (Glutathione S-transferase Z2 OS=Arabidopsis thaliana OX=3702 GN=GSTZ2 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.8e-61
Identity = 113/209 (54.07%), Postives = 149/209 (71.29%), Query Frame = 0


Query: 19  SKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPILVDG 78
           +KL LYS+W+SSC+ RVR AL LKGL YEY  VNL +G+    DF+++NP+  VP LVDG
Sbjct: 10  AKLKLYSYWRSSCAHRVRIALTLKGLDYEYIPVNLLKGDQSDSDFKKINPMGTVPALVDG 69

Query: 79  PVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKTMEEK 138
            VV++DS+AI +YL++KYP+  LLP+D H + +N Q  SIV S IQP Q + L + +E+K
Sbjct: 70  DVVINDSFAIIMYLDDKYPEPPLLPSDYHKRAVNYQATSIVMSGIQPHQNMALFRYLEDK 129

Query: 139 FGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRYNIDM 198
              EE+  W  + I KGF ALEKLL   +G+YA GDEVY+ D+FLAPQI  A  R++I+M
Sbjct: 130 INAEEKTAWITNAITKGFTALEKLLVSCAGKYATGDEVYLADLFLAPQIHAAFNRFHINM 189

Query: 199 SKFPVLARIYEAYKTLPEFQASSPERQPD 228
             FP LAR YE+Y  LP FQ + PE+QPD
Sbjct: 190 EPFPTLARFYESYNELPAFQNAVPEKQPD 218

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BLAST of MC05g_new0026.1 vs. ExPASy Swiss-Prot
Match: O04437 (Glutathione S-transferase OS=Triticum aestivum OX=4565 GN=GSTZ1 PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 6.7e-61
Identity = 115/211 (54.50%), Postives = 154/211 (72.99%), Query Frame = 0


Query: 18  SSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPILVD 77
           ++K +LY  W SSCS RVR ALNLKG+ YEY+AVN        PD+E++NP+KY+P LVD
Sbjct: 3   TAKPILYGAWISSCSHRVRIALNLKGVDYEYKAVN----PRTDPDYEKINPIKYIPALVD 62

Query: 78  GPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKTMEE 137
           G  V+SDS AI LYLE+KYPQ  L+P D+ +K L+LQ+A+IV SSIQPLQ  G++   E 
Sbjct: 63  GDFVLSDSLAIMLYLEDKYPQHPLVPKDIKTKGLDLQIANIVCSSIQPLQGYGVIGLHEG 122

Query: 138 KFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRYNID 197
           +   +E L   Q  I+KGF+A+EKLL G   +Y  GDEV++GDV LAPQI  AI R+ ID
Sbjct: 123 RLSPDESLEVVQRYIDKGFRAIEKLLDGCDSKYCVGDEVHLGDVCLAPQIHAAINRFQID 182

Query: 198 MSKFPVLARIYEAYKTLPEFQASSPERQPDA 229
           M+K+P+L+R+++AY  +P FQA+ P+ QPDA
Sbjct: 183 MTKYPILSRLHDAYMKIPAFQAALPQNQPDA 209

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BLAST of MC05g_new0026.1 vs. ExPASy Swiss-Prot
Match: P28342 (Glutathione S-transferase 1 OS=Dianthus caryophyllus OX=3570 GN=GST1 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-60
Identity = 112/204 (54.90%), Postives = 156/204 (76.47%), Query Frame = 0


Query: 15  SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPI 74
           SS + K+ LYSF  SSC+WRVR AL+LKGL +EY+AV+L +GEHL+P+F +LNPL YVP+
Sbjct: 3   SSETQKMQLYSFSLSSCAWRVRIALHLKGLDFEYKAVDLFKGEHLTPEFLKLNPLGYVPV 62

Query: 75  LVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKT 134
           LV G +V++DS AI +YLEEK+P+  LLP DL  + LN Q A+IV+S+IQPLQ + +L  
Sbjct: 63  LVHGDIVIADSLAIIMYLEEKFPENPLLPQDLQKRALNYQAANIVTSNIQPLQNLAVLNY 122

Query: 135 MEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRY 194
           +EEK G +E+L WA+ +I+KGF ALEKLL+G +G+YA GDEV + D+FLAPQI  +I  +
Sbjct: 123 IEEKLGSDEKLSWAKHHIKKGFSALEKLLKGHAGKYATGDEVGLADLFLAPQIIASITGF 182

Query: 195 NIDMSKFPVLARIYEAYKTLPEFQ 219
            +DM++FP+L  + +AY     F+
Sbjct: 183 GMDMAEFPLLKSLNDAYLKYQHFR 206

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BLAST of MC05g_new0026.1 vs. NCBI nr
Match: XP_022147012.1 (glutathione S-transferase zeta class-like isoform X1 [Momordica charantia] >XP_022147013.1 glutathione S-transferase zeta class-like isoform X1 [Momordica charantia] >XP_022147014.1 glutathione S-transferase zeta class-like isoform X1 [Momordica charantia])

HSP 1 Score: 459 bits (1181), Expect = 6.07e-163
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60
           MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS
Sbjct: 1   MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60

Query: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120
           PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS
Sbjct: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120

Query: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180
           SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD
Sbjct: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180

Query: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH
Sbjct: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230

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BLAST of MC05g_new0026.1 vs. NCBI nr
Match: XP_022147015.1 (glutathione S-transferase zeta class-like isoform X2 [Momordica charantia])

HSP 1 Score: 453 bits (1165), Expect = 1.61e-160
Identity = 229/230 (99.57%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60
           MYSEAQAQ KSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS
Sbjct: 1   MYSEAQAQ-KSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60

Query: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120
           PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS
Sbjct: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120

Query: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180
           SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD
Sbjct: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180

Query: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH
Sbjct: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 229

[Close]

BLAST of MC05g_new0026.1 vs. NCBI nr
Match: XP_022974178.1 (glutathione S-transferase zeta class-like [Cucurbita maxima] >XP_022974180.1 glutathione S-transferase zeta class-like [Cucurbita maxima])

HSP 1 Score: 392 bits (1006), Expect = 2.67e-136
Identity = 196/227 (86.34%), Postives = 207/227 (91.19%), Query Frame = 0


Query: 4   EAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDF 63
           E +AQ +SSD SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGE LSPDF
Sbjct: 2   EFEAQAQSSDGSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEQLSPDF 61

Query: 64  ERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSI 123
           ERLNPLKYVP+LVDG VVVSDSYAI LYLEEKYPQKALLPADLH K LNLQVASIVSSSI
Sbjct: 62  ERLNPLKYVPVLVDGAVVVSDSYAILLYLEEKYPQKALLPADLHLKSLNLQVASIVSSSI 121

Query: 124 QPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFL 183
           QPL M+GLLKT+EEKFG EEQLPWAQ+NIE GF ALEKLL+ FSGRYA G+EVYM DVFL
Sbjct: 122 QPLIMLGLLKTIEEKFGPEEQLPWAQTNIENGFNALEKLLKNFSGRYALGEEVYMADVFL 181

Query: 184 APQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           APQ+SVA GR+NID SKFP LARIYE+YK LPEFQASSPERQPDA H
Sbjct: 182 APQVSVAFGRFNIDTSKFPALARIYESYKMLPEFQASSPERQPDALH 228

[Close]

BLAST of MC05g_new0026.1 vs. NCBI nr
Match: KAG6597197.1 (hypothetical protein SDJN03_10377, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 391 bits (1004), Expect = 5.39e-136
Identity = 198/227 (87.22%), Postives = 208/227 (91.63%), Query Frame = 0


Query: 4   EAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDF 63
           EAQAQ  SSD SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGE LSPDF
Sbjct: 4   EAQAQ--SSDGSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEQLSPDF 63

Query: 64  ERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSI 123
           ERLNPLKYVP+LVDG VVVSDS+AI LYLEEKYPQKALLPADLH K LNLQVASIVSSSI
Sbjct: 64  ERLNPLKYVPVLVDGAVVVSDSFAILLYLEEKYPQKALLPADLHLKSLNLQVASIVSSSI 123

Query: 124 QPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFL 183
           QPL M+GLLKT+EEKFG EEQLPWAQ+NIEKGF ALEKLL+ FSGRYA G+EVYM DVFL
Sbjct: 124 QPLIMLGLLKTIEEKFGPEEQLPWAQTNIEKGFNALEKLLKNFSGRYALGEEVYMADVFL 183

Query: 184 APQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           APQ+SVA GR+NID SKFP LARIYE+YK LPEFQASSPERQPDA H
Sbjct: 184 APQVSVAFGRFNIDSSKFPTLARIYESYKMLPEFQASSPERQPDALH 228

[Close]

BLAST of MC05g_new0026.1 vs. NCBI nr
Match: XP_023538877.1 (glutathione S-transferase zeta class-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023538878.1 glutathione S-transferase zeta class-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389 bits (1000), Expect = 4.32e-135
Identity = 198/227 (87.22%), Postives = 207/227 (91.19%), Query Frame = 0


Query: 4   EAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDF 63
           EAQAQ  SSD SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGE LSPDF
Sbjct: 23  EAQAQ--SSDGSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEQLSPDF 82

Query: 64  ERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSI 123
           ERLNPLKYVP+LVDG VVVSDSYAI LYLEEKYPQKALLPADL  K LNLQVASIVSSSI
Sbjct: 83  ERLNPLKYVPVLVDGAVVVSDSYAILLYLEEKYPQKALLPADLRLKSLNLQVASIVSSSI 142

Query: 124 QPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFL 183
           QPL M+GLLKT+EEKFG EEQLPWAQ+NIEKGF ALEKLL+ FSGRYA G+EVYM DVFL
Sbjct: 143 QPLIMLGLLKTIEEKFGPEEQLPWAQTNIEKGFNALEKLLKNFSGRYALGEEVYMADVFL 202

Query: 184 APQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           APQ+SVA GR+NID SKFP LARIYE+YK LPEFQASSPERQPDA H
Sbjct: 203 APQVSVAFGRFNIDTSKFPTLARIYESYKMLPEFQASSPERQPDALH 247

[Close]

BLAST of MC05g_new0026.1 vs. ExPASy TrEMBL
Match: A0A6J1D039 (Glutathione transferase OS=Momordica charantia OX=3673 GN=LOC111016056 PE=3 SV=1)

HSP 1 Score: 459 bits (1181), Expect = 2.94e-163
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60
           MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS
Sbjct: 1   MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60

Query: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120
           PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS
Sbjct: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120

Query: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180
           SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD
Sbjct: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180

Query: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH
Sbjct: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230

[Close]

BLAST of MC05g_new0026.1 vs. ExPASy TrEMBL
Match: A0A6J1CYY8 (Glutathione transferase OS=Momordica charantia OX=3673 GN=LOC111016056 PE=3 SV=1)

HSP 1 Score: 453 bits (1165), Expect = 7.79e-161
Identity = 229/230 (99.57%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MYSEAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60
           MYSEAQAQ KSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS
Sbjct: 1   MYSEAQAQ-KSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS 60

Query: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120
           PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS
Sbjct: 61  PDFERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVS 120

Query: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180
           SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD
Sbjct: 121 SSIQPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGD 180

Query: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH
Sbjct: 181 VFLAPQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 229

[Close]

BLAST of MC05g_new0026.1 vs. ExPASy TrEMBL
Match: A0A6J1IDA8 (Glutathione transferase OS=Cucurbita maxima OX=3661 GN=LOC111472763 PE=3 SV=1)

HSP 1 Score: 392 bits (1006), Expect = 1.29e-136
Identity = 196/227 (86.34%), Postives = 207/227 (91.19%), Query Frame = 0


Query: 4   EAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDF 63
           E +AQ +SSD SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGE LSPDF
Sbjct: 2   EFEAQAQSSDGSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEQLSPDF 61

Query: 64  ERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSI 123
           ERLNPLKYVP+LVDG VVVSDSYAI LYLEEKYPQKALLPADLH K LNLQVASIVSSSI
Sbjct: 62  ERLNPLKYVPVLVDGAVVVSDSYAILLYLEEKYPQKALLPADLHLKSLNLQVASIVSSSI 121

Query: 124 QPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFL 183
           QPL M+GLLKT+EEKFG EEQLPWAQ+NIE GF ALEKLL+ FSGRYA G+EVYM DVFL
Sbjct: 122 QPLIMLGLLKTIEEKFGPEEQLPWAQTNIENGFNALEKLLKNFSGRYALGEEVYMADVFL 181

Query: 184 APQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           APQ+SVA GR+NID SKFP LARIYE+YK LPEFQASSPERQPDA H
Sbjct: 182 APQVSVAFGRFNIDTSKFPALARIYESYKMLPEFQASSPERQPDALH 228

[Close]

BLAST of MC05g_new0026.1 vs. ExPASy TrEMBL
Match: A0A6J1F3M3 (Glutathione transferase OS=Cucurbita moschata OX=3662 GN=LOC111441820 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 2.14e-135
Identity = 197/227 (86.78%), Postives = 207/227 (91.19%), Query Frame = 0


Query: 4   EAQAQQKSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDF 63
           EAQAQ  SSD SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGE LSPDF
Sbjct: 4   EAQAQ--SSDGSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEQLSPDF 63

Query: 64  ERLNPLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSI 123
           ERLNPLKYVP+LVDG VVVSDS+AI LYLEEKYPQKALLPADLH K LNLQVASIVSSSI
Sbjct: 64  ERLNPLKYVPVLVDGAVVVSDSFAILLYLEEKYPQKALLPADLHLKSLNLQVASIVSSSI 123

Query: 124 QPLQMIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFL 183
           QPL M+GLLKT+EEKFG EEQLPWAQ+NIEKGF ALEKLL+ FS RYA G+EVYM DVFL
Sbjct: 124 QPLIMLGLLKTIEEKFGPEEQLPWAQTNIEKGFNALEKLLKNFSARYALGEEVYMADVFL 183

Query: 184 APQISVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           APQ+SVA GR+NID SKFP LARIYE+YK LPEFQASSPERQPDA H
Sbjct: 184 APQVSVAFGRFNIDSSKFPTLARIYESYKMLPEFQASSPERQPDALH 228

[Close]

BLAST of MC05g_new0026.1 vs. ExPASy TrEMBL
Match: A0A1S3AVD8 (Glutathione transferase OS=Cucumis melo OX=3656 GN=LOC103483352 PE=3 SV=1)

HSP 1 Score: 357 bits (916), Expect = 6.88e-123
Identity = 181/221 (81.90%), Postives = 194/221 (87.78%), Query Frame = 0


Query: 10  KSSDDSSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPL 69
           K SD SSTSS LVLYSFW+SSCSWRVRFALNLKGLSYEYRAV+LGR E L+PDFERLNPL
Sbjct: 9   KCSDGSSTSSNLVLYSFWRSSCSWRVRFALNLKGLSYEYRAVDLGRREQLNPDFERLNPL 68

Query: 70  KYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMI 129
           KYVP+LVDGPVVVSDSYAI LYLEEKYPQKALLP DL  K L+LQVASIVSSSIQPL M+
Sbjct: 69  KYVPVLVDGPVVVSDSYAILLYLEEKYPQKALLPTDLRLKSLHLQVASIVSSSIQPLIML 128

Query: 130 GLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISV 189
            LLKT+ EKFG EE LPWAQS +EKGF ALEKLL+ FSG+YA GDEV+M DVFLAPQIS 
Sbjct: 129 ELLKTIGEKFGPEEPLPWAQSTLEKGFNALEKLLKDFSGQYALGDEVHMADVFLAPQISS 188

Query: 190 AIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDAFH 230
           AI  + IDMSKFP LARIYE+YKTLPEFQASSPERQ DA H
Sbjct: 189 AIENFGIDMSKFPTLARIYESYKTLPEFQASSPERQLDALH 229

[Close]

BLAST of MC05g_new0026.1 vs. TAIR 10
Match: AT2G02390.1 (glutathione S-transferase zeta 1 )

HSP 1 Score: 238.0 bits (606), Expect = 7.3e-63
Identity = 114/214 (53.27%), Postives = 153/214 (71.50%), Query Frame = 0


Query: 15  SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPI 74
           +S   KL LYS+W+SSC+ RVR AL LKGL YEY  VNL +G+    DF+++NP+  VP 
Sbjct: 3   NSGEEKLKLYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPA 62

Query: 75  LVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKT 134
           LVDG VV++DS+AI +YL+EKYP+  LLP DLH + +N Q  SIV S IQP Q + +++ 
Sbjct: 63  LVDGDVVINDSFAIIMYLDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRY 122

Query: 135 MEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRY 194
           +EEK  +EE+  W  + I KGF ALEKLL   +G++A GDE+Y+ D+FLAPQI  AI R+
Sbjct: 123 IEEKINVEEKTAWVNNAITKGFTALEKLLVNCAGKHATGDEIYLADLFLAPQIHGAINRF 182

Query: 195 NIDMSKFPVLARIYEAYKTLPEFQASSPERQPDA 229
            I+M  +P LA+ YE+Y  LP FQ + PE+QPDA
Sbjct: 183 QINMEPYPTLAKCYESYNELPAFQNALPEKQPDA 216

[Close]

BLAST of MC05g_new0026.1 vs. TAIR 10
Match: AT2G02380.1 (glutathione S-transferase (class zeta) 2 )

HSP 1 Score: 237.3 bits (604), Expect = 1.3e-62
Identity = 113/209 (54.07%), Postives = 149/209 (71.29%), Query Frame = 0


Query: 19  SKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPILVDG 78
           +KL LYS+W+SSC+ RVR AL LKGL YEY  VNL +G+    DF+++NP+  VP LVDG
Sbjct: 10  AKLKLYSYWRSSCAHRVRIALTLKGLDYEYIPVNLLKGDQSDSDFKKINPMGTVPALVDG 69

Query: 79  PVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKTMEEK 138
            VV++DS+AI +YL++KYP+  LLP+D H + +N Q  SIV S IQP Q + L + +E+K
Sbjct: 70  DVVINDSFAIIMYLDDKYPEPPLLPSDYHKRAVNYQATSIVMSGIQPHQNMALFRYLEDK 129

Query: 139 FGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRYNIDM 198
              EE+  W  + I KGF ALEKLL   +G+YA GDEVY+ D+FLAPQI  A  R++I+M
Sbjct: 130 INAEEKTAWITNAITKGFTALEKLLVSCAGKYATGDEVYLADLFLAPQIHAAFNRFHINM 189

Query: 199 SKFPVLARIYEAYKTLPEFQASSPERQPD 228
             FP LAR YE+Y  LP FQ + PE+QPD
Sbjct: 190 EPFPTLARFYESYNELPAFQNAVPEKQPD 218

[Close]

BLAST of MC05g_new0026.1 vs. TAIR 10
Match: AT2G02390.3 (glutathione S-transferase zeta 1 )

HSP 1 Score: 231.1 bits (588), Expect = 9.0e-61
Identity = 114/221 (51.58%), Postives = 153/221 (69.23%), Query Frame = 0


Query: 15  SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLS-------PDFERLN 74
           +S   KL LYS+W+SSC+ RVR AL LKGL YEY  VNL +G+           DF+++N
Sbjct: 3   NSGEEKLKLYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKIN 62

Query: 75  PLKYVPILVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQ 134
           P+  VP LVDG VV++DS+AI +YL+EKYP+  LLP DLH + +N Q  SIV S IQP Q
Sbjct: 63  PMGTVPALVDGDVVINDSFAIIMYLDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQ 122

Query: 135 MIGLLKTMEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQI 194
            + +++ +EEK  +EE+  W  + I KGF ALEKLL   +G++A GDE+Y+ D+FLAPQI
Sbjct: 123 NLAVIRYIEEKINVEEKTAWVNNAITKGFTALEKLLVNCAGKHATGDEIYLADLFLAPQI 182

Query: 195 SVAIGRYNIDMSKFPVLARIYEAYKTLPEFQASSPERQPDA 229
             AI R+ I+M  +P LA+ YE+Y  LP FQ + PE+QPDA
Sbjct: 183 HGAINRFQINMEPYPTLAKCYESYNELPAFQNALPEKQPDA 223

[Close]

BLAST of MC05g_new0026.1 vs. TAIR 10
Match: AT2G02390.2 (glutathione S-transferase zeta 1 )

HSP 1 Score: 203.8 bits (517), Expect = 1.5e-52
Identity = 99/187 (52.94%), Postives = 133/187 (71.12%), Query Frame = 0


Query: 15  SSTSSKLVLYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPLKYVPI 74
           +S   KL LYS+W+SSC+ RVR AL LKGL YEY  VNL +G+    DF+++NP+  VP 
Sbjct: 3   NSGEEKLKLYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPA 62

Query: 75  LVDGPVVVSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKT 134
           LVDG VV++DS+AI +YL+EKYP+  LLP DLH + +N Q  SIV S IQP Q + +++ 
Sbjct: 63  LVDGDVVINDSFAIIMYLDEKYPEPPLLPRDLHKRAVNYQAMSIVLSGIQPHQNLAVIRY 122

Query: 135 MEEKFGLEEQLPWAQSNIEKGFKALEKLLQGFSGRYAFGDEVYMGDVFLAPQISVAIGRY 194
           +EEK  +EE+  W  + I KGF ALEKLL   +G++A GDE+Y+ D+FLAPQI  AI R+
Sbjct: 123 IEEKINVEEKTAWVNNAITKGFTALEKLLVNCAGKHATGDEIYLADLFLAPQIHGAINRF 182

Query: 195 NIDMSKF 202
            I+M  F
Sbjct: 183 QINMVLF 189

[Close]

BLAST of MC05g_new0026.1 vs. TAIR 10
Match: AT2G29450.1 (glutathione S-transferase tau 5 )

HSP 1 Score: 62.0 bits (149), Expect = 7.2e-10
Identity = 40/116 (34.48%), Postives = 58/116 (50.00%), Query Frame = 0


Query: 23  LYSFWQSSCSWRVRFALNLKGLSYEYRAVNLGRGEHLSPDFERLNPL-KYVPILVDGPVV 82
           L   W S  S RV  AL LKG+ YEY    L   E+ SP    LNP+ K VP+LV     
Sbjct: 9   LLGIWASPFSRRVEMALKLKGIPYEYVEEIL---ENKSPLLLALNPIHKKVPVLVHNGKT 68

Query: 83  VSDSYAIFLYLEEKYPQKALLPADLHSKCLNLQVASIVSSSIQPLQMIGLLKTMEE 138
           + +S+ I  Y++E +PQ  +LP D + +      A +V   I  +  I + +  E+
Sbjct: 69  ILESHVILEYIDETWPQNPILPQDPYERSKARFFAKLVDEQIMNVGFISMARADEK 121

The following BLAST results are available for this feature:

BLAST of MC05g_new0026.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P57108	6.0e-70	57.80	Glutathione S-transferase zeta class OS=Euphorbia esula OX=3993 PE=2 SV=1	[more]
Q9ZVQ3	1.0e-61	53.27	Glutathione S-transferase Z1 OS=Arabidopsis thaliana OX=3702 GN=GSTZ1 PE=1 SV=1	[more]
Q9ZVQ4	1.8e-61	54.07	Glutathione S-transferase Z2 OS=Arabidopsis thaliana OX=3702 GN=GSTZ2 PE=3 SV=1	[more]
O04437	6.7e-61	54.50	Glutathione S-transferase OS=Triticum aestivum OX=4565 GN=GSTZ1 PE=1 SV=1	[more]
P28342	1.5e-60	54.90	Glutathione S-transferase 1 OS=Dianthus caryophyllus OX=3570 GN=GST1 PE=2 SV=1	[more]

BLAST of MC05g_new0026.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147012.1	6.07e-163	100.00	glutathione S-transferase zeta class-like isoform X1 [Momordica charantia] >XP_0...	[more]
XP_022147015.1	1.61e-160	99.57	glutathione S-transferase zeta class-like isoform X2 [Momordica charantia]	[more]
XP_022974178.1	2.67e-136	86.34	glutathione S-transferase zeta class-like [Cucurbita maxima] >XP_022974180.1 glu...	[more]
KAG6597197.1	5.39e-136	87.22	hypothetical protein SDJN03_10377, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023538877.1	4.32e-135	87.22	glutathione S-transferase zeta class-like isoform X1 [Cucurbita pepo subsp. pepo...	[more]

BLAST of MC05g_new0026.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D039	2.94e-163	100.00	Glutathione transferase OS=Momordica charantia OX=3673 GN=LOC111016056 PE=3 SV=1	[more]
A0A6J1CYY8	7.79e-161	99.57	Glutathione transferase OS=Momordica charantia OX=3673 GN=LOC111016056 PE=3 SV=1	[more]
A0A6J1IDA8	1.29e-136	86.34	Glutathione transferase OS=Cucurbita maxima OX=3661 GN=LOC111472763 PE=3 SV=1	[more]
A0A6J1F3M3	2.14e-135	86.78	Glutathione transferase OS=Cucurbita moschata OX=3662 GN=LOC111441820 PE=3 SV=1	[more]
A0A1S3AVD8	6.88e-123	81.90	Glutathione transferase OS=Cucumis melo OX=3656 GN=LOC103483352 PE=3 SV=1	[more]

BLAST of MC05g_new0026.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02390.1	7.3e-63	53.27	glutathione S-transferase zeta 1	[more]
AT2G02380.1	1.3e-62	54.07	glutathione S-transferase (class zeta) 2	[more]
AT2G02390.3	9.0e-61	51.58	glutathione S-transferase zeta 1	[more]
AT2G02390.2	1.5e-52	52.94	glutathione S-transferase zeta 1	[more]
AT2G29450.1	7.2e-10	34.48	glutathione S-transferase tau 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 23..99 e-value: 3.3E-30 score: 106.1
None	No IPR available	GENE3D	1.20.1050.10		coord: 102..230 e-value: 5.5E-45 score: 154.6
None	No IPR available	SFLD	SFLDG00358	Main_(cytGST)	coord: 21..211 e-value: 8.4E-39 score: 128.0
None	No IPR available	PANTHER	PTHR42673	MALEYLACETOACETATE ISOMERASE	coord: 11..229
None	No IPR available	PANTHER	PTHR42673:SF7	GLUTATHIONE S-TRANSFERASE 2-LIKE ISOFORM X1	coord: 11..229
IPR005955	Glutathione S-transferases, class Zeta	TIGRFAM	TIGR01262	TIGR01262	coord: 23..228 e-value: 3.3E-70 score: 233.9
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF13417	GST_N_3	coord: 23..99 e-value: 1.8E-17 score: 63.4
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 19..100 score: 25.495773
IPR040079	Glutathione Transferase family	SFLD	SFLDS00019	Glutathione_Transferase_(cyto	coord: 21..211 e-value: 8.4E-39 score: 128.0
IPR010987	Glutathione S-transferase, C-terminal-like	PROSITE	PS50405	GST_CTER	coord: 105..229 score: 17.833069
IPR034333	Glutathione S-transferases, class Zeta , N-terminal	CDD	cd03042	GST_N_Zeta	coord: 21..93 e-value: 7.85185E-36 score: 119.597
IPR034330	Glutathione S-transferases, class Zeta , C-terminal	CDD	cd03191	GST_C_Zeta	coord: 106..225 e-value: 2.93061E-48 score: 153.121
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 19..103
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 101..228

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC05g_new0026	MC05g_new0026	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2124926..2125024	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2125708..2125767	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2126031..2126092	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2126286..2126361	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2126442..2126495	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2126950..2127021	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2127249..2127334	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2127417..2127473	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2127876..2127978	CDS
MC05g_new0026.1-cds	MC05g_new0026.1-cds-MC05:2128122..2128145	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC05g_new0026.1	MC05g_new0026.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009072	aromatic amino acid family metabolic process
biological_process	GO:0006749	glutathione metabolic process
biological_process	GO:0006559	L-phenylalanine catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0016034	maleylacetoacetate isomerase activity