MC05g0963.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0963.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	GTPase obg
Location	MC05: 10410653 .. 10415216 (-)
Sequence length	426
RNA-Seq Expression	MC05g0963.1
Synteny	MC05g0963.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATTCATAGCTGTTAGGCCAAATTTGGGAAATTTGAATTATGACGACTTATCATTCACAGTAGTTGACATTCCAGGTCTCATAAAGGGTGCACATGTAAATCGTGGACTCGGGCACACATTCTTGCATCACATCGAACGCATGAGGGTTCTGGCGTATGTGCTAGACTTGGTTTCTGCATTAGGCGGTAGAAAAGGGATATCCATGAGAGTATATGTACCCGATACACATTAATATAAACTATATTGTTCCTTTGAAATTTAATTTATCTTAAATTTGAACATTTCAAACTTTTTTTAATTTTTTCCAACGTAATTGATCAATGAATTTTAAAGTAAGAAGGGACTTTATGAACATACCGATTATAAGCTCCAATGATACAAAATCAATTAATTATTTTATTAATTAATTAACCTTCTTTCATAACCTAATGCTACTCCACTATAAATTAATCTGGAAGTTGCATTCTTATGTATTGTAAGAAATTTTTCTTATGCTTTTATAGATTTGAATAGCTTGAATTGCTACAGCTGACTTATGTATATGCATTTACATTTGACACTATATATACCGTTATTTTTTCTGGAACTGTTCATTCAAGTTCGATCAAATCAATGGCTCATTCATCATTAGAAGAATAAGAAGACTAACTAGGCAAAACCAAGATCAGAATGGATCAGAAGCTTGGTCACTGGTTCCACCGACTAGAATATTAGAGTGATTCAGTCGATAAAGAGGTGCGCTTGAAGTGGGGGGTGTGCTGTCCGTATTGGGCCCTTCCCCATAAGGCCCCACCGTCGGGGCATAAGCGCCCTCTTGCTACCCATATACGAGATACCGTCTTAGCCTTCCCTGACCTGGATCGCTCCCCCACCTGTTGCGTTCATGATCGGCCTACTCAACTGTGTATCGATCGAAAGAGTGTCAGGGTAAGTTTCTCTAAACAAGTTCTTCTAAAAACGAAAACATGACAACTGAAGAAACCAGAAGCGAAATAAAAATCCTTGTCAAGTAGTTGCTCTTTGGAATCTGACGATATATGCATTATTGATTTTATATACTAGTCTCCCATGTTACTGCAGGGCTTAAACTGAACAAAAAATGAAAGAATAGTGTATAATAAAGTAAGTGTTAACTATCTCAAGTCATCTTGGGGATGTAGCTCAGCTGGTAGAGCGCTCACTTAGCATCGAGAGATACGGGGATCGATACCCCGCATCTCCACTTTTTCTTGTTAATTTTACATCCCCTTGATTAAAAATTAATACAAGTTTTATGTTTAATGAACAGTACACAATTTTCAACCATGTGGGCGTGCCGGAGTGGTTATCGGGCATGACTAGAAATCATGTGGGCTTTGCCCGCGCAGGTTAGGTTTGAATCCTGCCGTTCACGGCTTATTTATTTTATATTTTTCTTGCTTCTGACTTTTTTTGTTAAGACTTTTAATTATAGACTTATCCTCCTCTTCCTTTATCCTACGCTTTTACCCCTTCAAATATATTTTTATTGCTTCTGACTTTTTTTTTAAGACTTTTAATTATAGACTTATCCTCCCCTTCCTCTGTCCTACGCTTTTACCCCTTCAAACTTCGACCACCCCACTTTGAACACCTCATCGATTTGGCTAAATTTATTTTACCTCATTTTATTTAATATTTTGGTTAAATCGAGTTGGACCAAATGACTTACCATTCTAATCTTGTTGTCAAGAACACGAAATTGAAAAGGAAAATTTGTTTCATTATTATTGGCTAGTGAGTATTATTGATAAGAATCCATTCTTTTACTTATATTTAAATTTCCTTTTTTATTCGATAAAAATTTGGTTAAAAAACACATTTGGTCTCTAAACTTTGATGTAACGATTTAGTTCCTGAACTTTCAAAAGTAACAATTTAGTCCATGTACTTTGAAATTAGTTACATTTTAGTCGTTAAAATTTTGGTTAGATTTAACGGAAGTGTGACGTGGTTGTACGTGTGTACAAATTAAATGGACAATTGTATAACCGGTTGGTTCAATCCCAAAACGATTCAAATTTGGACCGGTTGAGAACTTAAAAAATATATATATATATATATATATATATAATGAAAAATGTCCTCTCTTTCACGACTTCTTCTTAGCATATTCTCCGGCACTATCTTCCGCACCCTGAAGCCCCCAACCCTCTCTTCCTCCTCTCCGCCGCCATGATACAGAAGTTTGTCGCTGGCGTCAACCCCACCCCTGCCGACGGGGGTGGTTTAGATAAAAGATAAATTACAAATGAGCAGTGGTTCCAATTCTCCCATTTGATAACAACATAACCCTTCCCTTCCCCCTCTCATTTCCTAATGTTGGGTTTTCACTCCTCTCCTCTCCCCTTGTTCTATTTTTTTCCCTTTCAATCCCTCTTCTTCTCAATTGACCCACAGAGATGGTTTGATTGAAAATTCTCTCAATTTTGTGAGTTTTCTTTAATTTTTTTCTTGATTTTCTCAAAAGAAGAAGATCCAGAAGACGAAGGAGATTGCAGTGGCAATAGCGAAATCCTCCACTTTTAAATCCAATCATGGCGGCACCGAATCGGTACAGCGCAGAAAGAGGGGCAGACCTCGCAAGGTTGTGATGAAGCACAAAACGAAGAGAAGAACGAGGAACTTTTGCCGTCAGAAGTTGCAAAAATACCGCCGGAGAAGAAAGTTAAAACCACCGAAGATGAGCAACCGCCGCCGCCCACTGTGGAAGGAGGTGAAAAACAAGAACCAAATGAGAAGCAGAGGAAGGAGGAGGAAGAGAGATGAAAGGGAAGACATTTTCATTTTATAACATTTGTAGTTCTAAACTGGTTCAAATTTGAATCCAACCGATACGCCGTTAAAGTCGAGAGAGAAGTCTCTAAAGCCGCTATGGTCTGGGCTCTCACTCACGTCGTCCGTCCTGGCAAAGTGGGCGGGGCAATCATATGCTCGGCGAGGAAAGCGCTTGACGAACCTGGTCTCCAACCGGCACAGAAATTTGCCCAGCACAAGTCGTAAACTTCGGATCTTATGTTCCTTTCGTCAAAGGGTGACTGAGAGAGAATTTGGGCCTGGCGTGCATCCGGTTCGGTCAACAATAAGGTACAAAGCGGATTTCAAAAATGGGCTTGAAGTTCCATTATCGTTAGGTCGAAGATCTCTACTAGGTTTAGTGCACTTTGGTAGTTGCGAATGTTGCCGTCACAGCGAAGAAAGGCCGAATGGAAGGGGGCAGTCAGCTGGCTGCTTCTTGGCCACGCCCCCCTGCAACTAGATACGAGATACTTGATCTAAATTCGAAAATAGGAAATTGCATACCATTCGCCGATATACGTATAGGAACATGGGTACATGATATAGAATGTCATCCAGGTCAAAGCGCAAAGCTGAGATTGAACCAACCGCCTGCATAGTTGTCCATTTAATTTGCACACATGTACATCCACGTCACGCTTTCGTTTAATCTAACCAAAATTTTAACAGCAGGGACTAAAATATAACAAATTTAAAAGTACAGGAATTAAATTGTTACTTTTGAAAGTTCAGGGACTAAATCGTTAGTTTATTCAAAGTTCAGGGACCAAATGTATTTTTTAACCTAAAAATTTTAAGTCACTTTTTCTATTTTAAAATAGATGCAAATTTCTTCTTCTCCCCCTCCCTTTTTTGTTAAATTTTATTTCAAGGATGAAATTGGATGGTGTATTGCCTTGAAACCATGTCAACTTAAACTTATAATTTTTTTTAATGTTTTTCTATATTAATTAGGGACTTTAAAAGACAAGCTATAGGAATATGTTAGTATTATGAATATATATTTGTTTACACTAATGTATTCCTATAATTTATTCGTGGACTACCACTTATCTTAAAGTTTCAAGTTTGAGTCTTACGTTGATTGTTGCTCAATCCAGGCAACAAGACTTGATTATAAATATTGTGCCCATCATATAAAACTATGACAATTCACAAAGTTTTTTTTTAGTGCAACATGTGGGGTGGGGGATCAAACTCACCTTTAGGTTGGAGGTATATGTCAATTACCGCTGAGTTATACTCGCTTTTGCAATTCACAATTGTTATAGTTTATGTTATGAGATTTAGAATGTATTTGGGAGTGATTTTGACATGACCAAAATCACTTTTGTCATTGTCAAAATCACTTCTCTTTCTATTGATCATATTTGGAAAATTAGAAATTTGATTTTAGGAAAGCTTAAAATCACTTTAAATGAGTTTTAAGGTATCACTTTCATGGTGATTTTGGAAAATATAATTAATTCTATTTAAATCTCTTTTTTCAAAATCACTTATTCTACCATCACTCCCAAACACATCATTAGTTTCAGAGCTTGAACACCAAGTGTTCAGATCGGCTATCGTTAGGGGTGTCCAACAAGATCGACGAAGAGGGGGCCGAGCAAGTGTACAAGGAGTTAAAATCGAGAGTGCACGGGTTCCCATCTTTCGTGTCTGTGATGTTATGGAAGAGGGTGTTGCAGAACTTAAAAGCTGGTCTTAAGAACCTTGTGAATGGTGAAACATCTTATAGACTTAAA

mRNA sequence

GCATTCATAGCTGTTAGGCCAAATTTGGGAAATTTGAATTATGACGACTTATCATTCACAGTAGTTGACATTCCAGGTCTCATAAAGGGTGCACATGTAAATCGTGGACTCGGGCACACATTCTTGCATCACATCGAACGCATGAGGGTTCTGGCGTATGTGCTAGACTTGGTTTCTGCATTAGGCGGTAGAAAAGGGATATCCATGAGAGTATATAGCTTGAACACCAAGTGTTCAGATCGGCTATCGTTAGGGGTGTCCAACAAGATCGACGAAGAGGGGGCCGAGCAAGTGTACAAGGAGTTAAAATCGAGAGTGCACGGGTTCCCATCTTTCGTGTCTGTGATGTTATGGAAGAGGGTGTTGCAGAACTTAAAAGCTGGTCTTAAGAACCTTGTGAATGGTGAAACATCTTATAGACTTAAA

Coding sequence (CDS)

GCATTCATAGCTGTTAGGCCAAATTTGGGAAATTTGAATTATGACGACTTATCATTCACAGTAGTTGACATTCCAGGTCTCATAAAGGGTGCACATGTAAATCGTGGACTCGGGCACACATTCTTGCATCACATCGAACGCATGAGGGTTCTGGCGTATGTGCTAGACTTGGTTTCTGCATTAGGCGGTAGAAAAGGGATATCCATGAGAGTATATAGCTTGAACACCAAGTGTTCAGATCGGCTATCGTTAGGGGTGTCCAACAAGATCGACGAAGAGGGGGCCGAGCAAGTGTACAAGGAGTTAAAATCGAGAGTGCACGGGTTCCCATCTTTCGTGTCTGTGATGTTATGGAAGAGGGTGTTGCAGAACTTAAAAGCTGGTCTTAAGAACCTTGTGAATGGTGAAACATCTTATAGACTTAAA

Protein sequence

AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSALGGRKGISMRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPSFVSVMLWKRVLQNLKAGLKNLVNGETSYRLK

Homology

[Close]

BLAST of MC05g0963.1 vs. ExPASy Swiss-Prot
Match: F4HSD4 (Probable GTP-binding protein OBGM, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ATOBGM PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.3e-35
Identity = 86/153 (56.21%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGN+NYDD S TV DIPGLIKGAH NRGLGH FL HIER +VLAYV+DL S 
Sbjct: 334 AFTTLRPNLGNVNYDDFSMTVADIPGLIKGAHQNRGLGHNFLRHIERTKVLAYVVDLASG 393

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L G +G++         M +       SDR SL V+NKIDEEGAE+  KELK RV G   
Sbjct: 394 LDGCEGLTPWQQLRDLVMELEFHEEGLSDRSSLIVANKIDEEGAEERLKELKRRVKGVKI 453

Query: 121 FVSVMLWKRVLQNLKAGLKNLV--NGETSYRLK 143
           F    + +  +  LK GLK LV  NGE S RLK
Sbjct: 454 FPVCAVLEEGVAELKDGLKMLVDGNGEPSERLK 486

[Close]

BLAST of MC05g0963.1 vs. ExPASy Swiss-Prot
Match: Q2QZ37 (Probable GTP-binding protein OBGM, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=OBGM PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 5.8e-31
Identity = 71/144 (49.31%), Postives = 95/144 (65.97%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDD-LSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVS 60
           AF  +RPN+G+L Y+D  S  V DIPGLIKGAH NRGLGH FL HIER +VLAYVLDL +
Sbjct: 370 AFTTLRPNIGSLTYEDYFSVKVADIPGLIKGAHENRGLGHAFLRHIERTKVLAYVLDLAA 429

Query: 61  ALGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFP 120
            L GRKG+          + +       + R SL V+NKIDEEGA+++Y+ELK RV G P
Sbjct: 430 TLNGRKGVPPWEQLRDLVVELEHYQEGLTKRPSLIVANKIDEEGADEMYEELKKRVQGVP 489

Query: 121 SFVSVMLWKRVLQNLKAGLKNLVN 135
            F    + +  + +L+ GL++L++
Sbjct: 490 MFPICAILQEGVPDLRVGLRDLMD 513

[Close]

BLAST of MC05g0963.1 vs. ExPASy Swiss-Prot
Match: Q9I7M2 (GTP-binding protein 10 homolog OS=Drosophila melanogaster OX=7227 GN=CG10628 PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 4.1e-16
Identity = 46/122 (37.70%), Postives = 72/122 (59.02%), Query Frame = 0


Query: 2   FIAVRPNLGNLNYDDL-SFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDL--- 61
           F  +RP +G + Y DL S TV D+PGLI+GAH N G+GH FL HIER R+L +++D+   
Sbjct: 189 FTTIRPQIGTIEYRDLRSITVADLPGLIEGAHANFGMGHKFLKHIERTRLLVFMVDIFGF 248

Query: 62  -VSALGGRKGISMRVYSLNTKCS-------DRLSLGVSNKIDEEGAEQVYKELKSRVHGF 112
            +S     +     VY+LN +         ++ S+ + NK+D+EGA +++ ++K  V   
Sbjct: 249 QLSPKHPHRDCLANVYALNKELELYDPSLLEKPSVLLLNKMDKEGAHEIFTKVKPLVSDL 308

[Close]

BLAST of MC05g0963.1 vs. ExPASy Swiss-Prot
Match: Q9KDK0 (GTPase Obg OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=obg PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.3e-14
Identity = 46/112 (41.07%), Postives = 66/112 (58.93%), Query Frame = 0


Query: 2   FIAVRPNLGNLNYDD-LSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 61
           F  + PNLG +  DD  SF + D+PGLI+GAH   GLGH FL HIER RV+ +V+D+ SA
Sbjct: 190 FTTITPNLGVVRVDDGRSFVLADLPGLIEGAHEGIGLGHQFLRHIERTRVIVHVIDM-SA 249

Query: 62  LGGRK------GISMRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRV 107
           L GR        I+  + + N +  +R  L V+NK+D   A +  +  K ++
Sbjct: 250 LEGRDPYDDYVSINEELKAYNLRLMERPQLIVANKMDMPNAAENLERFKEKL 300

[Close]

BLAST of MC05g0963.1 vs. ExPASy Swiss-Prot
Match: Q65GM7 (GTPase Obg OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=obg PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.7e-14
Identity = 49/132 (37.12%), Postives = 76/132 (57.58%), Query Frame = 0


Query: 2   FIAVRPNLGNLNYDD-LSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 61
           F  + PNLG +  +D  SF + D+PGLI+GAH   GLGH FL HIER RV+ +V+D+ S 
Sbjct: 190 FTTLNPNLGMVETEDGRSFVMADLPGLIEGAHEGVGLGHQFLRHIERTRVIVHVIDM-SG 249

Query: 62  LGGRK------GISMRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRV-HGFPSFV 121
           L GR        I+  +   N + ++R  + V+NK+D   AE+  K  K ++   +P F 
Sbjct: 250 LEGRDPYEDYVTINKELEQYNLRLTERPQIIVANKMDMPDAEENLKAFKEKLTDDYPVFP 309

Query: 122 SVMLWKRVLQNL 126
              + ++ L++L
Sbjct: 310 ISAVTRQGLRDL 320

[Close]

BLAST of MC05g0963.1 vs. NCBI nr
Match: KAG7036927.1 (putative GTP-binding protein OBGM, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 176 bits (447), Expect = 6.66e-51
Identity = 96/150 (64.00%), Postives = 106/150 (70.67%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH+FL HIER RVLAYVLDL + 
Sbjct: 233 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAG 292

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI            +       SDR SL V+NKIDEEG EQVY+ELKSRV G P 
Sbjct: 293 LDGRKGIPPWEQLRDLVAELEHHQKGLSDRPSLVVANKIDEEGTEQVYEELKSRVQGVPI 352

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRL 141
           F    + +  +  LKAGLK+LVNGETSYRL
Sbjct: 353 FPVCAVLEEGVAELKAGLKSLVNGETSYRL 382

[Close]

BLAST of MC05g0963.1 vs. NCBI nr
Match: XP_022151583.1 (probable GTP-binding protein OBGM, mitochondrial [Momordica charantia] >XP_022151584.1 probable GTP-binding protein OBGM, mitochondrial [Momordica charantia])

HSP 1 Score: 178 bits (451), Expect = 2.17e-50
Identity = 98/151 (64.90%), Postives = 110/151 (72.85%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH FL HIER RVLAYVLDL +A
Sbjct: 356 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHAFLRHIERTRVLAYVLDLAAA 415

Query: 61  LGGRKGISM--RVYSLNTKC-------SDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L G KGI    ++  L ++        SDR SL V+NKIDEEGAEQVY+ELKSRVHG P 
Sbjct: 416 LDGNKGIPPWEQLQDLVSELEHHQKGLSDRPSLVVANKIDEEGAEQVYEELKSRVHGVPI 475

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRLK 142
           F    + +  +  LKAGLK+LVNGE  YRLK
Sbjct: 476 FPVCAVLEEGVAELKAGLKSLVNGEAFYRLK 506

[Close]

BLAST of MC05g0963.1 vs. NCBI nr
Match: XP_023522978.1 (probable GTP-binding protein OBGM, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 178 bits (451), Expect = 2.33e-50
Identity = 97/150 (64.67%), Postives = 107/150 (71.33%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH+FL HIER RVLAYVLDL +A
Sbjct: 360 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAA 419

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI            +       SDR SL V+NKIDEEG EQVY+ELKSRV G P 
Sbjct: 420 LDGRKGIPPWEQLRDLVAELEHHQKGLSDRPSLVVANKIDEEGTEQVYEELKSRVQGVPI 479

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRL 141
           F    + +  +  LKAGLK+LVNGETSYRL
Sbjct: 480 FPVCAVLEEGVAELKAGLKSLVNGETSYRL 509

[Close]

BLAST of MC05g0963.1 vs. NCBI nr
Match: XP_022997993.1 (probable GTP-binding protein OBGM, mitochondrial [Cucurbita maxima])

HSP 1 Score: 176 bits (447), Expect = 1.02e-49
Identity = 96/150 (64.00%), Postives = 106/150 (70.67%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH+FL HIER RVLAYVLDL + 
Sbjct: 367 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAG 426

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI            +       SDR SL V+NKIDEEG EQVY+ELKSRV G P 
Sbjct: 427 LDGRKGIPPWEQLRDLVAELEHHQKGLSDRPSLVVANKIDEEGTEQVYEELKSRVQGVPI 486

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRL 141
           F    + +  +  LKAGLK+LVNGETSYRL
Sbjct: 487 FPVCAVLEEGVAELKAGLKSLVNGETSYRL 516

[Close]

BLAST of MC05g0963.1 vs. NCBI nr
Match: XP_022948603.1 (probable GTP-binding protein OBGM, mitochondrial [Cucurbita moschata])

HSP 1 Score: 176 bits (447), Expect = 1.05e-49
Identity = 96/150 (64.00%), Postives = 106/150 (70.67%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH+FL HIER RVLAYVLDL + 
Sbjct: 369 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAG 428

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI            +       SDR SL V+NKIDEEG EQVY+ELKSRV G P 
Sbjct: 429 LDGRKGIPPWEQLRDLVAELEHHQKGLSDRPSLVVANKIDEEGTEQVYEELKSRVQGVPI 488

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRL 141
           F    + +  +  LKAGLK+LVNGETSYRL
Sbjct: 489 FPVCAVLEEGVAELKAGLKSLVNGETSYRL 518

[Close]

BLAST of MC05g0963.1 vs. ExPASy TrEMBL
Match: A0A6J1DCJ9 (probable GTP-binding protein OBGM, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111019488 PE=4 SV=1)

HSP 1 Score: 178 bits (451), Expect = 1.05e-50
Identity = 98/151 (64.90%), Postives = 110/151 (72.85%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH FL HIER RVLAYVLDL +A
Sbjct: 356 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHAFLRHIERTRVLAYVLDLAAA 415

Query: 61  LGGRKGISM--RVYSLNTKC-------SDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L G KGI    ++  L ++        SDR SL V+NKIDEEGAEQVY+ELKSRVHG P 
Sbjct: 416 LDGNKGIPPWEQLQDLVSELEHHQKGLSDRPSLVVANKIDEEGAEQVYEELKSRVHGVPI 475

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRLK 142
           F    + +  +  LKAGLK+LVNGE  YRLK
Sbjct: 476 FPVCAVLEEGVAELKAGLKSLVNGEAFYRLK 506

[Close]

BLAST of MC05g0963.1 vs. ExPASy TrEMBL
Match: A0A6J1KFJ2 (probable GTP-binding protein OBGM, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492781 PE=4 SV=1)

HSP 1 Score: 176 bits (447), Expect = 4.92e-50
Identity = 96/150 (64.00%), Postives = 106/150 (70.67%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH+FL HIER RVLAYVLDL + 
Sbjct: 367 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAG 426

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI            +       SDR SL V+NKIDEEG EQVY+ELKSRV G P 
Sbjct: 427 LDGRKGIPPWEQLRDLVAELEHHQKGLSDRPSLVVANKIDEEGTEQVYEELKSRVQGVPI 486

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRL 141
           F    + +  +  LKAGLK+LVNGETSYRL
Sbjct: 487 FPVCAVLEEGVAELKAGLKSLVNGETSYRL 516

[Close]

BLAST of MC05g0963.1 vs. ExPASy TrEMBL
Match: A0A6J1G9R8 (probable GTP-binding protein OBGM, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111452229 PE=4 SV=1)

HSP 1 Score: 176 bits (447), Expect = 5.09e-50
Identity = 96/150 (64.00%), Postives = 106/150 (70.67%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDDLS TV DIPGLIKGAH NRGLGH+FL HIER RVLAYVLDL + 
Sbjct: 369 AFTTLRPNLGNLNYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAG 428

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI            +       SDR SL V+NKIDEEG EQVY+ELKSRV G P 
Sbjct: 429 LDGRKGIPPWEQLRDLVAELEHHQKGLSDRPSLVVANKIDEEGTEQVYEELKSRVQGVPI 488

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRL 141
           F    + +  +  LKAGLK+LVNGETSYRL
Sbjct: 489 FPVCAVLEEGVAELKAGLKSLVNGETSYRL 518

[Close]

BLAST of MC05g0963.1 vs. ExPASy TrEMBL
Match: A0A5B7B7T5 (OBG-type G domain-containing protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_034076 PE=4 SV=1)

HSP 1 Score: 167 bits (422), Expect = 6.18e-50
Identity = 89/150 (59.33%), Postives = 103/150 (68.67%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNLNYDD S TV DIPGLI+GAH NRGLGH FL HIER +VLAYVLDL +A
Sbjct: 25  AFTTLRPNLGNLNYDDFSVTVADIPGLIRGAHANRGLGHAFLRHIERTKVLAYVLDLAAA 84

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI          + +       SDR SL V+NKIDE GA++VY+ELK RVHG P 
Sbjct: 85  LDGRKGIPPWEQLKDLILELEFYREGLSDRPSLVVANKIDEAGADEVYEELKRRVHGVPI 144

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRL 141
           F    + +  +  LKAGL+ LVNG  SYRL
Sbjct: 145 FPVCAVLEEGIPELKAGLRMLVNGGESYRL 174

[Close]

BLAST of MC05g0963.1 vs. ExPASy TrEMBL
Match: A0A0A0KWW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623700 PE=4 SV=1)

HSP 1 Score: 175 bits (444), Expect = 1.08e-49
Identity = 98/151 (64.90%), Postives = 112/151 (74.17%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGNL+YDDLS TV DIPGLIKGAH NRGLGH+FL HIER RVLAYVLDL +A
Sbjct: 354 AFTTLRPNLGNLHYDDLSITVADIPGLIKGAHENRGLGHSFLRHIERTRVLAYVLDLAAA 413

Query: 61  LGGRKGI----SMR--VYSLN---TKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L GRKGI     +R  VY L    +  SDR SL V+NKIDEEGAE+VY+ELKSRV G P 
Sbjct: 414 LDGRKGIPPWEQLRDLVYELERHQSGLSDRPSLIVANKIDEEGAEEVYEELKSRVQGVPI 473

Query: 121 FVSVMLWKRVLQNLKAGLKNLVNGETSYRLK 142
           F    + +  +  LKAGLK+LVNG+T  RLK
Sbjct: 474 FPVCAVLEEGVDELKAGLKSLVNGDTPSRLK 504

[Close]

BLAST of MC05g0963.1 vs. TAIR 10
Match: AT1G07615.1 (GTP-binding protein Obg/CgtA )

HSP 1 Score: 149.8 bits (377), Expect = 1.6e-36
Identity = 86/153 (56.21%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 1   AFIAVRPNLGNLNYDDLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLDLVSA 60
           AF  +RPNLGN+NYDD S TV DIPGLIKGAH NRGLGH FL HIER +VLAYV+DL S 
Sbjct: 334 AFTTLRPNLGNVNYDDFSMTVADIPGLIKGAHQNRGLGHNFLRHIERTKVLAYVVDLASG 393

Query: 61  LGGRKGIS---------MRVYSLNTKCSDRLSLGVSNKIDEEGAEQVYKELKSRVHGFPS 120
           L G +G++         M +       SDR SL V+NKIDEEGAE+  KELK RV G   
Sbjct: 394 LDGCEGLTPWQQLRDLVMELEFHEEGLSDRSSLIVANKIDEEGAEERLKELKRRVKGVKI 453

Query: 121 FVSVMLWKRVLQNLKAGLKNLV--NGETSYRLK 143
           F    + +  +  LK GLK LV  NGE S RLK
Sbjct: 454 FPVCAVLEEGVAELKDGLKMLVDGNGEPSERLK 486

[Close]

BLAST of MC05g0963.1 vs. TAIR 10
Match: AT5G18570.1 (GTP1/OBG family protein )

HSP 1 Score: 59.3 bits (142), Expect = 2.9e-09
Identity = 27/56 (48.21%), Postives = 37/56 (66.07%), Query Frame = 0


Query: 2   FIAVRPNLGNLNYD-DLSFTVVDIPGLIKGAHVNRGLGHTFLHHIERMRVLAYVLD 57
           F  + PNLG +++D D +  V D+PGL++GAH   GLGH FL H ER   L +V+D
Sbjct: 410 FTTLLPNLGVVSFDYDSTMVVADLPGLLEGAHRGFGLGHEFLRHTERCSALVHVVD 465

The following BLAST results are available for this feature:

BLAST of MC05g0963.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HSD4	2.3e-35	56.21	Probable GTP-binding protein OBGM, mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
Q2QZ37	5.8e-31	49.31	Probable GTP-binding protein OBGM, mitochondrial OS=Oryza sativa subsp. japonica...	[more]
Q9I7M2	4.1e-16	37.70	GTP-binding protein 10 homolog OS=Drosophila melanogaster OX=7227 GN=CG10628 PE=...	[more]
Q9KDK0	1.3e-14	41.07	GTPase Obg OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 /...	[more]
Q65GM7	1.7e-14	37.12	GTPase Obg OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NB...	[more]

BLAST of MC05g0963.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036927.1	6.66e-51	64.00	putative GTP-binding protein OBGM, mitochondrial, partial [Cucurbita argyrosperm...	[more]
XP_022151583.1	2.17e-50	64.90	probable GTP-binding protein OBGM, mitochondrial [Momordica charantia] >XP_02215...	[more]
XP_023522978.1	2.33e-50	64.67	probable GTP-binding protein OBGM, mitochondrial [Cucurbita pepo subsp. pepo]	[more]
XP_022997993.1	1.02e-49	64.00	probable GTP-binding protein OBGM, mitochondrial [Cucurbita maxima]	[more]
XP_022948603.1	1.05e-49	64.00	probable GTP-binding protein OBGM, mitochondrial [Cucurbita moschata]	[more]

BLAST of MC05g0963.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCJ9	1.05e-50	64.90	probable GTP-binding protein OBGM, mitochondrial OS=Momordica charantia OX=3673 ...	[more]
A0A6J1KFJ2	4.92e-50	64.00	probable GTP-binding protein OBGM, mitochondrial OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1G9R8	5.09e-50	64.00	probable GTP-binding protein OBGM, mitochondrial OS=Cucurbita moschata OX=3662 G...	[more]
A0A5B7B7T5	6.18e-50	59.33	OBG-type G domain-containing protein (Fragment) OS=Davidia involucrata OX=16924 ...	[more]
A0A0A0KWW4	1.08e-49	64.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G623700 PE=4 SV=1	[more]

BLAST of MC05g0963.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07615.1	1.6e-36	56.21	GTP-binding protein Obg/CgtA	[more]
AT5G18570.1	2.9e-09	48.21	GTP1/OBG family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006073	GTP binding domain	PRINTS	PR00326	GTP1OBG	coord: 1..10 score: 15.11 coord: 21..36 score: 51.51 coord: 38..56 score: 34.94
IPR006073	GTP binding domain	PFAM	PF01926	MMR_HSR1	coord: 5..61 e-value: 1.4E-7 score: 31.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..142 e-value: 2.2E-18 score: 68.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 4..116
None	No IPR available	PANTHER	PTHR11702:SF42	BNAA08G29050D PROTEIN	coord: 1..142
IPR045086	OBG-type GTPase	PANTHER	PTHR11702	DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED	coord: 1..142

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC05g0963	MC05g0963	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g0963.1-cds	MC05g0963.1-cds-MC05:10410653..10410862	CDS
MC05g0963.1-cds	MC05g0963.1-cds-MC05:10415001..10415216	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC05g0963.1	MC05g0963.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding