MC05g0300.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0300.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Aurora kinase
Location	MC05: 2250620 .. 2254229 (-)
Sequence length	1304
RNA-Seq Expression	MC05g0300.1
Synteny	MC05g0300.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAATAAATAAAACAATACAAAATAATAATAAATGACGGAAATGGTCAATGAAACGGTGCGTTTAGGATTCGACATGATTCAAGAAATTCAAATTCGTTCCGTTCGAATTTCCATCCAACGTCCCCAATTCCCTCGCTATCCTCCGCAGTCCACCCCCACATTTCTCGCTATCGGAGTTCTCCGGCGGCGAACCGATGAAATCTCAGGCGAAGGACGACAATGAAACTCCTATGCGGCAATGGTCCTTGCAAGATTTCGACGTAGGAAAGCCCCTCGGCAAAGGAAAGTTCGGCAGAGTCTATCTAGCAAGAGAAGTCAAGGTTCGTTTTCCTTCCGTTTCATTTTATGTTTGATTCTTATTGAAATATTCTACAATTTTGCATCACGCCGGATTCTTAGACCTCAGGTTCTTTGTTTTTGTATGATTCGTTTTTTGTTTGAGTGTATTTTGTTTTTGTCTACTCAATAGCTTCTAAATCTGAAAGGAATTTCGTCATTTTGAGGTTGCATGTGAATTATCCGATGGATAGATTTGTTATGAATTCGTACTTTCTCTTCTTCTTTCCCGTAAATAAAGCAGAGAGTACTAAGTCTCCTTTTCTGGAAATTATCGTAGCTACATAATGCCATCACTTTCTCAATGACCGAACGGAGTGATTCAAATTAATATTTCTTTGTATCGAATAGCGATCGTACGATTTCTCATATATACGCAACTTATAGTATTTCCAGTCCATTTTTCATTGAAATTGAGCACGAAAATCCTTTGCATTTCTCTGATTCCGATCATAAGATTGTACATCTGGATTTTGGTTTCACCTTAGGAGCTTCAATTTAATTTTTCAGAGCAAGTATATAGTGGCACTGAAGGTGATTTTCAAGGAGCAGATGGAGAAGTACAGGATTCATCATCAATTAAGGAGAGAGATGGAGATACAGACCAGTCTTCGGCACCCGAACATCCTGCGTCTCTATGGATGGTTTCATGATGCTGAACGGATTTTCTTGATATTGGAATACGCTCACCGCGGTGAGCTTTATAGGGAGCTTAGGAAAAGTGGTCATCTCAGCGAGAAGCAAGCTGCCACTGTAATTTTTCTCTCTCTATCCTTATGAATTTATATATTTTTCTCTCTATCCTTATGAATTTATTTACTTTGGACTGAAGATAACGAAGAAGTAAAGGATACAATAATATTGAGCCAGAAACAAAACTGTAGGAACTCATCTCATGAACTTCAGAATTTTCCATATTCTACTTTTCACTTTATAACTTCATATTGGACTGAGATACAGGAAACGATAATCTTGCCATTTAAATTTAGCCCTTATATATCGCTATCTAAAGGTTCTTTTATGTTGGTTTCTTGTGAATTTCAGTACATTTTAAGCCTCACCCAAGCATTGGCATACTGCCATGAGAAGCATGTGATTCACAGGGACATTAAGCCAGAAAATTTGCTTCTTGATCATGAGGTATATATTTTCCTCTTTTTGTTATTCAGCAGCTTAGAAAATGTCGGCGTTTAATTTCGTAATACCTTAGTGTTGATGGGATTAATTCTCATATTCGTTCTGGTAAATCAATGAGAAGCTCAGCCTTTCAGCCAACGGCCATCTCTCCTCTGGCTTTGTTGTTAAGCTGATTAGTTAGAGCTATAACTAATTGGTGGAGACTTAACATTGAACCGTAGCTTCTAAACGTGTGTAGCTCTCTTTGTATCCTCTGTAAGTTCGGATCGAAAGGTGTGGGAACTTCTTGATCAGCCCCCATGTCGCCACATGGGATGGACATAGAGACGTAAAAAGGGGATCCTATCAACTTGTTCTGAATTCCTTGAGCAATAAGAACTTTCTCTCTGATTTGAAAGTAGACGTAGAGCTTTGGTTGAACAGCTATAATCTGTTTGTCCATTCTATACTTGATTCTATTGATCTTTAACTCCTTTCTTGAATTTAAAACTGAATGTTTTTGGCAACTCTGCTGGTTTTGGAATAAACTTCCAATTGATTCAACACTTAGTAAACCCGTTCTTTGATCCTTGAAGTAGAACTAGAATTCCACTGCAGTTTGTAGAAGAAACAAGTCTATACTATTTATCTATTTTTCCTTTTGTGTAACCATATTTTATTTGTAGGGCCGATTGAAAATTGCAGATTTTGGATGGTCTGTACAGTCAAGAAGCAAAAGACGTACCATGTGTGGAACCCTGGATTATTTAGCCCCAGAAATGGTGGAGAATAAAGCTCACGATCATGCTGTAGATAACTGGACTCTGGGTATCCTTTGCTATGAGTTCCTTTATGGTGTGCCTCCATTCGAAGCCGAAAGTCAAAGTGACACATTTAAAAGGTTAGAAACATAACTTACGAAACAATTCAACTAGATCCTTATCCTCCACGAAGAGTCGCTACTAATTTGGTCCCTGCACTTTTATTGAAGTAACTGCTTAGTCGCTAAATTTTAGTTTATAACAGTTTAATCTATGCGCTTTCAAATCGGTAACAATTTGGCTCATAAACTTTACTACGTAACAATTTAGTCCTTGTATTTTAAAATTTATACCAATTTAGTCCCTTGTCGAAAAAAATCTCATCAAAATTAAGTGTGATTTTTATCATATAATAATTATCTTTGTAATGTATAAACATATTTAGGCCCTTATCAGTTTATAAGAAAAATTATTAAATCTTAACGAAGATTTTCACAATATAGACTAAATTGTTACAAACTTTAAACTACAAAGGCTAAATTGTTACATACTAAAGTTCAAGGATTAAATTAAAAGTATAGAAGTTAAATCGTTACTTCAACGGGAGTAAAGGACCAATAGTGTTTTTTAACCCACTTTCTTTCTTAGGTTATATATTATAAATAATCGAATCCCACTAATATTTTTAAGTCAATAGAAAGAAGTTCCTACCAGGCTATTTCTTTTTCTTAATTAACGACAACATAATCGAGATACTAAATGGTTGTTCGGGTCAAATGCAGGATAATGAAGGTGGACTTGAATTTTCCCTTGACCCCTCAGGTTTCCCCAGAAGCAAGAGATCTCATTGGCCGGGTTAGTTAAATTGTGACATTTTGTTTGGTTTTTTCTTTTTTTTTTCTTTTCTGTTTTGATAAGTGGGAGTCGGAGCTTTTTGCTCCTAGGAGTACCACGCTCACTCTGGAATTGTGTTTGGTAGTTTTAGGAATAATATGGATTTTTTTTTTTTTTGATTCTGAATATGTGAAATTTTTACTCAGCTCTTAGTGAAGGATTCCTCTAGAAGACTTTCACTAGAGAAGATAGTGGAGCACCCTTGGATAATCAAGAATGCAGATCCATCTGGTATTTGCAATAGGTAGAAACATCACTTCGTTTTTTAACTAATTGATCTTGATCATGCCTCCTCTCATTTAAATAGAGTTTGATTAAGATCTGTGTAATTTCCCAGTGTTGTAGCATTATTGATGATTTAACCATAATCTCGAAAAAAAAATGTCGCTAGTAGCTTTACTAGTAGTATTATAGGCTCTCATTCCTTGAGGCTATATGTTCTAATTCTCATTCTTTTCGATGTGACTACGTTTGAAATAGTTTTTTAAAATGTGTGTATTCA

mRNA sequence

GAAATAAATAAAACAATACAAAATAATAATAAATGACGGAAATGGTCAATGAAACGGTGCGTTTAGGATTCGACATGATTCAAGAAATTCAAATTCGTTCCGTTCGAATTTCCATCCAACGTCCCCAATTCCCTCGCTATCCTCCGCAGTCCACCCCCACATTTCTCGCTATCGGAGTTCTCCGGCGGCGAACCGATGAAATCTCAGGCGAAGGACGACAATGAAACTCCTATGCGGCAATGGTCCTTGCAAGATTTCGACGTAGGAAAGCCCCTCGGCAAAGGAAAGTTCGGCAGAGTCTATCTAGCAAGAGAAGTCAAGAGCAAGTATATAGTGGCACTGAAGGTGATTTTCAAGGAGCAGATGGAGAAGTACAGGATTCATCATCAATTAAGGAGAGAGATGGAGATACAGACCAGTCTTCGGCACCCGAACATCCTGCGTCTCTATGGATGGTTTCATGATGCTGAACGGATTTTCTTGATATTGGAATACGCTCACCGCGGTGAGCTTTATAGGGAGCTTAGGAAAAGTGGTCATCTCAGCGAGAAGCAAGCTGCCACTTACATTTTAAGCCTCACCCAAGCATTGGCATACTGCCATGAGAAGCATGTGATTCACAGGGACATTAAGCCAGAAAATTTGCTTCTTGATCATGAGGGCCGATTGAAAATTGCAGATTTTGGATGGTCTGTACAGTCAAGAAGCAAAAGACGTACCATGTGTGGAACCCTGGATTATTTAGCCCCAGAAATGGTGGAGAATAAAGCTCACGATCATGCTGTAGATAACTGGACTCTGGGTATCCTTTGCTATGAGTTCCTTTATGGTGTGCCTCCATTCGAAGCCGAAAGTCAAAGTGACACATTTAAAAGGATAATGAAGGTGGACTTGAATTTTCCCTTGACCCCTCAGGTTTCCCCAGAAGCAAGAGATCTCATTGGCCGGCTCTTAGTGAAGGATTCCTCTAGAAGACTTTCACTAGAGAAGATAGTGGAGCACCCTTGGATAATCAAGAATGCAGATCCATCTGGTATTTGCAATAGGTAGAAACATCACTTCGTTTTTTAACTAATTGATCTTGATCATGCCTCCTCTCATTTAAATAGAGTTTGATTAAGATCTGTGTAATTTCCCAGTGTTGTAGCATTATTGATGATTTAACCATAATCTCGAAAAAAAAATGTCGCTAGTAGCTTTACTAGTAGTATTATAGGCTCTCATTCCTTGAGGCTATATGTTCTAATTCTCATTCTTTTCGATGTGACTACGTTTGAAATAGTTTTTTAAAATGTGTGTATTCA

Coding sequence (CDS)

ATGAAATCTCAGGCGAAGGACGACAATGAAACTCCTATGCGGCAATGGTCCTTGCAAGATTTCGACGTAGGAAAGCCCCTCGGCAAAGGAAAGTTCGGCAGAGTCTATCTAGCAAGAGAAGTCAAGAGCAAGTATATAGTGGCACTGAAGGTGATTTTCAAGGAGCAGATGGAGAAGTACAGGATTCATCATCAATTAAGGAGAGAGATGGAGATACAGACCAGTCTTCGGCACCCGAACATCCTGCGTCTCTATGGATGGTTTCATGATGCTGAACGGATTTTCTTGATATTGGAATACGCTCACCGCGGTGAGCTTTATAGGGAGCTTAGGAAAAGTGGTCATCTCAGCGAGAAGCAAGCTGCCACTTACATTTTAAGCCTCACCCAAGCATTGGCATACTGCCATGAGAAGCATGTGATTCACAGGGACATTAAGCCAGAAAATTTGCTTCTTGATCATGAGGGCCGATTGAAAATTGCAGATTTTGGATGGTCTGTACAGTCAAGAAGCAAAAGACGTACCATGTGTGGAACCCTGGATTATTTAGCCCCAGAAATGGTGGAGAATAAAGCTCACGATCATGCTGTAGATAACTGGACTCTGGGTATCCTTTGCTATGAGTTCCTTTATGGTGTGCCTCCATTCGAAGCCGAAAGTCAAAGTGACACATTTAAAAGGATAATGAAGGTGGACTTGAATTTTCCCTTGACCCCTCAGGTTTCCCCAGAAGCAAGAGATCTCATTGGCCGGCTCTTAGTGAAGGATTCCTCTAGAAGACTTTCACTAGAGAAGATAGTGGAGCACCCTTGGATAATCAAGAATGCAGATCCATCTGGTATTTGCAATAGGTAG

Protein sequence

MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLDYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICNR

Homology

[Close]

BLAST of MC05g0300.1 vs. ExPASy Swiss-Prot
Match: O64629 (Serine/threonine-protein kinase Aurora-3 OS=Arabidopsis thaliana OX=3702 GN=AUR3 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 3.3e-134
Identity = 224/281 (79.72%), Postives = 254/281 (90.39%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           K   + D     +QWSL DF++G+PLGKGKFGRVYLARE KSKYIVALKVIFKEQ+EKY+
Sbjct: 3   KKSTESDAGNTEKQWSLADFEIGRPLGKGKFGRVYLAREAKSKYIVALKVIFKEQIEKYK 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRL+GWFHD ERIFLILEYAH GELY  L+++GHL+E+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLFGWFHDNERIFLILEYAHGGELYGVLKQNGHLTEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYI SL+QALAYCH K VIHRDIKPENLLLDHEGRLKIADFGWSVQS +KR+TMCGTLD
Sbjct: 123 ATYIASLSQALAYCHGKCVIHRDIKPENLLLDHEGRLKIADFGWSVQSSNKRKTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVEN+ HD+AVDNWTLGILCYEFLYG PPFEAESQ DTFKRI+K+DL+FPLTP V
Sbjct: 183 YLAPEMVENRDHDYAVDNWTLGILCYEFLYGNPPFEAESQKDTFKRILKIDLSFPLTPNV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGIC 283
           S EA++LI +LLVKD S+RLS+EKI++HPWI+KNADP G+C
Sbjct: 243 SEEAKNLISQLLVKDPSKRLSIEKIMQHPWIVKNADPKGVC 283

[Close]

BLAST of MC05g0300.1 vs. ExPASy Swiss-Prot
Match: Q683C9 (Serine/threonine-protein kinase Aurora-2 OS=Arabidopsis thaliana OX=3702 GN=AUR2 PE=1 SV=2)

HSP 1 Score: 394.0 bits (1011), Expect = 1.4e-108
Identity = 179/273 (65.57%), Postives = 231/273 (84.62%), Query Frame = 0


Query: 9   NETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLRR 68
           +E   ++W+  DFD+GKPLG+GKFG VYLARE +S +IVALKV+FK Q+++ ++ HQLRR
Sbjct: 7   SEAAQKRWTTSDFDIGKPLGRGKFGHVYLAREKRSDHIVALKVLFKAQLQQSQVEHQLRR 66

Query: 69  EMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSL 128
           E+EIQ+ LRHPNILRLYG+F+D +R++LILEYA RGELY+EL+K  + SE++AATY+ SL
Sbjct: 67  EVEIQSHLRHPNILRLYGYFYDQKRVYLILEYAVRGELYKELQKCKYFSERRAATYVASL 126

Query: 129 TQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLDYLAPEMV 188
            +AL YCH KHVIHRDIKPENLL+  +G LKIADFGWSV + ++RRTMCGTLDYL PEMV
Sbjct: 127 ARALIYCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMV 186

Query: 189 ENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDL 248
           E+  HD +VD W+LGILCYEFLYGVPPFEA   S+T+KRI++VDL FP  P VS  A+DL
Sbjct: 187 ESVEHDASVDIWSLGILCYEFLYGVPPFEAREHSETYKRIVQVDLKFPPKPIVSSSAKDL 246

Query: 249 IGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGI 282
           I ++LVK+S++RL+L K++EHPWI++NADPSG+
Sbjct: 247 ISQMLVKESTQRLALHKLLEHPWIVQNADPSGL 279

[Close]

BLAST of MC05g0300.1 vs. ExPASy Swiss-Prot
Match: Q9M077 (Serine/threonine-protein kinase Aurora-1 OS=Arabidopsis thaliana OX=3702 GN=AUR1 PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.8e-108
Identity = 178/268 (66.42%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 14  RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLRREMEIQ 73
           ++W+L DFD+GKPLG+GKFG VYLARE +S ++VALKV+FK Q+++ ++ HQLRRE+EIQ
Sbjct: 24  KRWTLSDFDIGKPLGRGKFGHVYLAREKRSNHVVALKVLFKSQLQQSQVEHQLRREVEIQ 83

Query: 74  TSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSLTQALA 133
           + LRHPNILRLYG+F+D +R++LILEYA RGELY++L+K  + SE++AATY+ SL +AL 
Sbjct: 84  SHLRHPNILRLYGYFYDQKRVYLILEYAARGELYKDLQKCKYFSERRAATYVASLARALI 143

Query: 134 YCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLDYLAPEMVENKAH 193
           YCH KHVIHRDIKPENLL+  +G LKIADFGWSV + ++RRTMCGTLDYL PEMVE+  H
Sbjct: 144 YCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMVESVEH 203

Query: 194 DHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDLIGRLL 253
           D +VD W+LGILCYEFLYGVPPFEA   SDT++RI++VDL FP  P +S  A+DLI ++L
Sbjct: 204 DASVDIWSLGILCYEFLYGVPPFEAMEHSDTYRRIVQVDLKFPPKPIISASAKDLISQML 263

Query: 254 VKDSSRRLSLEKIVEHPWIIKNADPSGI 282
           VK+SS+RL L K++EHPWI++NADPSGI
Sbjct: 264 VKESSQRLPLHKLLEHPWIVQNADPSGI 291

[Close]

BLAST of MC05g0300.1 vs. ExPASy Swiss-Prot
Match: P97477 (Aurora kinase A OS=Mus musculus OX=10090 GN=Aurka PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 5.5e-97
Identity = 170/274 (62.04%), Postives = 213/274 (77.74%), Query Frame = 0


Query: 10  ETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLRRE 69
           +T  RQW+L+DFD+G+PLGKGKFG VYLARE +SK+I+ALKV+FK Q+EK  + HQLRRE
Sbjct: 113 DTKKRQWTLEDFDIGRPLGKGKFGNVYLARERQSKFILALKVLFKTQLEKANVEHQLRRE 172

Query: 70  MEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSLT 129
           +EIQ+ LRHPNILRLYG+FHDA R++LILEYA  G +YREL+K     E++ ATYI  L 
Sbjct: 173 VEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELA 232

Query: 130 QALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRR-TMCGTLDYLAPEMV 189
            AL+YCH K VIHRDIKPENLLL   G LKIADFGWSV + S RR TMCGTLDYL PEM+
Sbjct: 233 NALSYCHSKRVIHRDIKPENLLLGSNGELKIADFGWSVHAPSSRRTTMCGTLDYLPPEMI 292

Query: 190 ENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDL 249
           E + HD  VD W+LG+LCYEFL G+PPFEA +  +T++RI +V+  FP    V+  ARDL
Sbjct: 293 EGRMHDEKVDLWSLGVLCYEFLVGMPPFEAHTYQETYRRISRVEFTFP--DFVTEGARDL 352

Query: 250 IGRLLVKDSSRRLSLEKIVEHPWIIKNAD--PSG 281
           I RLL  ++S+RL+L +++EHPWI  N+   P+G
Sbjct: 353 ISRLLKHNASQRLTLAEVLEHPWIKANSSKPPTG 384

[Close]

BLAST of MC05g0300.1 vs. ExPASy Swiss-Prot
Match: Q2TA06 (Aurora kinase A OS=Bos taurus OX=9913 GN=AURKA PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.1e-96
Identity = 168/273 (61.54%), Postives = 212/273 (77.66%), Query Frame = 0


Query: 8   DNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLR 67
           + E+  RQW+L+DF++G+PLGKGKFG VYLARE +SK+I+ALKV+FK Q+EK  + HQLR
Sbjct: 120 NEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLR 179

Query: 68  REMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILS 127
           RE+EIQ+ LRHPNILRLYG+FHDA R++LILEYA  G +YREL+K     E++ ATYI  
Sbjct: 180 REVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGAVYRELQKLSKFDEQRTATYITE 239

Query: 128 LTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRR-TMCGTLDYLAPE 187
           L  AL+YCH K VIHRDIKPENLLL   G LKIADFGWSV + S RR T+CGTLDYL PE
Sbjct: 240 LANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPE 299

Query: 188 MVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEAR 247
           M+E + HD  VD W+LG+LCYEFL G PPFEA++  +T++RI +V+  FP    V   AR
Sbjct: 300 MIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEADTYQETYRRISRVEFTFP--DCVPEGAR 359

Query: 248 DLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPS 280
           DLI RLL  + S+R +L++++EHPWII N+ PS
Sbjct: 360 DLISRLLKHNPSQRPTLKEVLEHPWIIANSKPS 390

[Close]

BLAST of MC05g0300.1 vs. NCBI nr
Match: XP_022147052.1 (serine/threonine-protein kinase Aurora-3 [Momordica charantia])

HSP 1 Score: 582 bits (1501), Expect = 6.43e-210
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKY 60
           MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKY
Sbjct: 1   MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKY 60

Query: 61  RIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQ 120
           RIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQ
Sbjct: 61  RIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQ 120

Query: 121 AATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTL 180
           AATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTL
Sbjct: 121 AATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTL 180

Query: 181 DYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQ 240
           DYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQ
Sbjct: 181 DYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQ 240

Query: 241 VSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICNR 284
           VSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICNR
Sbjct: 241 VSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICNR 284

[Close]

BLAST of MC05g0300.1 vs. NCBI nr
Match: XP_022938676.1 (serine/threonine-protein kinase Aurora-3-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 546 bits (1407), Expect = 1.54e-195
Identity = 266/282 (94.33%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           KS  K++N TP RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR
Sbjct: 3   KSLMKEENGTPKRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSE+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKR TMCGTLD
Sbjct: 123 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRHTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVENKAHD+AVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFP TP V
Sbjct: 183 YLAPEMVENKAHDYAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPSTPHV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICN 283
           S EA+DLIGRLLVKDSSRRLSL+KIVEHPWII NADPSGIC+
Sbjct: 243 SSEAKDLIGRLLVKDSSRRLSLQKIVEHPWIIMNADPSGICS 284

[Close]

BLAST of MC05g0300.1 vs. NCBI nr
Match: XP_022974119.1 (serine/threonine-protein kinase Aurora-3 [Cucurbita maxima])

HSP 1 Score: 546 bits (1407), Expect = 1.54e-195
Identity = 266/282 (94.33%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           KS  K++N TP RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR
Sbjct: 3   KSLMKEENGTPKRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSE+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKR TMCGTLD
Sbjct: 123 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRHTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVENKAHD+AVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFP TP V
Sbjct: 183 YLAPEMVENKAHDYAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPSTPHV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICN 283
           S EA+DLIGRLLVKDSSRRLSL+KIVEHPWII NADPSGIC+
Sbjct: 243 SSEAKDLIGRLLVKDSSRRLSLQKIVEHPWIIMNADPSGICS 284

[Close]

BLAST of MC05g0300.1 vs. NCBI nr
Match: KAG7028650.1 (Serine/threonine-protein kinase Aurora-3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 546 bits (1407), Expect = 4.48e-195
Identity = 266/282 (94.33%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           KS  K++N TP RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR
Sbjct: 32  KSLMKEENGTPKRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 91

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSE+QA
Sbjct: 92  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEQQA 151

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKR TMCGTLD
Sbjct: 152 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRHTMCGTLD 211

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVENKAHD+AVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFP TP V
Sbjct: 212 YLAPEMVENKAHDYAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPSTPHV 271

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICN 283
           S EA+DLIGRLLVKDSSRRLSL+KIVEHPWII NADPSGIC+
Sbjct: 272 SSEAKDLIGRLLVKDSSRRLSLQKIVEHPWIIMNADPSGICS 313

[Close]

BLAST of MC05g0300.1 vs. NCBI nr
Match: XP_023540225.1 (serine/threonine-protein kinase Aurora-3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545 bits (1403), Expect = 6.26e-195
Identity = 265/281 (94.31%), Postives = 272/281 (96.80%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           KS  K++N TP RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR
Sbjct: 3   KSLMKEENGTPKRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHL+E+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLNEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKR TMCGTLD
Sbjct: 123 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRHTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVENKAHD+AVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFP TP V
Sbjct: 183 YLAPEMVENKAHDYAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPSTPHV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGIC 282
           S EA+DLIGRLLVKDSSRRLSL+KIVEHPWII NADPSGIC
Sbjct: 243 SSEAKDLIGRLLVKDSSRRLSLQKIVEHPWIIMNADPSGIC 283

[Close]

BLAST of MC05g0300.1 vs. ExPASy TrEMBL
Match: A0A6J1D1A9 (Aurora kinase OS=Momordica charantia OX=3673 GN=LOC111016082 PE=3 SV=1)

HSP 1 Score: 582 bits (1501), Expect = 3.11e-210
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKY 60
           MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKY
Sbjct: 1   MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKY 60

Query: 61  RIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQ 120
           RIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQ
Sbjct: 61  RIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQ 120

Query: 121 AATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTL 180
           AATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTL
Sbjct: 121 AATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTL 180

Query: 181 DYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQ 240
           DYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQ
Sbjct: 181 DYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQ 240

Query: 241 VSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICNR 284
           VSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICNR
Sbjct: 241 VSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICNR 284

[Close]

BLAST of MC05g0300.1 vs. ExPASy TrEMBL
Match: A0A6J1IGM3 (Aurora kinase OS=Cucurbita maxima OX=3661 GN=LOC111472726 PE=3 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 7.45e-196
Identity = 266/282 (94.33%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           KS  K++N TP RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR
Sbjct: 3   KSLMKEENGTPKRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSE+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKR TMCGTLD
Sbjct: 123 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRHTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVENKAHD+AVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFP TP V
Sbjct: 183 YLAPEMVENKAHDYAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPSTPHV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICN 283
           S EA+DLIGRLLVKDSSRRLSL+KIVEHPWII NADPSGIC+
Sbjct: 243 SSEAKDLIGRLLVKDSSRRLSLQKIVEHPWIIMNADPSGICS 284

[Close]

BLAST of MC05g0300.1 vs. ExPASy TrEMBL
Match: A0A6J1FDT7 (Aurora kinase OS=Cucurbita moschata OX=3662 GN=LOC111444816 PE=3 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 7.45e-196
Identity = 266/282 (94.33%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           KS  K++N TP RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR
Sbjct: 3   KSLMKEENGTPKRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSE+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKR TMCGTLD
Sbjct: 123 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRHTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVENKAHD+AVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFP TP V
Sbjct: 183 YLAPEMVENKAHDYAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPSTPHV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICN 283
           S EA+DLIGRLLVKDSSRRLSL+KIVEHPWII NADPSGIC+
Sbjct: 243 SSEAKDLIGRLLVKDSSRRLSLQKIVEHPWIIMNADPSGICS 284

[Close]

BLAST of MC05g0300.1 vs. ExPASy TrEMBL
Match: A0A6J1FEQ5 (ankyrin repeat and zinc finger domain-containing protein 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444816 PE=3 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 6.88e-188
Identity = 266/282 (94.33%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           KS  K++N TP RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR
Sbjct: 3   KSLMKEENGTPKRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSE+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKR TMCGTLD
Sbjct: 123 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRHTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVENKAHD+AVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFP TP V
Sbjct: 183 YLAPEMVENKAHDYAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPSTPHV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICN 283
           S EA+DLIGRLLVKDSSRRLSL+KIVEHPWII NADPSGIC+
Sbjct: 243 SSEAKDLIGRLLVKDSSRRLSLQKIVEHPWIIMNADPSGICS 284

[Close]

BLAST of MC05g0300.1 vs. ExPASy TrEMBL
Match: A0A0A0L3R7 (Aurora kinase OS=Cucumis sativus OX=3659 GN=Csa_3G124980 PE=3 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 1.87e-184
Identity = 248/283 (87.63%), Postives = 268/283 (94.70%), Query Frame = 0


Query: 1   MKSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKY 60
           MK  AK++NE+P RQWSL+DFDVGKPLGKGKFGRVYLAREV+SKYIVALKVIF+EQM+KY
Sbjct: 1   MKYLAKNNNESPKRQWSLKDFDVGKPLGKGKFGRVYLAREVRSKYIVALKVIFREQMKKY 60

Query: 61  RIHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQ 120
            IH QL REMEIQTSLRHPNILRLYGWFHDAERIF+ILEYAHRGELYRELRK+GHLSEKQ
Sbjct: 61  GIHRQLMREMEIQTSLRHPNILRLYGWFHDAERIFMILEYAHRGELYRELRKNGHLSEKQ 120

Query: 121 AATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTL 180
           AATY+LSLTQALAYCHEK VIHRDIKPENLLLDHEGRLKI DFGW+VQSRSKR TMCGTL
Sbjct: 121 AATYMLSLTQALAYCHEKDVIHRDIKPENLLLDHEGRLKIGDFGWAVQSRSKRYTMCGTL 180

Query: 181 DYLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQ 240
           DYLAPEMVENK HD A+DNWT+GILCYEFLYGVPPFEAESQSDTF+RI KV+LNFP TP+
Sbjct: 181 DYLAPEMVENKGHDFAIDNWTMGILCYEFLYGVPPFEAESQSDTFQRIRKVELNFPSTPR 240

Query: 241 VSPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGICN 283
           +S EARDLIGRLLVKDSS+RLSL++I EHPWIIKNADPSGICN
Sbjct: 241 ISTEARDLIGRLLVKDSSKRLSLQEIEEHPWIIKNADPSGICN 283

[Close]

BLAST of MC05g0300.1 vs. TAIR 10
Match: AT2G45490.1 (ataurora3 )

HSP 1 Score: 479.2 bits (1232), Expect = 2.3e-135
Identity = 224/281 (79.72%), Postives = 254/281 (90.39%), Query Frame = 0


Query: 2   KSQAKDDNETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYR 61
           K   + D     +QWSL DF++G+PLGKGKFGRVYLARE KSKYIVALKVIFKEQ+EKY+
Sbjct: 3   KKSTESDAGNTEKQWSLADFEIGRPLGKGKFGRVYLAREAKSKYIVALKVIFKEQIEKYK 62

Query: 62  IHHQLRREMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQA 121
           IHHQLRREMEIQTSLRHPNILRL+GWFHD ERIFLILEYAH GELY  L+++GHL+E+QA
Sbjct: 63  IHHQLRREMEIQTSLRHPNILRLFGWFHDNERIFLILEYAHGGELYGVLKQNGHLTEQQA 122

Query: 122 ATYILSLTQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLD 181
           ATYI SL+QALAYCH K VIHRDIKPENLLLDHEGRLKIADFGWSVQS +KR+TMCGTLD
Sbjct: 123 ATYIASLSQALAYCHGKCVIHRDIKPENLLLDHEGRLKIADFGWSVQSSNKRKTMCGTLD 182

Query: 182 YLAPEMVENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQV 241
           YLAPEMVEN+ HD+AVDNWTLGILCYEFLYG PPFEAESQ DTFKRI+K+DL+FPLTP V
Sbjct: 183 YLAPEMVENRDHDYAVDNWTLGILCYEFLYGNPPFEAESQKDTFKRILKIDLSFPLTPNV 242

Query: 242 SPEARDLIGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGIC 283
           S EA++LI +LLVKD S+RLS+EKI++HPWI+KNADP G+C
Sbjct: 243 SEEAKNLISQLLVKDPSKRLSIEKIMQHPWIVKNADPKGVC 283

[Close]

BLAST of MC05g0300.1 vs. TAIR 10
Match: AT2G25880.1 (ataurora2 )

HSP 1 Score: 394.0 bits (1011), Expect = 9.9e-110
Identity = 179/273 (65.57%), Postives = 231/273 (84.62%), Query Frame = 0


Query: 9   NETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLRR 68
           +E   ++W+  DFD+GKPLG+GKFG VYLARE +S +IVALKV+FK Q+++ ++ HQLRR
Sbjct: 13  SEAAQKRWTTSDFDIGKPLGRGKFGHVYLAREKRSDHIVALKVLFKAQLQQSQVEHQLRR 72

Query: 69  EMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSL 128
           E+EIQ+ LRHPNILRLYG+F+D +R++LILEYA RGELY+EL+K  + SE++AATY+ SL
Sbjct: 73  EVEIQSHLRHPNILRLYGYFYDQKRVYLILEYAVRGELYKELQKCKYFSERRAATYVASL 132

Query: 129 TQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLDYLAPEMV 188
            +AL YCH KHVIHRDIKPENLL+  +G LKIADFGWSV + ++RRTMCGTLDYL PEMV
Sbjct: 133 ARALIYCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMV 192

Query: 189 ENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDL 248
           E+  HD +VD W+LGILCYEFLYGVPPFEA   S+T+KRI++VDL FP  P VS  A+DL
Sbjct: 193 ESVEHDASVDIWSLGILCYEFLYGVPPFEAREHSETYKRIVQVDLKFPPKPIVSSSAKDL 252

Query: 249 IGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGI 282
           I ++LVK+S++RL+L K++EHPWI++NADPSG+
Sbjct: 253 ISQMLVKESTQRLALHKLLEHPWIVQNADPSGL 285

[Close]

BLAST of MC05g0300.1 vs. TAIR 10
Match: AT4G32830.1 (ataurora1 )

HSP 1 Score: 393.7 bits (1010), Expect = 1.3e-109
Identity = 178/268 (66.42%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 14  RQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLRREMEIQ 73
           ++W+L DFD+GKPLG+GKFG VYLARE +S ++VALKV+FK Q+++ ++ HQLRRE+EIQ
Sbjct: 24  KRWTLSDFDIGKPLGRGKFGHVYLAREKRSNHVVALKVLFKSQLQQSQVEHQLRREVEIQ 83

Query: 74  TSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSLTQALA 133
           + LRHPNILRLYG+F+D +R++LILEYA RGELY++L+K  + SE++AATY+ SL +AL 
Sbjct: 84  SHLRHPNILRLYGYFYDQKRVYLILEYAARGELYKDLQKCKYFSERRAATYVASLARALI 143

Query: 134 YCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLDYLAPEMVENKAH 193
           YCH KHVIHRDIKPENLL+  +G LKIADFGWSV + ++RRTMCGTLDYL PEMVE+  H
Sbjct: 144 YCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMVESVEH 203

Query: 194 DHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDLIGRLL 253
           D +VD W+LGILCYEFLYGVPPFEA   SDT++RI++VDL FP  P +S  A+DLI ++L
Sbjct: 204 DASVDIWSLGILCYEFLYGVPPFEAMEHSDTYRRIVQVDLKFPPKPIISASAKDLISQML 263

Query: 254 VKDSSRRLSLEKIVEHPWIIKNADPSGI 282
           VK+SS+RL L K++EHPWI++NADPSGI
Sbjct: 264 VKESSQRLPLHKLLEHPWIVQNADPSGI 291

[Close]

BLAST of MC05g0300.1 vs. TAIR 10
Match: AT2G25880.2 (ataurora2 )

HSP 1 Score: 330.5 bits (846), Expect = 1.3e-90
Identity = 157/273 (57.51%), Postives = 205/273 (75.09%), Query Frame = 0


Query: 9   NETPMRQWSLQDFDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQMEKYRIHHQLRR 68
           +E   ++W+  DFD+GKPLG+GKFG VYLARE +S +IVALKV+FK Q+++ ++ HQLRR
Sbjct: 13  SEAAQKRWTTSDFDIGKPLGRGKFGHVYLAREKRSDHIVALKVLFKAQLQQSQVEHQLRR 72

Query: 69  EMEIQTSLRHPNILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSL 128
           E+EIQ+ LRHPNILRLYG+F+D +R++LILEYA RGELY+EL+K  + SE++AAT     
Sbjct: 73  EVEIQSHLRHPNILRLYGYFYDQKRVYLILEYAVRGELYKELQKCKYFSERRAAT----- 132

Query: 129 TQALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSRSKRRTMCGTLDYLAPEMV 188
                                      G LKIADFGWSV + ++RRTMCGTLDYL PEMV
Sbjct: 133 ---------------------------GELKIADFGWSVHTFNRRRTMCGTLDYLPPEMV 192

Query: 189 ENKAHDHAVDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDL 248
           E+  HD +VD W+LGILCYEFLYGVPPFEA   S+T+KRI++VDL FP  P VS  A+DL
Sbjct: 193 ESVEHDASVDIWSLGILCYEFLYGVPPFEAREHSETYKRIVQVDLKFPPKPIVSSSAKDL 252

Query: 249 IGRLLVKDSSRRLSLEKIVEHPWIIKNADPSGI 282
           I ++LVK+S++RL+L K++EHPWI++NADPSG+
Sbjct: 253 ISQMLVKESTQRLALHKLLEHPWIVQNADPSGL 253

[Close]

BLAST of MC05g0300.1 vs. TAIR 10
Match: AT5G10930.1 (CBL-interacting protein kinase 5 )

HSP 1 Score: 199.5 bits (506), Expect = 3.6e-51
Identity = 109/265 (41.13%), Postives = 158/265 (59.62%), Query Frame = 0


Query: 21  FDVGKPLGKGKFGRVYLAREVKSKYIVALKVIFKEQ-MEKYRIHHQLRREMEIQTSLRHP 80
           +++G+ LGKG F +VY  +E+     VA+KVI K+Q M++  +  Q++RE+ I   +RHP
Sbjct: 12  YEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISIMKLVRHP 71

Query: 81  NILRLYGWFHDAERIFLILEYAHRGELYRELRKSGHLSEKQAATYILSLTQALAYCHEKH 140
           NI+ L        +IF ++E+   GEL+ ++ K G L E  A  Y   L  A+ YCH + 
Sbjct: 72  NIVELKEVMATKTKIFFVMEFVKGGELFCKISK-GKLHEDAARRYFQQLISAVDYCHSRG 131

Query: 141 VIHRDIKPENLLLDHEGRLKIADFGWS-----VQSRSKRRTMCGTLDYLAPEMVENKAHD 200
           V HRD+KPENLLLD  G LKI+DFG S     +       T CGT  Y+APE+++ K +D
Sbjct: 132 VSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEVLKKKGYD 191

Query: 201 HA-VDNWTLGILCYEFLYGVPPFEAESQSDTFKRIMKVDLNFPLTPQVSPEARDLIGRLL 260
            A  D W+ G++ Y  L G  PF+ E+  + +++I + D  FP  P  SPEAR LI +LL
Sbjct: 192 GAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFP--PWFSPEARRLISKLL 251

Query: 261 VKDSSRRLSLEKIVEHPWIIKNADP 279
           V D  RR+S+  I+  PW+ KN  P
Sbjct: 252 VVDPDRRISIPAIMRTPWLRKNFTP 273

The following BLAST results are available for this feature:

BLAST of MC05g0300.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64629	3.3e-134	79.72	Serine/threonine-protein kinase Aurora-3 OS=Arabidopsis thaliana OX=3702 GN=AUR3...	[more]
Q683C9	1.4e-108	65.57	Serine/threonine-protein kinase Aurora-2 OS=Arabidopsis thaliana OX=3702 GN=AUR2...	[more]
Q9M077	1.8e-108	66.42	Serine/threonine-protein kinase Aurora-1 OS=Arabidopsis thaliana OX=3702 GN=AUR1...	[more]
P97477	5.5e-97	62.04	Aurora kinase A OS=Mus musculus OX=10090 GN=Aurka PE=1 SV=1	[more]
Q2TA06	2.1e-96	61.54	Aurora kinase A OS=Bos taurus OX=9913 GN=AURKA PE=2 SV=1	[more]

BLAST of MC05g0300.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147052.1	6.43e-210	100.00	serine/threonine-protein kinase Aurora-3 [Momordica charantia]	[more]
XP_022938676.1	1.54e-195	94.33	serine/threonine-protein kinase Aurora-3-like isoform X3 [Cucurbita moschata]	[more]
XP_022974119.1	1.54e-195	94.33	serine/threonine-protein kinase Aurora-3 [Cucurbita maxima]	[more]
KAG7028650.1	4.48e-195	94.33	Serine/threonine-protein kinase Aurora-3, partial [Cucurbita argyrosperma subsp....	[more]
XP_023540225.1	6.26e-195	94.31	serine/threonine-protein kinase Aurora-3 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC05g0300.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1A9	3.11e-210	100.00	Aurora kinase OS=Momordica charantia OX=3673 GN=LOC111016082 PE=3 SV=1	[more]
A0A6J1IGM3	7.45e-196	94.33	Aurora kinase OS=Cucurbita maxima OX=3661 GN=LOC111472726 PE=3 SV=1	[more]
A0A6J1FDT7	7.45e-196	94.33	Aurora kinase OS=Cucurbita moschata OX=3662 GN=LOC111444816 PE=3 SV=1	[more]
A0A6J1FEQ5	6.88e-188	94.33	ankyrin repeat and zinc finger domain-containing protein 1-like isoform X1 OS=Cu...	[more]
A0A0A0L3R7	1.87e-184	87.63	Aurora kinase OS=Cucumis sativus OX=3659 GN=Csa_3G124980 PE=3 SV=1	[more]

BLAST of MC05g0300.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45490.1	2.3e-135	79.72	ataurora3	[more]
AT2G25880.1	9.9e-110	65.57	ataurora2	[more]
AT4G32830.1	1.3e-109	66.42	ataurora1	[more]
AT2G25880.2	1.3e-90	57.51	ataurora2	[more]
AT5G10930.1	3.6e-51	41.13	CBL-interacting protein kinase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 21..272 e-value: 5.4E-97 score: 338.2
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 22..272 e-value: 1.5E-75 score: 254.0
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 21..272 score: 51.155907
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 105..280 e-value: 1.8E-58 score: 199.8
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 9..104 e-value: 5.0E-34 score: 118.1
None	No IPR available	PIRSF	PIRSF000654	ILK	coord: 1..276 e-value: 2.1E-40 score: 136.7
None	No IPR available	PIRSR	PIRSR037568-2	PIRSR037568-2	coord: 17..272 e-value: 2.9E-40 score: 135.4
None	No IPR available	PIRSR	PIRSR000556-2	PIRSR000556-2	coord: 6..218 e-value: 7.6E-22 score: 75.4
None	No IPR available	CDD	cd14007	STKc_Aurora	coord: 20..272 e-value: 3.83538E-165 score: 456.166
IPR030616	Aurora kinase	PANTHER	PTHR24350	SERINE/THREONINE-PROTEIN KINASE IAL-RELATED	coord: 3..282
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 140..152
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 27..54
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 13..276

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC05g0300	MC05g0300	gene

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g0300.1-utr3	MC05g0300.1-utr3-MC05:2250620..2250873	utr3

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g0300.1-cds	MC05g0300.1-cds-MC05:2250874..2250975	CDS
MC05g0300.1-cds	MC05g0300.1-cds-MC05:2251161..2251233	CDS
MC05g0300.1-cds	MC05g0300.1-cds-MC05:2251876..2252090	CDS
MC05g0300.1-cds	MC05g0300.1-cds-MC05:2252753..2252848	CDS
MC05g0300.1-cds	MC05g0300.1-cds-MC05:2253140..2253382	CDS
MC05g0300.1-cds	MC05g0300.1-cds-MC05:2253909..2254034	CDS

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g0300.1-utr5	MC05g0300.1-utr5-MC05:2254035..2254229	utr5

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC05g0300.1	MC05g0300.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0035404	histone-serine phosphorylation
biological_process	GO:0007052	mitotic spindle organization
biological_process	GO:0032465	regulation of cytokinesis
cellular_component	GO:0032133	chromosome passenger complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005876	spindle microtubule
cellular_component	GO:0051233	spindle midzone
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0035174	histone serine kinase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity