MC05g0270.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0270.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	tRNA (Guanine-N(7)-)-methyltransferase
Location	MC05: 1976387 .. 1979174 (-)
Sequence length	1244
RNA-Seq Expression	MC05g0270.1
Synteny	MC05g0270.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGGAAAAAGGTAAAATTTAAACCACACGAAATTGGAAGAAAGAGGAAAAATAGATAGAAGCAGCGCCATTCTTGAGAGCTCAGCGGGCGCGGCAAAAATGGCGCCGTCCCTTTGCTTCCAGTTACACACTTGCTCGCAGCTCGGTTGCATTGCTGCTGCTAAGGCGGCGGCGCCGTCGAGCGTCGCAACCGCAAATAGAACATTGTCTCCGTTCCTCAGGCTCAGACGACCATTTCTCCATAGCTTCTGTGTAAGATTGGGCCCTTCCGCCGAGGTTTTCGAGCCGCAACATCTCCGGAGTCCGGAACTCGTGGCGTTGGAATATGCCGACCTTAATCTCCCTCACAGGATCTTCGAGGTTTGTTCTCTGTTCACGAATTTTGCCCCTAATTTCTGAAATTGCAATTTCATCTCGAAGTAGAAAGTGGTGATAATTAGAATTGATGTGCATTTGACTTGAATTTGGTAATTGCAGGATTCGGGTCACGTTAGAATCAGACAGCACGTTAACCCTCTCAGCTCTTCTTTCGCTGTATGTAATTTGAACTTTGGAACTATGGCTTTGAAATTTAGTTGAGTGTTGGTGGCTTAAAGGAAGGTTCCCCATCTTTTACCTTTCAGGTCCCTGCACCAGCACCTAATTGGAACGAAGTCTTCAAGGATCCCACATTACCCCTCATGGTCGATATAGGAAGTGGTGAGCGAGTTTTCTCAGTTCATATTATACAGACCTCTGACATACCTGTTTTCTCCATAATTGCCTTATTTTTTAGCATCCGAGCTAGTTTATATGTACTTTAACAAATTTCACGAGATAACTGTCCATCTTATATAACATTTGGCTGAGCCCATATTCCTAAAATCCTTCTACCTTTTGCCTTTGATTACTAAGTCAACCCAGGATGATTACTATTGTCATTGTCTTTTTATCTTTTAGCCTCCTGCTTTGAATGGAAGACAGTCATGAACAAATCTTCAAAGCTGCTGCTGCTGCTTTGAATAAGACGTACAAACTGAGTAAATAAAAGGGTAATCTGGCTGGTGAAAAAATTGTATTTAACAACGGTTAATGTCTGAAGAATGAAATATCATTCGCAAGTAACATATTGATTAGTGCATTCACTAATCTACTTTACCTTGCACTGTAACTCAAAATTAAACTTGACATGCATAATAAAGCAGCTATAGTTATGAAATCAATACAGTACATGCGGAGCAAAAAACTCTGAGCAATAGCAGCTATGAGCTACCCCTCATGACTTCTATATGTTGGTGCTAGCCAGTAAATGCTAATAAAAATGTCAGTCCCCTAAGTTGACCCGAAGGATTTACAGTGTTCGTAGTGATAGTAAGGTTTCAAATGCTCGTTGCATATCAAAAAGTATGTATTCACATGCAAGAAAATTATCTGTCTGATATGCTGTAATGATCTACGATAGGTCATTACTTTTAAAAATGTTCAAAACCTTGATTACCTCCCAACATTGGTTTAATTAGCCTCATTACATGCTTCTTGTGGGATTTATGAGGCTGTTTTATGCTTTTTTGTCCATTAAGAATCTAAACATGTATTATTACTCCCAGGTAGTGGCAGATTTCTCCTTTGGCTTGCAAAAAGACATCTTGATATGAGAAATTTCTTGGGTCTGGAAATACGACATAAAGTATGCAACCAAAAATTTTCACTTTTGACGCACCCTTAGTTGGTTTGAACTATCTGATTTTCATTCTGTCTAGGCCTTATATTGTGGTTTGGTTCCTTTTGTGCTCTTGCAGCTGGTCAAGCGTGCCGATATCTGGGTCAAAGAGTTGGGACTTAGCAACGTGTAAGGACCTTAACTTCATATCAGGTAGTGAAAAACAACCTCCTTTATACTACTGGTGCATTAGTAATGAACTTTTTCAAAACTTGAATGCAGACATTTCATGTTTGCAAATGCTACAATTTCCTTTAAACAGATCATATCTTCCTATCCTGGACCCCTGATGCTGGTTTCCATACTGGTTAGTGTAACACACTAGATCAACATATTTTTTTCGGTTACCGTTGATCTGATCCGATAATTATCGACAAAGAAAGAACTCGCTCACGTTTAATCTCAGTGCCCAGATCCCCATTTCAAGAAACGGCATCACAAGCGAAGGGTTGTGCAGAAGCCTTTAGTAGAATCTATAGTAGACAGTTTAACTCCTGGAGGGCAGGTATATGATCTTGTAATTCATCCATTACATTGAAAGTTTGGCTCTCTGTTCTTTGAACTTGATGCAATCTGATGCTGTTTTCTTTTGAGATCAATGCAACTTGATGAGTCTGATGTTGGAACTTCTCTTCTTTATGAGATTCTACAGGTGCTCATACAATCTGATGTTCTTGAAGTGGCAGTCGACATGAGGCTTTGGTTCGATTCTGAAGCCGATGTTCTAAAACACATCGATGTGCTCGACCCGAGTGTTTTGTGTGACGAAGATGGATGGTTGTTGAACAATCCAATGGGAATAAGGACCGAAAGAGAAATTCATGCCGAATCCGAAGGTGCGAAAATTTATAGAAGAATGTATCAAAAGTTTACATGACAAAAGAATAACTGAAGAGTAACTAGATAGATTGTTTAATGGTAATCTGTTTGCATTTTAGTCAGTATTTACTGGTTTATCTTTTGAGGCCATCTATCTACTCTTATTGTTAGGAAATTATGAAATATTTTCTTCAAATAAATTTATTACATATTTATGAAAATAATAAAATAGAAATCTTTTATAATATTTCAATTAAAATATAGTTTGGTC

mRNA sequence

GGGGAAAAAGGTAAAATTTAAACCACACGAAATTGGAAGAAAGAGGAAAAATAGATAGAAGCAGCGCCATTCTTGAGAGCTCAGCGGGCGCGGCAAAAATGGCGCCGTCCCTTTGCTTCCAGTTACACACTTGCTCGCAGCTCGGTTGCATTGCTGCTGCTAAGGCGGCGGCGCCGTCGAGCGTCGCAACCGCAAATAGAACATTGTCTCCGTTCCTCAGGCTCAGACGACCATTTCTCCATAGCTTCTGTGTAAGATTGGGCCCTTCCGCCGAGGTTTTCGAGCCGCAACATCTCCGGAGTCCGGAACTCGTGGCGTTGGAATATGCCGACCTTAATCTCCCTCACAGGATCTTCGAGGATTCGGGTCACGTTAGAATCAGACAGCACGTTAACCCTCTCAGCTCTTCTTTCGCTGTCCCTGCACCAGCACCTAATTGGAACGAAGTCTTCAAGGATCCCACATTACCCCTCATGGTCGATATAGGAAGTGGTAGTGGCAGATTTCTCCTTTGGCTTGCAAAAAGACATCTTGATATGAGAAATTTCTTGGGTCTGGAAATACGACATAAACTGGTCAAGCGTGCCGATATCTGGGTCAAAGAGTTGGGACTTAGCAACGTACATTTCATGTTTGCAAATGCTACAATTTCCTTTAAACAGATCATATCTTCCTATCCTGGACCCCTGATGCTGGTTTCCATACTGTGCCCAGATCCCCATTTCAAGAAACGGCATCACAAGCGAAGGGTTGTGCAGAAGCCTTTAGTAGAATCTATAGTAGACAGTTTAACTCCTGGAGGGCAGGTGCTCATACAATCTGATGTTCTTGAAGTGGCAGTCGACATGAGGCTTTGGTTCGATTCTGAAGCCGATGTTCTAAAACACATCGATGTGCTCGACCCGAGTGTTTTGTGTGACGAAGATGGATGGTTGTTGAACAATCCAATGGGAATAAGGACCGAAAGAGAAATTCATGCCGAATCCGAAGGTGCGAAAATTTATAGAAGAATGTATCAAAAGTTTACATGACAAAAGAATAACTGAAGAGTAACTAGATAGATTGTTTAATGGTAATCTGTTTGCATTTTAGTCAGTATTTACTGGTTTATCTTTTGAGGCCATCTATCTACTCTTATTGTTAGGAAATTATGAAATATTTTCTTCAAATAAATTTATTACATATTTATGAAAATAATAAAATAGAAATCTTTTATAATATTTCAATTAAAATATAGTTTGGTC

Coding sequence (CDS)

ATGGCGCCGTCCCTTTGCTTCCAGTTACACACTTGCTCGCAGCTCGGTTGCATTGCTGCTGCTAAGGCGGCGGCGCCGTCGAGCGTCGCAACCGCAAATAGAACATTGTCTCCGTTCCTCAGGCTCAGACGACCATTTCTCCATAGCTTCTGTGTAAGATTGGGCCCTTCCGCCGAGGTTTTCGAGCCGCAACATCTCCGGAGTCCGGAACTCGTGGCGTTGGAATATGCCGACCTTAATCTCCCTCACAGGATCTTCGAGGATTCGGGTCACGTTAGAATCAGACAGCACGTTAACCCTCTCAGCTCTTCTTTCGCTGTCCCTGCACCAGCACCTAATTGGAACGAAGTCTTCAAGGATCCCACATTACCCCTCATGGTCGATATAGGAAGTGGTAGTGGCAGATTTCTCCTTTGGCTTGCAAAAAGACATCTTGATATGAGAAATTTCTTGGGTCTGGAAATACGACATAAACTGGTCAAGCGTGCCGATATCTGGGTCAAAGAGTTGGGACTTAGCAACGTACATTTCATGTTTGCAAATGCTACAATTTCCTTTAAACAGATCATATCTTCCTATCCTGGACCCCTGATGCTGGTTTCCATACTGTGCCCAGATCCCCATTTCAAGAAACGGCATCACAAGCGAAGGGTTGTGCAGAAGCCTTTAGTAGAATCTATAGTAGACAGTTTAACTCCTGGAGGGCAGGTGCTCATACAATCTGATGTTCTTGAAGTGGCAGTCGACATGAGGCTTTGGTTCGATTCTGAAGCCGATGTTCTAAAACACATCGATGTGCTCGACCCGAGTGTTTTGTGTGACGAAGATGGATGGTTGTTGAACAATCCAATGGGAATAAGGACCGAAAGAGAAATTCATGCCGAATCCGAAGGTGCGAAAATTTATAGAAGAATGTATCAAAAGTTTACATGA

Protein sequence

MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAKIYRRMYQKFT

Homology

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BLAST of MC05g0270.1 vs. ExPASy Swiss-Prot
Match: Q2JJQ0 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=trmB PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.8e-45
Identity = 104/214 (48.60%), Postives = 131/214 (61.21%), Query Frame = 0


Query: 90  GHVRIRQHVNPLSSSFAVPAPAPNWNEVFKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRN 149
           GH R+RQHVNPL + +  PAP P+W  V++    P  +DIG+GSGRFLL +A+   D  N
Sbjct: 35  GH-RVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRFLLRIAQEQPDW-N 94

Query: 150 FLGLEIRHKLVKRADIWVKELGLSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHF 209
           FLG+EIR  LV+RA+ W  ELGL NVHF+FAN  +S + + +  PG L  V+IL PDP F
Sbjct: 95  FLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTILFPDPWF 154

Query: 210 KKRHHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLEVAVDMRLWFDSEADVLKHIDVLDP 269
           KKRHHKRR+VQ  LV  +   L PG  V +QSD+ EVA +M   F      L+H    DP
Sbjct: 155 KKRHHKRRIVQPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARF------LEHPQFWDP 214

Query: 270 SVLCDEDGWLLNNPMGIRTEREIHAESEGAKIYR 304
                  G L +NP+GI TERE         IYR
Sbjct: 215 -----HQGPLDSNPLGIPTEREWQCLQLDLPIYR 233

[Close]

BLAST of MC05g0270.1 vs. ExPASy Swiss-Prot
Match: Q2JRH1 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=trmB PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.0e-43
Identity = 102/211 (48.34%), Postives = 128/211 (60.66%), Query Frame = 0


Query: 93  RIRQHVNPLSSSFAVPAPAPNWNEVFKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLG 152
           R+RQHVNPL + +  P P P+W  V++    PL +DIGSGSGRFLL +A+   D  NFLG
Sbjct: 34  RLRQHVNPLQAQYQQPTPPPHWERVYRRLGQPLHLDIGSGSGRFLLRMAQEQPDW-NFLG 93

Query: 153 LEIRHKLVKRADIWVKELGLSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKR 212
           +EIR  LV+RA+ W  ELGL NVHF+FAN  +S + + +  PG L  V+I  PDP FKKR
Sbjct: 94  VEIRQPLVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTIQFPDPWFKKR 153

Query: 213 HHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVL 272
           HHKRR+VQ  LV  +   L PG  V +QSDV EVA +M   F      L+H    +P   
Sbjct: 154 HHKRRIVQPRLVADLALLLQPGSPVFLQSDVREVAEEMVDRF------LEHPQFWNP--- 213

Query: 273 CDEDGWLLNNPMGIRTEREIHAESEGAKIYR 304
               G + +NP GI TERE      G  IYR
Sbjct: 214 --YQGPIDDNPFGIPTEREWQCLQLGLPIYR 230

[Close]

BLAST of MC05g0270.1 vs. ExPASy Swiss-Prot
Match: P73161 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=trmB PE=3 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-41
Identity = 96/217 (44.24%), Postives = 127/217 (58.53%), Query Frame = 0


Query: 92  VRIRQHVNPLSSSFAVPAPAPNWNEVFKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFL 151
           VRIRQHVNPLS  +      P+W  V+ D   PL +DIG   GRF L +A++H D  NFL
Sbjct: 4   VRIRQHVNPLSEKYRQVLACPDWATVYDDVQRPLHLDIGCARGRFPLKMAQQHPDW-NFL 63

Query: 152 GLEIRHKLVKRADIWVKELGLSNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKK 211
           G+EIR  LV  A+     LGL N+H++F N  +  ++  S++P  L  VSI  PDP FK+
Sbjct: 64  GVEIRQPLVLEANETGDRLGLKNLHYLFGNINVEPEKFFSAFPPTLQRVSIQFPDPWFKQ 123

Query: 212 RHHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSV 271
           RH+KRRV Q  LV +I ++L PGG+VL+QSDV  VA DMR  F             D  V
Sbjct: 124 RHNKRRVAQPELVTAIANALPPGGEVLLQSDVEPVAQDMRDRFAEN----------DNFV 183

Query: 272 LCDEDGWLLNNPMGIRTEREIHAESEGAKIYRRMYQK 309
              +  WL  NP+G+ TEREI   +    +YR + Q+
Sbjct: 184 FTHDTPWLAENPLGVPTEREIACFNLQRPVYRCLLQR 209

[Close]

BLAST of MC05g0270.1 vs. ExPASy Swiss-Prot
Match: P72546 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=trmB PE=3 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 3.7e-38
Identity = 92/216 (42.59%), Postives = 128/216 (59.26%), Query Frame = 0


Query: 92  VRIRQHVNPLSSSFAVPAPAPNWNEVFKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFL 151
           VR+RQHVNPLS  F V    P+W +V+ D   PL +DIG   GRFLL +A    +  N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQPEW-NYL 63

Query: 152 GLEIRHKLVKRADIWVKELGLSNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 211
           GLEIR  LV  A+   +E  L+N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 212 KRHHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPS 271
           KRH KRRVVQ  LV+++  +L  G +V +QSDVLEV  +M   F +E    +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 272 VLCDEDGWLLNNPMGIRTEREIHAESEGAKIYRRMY 307
                  WL  NP+ + TEREI  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

[Close]

BLAST of MC05g0270.1 vs. ExPASy Swiss-Prot
Match: Q5N2X5 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=trmB PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.7e-38
Identity = 92/216 (42.59%), Postives = 128/216 (59.26%), Query Frame = 0


Query: 92  VRIRQHVNPLSSSFAVPAPAPNWNEVFKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFL 151
           VR+RQHVNPLS  F V    P+W +V+ D   PL +DIG   GRFLL +A    +  N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQPEW-NYL 63

Query: 152 GLEIRHKLVKRADIWVKELGLSNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 211
           GLEIR  LV  A+   +E  L+N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 212 KRHHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPS 271
           KRH KRRVVQ  LV+++  +L  G +V +QSDVLEV  +M   F +E    +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 272 VLCDEDGWLLNNPMGIRTEREIHAESEGAKIYRRMY 307
                  WL  NP+ + TEREI  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

[Close]

BLAST of MC05g0270.1 vs. NCBI nr
Match: XP_022146903.1 (uncharacterized protein LOC111015991 [Momordica charantia])

HSP 1 Score: 632 bits (1630), Expect = 1.05e-228
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV 60
           MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV
Sbjct: 1   MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV 60

Query: 61  FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD 120
           FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD
Sbjct: 61  FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD 120

Query: 121 PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA 180
           PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA
Sbjct: 121 PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA 180

Query: 181 NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ 240
           NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ
Sbjct: 181 NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ 240

Query: 241 SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK 300
           SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK
Sbjct: 241 SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK 300

Query: 301 IYRRMYQKFT 310
           IYRRMYQKFT
Sbjct: 301 IYRRMYQKFT 310

[Close]

BLAST of MC05g0270.1 vs. NCBI nr
Match: XP_022980361.1 (uncharacterized protein LOC111479752 [Cucurbita maxima])

HSP 1 Score: 543 bits (1398), Expect = 2.68e-193
Identity = 269/313 (85.94%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAA---PSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60
           MA SLCFQLHTCSQL CIAAAKAAA   PSSVATANRTLS  L ++RPFLHSFC+  G S
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLS--LLVKRPFLHSFCLPSGSS 60

Query: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQV 240

Query: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300
            IQSDVLEVAVDMRLWFDS  +VLKH+DVLD S+LCD+DGWLLNNPMGIRTEREIHAESE
Sbjct: 241 FIQSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESE 300

Query: 301 GAKIYRRMYQKFT 310
           GA IYRRMYQK+ 
Sbjct: 301 GANIYRRMYQKYV 311

[Close]

BLAST of MC05g0270.1 vs. NCBI nr
Match: XP_023527130.1 (uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540 bits (1392), Expect = 2.20e-192
Identity = 269/313 (85.94%), Postives = 285/313 (91.05%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAA---PSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60
           MA SLCFQLHTCSQL CIAAAKAAA   PSSVATANRTLS  L L+RPFL SFC+  G S
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAVAAPSSVATANRTLS--LLLKRPFLQSFCLPSGSS 60

Query: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQV 240

Query: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300
            IQSDVLEVAVDMRLWFDS  DVLKH+DVLD S+LCD+DGWLL NPMGIRTEREIHAESE
Sbjct: 241 FIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKDGWLLKNPMGIRTEREIHAESE 300

Query: 301 GAKIYRRMYQKFT 310
           GA IYRRMYQK+ 
Sbjct: 301 GANIYRRMYQKYV 311

[Close]

BLAST of MC05g0270.1 vs. NCBI nr
Match: KAG7018929.1 (trmB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 537 bits (1384), Expect = 3.64e-191
Identity = 266/313 (84.98%), Postives = 285/313 (91.05%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAA---PSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60
           MA SLCFQLHTCSQL CIAAAKAAA   PSSVATANRTLS  L ++RPFL SFC+  G S
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLS--LLVKRPFLQSFCLPSGSS 60

Query: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQV 240

Query: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300
            IQSDVLEVAVDMRLWFDS  DVLKH+DVLD S+LCD++GWLL NPMGIRTERE+HAESE
Sbjct: 241 FIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESE 300

Query: 301 GAKIYRRMYQKFT 310
           GA IYRRMYQK+ 
Sbjct: 301 GANIYRRMYQKYV 311

[Close]

BLAST of MC05g0270.1 vs. NCBI nr
Match: XP_022924717.1 (uncharacterized protein LOC111432133 [Cucurbita moschata])

HSP 1 Score: 535 bits (1377), Expect = 4.24e-190
Identity = 266/313 (84.98%), Postives = 284/313 (90.73%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAA---PSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60
           MA SLCFQLHTCSQL CIAAAKAAA   PSSVATANRTLS  L ++RPFL SFC+  G S
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLS--LLVKRPFLQSFCLPSGSS 60

Query: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQV 240

Query: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300
            IQSDVLEVAVDMRLWFDS  +VLKH+DV D S+LCD+DGWLL NPMGIRTEREIHAESE
Sbjct: 241 FIQSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESE 300

Query: 301 GAKIYRRMYQKFT 310
           GA IYRRMYQK+ 
Sbjct: 301 GANIYRRMYQKYV 311

[Close]

BLAST of MC05g0270.1 vs. ExPASy TrEMBL
Match: A0A6J1CZT2 (uncharacterized protein LOC111015991 OS=Momordica charantia OX=3673 GN=LOC111015991 PE=4 SV=1)

HSP 1 Score: 632 bits (1630), Expect = 5.06e-229
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV 60
           MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV
Sbjct: 1   MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV 60

Query: 61  FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD 120
           FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD
Sbjct: 61  FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD 120

Query: 121 PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA 180
           PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA
Sbjct: 121 PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA 180

Query: 181 NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ 240
           NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ
Sbjct: 181 NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ 240

Query: 241 SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK 300
           SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK
Sbjct: 241 SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK 300

Query: 301 IYRRMYQKFT 310
           IYRRMYQKFT
Sbjct: 301 IYRRMYQKFT 310

[Close]

BLAST of MC05g0270.1 vs. ExPASy TrEMBL
Match: A0A6J1ITE1 (uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752 PE=4 SV=1)

HSP 1 Score: 543 bits (1398), Expect = 1.30e-193
Identity = 269/313 (85.94%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAA---PSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60
           MA SLCFQLHTCSQL CIAAAKAAA   PSSVATANRTLS  L ++RPFLHSFC+  G S
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLS--LLVKRPFLHSFCLPSGSS 60

Query: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQV 240

Query: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300
            IQSDVLEVAVDMRLWFDS  +VLKH+DVLD S+LCD+DGWLLNNPMGIRTEREIHAESE
Sbjct: 241 FIQSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESE 300

Query: 301 GAKIYRRMYQKFT 310
           GA IYRRMYQK+ 
Sbjct: 301 GANIYRRMYQKYV 311

[Close]

BLAST of MC05g0270.1 vs. ExPASy TrEMBL
Match: A0A6J1E9S1 (uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC111432133 PE=4 SV=1)

HSP 1 Score: 535 bits (1377), Expect = 2.05e-190
Identity = 266/313 (84.98%), Postives = 284/313 (90.73%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAA---PSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60
           MA SLCFQLHTCSQL CIAAAKAAA   PSSVATANRTLS  L ++RPFL SFC+  G S
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLS--LLVKRPFLQSFCLPSGSS 60

Query: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQV 240

Query: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300
            IQSDVLEVAVDMRLWFDS  +VLKH+DV D S+LCD+DGWLL NPMGIRTEREIHAESE
Sbjct: 241 FIQSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESE 300

Query: 301 GAKIYRRMYQKFT 310
           GA IYRRMYQK+ 
Sbjct: 301 GANIYRRMYQKYV 311

[Close]

BLAST of MC05g0270.1 vs. ExPASy TrEMBL
Match: A0A5A7U1F8 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003710 PE=4 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 1.05e-176
Identity = 248/308 (80.52%), Postives = 267/308 (86.69%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV 60
           MA SLC  L TCS L C+ AAK AAPS VATAN+ LS     +RPFL +F +  G SA++
Sbjct: 1   MASSLCLHLQTCSHLRCLGAAKPAAPSVVATANKILS-----KRPFLRNFSLPSGSSADL 60

Query: 61  FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD 120
            E Q LRS ELVALEYADLNL H++ ED GHVRIRQHVNPLSSSF+VP PAPNW+EVF+D
Sbjct: 61  CERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRD 120

Query: 121 PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA 180
           PTLPLMVDIGSGSGRFLLWLAKRHL+ RNFLGLEIR KLVKRA  WVKELG SNVHFMFA
Sbjct: 121 PTLPLMVDIGSGSGRFLLWLAKRHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMFA 180

Query: 181 NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ 240
           NATISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPLVESIVDSL PGGQV IQ
Sbjct: 181 NATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQ 240

Query: 241 SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK 300
           SDVLEVA DMRLWFDSE D+L+HID +D SVLCD+DGWLLNNPMGIRTEREIHAESEGA 
Sbjct: 241 SDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGAN 300

Query: 301 IYRRMYQK 308
           IYRRMY K
Sbjct: 301 IYRRMYSK 303

[Close]

BLAST of MC05g0270.1 vs. ExPASy TrEMBL
Match: A0A1S3AVT2 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 PE=4 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 2.12e-176
Identity = 248/308 (80.52%), Postives = 267/308 (86.69%), Query Frame = 0


Query: 1   MAPSLCFQLHTCSQLGCIAAAKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPSAEV 60
           MA SLC  L TCS L C+AAAK AAPS VATAN+ LS     +RPFL +F +  G SA++
Sbjct: 1   MASSLCLHLQTCSHLRCLAAAKPAAPSVVATANKILS-----KRPFLRNFSLPSGSSADL 60

Query: 61  FEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKD 120
            E Q LRS ELVALEYADLNL H++ ED GHVRIRQHVNPLSSSF+VP PAPNW+EVF+D
Sbjct: 61  CERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFRD 120

Query: 121 PTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFA 180
           PTLPLMVDIGSGSGRFLLWLAK HL+ RNFLGLEIR KLVKRA  WVKELG SNVHFMFA
Sbjct: 121 PTLPLMVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMFA 180

Query: 181 NATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQ 240
           NATISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRV+QKPLVESIVDSL PGGQV IQ
Sbjct: 181 NATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFIQ 240

Query: 241 SDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAK 300
           SDVLEVA DMRLWFDSE D+L+HID +D SVLCD+DGWLLNNPMGIRTEREIHAESEGA 
Sbjct: 241 SDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGAN 300

Query: 301 IYRRMYQK 308
           IYRRMY K
Sbjct: 301 IYRRMYSK 303

[Close]

BLAST of MC05g0270.1 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 364.8 bits (935), Expect = 7.0e-101
Identity = 175/243 (72.02%), Postives = 206/243 (84.77%), Query Frame = 0


Query: 66  LRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEVFKDPTLPL 125
           LRS ELV LEYA+LNL H+I E+ GHVRIRQHVNPLSSSF+ PAP P W+EV+KDP+LPL
Sbjct: 68  LRSNELVELEYAELNLNHKISEEVGHVRIRQHVNPLSSSFSKPAPVPVWDEVYKDPSLPL 127

Query: 126 MVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFANATIS 185
           MVDIGSGSGRFLLW A ++++ RN+LGLEIR KLVKRA+ WV ELGLSNVHF+FANA +S
Sbjct: 128 MVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGLSNVHFIFANAMVS 187

Query: 186 FKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLE 245
           F+ +ISSYPGPL +VSILCPDPHFKKRH KRRVVQKPLV SI+ +L PGG++ +QSDVL+
Sbjct: 188 FEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLKPGGKIFVQSDVLD 247

Query: 246 VAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESEGAKIYRRM 305
           VA DMR   D E++VL+H+D +D      EDGWL  NPMGIRTEREIHAE EGA+IYRR+
Sbjct: 248 VAQDMRDQLDEESNVLQHMDTVD-----TEDGWLTENPMGIRTEREIHAEFEGARIYRRL 305

Query: 306 YQK 309
           YQK
Sbjct: 308 YQK 305

[Close]

BLAST of MC05g0270.1 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 59.3 bits (142), Expect = 6.3e-09
Identity = 53/175 (30.29%), Postives = 83/175 (47.43%), Query Frame = 0


Query: 93  RIRQHVNPLSSS-FAVP-APA--------PNWNEVFKDPTLPL-MVDIGSGSGRFLLWLA 152
           R R H NPLS S F +P +PA        P + E        +   DIG G G  L+ LA
Sbjct: 23  RARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFADIGCGFGGLLISLA 82

Query: 153 KRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHFMFANATI----SFKQIISSY-PGP 212
               D    +G+E+R K+ +     +  L  ++    + N ++    S K I + +  G 
Sbjct: 83  TLFPDTL-MIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTNSMKYIPNYFEKGQ 142

Query: 213 LMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQVLIQSDVLEVAVDMR 252
           L  +  L PDPHFK+++H+RRV+   L++     L  GG +   +DV E+   M+
Sbjct: 143 LSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDVEELGEWMK 196

The following BLAST results are available for this feature:

BLAST of MC05g0270.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2JJQ0	1.8e-45	48.60	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-...	[more]
Q2JRH1	1.0e-43	48.34	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX...	[more]
P73161	1.6e-41	44.24	tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / K...	[more]
P72546	3.7e-38	42.59	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 79...	[more]
Q5N2X5	3.7e-38	42.59	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 /...	[more]

BLAST of MC05g0270.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146903.1	1.05e-228	100.00	uncharacterized protein LOC111015991 [Momordica charantia]	[more]
XP_022980361.1	2.68e-193	85.94	uncharacterized protein LOC111479752 [Cucurbita maxima]	[more]
XP_023527130.1	2.20e-192	85.94	uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo]	[more]
KAG7018929.1	3.64e-191	84.98	trmB [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022924717.1	4.24e-190	84.98	uncharacterized protein LOC111432133 [Cucurbita moschata]	[more]

BLAST of MC05g0270.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CZT2	5.06e-229	100.00	uncharacterized protein LOC111015991 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]
A0A6J1ITE1	1.30e-193	85.94	uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752...	[more]
A0A6J1E9S1	2.05e-190	84.98	uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC1114321...	[more]
A0A5A7U1F8	1.05e-176	80.52	tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3AVT2	2.12e-176	80.52	tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 P...	[more]

BLAST of MC05g0270.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17660.1	7.0e-101	72.02	tRNA (guanine-N-7) methyltransferase	[more]
AT5G24840.1	6.3e-09	30.29	tRNA (guanine-N-7) methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 94..310 e-value: 3.3E-54 score: 185.2
None	No IPR available	PANTHER	PTHR23417:SF21	TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE	coord: 62..308
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 127..177 e-value: 0.00192776 score: 35.4835
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PFAM	PF02390	Methyltransf_4	coord: 124..303 e-value: 2.2E-32 score: 111.9
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PANTHER	PTHR23417	3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE	coord: 62..308
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PROSITE	PS51625	SAM_MT_TRMB	coord: 82..308 score: 52.107559
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 123..307

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC05g0270	MC05g0270	gene

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g0270.1-utr3	MC05g0270.1-utr3-MC05:1976387..1976599	utr3

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1976600..1976824	CDS
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1976972..1977070	CDS
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1977170..1977254	CDS
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1977348..1977397	CDS
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1977510..1977589	CDS
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1978477..1978552	CDS
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1978642..1978698	CDS
MC05g0270.1-cds	MC05g0270.1-cds-MC05:1978816..1979076	CDS

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g0270.1-utr5	MC05g0270.1-utr5-MC05:1979077..1979174	utr5

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC05g0270.1	MC05g0270.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006400	tRNA modification
biological_process	GO:0106004	tRNA (guanine-N7)-methylation
cellular_component	GO:0043527	tRNA methyltransferase complex
molecular_function	GO:0008176	tRNA (guanine-N7-)-methyltransferase activity