MC04g1734.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1734.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Membrane-anchored ubiquitin-fold protein
Location	MC04: 24804014 .. 24806071 (-)
Sequence length	381
RNA-Seq Expression	MC04g1734.1
Synteny	MC04g1734.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGAGTGGGAGATCAATTAGAGATCAAGTTTCGATTGAACGATGGATCTGATATCGGTCCTAAAACCTTCCCGGCTGCCACAAGCATCGCAACCTTGAAAGAAAGCATTCTTGCACAATGGCCAAGAGGTGTGTGAAATTTGAACATCTTTCTCATGGTTTGGGCCTTTGGGGGTGTTTGATTTTATTTGAATTCCTATGCTTGAGCTGATCTTGTAGCTCTGTTCGTAATTGAAGTATTAAGTTTTAACATTTTCAGCATATTGGCATCTATTTCGTCATTTACTCAAAATCGGGGAATTTCTTAAGACGAAACATAACACAAGCAAAGCTCAGATCTGATGTAGAGCTCTGCATTTTCTTCATTCTTGCTCTCGCCAATTCAGAAGATACACCACGGAAAAACACCTAACAATCTGTTACCGGAAGTACATCGGTCCATAAAACCTCATGATATTTGTTACATGTTGGATCTTTAATAACCATTTTGATTGAAGTTAGTGACTGGATTTTATGGCTCTTCGAAAGAAAATTCTATCAAGGAATGTCTGATACTCTGTCTGAGTTCTTAACAGAAGAATGCTCTTTGTTTCCGTTTACTGTGGATCTTCTGACTTATTTTCTTCTTCATTTTAACTCAAGTTGCAACATGTTTCTGACATCATTCACCCCAAATTATGTACAGTGGAAATTTGTGGATGTGAACTGGATTGTGGGAGCCTTTCTAGCCCTTCACGCTTCTCGACACCTGCAGTTTTACTCCTGCCATATTCTTTTGTTTATGCATCCTGGTCCTTGAGCGACTAGCTGTCTTGTTTTCTATTGCTTGAATCCATGATTGAACTATATCTCAAAAAGTCTTCTGTTTCTACTTAGCTTTTTAGTTCTGAAATCGAGTAGGCTCAGTTCAGTTAATATCCATAAAGAACATAGTTTCTTGCTGCTCTGCTTTGCTCACTTCAAAGTCATGGGTTCCTTTTTATAGATCTTTCCTGAGGCAGTAATCTTGTAATATCATTCATTACACACAAGTAATTTTCAGTAGGATTTAGTGTCATTCGCATTGGTTTATCGACATATATTGACCATATTGTATATTAATGAGAATTTCAGTGTTAGGCAATGGCAAGAAGGATGTTATTTCTGTTGCATTAGCGCTGGGGCTAGATCTTAATATGCCCTTCTTTACTTTGTTACCGTAATATTGTGAATGTAAGTTTCTTTTGATCAACCATATGATTATTTGTTTGAGCAGAAAAGGAGAATGGTCCAAGAACAGTTAAGGACGTCAAGTTAATAAGTGCAGGAAAAATCTTAGAGAATAACAGAACACTTAATGATTGCAGAAGCCCATTATATGACATTCCTGGAAGTGTGACAACCATGCATGTTGTAATCCAACCACCAAGTTTGGAGAAAGGTATAATTTGTTCTATAAAAAGCTTAAATTATAAATGACTTAAGATAGAATGAATGCTACTTTGGAATTATCTTTGCTGTATTTTGAAGTTTCCTTTGATTCCTTTTGGATCAATAAGAGCTAATTAATCTGATATTTTTGGGGATTTTCCATCACTGTTGATAAGAACTATCTTCTAGAACTTCAGAGTAAGAATTGTTGAATGTGTATACAAAGGTTGCTGGAAATTTGGCCGAATGGCTTTGATCAATCAAACTAATACTTCAAACGCTTAGACATGTTTAGATGTCTTTGCATTGAGCATTGGTATGAATAAGCATATATAATCGTTTGTATGTTGGGTCGAGGGCTGTGGAAATACGATAGGAAGTTTGTATCAAACACAACAACCAGTTAATTGTGTTAAGTTTAAACTAGTTTCGAGTACTATGCGAAAAGATAAACTCTCAATTTAAGGGAACGGTTTGCTTGTATCTGAAAACCCTTCTGGCTGTTGCTTCAATGGGATTACTGAGTAAGATTTATATTCTTTCTTTGCCTACGCAGAAAAGAAAGCTGGAGATCAGTCAACACAAAACAAGTGTGTTTGTGTTATACTATAAACCGAACTCATGGAAGTTCTCGACGCCATTTT

mRNA sequence

ATGGCTGGAGTGGGAGATCAATTAGAGATCAAGTTTCGATTGAACGATGGATCTGATATCGGTCCTAAAACCTTCCCGGCTGCCACAAGCATCGCAACCTTGAAAGAAAGCATTCTTGCACAATGGCCAAGAGAAAAGGAGAATGGTCCAAGAACAGTTAAGGACGTCAAGTTAATAAGTGCAGGAAAAATCTTAGAGAATAACAGAACACTTAATGATTGCAGAAGCCCATTATATGACATTCCTGGAAGTGTGACAACCATGCATGTTGTAATCCAACCACCAAGTTTGGAGAAAGAAAGCTGGAGATCAGTCAACACAAAACAAGTGTGTTTGTGTTATACTATAAACCGAACTCATGGAAGTTCTCGACGCCATTTT

Coding sequence (CDS)

ATGGCTGGAGTGGGAGATCAATTAGAGATCAAGTTTCGATTGAACGATGGATCTGATATCGGTCCTAAAACCTTCCCGGCTGCCACAAGCATCGCAACCTTGAAAGAAAGCATTCTTGCACAATGGCCAAGAGAAAAGGAGAATGGTCCAAGAACAGTTAAGGACGTCAAGTTAATAAGTGCAGGAAAAATCTTAGAGAATAACAGAACACTTAATGATTGCAGAAGCCCATTATATGACATTCCTGGAAGTGTGACAACCATGCATGTTGTAATCCAACCACCAAGTTTGGAGAAAGAAAGCTGGAGATCAGTCAACACAAAACAAGTGTGTTTGTGTTATACTATAAACCGAACTCATGGAAGTTCTCGACGCCATTTT

Protein sequence

MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLISAGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVNTKQVCLCYTINRTHGSSRRHF

Homology

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BLAST of MC04g1734.1 vs. ExPASy Swiss-Prot
Match: Q8LCS8 (Membrane-anchored ubiquitin-fold protein 2 OS=Arabidopsis thaliana OX=3702 GN=MUB2 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.4e-39
Identity = 79/120 (65.83%), Postives = 95/120 (79.17%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MA V DQLEIKFRLNDGSDIGPK FP AT++ATLKE+++AQWPR+KENGP+TVKDVKLIS
Sbjct: 1   MAEVKDQLEIKFRLNDGSDIGPKLFPDATTVATLKETVVAQWPRDKENGPKTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRS------VNTKQVCLCY 115
           AG+ILENN+T+ DCRSP+ +  G+VTTMHV+IQ    EKE  +          K VCLC+
Sbjct: 61  AGRILENNKTVGDCRSPVGNFSGAVTTMHVIIQHQVTEKEKKKKKPKGDLKQNKCVCLCF 120

[Close]

BLAST of MC04g1734.1 vs. ExPASy Swiss-Prot
Match: Q9MAB9 (Membrane-anchored ubiquitin-fold protein 1 OS=Arabidopsis thaliana OX=3702 GN=MUB1 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-35
Identity = 70/114 (61.40%), Postives = 92/114 (80.70%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MA V +QLEIKFRL DGSDIGPK FP AT+++ LKE+++++WPREKENGP+TVK+VKLIS
Sbjct: 1   MAEVHNQLEIKFRLTDGSDIGPKAFPDATTVSALKETVISEWPREKENGPKTVKEVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVNTK-QVCLC 114
           AGK+LEN++T+ D RSP+ ++ G+VTTMHV+IQ P  EKE     + K   C+C
Sbjct: 61  AGKVLENSKTVKDYRSPVSNLAGAVTTMHVIIQAPVTEKEKKPKGDPKMNKCVC 114

[Close]

BLAST of MC04g1734.1 vs. ExPASy Swiss-Prot
Match: Q75GT2 (Membrane-anchored ubiquitin-fold protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MUB1 PE=3 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 6.6e-34
Identity = 67/106 (63.21%), Postives = 90/106 (84.91%), Query Frame = 0


Query: 3   GVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLISAG 62
           GV +Q EIKFRL DG+DIGPK +PAA+++ATLKESI+AQWP++KE GPRTV D+KLI+AG
Sbjct: 4   GVQEQFEIKFRLPDGTDIGPKRYPAASTVATLKESIVAQWPKDKEKGPRTVNDLKLINAG 63

Query: 63  KILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVNTK 109
           KILENN+TL++C+SP+ D  G +TTMHVV++ P+ +K+S + V  K
Sbjct: 64  KILENNKTLSECKSPICDFSG-LTTMHVVVRAPTSDKQSNKIVAKK 108

[Close]

BLAST of MC04g1734.1 vs. ExPASy Swiss-Prot
Match: Q8GWJ6 (Membrane-anchored ubiquitin-fold protein 6 OS=Arabidopsis thaliana OX=3702 GN=MUB6 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-29
Identity = 58/116 (50.00%), Postives = 88/116 (75.86%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAG  D +E+KFRL DG+DIGP  +  + ++++LKE +++QWP++KEN P+TV D+KLI+
Sbjct: 1   MAGEEDWIELKFRLADGTDIGPSKYNQSMTVSSLKEKLISQWPKDKENTPKTVNDMKLIN 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVN---TKQVCLC 114
           AGKILENNRTL + R P+ ++PG + TMH+V++ P+L+K+S +  N    K  C+C
Sbjct: 61  AGKILENNRTLAESRLPVCELPGMIITMHIVLRLPTLDKKSEKLQNDPPMKNRCVC 116

[Close]

BLAST of MC04g1734.1 vs. ExPASy Swiss-Prot
Match: Q6Z8K4 (Membrane-anchored ubiquitin-fold protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MUB3 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.1e-28
Identity = 56/101 (55.45%), Postives = 79/101 (78.22%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAG  + +E+KFRL DG+DIGP  +  +T+++ LKE ILA+WP++KE  P+TV D+KLI+
Sbjct: 1   MAGGKEPIEVKFRLFDGTDIGPSKYDPSTTVSALKEFILARWPQDKEITPKTVNDLKLIN 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKES 102
           AG+ILENNRTL + R P+ ++PG V TMHVV++PP  +K S
Sbjct: 61  AGRILENNRTLAESRVPVGEVPGGVITMHVVVRPPQPDKNS 101

[Close]

BLAST of MC04g1734.1 vs. NCBI nr
Match: XP_008459365.1 (PREDICTED: membrane-anchored ubiquitin-fold protein 1-like isoform X1 [Cucumis melo] >XP_008459366.1 PREDICTED: membrane-anchored ubiquitin-fold protein 1-like isoform X1 [Cucumis melo] >XP_008459367.1 PREDICTED: membrane-anchored ubiquitin-fold protein 1-like isoform X1 [Cucumis melo] >XP_008459368.1 PREDICTED: membrane-anchored ubiquitin-fold protein 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 210 bits (534), Expect = 1.05e-67
Identity = 105/117 (89.74%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEK-ESWRSVNTKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPP+LEK ES  +++ K  C CY I
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPTLEKVESVPNMSVKNCCGCYYI 117

[Close]

BLAST of MC04g1734.1 vs. NCBI nr
Match: XP_022134039.1 (membrane-anchored ubiquitin-fold protein 2-like [Momordica charantia] >XP_022134040.1 membrane-anchored ubiquitin-fold protein 2-like [Momordica charantia] >XP_022134041.1 membrane-anchored ubiquitin-fold protein 2-like [Momordica charantia] >XP_022134042.1 membrane-anchored ubiquitin-fold protein 2-like [Momordica charantia] >XP_022134043.1 membrane-anchored ubiquitin-fold protein 2-like [Momordica charantia])

HSP 1 Score: 207 bits (526), Expect = 1.35e-66
Identity = 103/117 (88.03%), Postives = 107/117 (91.45%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRS-VNTKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKE      +T+  C+C  +
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKEKKAGDQSTQNKCVCVIL 117

[Close]

BLAST of MC04g1734.1 vs. NCBI nr
Match: XP_038889784.1 (membrane-anchored ubiquitin-fold protein 2-like [Benincasa hispida] >XP_038889785.1 membrane-anchored ubiquitin-fold protein 2-like [Benincasa hispida] >XP_038889786.1 membrane-anchored ubiquitin-fold protein 2-like [Benincasa hispida] >XP_038889787.1 membrane-anchored ubiquitin-fold protein 2-like [Benincasa hispida])

HSP 1 Score: 206 bits (524), Expect = 2.72e-66
Identity = 103/117 (88.03%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVN-TKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKE       T+  C+C  +
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKEKKAGDQATQNKCVCVIL 117

[Close]

BLAST of MC04g1734.1 vs. NCBI nr
Match: XP_008459370.1 (PREDICTED: membrane-anchored ubiquitin-fold protein 2-like isoform X2 [Cucumis melo] >XP_008459372.1 PREDICTED: membrane-anchored ubiquitin-fold protein 2-like isoform X2 [Cucumis melo])

HSP 1 Score: 205 bits (522), Expect = 5.49e-66
Identity = 102/117 (87.18%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVN-TKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPP+LEKE       T+  C+C  +
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPTLEKEKKAGEQATQNKCVCVIL 117

[Close]

BLAST of MC04g1734.1 vs. NCBI nr
Match: XP_004141460.1 (membrane-anchored ubiquitin-fold protein 2 [Cucumis sativus] >XP_011656006.1 membrane-anchored ubiquitin-fold protein 2 [Cucumis sativus] >XP_031741332.1 membrane-anchored ubiquitin-fold protein 2 [Cucumis sativus] >XP_031741333.1 membrane-anchored ubiquitin-fold protein 2 [Cucumis sativus] >KGN52499.1 hypothetical protein Csa_008602 [Cucumis sativus])

HSP 1 Score: 205 bits (521), Expect = 7.79e-66
Identity = 101/117 (86.32%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATS+ATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSVATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVN-TKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPP+LEKE       T+  C+C  +
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPTLEKEKKAGEQATQNKCVCVIL 117

[Close]

BLAST of MC04g1734.1 vs. ExPASy TrEMBL
Match: A0A1S3CA36 (Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103498522 PE=4 SV=1)

HSP 1 Score: 210 bits (534), Expect = 5.10e-68
Identity = 105/117 (89.74%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEK-ESWRSVNTKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPP+LEK ES  +++ K  C CY I
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPTLEKVESVPNMSVKNCCGCYYI 117

[Close]

BLAST of MC04g1734.1 vs. ExPASy TrEMBL
Match: A0A6J1BXN4 (Membrane-anchored ubiquitin-fold protein OS=Momordica charantia OX=3673 GN=LOC111006411 PE=4 SV=1)

HSP 1 Score: 207 bits (526), Expect = 6.52e-67
Identity = 103/117 (88.03%), Postives = 107/117 (91.45%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRS-VNTKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKE      +T+  C+C  +
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKEKKAGDQSTQNKCVCVIL 117

[Close]

BLAST of MC04g1734.1 vs. ExPASy TrEMBL
Match: A0A1S3CB88 (Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103498522 PE=4 SV=1)

HSP 1 Score: 205 bits (522), Expect = 2.66e-66
Identity = 102/117 (87.18%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVN-TKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPP+LEKE       T+  C+C  +
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPTLEKEKKAGEQATQNKCVCVIL 117

[Close]

BLAST of MC04g1734.1 vs. ExPASy TrEMBL
Match: A0A0A0KUE8 (Membrane-anchored ubiquitin-fold protein OS=Cucumis sativus OX=3659 GN=Csa_5G638380 PE=4 SV=1)

HSP 1 Score: 205 bits (521), Expect = 3.77e-66
Identity = 101/117 (86.32%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATS+ATLKESILAQWPREKENGPRTVKDVKLIS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSVATLKESILAQWPREKENGPRTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVN-TKQVCLCYTI 116
           AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPP+LEKE       T+  C+C  +
Sbjct: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPTLEKEKKAGEQATQNKCVCVIL 117

[Close]

BLAST of MC04g1734.1 vs. ExPASy TrEMBL
Match: A0A6J1JRG7 (Membrane-anchored ubiquitin-fold protein OS=Cucurbita maxima OX=3661 GN=LOC111487674 PE=4 SV=1)

HSP 1 Score: 203 bits (516), Expect = 2.18e-65
Identity = 100/114 (87.72%), Postives = 105/114 (92.11%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKES+LAQWPREKENGPRTVKDVK+IS
Sbjct: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESVLAQWPREKENGPRTVKDVKIIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVNTKQ-VCLC 113
           AG+ILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKE      + Q  C+C
Sbjct: 61  AGRILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKEKKAGDQSPQNKCVC 114

[Close]

BLAST of MC04g1734.1 vs. TAIR 10
Match: AT5G15460.1 (membrane-anchored ubiquitin-fold protein 2 )

HSP 1 Score: 163.7 bits (413), Expect = 9.7e-41
Identity = 79/120 (65.83%), Postives = 95/120 (79.17%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MA V DQLEIKFRLNDGSDIGPK FP AT++ATLKE+++AQWPR+KENGP+TVKDVKLIS
Sbjct: 1   MAEVKDQLEIKFRLNDGSDIGPKLFPDATTVATLKETVVAQWPRDKENGPKTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRS------VNTKQVCLCY 115
           AG+ILENN+T+ DCRSP+ +  G+VTTMHV+IQ    EKE  +          K VCLC+
Sbjct: 61  AGRILENNKTVGDCRSPVGNFSGAVTTMHVIIQHQVTEKEKKKKKPKGDLKQNKCVCLCF 120

[Close]

BLAST of MC04g1734.1 vs. TAIR 10
Match: AT5G15460.2 (membrane-anchored ubiquitin-fold protein 2 )

HSP 1 Score: 163.7 bits (413), Expect = 9.7e-41
Identity = 79/120 (65.83%), Postives = 95/120 (79.17%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MA V DQLEIKFRLNDGSDIGPK FP AT++ATLKE+++AQWPR+KENGP+TVKDVKLIS
Sbjct: 1   MAEVKDQLEIKFRLNDGSDIGPKLFPDATTVATLKETVVAQWPRDKENGPKTVKDVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRS------VNTKQVCLCY 115
           AG+ILENN+T+ DCRSP+ +  G+VTTMHV+IQ    EKE  +          K VCLC+
Sbjct: 61  AGRILENNKTVGDCRSPVGNFSGAVTTMHVIIQHQVTEKEKKKKKPKGDLKQNKCVCLCF 120

[Close]

BLAST of MC04g1734.1 vs. TAIR 10
Match: AT3G01050.1 (membrane-anchored ubiquitin-fold protein 1 precursor )

HSP 1 Score: 150.2 bits (378), Expect = 1.1e-36
Identity = 70/114 (61.40%), Postives = 92/114 (80.70%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MA V +QLEIKFRL DGSDIGPK FP AT+++ LKE+++++WPREKENGP+TVK+VKLIS
Sbjct: 1   MAEVHNQLEIKFRLTDGSDIGPKAFPDATTVSALKETVISEWPREKENGPKTVKEVKLIS 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVNTK-QVCLC 114
           AGK+LEN++T+ D RSP+ ++ G+VTTMHV+IQ P  EKE     + K   C+C
Sbjct: 61  AGKVLENSKTVKDYRSPVSNLAGAVTTMHVIIQAPVTEKEKKPKGDPKMNKCVC 114

[Close]

BLAST of MC04g1734.1 vs. TAIR 10
Match: AT1G22050.1 (membrane-anchored ubiquitin-fold protein 6 precursor )

HSP 1 Score: 130.6 bits (327), Expect = 9.1e-31
Identity = 58/116 (50.00%), Postives = 88/116 (75.86%), Query Frame = 0


Query: 1   MAGVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLIS 60
           MAG  D +E+KFRL DG+DIGP  +  + ++++LKE +++QWP++KEN P+TV D+KLI+
Sbjct: 1   MAGEEDWIELKFRLADGTDIGPSKYNQSMTVSSLKEKLISQWPKDKENTPKTVNDMKLIN 60

Query: 61  AGKILENNRTLNDCRSPLYDIPGSVTTMHVVIQPPSLEKESWRSVN---TKQVCLC 114
           AGKILENNRTL + R P+ ++PG + TMH+V++ P+L+K+S +  N    K  C+C
Sbjct: 61  AGKILENNRTLAESRLPVCELPGMIITMHIVLRLPTLDKKSEKLQNDPPMKNRCVC 116

[Close]

BLAST of MC04g1734.1 vs. TAIR 10
Match: AT1G77870.1 (membrane-anchored ubiquitin-fold protein 5 precursor )

HSP 1 Score: 122.9 bits (307), Expect = 1.9e-28
Identity = 58/116 (50.00%), Postives = 83/116 (71.55%), Query Frame = 0


Query: 3   GVGDQLEIKFRLNDGSDIGPKTFPAATSIATLKESILAQWPREKENGPRTVKDVKLISAG 62
           G  D +E+KFRL DG+DIGP  +    ++A+LKE I+AQWP++KEN P+ + +VKLI+ G
Sbjct: 2   GDEDLIELKFRLADGTDIGPSKYSQFMTVASLKEKIIAQWPKDKENAPKMINEVKLINGG 61

Query: 63  KILENNRTLNDCRS--PLYDIPGSVTTMHVVIQPPSLEKESWRSVN---TKQVCLC 114
           KILENN+TL++ RS   + ++PG VTTMHVV++PP  EK+  +  N    K  C+C
Sbjct: 62  KILENNKTLSEARSLITIGELPGIVTTMHVVLRPPLFEKKKEKLQNDPPRKSHCVC 117

The following BLAST results are available for this feature:

BLAST of MC04g1734.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LCS8	1.4e-39	65.83	Membrane-anchored ubiquitin-fold protein 2 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q9MAB9	1.6e-35	61.40	Membrane-anchored ubiquitin-fold protein 1 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q75GT2	6.6e-34	63.21	Membrane-anchored ubiquitin-fold protein 1 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q8GWJ6	1.3e-29	50.00	Membrane-anchored ubiquitin-fold protein 6 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q6Z8K4	4.1e-28	55.45	Membrane-anchored ubiquitin-fold protein 3 OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of MC04g1734.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459365.1	1.05e-67	89.74	PREDICTED: membrane-anchored ubiquitin-fold protein 1-like isoform X1 [Cucumis m...	[more]
XP_022134039.1	1.35e-66	88.03	membrane-anchored ubiquitin-fold protein 2-like [Momordica charantia] >XP_022134...	[more]
XP_038889784.1	2.72e-66	88.03	membrane-anchored ubiquitin-fold protein 2-like [Benincasa hispida] >XP_03888978...	[more]
XP_008459370.1	5.49e-66	87.18	PREDICTED: membrane-anchored ubiquitin-fold protein 2-like isoform X2 [Cucumis m...	[more]
XP_004141460.1	7.79e-66	86.32	membrane-anchored ubiquitin-fold protein 2 [Cucumis sativus] >XP_011656006.1 mem...	[more]

BLAST of MC04g1734.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CA36	5.10e-68	89.74	Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103498522...	[more]
A0A6J1BXN4	6.52e-67	88.03	Membrane-anchored ubiquitin-fold protein OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A1S3CB88	2.66e-66	87.18	Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103498522...	[more]
A0A0A0KUE8	3.77e-66	86.32	Membrane-anchored ubiquitin-fold protein OS=Cucumis sativus OX=3659 GN=Csa_5G638...	[more]
A0A6J1JRG7	2.18e-65	87.72	Membrane-anchored ubiquitin-fold protein OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]

BLAST of MC04g1734.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15460.1	9.7e-41	65.83	membrane-anchored ubiquitin-fold protein 2	[more]
AT5G15460.2	9.7e-41	65.83	membrane-anchored ubiquitin-fold protein 2	[more]
AT3G01050.1	1.1e-36	61.40	membrane-anchored ubiquitin-fold protein 1 precursor	[more]
AT1G22050.1	9.1e-31	50.00	membrane-anchored ubiquitin-fold protein 6 precursor	[more]
AT1G77870.1	1.9e-28	50.00	membrane-anchored ubiquitin-fold protein 5 precursor	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017000	Membrane-anchored ubiquitin-fold protein	PIRSF	PIRSF032572	MUB	coord: 1..116 e-value: 1.2E-43 score: 145.8
None	No IPR available	GENE3D	3.10.20.90		coord: 1..101 e-value: 4.4E-40 score: 137.8
None	No IPR available	PANTHER	PTHR13169:SF4	MEMBRANE-ANCHORED UBIQUITIN-FOLD PROTEIN 2	coord: 1..113
None	No IPR available	CDD	cd01814	Ubl_MUBs_plant	coord: 7..95 e-value: 3.14011E-51 score: 155.23
IPR039540	UBL3-like, ubiquitin domain	PFAM	PF13881	Rad60-SLD_2	coord: 6..109 e-value: 1.7E-38 score: 131.2
IPR040015	UBL3-like	PANTHER	PTHR13169	UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN	coord: 1..113
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 8..74 score: 8.676706
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 4..99

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC04g1734	MC04g1734	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC04g1734.1-cds	MC04g1734.1-cds-MC04:24804014..24804096	CDS
MC04g1734.1-cds	MC04g1734.1-cds-MC04:24804648..24804812	CDS
MC04g1734.1-cds	MC04g1734.1-cds-MC04:24805939..24806071	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC04g1734.1	MC04g1734.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005515	protein binding