MC04g1623.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC04g1623.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	sirohydrochlorin ferrochelatase, chloroplastic
Location	MC04: 23731269 .. 23732725 (+)
Sequence length	555
RNA-Seq Expression	MC04g1623.1
Synteny	MC04g1623.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TACAAAATCTGCGCATCTCAGAAATCTTCTGTTCCGATCGGACGTTCCTCTAGATTCAGAAATTTCGCCTCGCGGTTCCGCATGAGTGTTGATAATGGAGGCGCTAATAGGAGCGGAGCTCGGATTGGTTCTGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGCCAGGAATCTAACCTCATGCTGAGTAAGCTAGGGTTTTGGAATTTCGAATTTGCTTGAAATTTTTTTGAAGTTCTATGCGTCTAATCCACTTGTGGCTCCGAATTTCTTGAAAATTTCAGATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATAGTGGAGCCTGCCCACATGGTATGTTCTTTTAACTGTTTTTTCACTTGTTCATCCATATATTTTAGTTTTGAATTATCCTACATTTTGTATTACGTTCATCTCTGTTCCCTATTTTTAACATCCGTAAGCAGTAACGGAGAATATTGTTTCATCCTTAATATTTATGTTGAACCCTTCAACTTATGAAAATATCAATCTACTCTTAAGTACAGCATCACGAACATTCAAAATAACATGGACCCAACCCAATAAATAATGATATCTTTAAATATGAATAGTATAGTAGTACATAACCTCCCCCCTCTCCCGTTTTTTAATAATTGAGAGTCGCTCTCCTATGCTCAGGGGCGGAAATTATTCTATTCTATCGAGCTACTAGAAGTTTCTTTTTCATCGTCTTTTTCTTCTGGTCCCGTGCACGCATGTTTGAACACAACACATGATGTTAACTGATTTGATTAGAAAGAGTAACAGTCACAACCACGTCTAATGTACTTGTGCTTTCTTGGTCTATTCTTAGCTATTATTGGTGTGGCCGGCCGGGGGCTCTAATGATTTTTCTTCTACTCTGTTCTGAAACTACACAGGAGCTGGCCGAACCGTCGATAAGAGACTCATTTGCATCGTGTGTTGAACAGGGGGCGAAACATATCATTGTAAGCCCATTCTTCCTCTTTCCCGGCCGACATTGGCAACAGGTAGCTCACTGTAAGATGGTTAATTTGATGCGATGAACCAGTGTCTGTTTCATCTTCTGTGACTTCCAGCAACAGGATTGATAGAAATGGCTGAAATTTTGCAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCATATATAGTAACAGCTCCTCTTGGTCTTCATGAAAAACTTGTGGTAAGAATTTCTCAAGTGGACTTTTTTCCAGTACAGGATAATGAAGAAAGCAAGGGCATACTTCATAGACTCATAAGTGATAAAAGAAAAAAATATATTTGTTCGAATTAAACTTGGGTATTCTGTTCGCAGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGTGCTGGAACAGGCAAATGCAGATTT

mRNA sequence

TACAAAATCTGCGCATCTCAGAAATCTTCTGTTCCGATCGGACGTTCCTCTAGATTCAGAAATTTCGCCTCGCGGTTCCGCATGAGTGTTGATAATGGAGGCGCTAATAGGAGCGGAGCTCGGATTGGTTCTGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGCCAGGAATCTAACCTCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATAGTGGAGCCTGCCCACATGGAGCTGGCCGAACCGTCGATAAGAGACTCATTTGCATCGTGTGTTGAACAGGGGGCGAAACATATCATTGTAAGCCCATTCTTCCTCTTTCCCGGCCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCATATATAGTAACAGCTCCTCTTGGTCTTCATGAAAAACTTGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGTGCTGGAACAGGCAAATGCAGATTT

Coding sequence (CDS)

TACAAAATCTGCGCATCTCAGAAATCTTCTGTTCCGATCGGACGTTCCTCTAGATTCAGAAATTTCGCCTCGCGGTTCCGCATGAGTGTTGATAATGGAGGCGCTAATAGGAGCGGAGCTCGGATTGGTTCTGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGCCAGGAATCTAACCTCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATAGTGGAGCCTGCCCACATGGAGCTGGCCGAACCGTCGATAAGAGACTCATTTGCATCGTGTGTTGAACAGGGGGCGAAACATATCATTGTAAGCCCATTCTTCCTCTTTCCCGGCCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCATATATAGTAACAGCTCCTCTTGGTCTTCATGAAAAACTTGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGTGCTGGAACAGGCAAATGCAGATTT

Protein sequence

YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGTGKCRF

Homology

[Close]

BLAST of MC04g1623.1 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-61
Identity = 107/143 (74.83%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 42  IGSGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVE 101
           IG  D +IIVDHGSRR+ESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ CV+
Sbjct: 78  IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLCVQ 137

Query: 102 QGAKHIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRIN 161
           QGAK ++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY++TAPLG H  L+DVVNDRI 
Sbjct: 138 QGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDRIQ 197

Query: 162 HCLSHAAGVADECEVCAGTGKCR 185
           HCLSH  G ADEC VCAGT KC+
Sbjct: 198 HCLSHVEGDADECLVCAGTNKCK 220

[Close]

BLAST of MC04g1623.1 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 40/116 (34.48%), Postives = 61/116 (52.59%), Query Frame = 0


Query: 46  DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 105
           DAV+ V HGSR +E       F+   +     PI E   +ELA P+I   F +C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 106 HIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRIN 162
            I + P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +RIN
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVERIN 128

[Close]

BLAST of MC04g1623.1 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.5e-13
Identity = 41/141 (29.08%), Postives = 66/141 (46.81%), Query Frame = 0


Query: 46  DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 105
           +A+++V HGSR   S  ++ E     + +  Y IVE   ME  EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 106 HIIVSPFFLFPGRHWQQDIPSLTA------EAAKDH-------------------PGISY 162
            +IV+P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]

BLAST of MC04g1623.1 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 42/138 (30.43%), Postives = 65/138 (47.10%), Query Frame = 0


Query: 46  DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 105
           +A+++V HGSR   S  +L +     +++  +PIVE   ME +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 106 HIIVSPFFLFPGRHWQQDIPSLTAEAAKDH------------------------PGISYI 160
            IIV P FL  G H  +DIP L      +H                          +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

[Close]

BLAST of MC04g1623.1 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.0e-11
Identity = 40/138 (28.99%), Postives = 65/138 (47.10%), Query Frame = 0


Query: 46  DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 105
           +A++++ HGSR   S  ++ +     ++K  Y IVE   ME  EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 106 HIIVSPFFLFPGRHWQQDIPSLTA--EAAKDH----------------------PGISYI 160
            II+ P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

[Close]

BLAST of MC04g1623.1 vs. NCBI nr
Match: XP_022134499.1 (sirohydrochlorin ferrochelatase, chloroplastic [Momordica charantia])

HSP 1 Score: 377 bits (967), Expect = 1.93e-131
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES
Sbjct: 24  YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 83

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW
Sbjct: 84  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 143

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT
Sbjct: 144 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 203

Query: 181 GKCRF 185
           GKCRF
Sbjct: 204 GKCRF 208

[Close]

BLAST of MC04g1623.1 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 343 bits (881), Expect = 9.10e-118
Identity = 168/185 (90.81%), Postives = 175/185 (94.59%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           Y ICASQKS + I R SRFRNF SRFRMSVDNG +NRSG RIG+GDAVIIVDHGSRR+ES
Sbjct: 61  YTICASQKSPILIRRLSRFRNFTSRFRMSVDNGSSNRSGDRIGAGDAVIIVDHGSRRRES 120

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFA CVEQGAK IIVSPFFLFPGRHW
Sbjct: 121 NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFALCVEQGAKRIIVSPFFLFPGRHW 180

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVVNDRI+HCLSHAAGVA+ECEVCAGT
Sbjct: 181 QQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRISHCLSHAAGVAEECEVCAGT 240

Query: 181 GKCRF 185
           GKCRF
Sbjct: 241 GKCRF 245

[Close]

BLAST of MC04g1623.1 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 337 bits (863), Expect = 1.20e-115
Identity = 164/185 (88.65%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           Y ICA Q+S +PIGR S+FRNF SR RMS+DNG +NR    IG+GDAVIIVDHGSRR+ES
Sbjct: 24  YTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIVDHGSRRRES 83

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAK IIVSPFFLFPGRHW
Sbjct: 84  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFPGRHW 143

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT
Sbjct: 144 QQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 203

Query: 181 GKCRF 185
           GKCRF
Sbjct: 204 GKCRF 204

[Close]

BLAST of MC04g1623.1 vs. NCBI nr
Match: XP_008458790.1 (PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo])

HSP 1 Score: 337 bits (863), Expect = 1.20e-115
Identity = 164/185 (88.65%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           Y ICASQ+S +PIGR S+FRNF SR RMS+DNG +NR    IG+GDAVIIVDHGSRR+ES
Sbjct: 24  YTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIVDHGSRRRES 83

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           N MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAK IIVSPFFLFPGRHW
Sbjct: 84  NRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFPGRHW 143

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT
Sbjct: 144 QQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 203

Query: 181 GKCRF 185
           GKCRF
Sbjct: 204 GKCRF 204

[Close]

BLAST of MC04g1623.1 vs. NCBI nr
Match: KAG7033101.1 (Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336 bits (861), Expect = 2.88e-115
Identity = 164/185 (88.65%), Postives = 173/185 (93.51%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           Y I  SQKS++PIGR SRF NF SRFRMSVDNGG+++S  RIG+GDAVIIVDHGSRRQES
Sbjct: 25  YTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSSQSRDRIGAGDAVIIVDHGSRRQES 84

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           NLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F SCVEQGAK II+SPFFLFPGRHW
Sbjct: 85  NLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAKRIIISPFFLFPGRHW 144

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT
Sbjct: 145 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 204

Query: 181 GKCRF 185
           GKCRF
Sbjct: 205 GKCRF 209

[Close]

BLAST of MC04g1623.1 vs. ExPASy TrEMBL
Match: A0A6J1BYW9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006726 PE=4 SV=1)

HSP 1 Score: 377 bits (967), Expect = 9.36e-132
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES
Sbjct: 24  YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 83

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW
Sbjct: 84  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 143

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT
Sbjct: 144 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 203

Query: 181 GKCRF 185
           GKCRF
Sbjct: 204 GKCRF 208

[Close]

BLAST of MC04g1623.1 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 337 bits (863), Expect = 5.80e-116
Identity = 164/185 (88.65%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           Y ICA Q+S +PIGR S+FRNF SR RMS+DNG +NR    IG+GDAVIIVDHGSRR+ES
Sbjct: 24  YTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIVDHGSRRRES 83

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAK IIVSPFFLFPGRHW
Sbjct: 84  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFPGRHW 143

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT
Sbjct: 144 QQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 203

Query: 181 GKCRF 185
           GKCRF
Sbjct: 204 GKCRF 204

[Close]

BLAST of MC04g1623.1 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 337 bits (863), Expect = 5.80e-116
Identity = 164/185 (88.65%), Postives = 172/185 (92.97%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           Y ICASQ+S +PIGR S+FRNF SR RMS+DNG +NR    IG+GDAVIIVDHGSRR+ES
Sbjct: 24  YTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIVDHGSRRRES 83

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           N MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAK IIVSPFFLFPGRHW
Sbjct: 84  NRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFPGRHW 143

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT
Sbjct: 144 QQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 203

Query: 181 GKCRF 185
           GKCRF
Sbjct: 204 GKCRF 204

[Close]

BLAST of MC04g1623.1 vs. ExPASy TrEMBL
Match: A0A0A0KP17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1)

HSP 1 Score: 332 bits (852), Expect = 2.75e-114
Identity = 159/185 (85.95%), Postives = 174/185 (94.05%), Query Frame = 0


Query: 1   YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQES 60
           + ICASQ+SS+P+GR S+FRNF SRFRMS+DNG +NR    IG+GDAVIIVDHGSRR+ES
Sbjct: 24  FSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR----IGAGDAVIIVDHGSRRRES 83

Query: 61  NLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGRHW 120
           NLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSF  CVEQGAK IIVSPFFLFPGRHW
Sbjct: 84  NLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAKRIIVSPFFLFPGRHW 143

Query: 121 QQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGT 180
           QQDIPSLTAEAAKDHPGISYI+TAPLGLHE+LVDVVNDRIN+CLSHAAGV+DECEVCAGT
Sbjct: 144 QQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINYCLSHAAGVSDECEVCAGT 203

Query: 181 GKCRF 185
           GKCRF
Sbjct: 204 GKCRF 204

[Close]

BLAST of MC04g1623.1 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 332 bits (850), Expect = 5.95e-114
Identity = 164/187 (87.70%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   YKICASQKSSV--PIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDHGSRRQ 60
           Y ICASQKSS+   IGRSS  RNF+SRFRMSVDNGG+NR    IG+GDAVIIVDHGSRR+
Sbjct: 24  YTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGSNR----IGAGDAVIIVDHGSRRR 83

Query: 61  ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFFLFPGR 120
           ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK I+VSPFFLFPGR
Sbjct: 84  ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVVSPFFLFPGR 143

Query: 121 HWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCA 180
           HWQQDIPSLTAEAAKDHPGISYI+TAPLGLHE+LVDVVNDRI+HCLSHAAGV +ECEVCA
Sbjct: 144 HWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAGVVEECEVCA 203

Query: 181 GTGKCRF 185
           GTGKC+F
Sbjct: 204 GTGKCKF 206

[Close]

BLAST of MC04g1623.1 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 237.7 bits (605), Expect = 7.7e-63
Identity = 107/143 (74.83%), Postives = 123/143 (86.01%), Query Frame = 0


Query: 42  IGSGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVE 101
           IG  D +IIVDHGSRR+ESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ CV+
Sbjct: 78  IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLCVQ 137

Query: 102 QGAKHIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRIN 161
           QGAK ++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY++TAPLG H  L+DVVNDRI 
Sbjct: 138 QGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDRIQ 197

Query: 162 HCLSHAAGVADECEVCAGTGKCR 185
           HCLSH  G ADEC VCAGT KC+
Sbjct: 198 HCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:

BLAST of MC04g1623.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84JH7	1.1e-61	74.83	Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
P61817	1.5e-15	34.48	Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1	[more]
P61819	2.5e-13	29.08	Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ...	[more]
Q58380	1.6e-12	30.43	Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ...	[more]
A6UWT5	3.0e-11	28.99	Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128...	[more]

BLAST of MC04g1623.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134499.1	1.93e-131	100.00	sirohydrochlorin ferrochelatase, chloroplastic [Momordica charantia]	[more]
XP_038890885.1	9.10e-118	90.81	sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida]	[more]
KAA0037556.1	1.20e-115	88.65	sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro...	[more]
XP_008458790.1	1.20e-115	88.65	PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo]	[more]
KAG7033101.1	2.88e-115	88.65	Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma ...	[more]

BLAST of MC04g1623.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYW9	9.36e-132	100.00	sirohydrochlorin ferrochelatase, chloroplastic OS=Momordica charantia OX=3673 GN...	[more]
A0A5D3BY55	5.80e-116	88.65	Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3C8S9	5.80e-116	88.65	sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A0A0KP17	2.75e-114	85.95	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1	[more]
A0A6J1K384	5.95e-114	87.70	sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ...	[more]

BLAST of MC04g1623.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50170.1	7.7e-63	74.83	sirohydrochlorin ferrochelatase B	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002762	Sirohydrochlorin cobaltochelatase CbiX-like	PFAM	PF01903	CbiX	coord: 53..157 e-value: 2.2E-30 score: 105.1
None	No IPR available	GENE3D	3.40.50.1400		coord: 44..167 e-value: 1.2E-34 score: 121.2
None	No IPR available	PANTHER	PTHR33542:SF3	SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC	coord: 36..183
None	No IPR available	PANTHER	PTHR33542	SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC	coord: 36..183
None	No IPR available	CDD	cd03416	CbiX_SirB_N	coord: 47..147 e-value: 5.55864E-38 score: 124.606
None	No IPR available	SUPERFAMILY	53800	Chelatase	coord: 45..163

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC04g1623	MC04g1623	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC04g1623.1-cds	MC04g1623.1-cds-MC04:23731269..23731461	CDS
MC04g1623.1-cds	MC04g1623.1-cds-MC04:23731559..23731620	CDS
MC04g1623.1-cds	MC04g1623.1-cds-MC04:23732190..23732300	CDS
MC04g1623.1-cds	MC04g1623.1-cds-MC04:23732405..23732497	CDS
MC04g1623.1-cds	MC04g1623.1-cds-MC04:23732630..23732725	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC04g1623.1	MC04g1623.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0019354	siroheme biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0042802	identical protein binding
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051266	sirohydrochlorin ferrochelatase activity