MC03g0139.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0139.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pentatricopeptide repeat-containing protein
Location	MC03: 2383903 .. 2387857 (-)
Sequence length	1833
RNA-Seq Expression	MC03g0139.1
Synteny	MC03g0139.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATGAGAAAAAAAGGAGACAAGAAATAGAAAGTGAGAAAGAAAAAAGAAACGCAGAGTTGGAAATAAAAATTCAACGAGAAATAGAAAGAAGGATAAAAAAAGAAATGAAAAGGGCAAAAAGAGTACTTACCATCTTCAAAACTCATAATTGCTTCGATCGAAGGAGAATAAAAAAGAAGAGAATAGAGAACACAGAGACAAAACGGTAACTTTCCATTCACCCGACCTCACAATTAGGTTACGAAAACTAATTTAAAGAAAAAATAGATTGTATTTCATTTAGCTCCACGTATTAAATTCACCCGCTAAAAAAGAAACAAGTTTAGGATTAAATTTAAAAATAAAATATAACCCAGCCGTCAAGTTTAGGATTTATCCAGCAGCCCAGCCCAGCCCAAACTTTGGTTTTGGCAGCCCCTTCTTGTTCCATCTGCAGGAGCAGCACGACGGCAGCCTCTCCACTCCACCGTCGCCTCCCCACTCTCTCTTTTTACACGATTTTCCGACGCCGCCGCCGCCTCTCCCTCCCTCTCTTGGTATCATCCATTCAACCTAATCAGGTAACTTTTCAACGATTGATGACGTTCACGCCCCCCCTCCTCTGTCTCTTGGCAAGCCCAATCAATCTCAAATCGGTGAAAATCCAAGTGACCCATTTGGTCTAAAACCCACTTTGTTTGGAATATCGGTTCGGTTGTTCAACCGAACCCATGTTCACTCCTCCCAGCGACTCACTTGAAATTTGCATTTGAGCAGCAGTTTTCCAATGGCAATTCGGGGGTTTTCGAGGTCGAAACTACCAATTTTTGCTTCGGTATTCCTTCGAGGTCTAACGAGGAGACCCATAAGAGATATTCCATTACCAGTGAAATCGAAGATTACCGGGTTTCAGCCGGATTCGAGCCGACCCATATGCGGATTCCGGCCTTATCACGACGGAAGGCCGCGGGGACCCCTTTGGAGAAGCCGGAAGGCAATTGGGAAAGAAGCGCTTTTCGTTATTCAGGGATTGAAGAGATTCAAGGAAGACGAAGAGAAGCTCCAGAAGTTCATGAAGAGTCATGTTTCGAGGCTCCTGAAATTGGATATGATTTCTGTGCTTGGTGAGCTTGAGCGGCAGGAAGAGGTTGCTTTAGCCGTCAAGGTTTCTTCCCTCTTTCCTTTTATTCAATATGATCTGTCCTTTTGTGGCTTTCATTATCTTAAGACTTTACTTCATGGGATGCCATAGAAATAGATAACGGGTTTCTGTAAAATTCCCATTAAAATCCCTGTTTTGCTCTTGATTGTTGTCTCAACTTTTCAAAAACCTTGTAAGTTGATGCTTTGAATTTTTGCTTCATAACTGTTCTGTTGCTACTTTTTAGTTACCTGGGGAGAATAACTTCGCAAATGCTGGATTTAAGCTTCTATTTCGCTCTTGGATTTCTTAGAATATGATTCTCAGCTTCTAGCATTCTTAGATATGAATATGATGTTATTGGATATTGTGTATTGTGCAATTTCTGCAAGTTGCAGCGCTTCACTTCTTTAGGAAGGTTGTAAACAAAATAAATTCATGTATTGATATGGTGAGTTGTTTTCCCACTTCCTGTTAAGTTTTTAGCTTATTTCATGTCGAATGTCATCTCATGCTTTCAAACTTCTTTCTTCCTTGGGAAAACTGTCTTGTCGCTCTCTTGTTCAACTCTAACCTGCCCCTTTGAGTGCTTTATCAGGTCGACTTCAAACCGTCTGTATGACTTAAGATCTTAATATGAAATCTAGTTCAATCCGGTTGTGTGTAGAAATATTTCCTGTTAGATTCTGTTAGTTCAATTGATTTATCTTCATTGTTTCTGGTAGTGGTAATCTGACCCGGTGCAGTTTTTCATTGTCATTATTTGCTCTACCTTACATCTTTGTTGGATTTACAGATATTTAGGTTGATTCAAAAGCAAGACTGGTACAAGCCCGATGTCTTTATGTATAAAGACTTGATCATCGCGTTAGCCGGAAGTAAAAAGATGGATGATGCAATGGAATTATGGGAGAGCATGAGGAAGGAGGATTTATTCCCCGACTCTCAAACGTATACCGAAGTCATCAGAGGTTTCTTGAGGTATGGATCGCCTTCTGATGCAATGAACATATATGAGGATATGAAGAAGTCTCCAGATCCACCAGATGAGTTGCCATTCAGAATTTTGCTGAAGGGACTTCTGCCACACCCCCTTTTGAGAAATAAAGTAAAGCAAGATTTTGAGGAGCTCTTTCCTGACCAGAATGTCTACGATCCCCCAGAAGAGATTTTCGGCCTACGTTGAGGTTTGTGGTTAACGTTGATTTTTTTGCTTGTACCATCATCTGAAGGTTGGTAGGACTTAGTAGTTAGTAGGCCAGTGTATTCATCAACTTAAGTAGTTATGAGATCAAAATCTTTGAGAATGTTTTACAAGTCTTTAACTACCAAATATTGTGGATCCATTTAGGTTGTAGTCCTAGCTCATTTTTTCTAGGTGCCACTCAAAGACTCCATCGAGATATTTTGGAAAAAAAAGAGAGAAAAAAAAGGAACAACTATTGAGATAAAGATGGGTCCTCAATAACATAGACGAGGTAAGATGAATTATTGGTCAGATCTAGTATGGGTCGATATGTGGATAGTAGTTGGAGCAAAGAACTCTCTTATGGTTTTCCCATCTAGGATCCCTAGGGAGAACGATCAGTTGGTCCATGTGATTACATGTAAACAACTAGATGCACTATCTCCTTTTAACCCATTTAGCAAAATGTAGTAAAAAAGAAGGAAAATCCAAAGATCGACCCGTGGTCTTTGGGGTCACGAACCAACCATAAAACTCTTCTCAACAAGTAGAATGCAGTAGTTGTACGCTTTCTTGTTGGGTTTGTAGAGTATTTTGGGAGAGTACACAATTGTTTTTCTTTTATATGAAAAACGGGGTTTATCTCCACTGTAGCGGCTTGGCCAGGAGGGTAAGAAGTTCCATAAATAGGTCTTTTTGAGGTTTGAAATATTTTGACTTTTAATTCAGTTTTGCTATCAAACAAAAGGTCCATGATATTTCCCATCATACAAATTTCCATCTTATCACTTACATGTGCTTTTTATTTTGGCCTCTAAAACATTTGAAACATTTTTCAGCTGAATAAGTATTTTGTGATTGCTTCATCTTTTTCTCCTTTCCGTCAACCTTACCAGGTTCTTTTAGGAAGATCTTTAGCTTCTTTACATCAGGTGCTTGTTCTTGTCGAACAACGATGCAAGAAAGACAATAATTAGAGGGAAGCAAATTCATCACATTGTAAAAATAACCAATTGGAAACCGAAACATCGAGCTTTCTTTTGTACGGGTCTGTTCGAGTACTGCATTAACTCTGAATCCATCCCAATATATTATGCTGTGTGTTTGTTCCCTTTTTCTTTACATTCTCTTGAAGAAGTCGCATTGTTTCAGGGAATTAACACATTCCTCAGTTTGTCGACGAACATTCCCCTCTGCTGAAAGCAAAACCAAGCAAGGGAAGATTGATGATATGAAAATGCAAGGAGATGGAGAAGTTATTAATATGGTGTTCCTCGAAGCGGTCGACTCAGATTTGGATTCAAATGAAGGTGTTGTCTTTTAGGGAAGTGAGTGTACCTTTGCCCAGAAATGTCTATTTTACCAATGAATATGAACACCACAGAGAGGTACAAATTGCCATTGATGTCTCCCTTAAGGGGGATTCTTCTTCTCCTTTTCTTAATATAGTTTTGTAACTTAATATCTTTGTTTAGAATTTCTTTTCTTAGCTTTTAAAATCTTAACTTTAATTTTTTTTTTCAGTTGCCTTAGCCAGTGAGCTATATTTTGATTGGTTTAATTTAAATGGTTCAATCTTCATGAATTTTAATTGTATATGACATATGTAGATAATGAATGGAGTTGTATTATATTTTTTTTATAAAG

mRNA sequence

GAATGAGAAAAAAAGGAGACAAGAAATAGAAAGTGAGAAAGAAAAAAGAAACGCAGAGTTGGAAATAAAAATTCAACGAGAAATAGAAAGAAGGATAAAAAAAGAAATGAAAAGGGCAAAAAGAGTACTTACCATCTTCAAAACTCATAATTGCTTCGATCGAAGGAGAATAAAAAAGAAGAGAATAGAGAACACAGAGACAAAACGGTAACTTTCCATTCACCCGACCTCACAATTAGGTTACGAAAACTAATTTAAAGAAAAAATAGATTGTATTTCATTTAGCTCCACGTATTAAATTCACCCGCTAAAAAAGAAACAAGTTTAGGATTAAATTTAAAAATAAAATATAACCCAGCCGTCAAGTTTAGGATTTATCCAGCAGCCCAGCCCAGCCCAAACTTTGGTTTTGGCAGCCCCTTCTTGTTCCATCTGCAGGAGCAGCACGACGGCAGCCTCTCCACTCCACCGTCGCCTCCCCACTCTCTCTTTTTACACGATTTTCCGACGCCGCCGCCGCCTCTCCCTCCCTCTCTTGGTATCATCCATTCAACCTAATCAGTTTTCCAATGGCAATTCGGGGGTTTTCGAGGTCGAAACTACCAATTTTTGCTTCGGTATTCCTTCGAGGTCTAACGAGGAGACCCATAAGAGATATTCCATTACCAGTGAAATCGAAGATTACCGGGTTTCAGCCGGATTCGAGCCGACCCATATGCGGATTCCGGCCTTATCACGACGGAAGGCCGCGGGGACCCCTTTGGAGAAGCCGGAAGGCAATTGGGAAAGAAGCGCTTTTCGTTATTCAGGGATTGAAGAGATTCAAGGAAGACGAAGAGAAGCTCCAGAAGTTCATGAAGAGTCATGTTTCGAGGCTCCTGAAATTGGATATGATTTCTGTGCTTGGTGAGCTTGAGCGGCAGGAAGAGGTTGCTTTAGCCGTCAAGATATTTAGGTTGATTCAAAAGCAAGACTGGTACAAGCCCGATGTCTTTATGTATAAAGACTTGATCATCGCGTTAGCCGGAAGTAAAAAGATGGATGATGCAATGGAATTATGGGAGAGCATGAGGAAGGAGGATTTATTCCCCGACTCTCAAACGTATACCGAAGTCATCAGAGGTTTCTTGAGGTATGGATCGCCTTCTGATGCAATGAACATATATGAGGATATGAAGAAGTCTCCAGATCCACCAGATGAGTTGCCATTCAGAATTTTGCTGAAGGGACTTCTGCCACACCCCCTTTTGAGAAATAAAGTAAAGCAAGATTTTGAGGAGCTCTTTCCTGACCAGAATGTCTACGATCCCCCAGAAGAGATTTTCGGCCTACGTTGAGGGAATTAACACATTCCTCAGTTTGTCGACGAACATTCCCCTCTGCTGAAAGCAAAACCAAGCAAGGGAAGATTGATGATATGAAAATGCAAGGAGATGGAGAAGTTATTAATATGGTGTTCCTCGAAGCGGTCGACTCAGATTTGGATTCAAATGAAGGTGTTGTCTTTTAGGGAAGTGAGTGTACCTTTGCCCAGAAATGTCTATTTTACCAATGAATATGAACACCACAGAGAGGTACAAATTGCCATTGATGTCTCCCTTAAGGGGGATTCTTCTTCTCCTTTTCTTAATATAGTTTTGTAACTTAATATCTTTGTTTAGAATTTCTTTTCTTAGCTTTTAAAATCTTAACTTTAATTTTTTTTTTCAGTTGCCTTAGCCAGTGAGCTATATTTTGATTGGTTTAATTTAAATGGTTCAATCTTCATGAATTTTAATTGTATATGACATATGTAGATAATGAATGGAGTTGTATTATATTTTTTTTATAAAG

Coding sequence (CDS)

ATGGCAATTCGGGGGTTTTCGAGGTCGAAACTACCAATTTTTGCTTCGGTATTCCTTCGAGGTCTAACGAGGAGACCCATAAGAGATATTCCATTACCAGTGAAATCGAAGATTACCGGGTTTCAGCCGGATTCGAGCCGACCCATATGCGGATTCCGGCCTTATCACGACGGAAGGCCGCGGGGACCCCTTTGGAGAAGCCGGAAGGCAATTGGGAAAGAAGCGCTTTTCGTTATTCAGGGATTGAAGAGATTCAAGGAAGACGAAGAGAAGCTCCAGAAGTTCATGAAGAGTCATGTTTCGAGGCTCCTGAAATTGGATATGATTTCTGTGCTTGGTGAGCTTGAGCGGCAGGAAGAGGTTGCTTTAGCCGTCAAGATATTTAGGTTGATTCAAAAGCAAGACTGGTACAAGCCCGATGTCTTTATGTATAAAGACTTGATCATCGCGTTAGCCGGAAGTAAAAAGATGGATGATGCAATGGAATTATGGGAGAGCATGAGGAAGGAGGATTTATTCCCCGACTCTCAAACGTATACCGAAGTCATCAGAGGTTTCTTGAGGTATGGATCGCCTTCTGATGCAATGAACATATATGAGGATATGAAGAAGTCTCCAGATCCACCAGATGAGTTGCCATTCAGAATTTTGCTGAAGGGACTTCTGCCACACCCCCTTTTGAGAAATAAAGTAAAGCAAGATTTTGAGGAGCTCTTTCCTGACCAGAATGTCTACGATCCCCCAGAAGAGATTTTCGGCCTACGTTGA

Protein sequence

MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRPRGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEEVALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYTEVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFPDQNVYDPPEEIFGLR

Homology

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BLAST of MC03g0139.1 vs. ExPASy Swiss-Prot
Match: Q9STF9 (Protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THA8L PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 2.9e-97
Identity = 177/254 (69.69%), Postives = 208/254 (81.89%), Query Frame = 0


Query: 2   AIRGFSRSKLPIFASVFLRGLTRRP-IRDIPLPVKSKITGFQPDSSRPICGF-RPYHDGR 61
           AIR  SR K P FAS+F + +TR P I  I        T   P   +P   F   +HDGR
Sbjct: 3   AIRVCSR-KFPTFASIFFQNITRNPSIHRISFSNLKPKTLLHPIPPKPFTVFVSRFHDGR 62

Query: 62  PRGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQE 121
           PRGPLWR +K IGKEALFVI GLKR KED+EKL KF+K+HV RLLKLDM++V+GELERQE
Sbjct: 63  PRGPLWRGKKLIGKEALFVILGLKRLKEDDEKLDKFIKTHVFRLLKLDMLAVIGELERQE 122

Query: 122 EVALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTY 181
           E ALA+K+F +IQKQ+WY+PDVFMYKDLI++LA SK+MD+AM LWE M+KE+LFPDSQTY
Sbjct: 123 ETALAIKMFEVIQKQEWYQPDVFMYKDLIVSLAKSKRMDEAMALWEKMKKENLFPDSQTY 182

Query: 182 TEVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELF 241
           TEVIRGFLR G P+DAMN+YEDM KSPDPP+ELPFR+LLKGLLPHPLLRNKVK+DFEELF
Sbjct: 183 TEVIRGFLRDGCPADAMNVYEDMLKSPDPPEELPFRVLLKGLLPHPLLRNKVKKDFEELF 242

Query: 242 PDQNVYDPPEEIFG 254
           P+++ YDPPEEIFG
Sbjct: 243 PEKHAYDPPEEIFG 255

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BLAST of MC03g0139.1 vs. ExPASy Swiss-Prot
Match: Q1PFH7 (Pentatricopeptide repeat-containing protein At1g62350 OS=Arabidopsis thaliana OX=3702 GN=At1g62350 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.1e-47
Identity = 85/181 (46.96%), Postives = 132/181 (72.93%), Query Frame = 0


Query: 71  IGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEEVALAVKIFRL 130
           + KE L   + LKR +    +L +F+ SHVSRLLK D++SVL E +RQ +V L +K++ +
Sbjct: 1   MSKEGLIAAKELKRLQTQSVRLDRFIGSHVSRLLKSDLVSVLAEFQRQNQVFLCMKLYEV 60

Query: 131 IQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYTEVIRGFLRYG 190
           ++++ WY+PD+F Y+D+++ LA +KK+D+  ++WE ++KE++  D  T+ +++RGFL   
Sbjct: 61  VRREIWYRPDMFFYRDMLMMLARNKKVDETKKVWEDLKKEEVLFDQHTFGDLVRGFLDNE 120

Query: 191 SPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFPDQNVYDPPEE 250
            P +AM +Y +M++SPD P  LPFR++LKGL+P+P LR KVK DF ELFP   VYDPPE+
Sbjct: 121 LPLEAMRLYGEMRESPDRPLSLPFRVILKGLVPYPELREKVKDDFLELFPGMIVYDPPED 180

Query: 251 I 252
           I
Sbjct: 181 I 181

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BLAST of MC03g0139.1 vs. ExPASy Swiss-Prot
Match: Q9FKC3 (Pentatricopeptide repeat-containing protein At5g48730, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g48730 PE=2 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 8.6e-09
Identity = 45/158 (28.48%), Postives = 77/158 (48.73%), Query Frame = 0


Query: 66  RSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEEVALAV 125
           ++ K   ++A+ +I   +  K   EK     K    +LL   ++  L E         A+
Sbjct: 80  KNTKIASRKAISIILRREATKSIIEK-----KKGSKKLLPRTVLESLHERITALRWESAI 139

Query: 126 KIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYTEVIRG 185
           ++F L+++Q WYKP+V +Y  LI+ L   K+ + A EL++ M  E    + + YT ++  
Sbjct: 140 QVFELLREQLWYKPNVGIYVKLIVMLGKCKQPEKAHELFQEMINEGCVVNHEVYTALVSA 199

Query: 186 FLRYGSPSDAMNIYEDMKKSPD-PPDELPFRILLKGLL 223
           + R G    A  + E MK S +  PD   + IL+K  L
Sbjct: 200 YSRSGRFDAAFTLLERMKSSHNCQPDVHTYSILIKSFL 232

[Close]

BLAST of MC03g0139.1 vs. ExPASy Swiss-Prot
Match: Q9FMQ1 (Pentatricopeptide repeat-containing protein At5g12100, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g12100 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.9e-08
Identity = 35/102 (34.31%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 120 EVALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTY 179
           +V   +++F  + K D   P VF+Y  LI  L   K+M+DA +L++ M    L P   TY
Sbjct: 194 DVGKGLELFNRM-KHDRIYPSVFIYNVLIDGLCKGKRMNDAEQLFDEMLARRLLPSLITY 253

Query: 180 TEVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGL 222
             +I G+ + G+P  +  + E MK     P  + F  LLKGL
Sbjct: 254 NTLIDGYCKAGNPEKSFKVRERMKADHIEPSLITFNTLLKGL 294

[Close]

BLAST of MC03g0139.1 vs. ExPASy Swiss-Prot
Match: Q9C8T7 (Pentatricopeptide repeat-containing protein At1g63330 OS=Arabidopsis thaliana OX=3702 GN=At1g63330 PE=2 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-07
Identity = 32/98 (32.65%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 124 AVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYTEVI 183
           A ++F  +  +D + PD+  Y  LI     SK+++D  EL+  M    L  D+ TYT +I
Sbjct: 309 AKQMFEFMVSKDCF-PDLDTYNTLIKGFCKSKRVEDGTELFREMSHRGLVGDTVTYTTLI 368

Query: 184 RGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGL 222
           +G    G   +A  +++ M     PPD + + ILL GL
Sbjct: 369 QGLFHDGDCDNAQKVFKQMVSDGVPPDIMTYSILLDGL 405

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BLAST of MC03g0139.1 vs. NCBI nr
Match: XP_022158131.1 (pentatricopeptide repeat-containing protein At3g46870 [Momordica charantia])

HSP 1 Score: 514 bits (1325), Expect = 4.63e-184
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP
Sbjct: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT
Sbjct: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQNVYDPPEEIFGLR
Sbjct: 241 DQNVYDPPEEIFGLR 255

[Close]

BLAST of MC03g0139.1 vs. NCBI nr
Match: XP_038895593.1 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida] >XP_038895594.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida] >XP_038895595.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida])

HSP 1 Score: 479 bits (1232), Expect = 6.97e-170
Identity = 239/255 (93.73%), Postives = 247/255 (96.86%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MAIR FSRSKLPIFASVFLRGLTRRPIRD  LPVKS ITGFQPDSSRP+ GFR YHDGRP
Sbjct: 1   MAIRWFSRSKLPIFASVFLRGLTRRPIRDSSLPVKSTITGFQPDSSRPLWGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL+KFMKSHV RLLKLDMISVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMISVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVKIFRLIQKQDWYKPDVF+YKDLIIALA SKKMDDAM+LWESMR+E+LFPDSQTYT
Sbjct: 121 VALAVKIFRLIQKQDWYKPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRN+VKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+VYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

[Close]

BLAST of MC03g0139.1 vs. NCBI nr
Match: XP_008457435.1 (PREDICTED: pentatricopeptide repeat-containing protein At3g46870 [Cucumis melo] >KAA0031765.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 470 bits (1210), Expect = 1.57e-166
Identity = 232/255 (90.98%), Postives = 246/255 (96.47%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MAIR FSRSKLPIFA VFLRGLTRRPIRD+PLPV S ITGFQPDSSRPI GFR YHDGRP
Sbjct: 1   MAIRWFSRSKLPIFALVFLRGLTRRPIRDVPLPVTSTITGFQPDSSRPIWGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL KFMKSHVSRLLKLDM++VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLGKFMKSHVSRLLKLDMLAVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVKIFRLI+KQDWYKPDV++YKDLIIALA SKKMDDAM+LWESMR+E+LFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFLRYGSPSDAMN+YEDMKKSP+PPDELPFRILLKGLLPHPLLRN+VKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPEPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+V+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

[Close]

BLAST of MC03g0139.1 vs. NCBI nr
Match: XP_022964699.1 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >XP_022964700.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >XP_022964701.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >KAG7019169.1 Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466 bits (1198), Expect = 1.06e-164
Identity = 228/255 (89.41%), Postives = 243/255 (95.29%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MA+R FS+ KLPI  SVFLRGL RRPIRDIPLPVKS + GFQPDSSRP+ GFR YHDGRP
Sbjct: 1   MAMRWFSKLKLPICYSVFLRGLMRRPIRDIPLPVKSTVAGFQPDSSRPLSGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMK HV RLLKLDMI+VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKCHVLRLLKLDMIAVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVK+FRLIQKQ+WYKPDVF+YKDLI+ALA SK+MDDAMELWESMRKE+LFPDSQTYT
Sbjct: 121 VALAVKVFRLIQKQEWYKPDVFLYKDLIVALARSKQMDDAMELWESMRKENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFL+YGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRN+VKQDFEE+FP
Sbjct: 181 EVIRGFLKYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEEIFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+VYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

[Close]

BLAST of MC03g0139.1 vs. NCBI nr
Match: XP_022970562.1 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita maxima] >XP_022970563.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita maxima] >XP_022970564.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita maxima])

HSP 1 Score: 464 bits (1195), Expect = 3.04e-164
Identity = 230/255 (90.20%), Postives = 243/255 (95.29%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MA+R FS+SKLPI  SVFLRGLTRRPIRDIPLPVKS I GF PDSSR + GFR YHDGRP
Sbjct: 1   MAMRWFSKSKLPICYSVFLRGLTRRPIRDIPLPVKSTIAGFLPDSSRLLSGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMK HV RLLKLDMI+VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKCHVLRLLKLDMIAVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVK+FRLIQKQ+W+KPDVF+YKDLI+ALA SKKMDDAMELWESMRKE+LFPDSQTYT
Sbjct: 121 VALAVKVFRLIQKQEWFKPDVFIYKDLIVALARSKKMDDAMELWESMRKENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFL+YGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRN+VKQDFEELFP
Sbjct: 181 EVIRGFLKYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+VYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

[Close]

BLAST of MC03g0139.1 vs. ExPASy TrEMBL
Match: A0A6J1E045 (pentatricopeptide repeat-containing protein At3g46870 OS=Momordica charantia OX=3673 GN=LOC111024687 PE=4 SV=1)

HSP 1 Score: 514 bits (1325), Expect = 2.24e-184
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP
Sbjct: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT
Sbjct: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQNVYDPPEEIFGLR
Sbjct: 241 DQNVYDPPEEIFGLR 255

[Close]

BLAST of MC03g0139.1 vs. ExPASy TrEMBL
Match: A0A5A7SRJ7 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00030 PE=4 SV=1)

HSP 1 Score: 470 bits (1210), Expect = 7.62e-167
Identity = 232/255 (90.98%), Postives = 246/255 (96.47%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MAIR FSRSKLPIFA VFLRGLTRRPIRD+PLPV S ITGFQPDSSRPI GFR YHDGRP
Sbjct: 1   MAIRWFSRSKLPIFALVFLRGLTRRPIRDVPLPVTSTITGFQPDSSRPIWGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL KFMKSHVSRLLKLDM++VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLGKFMKSHVSRLLKLDMLAVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVKIFRLI+KQDWYKPDV++YKDLIIALA SKKMDDAM+LWESMR+E+LFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFLRYGSPSDAMN+YEDMKKSP+PPDELPFRILLKGLLPHPLLRN+VKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPEPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+V+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

[Close]

BLAST of MC03g0139.1 vs. ExPASy TrEMBL
Match: A0A0A0LVE9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525290 PE=4 SV=1)

HSP 1 Score: 470 bits (1210), Expect = 7.62e-167
Identity = 230/255 (90.20%), Postives = 246/255 (96.47%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MAIR FSRSKLP+FASVFL+GLTRRPIRD+PLPVKS IT FQPDS RPI GFR YHDGRP
Sbjct: 1   MAIRWFSRSKLPVFASVFLQGLTRRPIRDVPLPVKSTITDFQPDSRRPIWGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEK +KFMKSHVSRLLKLDM++VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKFEKFMKSHVSRLLKLDMVAVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVKIFRLI+KQDWYKPDV++YKDLIIALA SKKMDDAM+LWESMR+E+LFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFLRYGSPSDAMN+YEDMKKSPDPPDELPFRILLKGLLPHPLLRN+VKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+V+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

[Close]

BLAST of MC03g0139.1 vs. ExPASy TrEMBL
Match: A0A1S3C5H5 (pentatricopeptide repeat-containing protein At3g46870 OS=Cucumis melo OX=3656 GN=LOC103497124 PE=4 SV=1)

HSP 1 Score: 470 bits (1210), Expect = 7.62e-167
Identity = 232/255 (90.98%), Postives = 246/255 (96.47%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MAIR FSRSKLPIFA VFLRGLTRRPIRD+PLPV S ITGFQPDSSRPI GFR YHDGRP
Sbjct: 1   MAIRWFSRSKLPIFALVFLRGLTRRPIRDVPLPVTSTITGFQPDSSRPIWGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL KFMKSHVSRLLKLDM++VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLGKFMKSHVSRLLKLDMLAVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVKIFRLI+KQDWYKPDV++YKDLIIALA SKKMDDAM+LWESMR+E+LFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFLRYGSPSDAMN+YEDMKKSP+PPDELPFRILLKGLLPHPLLRN+VKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPEPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+V+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

[Close]

BLAST of MC03g0139.1 vs. ExPASy TrEMBL
Match: A0A6J1HLK1 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464704 PE=4 SV=1)

HSP 1 Score: 466 bits (1198), Expect = 5.14e-165
Identity = 228/255 (89.41%), Postives = 243/255 (95.29%), Query Frame = 0


Query: 1   MAIRGFSRSKLPIFASVFLRGLTRRPIRDIPLPVKSKITGFQPDSSRPICGFRPYHDGRP 60
           MA+R FS+ KLPI  SVFLRGL RRPIRDIPLPVKS + GFQPDSSRP+ GFR YHDGRP
Sbjct: 1   MAMRWFSKLKLPICYSVFLRGLMRRPIRDIPLPVKSTVAGFQPDSSRPLSGFRLYHDGRP 60

Query: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEE 120
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMK HV RLLKLDMI+VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKCHVLRLLKLDMIAVLGELERQEE 120

Query: 121 VALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYT 180
           VALAVK+FRLIQKQ+WYKPDVF+YKDLI+ALA SK+MDDAMELWESMRKE+LFPDSQTYT
Sbjct: 121 VALAVKVFRLIQKQEWYKPDVFLYKDLIVALARSKQMDDAMELWESMRKENLFPDSQTYT 180

Query: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFP 240
           EVIRGFL+YGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRN+VKQDFEE+FP
Sbjct: 181 EVIRGFLKYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEEIFP 240

Query: 241 DQNVYDPPEEIFGLR 255
           DQ+VYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

[Close]

BLAST of MC03g0139.1 vs. TAIR 10
Match: AT3G46870.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 2.1e-98
Identity = 177/254 (69.69%), Postives = 208/254 (81.89%), Query Frame = 0


Query: 2   AIRGFSRSKLPIFASVFLRGLTRRP-IRDIPLPVKSKITGFQPDSSRPICGF-RPYHDGR 61
           AIR  SR K P FAS+F + +TR P I  I        T   P   +P   F   +HDGR
Sbjct: 3   AIRVCSR-KFPTFASIFFQNITRNPSIHRISFSNLKPKTLLHPIPPKPFTVFVSRFHDGR 62

Query: 62  PRGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQE 121
           PRGPLWR +K IGKEALFVI GLKR KED+EKL KF+K+HV RLLKLDM++V+GELERQE
Sbjct: 63  PRGPLWRGKKLIGKEALFVILGLKRLKEDDEKLDKFIKTHVFRLLKLDMLAVIGELERQE 122

Query: 122 EVALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTY 181
           E ALA+K+F +IQKQ+WY+PDVFMYKDLI++LA SK+MD+AM LWE M+KE+LFPDSQTY
Sbjct: 123 ETALAIKMFEVIQKQEWYQPDVFMYKDLIVSLAKSKRMDEAMALWEKMKKENLFPDSQTY 182

Query: 182 TEVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELF 241
           TEVIRGFLR G P+DAMN+YEDM KSPDPP+ELPFR+LLKGLLPHPLLRNKVK+DFEELF
Sbjct: 183 TEVIRGFLRDGCPADAMNVYEDMLKSPDPPEELPFRVLLKGLLPHPLLRNKVKKDFEELF 242

Query: 242 PDQNVYDPPEEIFG 254
           P+++ YDPPEEIFG
Sbjct: 243 PEKHAYDPPEEIFG 255

[Close]

BLAST of MC03g0139.1 vs. TAIR 10
Match: AT1G62350.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 1.5e-48
Identity = 85/181 (46.96%), Postives = 132/181 (72.93%), Query Frame = 0


Query: 71  IGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEEVALAVKIFRL 130
           + KE L   + LKR +    +L +F+ SHVSRLLK D++SVL E +RQ +V L +K++ +
Sbjct: 1   MSKEGLIAAKELKRLQTQSVRLDRFIGSHVSRLLKSDLVSVLAEFQRQNQVFLCMKLYEV 60

Query: 131 IQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYTEVIRGFLRYG 190
           ++++ WY+PD+F Y+D+++ LA +KK+D+  ++WE ++KE++  D  T+ +++RGFL   
Sbjct: 61  VRREIWYRPDMFFYRDMLMMLARNKKVDETKKVWEDLKKEEVLFDQHTFGDLVRGFLDNE 120

Query: 191 SPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNKVKQDFEELFPDQNVYDPPEE 250
            P +AM +Y +M++SPD P  LPFR++LKGL+P+P LR KVK DF ELFP   VYDPPE+
Sbjct: 121 LPLEAMRLYGEMRESPDRPLSLPFRVILKGLVPYPELREKVKDDFLELFPGMIVYDPPED 180

Query: 251 I 252
           I
Sbjct: 181 I 181

[Close]

BLAST of MC03g0139.1 vs. TAIR 10
Match: AT5G09320.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 83.6 bits (205), Expect = 2.6e-16
Identity = 58/177 (32.77%), Postives = 88/177 (49.72%), Query Frame = 0


Query: 61  RGPLWRSRKAIGKEALFVIQGLKR---------------FKEDEEKLQKFMKSHVSRLLK 120
           R PL R R  +  EA+  +Q LKR                      L + + S   RLLK
Sbjct: 496 RKPLQRGR-MLSIEAIQAVQALKRANPLLPPPPVPSTSTTSSSSALLDRVIISKFRRLLK 555

Query: 121 LDMISVLGELERQEEVALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWE 180
            DM++VL EL RQ E +LA+K+F  I+K+ WYKP V MY D+I  +A +  M++   L+ 
Sbjct: 556 FDMVAVLRELLRQNECSLALKVFEEIRKEYWYKPQVRMYTDMITVMADNSLMEEVNYLYS 615

Query: 181 SMRKE-DLFPDSQTYTEVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGL 222
           +M+ E  L  + + +  ++   L +      M+ Y  M+     PD   FR+L+ GL
Sbjct: 616 AMKSEKGLMAEIEWFNTLLTILLNHKLFDLVMDCYAFMQSIGYEPDRASFRVLVLGL 671

[Close]

BLAST of MC03g0139.1 vs. TAIR 10
Match: AT5G48730.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 6.1e-10
Identity = 45/158 (28.48%), Postives = 77/158 (48.73%), Query Frame = 0


Query: 66  RSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKSHVSRLLKLDMISVLGELERQEEVALAV 125
           ++ K   ++A+ +I   +  K   EK     K    +LL   ++  L E         A+
Sbjct: 80  KNTKIASRKAISIILRREATKSIIEK-----KKGSKKLLPRTVLESLHERITALRWESAI 139

Query: 126 KIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTYTEVIRG 185
           ++F L+++Q WYKP+V +Y  LI+ L   K+ + A EL++ M  E    + + YT ++  
Sbjct: 140 QVFELLREQLWYKPNVGIYVKLIVMLGKCKQPEKAHELFQEMINEGCVVNHEVYTALVSA 199

Query: 186 FLRYGSPSDAMNIYEDMKKSPD-PPDELPFRILLKGLL 223
           + R G    A  + E MK S +  PD   + IL+K  L
Sbjct: 200 YSRSGRFDAAFTLLERMKSSHNCQPDVHTYSILIKSFL 232

[Close]

BLAST of MC03g0139.1 vs. TAIR 10
Match: AT5G12100.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 61.2 bits (147), Expect = 1.4e-09
Identity = 35/102 (34.31%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 120 EVALAVKIFRLIQKQDWYKPDVFMYKDLIIALAGSKKMDDAMELWESMRKEDLFPDSQTY 179
           +V   +++F  + K D   P VF+Y  LI  L   K+M+DA +L++ M    L P   TY
Sbjct: 194 DVGKGLELFNRM-KHDRIYPSVFIYNVLIDGLCKGKRMNDAEQLFDEMLARRLLPSLITY 253

Query: 180 TEVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGL 222
             +I G+ + G+P  +  + E MK     P  + F  LLKGL
Sbjct: 254 NTLIDGYCKAGNPEKSFKVRERMKADHIEPSLITFNTLLKGL 294

The following BLAST results are available for this feature:

BLAST of MC03g0139.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9STF9	2.9e-97	69.69	Protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q1PFH7	2.1e-47	46.96	Pentatricopeptide repeat-containing protein At1g62350 OS=Arabidopsis thaliana OX...	[more]
Q9FKC3	8.6e-09	28.48	Pentatricopeptide repeat-containing protein At5g48730, chloroplastic OS=Arabidop...	[more]
Q9FMQ1	1.9e-08	34.31	Pentatricopeptide repeat-containing protein At5g12100, mitochondrial OS=Arabidop...	[more]
Q9C8T7	1.2e-07	32.65	Pentatricopeptide repeat-containing protein At1g63330 OS=Arabidopsis thaliana OX...	[more]

BLAST of MC03g0139.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158131.1	4.63e-184	100.00	pentatricopeptide repeat-containing protein At3g46870 [Momordica charantia]	[more]
XP_038895593.1	6.97e-170	93.73	protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida] >XP_0388955...	[more]
XP_008457435.1	1.57e-166	90.98	PREDICTED: pentatricopeptide repeat-containing protein At3g46870 [Cucumis melo] ...	[more]
XP_022964699.1	1.06e-164	89.41	protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >XP_022964...	[more]
XP_022970562.1	3.04e-164	90.20	protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita maxima] >XP_02297056...	[more]

BLAST of MC03g0139.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E045	2.24e-184	100.00	pentatricopeptide repeat-containing protein At3g46870 OS=Momordica charantia OX=...	[more]
A0A5A7SRJ7	7.62e-167	90.98	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0LVE9	7.62e-167	90.20	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525290 PE=4 SV=1	[more]
A0A1S3C5H5	7.62e-167	90.98	pentatricopeptide repeat-containing protein At3g46870 OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1HLK1	5.14e-165	89.41	protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of MC03g0139.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46870.1	2.1e-98	69.69	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT1G62350.1	1.5e-48	46.96	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT5G09320.1	2.6e-16	32.77	Vacuolar sorting protein 9 (VPS9) domain	[more]
AT5G48730.1	6.1e-10	28.48	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT5G12100.1	1.4e-09	34.31	pentatricopeptide (PPR) repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 69..255 e-value: 2.5E-55 score: 188.7
IPR002885	Pentatricopeptide repeat	PFAM	PF13041	PPR_2	coord: 139..187 e-value: 9.4E-9 score: 35.4
IPR002885	Pentatricopeptide repeat	TIGRFAM	TIGR00756	TIGR00756	coord: 178..205 e-value: 1.7E-4 score: 19.6 coord: 143..175 e-value: 6.2E-5 score: 20.9
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 175..209 score: 10.544828
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 140..174 score: 10.325603
None	No IPR available	PANTHER	PTHR46870:SF2	PROTEIN THYLAKOID ASSEMBLY 8-LIKE, CHLOROPLASTIC	coord: 4..254
IPR044795	Pentatricopeptide repeat-containing protein THA8L-like	PANTHER	PTHR46870	PROTEIN THYLAKOID ASSEMBLY 8-LIKE, CHLOROPLASTIC	coord: 4..254

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC03g0139	MC03g0139	gene

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC03g0139.1-utr3	MC03g0139.1-utr3-MC03:2383903..2384397	utr3
MC03g0139.1-utr3	MC03g0139.1-utr3-MC03:2385549..2385549	utr3

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC03g0139.1-cds	MC03g0139.1-cds-MC03:2385550..2385939	CDS
MC03g0139.1-cds	MC03g0139.1-cds-MC03:2386712..2387089	CDS

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC03g0139.1-utr5	MC03g0139.1-utr5-MC03:2387090..2387096	utr5
MC03g0139.1-utr5	MC03g0139.1-utr5-MC03:2387296..2387857	utr5

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC03g0139.1	MC03g0139.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005515	protein binding