MC02g0830.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0830.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NADH-ubiquinone oxidoreductase chain 4
Location	MC02: 6597275 .. 6597450 (+)
Sequence length	174
RNA-Seq Expression	MC02g0830.1
Synteny	MC02g0830.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAGAAAGATCAAAACAGCATATCATTTTTTCCTTTATACTTTACTGGGATCCGTTTTATGCTATTAGCTATTCTGTTGATTTTTCTCCAAAGAGGAACCACCAATTTACAAATATCATTAACCACAGAATTTAGTGAGCGGCGCCAAACCTTTCTATGGATTGCTTCTTTCGCC

mRNA sequence

CAAAGAAAGATCAAAACAGCATATCATTTTTTCCTTTATACTTTACTGGGATCCTTTATGCTATTAGCTATTCTGTTGATTTTTCTCCAAAGAGGAACCACCAATTTACAAATATCATTAACCACAGAATTTAGTGAGCGGCGCCAAACCTTTCTATGGATTGCTTCTTTCGCC

Coding sequence (CDS)

CAAAGAAAGATCAAAACAGCATATCATTTTTTCCTTTATACTTTACTGGGATCCTTTATGCTATTAGCTATTCTGTTGATTTTTCTCCAAAGAGGAACCACCAATTTACAAATATCATTAACCACAGAATTTAGTGAGCGGCGCCAAACCTTTCTATGGATTGCTTCTTTCGCC

Protein sequence

QRKIKTAYHFFLYTLLGSFMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA

Homology

[Close]

BLAST of MC02g0830.1 vs. ExPASy Swiss-Prot
Match: Q04050 (NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 6.7e-18
Identity = 51/59 (86.44%), Postives = 52/59 (88.14%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 59
           QRKIK AY FFLYTLLGS FMLLAILLI  Q GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 164 QRKIKAAYQFFLYTLLGSLFMLLAILLILFQTGTTDLQISLTTEFSERRQIFLWIASFA 222

[Close]

BLAST of MC02g0830.1 vs. ExPASy Swiss-Prot
Match: P93313 (NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 1.7e-16
Identity = 49/59 (83.05%), Postives = 50/59 (84.75%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 59
           QRKIK AY FFLYTLLGS FMLLAILLI  Q GTT+LQI LTTEFSERRQ FLWIA FA
Sbjct: 164 QRKIKAAYQFFLYTLLGSLFMLLAILLILFQTGTTDLQILLTTEFSERRQIFLWIAFFA 222

[Close]

BLAST of MC02g0830.1 vs. ExPASy Swiss-Prot
Match: P27572 (NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 2.8e-16
Identity = 49/59 (83.05%), Postives = 50/59 (84.75%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 59
           QRKIK AY FFLYTLLGS FMLLAILLI LQ GTT+LQI LTTEFSERRQ  LWIA FA
Sbjct: 164 QRKIKAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQILLTTEFSERRQILLWIAFFA 222

[Close]

BLAST of MC02g0830.1 vs. ExPASy Swiss-Prot
Match: P26848 (NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 PE=3 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 8.2e-16
Identity = 47/59 (79.66%), Postives = 49/59 (83.05%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 59
           QRKIK AY FFLYTL+GS FMLLAIL IF Q GTT+LQI LTTEFSERRQ  LWIA FA
Sbjct: 164 QRKIKAAYQFFLYTLMGSLFMLLAILFIFFQTGTTDLQILLTTEFSERRQILLWIAFFA 222

[Close]

BLAST of MC02g0830.1 vs. ExPASy Swiss-Prot
Match: P48915 (NADH-ubiquinone oxidoreductase chain 4 OS=Chondrus crispus OX=2769 GN=ND4 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 8.8e-10
Identity = 36/59 (61.02%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 59
           QRKI+ AY FFLYTL+GS  MLLA+L I+ Q GTT+LQI    +FSE  Q FLW++ FA
Sbjct: 161 QRKIRAAYQFFLYTLIGSLLMLLALLNIYFQTGTTDLQILWYIQFSEISQIFLWLSFFA 219

[Close]

BLAST of MC02g0830.1 vs. NCBI nr
Match: KAG6621657.1 (hypothetical protein I3842_Q007400, partial [Carya illinoinensis])

HSP 1 Score: 91.3 bits (225), Expect = 4.13e-21
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 1  QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
          QRKIK AY FFLYTLLGS FMLLAILLI LQ GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 6  QRKIKAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSERRQIFLWIASFA 64

[Close]

BLAST of MC02g0830.1 vs. NCBI nr
Match: WP_131796663.1 (hypothetical protein [Candidatus Frankia datiscae])

HSP 1 Score: 91.3 bits (225), Expect = 6.94e-21
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 1  QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
          QRKIK AY FFLYTLLGS FMLLAILLI LQ GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 30 QRKIKAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSERRQIFLWIASFA 88

[Close]

BLAST of MC02g0830.1 vs. NCBI nr
Match: TXG46259.1 (hypothetical protein EZV62_028290 [Acer yangbiense])

HSP 1 Score: 91.3 bits (225), Expect = 9.75e-21
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
           QRKIK AY FFLYTLLGS FMLLAILLI LQ GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 47  QRKIKAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSERRQIFLWIASFA 105

[Close]

BLAST of MC02g0830.1 vs. NCBI nr
Match: TIV33366.1 (hypothetical protein E5V91_33190, partial [Mesorhizobium sp.])

HSP 1 Score: 87.8 bits (216), Expect = 1.15e-20
Identity = 50/59 (84.75%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 1  QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
          QRKIK AY FFLYTLLGS FMLLAILLI  Q GT +LQISLTTEFSERRQ FLWIASFA
Sbjct: 30 QRKIKAAYQFFLYTLLGSVFMLLAILLILFQTGTADLQISLTTEFSERRQIFLWIASFA 88

[Close]

BLAST of MC02g0830.1 vs. NCBI nr
Match: KAB5511270.1 (hypothetical protein DKX38_030065 [Salix brachista])

HSP 1 Score: 91.3 bits (225), Expect = 2.34e-20
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
           QRKIK AY FFLYTLLGS FMLLAILLI LQ GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 98  QRKIKAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSERRQIFLWIASFA 156

[Close]

BLAST of MC02g0830.1 vs. ExPASy TrEMBL
Match: A0A5C7GP56 (Proton_antipo_M domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_028290 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 4.72e-21
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
           QRKIK AY FFLYTLLGS FMLLAILLI LQ GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 47  QRKIKAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSERRQIFLWIASFA 105

[Close]

BLAST of MC02g0830.1 vs. ExPASy TrEMBL
Match: A0A529UU08 (Proton_antipo_M domain-containing protein (Fragment) OS=Mesorhizobium sp. OX=1871066 GN=E5V91_33190 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 5.58e-21
Identity = 50/59 (84.75%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 1  QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
          QRKIK AY FFLYTLLGS FMLLAILLI  Q GT +LQISLTTEFSERRQ FLWIASFA
Sbjct: 30 QRKIKAAYQFFLYTLLGSVFMLLAILLILFQTGTADLQISLTTEFSERRQIFLWIASFA 88

[Close]

BLAST of MC02g0830.1 vs. ExPASy TrEMBL
Match: A0A5N5IZ13 (NADH-ubiquinone oxidoreductase chain 4 OS=Salix brachista OX=2182728 GN=DKX38_030065 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.13e-20
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
           QRKIK AY FFLYTLLGS FMLLAILLI LQ GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 98  QRKIKAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSERRQIFLWIASFA 156

[Close]

BLAST of MC02g0830.1 vs. ExPASy TrEMBL
Match: A0A223GFW0 (NADH-ubiquinone oxidoreductase chain 4 (Fragment) OS=Sisyrinchium pachyrhizum subsp. pachyrhizum OX=2020648 GN=nad4 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.31e-20
Identity = 50/59 (84.75%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 1  QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
          QRKIK AY FFLYTLLGS FMLLAI LI LQ GTT+LQI LTTEFSERRQ FLWIASFA
Sbjct: 10 QRKIKAAYQFFLYTLLGSVFMLLAIPLILLQTGTTDLQILLTTEFSERRQIFLWIASFA 68

[Close]

BLAST of MC02g0830.1 vs. ExPASy TrEMBL
Match: A0A223GFV9 (NADH-ubiquinone oxidoreductase chain 4 (Fragment) OS=Sisyrinchium marginatum OX=2020647 GN=nad4 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.31e-20
Identity = 50/59 (84.75%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 1  QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 58
          QRKIK AY FFLYTLLGS FMLLAI LI LQ GTT+LQI LTTEFSERRQ FLWIASFA
Sbjct: 10 QRKIKAAYQFFLYTLLGSVFMLLAIPLILLQTGTTDLQILLTTEFSERRQIFLWIASFA 68

[Close]

BLAST of MC02g0830.1 vs. TAIR 10
Match: ATMG00580.1 (NADH dehydrogenase subunit 4 )

HSP 1 Score: 90.5 bits (223), Expect = 4.8e-19
Identity = 51/59 (86.44%), Postives = 52/59 (88.14%), Query Frame = 0


Query: 1   QRKIKTAYHFFLYTLLGS-FMLLAILLIFLQRGTTNLQISLTTEFSERRQTFLWIASFA 59
           QRKIK AY FFLYTLLGS FMLLAILLI  Q GTT+LQISLTTEFSERRQ FLWIASFA
Sbjct: 164 QRKIKAAYQFFLYTLLGSLFMLLAILLILFQTGTTDLQISLTTEFSERRQIFLWIASFA 222

The following BLAST results are available for this feature:

BLAST of MC02g0830.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q04050	6.7e-18	86.44	NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=...	[more]
P93313	1.7e-16	83.05	NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE...	[more]
P27572	2.8e-16	83.05	NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 ...	[more]
P26848	8.2e-16	79.66	NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 P...	[more]
P48915	8.8e-10	61.02	NADH-ubiquinone oxidoreductase chain 4 OS=Chondrus crispus OX=2769 GN=ND4 PE=3 S...	[more]

BLAST of MC02g0830.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6621657.1	4.13e-21	88.14	hypothetical protein I3842_Q007400, partial [Carya illinoinensis]	[more]
WP_131796663.1	6.94e-21	88.14	hypothetical protein [Candidatus Frankia datiscae]	[more]
TXG46259.1	9.75e-21	88.14	hypothetical protein EZV62_028290 [Acer yangbiense]	[more]
TIV33366.1	1.15e-20	84.75	hypothetical protein E5V91_33190, partial [Mesorhizobium sp.]	[more]
KAB5511270.1	2.34e-20	88.14	hypothetical protein DKX38_030065 [Salix brachista]	[more]

BLAST of MC02g0830.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5C7GP56	4.72e-21	88.14	Proton_antipo_M domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62...	[more]
A0A529UU08	5.58e-21	84.75	Proton_antipo_M domain-containing protein (Fragment) OS=Mesorhizobium sp. OX=187...	[more]
A0A5N5IZ13	1.13e-20	88.14	NADH-ubiquinone oxidoreductase chain 4 OS=Salix brachista OX=2182728 GN=DKX38_03...	[more]
A0A223GFW0	1.31e-20	84.75	NADH-ubiquinone oxidoreductase chain 4 (Fragment) OS=Sisyrinchium pachyrhizum su...	[more]
A0A223GFV9	1.31e-20	84.75	NADH-ubiquinone oxidoreductase chain 4 (Fragment) OS=Sisyrinchium marginatum OX=...	[more]

BLAST of MC02g0830.1 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00580.1	4.8e-19	86.44	NADH dehydrogenase subunit 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR43507:SF13	NADH-QUINONE OXIDOREDUCTASE PROTEIN	coord: 1..58
IPR003918	NADH:ubiquinone oxidoreductase	PANTHER	PTHR43507	NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4	coord: 1..58

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC02g0830	MC02g0830	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC02g0830.1-cds	MC02g0830.1-cds-MC02:6597275..6597328	CDS
MC02g0830.1-cds	MC02g0830.1-cds-MC02:6597331..6597450	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC02g0830.1	MC02g0830.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042773	ATP synthesis coupled electron transport
biological_process	GO:0015990	electron transport coupled proton transport
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031966	mitochondrial membrane
cellular_component	GO:0070469	respirasome
cellular_component	GO:0009579	thylakoid
molecular_function	GO:0008137	NADH dehydrogenase (ubiquinone) activity
molecular_function	GO:0048039	ubiquinone binding