MC02g0249.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC02g0249.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiontranscription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR
LocationMC02: 2321646 .. 2323089 (-)
Sequence length1119
RNA-Seq ExpressionMC02g0249.1
SyntenyMC02g0249.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCCCCTGTTTATAAGTGAAGCCCATTAATCATCACTTTATCCAATCCATTCATTCAAAACTTGAGATTTCAAACTTCAAACACCATAATGTTTGACATGGAGCCAATTGGGAAGCCATTAATGATGAACATCAACGACTTCGAGCTCCAAGATTTCATTCACGGCGACAACTTTGATCAATACGTGAATCTCATTCGTGGGGAGCCCGAAAATCCAACATTCAACTTTGATCTTGAATACATGCTAGATTCCAATATTGTCATGAATTCTGATCCCAATTCTCTGTTCGGTTCATTGGAGAGTTTCAACAGTATTATGAGAGAAGTTGAAGATGAAGACGAAGACGATACTTCTGTGGAGAATTCTTCCATCACAACAACAACAAAGAAGCCAAAAGTTGACAGATCAAGAACTTTGATTTCTGAAAGAAGGAGGAGGGGAAGAATGAAGGAAAAACTTTATGCATTACGCTCATTGGTTCCAAATATAACCAAGGTATGGCAATTGGCAAATACCCACATTTAGAATCATAATTCAATTTGTCCAATGATTTGATATTGGATAGAAAATTTGTGAAAATGTAGATGGATAAGGCTTCCATTGTTGGAGATGCAGTGTTGTATGTGAAAGAGCTTCAAATGCAGGCCAAGAAGCTCAAAGCTGAGATATCAGTTTTGGAATCCTCCATTAATGAGATACAAACACACCATCGACCAACTGATCAAAGCCACAGAAAGATTCAAACTTCACGCTCTGACCAGTTCCTCCCCAACAAGATAATTCAGGCAAGTTTTTAGGAAATTTCATGTTCTGTTCTCTGTAGTGATGTTCTGTTTTTTTCAGCTGCTAATTTGGAATCTTGGGTTTCAGCTGGATGTGTTTCAAGTGGAGGAAAGAGGGTTTTATTTGAGGATGGTGTGCAAGATGGGTGAAGGAGTAGCCATGTCCCTTTACAAGGTTCTTGAATCTCTTACCAGTTTCAATATCCAAAGCTCCAACTTGGCCTCTGCCTCTGACAGATTCATCCTCACTGCTACAATCAATGTGATTAACTTCTTATGATTTGAATTGAAATGACATGAAAAGAGGATCAAAATTGTCCAACCTTTTTCATGTCAAGTTTTAAAATGTTTCTTGAGATGGGAATTTTTGGTTGCTTCCCATTTTCTGATGTTTTGGCATTGTAATGTTTGTGCAGGTGAGAGATTGTGAGGTGGACATGAACTTGCCAAATATGAAGCTTTGGCTTACTGGGGCACTTCTTAACCATGGATTTGAGTTCTAATCACATCCTAATCTCTCTCAGTTTAACCCTTTAAAACCAATACAGAGATTGTAAGTGTTTTAATGGGCAACTCTATACTGGAAGATGACTACAAGCCCATTTGAAGAATAATACTTGAGTGTTTACCAATCGTATTCATTTCTTTTTTACTACTA

mRNA sequence

ATTTTCCCCTGTTTATAAGTGAAGCCCATTAATCATCACTTTATCCAATCCATTCATTCAAAACTTGAGATTTCAAACTTCAAACACCATAATGTTTGACATGGAGCCAATTGGGAAGCCATTAATGATGAACATCAACGACTTCGAGCTCCAAGATTTCATTCACGGCGACAACTTTGATCAATACGTGAATCTCATTCGTGGGGAGCCCGAAAATCCAACATTCAACTTTGATCTTGAATACATGCTAGATTCCAATATTGTCATGAATTCTGATCCCAATTCTCTGTTCGGTTCATTGGAGAGTTTCAACAGTATTATGAGAGAAGTTGAAGATGAAGACGAAGACGATACTTCTGTGGAGAATTCTTCCATCACAACAACAACAAAGAAGCCAAAAGTTGACAGATCAAGAACTTTGATTTCTGAAAGAAGGAGGAGGGGAAGAATGAAGGAAAAACTTTATGCATTACGCTCATTGGTTCCAAATATAACCAAGATGGATAAGGCTTCCATTGTTGGAGATGCAGTGTTGTATGTGAAAGAGCTTCAAATGCAGGCCAAGAAGCTCAAAGCTGAGATATCAGTTTTGGAATCCTCCATTAATGAGATACAAACACACCATCGACCAACTGATCAAAGCCACAGAAAGATTCAAACTTCACGCTCTGACCAGTTCCTCCCCAACAAGATAATTCAGGCAACTGATGTGTTTCAAGTGGAGGAAAGAGGGTTTTATTTGAGGATGGTGTGCAAGATGGGTGAAGGAGTAGCCATGTCCCTTTACAAGGTTCTTGAATCTCTTACCAGTTTCAATATCCAAAGCTCCAACTTGGCCTCTGCCTCTGACAGATTCATCCTCACTGCTACAATCAATGTGAGAGATTGTGAGGTGGACATGAACTTGCCAAATATGAAGCTTTGGCTTACTGGGGCACTTCTTAACCATGGATTTGAGTTCTAATCACATCCTAATCTCTCTCAGTTTAACCCTTTAAAACCAATACAGAGATTGTAAGTGTTTTAATGGGCAACTCTATACTGGAAGATGACTACAAGCCCATTTGAAGAATAATACTTGAGTGTTTACCAATCGTATTCATTTCTTTTTTACTACTA

Coding sequence (CDS)

ATGTTTGACATGGAGCCAATTGGGAAGCCATTAATGATGAACATCAACGACTTCGAGCTCCAAGATTTCATTCACGGCGACAACTTTGATCAATACGTGAATCTCATTCGTGGGGAGCCCGAAAATCCAACATTCAACTTTGATCTTGAATACATGCTAGATTCCAATATTGTCATGAATTCTGATCCCAATTCTCTGTTCGGTTCATTGGAGAGTTTCAACAGTATTATGAGAGAAGTTGAAGATGAAGACGAAGACGATACTTCTGTGGAGAATTCTTCCATCACAACAACAACAAAGAAGCCAAAAGTTGACAGATCAAGAACTTTGATTTCTGAAAGAAGGAGGAGGGGAAGAATGAAGGAAAAACTTTATGCATTACGCTCATTGGTTCCAAATATAACCAAGATGGATAAGGCTTCCATTGTTGGAGATGCAGTGTTGTATGTGAAAGAGCTTCAAATGCAGGCCAAGAAGCTCAAAGCTGAGATATCAGTTTTGGAATCCTCCATTAATGAGATACAAACACACCATCGACCAACTGATCAAAGCCACAGAAAGATTCAAACTTCACGCTCTGACCAGTTCCTCCCCAACAAGATAATTCAGGCAACTGATGTGTTTCAAGTGGAGGAAAGAGGGTTTTATTTGAGGATGGTGTGCAAGATGGGTGAAGGAGTAGCCATGTCCCTTTACAAGGTTCTTGAATCTCTTACCAGTTTCAATATCCAAAGCTCCAACTTGGCCTCTGCCTCTGACAGATTCATCCTCACTGCTACAATCAATGTGAGAGATTGTGAGGTGGACATGAACTTGCCAAATATGAAGCTTTGGCTTACTGGGGCACTTCTTAACCATGGATTTGAGTTCTAA

Protein sequence

MFDMEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF
Homology
BLAST of MC02g0249.1 vs. ExPASy Swiss-Prot
Match: Q0V7X4 (Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Arabidopsis thaliana OX=3702 GN=FIT PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.7e-67
Identity = 164/309 (53.07%), Postives = 205/309 (66.34%), Query Frame = 0

Query: 12  MMNINDFELQDFIHGDNFDQYVNLIRGE----PENPTFNFDL------EYMLDSNIVMNS 71
           + NIND EL +F+   NFDQ++NLIRG+     ENP  +FDL         +D N  + +
Sbjct: 8   LSNINDLELHNFLVDPNFDQFINLIRGDHQTIDENPVLDFDLGPLQNSPCFIDENQFIPT 67

Query: 72  DPNSLFGSL--------ESF-----NSIMREVEDEDEDDTSVENSSITTT----TKKPKV 131
             + LF  L        ESF     +S+    E+++ED    ++SS TTT    T+K K 
Sbjct: 68  PVDDLFDELPDLDSNVAESFRSFDGDSVRAGGEEDEEDYNDGDDSSATTTNNDGTRKTKT 127

Query: 132 DRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI 191
           DRSRTLISERRRRGRMK+KLYALRSLVPNITKMDKASIVGDAVLYV+ELQ QAKKLK++I
Sbjct: 128 DRSRTLISERRRRGRMKDKLYALRSLVPNITKMDKASIVGDAVLYVQELQSQAKKLKSDI 187

Query: 192 SVLESSINEI--QTHHRPTDQSHRKIQTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCK 251
           + LE+S+N       H P  Q   K Q  R      +K I   DV QVEE+GFY+R+VC 
Sbjct: 188 AGLEASLNSTGGYQEHAPDAQ---KTQPFRGINPPASKKIIQMDVIQVEEKGFYVRLVCN 247

Query: 252 MGEGVAMSLYKVLESLTSFNIQSSNLASAS-DRFILTATINVRDCEVDMNLPNMKLWLTG 291
            GEGVA SLYK LESLTSF +Q+SNL+S S D ++LT T++    E  +NLPN+KLW+TG
Sbjct: 248 KGEGVAPSLYKSLESLTSFQVQNSNLSSPSPDTYLLTYTLDGTCFEQSLNLPNLKLWITG 307

BLAST of MC02g0249.1 vs. ExPASy Swiss-Prot
Match: A0A0N7KIY3 (Transcription factor BHLH156 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH156 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.4e-25
Identity = 84/223 (37.67%), Postives = 125/223 (56.05%), Query Frame = 0

Query: 97  TTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQ 156
           + T+K + DRS+T++SER+RR RMKEKLY LR+LVPNITKMDKASI+ DAV+YVK+LQ  
Sbjct: 124 SATRKRR-DRSKTIVSERKRRVRMKEKLYELRALVPNITKMDKASIIADAVVYVKDLQAH 183

Query: 157 AKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKIIQATD---------- 216
           A+KLK E++ LE +   I+    P+  + R  +  R       ++ +A D          
Sbjct: 184 ARKLKEEVAALEEA-RPIRP-PPPSAAAQRPQRQPRRVAAAAAQLARAADAAAVTTAAAA 243

Query: 217 ----------VFQVEERGFYLRMVCKM--------GEGVAMSLYKVLESLTSFNIQSSNL 276
                       QV E  F++ + C+         G GVA  +   +ESL+ F ++SS +
Sbjct: 244 PHGARVAHVGAAQVGEGRFFVTVECEPAAAAARGGGGGVAAPVCAAVESLSCFTVESSTV 303

Query: 277 ASASDRFILTATINVRDCEVDMNLPN---MKLWLTGALLNHGF 289
             + DR + T T+ V + E D++  +   +KLW+  ALL  GF
Sbjct: 304 GCSPDRVVATLTLKVSEAEEDVSAISECTVKLWVMAALLKEGF 343

BLAST of MC02g0249.1 vs. ExPASy Swiss-Prot
Match: Q9ZVX2 (Transcription factor ABORTED MICROSPORES OS=Arabidopsis thaliana OX=3702 GN=AMS PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.7e-14
Identity = 64/206 (31.07%), Postives = 109/206 (52.91%), Query Frame = 0

Query: 52  MLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLI 111
           +++   V+N    +  G ++S +    +++DED+              K  K  +++ L+
Sbjct: 267 LVNEQQVVNDKDMNENGRVDSGSDCSDQIDDEDDPKYK---------KKSGKGSQAKNLM 326

Query: 112 SERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI---SVLE 171
           +ERRRR ++ ++LYALRSLVP ITK+D+ASI+GDA+ YVKELQ +AK+L+ E+   S  E
Sbjct: 327 AERRRRKKLNDRLYALRSLVPRITKLDRASILGDAINYVKELQNEAKELQDELEENSETE 386

Query: 172 SSINEIQ----------THHRPTDQSHRKIQTSRSDQFLPN------KIIQATDVFQVEE 231
              N  Q          T   P    +  + + + D  L N      ++    DV Q++ 
Sbjct: 387 DGSNRPQGGMSLNGTVVTGFHPGLSCNSNVPSVKQDVDLENSNDKGQEMEPQVDVAQLDG 446

Query: 232 RGFYLRMVCKMGEGVAMSLYKVLESL 239
           R F+++++C+   G    L + L+SL
Sbjct: 447 REFFVKVICEYKPGGFTRLMEALDSL 463

BLAST of MC02g0249.1 vs. ExPASy Swiss-Prot
Match: Q2HIV9 (Transcription factor bHLH35 OS=Arabidopsis thaliana OX=3702 GN=BHLH35 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 5.0e-13
Identity = 65/194 (33.51%), Postives = 101/194 (52.06%), Query Frame = 0

Query: 83  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 142
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 33  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 92

Query: 143 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRS----DQFLP 202
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+     D    
Sbjct: 93  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 152

Query: 203 NKIIQATD--VFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFI 262
             +I+  +  V  + ER   + + C       + L +V ESL +  I +SNL S S    
Sbjct: 153 TSLIEVLELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTSNLTSFSGMIF 212

Query: 263 LTATINVRDCEVDM 271
            T  I   + E ++
Sbjct: 213 HTVFIEADEEEQEV 222

BLAST of MC02g0249.1 vs. ExPASy Swiss-Prot
Match: Q700E3 (Transcription factor bHLH27 OS=Arabidopsis thaliana OX=3702 GN=BHLH27 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.3e-10
Identity = 62/200 (31.00%), Postives = 104/200 (52.00%), Query Frame = 0

Query: 83  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 142
           E+    S E+SS       P    S+ ++SER RR ++ ++L+ALRS+VPNI+K+DKAS+
Sbjct: 31  EEAFSGSGESSSPDGAATSPA--SSKNVVSERNRRQKLNQRLFALRSVVPNISKLDKASV 90

Query: 143 VGDAVLYVKELQMQAKKLKAEISVLES------------SINEIQTHHRPTDQSH----- 202
           + D++ Y++EL  Q K L+AEI  LES              N  +TH +    ++     
Sbjct: 91  IKDSIDYMQELIDQEKTLEAEIRELESRSTLLENPVRDYDCNFAETHLQDFSDNNDMRSK 150

Query: 203 --RKIQTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNI 262
             +++  S   Q  P ++++   V  + E+   + + C       + L KVLESL + NI
Sbjct: 151 KFKQMDYSTRVQHYPIEVLE-MKVTWMGEKTVVVCITCSKKRETMVQLCKVLESL-NLNI 210

Query: 263 QSSNLASASDRFILTATINV 264
            ++N +S + R   T  + V
Sbjct: 211 LTTNFSSFTSRLSTTLFLQV 226

BLAST of MC02g0249.1 vs. NCBI nr
Match: XP_022140694.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Momordica charantia])

HSP 1 Score: 550 bits (1417), Expect = 6.31e-197
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 1   MFDMEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMN 60
           MFDMEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMN
Sbjct: 1   MFDMEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMN 60

Query: 61  SDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRM 120
           SDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRM
Sbjct: 61  SDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRM 120

Query: 121 KEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRP 180
           KEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRP
Sbjct: 121 KEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRP 180

Query: 181 TDQSHRKIQTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTS 240
           TDQSHRKIQTSRSDQFLPNKIIQ  DVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTS
Sbjct: 181 TDQSHRKIQTSRSDQFLPNKIIQL-DVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTS 240

Query: 241 FNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF 290
           FNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF
Sbjct: 241 FNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF 289

BLAST of MC02g0249.1 vs. NCBI nr
Match: XP_022943777.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like [Cucurbita moschata] >XP_023513372.1 transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucurbita pepo subsp. pepo] >KAG6570822.1 Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010664.1 Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 445 bits (1144), Expect = 3.65e-155
Identity = 253/304 (83.22%), Postives = 267/304 (87.83%), Query Frame = 0

Query: 4   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 63
           ME +GK LMMNIN+FELQDFIHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDFIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 64  EYMLDSNIVMNSDPNSLFGSLESFNSIMREVE--DEDEDDTSVENSSITTTTKKPKVDRS 123
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EVE  DEDEDDTSVENSS+TT+ KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVENEDEDEDDTSVENSSVTTS-KKSKVDRS 120

Query: 124 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 183
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 184 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEG 243
           ESSINE QT HR   Q+H+KI QTS S QFLP KIIQ  DVFQVEERGFYLR+VCKMGE 
Sbjct: 181 ESSINETQTSHR--GQNHKKIVQTSHSGQFLPCKIIQL-DVFQVEERGFYLRLVCKMGER 240

Query: 244 VAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNH 290
           VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DMNLPN+KLWLTGALLNH
Sbjct: 241 VAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDMNLPNLKLWLTGALLNH 300

BLAST of MC02g0249.1 vs. NCBI nr
Match: XP_022986352.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like [Cucurbita maxima])

HSP 1 Score: 436 bits (1122), Expect = 8.15e-152
Identity = 249/304 (81.91%), Postives = 264/304 (86.84%), Query Frame = 0

Query: 4   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 63
           ME +GK LMMNIN+FELQD IHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDLIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 64  EYMLDSNIVMNSDPNSLFGSLESFNSIMREVE--DEDEDDTSVENSSITTTTKKPKVDRS 123
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EVE  DEDEDD SVENSS+TT+ KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVETEDEDEDDASVENSSVTTS-KKSKVDRS 120

Query: 124 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 183
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 184 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEG 243
           ESSINE QT +R   Q+H+ I QTS S QFLP KIIQ  DVFQVEERGFYLR+VCKMGE 
Sbjct: 181 ESSINETQTSYR--GQNHKNIVQTSHSGQFLPCKIIQL-DVFQVEERGFYLRLVCKMGER 240

Query: 244 VAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNH 290
           VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DMNLPN+KLWLTGALLNH
Sbjct: 241 VAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDMNLPNLKLWLTGALLNH 300

BLAST of MC02g0249.1 vs. NCBI nr
Match: XP_004145819.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucumis sativus] >KAE8646895.1 hypothetical protein Csa_020756 [Cucumis sativus])

HSP 1 Score: 424 bits (1091), Expect = 5.46e-147
Identity = 246/311 (79.10%), Postives = 262/311 (84.24%), Query Frame = 0

Query: 4   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTFN--FDL----------- 63
           ME  G+ LM +IND FELQDFIHGDNFDQYVNLIRG  E P FN  FDL           
Sbjct: 1   MEQFGRHLMTDINDQFELQDFIHGDNFDQYVNLIRGGNETPIFNNNFDLDFMNGCLIENR 60

Query: 64  ------EYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDT---SVENSSITTTTK 123
                 EY++DSN+VMNSDPNSLF +LESFNSIM+EVEDEDED+    SVENSS +TT+K
Sbjct: 61  VVDQSLEYIVDSNMVMNSDPNSLFSTLESFNSIMKEVEDEDEDEDENESVENSS-STTSK 120

Query: 124 KPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKL 183
           KPK DR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKL
Sbjct: 121 KPKADRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKL 180

Query: 184 KAEISVLESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQATDVFQVEERGFYLRM 243
           K+EISVLESSINE Q  HR  DQ+ +KI QTS SDQFLP KIIQ  DVFQVEERGFYLR+
Sbjct: 181 KSEISVLESSINETQKVHR--DQTKKKIIQTSYSDQFLPTKIIQL-DVFQVEERGFYLRL 240

Query: 244 VCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWL 290
           VCKMGE VAMSLYKVLESLTSF IQSSNL SASDRFILTATINVRDCEVDMNLPN+KLWL
Sbjct: 241 VCKMGERVAMSLYKVLESLTSFIIQSSNLTSASDRFILTATINVRDCEVDMNLPNLKLWL 300

BLAST of MC02g0249.1 vs. NCBI nr
Match: XP_038902250.1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Benincasa hispida])

HSP 1 Score: 424 bits (1091), Expect = 5.46e-147
Identity = 245/309 (79.29%), Postives = 262/309 (84.79%), Query Frame = 0

Query: 4   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTFN-FD------------- 63
           ME IG+ LM NI+D FELQDFIHGDNFDQYVNLIRG  EN  FN FD             
Sbjct: 1   MEQIGRHLMTNISDQFELQDFIHGDNFDQYVNLIRGGHENQIFNNFDPDFMNSLLVENGA 60

Query: 64  ---LEYMLDSNIVMN--SDPNSLFGSLESFNSIMREVE--DEDEDDTSVENSSITTTTKK 123
               E+++DSN+VMN  SDPNSLF +LESFNSIM+EVE  DEDEDDTSVENSS  T +KK
Sbjct: 61  DPSAEFIVDSNMVMNNNSDPNSLFSTLESFNSIMKEVENDDEDEDDTSVENSSSFTNSKK 120

Query: 124 PKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLK 183
           PKVDR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLK
Sbjct: 121 PKVDRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLK 180

Query: 184 AEISVLESSINEIQTHHRPTDQSHRKIQTSRSDQFLPNKIIQATDVFQVEERGFYLRMVC 243
           AEISVLESSINE QT+HR  +Q +  IQT  S+QFLP KIIQ  DVFQVEERGFYLR+VC
Sbjct: 181 AEISVLESSINETQTNHRLQNQKNI-IQTPHSEQFLPTKIIQL-DVFQVEERGFYLRLVC 240

Query: 244 KMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTG 290
           KMGE VAMSLYKVLESLTSF IQSSNLASASDRFILTATINVRDCEV+MNLPN+KLWLTG
Sbjct: 241 KMGERVAMSLYKVLESLTSFIIQSSNLASASDRFILTATINVRDCEVEMNLPNLKLWLTG 300

BLAST of MC02g0249.1 vs. ExPASy TrEMBL
Match: A0A6J1CGE1 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Momordica charantia OX=3673 GN=LOC111011295 PE=4 SV=1)

HSP 1 Score: 550 bits (1417), Expect = 3.06e-197
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 1   MFDMEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMN 60
           MFDMEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMN
Sbjct: 1   MFDMEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTFNFDLEYMLDSNIVMN 60

Query: 61  SDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRM 120
           SDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRM
Sbjct: 61  SDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRM 120

Query: 121 KEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRP 180
           KEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRP
Sbjct: 121 KEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRP 180

Query: 181 TDQSHRKIQTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTS 240
           TDQSHRKIQTSRSDQFLPNKIIQ  DVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTS
Sbjct: 181 TDQSHRKIQTSRSDQFLPNKIIQL-DVFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTS 240

Query: 241 FNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF 290
           FNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF
Sbjct: 241 FNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNHGFEF 289

BLAST of MC02g0249.1 vs. ExPASy TrEMBL
Match: A0A6J1FTZ6 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like OS=Cucurbita moschata OX=3662 GN=LOC111448427 PE=4 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 1.77e-155
Identity = 253/304 (83.22%), Postives = 267/304 (87.83%), Query Frame = 0

Query: 4   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 63
           ME +GK LMMNIN+FELQDFIHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDFIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 64  EYMLDSNIVMNSDPNSLFGSLESFNSIMREVE--DEDEDDTSVENSSITTTTKKPKVDRS 123
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EVE  DEDEDDTSVENSS+TT+ KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVENEDEDEDDTSVENSSVTTS-KKSKVDRS 120

Query: 124 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 183
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 184 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEG 243
           ESSINE QT HR   Q+H+KI QTS S QFLP KIIQ  DVFQVEERGFYLR+VCKMGE 
Sbjct: 181 ESSINETQTSHR--GQNHKKIVQTSHSGQFLPCKIIQL-DVFQVEERGFYLRLVCKMGER 240

Query: 244 VAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNH 290
           VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DMNLPN+KLWLTGALLNH
Sbjct: 241 VAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDMNLPNLKLWLTGALLNH 300

BLAST of MC02g0249.1 vs. ExPASy TrEMBL
Match: A0A6J1J7B0 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like OS=Cucurbita maxima OX=3661 GN=LOC111484117 PE=4 SV=1)

HSP 1 Score: 436 bits (1122), Expect = 3.95e-152
Identity = 249/304 (81.91%), Postives = 264/304 (86.84%), Query Frame = 0

Query: 4   MEPIGKPLMMNINDFELQDFIHGDNFDQYVNLIRGEPENPTF-NFDL------------- 63
           ME +GK LMMNIN+FELQD IHGDNFDQYVNLIRG  ENP F N DL             
Sbjct: 1   MEHVGKHLMMNINEFELQDLIHGDNFDQYVNLIRGGSENPIFDNLDLDFMNVDNRLDPSA 60

Query: 64  EYMLDSNIVMNSDPNSLFGSLESFNSIMREVE--DEDEDDTSVENSSITTTTKKPKVDRS 123
           EY+LDSNIVMNSDPNSLF SLESFNSIM+EVE  DEDEDD SVENSS+TT+ KK KVDRS
Sbjct: 61  EYILDSNIVMNSDPNSLFSSLESFNSIMKEVETEDEDEDDASVENSSVTTS-KKSKVDRS 120

Query: 124 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEISVL 183
           RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQ+QAKKLKAEISVL
Sbjct: 121 RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQVQAKKLKAEISVL 180

Query: 184 ESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCKMGEG 243
           ESSINE QT +R   Q+H+ I QTS S QFLP KIIQ  DVFQVEERGFYLR+VCKMGE 
Sbjct: 181 ESSINETQTSYR--GQNHKNIVQTSHSGQFLPCKIIQL-DVFQVEERGFYLRLVCKMGER 240

Query: 244 VAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLWLTGALLNH 290
           VAMSLYKVLESLTSF+IQSSNLASASDRFILTATINVR+CE+DMNLPN+KLWLTGALLNH
Sbjct: 241 VAMSLYKVLESLTSFSIQSSNLASASDRFILTATINVRNCEMDMNLPNLKLWLTGALLNH 300

BLAST of MC02g0249.1 vs. ExPASy TrEMBL
Match: A0A5A7T764 (Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3G00170 PE=4 SV=1)

HSP 1 Score: 422 bits (1085), Expect = 2.32e-146
Identity = 242/312 (77.56%), Postives = 261/312 (83.65%), Query Frame = 0

Query: 4   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTFN--FDL----------- 63
           ME  G+ LM +IND FELQDFIHGDNFD+YVNLIRG  E P FN  FDL           
Sbjct: 1   MEQFGRHLMTDINDQFELQDFIHGDNFDRYVNLIRGGNETPIFNNNFDLDFVNGCLIEDR 60

Query: 64  ------EYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSI----TTTT 123
                 EY++DSN+VMNSDPNSLF +LESFNSIM+EVE+EDED+   EN S+    +TT+
Sbjct: 61  VVDQSLEYIVDSNMVMNSDPNSLFSTLESFNSIMKEVENEDEDEDEDENESVENSSSTTS 120

Query: 124 KKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 183
           KKPK DR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK
Sbjct: 121 KKPKADRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 180

Query: 184 LKAEISVLESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQATDVFQVEERGFYLR 243
           LKAEISVLESSINE Q  HR  DQ+ +KI QTS SDQFLP KIIQ  DVFQVEERGFYLR
Sbjct: 181 LKAEISVLESSINETQKIHR--DQTKKKIIQTSYSDQFLPTKIIQL-DVFQVEERGFYLR 240

Query: 244 MVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLW 290
           +VCKMGE VAMSLYKVLESLT+F IQSSNLASASDRFILTATINVRDCEVDMNLPN+KLW
Sbjct: 241 LVCKMGERVAMSLYKVLESLTNFIIQSSNLASASDRFILTATINVRDCEVDMNLPNLKLW 300

BLAST of MC02g0249.1 vs. ExPASy TrEMBL
Match: A0A1S3CQB6 (transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503037 PE=4 SV=1)

HSP 1 Score: 422 bits (1085), Expect = 2.32e-146
Identity = 242/312 (77.56%), Postives = 261/312 (83.65%), Query Frame = 0

Query: 4   MEPIGKPLMMNIND-FELQDFIHGDNFDQYVNLIRGEPENPTFN--FDL----------- 63
           ME  G+ LM +IND FELQDFIHGDNFD+YVNLIRG  E P FN  FDL           
Sbjct: 1   MEQFGRHLMTDINDQFELQDFIHGDNFDRYVNLIRGGNETPIFNNNFDLDFVNGCLIEDR 60

Query: 64  ------EYMLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSI----TTTT 123
                 EY++DSN+VMNSDPNSLF +LESFNSIM+EVE+EDED+   EN S+    +TT+
Sbjct: 61  VVDQSLEYIVDSNMVMNSDPNSLFSTLESFNSIMKEVENEDEDEDEDENESVENSSSTTS 120

Query: 124 KKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 183
           KKPK DR+RTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK
Sbjct: 121 KKPKADRTRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKK 180

Query: 184 LKAEISVLESSINEIQTHHRPTDQSHRKI-QTSRSDQFLPNKIIQATDVFQVEERGFYLR 243
           LKAEISVLESSINE Q  HR  DQ+ +KI QTS SDQFLP KIIQ  DVFQVEERGFYLR
Sbjct: 181 LKAEISVLESSINETQKIHR--DQTKKKIIQTSYSDQFLPTKIIQL-DVFQVEERGFYLR 240

Query: 244 MVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFILTATINVRDCEVDMNLPNMKLW 290
           +VCKMGE VAMSLYKVLESLT+F IQSSNLASASDRFILTATINVRDCEVDMNLPN+KLW
Sbjct: 241 LVCKMGERVAMSLYKVLESLTNFIIQSSNLASASDRFILTATINVRDCEVDMNLPNLKLW 300

BLAST of MC02g0249.1 vs. TAIR 10
Match: AT2G28160.1 (FER-like regulator of iron uptake )

HSP 1 Score: 256.9 bits (655), Expect = 1.9e-68
Identity = 164/309 (53.07%), Postives = 205/309 (66.34%), Query Frame = 0

Query: 12  MMNINDFELQDFIHGDNFDQYVNLIRGE----PENPTFNFDL------EYMLDSNIVMNS 71
           + NIND EL +F+   NFDQ++NLIRG+     ENP  +FDL         +D N  + +
Sbjct: 8   LSNINDLELHNFLVDPNFDQFINLIRGDHQTIDENPVLDFDLGPLQNSPCFIDENQFIPT 67

Query: 72  DPNSLFGSL--------ESF-----NSIMREVEDEDEDDTSVENSSITTT----TKKPKV 131
             + LF  L        ESF     +S+    E+++ED    ++SS TTT    T+K K 
Sbjct: 68  PVDDLFDELPDLDSNVAESFRSFDGDSVRAGGEEDEEDYNDGDDSSATTTNNDGTRKTKT 127

Query: 132 DRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI 191
           DRSRTLISERRRRGRMK+KLYALRSLVPNITKMDKASIVGDAVLYV+ELQ QAKKLK++I
Sbjct: 128 DRSRTLISERRRRGRMKDKLYALRSLVPNITKMDKASIVGDAVLYVQELQSQAKKLKSDI 187

Query: 192 SVLESSINEI--QTHHRPTDQSHRKIQTSRSDQFLPNKIIQATDVFQVEERGFYLRMVCK 251
           + LE+S+N       H P  Q   K Q  R      +K I   DV QVEE+GFY+R+VC 
Sbjct: 188 AGLEASLNSTGGYQEHAPDAQ---KTQPFRGINPPASKKIIQMDVIQVEEKGFYVRLVCN 247

Query: 252 MGEGVAMSLYKVLESLTSFNIQSSNLASAS-DRFILTATINVRDCEVDMNLPNMKLWLTG 291
            GEGVA SLYK LESLTSF +Q+SNL+S S D ++LT T++    E  +NLPN+KLW+TG
Sbjct: 248 KGEGVAPSLYKSLESLTSFQVQNSNLSSPSPDTYLLTYTLDGTCFEQSLNLPNLKLWITG 307

BLAST of MC02g0249.1 vs. TAIR 10
Match: AT2G16910.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-15
Identity = 64/206 (31.07%), Postives = 109/206 (52.91%), Query Frame = 0

Query: 52  MLDSNIVMNSDPNSLFGSLESFNSIMREVEDEDEDDTSVENSSITTTTKKPKVDRSRTLI 111
           +++   V+N    +  G ++S +    +++DED+              K  K  +++ L+
Sbjct: 267 LVNEQQVVNDKDMNENGRVDSGSDCSDQIDDEDDPKYK---------KKSGKGSQAKNLM 326

Query: 112 SERRRRGRMKEKLYALRSLVPNITKMDKASIVGDAVLYVKELQMQAKKLKAEI---SVLE 171
           +ERRRR ++ ++LYALRSLVP ITK+D+ASI+GDA+ YVKELQ +AK+L+ E+   S  E
Sbjct: 327 AERRRRKKLNDRLYALRSLVPRITKLDRASILGDAINYVKELQNEAKELQDELEENSETE 386

Query: 172 SSINEIQ----------THHRPTDQSHRKIQTSRSDQFLPN------KIIQATDVFQVEE 231
              N  Q          T   P    +  + + + D  L N      ++    DV Q++ 
Sbjct: 387 DGSNRPQGGMSLNGTVVTGFHPGLSCNSNVPSVKQDVDLENSNDKGQEMEPQVDVAQLDG 446

Query: 232 RGFYLRMVCKMGEGVAMSLYKVLESL 239
           R F+++++C+   G    L + L+SL
Sbjct: 447 REFFVKVICEYKPGGFTRLMEALDSL 463

BLAST of MC02g0249.1 vs. TAIR 10
Match: AT5G57150.4 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.6e-14
Identity = 65/188 (34.57%), Postives = 99/188 (52.66%), Query Frame = 0

Query: 83  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 142
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 33  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 92

Query: 143 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRS----DQFLP 202
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+     D    
Sbjct: 93  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 152

Query: 203 NKIIQATD--VFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFI 262
             +I+  +  V  + ER   + + C       + L +V ESL +  I +SNL S S    
Sbjct: 153 TSLIEVLELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTSNLTSFSGMIF 212

Query: 263 LTATINVR 265
            T  I +R
Sbjct: 213 HTVFIELR 216

BLAST of MC02g0249.1 vs. TAIR 10
Match: AT5G57150.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 2.7e-14
Identity = 65/187 (34.76%), Postives = 98/187 (52.41%), Query Frame = 0

Query: 83  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 142
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 33  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 92

Query: 143 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRS----DQFLP 202
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+     D    
Sbjct: 93  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 152

Query: 203 NKIIQATD--VFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFI 262
             +I+  +  V  + ER   + + C       + L +V ESL +  I +SNL S S    
Sbjct: 153 TSLIEVLELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTSNLTSFSGMIF 212

Query: 263 LTATINV 264
            T  I V
Sbjct: 213 HTVFIEV 215

BLAST of MC02g0249.1 vs. TAIR 10
Match: AT5G57150.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 3.6e-14
Identity = 65/194 (33.51%), Postives = 101/194 (52.06%), Query Frame = 0

Query: 83  EDEDDTSVENSSITTTTKKPKVDRSRTLISERRRRGRMKEKLYALRSLVPNITKMDKASI 142
           E+    S ++SS       P    S+ ++SER RR ++ ++L+ALRS+VPNITKMDKASI
Sbjct: 32  EEAISGSYDSSSPDGAASSP---ASKNIVSERNRRQKLNQRLFALRSVVPNITKMDKASI 91

Query: 143 VGDAVLYVKELQMQAKKLKAEISVLESSINEIQTHHRPTDQSHRKIQTSRS----DQFLP 202
           + DA+ Y++ LQ + KKL+AEI  LES+     +  +  D+      TS+     D    
Sbjct: 92  IKDAISYIEGLQYEEKKLEAEIRELESTPKSSLSFSKDFDRDLLVPVTSKKMKQLDSGSS 151

Query: 203 NKIIQATD--VFQVEERGFYLRMVCKMGEGVAMSLYKVLESLTSFNIQSSNLASASDRFI 262
             +I+  +  V  + ER   + + C       + L +V ESL +  I +SNL S S    
Sbjct: 152 TSLIEVLELKVTFMGERTMVVSVTCNKRTDTMVKLCEVFESL-NLKILTSNLTSFSGMIF 211

Query: 263 LTATINVRDCEVDM 271
            T  I   + E ++
Sbjct: 212 HTVFIEADEEEQEV 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0V7X42.7e-6753.07Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Ar... [more]
A0A0N7KIY33.4e-2537.67Transcription factor BHLH156 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH156... [more]
Q9ZVX22.7e-1431.07Transcription factor ABORTED MICROSPORES OS=Arabidopsis thaliana OX=3702 GN=AMS ... [more]
Q2HIV95.0e-1333.51Transcription factor bHLH35 OS=Arabidopsis thaliana OX=3702 GN=BHLH35 PE=2 SV=1[more]
Q700E32.3e-1031.00Transcription factor bHLH27 OS=Arabidopsis thaliana OX=3702 GN=BHLH27 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022140694.16.31e-19799.31transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Momo... [more]
XP_022943777.13.65e-15583.22transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
XP_022986352.18.15e-15281.91transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
XP_004145819.15.46e-14779.10transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Cucu... [more]
XP_038902250.15.46e-14779.29transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR [Beni... [more]
Match NameE-valueIdentityDescription
A0A6J1CGE13.06e-19799.31transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR OS=Mo... [more]
A0A6J1FTZ61.77e-15583.22transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
A0A6J1J7B03.95e-15281.91transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like ... [more]
A0A5A7T7642.32e-14677.56Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isofo... [more]
A0A1S3CQB62.32e-14677.56transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR isofo... [more]
Match NameE-valueIdentityDescription
AT2G28160.11.9e-6853.07FER-like regulator of iron uptake [more]
AT2G16910.11.9e-1531.07basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G57150.41.6e-1434.57basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G57150.22.7e-1434.76basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G57150.13.6e-1433.51basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 150..177
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..108
NoneNo IPR availablePANTHERPTHR31945TRANSCRIPTION FACTOR SCREAM2-RELATEDcoord: 59..289
NoneNo IPR availablePANTHERPTHR31945:SF17TRANSCRIPTION FACTOR FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTORcoord: 59..289
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 110..159
e-value: 6.4E-13
score: 58.9
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 110..154
e-value: 2.3E-9
score: 37.1
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 104..153
score: 15.237004
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 95..184
e-value: 2.0E-15
score: 58.4
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 106..181

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC02g0249MC02g0249gene


The following utr3 feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC02g0249.1-utr3MC02g0249.1-utr3-MC02:2321646..2321800utr3


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC02g0249.1-cdsMC02g0249.1-cds-MC02:2321801..2321887CDS
MC02g0249.1-cdsMC02g0249.1-cds-MC02:2322042..2322212CDS
MC02g0249.1-cdsMC02g0249.1-cds-MC02:2322214..2322215CDS
MC02g0249.1-cdsMC02g0249.1-cds-MC02:2322297..2322501CDS
MC02g0249.1-cdsMC02g0249.1-cds-MC02:2322591..2322998CDS


The following utr5 feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC02g0249.1-utr5MC02g0249.1-utr5-MC02:2322999..2323089utr5


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC02g0249.1MC02g0249.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034756 regulation of iron ion transport
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0010039 response to iron ion
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding