MC02g0141.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC02g0141.1 vs. ExPASy Swiss-Prot
Match: P36146 (Protein LAS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LAS1 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.8e-12
Identity = 55/177 (31.07%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 25  KLVPWSTWSE------WLFVSQSIFSDSPDSVAAALRRVSTWRSRGC--LPVVVEVTAAI 84
           ++VPW  ++E      W +          D    A++RV ++R +G   LP VV+ TA I
Sbjct: 5   RIVPWRDFAELEELKLWFYPKSK--GTIEDKRQRAVQRVQSYRLKGSQYLPHVVDSTAQI 64

Query: 85  IEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMAIMRLVNGVVEKTRK-K 144
                         +    +D KE  L  V      + + Y MA++R VNG+++ T++ +
Sbjct: 65  --------------TCAVLLDEKEACL-GVHQDSIPIRLSYVMALIRFVNGLLDPTQQSQ 124

Query: 145 TEVSIAVAADAIGLPRVLIDIRHEGSH-RDLPALQVVRCASTKALRWLRSYYWEPQE 192
             + +   A  IGLP   +D+RH G+H RDLP L+++R A+ +AL WL  +YW  +E
Sbjct: 125 FAIPLHTLAAKIGLPSWFVDLRHWGTHERDLPGLEMLRWAANEALSWLYDHYWNDEE 164

BLAST of MC02g0141.1 vs. ExPASy Swiss-Prot
Match: Q9Y4W2 (Ribosomal biogenesis protein LAS1L OS=Homo sapiens OX=9606 GN=LAS1L PE=1 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 1.2e-11
Identity = 49/164 (29.88%), Postives = 77/164 (46.95%), Query Frame = 0

Query: 26  LVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTWRSRGC--LPVVVEVTAAIIEIQQKD 85
           +V W + +EW  V+  +F D       AL R++ WRSR    LP+ V  TA +I  +  D
Sbjct: 42  VVAWLSRAEWDQVTVYLFCDDHKLQRYALNRITVWRSRSGNELPLAVASTADLIRCKLLD 101

Query: 86  PYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVA 145
                       V G         L  + L +LY MA++R VN + E+  K  +V +   
Sbjct: 102 ------------VTG--------GLGTDELRLLYGMALVRFVNLISERKTKFAKVPLKCL 161

Query: 146 ADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALRWLRSYYW 188
           A  + +P  ++D+RHE +H+ +P +   R      L WL+  YW
Sbjct: 162 AQEVNIPDWIVDLRHELTHKKMPHINDCRRGCYFVLDWLQKTYW 185

BLAST of MC02g0141.1 vs. ExPASy Swiss-Prot
Match: O42936 (Pre-rRNA-processing protein las1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=las1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.6e-11
Identity = 49/202 (24.26%), Postives = 100/202 (49.50%), Query Frame = 0

Query: 25  KLVPWSTWSEWLFVSQSIFSDSPDSV--AAALRR----VSTWRSRGCLPVVVEVTAAIIE 84
           K+VPW    ++L++    +++  + +   AAL R    +  W +RG +P  VE T+ ++ 
Sbjct: 4   KVVPWRLKEDFLYLMSCFYNEGQEGIPDLAALFRGVEIIQAWSTRGRIPHSVESTSQLV- 63

Query: 85  IQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMAIMRLVNGVVEKTRK-KTE 144
                                    +++  ++ +  +   ++I R V+G+++  ++ +  
Sbjct: 64  -------------------------SSLISNDRSQKLALAVSISRFVSGLLDPIQQSQYA 123

Query: 145 VSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALRWLRSYYWEP---QEKAI 204
           + +AV A +I LP   +++RH  +H +LP+L V+R A+ +AL WL  +YW P    E   
Sbjct: 124 IPMAVLAKSIDLPTYFVELRHAITHEELPSLPVLRQAAQRALSWLYDHYWNPAATAESED 179

Query: 205 PFQGDISTTIRK-EIKSRLRDL 216
            +    +  + K EIK +++DL
Sbjct: 184 TYDYTETNELHKFEIKRKVKDL 179

BLAST of MC02g0141.1 vs. ExPASy Swiss-Prot
Match: A2BE28 (Ribosomal biogenesis protein LAS1L OS=Mus musculus OX=10090 GN=Las1l PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.3e-10
Identity = 49/180 (27.22%), Postives = 85/180 (47.22%), Query Frame = 0

Query: 13  ENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTWRSR--GCLPVVV 72
           EN+ E  S     +V W + +EW  V+  +F D       AL R++ WRSR    LP+ V
Sbjct: 15  ENQPESPSARG-IVVSWLSRAEWEQVTVYLFCDDHKLQQYALNRITVWRSRLGNELPLAV 74

Query: 73  EVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMAIMRLVNGVVE 132
             TA +          ++ + +DA+           +L  + L +LY MA++R VN + E
Sbjct: 75  ASTADL----------VRCKLIDAA----------GTLGTDELRLLYGMALVRFVNLISE 134

Query: 133 KTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALRWLRSYYWEPQ 191
           +  K + + +   A  + +P  ++++RH  +H+ +P +   R      L WL+  YW  Q
Sbjct: 135 RKTKCSNLPLKYLAQEVNIPDWIVELRHNLTHKKMPHINECRRGCYFVLNWLQKTYWSRQ 173

BLAST of MC02g0141.1 vs. NCBI nr
Match: XP_022153084.1 (uncharacterized protein LOC111020670 [Momordica charantia])

HSP 1 Score: 1167 bits (3018), Expect = 0.0
Identity = 609/609 (100.00%), Postives = 609/609 (100.00%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW
Sbjct: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK
Sbjct: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI
Sbjct: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG
Sbjct: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK
Sbjct: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKNLTNPVDG 480
           LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKNLTNPVDG
Sbjct: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKNLTNPVDG 480

Query: 481 NVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSNYS 540
           NVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSNYS
Sbjct: 481 NVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSNYS 540

Query: 541 HKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEELSA 600
           HKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEELSA
Sbjct: 541 HKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEELSA 600

Query: 601 IQAAVRILL 609
           IQAAVRILL
Sbjct: 601 IQAAVRILL 609

BLAST of MC02g0141.1 vs. NCBI nr
Match: XP_022957841.1 (uncharacterized protein LOC111459262 [Cucurbita moschata])

HSP 1 Score: 946 bits (2446), Expect = 0.0
Identity = 501/611 (82.00%), Postives = 549/611 (89.85%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           MESLLGFEEVPVE EE+ SSTYSRKLVPWSTWSEWLFVSQS+FSDS DSVAAAL R+STW
Sbjct: 1   MESLLGFEEVPVETEEDNSSTYSRKLVPWSTWSEWLFVSQSLFSDSSDSVAAALGRISTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTA+IIEIQQKDPYFIKNQS+DASV G E+QLN+VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSVDASVRGTEEQLNSVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHR+LPALQVVRCAS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRELPALQVVRCASIKALH 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WL++YYW+PQEKAIPF GDISTTIRKEIKSRLRDLAFNL  KQ+   GS +VKPKRLKTK
Sbjct: 181 WLKAYYWDPQEKAIPFLGDISTTIRKEIKSRLRDLAFNLSEKQDSQRGS-VVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKTLK L+QLYS+FSSEF+SVLLE LLKAMRSSDL   ENS+KSRS VL+GWKL IAKI
Sbjct: 241 TGKTLKFLIQLYSTFSSEFLSVLLEFLLKAMRSSDLAFPENSKKSRSAVLEGWKLAIAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLL+LLEA+LEKI+T+E LEYESQYF SSDH+M+SCQV+QLSSLFAWLV+KLKG
Sbjct: 301 SNKEPELLLDLLEAILEKIKTQEALEYESQYFTSSDHKMKSCQVAQLSSLFAWLVEKLKG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           +KLKR GEVF GKHIPKG+LME+LRKCLL SAPGNKRLMDSATDLAQL+   SL+KKLNK
Sbjct: 361 IKLKRNGEVFSGKHIPKGVLMELLRKCLLVSAPGNKRLMDSATDLAQLLDDGSLMKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKN--LTNPV 480
           LS LNL N   S +P+ E+P QTTS+N+ N VE S HEAS+KLE IKLHKRKN    NPV
Sbjct: 421 LSFLNLLN---SDIPDEENPNQTTSENNLNHVE-SLHEASRKLEMIKLHKRKNNLAANPV 480

Query: 481 DGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSN 540
           DGNVGSS+RWT+V+SWTPCPIGMLPR IGSSGRLPVLDLNPE+E  SE+ RSKEN EL  
Sbjct: 481 DGNVGSSTRWTVVNSWTPCPIGMLPRPIGSSGRLPVLDLNPENEPASEDLRSKENIELGK 540

Query: 541 YSHKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEEL 600
            S KREASSD QQLDS +VKK+KETN NFQ E +DVES +GVKGRLLIGGIWKKVGEEEL
Sbjct: 541 CSLKREASSDTQQLDSGTVKKMKETNGNFQYEMDDVESREGVKGRLLIGGIWKKVGEEEL 600

Query: 601 SAIQAAVRILL 609
           SAIQ+AVRIL+
Sbjct: 601 SAIQSAVRILI 606

BLAST of MC02g0141.1 vs. NCBI nr
Match: XP_023533035.1 (uncharacterized protein LOC111795041 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 500/611 (81.83%), Postives = 545/611 (89.20%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           MESLLGFEEVPVE EE+ SSTYSRKLVPWSTWSEWLFVSQS+FSDS DSVAAAL R+STW
Sbjct: 1   MESLLGFEEVPVETEEDNSSTYSRKLVPWSTWSEWLFVSQSLFSDSSDSVAAALGRISTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTA+IIEIQQKDPYFIKNQS+DASV G E+QLN+VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSVDASVRGTEEQLNSVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIA AADAIGLPRVLIDIRHEGSHR+LPALQVVRCAS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAAAADAIGLPRVLIDIRHEGSHRELPALQVVRCASIKALH 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WL+SYYWEPQEKAIPF GDISTTIRKEIKSRLRDLAFNL  KQN   GS +VKPKRLKTK
Sbjct: 181 WLKSYYWEPQEKAIPFLGDISTTIRKEIKSRLRDLAFNLNEKQNSQRGS-VVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKTLK L+QLYS+FSSEF+SVLLE LLKAMRSSDL   ENS+KSRS VL+GWKL IAKI
Sbjct: 241 TGKTLKFLIQLYSTFSSEFLSVLLEFLLKAMRSSDLAFPENSKKSRSAVLEGWKLAIAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLL+LLEA+LEKI+T E LEYESQYF SSDH+M+SCQV+QLSSLFAWLV+KLKG
Sbjct: 301 SNKEPELLLDLLEAILEKIKTEEALEYESQYFTSSDHKMKSCQVAQLSSLFAWLVEKLKG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           +KLKR GE   GKHIPKG+LME+LRKCLL SAPGNKRLMDSATDLAQL+   SL+KKLNK
Sbjct: 361 IKLKRNGEFCSGKHIPKGVLMELLRKCLLVSAPGNKRLMDSATDLAQLLDDGSLMKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKN--LTNPV 480
           LS LNL N   S +P+ E+P QTTS+ S N VE S  EAS+KLE IKLHKRKN    NPV
Sbjct: 421 LSFLNLLN---SDIPDEENPNQTTSEISLNHVE-SLREASRKLEMIKLHKRKNNLAANPV 480

Query: 481 DGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSN 540
           DGNVG+S+RWT+V+SWTPCPIGMLPR IGSSGRLPVLDLNPE+E  SE+ RSKEN EL  
Sbjct: 481 DGNVGTSTRWTVVNSWTPCPIGMLPRPIGSSGRLPVLDLNPENEPASEDLRSKENFELGK 540

Query: 541 YSHKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEEL 600
            SHKREASSD QQLDS +VKK+KETN NFQ+E +DVES +GVKGRLLIGGIWKKVGEEEL
Sbjct: 541 CSHKREASSDTQQLDSGTVKKMKETNGNFQSEIDDVESPEGVKGRLLIGGIWKKVGEEEL 600

Query: 601 SAIQAAVRILL 609
           SAIQ+AVRIL+
Sbjct: 601 SAIQSAVRILI 606

BLAST of MC02g0141.1 vs. NCBI nr
Match: XP_038902156.1 (pre-rRNA-processing protein las1 isoform X1 [Benincasa hispida] >XP_038902157.1 pre-rRNA-processing protein las1 isoform X1 [Benincasa hispida])

HSP 1 Score: 938 bits (2424), Expect = 0.0
Identity = 499/612 (81.54%), Postives = 552/612 (90.20%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           MESLLGF+EV VENEEE SSTYSRKLVPWSTWSEWLFV QS+FSDSPDSVAAAL R+STW
Sbjct: 1   MESLLGFQEVLVENEEENSSTYSRKLVPWSTWSEWLFVGQSLFSDSPDSVAAALSRISTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTA+I+EIQQKDPYFIKNQS++ASV G E+QL+NVSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIVEIQQKDPYFIKNQSMNASVCGTENQLSNVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHR+LPALQVVR AS KALR
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRELPALQVVRSASIKALR 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WL+SYYW+PQEKAIPFQGDISTTIRKEIKS+LRDLAFNLKVKQNP L SA+VKPKRLKTK
Sbjct: 181 WLKSYYWQPQEKAIPFQGDISTTIRKEIKSKLRDLAFNLKVKQNPELESALVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           +GKTLKVL+QLYS+FSSEFVSVLLE LLKAMRSSDL   ++S+KS S+VLDGWKL I+KI
Sbjct: 241 SGKTLKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLAFPKSSKKSSSSVLDGWKLAISKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLL+LLEA+LEKI+T+  LEYES+YFMSSDH+MESCQV+QLSSLFAWLV+KL G
Sbjct: 301 SNKEPELLLDLLEAILEKIKTQVALEYESKYFMSSDHKMESCQVAQLSSLFAWLVNKLNG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           +K KRKGEV  GKHIPK IL+E+LRKCLL SAP NKRLMDSATDLAQL+   SLVKKLNK
Sbjct: 361 IKPKRKGEVISGKHIPKSILVELLRKCLLVSAPCNKRLMDSATDLAQLLDDDSLVKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKN--LTNPV 480
           LS LN    P   +P+ E+P QT  +NSFNQVE S HEASQKLE IKLHKRKN    NPV
Sbjct: 421 LSFLNELISP---IPDEENPIQTMPENSFNQVE-SLHEASQKLEMIKLHKRKNNLAANPV 480

Query: 481 DGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSN 540
           DGNVGSSSRWT+VSSWTPCPIGMLPRSIG SGR  VL+LNPESE +SEE ++KENCEL N
Sbjct: 481 DGNVGSSSRWTVVSSWTPCPIGMLPRSIGCSGRPAVLNLNPESEPSSEELKTKENCELDN 540

Query: 541 YSHK-REASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEE 600
            S+K REASSDI+ LD  +VKK+KETNEN Q+ET++VES +GVKGRLLIGGIWKKVGEEE
Sbjct: 541 CSNKKREASSDIRHLDRSTVKKMKETNENVQSETQNVESPEGVKGRLLIGGIWKKVGEEE 600

Query: 601 LSAIQAAVRILL 609
           LSAIQ+AVRIL+
Sbjct: 601 LSAIQSAVRILI 608

BLAST of MC02g0141.1 vs. NCBI nr
Match: XP_008449642.1 (PREDICTED: pre-rRNA-processing protein las1 [Cucumis melo])

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 496/611 (81.18%), Postives = 546/611 (89.36%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           M+SLLGFEEV  ENEEE  STYSRKLVPWSTW+EWLFVSQS+FSDSPD VAAAL R+STW
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDFVAAALSRISTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTA+IIEIQQKDPYFIKNQS+DASV G E+QLN VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVRGTENQLNIVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPR+LIDIRHEGSHR+LPALQVVR AS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WL+SYYWEPQEKAIPFQGDIST+IRKEIKS+LR LAFNL+VKQNP L SA+VKPKRLKTK
Sbjct: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAFNLEVKQNPQLESALVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKT+KVL+QLYS+F SEFVSVLLE LLKAMRSSDL   +NS+KS S+VLDGWKL +AKI
Sbjct: 241 TGKTMKVLIQLYSTFPSEFVSVLLEFLLKAMRSSDLAFPKNSKKSSSSVLDGWKLTVAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLL+LLEAVLEKI+T+  LEYESQYFM SDH++ESCQV QLSSLFAWLVDKL G
Sbjct: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDHKVESCQVVQLSSLFAWLVDKLNG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           +KLKRKGEVF GKHIPKGILME+LRKCLL SAP NKRL+DSATDLAQL+   SLVKKLNK
Sbjct: 361 IKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNKRLLDSATDLAQLLDDDSLVKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKN--LTNPV 480
           LS +N+P  P   +P+GE+P QT S+NSFNQ E S  EAS+KLE IKL KRKN   +NPV
Sbjct: 421 LSFVNVPISP---IPDGENPNQTASENSFNQAE-SLREASRKLEMIKLRKRKNNLASNPV 480

Query: 481 DGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSN 540
           DGNVG SSRWT+VSSWTPCPIGMLPRSIG SGRL VLDLNPE+E  SEEPRSK+N  L+N
Sbjct: 481 DGNVGCSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLNPENEPASEEPRSKDNRALNN 540

Query: 541 YSHKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEEL 600
            SHKREASSDIQ LDS  VKK+KETN+  Q ETEDVES +G++GRLLIGGIWKKVGEEEL
Sbjct: 541 SSHKREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPEGIEGRLLIGGIWKKVGEEEL 600

Query: 601 SAIQAAVRILL 609
           SAIQ++VRIL+
Sbjct: 601 SAIQSSVRILI 607

BLAST of MC02g0141.1 vs. ExPASy TrEMBL
Match: A0A6J1DI01 (uncharacterized protein LOC111020670 OS=Momordica charantia OX=3673 GN=LOC111020670 PE=4 SV=1)

HSP 1 Score: 1167 bits (3018), Expect = 0.0
Identity = 609/609 (100.00%), Postives = 609/609 (100.00%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW
Sbjct: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK
Sbjct: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI
Sbjct: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG
Sbjct: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK
Sbjct: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKNLTNPVDG 480
           LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKNLTNPVDG
Sbjct: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKNLTNPVDG 480

Query: 481 NVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSNYS 540
           NVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSNYS
Sbjct: 481 NVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSNYS 540

Query: 541 HKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEELSA 600
           HKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEELSA
Sbjct: 541 HKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEELSA 600

Query: 601 IQAAVRILL 609
           IQAAVRILL
Sbjct: 601 IQAAVRILL 609

BLAST of MC02g0141.1 vs. ExPASy TrEMBL
Match: A0A6J1H0C5 (uncharacterized protein LOC111459262 OS=Cucurbita moschata OX=3662 GN=LOC111459262 PE=4 SV=1)

HSP 1 Score: 946 bits (2446), Expect = 0.0
Identity = 501/611 (82.00%), Postives = 549/611 (89.85%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           MESLLGFEEVPVE EE+ SSTYSRKLVPWSTWSEWLFVSQS+FSDS DSVAAAL R+STW
Sbjct: 1   MESLLGFEEVPVETEEDNSSTYSRKLVPWSTWSEWLFVSQSLFSDSSDSVAAALGRISTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTA+IIEIQQKDPYFIKNQS+DASV G E+QLN+VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSVDASVRGTEEQLNSVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHR+LPALQVVRCAS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRELPALQVVRCASIKALH 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WL++YYW+PQEKAIPF GDISTTIRKEIKSRLRDLAFNL  KQ+   GS +VKPKRLKTK
Sbjct: 181 WLKAYYWDPQEKAIPFLGDISTTIRKEIKSRLRDLAFNLSEKQDSQRGS-VVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKTLK L+QLYS+FSSEF+SVLLE LLKAMRSSDL   ENS+KSRS VL+GWKL IAKI
Sbjct: 241 TGKTLKFLIQLYSTFSSEFLSVLLEFLLKAMRSSDLAFPENSKKSRSAVLEGWKLAIAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLL+LLEA+LEKI+T+E LEYESQYF SSDH+M+SCQV+QLSSLFAWLV+KLKG
Sbjct: 301 SNKEPELLLDLLEAILEKIKTQEALEYESQYFTSSDHKMKSCQVAQLSSLFAWLVEKLKG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           +KLKR GEVF GKHIPKG+LME+LRKCLL SAPGNKRLMDSATDLAQL+   SL+KKLNK
Sbjct: 361 IKLKRNGEVFSGKHIPKGVLMELLRKCLLVSAPGNKRLMDSATDLAQLLDDGSLMKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKN--LTNPV 480
           LS LNL N   S +P+ E+P QTTS+N+ N VE S HEAS+KLE IKLHKRKN    NPV
Sbjct: 421 LSFLNLLN---SDIPDEENPNQTTSENNLNHVE-SLHEASRKLEMIKLHKRKNNLAANPV 480

Query: 481 DGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSN 540
           DGNVGSS+RWT+V+SWTPCPIGMLPR IGSSGRLPVLDLNPE+E  SE+ RSKEN EL  
Sbjct: 481 DGNVGSSTRWTVVNSWTPCPIGMLPRPIGSSGRLPVLDLNPENEPASEDLRSKENIELGK 540

Query: 541 YSHKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEEL 600
            S KREASSD QQLDS +VKK+KETN NFQ E +DVES +GVKGRLLIGGIWKKVGEEEL
Sbjct: 541 CSLKREASSDTQQLDSGTVKKMKETNGNFQYEMDDVESREGVKGRLLIGGIWKKVGEEEL 600

Query: 601 SAIQAAVRILL 609
           SAIQ+AVRIL+
Sbjct: 601 SAIQSAVRILI 606

BLAST of MC02g0141.1 vs. ExPASy TrEMBL
Match: A0A1S3BNF1 (pre-rRNA-processing protein las1 OS=Cucumis melo OX=3656 GN=LOC103491463 PE=4 SV=1)

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 496/611 (81.18%), Postives = 546/611 (89.36%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           M+SLLGFEEV  ENEEE  STYSRKLVPWSTW+EWLFVSQS+FSDSPD VAAAL R+STW
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDFVAAALSRISTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTA+IIEIQQKDPYFIKNQS+DASV G E+QLN VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVRGTENQLNIVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPR+LIDIRHEGSHR+LPALQVVR AS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIRHEGSHRELPALQVVRHASAKALC 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WL+SYYWEPQEKAIPFQGDIST+IRKEIKS+LR LAFNL+VKQNP L SA+VKPKRLKTK
Sbjct: 181 WLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAFNLEVKQNPQLESALVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKT+KVL+QLYS+F SEFVSVLLE LLKAMRSSDL   +NS+KS S+VLDGWKL +AKI
Sbjct: 241 TGKTMKVLIQLYSTFPSEFVSVLLEFLLKAMRSSDLAFPKNSKKSSSSVLDGWKLTVAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLL+LLEAVLEKI+T+  LEYESQYFM SDH++ESCQV QLSSLFAWLVDKL G
Sbjct: 301 SNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDHKVESCQVVQLSSLFAWLVDKLNG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           +KLKRKGEVF GKHIPKGILME+LRKCLL SAP NKRL+DSATDLAQL+   SLVKKLNK
Sbjct: 361 IKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNKRLLDSATDLAQLLDDDSLVKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKN--LTNPV 480
           LS +N+P  P   +P+GE+P QT S+NSFNQ E S  EAS+KLE IKL KRKN   +NPV
Sbjct: 421 LSFVNVPISP---IPDGENPNQTASENSFNQAE-SLREASRKLEMIKLRKRKNNLASNPV 480

Query: 481 DGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSN 540
           DGNVG SSRWT+VSSWTPCPIGMLPRSIG SGRL VLDLNPE+E  SEEPRSK+N  L+N
Sbjct: 481 DGNVGCSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVLDLNPENEPASEEPRSKDNRALNN 540

Query: 541 YSHKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEEL 600
            SHKREASSDIQ LDS  VKK+KETN+  Q ETEDVES +G++GRLLIGGIWKKVGEEEL
Sbjct: 541 SSHKREASSDIQHLDSSIVKKMKETNDKSQFETEDVESPEGIEGRLLIGGIWKKVGEEEL 600

Query: 601 SAIQAAVRILL 609
           SAIQ++VRIL+
Sbjct: 601 SAIQSSVRILI 607

BLAST of MC02g0141.1 vs. ExPASy TrEMBL
Match: A0A6J1K4K1 (uncharacterized protein LOC111490717 OS=Cucurbita maxima OX=3661 GN=LOC111490717 PE=4 SV=1)

HSP 1 Score: 933 bits (2412), Expect = 0.0
Identity = 495/611 (81.01%), Postives = 544/611 (89.03%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVSTW 60
           MESLLGFEEVPVE EE+ SSTYSRKLVPWSTWSEWLFVSQS+FSDS DSVAAAL R+STW
Sbjct: 1   MESLLGFEEVPVETEEDNSSTYSRKLVPWSTWSEWLFVSQSLFSDSSDSVAAALGRISTW 60

Query: 61  RSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAMLYCMA 120
           RSRGCLPVVVEVTA+IIEIQQKDPYF KNQS+DASV G E+ LN+VSLSEEALAMLYCMA
Sbjct: 61  RSRGCLPVVVEVTASIIEIQQKDPYFTKNQSVDASVRGTEEPLNSVSLSEEALAMLYCMA 120

Query: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCASTKALR 180
           IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHR+LPALQVVRCAS KAL 
Sbjct: 121 IMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRELPALQVVRCASIKALH 180

Query: 181 WLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKRLKTK 240
           WL+SYYWEPQEKAIPF GDISTTIRKEIKSRLRDLAFNL  KQN   GS +VKPKRLKTK
Sbjct: 181 WLKSYYWEPQEKAIPFLGDISTTIRKEIKSRLRDLAFNLNEKQNSQRGS-VVKPKRLKTK 240

Query: 241 TGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTVLDGWKLVIAKI 300
           TGKTLK L+QLYS+FSSEF+S+LLE LLKAMRSSDL   ENS+KSRSTVL+GWKL IAKI
Sbjct: 241 TGKTLKFLIQLYSTFSSEFLSILLEFLLKAMRSSDLAFPENSKKSRSTVLEGWKLAIAKI 300

Query: 301 SNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLVDKLKG 360
           SNKEPELLL+LLEA+LEKI+T+E LEYE QYF SSDH+M+SCQV+QLSSLFAWLV+KLKG
Sbjct: 301 SNKEPELLLDLLEAILEKIKTQEALEYEGQYFTSSDHKMKSCQVAQLSSLFAWLVEKLKG 360

Query: 361 LKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLVKKLNK 420
           + LKR GEV  GKHIPKG+LME+LRKCLL SAPGNKRLMDSATDLAQL+   SL+KKLNK
Sbjct: 361 INLKRNGEVSSGKHIPKGVLMELLRKCLLVSAPGNKRLMDSATDLAQLLDDGSLMKKLNK 420

Query: 421 LSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLHKRKN--LTNPV 480
           LS LNL N   S +P+ E+P QTTS+ S N VE S  EAS+KLE IKLHKRKN    NPV
Sbjct: 421 LSFLNLLN---SDIPDKENPIQTTSEISLNHVE-SLREASRKLEMIKLHKRKNNLAANPV 480

Query: 481 DGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKENCELSN 540
           DG VG+S+RWT+V+SWTPCPIGMLPR IGSSGRLPVLDLNPE+E  SE+ RS+EN EL  
Sbjct: 481 DGIVGTSTRWTVVNSWTPCPIGMLPRPIGSSGRLPVLDLNPENEPASEDLRSEENFELGK 540

Query: 541 YSHKREASSDIQQLDSCSVKKLKETNENFQAETEDVESAKGVKGRLLIGGIWKKVGEEEL 600
            SHKREASSD QQLDS +VKK+KETN NFQ+E +DV+S +GVKGRLLIGGIWKKVGEEEL
Sbjct: 541 CSHKREASSDTQQLDSGTVKKMKETNGNFQSEIDDVDSPEGVKGRLLIGGIWKKVGEEEL 600

Query: 601 SAIQAAVRILL 609
           SAIQ+AVRIL+
Sbjct: 601 SAIQSAVRILI 606

BLAST of MC02g0141.1 vs. ExPASy TrEMBL
Match: A0A5A7V7Q2 (Pre-rRNA-processing protein las1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold212G001390 PE=4 SV=1)

HSP 1 Score: 927 bits (2396), Expect = 0.0
Identity = 499/634 (78.71%), Postives = 547/634 (86.28%), Query Frame = 0

Query: 1   MESLLGFEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRRVS-- 60
           M+SLLGFEEV  ENEEE  STYSRKLVPWSTW+EWLFVSQS+FSDSPDSVAAAL RVS  
Sbjct: 1   MDSLLGFEEVLGENEEENLSTYSRKLVPWSTWNEWLFVSQSLFSDSPDSVAAALSRVSLL 60

Query: 61  ---------------------TWRSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVD 120
                                TWRSRGCLPVVVEVTA+IIEIQQKDPYFIKNQS+DASV 
Sbjct: 61  ISAACLCSVVLTFEVALGPISTWRSRGCLPVVVEVTASIIEIQQKDPYFIKNQSIDASVR 120

Query: 121 GKEDQLNNVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIR 180
           G E+QLN VSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPR+LIDIR
Sbjct: 121 GTENQLNIVSLSEEALAMLYCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRLLIDIR 180

Query: 181 HEGSHRDLPALQVVRCASTKALRWLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAF 240
           HEGSHR+LPALQVVR AS KAL WL+SYYWEPQEKAIPFQGDIST+IRKEIKS+LR LAF
Sbjct: 181 HEGSHRELPALQVVRHASAKALCWLKSYYWEPQEKAIPFQGDISTSIRKEIKSKLRVLAF 240

Query: 241 NLKVKQNPSLGSAMVKPKRLKTKTGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLM 300
           NL+VKQNP L SA+VKPKRLKTKTGK +KVL+QLYS+FSSEFVSVLLE LLKAMRSSDL 
Sbjct: 241 NLEVKQNPQLESALVKPKRLKTKTGKIMKVLIQLYSTFSSEFVSVLLEFLLKAMRSSDLA 300

Query: 301 CSENSQKSRSTVLDGWKLVIAKISNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDH 360
             +NS+KS S+VLDGWKL +AKISNKEPELLL+LLEAVLEKI+T+  LEYESQYFM SDH
Sbjct: 301 FPKNSKKSSSSVLDGWKLTVAKISNKEPELLLDLLEAVLEKIKTQVALEYESQYFMPSDH 360

Query: 361 RMESCQVSQLSSLFAWLVDKLKGLKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKR 420
           ++ESCQV QLSSLFAWLVDKL G+KLKRKGEVF GKHIPKGILME+LRKCLL SAP NKR
Sbjct: 361 KVESCQVVQLSSLFAWLVDKLNGIKLKRKGEVFSGKHIPKGILMELLRKCLLVSAPWNKR 420

Query: 421 LMDSATDLAQLMGAASLVKKLNKLSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFH 480
           L+DSATDLAQL+   SLVKKLNKLS +N+P  P   +P+GE+P QT S+NSFNQ E S  
Sbjct: 421 LLDSATDLAQLLDDDSLVKKLNKLSFVNVPISP---IPDGENPNQTASENSFNQAE-SLR 480

Query: 481 EASQKLEAIKLHKRKN--LTNPVDGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVL 540
           EAS+KLE IKL KRKN   +NPVDGNVG SSRWT+VSSWTPCPIGMLPRSIG SGRL VL
Sbjct: 481 EASRKLEMIKLRKRKNNLASNPVDGNVGGSSRWTVVSSWTPCPIGMLPRSIGCSGRLSVL 540

Query: 541 DLNPESENTSEEPRSKENCELSNYSHKREASSDIQQLDSCSVKKLKETNENFQAETEDVE 600
           DLNPE+E  SEEPRSKEN  L+N SHKREASSDIQ LDS  VKK+KETN+  Q ETEDVE
Sbjct: 541 DLNPENEPASEEPRSKENRALNNSSHKREASSDIQHLDSSIVKKMKETNDKSQFETEDVE 600

Query: 601 SAKGVKGRLLIGGIWKKVGEEELSAIQAAVRILL 609
           S +G++GRLLIGGIWKKVGEEELSAIQ++VRIL+
Sbjct: 601 SPEGIEGRLLIGGIWKKVGEEELSAIQSSVRILI 630

BLAST of MC02g0141.1 vs. TAIR 10
Match: AT5G12220.1 (las1-like family protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-108
Identity = 262/642 (40.81%), Postives = 375/642 (58.41%), Query Frame = 0

Query: 1   MESLLG----FEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRR 60
           ME+L G     E    E ++++SS    K VPW +W EW  V +S+FS SPD +A+AL R
Sbjct: 1   MEALAGIEADIESYFGEQDQQKSSPDGCKQVPWLSWEEWDSVRESLFSSSPDRIASALER 60

Query: 61  VSTWRSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAML 120
           V+TWRSRG LP  V+VT ++IEIQ KD +             +E Q  +   SE  L ML
Sbjct: 61  VATWRSRGSLPAPVDVTCSLIEIQLKDGFI-----------PREKQSADALYSEHLLQML 120

Query: 121 YCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCAST 180
           YCM I+RLVN V+EKTR++ +VSIA AA AIG+PR LID+RHEGSHR+LPAL V+R A+ 
Sbjct: 121 YCMGILRLVNCVIEKTRRREDVSIADAARAIGIPRKLIDLRHEGSHRELPALSVLRDAAD 180

Query: 181 KALRWLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKR 240
           +AL WL+SYYWE Q+  IP + D + +IR+E+KS+LR LAF+L++K+NP   S +VK K 
Sbjct: 181 EALEWLKSYYWEQQKYQIPLKRDGTASIRREVKSKLRKLAFSLQLKRNPQFDSPLVKEKC 240

Query: 241 LKTKTGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTV-LDGWKL 300
              +  K +  LV+LY SFS+E  SVLLE LLKA+ SS     +N       V LD WK 
Sbjct: 241 SNKRIRKIVSSLVELYPSFSAEISSVLLEFLLKALDSSKSTDLQNKAGQDFRVFLDEWKP 300

Query: 301 VIAKISNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLV 360
           VI ++SN+EPELLL LL+ VL+ I+  E   +E++  +      E  QV Q+  LFAWLV
Sbjct: 301 VIMELSNREPELLLTLLKEVLDMIQNNERRRHETEKPVE-----EVSQVEQVPFLFAWLV 360

Query: 361 DKLKGLKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLV 420
             L G K  ++      K  P   LME++R+CL+  A G + ++ SA  LA+++G   L 
Sbjct: 361 SLLNGSKHFQRNSSLEVKP-PSIFLMELIRRCLVLGALGYELVLKSAFLLAEIVGGRVLK 420

Query: 421 KKLNKLSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLH--KRKN 480
           +KL KL ++   +  ++ VP  +S T  T+  +  + E +   A ++LE +KL   K+K 
Sbjct: 421 EKLIKLPLM---HKSSAIVPLKQSSTLVTASTTLLEQEKNLGNAGKRLEFVKLQLSKKKG 480

Query: 481 LTNPVDGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKEN 540
           +     GN     RW    +W+PCPIGMLPR IGSSGRLP+LD N  +++ S++ +   N
Sbjct: 481 IDTDKTGN-----RWRKAKTWSPCPIGMLPRIIGSSGRLPLLD-NQNAKSISKQAQGNNN 540

Query: 541 CELSNYSHKREASSDIQQLDSCSVKKLKE----------TNENFQAETE-DVESA----- 600
                   KR A  +  QL+    K+ ++          T E+++ E E ++E A     
Sbjct: 541 A-------KRGAECNRNQLEKSPCKRARKSAGDSESNDVTLESYEEEAEMEIEHAYEETE 600

Query: 601 ------------KGVKGRLLIGGIWKKVGEEELSAIQAAVRI 608
                       +  +  L+I G WK+V + ELS + ++V I
Sbjct: 601 TVAEENLMWNDEEESRSCLMIDGEWKRVNDGELSGMASSVTI 609

BLAST of MC02g0141.1 vs. TAIR 10
Match: AT5G12220.2 (las1-like family protein )

HSP 1 Score: 364.8 bits (935), Expect = 1.4e-100
Identity = 254/642 (39.56%), Postives = 365/642 (56.85%), Query Frame = 0

Query: 1   MESLLG----FEEVPVENEEEQSSTYSRKLVPWSTWSEWLFVSQSIFSDSPDSVAAALRR 60
           ME+L G     E    E ++++SS    K VPW +W EW  V +S+FS SPD +A+AL R
Sbjct: 1   MEALAGIEADIESYFGEQDQQKSSPDGCKQVPWLSWEEWDSVRESLFSSSPDRIASALER 60

Query: 61  VSTWRSRGCLPVVVEVTAAIIEIQQKDPYFIKNQSLDASVDGKEDQLNNVSLSEEALAML 120
           V+TWRSRG LP  V+VT ++IEIQ KD +             +E Q  +   SE  L ML
Sbjct: 61  VATWRSRGSLPAPVDVTCSLIEIQLKDGFI-----------PREKQSADALYSEHLLQML 120

Query: 121 YCMAIMRLVNGVVEKTRKKTEVSIAVAADAIGLPRVLIDIRHEGSHRDLPALQVVRCAST 180
           YCM I+RLVN V+EKTR++ +VSIA AA AI           EGSHR+LPAL V+R A+ 
Sbjct: 121 YCMGILRLVNCVIEKTRRREDVSIADAARAI-----------EGSHRELPALSVLRDAAD 180

Query: 181 KALRWLRSYYWEPQEKAIPFQGDISTTIRKEIKSRLRDLAFNLKVKQNPSLGSAMVKPKR 240
           +AL WL+SYYWE Q+  IP + D + +IR+E+KS+LR LAF+L++K+NP   S +VK K 
Sbjct: 181 EALEWLKSYYWEQQKYQIPLKRDGTASIRREVKSKLRKLAFSLQLKRNPQFDSPLVKEKC 240

Query: 241 LKTKTGKTLKVLVQLYSSFSSEFVSVLLELLLKAMRSSDLMCSENSQKSRSTV-LDGWKL 300
              +  K +  LV+LY SFS+E  SVLLE LLKA+ SS     +N       V LD WK 
Sbjct: 241 SNKRIRKIVSSLVELYPSFSAEISSVLLEFLLKALDSSKSTDLQNKAGQDFRVFLDEWKP 300

Query: 301 VIAKISNKEPELLLELLEAVLEKIETREGLEYESQYFMSSDHRMESCQVSQLSSLFAWLV 360
           VI ++SN+EPELLL LL+ VL+ I+  E   +E++  +      E  QV Q+  LFAWLV
Sbjct: 301 VIMELSNREPELLLTLLKEVLDMIQNNERRRHETEKPVE-----EVSQVEQVPFLFAWLV 360

Query: 361 DKLKGLKLKRKGEVFYGKHIPKGILMEVLRKCLLASAPGNKRLMDSATDLAQLMGAASLV 420
             L G K  ++      K  P   LME++R+CL+  A G + ++ SA  LA+++G   L 
Sbjct: 361 SLLNGSKHFQRNSSLEVKP-PSIFLMELIRRCLVLGALGYELVLKSAFLLAEIVGGRVLK 420

Query: 421 KKLNKLSILNLPNLPNSHVPEGESPTQTTSDNSFNQVEGSFHEASQKLEAIKLH--KRKN 480
           +KL KL ++   +  ++ VP  +S T  T+  +  + E +   A ++LE +KL   K+K 
Sbjct: 421 EKLIKLPLM---HKSSAIVPLKQSSTLVTASTTLLEQEKNLGNAGKRLEFVKLQLSKKKG 480

Query: 481 LTNPVDGNVGSSSRWTIVSSWTPCPIGMLPRSIGSSGRLPVLDLNPESENTSEEPRSKEN 540
           +     GN     RW    +W+PCPIGMLPR IGSSGRLP+LD N  +++ S++ +   N
Sbjct: 481 IDTDKTGN-----RWRKAKTWSPCPIGMLPRIIGSSGRLPLLD-NQNAKSISKQAQGNNN 540

Query: 541 CELSNYSHKREASSDIQQLDSCSVKKLKE----------TNENFQAETE-DVESA----- 600
                   KR A  +  QL+    K+ ++          T E+++ E E ++E A     
Sbjct: 541 A-------KRGAECNRNQLEKSPCKRARKSAGDSESNDVTLESYEEEAEMEIEHAYEETE 598

Query: 601 ------------KGVKGRLLIGGIWKKVGEEELSAIQAAVRI 608
                       +  +  L+I G WK+V + ELS + ++V I
Sbjct: 601 TVAEENLMWNDEEESRSCLMIDGEWKRVNDGELSGMASSVTI 598