MC01g_new0440.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC01g_new0440.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncysteine-rich receptor-like protein kinase 10
LocationMC01: 18193841 .. 18195397 (+)
Sequence length801
RNA-Seq ExpressionMC01g_new0440.1
SyntenyMC01g_new0440.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGAAGACTGTGGACTGGTCTCACGACACGAAAATAGCAGTCTCAATTTTCCTCTCAGAATTTTCCAATGGAAGGTGGCGTGATTAAATACGGAACTCAACAAGACTATGCCAATTCCAAGTGGGTCGTAGCGTAGGTTCTCTCTTCCAATTTCTATATATGTTTTGATTCAAAAACCGACTGGATTTGATTTGACAGACCCAACAAAAATGGAGATCCCCACAACGCTTTTCTTCCTTATCTCCTTGGTTCTCTCACAGTTGATTTTGAGCACGGTTTCTCAGCCGGACTTTTTCTTTAGCAAATGCTCAGACAAATTTGGCAACTACAGCAACAGCAGTCCTTTCAGGGGAAATCTGAACAGCGCACTCGCCACCATCTCCTCCAAATCCAGCACTCAGGTAGTTGACTACGGCTTCAACAACGCCTCCTCCGGCGAAGATCCCGACAGAGCCAACGTTAAGGCGCTATGTCGTGGAGGCGTTTCCTTGGAACAATGCAGAATTTGCGTGAGTAACTCGGTCCGGAGGATTTTACAAAATTGCCCTTCCCAGAAGGAAGGCACTGGATGGTACGGGAATTGCCAGATTGTTTACTCAAACAACTCCGTCCATGGGGAGATATCCATTTCAGGGGCGACCCGCATTTTATACAACACCGGTAGAGCCCCAGACGCGAAGGGTTTCAATGAGGAACTGAGGGAGTTGCTGGATGGGCTGCGGGACAGGGCTGCGTCAGGGAGTTCTATTCGCAAGTCCGCCAGCGGAGATGTGAGGCTTGAAGCTCCGAATACGTATACCATATATGGGCTTGTTGATTGTTTTCCCGACATGTCGTTCTTCGACTGTGATGTGTGCCTCAGTCGGCTTCAGAGTAATCTTCCCAGTTGCTGTAGCGGCAGCATAGGGGCCAGATTGGTTGTCACTAGTTGTCAAATCAATTATGAGATCCACCCTCTGTATGAGTCACTCCTCTCTCCGCCCTCTCAATACGCGCCTCCAACAATTTAGCATTCCCATCATTTGTTGGGAGAAGAAAGAAAGTTGATTAAGTTCCAATGTTATTTGATGAATTCGATGCACTGCCAAACCCAAATCACTAGTTGGGATTTGGGAAGGAAGGTTAGATTAATTAGCTTTATCAATTTTTTTTTTATTTTTTATTTTATGAACTGCTCAGAGGAAAAGTTTTTTTAACTCGTCCAAATTTTACATGAATTCCGAGCGAGTTTTCTTCAAACTCATTCAATTTTAGCAAGATTCAAACACGATGTTTATCCATTTAATTTCAACTAACCCAAATTATAATAAAAACCAGGAGCCTGATATTTATGCAATATGGATTCAAGATCTATGTACATTTTAATTTCAACCAACCTAAATAATGTTAAAAAAATCATCCTCAACCCATATTTGAATCGAAAGAAAGTAGCAAAAATCAAAACCAAAAAATAGGAGGAGAAGAAAGAAAACTGTAAAACAAGAGAAGAGATCGAGAAAGAAAATGAAAAGAGACATAATAGTCCTTTCATATGTTGTAACGTTTTTTAAAAGTAC

mRNA sequence

ATGGAGATCCCCACAACGCTTTTCTTCCTTATCTCCTTGGTTCTCTCACAGTTGATTTTGAGCACGGTTTCTCAGCCGGACTTTTTCTTTAGCAAATGCTCAGACAAATTTGGCAACTACAGCAACAGCAGTCCTTTCAGGGGAAATCTGAACAGCGCACTCGCCACCATCTCCTCCAAATCCAGCACTCAGGTAGTTGACTACGGCTTCAACAACGCCTCCTCCGGCGAAGATCCCGACAGAGCCAACGTTAAGGCGCTATGTCGTGGAGGCGTTTCCTTGGAACAATGCAGAATTTGCGTGAGTAACTCGGTCCGGAGGATTTTACAAAATTGCCCTTCCCAGAAGGAAGGCACTGGATGGTACGGGAATTGCCAGATTGTTTACTCAAACAACTCCGTCCATGGGGAGATATCCATTTCAGGGGCGACCCGCATTTTATACAACACCGGTAGAGCCCCAGACGCGAAGGGTTTCAATGAGGAACTGAGGGAGTTGCTGGATGGGCTGCGGGACAGGGCTGCGTCAGGGAGTTCTATTCGCAAGTCCGCCAGCGGAGATGTGAGGCTTGAAGCTCCGAATACGTATACCATATATGGGCTTGTTGATTGTTTTCCCGACATGTCGTTCTTCGACTGTGATGTGTGCCTCAGTCGGCTTCAGAGTAATCTTCCCAGTTGCTGTAGCGGCAGCATAGGGGCCAGATTGGTTGTCACTAGTTGTCAAATCAATTATGAGATCCACCCTCTGTATGAGTCACTCCTCTCTCCGCCCTCTCAATACGCGCCTCCAACAATTTAG

Coding sequence (CDS)

ATGGAGATCCCCACAACGCTTTTCTTCCTTATCTCCTTGGTTCTCTCACAGTTGATTTTGAGCACGGTTTCTCAGCCGGACTTTTTCTTTAGCAAATGCTCAGACAAATTTGGCAACTACAGCAACAGCAGTCCTTTCAGGGGAAATCTGAACAGCGCACTCGCCACCATCTCCTCCAAATCCAGCACTCAGGTAGTTGACTACGGCTTCAACAACGCCTCCTCCGGCGAAGATCCCGACAGAGCCAACGTTAAGGCGCTATGTCGTGGAGGCGTTTCCTTGGAACAATGCAGAATTTGCGTGAGTAACTCGGTCCGGAGGATTTTACAAAATTGCCCTTCCCAGAAGGAAGGCACTGGATGGTACGGGAATTGCCAGATTGTTTACTCAAACAACTCCGTCCATGGGGAGATATCCATTTCAGGGGCGACCCGCATTTTATACAACACCGGTAGAGCCCCAGACGCGAAGGGTTTCAATGAGGAACTGAGGGAGTTGCTGGATGGGCTGCGGGACAGGGCTGCGTCAGGGAGTTCTATTCGCAAGTCCGCCAGCGGAGATGTGAGGCTTGAAGCTCCGAATACGTATACCATATATGGGCTTGTTGATTGTTTTCCCGACATGTCGTTCTTCGACTGTGATGTGTGCCTCAGTCGGCTTCAGAGTAATCTTCCCAGTTGCTGTAGCGGCAGCATAGGGGCCAGATTGGTTGTCACTAGTTGTCAAATCAATTATGAGATCCACCCTCTGTATGAGTCACTCCTCTCTCCGCCCTCTCAATACGCGCCTCCAACAATTTAG

Protein sequence

MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYEIHPLYESLLSPPSQYAPPTI
Homology
BLAST of MC01g_new0440.1 vs. ExPASy Swiss-Prot
Match: Q9LRJ9 (Cysteine-rich repeat secretory protein 38 OS=Arabidopsis thaliana OX=3702 GN=CRRSP38 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.3e-33
Identity = 81/247 (32.79%), Postives = 122/247 (49.39%), Query Frame = 0

Query: 7   LFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVV 66
           L   I ++    +LS      F F KCSD  G++++ S +  NLN+  + +S K    V 
Sbjct: 13  LAIAIQILSIHTVLSQSQNNAFLFHKCSDIEGSFTSKSLYESNLNNLFSQLSYK----VP 72

Query: 67  DYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQ 126
             GF  +S+G  P+  N  ALCRG  S   CR C+  ++  + Q CP+ K G  WY NC 
Sbjct: 73  STGFAASSTGNTPNNVNGLALCRGDASSSDCRSCLETAIPELRQRCPNNKAGIVWYDNCL 132

Query: 127 IVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRKSASG 186
           + YS+ +  G+I        LYN     D   FN + + LL  L  +A +  + +  A+G
Sbjct: 133 VKYSSTNFFGKIDFENRF-YLYNVKNVSDPSTFNSQTKALLTELTKKATTRDNQKLFATG 192

Query: 187 DVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYE 246
           +  +       +YGLV C  D+    C  CL+ +   LP+CC G  G R+V  SC   YE
Sbjct: 193 EKNI---GKNKLYGLVQCTRDLKSITCKACLNGIIGELPNCCDGKEGGRVVGGSCNFRYE 251

Query: 247 IHPLYES 254
           I+P  ++
Sbjct: 253 IYPFVKT 251

BLAST of MC01g_new0440.1 vs. ExPASy Swiss-Prot
Match: Q9M0X5 (Cysteine-rich receptor-like protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CRK25 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-30
Identity = 85/259 (32.82%), Postives = 130/259 (50.19%), Query Frame = 0

Query: 10  LISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVVDYG 69
           L S+ L  ++ +  S P + +  C +    YS +S +  NL + L+++SS ++       
Sbjct: 11  LFSVFLFMILKTVTSDPTYLYHICPNT-TTYSRNSSYLTNLRTVLSSLSSPNAAYA--SL 70

Query: 70  FNNASSGEDPDRANVKA--LCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQI 129
           F+NA++GE+ D   V    LCRG VS E CR CV+ +    LQ CP +K    WY  C +
Sbjct: 71  FDNAAAGEENDSNRVYGVFLCRGDVSAEICRDCVAFAANETLQRCPREKVAVIWYDECMV 130

Query: 130 VYSNNSVHGEISISGATRILYNTGRAPD-AKGFNEELRELLDGLRDRAASGSSIRKSASG 189
            YSN S+ G++ I     +        +    FNE L  LL  +  +AA  S  RK A+ 
Sbjct: 131 RYSNQSIVGQMRIRPGVFLTNKQNITENQVSRFNESLPALLIDVAVKAALSS--RKFATE 190

Query: 190 DVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYE 249
                     TIY LV C PD++  DC+ CL ++ + LP CC  S+G R++  SC   YE
Sbjct: 191 KANFTV--FQTIYSLVQCTPDLTNQDCESCLRQVINYLPRCCDRSVGGRVIAPSCSFRYE 250

Query: 250 IHPLYESLLSPPSQYAPPT 266
           ++P Y   ++      PP+
Sbjct: 251 LYPFYNETIAAAPMAPPPS 262

BLAST of MC01g_new0440.1 vs. ExPASy Swiss-Prot
Match: Q9T0J1 (Cysteine-rich receptor-like protein kinase 26 OS=Arabidopsis thaliana OX=3702 GN=CRK26 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-30
Identity = 86/257 (33.46%), Postives = 132/257 (51.36%), Query Frame = 0

Query: 10  LISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVVDYG 69
           LISL+      +  SQP      CS+  GN++ ++P+  NL+     ISS SS +    G
Sbjct: 8   LISLLFQIQCFTVKSQPVPLNQICSNVTGNFTVNTPYAVNLD---RLISSLSSLRRNVNG 67

Query: 70  FNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQIVY 129
           F N S G+  ++ N  + CRG V LE C  C++ + +R++  CP QKE   WY  C   Y
Sbjct: 68  FYNISVGDSDEKVNSISQCRGDVKLEVCINCIAMAGKRLVTLCPVQKEAIIWYDKCTFRY 127

Query: 130 SNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAA-SGSSIRKSASGDV 189
           SN ++   + IS  T I        D   + + LR LL+GL++RA+  G S +    G+ 
Sbjct: 128 SNRTIFNRLEISPHTSITGTRNFTGDRDSWEKSLRGLLEGLKNRASVIGRSKKNFVVGET 187

Query: 190 RLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYEIH 249
               P+  T++GLV C PD+S  DC  CLS+  + +PSCC   +G+ ++  SC + Y   
Sbjct: 188 --SGPSFQTLFGLVQCTPDISEEDCSYCLSQGIAKIPSCCDMKMGSYVMSPSCMLAYAPW 247

Query: 250 PLYESLLSPPSQYAPPT 266
             Y+ + +      P T
Sbjct: 248 RFYDPVDTDDPSSVPAT 259

BLAST of MC01g_new0440.1 vs. ExPASy Swiss-Prot
Match: Q9LRL8 (Putative cysteine-rich repeat secretory protein 21 OS=Arabidopsis thaliana OX=3702 GN=CRRSP21 PE=5 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 7.3e-27
Identity = 74/243 (30.45%), Postives = 122/243 (50.21%), Query Frame = 0

Query: 11  ISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVVDYGF 70
           + L+    +LS      +   KC +  G Y++ +P+  NLNS + TI    ST  +++GF
Sbjct: 20  LQLLFIPNVLSLNQTNAYLHHKCVNDQGTYNSGTPYEKNLNSLIRTI----STLNLNFGF 79

Query: 71  NNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQIVYS 130
            ++S+G+ P+   +K  CRG     +CR C++ +   I + CP  KE   WY NC +  S
Sbjct: 80  VHSSNGDAPNSVFIKLQCRGDSYFSKCRSCLATANSEIRRRCPKNKEKIIWYDNCVLEIS 139

Query: 131 NNSVHGEISISGATRILYNTGRAPDAKG----FNEELRELLDGLRDRAASGSSIRKSASG 190
           +     +I        +YN   A D  G    FN+  R+LL  L+++A   S  +K    
Sbjct: 140 SIDTFNKIDYQN-NFFMYN---AKDVSGDIELFNKNTRDLLHELKEKANIKS--KKDFMY 199

Query: 191 DVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYE 250
               +      +Y +V C  D++  +C+VCL+ + + LP CC G  G R++ TSC   YE
Sbjct: 200 AAGEKGLGEKKLYAMVQCTKDLTPNNCNVCLNWIMAKLPKCCKGKQGGRVLSTSCNFRYE 252

BLAST of MC01g_new0440.1 vs. ExPASy Swiss-Prot
Match: Q8GYA4 (Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN=CRK10 PE=1 SV=3)

HSP 1 Score: 120.9 bits (302), Expect = 2.1e-26
Identity = 86/267 (32.21%), Postives = 134/267 (50.19%), Query Frame = 0

Query: 1   MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK 60
           M   ++ FFL           +   P + +  C +   NY+++S +  NL + LA++SS+
Sbjct: 12  MSYYSSFFFLFLFSFLTSFRVSAQDPTYVYHTCQNT-ANYTSNSTYNNNLKTLLASLSSR 71

Query: 61  SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG 120
           +++     GF NA+ G+ PDR      CRG VS E CR CVS +V   L  CP+QKE T 
Sbjct: 72  NAS--YSTGFQNATVGQAPDRVTGLFNCRGDVSTEVCRRCVSFAVNDTLTRCPNQKEATL 131

Query: 121 WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI 180
           +Y  C + YSN ++   +  +G   IL NT      +   + L +L+    ++AA+ +  
Sbjct: 132 YYDECVLRYSNQNILSTLITTGGV-ILVNTRNVTSNQ--LDLLSDLVLPTLNQAATVALN 191

Query: 181 RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS 240
                G  +       + YGLV C PD++  DC  CL  + + +P   +  IGAR++  S
Sbjct: 192 SSKKFGTRKNNFTALQSFYGLVQCTPDLTRQDCSRCLQLVINQIP---TDRIGARIINPS 251

Query: 241 CQINYEIHPLY-ESLLSPPSQYAPPTI 267
           C   YEI+  Y ES + PP    PP+I
Sbjct: 252 CTSRYEIYAFYTESAVPPPP--PPPSI 267

BLAST of MC01g_new0440.1 vs. NCBI nr
Match: XP_022142358.1 (cysteine-rich repeat secretory protein 38-like [Momordica charantia])

HSP 1 Score: 531 bits (1369), Expect = 2.12e-190
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK 60
           MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK
Sbjct: 1   MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK 60

Query: 61  SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG 120
           SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG
Sbjct: 61  SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG 120

Query: 121 WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI 180
           WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI
Sbjct: 121 WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI 180

Query: 181 RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS 240
           RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS
Sbjct: 181 RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS 240

Query: 241 CQINYEIHPLYESLLSPPSQYAPPTI 266
           CQINYEIHPLYESLLSPPSQYAPPTI
Sbjct: 241 CQINYEIHPLYESLLSPPSQYAPPTI 266

BLAST of MC01g_new0440.1 vs. NCBI nr
Match: XP_022142245.1 (cysteine-rich repeat secretory protein 38-like [Momordica charantia])

HSP 1 Score: 413 bits (1062), Expect = 3.01e-143
Identity = 209/261 (80.08%), Postives = 232/261 (88.89%), Query Frame = 0

Query: 1   MEIPTTLFFLISLVL--SQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATIS 60
           MEIPTTLF LISLVL   QLILSTV +PDFFFSKCSDKFGNYSN+SPF+ NL++ALA+IS
Sbjct: 1   MEIPTTLFLLISLVLLLPQLILSTVYEPDFFFSKCSDKFGNYSNNSPFKKNLHNALASIS 60

Query: 61  SKSST--QVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQK 120
           S+SST  QV DYGF  ASSGE+PDRANVKALCRGGVSLE CRICV NSVRRILQ CPSQK
Sbjct: 61  SRSSTRTQVSDYGFYKASSGEEPDRANVKALCRGGVSLELCRICVRNSVRRILQTCPSQK 120

Query: 121 EGTGWYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAAS 180
           EG GWYG+CQIVYSNNS+ GEIS++GAT+ L++T RAPDA+GFNE LRELLDGLR++AAS
Sbjct: 121 EGAGWYGDCQIVYSNNSIDGEISVAGATQTLFSTNRAPDARGFNEALRELLDGLREKAAS 180

Query: 181 GSSIRKSASGDVRLEAPNT-YTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGAR 240
           GS IRKSASGDV+L+APNT YTIYGL DCFPDMS  DCDVCLSRLQS +P+CCSGS GAR
Sbjct: 181 GSCIRKSASGDVKLQAPNTTYTIYGLADCFPDMSSLDCDVCLSRLQSKIPNCCSGSTGAR 240

Query: 241 LVVTSCQINYEIHPLYESLLS 256
           L+ TSCQINYEIHPLY S LS
Sbjct: 241 LIATSCQINYEIHPLYYSFLS 261

BLAST of MC01g_new0440.1 vs. NCBI nr
Match: XP_022142208.1 (cysteine-rich receptor-like protein kinase 10 [Momordica charantia])

HSP 1 Score: 410 bits (1053), Expect = 4.59e-136
Identity = 202/256 (78.91%), Postives = 228/256 (89.06%), Query Frame = 0

Query: 5   TTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSST- 64
           TTLFFLIS +L+QLI +T SQP+FFF KCSD  GNY+N+SPF+ NL++ALA++SSKS+T 
Sbjct: 32  TTLFFLISFILAQLIFTTTSQPEFFFWKCSDN-GNYTNNSPFKRNLDNALASVSSKSNTN 91

Query: 65  -QVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWY 124
            Q  DYGF NASSGEDPDRANVK LCRGG  LEQCRICV+NSVRRILQNCP+QKEGTGWY
Sbjct: 92  PQGTDYGFYNASSGEDPDRANVKVLCRGGAPLEQCRICVNNSVRRILQNCPAQKEGTGWY 151

Query: 125 GNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRK 184
            +CQI+YSNNS++GEISI+GA +  YN   APDAKGFNE  RELLDGLR++AA GSSIRK
Sbjct: 152 HDCQILYSNNSINGEISIAGAGQTYYNVNTAPDAKGFNEARRELLDGLREKAARGSSIRK 211

Query: 185 SASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQ 244
           SA GDV+L+APNTYTIYGLVDCFPDM FFDCDVCLSRLQS+LPSCC+GSIGARLVVTSCQ
Sbjct: 212 SAGGDVKLQAPNTYTIYGLVDCFPDMPFFDCDVCLSRLQSSLPSCCNGSIGARLVVTSCQ 271

Query: 245 INYEIHPLYESLLSPP 258
           INYEIHPLYE LLSPP
Sbjct: 272 INYEIHPLYELLLSPP 286

BLAST of MC01g_new0440.1 vs. NCBI nr
Match: XP_023517770.1 (cysteine-rich receptor-like protein kinase 29 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369 bits (948), Expect = 6.22e-121
Identity = 181/251 (72.11%), Postives = 212/251 (84.46%), Query Frame = 0

Query: 9   FLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQV-VD 68
           F +S V++QL+L+T SQ DFF SKCS+   NY+N+SPF+ NL +ALA I+S S+T   VD
Sbjct: 12  FYLSFVIAQLMLTTTSQRDFFLSKCSEN-ANYTNNSPFKKNLENALALITSNSNTDTRVD 71

Query: 69  YGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQI 128
           YGF NA+ GE PD AN KALCR GV LE CR CV++S RRI+QNCP+QKEG GWY +CQI
Sbjct: 72  YGFYNATFGEAPDTANAKALCRKGVPLEDCRTCVNDSARRIVQNCPNQKEGAGWYHHCQI 131

Query: 129 VYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRKSASGD 188
           +YSNNSVHGEI+I+ A++I +NTG+APDA  FNEE RELLD LR+ AA GSSIRKSA GD
Sbjct: 132 LYSNNSVHGEITINSASQIAFNTGKAPDANSFNEERRELLDRLREEAALGSSIRKSAGGD 191

Query: 189 VRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYEI 248
           V+L  PNTYTIYGLVDCFPDMS+FDCDVCLSRLQSNLPSCC+GSIGARL+VTSCQINYEI
Sbjct: 192 VKLANPNTYTIYGLVDCFPDMSYFDCDVCLSRLQSNLPSCCNGSIGARLIVTSCQINYEI 251

Query: 249 HPLYESLLSPP 258
           HPLY +LLSPP
Sbjct: 252 HPLYAALLSPP 261

BLAST of MC01g_new0440.1 vs. NCBI nr
Match: XP_023517769.1 (cysteine-rich receptor-like protein kinase 29 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369 bits (948), Expect = 1.45e-120
Identity = 181/251 (72.11%), Postives = 212/251 (84.46%), Query Frame = 0

Query: 9   FLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQV-VD 68
           F +S V++QL+L+T SQ DFF SKCS+   NY+N+SPF+ NL +ALA I+S S+T   VD
Sbjct: 12  FYLSFVIAQLMLTTTSQRDFFLSKCSEN-ANYTNNSPFKKNLENALALITSNSNTDTRVD 71

Query: 69  YGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQI 128
           YGF NA+ GE PD AN KALCR GV LE CR CV++S RRI+QNCP+QKEG GWY +CQI
Sbjct: 72  YGFYNATFGEAPDTANAKALCRKGVPLEDCRTCVNDSARRIVQNCPNQKEGAGWYHHCQI 131

Query: 129 VYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRKSASGD 188
           +YSNNSVHGEI+I+ A++I +NTG+APDA  FNEE RELLD LR+ AA GSSIRKSA GD
Sbjct: 132 LYSNNSVHGEITINSASQIAFNTGKAPDANSFNEERRELLDRLREEAALGSSIRKSAGGD 191

Query: 189 VRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYEI 248
           V+L  PNTYTIYGLVDCFPDMS+FDCDVCLSRLQSNLPSCC+GSIGARL+VTSCQINYEI
Sbjct: 192 VKLANPNTYTIYGLVDCFPDMSYFDCDVCLSRLQSNLPSCCNGSIGARLIVTSCQINYEI 251

Query: 249 HPLYESLLSPP 258
           HPLY +LLSPP
Sbjct: 252 HPLYAALLSPP 261

BLAST of MC01g_new0440.1 vs. ExPASy TrEMBL
Match: A0A6J1CN27 (cysteine-rich repeat secretory protein 38-like OS=Momordica charantia OX=3673 GN=LOC111012497 PE=4 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 1.03e-190
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK 60
           MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK
Sbjct: 1   MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK 60

Query: 61  SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG 120
           SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG
Sbjct: 61  SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG 120

Query: 121 WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI 180
           WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI
Sbjct: 121 WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI 180

Query: 181 RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS 240
           RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS
Sbjct: 181 RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS 240

Query: 241 CQINYEIHPLYESLLSPPSQYAPPTI 266
           CQINYEIHPLYESLLSPPSQYAPPTI
Sbjct: 241 CQINYEIHPLYESLLSPPSQYAPPTI 266

BLAST of MC01g_new0440.1 vs. ExPASy TrEMBL
Match: A0A6J1CKD8 (cysteine-rich repeat secretory protein 38-like OS=Momordica charantia OX=3673 GN=LOC111012408 PE=4 SV=1)

HSP 1 Score: 413 bits (1062), Expect = 1.46e-143
Identity = 209/261 (80.08%), Postives = 232/261 (88.89%), Query Frame = 0

Query: 1   MEIPTTLFFLISLVL--SQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATIS 60
           MEIPTTLF LISLVL   QLILSTV +PDFFFSKCSDKFGNYSN+SPF+ NL++ALA+IS
Sbjct: 1   MEIPTTLFLLISLVLLLPQLILSTVYEPDFFFSKCSDKFGNYSNNSPFKKNLHNALASIS 60

Query: 61  SKSST--QVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQK 120
           S+SST  QV DYGF  ASSGE+PDRANVKALCRGGVSLE CRICV NSVRRILQ CPSQK
Sbjct: 61  SRSSTRTQVSDYGFYKASSGEEPDRANVKALCRGGVSLELCRICVRNSVRRILQTCPSQK 120

Query: 121 EGTGWYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAAS 180
           EG GWYG+CQIVYSNNS+ GEIS++GAT+ L++T RAPDA+GFNE LRELLDGLR++AAS
Sbjct: 121 EGAGWYGDCQIVYSNNSIDGEISVAGATQTLFSTNRAPDARGFNEALRELLDGLREKAAS 180

Query: 181 GSSIRKSASGDVRLEAPNT-YTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGAR 240
           GS IRKSASGDV+L+APNT YTIYGL DCFPDMS  DCDVCLSRLQS +P+CCSGS GAR
Sbjct: 181 GSCIRKSASGDVKLQAPNTTYTIYGLADCFPDMSSLDCDVCLSRLQSKIPNCCSGSTGAR 240

Query: 241 LVVTSCQINYEIHPLYESLLS 256
           L+ TSCQINYEIHPLY S LS
Sbjct: 241 LIATSCQINYEIHPLYYSFLS 261

BLAST of MC01g_new0440.1 vs. ExPASy TrEMBL
Match: A0A6J1CMQ0 (cysteine-rich receptor-like protein kinase 10 OS=Momordica charantia OX=3673 GN=LOC111012377 PE=4 SV=1)

HSP 1 Score: 410 bits (1053), Expect = 2.22e-136
Identity = 202/256 (78.91%), Postives = 228/256 (89.06%), Query Frame = 0

Query: 5   TTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSST- 64
           TTLFFLIS +L+QLI +T SQP+FFF KCSD  GNY+N+SPF+ NL++ALA++SSKS+T 
Sbjct: 32  TTLFFLISFILAQLIFTTTSQPEFFFWKCSDN-GNYTNNSPFKRNLDNALASVSSKSNTN 91

Query: 65  -QVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWY 124
            Q  DYGF NASSGEDPDRANVK LCRGG  LEQCRICV+NSVRRILQNCP+QKEGTGWY
Sbjct: 92  PQGTDYGFYNASSGEDPDRANVKVLCRGGAPLEQCRICVNNSVRRILQNCPAQKEGTGWY 151

Query: 125 GNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRK 184
            +CQI+YSNNS++GEISI+GA +  YN   APDAKGFNE  RELLDGLR++AA GSSIRK
Sbjct: 152 HDCQILYSNNSINGEISIAGAGQTYYNVNTAPDAKGFNEARRELLDGLREKAARGSSIRK 211

Query: 185 SASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQ 244
           SA GDV+L+APNTYTIYGLVDCFPDM FFDCDVCLSRLQS+LPSCC+GSIGARLVVTSCQ
Sbjct: 212 SAGGDVKLQAPNTYTIYGLVDCFPDMPFFDCDVCLSRLQSSLPSCCNGSIGARLVVTSCQ 271

Query: 245 INYEIHPLYESLLSPP 258
           INYEIHPLYE LLSPP
Sbjct: 272 INYEIHPLYELLLSPP 286

BLAST of MC01g_new0440.1 vs. ExPASy TrEMBL
Match: A0A6J1KV13 (putative receptor-like protein kinase At4g00960 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496690 PE=4 SV=1)

HSP 1 Score: 369 bits (946), Expect = 1.33e-120
Identity = 181/251 (72.11%), Postives = 212/251 (84.46%), Query Frame = 0

Query: 9   FLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQV-VD 68
           F +S V+++L+L+T SQ DFF  KCS+   NY+N+SPF+ NL +ALA+I+S S+T   VD
Sbjct: 12  FYLSFVIARLMLTTTSQRDFFLYKCSEN-ANYTNNSPFKKNLENALASITSNSNTDTRVD 71

Query: 69  YGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQI 128
           YGF NA+ GEDPD AN KALCR GV L+ CR CV++S RRILQNCP+QKEG GWY +CQI
Sbjct: 72  YGFYNATFGEDPDTANAKALCRKGVPLDDCRTCVNDSARRILQNCPNQKEGAGWYHHCQI 131

Query: 129 VYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRKSASGD 188
           +YSNNSVHGEI+I+ A +I +NTG+APDA  FNEE RELLD LR+ AA GSSIRKSA GD
Sbjct: 132 LYSNNSVHGEITINSAVQIAFNTGKAPDANTFNEERRELLDRLREEAALGSSIRKSAGGD 191

Query: 189 VRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYEI 248
           V+L  PNTYTIYGLVDCFPDMS+FDCDVCLSRLQSNLPSCC+GSIGARLVVTSCQINYEI
Sbjct: 192 VKLANPNTYTIYGLVDCFPDMSYFDCDVCLSRLQSNLPSCCNGSIGARLVVTSCQINYEI 251

Query: 249 HPLYESLLSPP 258
           HPLY +LLSPP
Sbjct: 252 HPLYAALLSPP 261

BLAST of MC01g_new0440.1 vs. ExPASy TrEMBL
Match: A0A6J1ELZ9 (cysteine-rich receptor-like protein kinase 29 OS=Cucurbita moschata OX=3662 GN=LOC111433774 PE=4 SV=1)

HSP 1 Score: 367 bits (942), Expect = 5.30e-120
Identity = 180/251 (71.71%), Postives = 212/251 (84.46%), Query Frame = 0

Query: 9   FLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQV-VD 68
           F +S V++QL+L+T SQ DFF SKCS+   NY+N+SPF+ NL +ALA+I+S S+T   VD
Sbjct: 12  FYLSFVIAQLMLTTTSQRDFFLSKCSEN-ANYTNNSPFKKNLENALASITSNSNTDTRVD 71

Query: 69  YGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQI 128
           YGF NA+ GE PD AN KALCR GV LE CR CV++S RRI+QNCP+QKEG GWY +CQI
Sbjct: 72  YGFYNATFGEAPDTANAKALCRKGVPLEDCRSCVNDSARRIVQNCPNQKEGAGWYHHCQI 131

Query: 129 VYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRKSASGD 188
           +YSNNSVHGEI+I+ A++I +NT +APDA  FNEE RELLD LR+ AA GSSIRKSA GD
Sbjct: 132 LYSNNSVHGEITINSASQIAFNTAQAPDANSFNEERRELLDRLREEAALGSSIRKSAGGD 191

Query: 189 VRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYEI 248
           V+L  PNTYTIYGLVDCFPDMS+FDCDVCLSRLQSNLPSCC+GSIGARL+VTSCQINYEI
Sbjct: 192 VKLANPNTYTIYGLVDCFPDMSYFDCDVCLSRLQSNLPSCCNGSIGARLIVTSCQINYEI 251

Query: 249 HPLYESLLSPP 258
           HPLY +LLSPP
Sbjct: 252 HPLYAALLSPP 261

BLAST of MC01g_new0440.1 vs. TAIR 10
Match: AT3G22060.1 (Receptor-like protein kinase-related family protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.7e-34
Identity = 81/247 (32.79%), Postives = 122/247 (49.39%), Query Frame = 0

Query: 7   LFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVV 66
           L   I ++    +LS      F F KCSD  G++++ S +  NLN+  + +S K    V 
Sbjct: 13  LAIAIQILSIHTVLSQSQNNAFLFHKCSDIEGSFTSKSLYESNLNNLFSQLSYK----VP 72

Query: 67  DYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQ 126
             GF  +S+G  P+  N  ALCRG  S   CR C+  ++  + Q CP+ K G  WY NC 
Sbjct: 73  STGFAASSTGNTPNNVNGLALCRGDASSSDCRSCLETAIPELRQRCPNNKAGIVWYDNCL 132

Query: 127 IVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSIRKSASG 186
           + YS+ +  G+I        LYN     D   FN + + LL  L  +A +  + +  A+G
Sbjct: 133 VKYSSTNFFGKIDFENRF-YLYNVKNVSDPSTFNSQTKALLTELTKKATTRDNQKLFATG 192

Query: 187 DVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYE 246
           +  +       +YGLV C  D+    C  CL+ +   LP+CC G  G R+V  SC   YE
Sbjct: 193 EKNI---GKNKLYGLVQCTRDLKSITCKACLNGIIGELPNCCDGKEGGRVVGGSCNFRYE 251

Query: 247 IHPLYES 254
           I+P  ++
Sbjct: 253 IYPFVKT 251

BLAST of MC01g_new0440.1 vs. TAIR 10
Match: AT4G05200.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 25 )

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-31
Identity = 85/259 (32.82%), Postives = 130/259 (50.19%), Query Frame = 0

Query: 10  LISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVVDYG 69
           L S+ L  ++ +  S P + +  C +    YS +S +  NL + L+++SS ++       
Sbjct: 11  LFSVFLFMILKTVTSDPTYLYHICPNT-TTYSRNSSYLTNLRTVLSSLSSPNAAYA--SL 70

Query: 70  FNNASSGEDPDRANVKA--LCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQI 129
           F+NA++GE+ D   V    LCRG VS E CR CV+ +    LQ CP +K    WY  C +
Sbjct: 71  FDNAAAGEENDSNRVYGVFLCRGDVSAEICRDCVAFAANETLQRCPREKVAVIWYDECMV 130

Query: 130 VYSNNSVHGEISISGATRILYNTGRAPD-AKGFNEELRELLDGLRDRAASGSSIRKSASG 189
            YSN S+ G++ I     +        +    FNE L  LL  +  +AA  S  RK A+ 
Sbjct: 131 RYSNQSIVGQMRIRPGVFLTNKQNITENQVSRFNESLPALLIDVAVKAALSS--RKFATE 190

Query: 190 DVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYE 249
                     TIY LV C PD++  DC+ CL ++ + LP CC  S+G R++  SC   YE
Sbjct: 191 KANFTV--FQTIYSLVQCTPDLTNQDCESCLRQVINYLPRCCDRSVGGRVIAPSCSFRYE 250

Query: 250 IHPLYESLLSPPSQYAPPT 266
           ++P Y   ++      PP+
Sbjct: 251 LYPFYNETIAAAPMAPPPS 262

BLAST of MC01g_new0440.1 vs. TAIR 10
Match: AT4G38830.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 26 )

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-31
Identity = 86/257 (33.46%), Postives = 132/257 (51.36%), Query Frame = 0

Query: 10  LISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSSTQVVDYG 69
           LISL+      +  SQP      CS+  GN++ ++P+  NL+     ISS SS +    G
Sbjct: 8   LISLLFQIQCFTVKSQPVPLNQICSNVTGNFTVNTPYAVNLD---RLISSLSSLRRNVNG 67

Query: 70  FNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWYGNCQIVY 129
           F N S G+  ++ N  + CRG V LE C  C++ + +R++  CP QKE   WY  C   Y
Sbjct: 68  FYNISVGDSDEKVNSISQCRGDVKLEVCINCIAMAGKRLVTLCPVQKEAIIWYDKCTFRY 127

Query: 130 SNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAA-SGSSIRKSASGDV 189
           SN ++   + IS  T I        D   + + LR LL+GL++RA+  G S +    G+ 
Sbjct: 128 SNRTIFNRLEISPHTSITGTRNFTGDRDSWEKSLRGLLEGLKNRASVIGRSKKNFVVGET 187

Query: 190 RLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTSCQINYEIH 249
               P+  T++GLV C PD+S  DC  CLS+  + +PSCC   +G+ ++  SC + Y   
Sbjct: 188 --SGPSFQTLFGLVQCTPDISEEDCSYCLSQGIAKIPSCCDMKMGSYVMSPSCMLAYAPW 247

Query: 250 PLYESLLSPPSQYAPPT 266
             Y+ + +      P T
Sbjct: 248 RFYDPVDTDDPSSVPAT 259

BLAST of MC01g_new0440.1 vs. TAIR 10
Match: AT4G23180.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 10 )

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-27
Identity = 86/267 (32.21%), Postives = 134/267 (50.19%), Query Frame = 0

Query: 1   MEIPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSK 60
           M   ++ FFL           +   P + +  C +   NY+++S +  NL + LA++SS+
Sbjct: 12  MSYYSSFFFLFLFSFLTSFRVSAQDPTYVYHTCQNT-ANYTSNSTYNNNLKTLLASLSSR 71

Query: 61  SSTQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTG 120
           +++     GF NA+ G+ PDR      CRG VS E CR CVS +V   L  CP+QKE T 
Sbjct: 72  NAS--YSTGFQNATVGQAPDRVTGLFNCRGDVSTEVCRRCVSFAVNDTLTRCPNQKEATL 131

Query: 121 WYGNCQIVYSNNSVHGEISISGATRILYNTGRAPDAKGFNEELRELLDGLRDRAASGSSI 180
           +Y  C + YSN ++   +  +G   IL NT      +   + L +L+    ++AA+ +  
Sbjct: 132 YYDECVLRYSNQNILSTLITTGGV-ILVNTRNVTSNQ--LDLLSDLVLPTLNQAATVALN 191

Query: 181 RKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVVTS 240
                G  +       + YGLV C PD++  DC  CL  + + +P   +  IGAR++  S
Sbjct: 192 SSKKFGTRKNNFTALQSFYGLVQCTPDLTRQDCSRCLQLVINQIP---TDRIGARIINPS 251

Query: 241 CQINYEIHPLY-ESLLSPPSQYAPPTI 267
           C   YEI+  Y ES + PP    PP+I
Sbjct: 252 CTSRYEIYAFYTESAVPPPP--PPPSI 267

BLAST of MC01g_new0440.1 vs. TAIR 10
Match: AT4G23140.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 6 )

HSP 1 Score: 115.5 bits (288), Expect = 6.4e-26
Identity = 89/267 (33.33%), Postives = 133/267 (49.81%), Query Frame = 0

Query: 3   IPTTLFFLISLVLSQLILSTVSQPDFFFSKCSDKFGNYSNSSPFRGNLNSALATISSKSS 62
           I     FL S + S    S  +Q  F+ +        YS++S +  NL + L+++SS+++
Sbjct: 5   ISFNFLFLFSFLTSSFTAS--AQDPFYLNHYCPNTTTYSSNSTYSTNLRTLLSSLSSRNA 64

Query: 63  TQVVDYGFNNASSGEDPDRANVKALCRGGVSLEQCRICVSNSVRRILQNCPSQKEGTGWY 122
           +     GF NA++G+ PDR     LCRG VS E CR CV+ SV + L  CP  +E   +Y
Sbjct: 65  S--YSTGFQNATAGKAPDRVTGLFLCRGDVSPEVCRNCVAFSVNQTLNLCPKVREAVFYY 124

Query: 123 GNCQIVYSNNSVHGEISISGATRILYNTGR-APDAK---GFNEELRELLDGLRDRAASGS 182
             C + YS+ ++      +    IL NT   +P+ K   GF   +   +     +AA+ S
Sbjct: 125 EQCILRYSHKNILSTAITNEGEFILSNTNTISPNQKQIDGFTSFVSSTMSEAAGKAANSS 184

Query: 183 SIRKSASGDVRLEAPNTYTIYGLVDCFPDMSFFDCDVCLSRLQSNLPSCCSGSIGARLVV 242
             RK  + +  L A     +YGL+ C PD++  D   CLS LQS++       IGARL  
Sbjct: 185 --RKLYTVNTELTA--YQNLYGLLQCTPDLTRAD---CLSCLQSSINGMALSRIGARLYW 244

Query: 243 TSCQINYEIHPLY-ESLLSPPSQYAPP 265
            SC   YE++P Y ES +  P    PP
Sbjct: 245 PSCTARYELYPFYNESAIETPPLPPPP 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LRJ92.3e-3332.79Cysteine-rich repeat secretory protein 38 OS=Arabidopsis thaliana OX=3702 GN=CRR... [more]
Q9M0X51.9e-3032.82Cysteine-rich receptor-like protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9T0J11.9e-3033.46Cysteine-rich receptor-like protein kinase 26 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9LRL87.3e-2730.45Putative cysteine-rich repeat secretory protein 21 OS=Arabidopsis thaliana OX=37... [more]
Q8GYA42.1e-2632.21Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
XP_022142358.12.12e-190100.00cysteine-rich repeat secretory protein 38-like [Momordica charantia][more]
XP_022142245.13.01e-14380.08cysteine-rich repeat secretory protein 38-like [Momordica charantia][more]
XP_022142208.14.59e-13678.91cysteine-rich receptor-like protein kinase 10 [Momordica charantia][more]
XP_023517770.16.22e-12172.11cysteine-rich receptor-like protein kinase 29 isoform X2 [Cucurbita pepo subsp. ... [more]
XP_023517769.11.45e-12072.11cysteine-rich receptor-like protein kinase 29 isoform X1 [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
A0A6J1CN271.03e-190100.00cysteine-rich repeat secretory protein 38-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1CKD81.46e-14380.08cysteine-rich repeat secretory protein 38-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1CMQ02.22e-13678.91cysteine-rich receptor-like protein kinase 10 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1KV131.33e-12072.11putative receptor-like protein kinase At4g00960 isoform X3 OS=Cucurbita maxima O... [more]
A0A6J1ELZ95.30e-12071.71cysteine-rich receptor-like protein kinase 29 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G22060.11.7e-3432.79Receptor-like protein kinase-related family protein [more]
AT4G05200.11.3e-3132.82cysteine-rich RLK (RECEPTOR-like protein kinase) 25 [more]
AT4G38830.11.3e-3133.46cysteine-rich RLK (RECEPTOR-like protein kinase) 26 [more]
AT4G23180.11.5e-2732.21cysteine-rich RLK (RECEPTOR-like protein kinase) 10 [more]
AT4G23140.16.4e-2633.33cysteine-rich RLK (RECEPTOR-like protein kinase) 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002902Gnk2-homologous domainPFAMPF01657Stress-antifungcoord: 33..131
e-value: 3.9E-15
score: 56.3
coord: 154..246
e-value: 4.4E-10
score: 40.1
IPR002902Gnk2-homologous domainPROSITEPS51473GNK2coord: 141..250
score: 17.982447
IPR002902Gnk2-homologous domainPROSITEPS51473GNK2coord: 28..134
score: 16.217287
IPR038408Gnk2-homologous domain superfamilyGENE3D3.30.430.20coord: 25..133
e-value: 3.0E-18
score: 67.9
coord: 142..249
e-value: 5.4E-21
score: 76.7
NoneNo IPR availablePANTHERPTHR32099:SF51CYSTEINE-RICH REPEAT SECRETORY PROTEIN 38-LIKEcoord: 13..258
NoneNo IPR availablePANTHERPTHR32099CYSTEINE-RICH REPEAT SECRETORY PROTEINcoord: 13..258

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC01g_new0440MC01g_new0440gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC01g_new0440.1-cdsMC01g_new0440.1-cds-MC01:18194051..18194851CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC01g_new0440.1MC01g_new0440.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity