MC01g1380.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC01g1380.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Aspergillus nuclease S(1)
Location	MC01: 18432570 .. 18436427 (+)
Sequence length	1133
RNA-Seq Expression	MC01g1380.1
Synteny	MC01g1380.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGCAGTCATGGGCCGGTTTGAGCTCTTCTGGAGAGCTTCCGTTTTTCTGCTACTTGTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGCAATCTGCAAGATAGCCGAGGTAAGAATATTCAGTTCATTTTTAAGCATCAGTCATCATTTATTTTGTCAAGCTTTCGGTGTTACAGAAGGGTAATAGGGAATATGGTGCAGTCCAGTTCAGAAAATAAGGATAATAACTGGGGTTGTTGATTGAAAGTCCTCGAAAGTGTAGCTTTAATAGTGTAGGTCAGGATTTAAGGTAAAGCAAACGTGGCAAAGGAGAGAGGACCTTGTGAAATTTGGTCCGCTATTAGAAAACTTTGGACCCATTTGGATATTTGACATATAGAACTGAAATAAACACTTGTATCCACTATTTTAGCTTCTTAGTAAGCTAGAAATCTGTTTGGTGAGACGACTTGGAGCTGGTTGTATCTGATATCATCCTATGATAGGGAGTGCAATCATTAGATCCTGCAGTGTTCAAGACAAGACTGATATCACTTTTCTGGTTTAGGGATATTTCACTGAAGATGCTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTCGCCGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCACTGGAGTAGCGTTTTACACTATGTTGACACTCCTGATTTCTTTTGTAATTATAATTACTCGAGTAAGTTTGAGCTTAAGGTATTTTCAGTTCCTTTTGTGAACTATCATGCCGTTTTACTGTTTTATATAATGGTTGCTATTGTAGTGTTTATGAGCTTTAAAATTCAAAAGCATTGTTGAACCACAGGAATACTGAGGCTGAGAAATTTGATAGTTCATGATAACATAACCAATGTATCCTCTGAAGTTAACTTATATGGCCAATGGCGTAATTATTATAGCACTAGCGCCAATATCTTCCTAAATTAAAAATTTCATATTCATAAGAACATAAGATTTTGACACAGAATTTCAATGTCTCGTTGCTTCAAGATGATAAAGTGTTCACATGAAATGAACTAACTTGTTCTGCAATTCTTGTGCCAGTTGCCTGTTGCCTTTTGTTCCCCTTCTAGCTTTGAAACATCTTATGTTGCCAACTACGAAGTTAATGAAAATATGTCATTCTGAACATGTCATATATGATGGTTATTAAATTCAATTTGATTTTTGATTCCTTTCATTCAACAAGGTATTTTTAGTTGCAAGATTTAACGCTAATGATTGATTTTCTCTTCATGACAATGTTTACGAGTGTAACAGTTTCCTCTGCTTCATTATAGGAGACTGTCATGACACTCATGGACATAACGGTAGGTGTGTGATTGCGGCAATTTACAACTATACCATGCAACTTGAATCAACTTACAAAGAGATGACTTCAGATGTTAGATGTAAGCACCGTAAATAACTACCCATCCTGTCCATACATTTTTCGAACAAACGTCATTTTTATTCACGTGGTATTTTCAGATAACTTAACAGAGGCTCTCATGTTCTTGTCCCATTTTATTGGAGATGTCCACCAGGTTTGCCCTTTCTACAGATCTGAACTTTTAGGCACTCAGAGAAACTGAAAATATGAAGTGCTAGGTTCATGAAACTTATATTATGTAACTACAAACTTTAGTCCTTGGAATATTGTTGAAAGTGTATTTTCATCCGATGGTAAAATCACTCTTTCTTTTCAAATAGGAATCTTTATAGTTTGATTAGCAAGTTTGTGCATTAACTACATAATAATACTAATAGTGTCAACTGTATTTCATTTTTTCCCTAAAGAATCGAATAATTTATGATTTTATTCCTTTGTATCTGAATTTTCCGTTATTTGGTGGCAGCCCCTGCATGTTGGTTTCATTGGAGATCTTGGTGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTTCATCATGTAAGTGCACCTAGAGATTTGAATTCCCTCCTCACAAGTACCCTCCTTATTTCATCACTATTCTTCATACACCTTTAACCATTTGACAGTAAACACACGAAACATCTCGTAAAACTTCACCATGATGGTTAAAACTACAAGCTCGGAAATCCCATTACATACTGCAGAATGTTTTGGTTTTTATCCATCTGGTATTGGAATAGTTTTGTCCATAAAAATGAAATGGTCTTTCTACAGGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACGTTCTACAATTCAAGCCTTGCACTAATGATTCAAGCTATTCAAAATAGCATTGTGGTGTGCCTCCTTTCTCTGATTTTATGTTCTGACTTGCCTTTTTTGATAGAACAATTTACTTTTCTTATTTTTTACTCATAGTTCTTTTTCCTTATGCCATCTTCGTTTTAAATTTCTGAAGGATGAATGGTATAATGATGTATCAAGTTGGAGAAATTGCACAGAGAACCACACAGTTTGTCCTAACCCGTGAGTATTTTATCATTCTTATCCCTTATCCATGTTCGGCAACCTGTTTATGTTCAAATCATGTAATCAAATGATTCCTCTGCTGTCATATGAGCATCCCTGATTAGTATACGAAGATGGACTTGCCACTATTATACGTTGCTGAAACTTAAGATAACGTCTTTTTTAGCTCGAAAGAGGAGAAAAATATTCATCGGGTGAAAAATAACCTTCATTTTCCCACCATAAAGTTCTAGGAAATGGTAAGACTTGATTGGATGCCTCTTATTTTTCAGGTATGCTTCTGAAAGTATTAGCTTGGCGTGTAAATATGCGTACAAGAATGCCACTCCCGGAAGCACACTAGATGGTATACTCTTTTTTCTAGACAACCTCTCCTACACTCACATGTATGGTTTCTTTTCCACATTAACTTATCTGTTTCCAGTGCTGTGGTTAAAAATAGGTTGTAGGTGAAACCTAAACTTCTTAATGGGGATTTTGGTGGAGGTGGGTATGAGCGTAATGAAAGGAGCGAGACCTCTAGATATCCAGTGATAAGAATGCGAAAGGGTGTAAACTGCATGGGATTTGATTCTATATGTCAAAACCAGATAGATGCCACTTAGTTTATTGTTGCAACTTGCAATCCTTACCGTTAAAAAGAGAGACATTTTCCAGTCATCAAGATCAGACGAATTTCTTGTGTTAAATCTTCTGTTTCTACATTATACTCGAGAGGTTTTAACCCATCCTGTTACCCATAGGAACGTTTCGAATACAAGTTCATCCCTGATTCCTTTCTGTTCTGCCAATATGATAAAAAAATCTGAATCATTAATCTGTTAAAGAAGGCTCCTGTTCAAAAAACTTTCGAGAAACTCTACCAGTTTGCTTGTTGAGGCATACAAAGATGCCAGAATAACTCAACTCTGCACTAGTTCTTTCTCTATGCTCCCAGCTTCCTGAATCTATAGAATTAAGAGTGGTTGGCTGACCAAATGAGCCAAAATTTCTCCTGTTTCACTGCAGACAGTTATTTTCTTAGCCGGTTGCCAGTCGTAGAGAAGAGGTTAGCACAAAGTGGCATCAGATTGGCTGCTACCCTCAACCGAATCGCGGCTTCTGAAGTGAAAGTGGCTGAAGCTTGAAGACAATGAATGGGAGAGATCAGATCCATTTCATACAATAGTAAAATGGTTGAGGACATGAATTGATTCATTCTCCATTCCTCTGTCCTGTACTATATTTATGATTGTTCATAAAGAACCCAATATGAGGAGAATCATCTGTTAATTGAACAGTCACTGGATAATATGATTCAGTTGATTCACTGCCTTTAGTCTGCATCTTAAAGATTAAATCCCAGATTGTAGTTCAG

mRNA sequence

AGAGCAGTCATGGGCCGGTTTGAGCTCTTCTGGAGAGCTTCCGTTTTTCTGCTACTTGTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGCAATCTGCAAGATAGCCGAGGGATATTTCACTGAAGATGCTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTCGCCGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCACTGGAGTAGCGTTTTACACTATGTTGACACTCCTGATTTCTTTTGTAATTATAATTACTCGAGAGACTGTCATGACACTCATGGACATAACGGTAGGTGTGTGATTGCGGCAATTTACAACTATACCATGCAACTTGAATCAACTTACAAAGAGATGACTTCAGATGTTAGATATAACTTAACAGAGGCTCTCATGTTCTTGTCCCATTTTATTGGAGATGTCCACCAGCCCCTGCATGTTGGTTTCATTGGAGATCTTGGTGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTTCATCATGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACGTTCTACAATTCAAGCCTTGCACTAATGATTCAAGCTATTCAAAATAGCATTGTGGATGAATGGTATAATGATGTATCAAGTTGGAGAAATTGCACAGAGAACCACACAGTTTGTCCTAACCCGTATGCTTCTGAAAGTATTAGCTTGGCGTGTAAATATGCGTACAAGAATGCCACTCCCGGAAGCACACTAGATGACAGTTATTTTCTTAGCCGGTTGCCAGTCGTAGAGAAGAGGTTAGCACAAAGTGGCATCAGATTGGCTGCTACCCTCAACCGAATCGCGGCTTCTGAAGTGAAAGTGGCTGAAGCTTGAAGACAATGAATGGGAGAGATCAGATCCATTTCATACAATAGTAAAATGGTTGAGGACATGAATTGATTCATTCTCCATTCCTCTGTCCTGTACTATATTTATGATTGTTCATAAAGAACCCAATATGAGGAGAATCATCTGTTAATTGAACAGTCACTGGATAATATGATTCAGTTGATTCACTGCCTTTAGTCTGCATCTTAAAGATTAAATCCCAGATTGTAGTTCAG

Coding sequence (CDS)

AGAGCAGTCATGGGCCGGTTTGAGCTCTTCTGGAGAGCTTCCGTTTTTCTGCTACTTGTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGCAATCTGCAAGATAGCCGAGGGATATTTCACTGAAGATGCTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTCGCCGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCACTGGAGTAGCGTTTTACACTATGTTGACACTCCTGATTTCTTTTGTAATTATAATTACTCGAGAGACTGTCATGACACTCATGGACATAACGGTAGGTGTGTGATTGCGGCAATTTACAACTATACCATGCAACTTGAATCAACTTACAAAGAGATGACTTCAGATGTTAGATATAACTTAACAGAGGCTCTCATGTTCTTGTCCCATTTTATTGGAGATGTCCACCAGCCCCTGCATGTTGGTTTCATTGGAGATCTTGGTGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTTCATCATGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACGTTCTACAATTCAAGCCTTGCACTAATGATTCAAGCTATTCAAAATAGCATTGTGGATGAATGGTATAATGATGTATCAAGTTGGAGAAATTGCACAGAGAACCACACAGTTTGTCCTAACCCGTATGCTTCTGAAAGTATTAGCTTGGCGTGTAAATATGCGTACAAGAATGCCACTCCCGGAAGCACACTAGATGACAGTTATTTTCTTAGCCGGTTGCCAGTCGTAGAGAAGAGGTTAGCACAAAGTGGCATCAGATTGGCTGCTACCCTCAACCGAATCGCGGCTTCTGAAGTGAAAGTGGCTGAAGCTTGA

Protein sequence

RAVMGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA

Homology

[Close]

BLAST of MC01g1380.1 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.4e-119
Identity = 200/289 (69.20%), Postives = 233/289 (80.62%), Query Frame = 0


Query: 10  FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWP 69
           F R  V   L+ G L WGKEGHY +CKIAE YF E+ ++ VK+LLP SA+GDLA+VCSWP
Sbjct: 9   FARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWP 68

Query: 70  DEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQLESTYK 129
           DEI+    + W+S LHYVDTPD+ CNY Y RDCHDTH +  RCV  AI+NYTMQL S  +
Sbjct: 69  DEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASE 128

Query: 130 EMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNMI 189
              + V YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN+ITVRWYRRKTNLHHVWDNMI
Sbjct: 129 NSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMI 188

Query: 190 IESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESISLACKY 249
           IESALKT+YN SL LMI+A+Q ++ ++W NDV  W +C  N T CPNPYASESI+LACKY
Sbjct: 189 IESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESINLACKY 248

Query: 250 AYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVA 299
           AY+NATPG+TL D YFLSRLP+VEKRLAQ GIRLAATLNRI +S+ K A
Sbjct: 249 AYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

[Close]

BLAST of MC01g1380.1 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.9e-108
Identity = 184/291 (63.23%), Postives = 226/291 (77.66%), Query Frame = 0


Query: 11  WRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAE-GDLAAVCS 70
           W  SV +L  LV G L WGK+GHY +CK+AEG+F +D ++ VK+LLP S + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 71  WPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQLEST 130
           WPDEI++ + + W+S LHYV+TP++ CNY Y RDCHDTH H   CV  AI+NYT QL S 
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSA 123

Query: 131 YKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDN 190
            +   + V YNLTEAL+FLSH++GDVHQPLH GF+GDLGGN+I V WY  K+NLHHVWDN
Sbjct: 124 SENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDN 183

Query: 191 MIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESISLAC 250
           MII+SAL+T+YNSSL  MIQA+Q  + + W NDV SW++C  +   CPN YASESI LAC
Sbjct: 184 MIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLAC 243

Query: 251 KYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVA 299
           KYAY+NATPG+TL D YFLSRLPVVEKRLAQ GIRLAATLNRI +++ K+A
Sbjct: 244 KYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

[Close]

BLAST of MC01g1380.1 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 7.5e-105
Identity = 186/299 (62.21%), Postives = 220/299 (73.58%), Query Frame = 0


Query: 4   MGRFELFWRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG     W  S+ +L  LV G L WG  GHYA+CKIA+ YF ED +  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEI++   + W+S LH+ DTPD+ CNY YSRDC         CV  AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
            QL ST +   S V YNLTEALMFLSH++GD+HQPLH GFIGDLGGN I V WY ++TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           H VWD+MIIESAL+T+YNSSL  MI  +Q  + + W NDV SW +C  N T CPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA 301
           SI LACKYAY+NAT G+TL D YF+SRLPVVEKRLAQ GIRLA TLNRI +++ K+A A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLARA 294

[Close]

BLAST of MC01g1380.1 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.3e-88
Identity = 157/274 (57.30%), Postives = 190/274 (69.34%), Query Frame = 0


Query: 17  LLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWPDEIRRKA 76
           L   P I GWGKEGH  ICKIA+    E A   VKELLP SAEGDL+++C W D +  K 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRV--KF 78

Query: 77  HYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQLESTYKEMTSDVR 136
            YHWSS LHY++TPD  C+Y Y+RDC D  G  GRCV  AIYNYT QL S     +S  +
Sbjct: 79  RYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 137 YNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKT 196
           YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ IIE+A   
Sbjct: 139 YNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEAD 198

Query: 197 FYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESISLACKYAYKNATP 256
            YNS+L  M+ A++ +I  EW + V  W  CT+  T CP+ YASE I  AC +AYK  T 
Sbjct: 199 LYNSALEGMVDALKKNITTEWADQVKRWETCTKK-TACPDIYASEGIQAACDWAYKGVTE 258

Query: 257 GSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRI 291
           G TL+D YF SRLP+V +RLAQ G+RLAATLNRI
Sbjct: 259 GDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRI 288

[Close]

BLAST of MC01g1380.1 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 8.1e-83
Identity = 150/298 (50.34%), Postives = 199/298 (66.78%), Query Frame = 0


Query: 11  WRASVFLLLVPGIL---------GWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 70
           +R+S  L+LV GIL          W KEGH   C+IA+         +V+ LLP   +GD
Sbjct: 5   FRSSTRLILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGD 64

Query: 71  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 130
           L+A+C WPD+IR    Y W+S LHY+DTPD  C+Y YSRDCHD HG    CV  AI N+T
Sbjct: 65  LSALCVWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFT 124

Query: 131 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 190
            QL+  Y E TSD RYN+TEAL+FLSHF+GD+HQP+HVGF  D GGN+I +RWY+ K+NL
Sbjct: 125 SQLQH-YGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNL 184

Query: 191 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDE-WYNDVSSWRNCTENHTVCPNPYAS 250
           HHVWD  II +ALK  Y+ +L L+ + ++ +I +  W++D+SSW  C +    CP+ YAS
Sbjct: 185 HHVWDREIILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECND-LIACPHKYAS 244

Query: 251 ESISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVA 299
           ESI LACK+ YK    G TL + YF +RLP+V KR+ Q G+RLA  LNR+ + +  +A
Sbjct: 245 ESIKLACKWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDDHAIA 300

[Close]

BLAST of MC01g1380.1 vs. NCBI nr
Match: XP_022142132.1 (endonuclease 4-like [Momordica charantia])

HSP 1 Score: 620 bits (1598), Expect = 3.24e-224
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 4   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 63
           MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA
Sbjct: 1   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 60

Query: 64  AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQ 123
           AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQ
Sbjct: 61  AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQ 120

Query: 124 LESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHH 183
           LESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHH
Sbjct: 121 LESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHH 180

Query: 184 VWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESI 243
           VWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESI
Sbjct: 181 VWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESI 240

Query: 244 SLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA 300
           SLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA
Sbjct: 241 SLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA 297

[Close]

BLAST of MC01g1380.1 vs. NCBI nr
Match: XP_038883252.1 (endonuclease 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 558 bits (1437), Expect = 1.22e-199
Identity = 265/298 (88.93%), Postives = 279/298 (93.62%), Query Frame = 0


Query: 4   MGRFELFWRAS--VFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG+ +L W A+  VFLLL+PGILGWGKEGHY ICKIAEGYFTED LS VKELLPASAEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEI+RKAHY WSS LHYVDTPDFFCNYN SRDCHDTHGH GRCV AAIYNYT
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
           MQLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWYRRKTNL
Sbjct: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           HHVWDNMIIESALKTFY+S+L LMIQAIQN+I DEWYNDVS+WRNCT NHT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAE 299
           SISLACKYAYKNATPGSTL+DSYFLSRLPVVEKRLAQSGIRLA+TLNRI ASEVKVAE
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAE 298

[Close]

BLAST of MC01g1380.1 vs. NCBI nr
Match: XP_038883248.1 (endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883250.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883251.1 endonuclease 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 549 bits (1414), Expect = 4.88e-196
Identity = 264/304 (86.84%), Postives = 278/304 (91.45%), Query Frame = 0


Query: 4   MGRFELFWRAS--VFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG+ +L W A+  VFLLL+PGILGWGKEGHY ICKIAEGYFTED LS VKELLPASAEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSR------DCHDTHGHNGRCVIA 123
           LAAVCSWPDEI+RKAHY WSS LHYVDTPDFFCNYN S       DCHDTHGH GRCV A
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSSKLKLYGDCHDTHGHKGRCVTA 120

Query: 124 AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY 183
           AIYNYTMQLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 184 RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCP 243
           RRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQN+I DEWYNDVS+WRNCT NHT CP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACP 240

Query: 244 NPYASESISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEV 299
           NPYASESISLACKYAYKNATPGSTL+DSYFLSRLPVVEKRLAQSGIRLA+TLNRI ASEV
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEV 300

[Close]

BLAST of MC01g1380.1 vs. NCBI nr
Match: XP_023543506.1 (endonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548 bits (1412), Expect = 7.89e-196
Identity = 262/298 (87.92%), Postives = 274/298 (91.95%), Query Frame = 0


Query: 4   MGRFEL--FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG+ EL    +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEIR KAHY WSS LHYVDTPDFFCNYNYSRDCHDTHGH GRCV AAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
            QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           HHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EWYNDVS+WRNC  NHT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAE 299
           SISLACKYAYKNATPGSTL+DSYFLSRLP+VEKRLAQSGIRLA+TLNRI ASEVKVAE
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAE 298

[Close]

BLAST of MC01g1380.1 vs. NCBI nr
Match: XP_022950165.1 (endonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 548 bits (1412), Expect = 7.89e-196
Identity = 261/298 (87.58%), Postives = 274/298 (91.95%), Query Frame = 0


Query: 4   MGRFEL--FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG+FEL    +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEIR KAHY WSS LHYVDTPDFFCNYNYSRDCHDTHGH GRCV AAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
            QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           HHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EWYNDVS+WRNC  NHT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAE 299
           SI LACKYAY+NATPGSTL+DSYFLSRLP+VEKRLAQSGIRLA+TLNRI ASEVKVAE
Sbjct: 241 SIRLACKYAYRNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAE 298

[Close]

BLAST of MC01g1380.1 vs. ExPASy TrEMBL
Match: A0A6J1CLU4 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV=1)

HSP 1 Score: 620 bits (1598), Expect = 1.57e-224
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 4   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 63
           MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA
Sbjct: 1   MGRFELFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLA 60

Query: 64  AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQ 123
           AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQ
Sbjct: 61  AVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQ 120

Query: 124 LESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHH 183
           LESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHH
Sbjct: 121 LESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHH 180

Query: 184 VWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESI 243
           VWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESI
Sbjct: 181 VWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESI 240

Query: 244 SLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA 300
           SLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA
Sbjct: 241 SLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA 297

[Close]

BLAST of MC01g1380.1 vs. ExPASy TrEMBL
Match: A0A6J1GEX6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 3.82e-196
Identity = 261/298 (87.58%), Postives = 274/298 (91.95%), Query Frame = 0


Query: 4   MGRFEL--FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG+FEL    +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEIR KAHY WSS LHYVDTPDFFCNYNYSRDCHDTHGH GRCV AAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
            QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           HHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EWYNDVS+WRNC  NHT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAE 299
           SI LACKYAY+NATPGSTL+DSYFLSRLP+VEKRLAQSGIRLA+TLNRI ASEVKVAE
Sbjct: 241 SIRLACKYAYRNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAE 298

[Close]

BLAST of MC01g1380.1 vs. ExPASy TrEMBL
Match: A0A6J1IVA4 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1)

HSP 1 Score: 545 bits (1404), Expect = 6.33e-195
Identity = 261/298 (87.58%), Postives = 273/298 (91.61%), Query Frame = 0


Query: 4   MGRFEL--FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG+ EL    +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEIR KAHY WSS LHYVDTPDFFCNYNYSRDCHDTH H GRCV AAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHEHKGRCVTAAIYNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
            QLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           HHVWDNMIIESALKTFY+S+L LMIQAIQ +I  EWYNDVS+WRNC  NHT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAE 299
           SISLACKYAYKNATPGSTL+DSYFLSRLP+VEKRLAQSGIRLA+TLNRI ASEVKVAE
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAE 298

[Close]

BLAST of MC01g1380.1 vs. ExPASy TrEMBL
Match: A0A1S3CF84 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 4.36e-186
Identity = 248/298 (83.22%), Postives = 271/298 (90.94%), Query Frame = 0


Query: 4   MGRFELFWRASVF--LLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG+ EL W A+VF  LLL+PGILGWG+EGHYA+CKIAEGY TEDALSMV+ELLPASAEGD
Sbjct: 1   MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSW DE+R    YHWSSVLH+VDTPDFFCNYN SRDCHD+H H GRCV AAIYNYT
Sbjct: 61  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
           MQLES YKE+TS+VRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGN I V WY+R TNL
Sbjct: 121 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRSTNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           HHVWD MIIESALK FY+S+L LMIQAIQ++I DEW+N+VS+WRNCT NHT CPNPYASE
Sbjct: 181 HHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAE 299
           SISLACKYAYKNATPGSTL+DSYFL+RLPVVEKRLAQSGIRLA+TLNRI ASEVKVAE
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVAE 298

[Close]

BLAST of MC01g1380.1 vs. ExPASy TrEMBL
Match: A0A1S3CEU3 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 523 bits (1348), Expect = 4.49e-186
Identity = 249/301 (82.72%), Postives = 273/301 (90.70%), Query Frame = 0


Query: 1   RAVMGRFELFWRASVF--LLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASA 60
           +A MG+ EL W A+VF  LLL+PGILGWG+EGHYA+CKIAEGY TEDALSMV+ELLPASA
Sbjct: 18  QADMGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASA 77

Query: 61  EGDLAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIY 120
           EGDLAAVCSW DE+R    YHWSSVLH+VDTPDFFCNYN SRDCHD+H H GRCV AAIY
Sbjct: 78  EGDLAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIY 137

Query: 121 NYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRK 180
           NYTMQLES YKE+TS+VRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGN I V WY+R 
Sbjct: 138 NYTMQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRS 197

Query: 181 TNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPY 240
           TNLHHVWD MIIESALK FY+S+L LMIQAIQ++I DEW+N+VS+WRNCT NHT CPNPY
Sbjct: 198 TNLHHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPY 257

Query: 241 ASESISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVA 299
           ASESISLACKYAYKNATPGSTL+DSYFL+RLPVVEKRLAQSGIRLA+TLNRI ASEVKVA
Sbjct: 258 ASESISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 317

[Close]

BLAST of MC01g1380.1 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 430.6 bits (1106), Expect = 1.0e-120
Identity = 200/289 (69.20%), Postives = 233/289 (80.62%), Query Frame = 0


Query: 10  FWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWP 69
           F R  V   L+ G L WGKEGHY +CKIAE YF E+ ++ VK+LLP SA+GDLA+VCSWP
Sbjct: 9   FARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWP 68

Query: 70  DEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQLESTYK 129
           DEI+    + W+S LHYVDTPD+ CNY Y RDCHDTH +  RCV  AI+NYTMQL S  +
Sbjct: 69  DEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASE 128

Query: 130 EMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNMI 189
              + V YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN+ITVRWYRRKTNLHHVWDNMI
Sbjct: 129 NSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMI 188

Query: 190 IESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESISLACKY 249
           IESALKT+YN SL LMI+A+Q ++ ++W NDV  W +C  N T CPNPYASESI+LACKY
Sbjct: 189 IESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESINLACKY 248

Query: 250 AYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVA 299
           AY+NATPG+TL D YFLSRLP+VEKRLAQ GIRLAATLNRI +S+ K A
Sbjct: 249 AYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

[Close]

BLAST of MC01g1380.1 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 393.7 bits (1010), Expect = 1.4e-109
Identity = 184/291 (63.23%), Postives = 226/291 (77.66%), Query Frame = 0


Query: 11  WRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAE-GDLAAVCS 70
           W  SV +L  LV G L WGK+GHY +CK+AEG+F +D ++ VK+LLP S + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 71  WPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQLEST 130
           WPDEI++ + + W+S LHYV+TP++ CNY Y RDCHDTH H   CV  AI+NYT QL S 
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSA 123

Query: 131 YKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDN 190
            +   + V YNLTEAL+FLSH++GDVHQPLH GF+GDLGGN+I V WY  K+NLHHVWDN
Sbjct: 124 SENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDN 183

Query: 191 MIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESISLAC 250
           MII+SAL+T+YNSSL  MIQA+Q  + + W NDV SW++C  +   CPN YASESI LAC
Sbjct: 184 MIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLAC 243

Query: 251 KYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVA 299
           KYAY+NATPG+TL D YFLSRLPVVEKRLAQ GIRLAATLNRI +++ K+A
Sbjct: 244 KYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

[Close]

BLAST of MC01g1380.1 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 381.7 bits (979), Expect = 5.4e-106
Identity = 186/299 (62.21%), Postives = 220/299 (73.58%), Query Frame = 0


Query: 4   MGRFELFWRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG     W  S+ +L  LV G L WG  GHYA+CKIA+ YF ED +  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEI++   + W+S LH+ DTPD+ CNY YSRDC         CV  AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
            QL ST +   S V YNLTEALMFLSH++GD+HQPLH GFIGDLGGN I V WY ++TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           H VWD+MIIESAL+T+YNSSL  MI  +Q  + + W NDV SW +C  N T CPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA 301
           SI LACKYAY+NAT G+TL D YF+SRLPVVEKRLAQ GIRLA TLNRI +++ K+A A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLARA 294

[Close]

BLAST of MC01g1380.1 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 381.7 bits (979), Expect = 5.4e-106
Identity = 186/299 (62.21%), Postives = 220/299 (73.58%), Query Frame = 0


Query: 4   MGRFELFWRASVFLL--LVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 63
           MG     W  S+ +L  LV G L WG  GHYA+CKIA+ YF ED +  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 64  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 123
           LAAVCSWPDEI++   + W+S LH+ DTPD+ CNY YSRDC         CV  AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 124 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 183
            QL ST +   S V YNLTEALMFLSH++GD+HQPLH GFIGDLGGN I V WY ++TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 184 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 243
           H VWD+MIIESAL+T+YNSSL  MI  +Q  + + W NDV SW +C  N T CPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 244 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAEA 301
           SI LACKYAY+NAT G+TL D YF+SRLPVVEKRLAQ GIRLA TLNRI +++ K+A A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLARA 294

[Close]

BLAST of MC01g1380.1 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 327.8 bits (839), Expect = 9.2e-90
Identity = 157/274 (57.30%), Postives = 190/274 (69.34%), Query Frame = 0


Query: 17  LLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGDLAAVCSWPDEIRRKA 76
           L   P I GWGKEGH  ICKIA+    E A   VKELLP SAEGDL+++C W D +  K 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRV--KF 78

Query: 77  HYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYTMQLESTYKEMTSDVR 136
            YHWSS LHY++TPD  C+Y Y+RDC D  G  GRCV  AIYNYT QL S     +S  +
Sbjct: 79  RYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 137 YNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKT 196
           YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ IIE+A   
Sbjct: 139 YNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEAD 198

Query: 197 FYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASESISLACKYAYKNATP 256
            YNS+L  M+ A++ +I  EW + V  W  CT+  T CP+ YASE I  AC +AYK  T 
Sbjct: 199 LYNSALEGMVDALKKNITTEWADQVKRWETCTKK-TACPDIYASEGIQAACDWAYKGVTE 258

Query: 257 GSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRI 291
           G TL+D YF SRLP+V +RLAQ G+RLAATLNRI
Sbjct: 259 GDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRI 288

The following BLAST results are available for this feature:

BLAST of MC01g1380.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJL0	1.4e-119	69.20	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
F4JJL3	1.9e-108	63.23	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	7.5e-105	62.21	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]
Q9C9G4	1.3e-88	57.30	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
Q9SXA6	8.1e-83	50.34	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]

BLAST of MC01g1380.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142132.1	3.24e-224	100.00	endonuclease 4-like [Momordica charantia]	[more]
XP_038883252.1	1.22e-199	88.93	endonuclease 4-like isoform X2 [Benincasa hispida]	[more]
XP_038883248.1	4.88e-196	86.84	endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease ...	[more]
XP_023543506.1	7.89e-196	87.92	endonuclease 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022950165.1	7.89e-196	87.58	endonuclease 4-like [Cucurbita moschata]	[more]

BLAST of MC01g1380.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLU4	1.57e-224	100.00	Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV...	[more]
A0A6J1GEX6	3.82e-196	87.58	Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=...	[more]
A0A6J1IVA4	6.33e-195	87.58	Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1	[more]
A0A1S3CF84	4.36e-186	83.22	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1	[more]
A0A1S3CEU3	4.49e-186	82.72	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1	[more]

BLAST of MC01g1380.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21585.1	1.0e-120	69.20	endonuclease 4	[more]
AT4G21600.1	1.4e-109	63.23	endonuclease 5	[more]
AT4G21590.1	5.4e-106	62.21	endonuclease 3	[more]
AT4G21590.2	5.4e-106	62.21	endonuclease 3	[more]
AT1G68290.1	9.2e-90	57.30	endonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 26..296 e-value: 8.1E-103 score: 345.9
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 26..290
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 26..291 e-value: 4.6E-80 score: 269.1
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 11..298
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 26..290 e-value: 4.20055E-86 score: 256.543
None	No IPR available	PANTHER	PTHR33146:SF23	ENDONUCLEASE 5	coord: 11..298

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC01g1380	MC01g1380	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18432570..18432686	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18433125..18433305	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18433907..18434017	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18434097..18434152	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18434465..18434548	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18434786..18434878	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18435000..18435067	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18435343..18435416	CDS
MC01g1380.1-cds	MC01g1380.1-cds-MC01:18436079..18436197	CDS

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC01g1380.1-utr3	MC01g1380.1-utr3-MC01:18436198..18436427	utr3

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC01g1380.1	MC01g1380.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity