MC00g_new0425.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC00g_new0425.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	scaffold7: 183887 .. 184981 (+)
Sequence length	420
RNA-Seq Expression	MC00g_new0425.1
Synteny	MC00g_new0425.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACATTCCTAGACTGAATGGGGCTAATTTTAAGGACTGGAAAGAAGACATCCAGATAGTACTTGGGTGTATGGATTTAGATCTTGCATTAACGGTAGACCGTCCTACTTCAACTAAGGAAAATCCTAATAAGGTTGAAATTGAGAAGTGGGATAGGTCTAATCGCATGTGTCTAATGATCATGAAGCGTTCAATTCCAGAAACATTTAGAGGCTCTATTGTTGAGGGAACGAATGCCAAAGGCTTTCTAAAGGAAATAGAGCAGTACTTTACCAAAAACGATAAGGCAGAGGCGAGTACCCTTATGGCAAAACTCACCTCTTGAAGATACGTTGGTAAAGGAAACATAAGGGAATACATAATGCAAATCTCAAATGTTGCAACAAAACGTAAGGCACTGAAGTTGGAAGTTTCTGAAGACTTTTTAGTGCACTTAGTTTTGAATTCTCTTCCAGCAGAGTATAGCCACTTCAGGGTGCGTTACAACACTCAGAAGGATAAATGGTCCCTGAATGAGCTAATCTCTCACTGTGTTCAAGAGGAAGAGAGGATGCAGCGAGAGAAGACAGAAAGTGTTCACATGGCTTCTACCTCAAAGAGTGTAAAGAGAAAGAGAGTGAATAATGCTGCGGAATCTTCTAAGCAGAAAAAGGAAAAGAAACAGGATTCAGGACCTGCTTGTTTCTTCTGTAAGAAGACTGGGCACATGAAAAAACAATGTGCCAAATATACTGCATGGCTATTGAAGAAGGGTATGTTTCTCTCCCTTGTTTGTTCTGAGATTAATCTAGCTTTTGTACCTATGCATACGTGGTAGGTAGATTCAGGTGCTACTACTCACATAAGTGTATCTATGCAGGGTTGCATTTGAAGCCGACTGCCAAGTGATGCTGAGACTTTCATCTACGTGGCTGACGGCAATAGGGCAAAAGTAGAAACAATAGGAACATTTAGATTATCTTTAGGAACTGGTTTTCATTTGGATTTGTTTGAGACTTTTGTTGTTCCGTCATTTAGACGGAATTTAATTTCTGTTTCTTCATTGGACAAATTTGGTTATTCTTGTTCATTTGGAAATAACAAACTAAGTCTTTT

mRNA sequence

ATGCAAATCTCAAATGTTGCAACAAAACGTAAGGCACTGAAGTTGGAAGTTTCTGAAGACTTTTTAGTGCACTTAGTTTTGAATTCTCTTCCAGCAGAGTATAGCCACTTCAGGGTGCGTTACAACACTCAGAAGGATAAATGGTCCCTGAATGAGCTAATCTCTCACTGTGTTCAAGAGGAAGAGAGGATGCAGCGAGAGAAGACAGAAAGTGTTCACATGGCTTCTACCTCAAAGAGTGTAAAGAGAAAGAGAGTGAATAATGCTGCGGAATCTTCTAAGCAGAAAAAGGAAAAGAAACAGGATTCAGGACCTGCTTGTTTCTTCTGTAAGAAGACTGGGCACATGAAAAAACAATGTGCCAAATATACTGCATGGCTATTGAAGAAGGGGTTGCATTTGAAGCCGACTGCCAAGTGA

Coding sequence (CDS)

ATGCAAATCTCAAATGTTGCAACAAAACGTAAGGCACTGAAGTTGGAAGTTTCTGAAGACTTTTTAGTGCACTTAGTTTTGAATTCTCTTCCAGCAGAGTATAGCCACTTCAGGGTGCGTTACAACACTCAGAAGGATAAATGGTCCCTGAATGAGCTAATCTCTCACTGTGTTCAAGAGGAAGAGAGGATGCAGCGAGAGAAGACAGAAAGTGTTCACATGGCTTCTACCTCAAAGAGTGTAAAGAGAAAGAGAGTGAATAATGCTGCGGAATCTTCTAAGCAGAAAAAGGAAAAGAAACAGGATTCAGGACCTGCTTGTTTCTTCTGTAAGAAGACTGGGCACATGAAAAAACAATGTGCCAAATATACTGCATGGCTATTGAAGAAGGGGTTGCATTTGAAGCCGACTGCCAAGTGA

Protein sequence

MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQEEERMQREKTESVHMASTSKSVKRKRVNNAAESSKQKKEKKQDSGPACFFCKKTGHMKKQCAKYTAWLLKKGLHLKPTAK

Homology

[Close]

BLAST of MC00g_new0425.1 vs. NCBI nr
Match: XP_040867871.1 (uncharacterized protein LOC121174006 [Glycine max])

HSP 1 Score: 166 bits (420), Expect = 1.27e-49
Identity = 86/139 (61.87%), Postives = 109/139 (78.42%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M+ISN+A+K K LKLE+ ED L+HLVL SLPA +  F+V YNTQKDKWSL ELISHCVQE
Sbjct: 24  MEISNLASKMKTLKLELGEDLLMHLVLISLPAHFRQFKVSYNTQKDKWSLYELISHCVQE 83

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAESSKQKKEKKQDSGPACFFCKKTGHMKKQC 120
           EER+ R+ +ES H++STS++ KRK+   AAE S Q+K+ K+D    C FCKK+GHMKK+C
Sbjct: 84  EERLWRDISESTHLSSTSQNKKRKKTKGAAEGSFQQKKPKKDEEFTCCFCKKSGHMKKEC 143

Query: 121 AKYTAWLLKKGLHLKPTAK 139
            KY  W +KKGL ++PTAK
Sbjct: 144 PKYATWRVKKGLLVEPTAK 162

[Close]

BLAST of MC00g_new0425.1 vs. NCBI nr
Match: KYP49135.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan])

HSP 1 Score: 165 bits (417), Expect = 1.26e-47
Identity = 85/132 (64.39%), Postives = 105/132 (79.55%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M++SN+ +K KALKLE+S D LVHLVL SLP  +  F+V YNTQKDKW+LNELISHCVQE
Sbjct: 118 MEMSNLVSKLKALKLELSNDLLVHLVLISLPTHFGQFKVSYNTQKDKWTLNELISHCVQE 177

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAE-SSKQKKEKKQDSGPACFFCKKTGHMKKQ 120
           EER QREKTES H+AS+S++ KRK   +  E +S+QKK KK ++ P CFFCKK GHMKK+
Sbjct: 178 EERQQREKTESAHLASSSQNRKRKNNKDVVERTSQQKKVKKDENTPTCFFCKKPGHMKKE 237

Query: 121 CAKYTAWLLKKG 131
           C KY +W +KKG
Sbjct: 238 CPKYASWRVKKG 249

[Close]

BLAST of MC00g_new0425.1 vs. NCBI nr
Match: XP_024641115.1 (uncharacterized protein LOC112422451 [Medicago truncatula])

HSP 1 Score: 164 bits (416), Expect = 1.93e-47
Identity = 86/139 (61.87%), Postives = 113/139 (81.29%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           +++SN+A+K KALKLE+ ED +VHLVL SLP+ +S F+V YNTQK+KW+LNELISHCVQE
Sbjct: 151 LEMSNLASKLKALKLELPEDLIVHLVLISLPSHFSQFKVSYNTQKEKWTLNELISHCVQE 210

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAESSKQKKEKKQDSGPACFFCKKTGHMKKQC 120
           EER++REKTES H+A+ S++ KRKR   A  SS+ KK K+Q +G  CFFCKK GHMK  C
Sbjct: 211 EERLKREKTESAHLATNSQN-KRKRT--AGNSSQNKKAKEQPAGVKCFFCKKYGHMKNDC 270

Query: 121 AKYTAWLLKKGLHLKPTAK 139
           +K+ AW +KKGL ++P+AK
Sbjct: 271 SKFAAWKVKKGLPVEPSAK 286

[Close]

BLAST of MC00g_new0425.1 vs. NCBI nr
Match: RDX60874.1 (hypothetical protein CR513_60950, partial [Mucuna pruriens])

HSP 1 Score: 160 bits (404), Expect = 3.21e-47
Identity = 81/130 (62.31%), Postives = 102/130 (78.46%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M++SN+  K K+LKLE++ED LVHLVL SLPA +  F+V YNTQKDKWSLNELISH VQE
Sbjct: 1   MEMSNLTAKLKSLKLEIAEDLLVHLVLISLPAHFGQFKVSYNTQKDKWSLNELISHYVQE 60

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAESSKQKKEKKQDSGPACFFCKKTGHMKKQC 120
           EER+QR+KTES +  STS++ KRK++   AE S Q+K+ K++    C+FCKK GHMKKQC
Sbjct: 61  EERLQRDKTESAYFVSTSQNKKRKKIKGVAEGSSQRKKLKKNEKFTCYFCKKLGHMKKQC 120

Query: 121 AKYTAWLLKK 130
            KY AW +KK
Sbjct: 121 PKYAAWRVKK 130

[Close]

BLAST of MC00g_new0425.1 vs. NCBI nr
Match: KYP40660.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94, partial [Cajanus cajan])

HSP 1 Score: 165 bits (417), Expect = 9.47e-47
Identity = 85/132 (64.39%), Postives = 105/132 (79.55%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M++SN+A+K KALKLE+S+D LVHLVL SLP  +  F+V YNTQKDKW+LNELISHCVQE
Sbjct: 151 MEMSNLASKLKALKLELSDDLLVHLVLISLPTHFGQFKVSYNTQKDKWTLNELISHCVQE 210

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAESSKQ-KKEKKQDSGPACFFCKKTGHMKKQ 120
           EER QREKTES H+AS+S++ KRK   +  E + Q KK KK ++ P CFFCKK GHMKK+
Sbjct: 211 EERQQREKTESAHLASSSQNRKRKNNKDVVERTSQLKKVKKDENTPTCFFCKKPGHMKKE 270

Query: 121 CAKYTAWLLKKG 131
           C KY +W +KKG
Sbjct: 271 CPKYVSWRVKKG 282

[Close]

BLAST of MC00g_new0425.1 vs. ExPASy TrEMBL
Match: A0A151S2S4 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_029167 PE=4 SV=1)

HSP 1 Score: 165 bits (417), Expect = 6.10e-48
Identity = 85/132 (64.39%), Postives = 105/132 (79.55%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M++SN+ +K KALKLE+S D LVHLVL SLP  +  F+V YNTQKDKW+LNELISHCVQE
Sbjct: 118 MEMSNLVSKLKALKLELSNDLLVHLVLISLPTHFGQFKVSYNTQKDKWTLNELISHCVQE 177

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAE-SSKQKKEKKQDSGPACFFCKKTGHMKKQ 120
           EER QREKTES H+AS+S++ KRK   +  E +S+QKK KK ++ P CFFCKK GHMKK+
Sbjct: 178 EERQQREKTESAHLASSSQNRKRKNNKDVVERTSQQKKVKKDENTPTCFFCKKPGHMKKE 237

Query: 121 CAKYTAWLLKKG 131
           C KY +W +KKG
Sbjct: 238 CPKYASWRVKKG 249

[Close]

BLAST of MC00g_new0425.1 vs. ExPASy TrEMBL
Match: A0A371E4C3 (CCHC-type domain-containing protein (Fragment) OS=Mucuna pruriens OX=157652 GN=CR513_60950 PE=4 SV=1)

HSP 1 Score: 160 bits (404), Expect = 1.56e-47
Identity = 81/130 (62.31%), Postives = 102/130 (78.46%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M++SN+  K K+LKLE++ED LVHLVL SLPA +  F+V YNTQKDKWSLNELISH VQE
Sbjct: 1   MEMSNLTAKLKSLKLEIAEDLLVHLVLISLPAHFGQFKVSYNTQKDKWSLNELISHYVQE 60

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAESSKQKKEKKQDSGPACFFCKKTGHMKKQC 120
           EER+QR+KTES +  STS++ KRK++   AE S Q+K+ K++    C+FCKK GHMKKQC
Sbjct: 61  EERLQRDKTESAYFVSTSQNKKRKKIKGVAEGSSQRKKLKKNEKFTCYFCKKLGHMKKQC 120

Query: 121 AKYTAWLLKK 130
            KY AW +KK
Sbjct: 121 PKYAAWRVKK 130

[Close]

BLAST of MC00g_new0425.1 vs. ExPASy TrEMBL
Match: A0A151RDF9 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Cajanus cajan OX=3821 GN=KK1_038014 PE=4 SV=1)

HSP 1 Score: 165 bits (417), Expect = 4.58e-47
Identity = 85/132 (64.39%), Postives = 105/132 (79.55%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M++SN+A+K KALKLE+S+D LVHLVL SLP  +  F+V YNTQKDKW+LNELISHCVQE
Sbjct: 151 MEMSNLASKLKALKLELSDDLLVHLVLISLPTHFGQFKVSYNTQKDKWTLNELISHCVQE 210

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAESSKQ-KKEKKQDSGPACFFCKKTGHMKKQ 120
           EER QREKTES H+AS+S++ KRK   +  E + Q KK KK ++ P CFFCKK GHMKK+
Sbjct: 211 EERQQREKTESAHLASSSQNRKRKNNKDVVERTSQLKKVKKDENTPTCFFCKKPGHMKKE 270

Query: 121 CAKYTAWLLKKG 131
           C KY +W +KKG
Sbjct: 271 CPKYVSWRVKKG 282

[Close]

BLAST of MC00g_new0425.1 vs. ExPASy TrEMBL
Match: A0A151S1H3 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_029698 PE=4 SV=1)

HSP 1 Score: 160 bits (405), Expect = 1.07e-46
Identity = 84/130 (64.62%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 3   ISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQEEE 62
           +SN+A+K KALKLE+S+D  VHLVL SL   +  F+V YNTQKDKW+LNELISHCVQEEE
Sbjct: 1   MSNLASKLKALKLELSDDLFVHLVLISLLTHFGQFKVSYNTQKDKWTLNELISHCVQEEE 60

Query: 63  RMQREKTESVHMASTSKSVKRKRVNNAAE-SSKQKKEKKQDSGPACFFCKKTGHMKKQCA 122
           R QREKTES H+AS+S+S KRK   +  E +S+QKK KK ++ P CFFCKK GHMKK+C 
Sbjct: 61  RQQREKTESAHLASSSQSRKRKNNKDVVERTSQQKKVKKDENTPTCFFCKKPGHMKKECP 120

Query: 123 KYTAWLLKKG 131
           KY +W +KKG
Sbjct: 121 KYASWRVKKG 130

[Close]

BLAST of MC00g_new0425.1 vs. ExPASy TrEMBL
Match: A0A392R0S9 (Putative WRKY transcription factor 72-like (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1)

HSP 1 Score: 157 bits (398), Expect = 1.43e-46
Identity = 82/131 (62.60%), Postives = 102/131 (77.86%), Query Frame = 0


Query: 1   MQISNVATKRKALKLEVSEDFLVHLVLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQE 60
           M++SN+A+K KALKLE+ ED +VHLVL SLPA +  F+V YNTQKDKW+LNELISHCVQE
Sbjct: 34  MEMSNLASKLKALKLELPEDLIVHLVLISLPAHFGQFKVSYNTQKDKWTLNELISHCVQE 93

Query: 61  EERMQREKTESVHMASTSKSVKRKRVNNAAESSKQKKEKKQDSGPACFFCKKTGHMKKQC 120
           EER++REKTES H+A++S++   KR   A + S+ KK K+Q     CFFCKK GHMKK C
Sbjct: 94  EERLKREKTESAHLATSSQN---KRKKTAVKFSQNKKAKEQPKESTCFFCKKVGHMKKDC 153

Query: 121 AKYTAWLLKKG 131
           +KY AW  KKG
Sbjct: 154 SKYAAWREKKG 161

[Close]

BLAST of MC00g_new0425.1 vs. TAIR 10
Match: AT5G53690.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 49.3 bits (116), Expect = 2.9e-06
Identity = 31/70 (44.29%), Postives = 43/70 (61.43%), Query Frame = 0


Query: 26 VLNSLPAEYSHFRVRYNTQKDKWSLNELISHCVQEEERMQREKTESVHMASTSKSV---K 85
          VL+SLP++Y   R  Y+  K +WS ++LISHCVQEEER+  EK E  H     K +   K
Sbjct: 5  VLDSLPSDYDLLRKTYSRMKLEWSTHDLISHCVQEEERLTNEKKE--HDLVAGKVICDKK 64

Query: 86 RKRVNNAAES 93
          RK+ +   E+
Sbjct: 65 RKQYDECLEA 72

The following BLAST results are available for this feature:

BLAST of MC00g_new0425.1 vs.


Match Name	E-value	Identity	Description

BLAST of MC00g_new0425.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_040867871.1	1.27e-49	61.87	uncharacterized protein LOC121174006 [Glycine max]	[more]
KYP49135.1	1.26e-47	64.39	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan]	[more]
XP_024641115.1	1.93e-47	61.87	uncharacterized protein LOC112422451 [Medicago truncatula]	[more]
RDX60874.1	3.21e-47	62.31	hypothetical protein CR513_60950, partial [Mucuna pruriens]	[more]
KYP40660.1	9.47e-47	64.39	Retrovirus-related Pol polyprotein from transposon TNT 1-94, partial [Cajanus ca...	[more]

BLAST of MC00g_new0425.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A151S2S4	6.10e-48	64.39	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=...	[more]
A0A371E4C3	1.56e-47	62.31	CCHC-type domain-containing protein (Fragment) OS=Mucuna pruriens OX=157652 GN=C...	[more]
A0A151RDF9	4.58e-47	64.39	Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Cajanu...	[more]
A0A151S1H3	1.07e-46	64.62	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=...	[more]
A0A392R0S9	1.43e-46	62.60	Putative WRKY transcription factor 72-like (Fragment) OS=Trifolium medium OX=970...	[more]

BLAST of MC00g_new0425.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53690.1	2.9e-06	44.29	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 50..70
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 4..66 e-value: 2.2E-9 score: 37.2
None	No IPR available	GENE3D	4.10.60.10		coord: 74..131 e-value: 4.8E-6 score: 28.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 64..106
None	No IPR available	PANTHER	PTHR35317:SF12	OS04G0629600 PROTEIN	coord: 1..124
None	No IPR available	PANTHER	PTHR35317	OS04G0629600 PROTEIN	coord: 1..124
IPR001878	Zinc finger, CCHC-type	SMART	SM00343	c2hcfinal6	coord: 106..122 e-value: 0.0073 score: 24.4
IPR001878	Zinc finger, CCHC-type	PFAM	PF00098	zf-CCHC	coord: 106..122 e-value: 2.5E-4 score: 21.0
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 107..120 score: 8.89451
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	57756	Retrovirus zinc finger-like domains	coord: 92..125

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC00g_new0425	MC00g_new0425	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC00g_new0425.1-cds	MC00g_new0425.1-cds-scaffold7:184248..184638	CDS
MC00g_new0425.1-cds	MC00g_new0425.1-cds-scaffold7:184746..184774	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC00g_new0425.1	MC00g_new0425.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding