MC00g0349.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0349.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	trans-resveratrol di-O-methyltransferase-like
Location	scaffold7: 157068 .. 158447 (-)
Sequence length	1038
RNA-Seq Expression	MC00g0349.1
Synteny	MC00g0349.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAAGTTGTAGGCGAGGAGGTTTTGGAAGCGGAGACTCATATATGGAACCATATCTTCAGCTTCATCAACTTCATGGCTTTGAAATGCGTCGTCGAGCTCGACATTCCTGATGCCATCGCCCGCCACGGCCACCGCCCCATGCCCTTCTCTCACCACGTTTCAGCCCTTAAACTTCACCCCCACAACTCCCAATACCTTCATCGCTCAAGGCGTATACTAGATCACTATGGCTTCTTTACCAAATACAAAAATGGCAAAGAAGAAGAAGCATACTCCCTCACCAACTCGTCTCGTCTTCTCTTCAATCATAATCATTCCTCTCTATCTCCCTTGTTGCTTTCCCTGCTCGACTCGTTCCTCTTGCAACCATGGCAGCTTTTATCCGCTTGGTTCTATAGCCATGATCGAATCCCGTTTAAGATGGCTCATGGAAAGCAGTTCTGGGAGCACACAGCCAGCAAGCCACAAGATGGGGACACTTTTAATGCAGCCATGGCCAATGATGTGAGGCTGGTGACAAGTGTTTTGCTCGGACAATATAAGAACATGTTTGTTGGAGTTAAGTCGCTGGTGGATGTGGGAGGTGGCATTGGAATTTTGGCAAGAGTCATTGCTGAGGCCTTTCTGCAAACACAATGTGTTGTGCTTGATCTTCTCCATGTGCTGACTAACTTGGAAGAAAGCCATAACCTCATATATCCTGAAGGGGAAATGTTTCAAAGCATTCCTTTGCCAATGTACTTCTATTGAAGGATAGAGAAAAAAAAAACTACAAATATATATTATTTAGTTTTGGGGACCAAATGTTTTGTTAAAAGGATCTACTCCTCTCATTGTCGATTGAGATGGAAGTTCATGAAAATTGATATGGTATCAGAACCTAATAAGGTTAAAGTGAACATCTAGTAGAAAAAGGGGATTCGGCCAAAATTGATGAACTTGAAAGAGACACCATCTTGAAGGGCATGTTAATGATCTACTCCTCTCACTGTCAACTGATTTTGAGCTGAAACCATGAAACTTGATACTTTTGTTGTTATAAAGTTGTTGAAACTATTTTTTTTTAGTATTATTAAAGAGTAATGTCATTTGATATAAATGCATGATTGGAGCAATGGACAATGTGTGGAAATACTGAAGAAATGCAAAGGTGCAATCACGGGTAATGGAAAGAAAGGGAAAGTGATTCTAATTGATATGGTAGGCAATAAGAAGGGGGGCAGGGATGGAGACGACATGTTTATTGAAACTCAACTCTTTTGGGACATGTTAATGATGGCGAACGTAGGTGGAAAAGAACAAGATAAGAAAGAGTGGGTTCACCTGTTGCATAAGGCTGAATTTGGTGGCCACAAGATCTTCCCAATTTTAGGT

mRNA sequence

ATGGGAGAAGTTGTAGGCGAGGAGGTTTTGGAAGCGGAGACTCATATATGGAACCATATCTTCAGCTTCATCAACTTCATGGCTTTGAAATGCGTCGTCGAGCTCGACATTCCTGATGCCATCGCCCGCCACGGCCACCGCCCCATGCCCTTCTCTCACCACGTTTCAGCCCTTAAACTTCACCCCCACAACTCCCAATACCTTCATCGCTCAAGGCGTATACTAGATCACTATGGCTTCTTTACCAAATACAAAAATGGCAAAGAAGAAGAAGCATACTCCCTCACCAACTCGTCTCGTCTTCTCTTCAATCATAATCATTCCTCTCTATCTCCCTTGTTGCTTTCCCTGCTCGACTCGTTCCTCTTGCAACCATGGCAGCTTTTATCCGCTTGGTTCTATAGCCATGATCGAATCCCGTTTAAGATGGCTCATGGAAAGCAGTTCTGGGAGCACACAGCCAGCAAGCCACAAGATGGGGACACTTTTAATGCAGCCATGGCCAATGATGTGAGGCTGGTGACAAGTGTTTTGCTCGGACAATATAAGAACATGTTTGTTGGAGTTAAGTCGCTGGTGGATGTGGGAGGTGGCATTGGAATTTTGGCAAGAGTCATTGCTGAGGCCTTTCTGCAAACACAATGTGTTGTGCTTGATCTTCTCCATGTGCTGACTAACTTGGAAGAAAGCCATAACCTCATATATCCTGAAGGGGAAATGTTTCAAAGCATTCCTTTGCCAATAGTAATGTCATTTGATATAATGCATGATTGGAGCAATGGACAATGTGTGGAAATACTGAAGAAATGCAAAGGTGCAATCACGGGTAATGGAAAGAAAGGGAAAGTGATTCTAATTGATATGGTAGGCAATAAGAAGGGGGGCAGGGATGGAGACGACATGTTTATTGAAACTCAACTCTTTTGGGACATGTTAATGATGGCGAACGTAGGTGGAAAAGAACAAGATAAGAAAGAGTGGGTTCACCTGTTGCATAAGGCTGAATTTGGTGGCCACAAGATCTTCCCAATTTTAGGT

Coding sequence (CDS)

ATGGGAGAAGTTGTAGGCGAGGAGGTTTTGGAAGCGGAGACTCATATATGGAACCATATCTTCAGCTTCATCAACTTCATGGCTTTGAAATGCGTCGTCGAGCTCGACATTCCTGATGCCATCGCCCGCCACGGCCACCGCCCCATGCCCTTCTCTCACCACGTTTCAGCCCTTAAACTTCACCCCCACAACTCCCAATACCTTCATCGCTCAAGGCGTATACTAGATCACTATGGCTTCTTTACCAAATACAAAAATGGCAAAGAAGAAGAAGCATACTCCCTCACCAACTCGTCTCGTCTTCTCTTCAATCATAATCATTCCTCTCTATCTCCCTTGTTGCTTTCCCTGCTCGACTCGTTCCTCTTGCAACCATGGCAGCTTTTATCCGCTTGGTTCTATAGCCATGATCGAATCCCGTTTAAGATGGCTCATGGAAAGCAGTTCTGGGAGCACACAGCCAGCAAGCCACAAGATGGGGACACTTTTAATGCAGCCATGGCCAATGATGTGAGGCTGGTGACAAGTGTTTTGCTCGGACAATATAAGAACATGTTTGTTGGAGTTAAGTCGCTGGTGGATGTGGGAGGTGGCATTGGAATTTTGGCAAGAGTCATTGCTGAGGCCTTTCTGCAAACACAATGTGTTGTGCTTGATCTTCTCCATGTGCTGACTAACTTGGAAGAAAGCCATAACCTCATATATCCTGAAGGGGAAATGTTTCAAAGCATTCCTTTGCCAATAGTAATGTCATTTGATATAATGCATGATTGGAGCAATGGACAATGTGTGGAAATACTGAAGAAATGCAAAGGTGCAATCACGGGTAATGGAAAGAAAGGGAAAGTGATTCTAATTGATATGGTAGGCAATAAGAAGGGGGGCAGGGATGGAGACGACATGTTTATTGAAACTCAACTCTTTTGGGACATGTTAATGATGGCGAACGTAGGTGGAAAAGAACAAGATAAGAAAGAGTGGGTTCACCTGTTGCATAAGGCTGAATTTGGTGGCCACAAGATCTTCCCAATTTTAGGT

Protein sequence

MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDSFLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMFQSIPLPIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGDDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG

Homology

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BLAST of MC00g0349.1 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 310.1 bits (793), Expect = 3.2e-83
Identity = 165/346 (47.69%), Postives = 223/346 (64.45%), Query Frame = 0


Query: 4   VVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPH 63
           V+  E+L A+ H+WNHIF+FI  M+LKC ++L IPD I  HG +PM     V+ L +HP 
Sbjct: 7   VISAELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPELVAKLPVHPK 66

Query: 64  NSQYLHRSRRILDHYGFFT--KYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDSF 123
            SQ ++R  RIL H GF    + + GKEEE Y LT++SRLL   +  S+ PL+L++LD  
Sbjct: 67  RSQCVYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPI 126

Query: 124 LLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQ 183
           L +PW  LSAWF + D  PF  A+ + FW++   +PQ  ++FN AMA+D RL+TSVLL +
Sbjct: 127 LTKPWHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKE 186

Query: 184 YKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMF 243
            + +F G+ SLVDVGGG G +A+ IA AF    C VLDL HV+  L+ S NL Y  G+MF
Sbjct: 187 GQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSKNLNYFAGDMF 246

Query: 244 QSI-PLPIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGDD 303
           ++I P   ++   I+HDWSN +CV+ILK+C+ AI      GKVI+IDM+  K     GD 
Sbjct: 247 EAIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMK---NQGDY 306

Query: 304 MFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 347
              ETQLF+DM MM    G+E+D+ EW  L   A F  +KI PILG
Sbjct: 307 KSTETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILG 348

[Close]

BLAST of MC00g0349.1 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.8e-79
Identity = 168/355 (47.32%), Postives = 228/355 (64.23%), Query Frame = 0


Query: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60
           + E    E+L A+ HIWN IF+FIN M+LKC ++L IPD I  HG +PM  S    AL +
Sbjct: 18  LNEENSTEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPI 77

Query: 61  HPHNSQYLHRSRRILDHYGFFTKYK------NGKEEEAYSLTNSSRLLFNHNHSSLSPLL 120
           +   S  ++R  RIL H GFF   K       G EEE Y +T++S+LL   N  S++P L
Sbjct: 78  NRKKSPCVYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFL 137

Query: 121 LSLLDSFLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLV 180
           L++LD  + +PW  LS WF + D  PF  A+G  FW++ + +P+    FN AMA+D RLV
Sbjct: 138 LAMLDPVMTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLV 197

Query: 181 TSVLLGQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLI 240
           TSV++ + K +F GV+SLVDVGGG G +A  IA AF   QC V DL HV+ +L+  +NL 
Sbjct: 198 TSVVIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLN 257

Query: 241 YPEGEMFQSIP-LPIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKK-GKVILIDM-VGN 300
           +  G+MF  +P   +V+   I+HDW++ + V+ILKKCK AI+ + KK GKVI+IDM V N
Sbjct: 258 FVGGDMFVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVEN 317

Query: 301 KKGGRDGDDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 347
           +K   D DD   ETQLF+DMLMMA V G+E+++KEW  L   A F  +KI PILG
Sbjct: 318 EK---DEDDESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILG 368

[Close]

BLAST of MC00g0349.1 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 150/345 (43.48%), Postives = 208/345 (60.29%), Query Frame = 0


Query: 6   GEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPHNS 65
           GEE+L A+ HI+NHIF+FI+ MALKC VEL+IP+ +  H  + +  S  V AL++    S
Sbjct: 21  GEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSELVQALQIPQAKS 80

Query: 66  QYLHRSRRILDHYGFF--TKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDSFLL 125
             L+R  RIL H GFF  TK ++  +EE Y  T SS+LL  ++  S+SP LL L++  ++
Sbjct: 81  ACLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLKNHPMSMSPCLLGLVNPTMV 140

Query: 126 QPWQLLSAWF-YSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQY 185
            P    S WF  S D  PF+  HG   W++    P   + FN AM  + RL  SV+L + 
Sbjct: 141 APMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEIFNEAMGCETRLAMSVVLKEC 200

Query: 186 KNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMFQ 245
           K    G+ SLVDVGGG G + R IAEAF   +C VLDL  V+ NL+ S+NL +  G+MFQ
Sbjct: 201 KGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGSNNLEFVSGDMFQ 260

Query: 246 SI-PLPIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGDDM 305
            I P  +V    I+HDW++ +C++ILK+CK AI    + GK+I+IDMV N      G   
Sbjct: 261 FIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVNDH--NKGSYE 320

Query: 306 FIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 347
             ETQLF+D+ +MA + G E+ + EW  L   A F  + I P+LG
Sbjct: 321 STETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLG 363

[Close]

BLAST of MC00g0349.1 vs. ExPASy Swiss-Prot
Match: B0EXJ8 (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.0e-65
Identity = 142/351 (40.46%), Postives = 211/351 (60.11%), Query Frame = 0


Query: 3   EVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSAL--KL 62
           +V  EE   A+  IW+   SFI   +LKC V+L IPD I  HG +P+  S   +AL   +
Sbjct: 2   DVQSEEFRGAQAQIWSQSCSFITSASLKCAVKLGIPDTIDNHG-KPITLSELTNALVPPV 61

Query: 63  HPHNSQYLHRSRRILDHYGFFTKYK-NGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLD 122
           HP  + +++R  R+L   GF ++ + +G+ E  YSLT SSR+L      +L  ++L++ D
Sbjct: 62  HPSKAPFIYRLMRVLAKNGFCSEEQLDGETEPLYSLTPSSRILLKKEPLNLRGIVLTMAD 121

Query: 123 SFLLQPWQLLSAWFYSHD--RIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSV 182
              L+ W+ LS W+ + D     F+ AHGK FW +++   +  + FN AMA+D +L++ +
Sbjct: 122 PVQLKAWESLSDWYQNEDDSSTAFETAHGKNFWGYSSEHMEHAEFFNEAMASDSQLISKL 181

Query: 183 LLGQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPE 242
           L+G+YK +F G+ SLVD+GGG G +A+ IA+ F Q +C V DL HV+ NLE   N+ +  
Sbjct: 182 LIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKENVEFVA 241

Query: 243 GEMFQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMV--GNKKG 302
           G+MF+ IP    +    I+HDW++  CV+ILK CK AI   G  GKVI+IDMV   +KK 
Sbjct: 242 GDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKG--GKVIIIDMVMYSDKK- 301

Query: 303 GRDGDDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPIL 346
               DD  ++TQ   DM M+ N   KE+ +KEW  L  +A F  +KI+P L
Sbjct: 302 ----DDHLVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKL 344

[Close]

BLAST of MC00g0349.1 vs. ExPASy Swiss-Prot
Match: Q93WU3 (Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.2e-63
Identity = 138/343 (40.23%), Postives = 202/343 (58.89%), Query Frame = 0


Query: 7   EEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPHNSQ 66
           E++L+A+ H+WNH+++F N M+LKC ++L IPD + +H H PM  S  + A+ ++   SQ
Sbjct: 13  EQLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKHDH-PMTLSQLLKAIPINKEKSQ 72

Query: 67  YLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDSFLLQPW 126
              R  R L +  FF +  +  +E  Y LT +SRLL      +++PL+  +LD     PW
Sbjct: 73  SFQRLMRALVNSNFFIEENSNNQEVCYWLTPASRLLLKGAPLTVAPLVQVVLDPTFTNPW 132

Query: 127 QLLSAWF-YSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQYKNM 186
             +S WF + +    F+ A+G  FWE  A+KP  G  F+ AM+ D RLV  VL   YK++
Sbjct: 133 HYMSEWFKHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTKDYKHV 192

Query: 187 FVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMFQSIP 246
             G+++LVDVGGG G +A+ I EA    +C VLDL HV+  LE +  L Y  G+MFQSIP
Sbjct: 193 IDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTDKLSYIGGDMFQSIP 252

Query: 247 L--PIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGDDMFI 306
               I++ F I+HDW + + ++ILK+CK A+   G  GKVI+ID+V    G     D  +
Sbjct: 253 SADAILLKF-IIHDWDDEEGLKILKRCKDAV---GIGGKVIIIDVV---VGVNHDVDEVL 312

Query: 307 ETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 347
           E QL +DM MM+    KE+   EW  L+  A F  +K+ P  G
Sbjct: 313 EDQLHFDMAMMSYFNAKERTMNEWEKLISAAGFTSYKLTPAFG 347

[Close]

BLAST of MC00g0349.1 vs. NCBI nr
Match: XP_022156551.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 518 bits (1335), Expect = 2.60e-182
Identity = 270/353 (76.49%), Postives = 291/353 (82.44%), Query Frame = 0


Query: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60
           MGEV G EVLEAETHIWNHIFSFIN MALKC V+L IPD IA HG  PM  SH VSALKL
Sbjct: 1   MGEVAGNEVLEAETHIWNHIFSFINSMALKCAVQLGIPDTIAHHGRGPMSLSHLVSALKL 60

Query: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKE-EEAYSLTNSSRLLFNHNHSSLSPLLLSLLD 120
           HP NSQYLHR  RIL H GFFT +KN KE EEAYSLT+SSRLL   N SSLSPLLLS+LD
Sbjct: 61  HPENSQYLHRLMRILAHSGFFTAHKNDKEQEEAYSLTSSSRLLLKCNPSSLSPLLLSMLD 120

Query: 121 SFLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLL 180
            FLLQPWQLLSAWF+SHDR  FKMAHGKQFWEH ASKP+DGD FNAAMA+D RLVTSVLL
Sbjct: 121 PFLLQPWQLLSAWFHSHDRTAFKMAHGKQFWEHMASKPRDGDVFNAAMASDARLVTSVLL 180

Query: 181 GQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGE 240
           GQYKN+F GVKSLVDVGGG G +ARVIA+ F Q QCVVLDL HV+ +LE SHNL Y EG+
Sbjct: 181 GQYKNIFDGVKSLVDVGGGTGTVARVIADVFPQIQCVVLDLPHVVADLEGSHNLTYLEGD 240

Query: 241 MFQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMV-GNKK---- 300
           MFQSIP   V+    I+HDWS+G+C EILKKCK AIT NGKKG+VILIDMV GNKK    
Sbjct: 241 MFQSIPSADVLLLKWILHDWSDGECAEILKKCKEAITSNGKKGRVILIDMVVGNKKVDGD 300

Query: 301 GGRDGDDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
           G RDGDDMFIETQLFWDMLMM NVGGKE+D+KEWV L  KA FGG+KIFPILG
Sbjct: 301 GDRDGDDMFIETQLFWDMLMMVNVGGKERDEKEWVQLFDKAGFGGYKIFPILG 353

[Close]

BLAST of MC00g0349.1 vs. NCBI nr
Match: XP_022156562.1 (trans-resveratrol di-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 499 bits (1284), Expect = 2.65e-176
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60
           MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL
Sbjct: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60

Query: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS 120
           HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS
Sbjct: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS 120

Query: 121 FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 180
           FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG
Sbjct: 121 FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 180

Query: 181 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 240
           QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM
Sbjct: 181 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 240

Query: 241 FQSIPLPI 248
           FQSIPLP+
Sbjct: 241 FQSIPLPM 248

[Close]

BLAST of MC00g0349.1 vs. NCBI nr
Match: XP_022156559.1 (trans-resveratrol di-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 491 bits (1265), Expect = 8.71e-172
Identity = 251/348 (72.13%), Postives = 281/348 (80.75%), Query Frame = 0


Query: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60
           M E+ GEEVLEAE  IWNHIFSFIN MALKCVV+L IPDAIARH   PMP SH +SAL+L
Sbjct: 1   MEELAGEEVLEAEARIWNHIFSFINSMALKCVVQLGIPDAIARHDCSPMPLSHLISALQL 60

Query: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS 120
           HP NSQY+HR   IL H GFF  +KNG EEEAYSLTNSSRLL  HN SSLSP LLS+LD 
Sbjct: 61  HPQNSQYIHRLMCILAHSGFFITHKNGSEEEAYSLTNSSRLLLKHNPSSLSPFLLSMLDP 120

Query: 121 FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 180
            LLQPWQLLSAWF+S +R PF+MAHG+QFWEH  +K QDGD FNAAMA+D RLV+++LLG
Sbjct: 121 LLLQPWQLLSAWFHSLNRTPFEMAHGRQFWEHMGNKQQDGDVFNAAMASDARLVSNILLG 180

Query: 181 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 240
           QYKNMF GVKSLVDVGGG G +A+ IA+AF QTQCVVLDL HV+ +LE SHNL Y  G+M
Sbjct: 181 QYKNMFDGVKSLVDVGGGTGTMAKAIADAFPQTQCVVLDLFHVVADLEGSHNLTYLPGDM 240

Query: 241 FQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMV-GNKKGGRDG 300
           FQSIP   V+    I+HDWS+G+CVEILKKCK AIT NGK GKVILIDMV GNKKG    
Sbjct: 241 FQSIPPADVLLLKWILHDWSDGECVEILKKCKEAITSNGKTGKVILIDMVLGNKKG---- 300

Query: 301 DDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
           DD FIETQLFWDMLMMANV GKE+D+KEW  L  KA F G+KIFP+LG
Sbjct: 301 DDTFIETQLFWDMLMMANVSGKERDEKEWAQLFDKARFAGYKIFPVLG 344

[Close]

BLAST of MC00g0349.1 vs. NCBI nr
Match: XP_023548392.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387 bits (995), Expect = 1.49e-130
Identity = 205/350 (58.57%), Postives = 255/350 (72.86%), Query Frame = 0


Query: 6   GEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPHNS 65
           GEE+L A+ HIWNHIF+FIN MALKC V+L IPDAI+ HG +PM     VS+L++ P  S
Sbjct: 16  GEELLNAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHG-QPMTLPQLVSSLQVDPQKS 75

Query: 66  QYLHRSRRILDHYGFFTKYKNGKE----EEAYSLTNSSRLLFNHNHSSLSPLLLSLLDSF 125
           Q++HR  RIL + GFF  ++  KE    E AYSLTNSS LL  HN  +LSP LLS+LD  
Sbjct: 76  QHIHRLMRILAYAGFFLIHEPSKEGDDQEVAYSLTNSSLLLLEHNPLTLSPFLLSMLDPV 135

Query: 126 LLQPWQLLSAWFYS-HDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 185
           L+ PWQ +S W  S  DR PF+ AHGKQFWEH   KP+DG  FNAAMA+D RLVTSVLLG
Sbjct: 136 LVDPWQFISTWLRSDRDRTPFETAHGKQFWEHMGGKPKDGMVFNAAMASDARLVTSVLLG 195

Query: 186 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 245
           +YK++F GV+ LVDVGGG G + + IAEAF Q QCVV DL  V+ +L+ SHNL++ EG+M
Sbjct: 196 KYKSVFEGVELLVDVGGGTGTVGQAIAEAFPQMQCVVFDLPQVVADLKGSHNLMFVEGDM 255

Query: 246 FQSIP-LPIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGD 305
           FQ IP    ++   I+HDWS+ +CV+ILK+CK AIT NGKKGKV++ID+V      RD D
Sbjct: 256 FQQIPPAGAILLKWILHDWSDEECVKILKRCKEAITSNGKKGKVMIIDLV-----VRDED 315

Query: 306 ---DMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
              DM  ETQLFWD+LMM  VGG+E+++KEW  L +KA FGG+ IFPILG
Sbjct: 316 IKNDMLTETQLFWDVLMMVTVGGRERNEKEWAELFYKAGFGGYNIFPILG 359

[Close]

BLAST of MC00g0349.1 vs. NCBI nr
Match: XP_022135671.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 384 bits (985), Expect = 4.00e-129
Identity = 201/348 (57.76%), Postives = 250/348 (71.84%), Query Frame = 0


Query: 4   VVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPH 63
           V   E+LEA+ HIWNHIF+FIN MALKC V+L IPDAI RHG  PMP S  VSAL+L P 
Sbjct: 11  VPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQLVSALQLSPQ 70

Query: 64  NSQYLHRSRRILDHYGFFTKYK----NGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLD 123
            +QY++R  R+L H  FF  +K    NG EEEAY LTNSSRLL N N S+++P LLS+L+
Sbjct: 71  KTQYIYRLMRLLTHSSFFVTHKMMTKNG-EEEAYVLTNSSRLLLNDNPSAVTPFLLSMLE 130

Query: 124 SFLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLL 183
             L++PWQLL+AW  S DR PF   HG  FWE  + KP+DGD FN AMA+D RLV S+LL
Sbjct: 131 PALVEPWQLLAAWLQSEDRTPFHTTHGMPFWEFFSEKPKDGDVFNVAMASDARLVMSILL 190

Query: 184 GQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGE 243
            +YK +F GV SLVDVGGG G +A+ IA+AF Q +C+V DL  V+ NL+   NL Y EG 
Sbjct: 191 DKYKGVFEGVGSLVDVGGGTGTVAKAIAKAFPQIECIVFDLHQVVGNLKGEDNLKYVEGN 250

Query: 244 MFQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDG 303
           MF+++P    +    I+HDW++ +CVEILKKCK AIT NGKKGK+I+IDMV    G + G
Sbjct: 251 MFEAVPSADALLLKWILHDWNDEECVEILKKCKEAITNNGKKGKMIVIDMV---VGNKQG 310

Query: 304 DDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
           DD  IETQLF+DMLMM   GGKE+D+KEW  L+ +A   G+KIFPI+G
Sbjct: 311 DDS-IETQLFFDMLMMNLAGGKERDEKEWAQLIKEAGLSGYKIFPIMG 353

[Close]

BLAST of MC00g0349.1 vs. ExPASy TrEMBL
Match: A0A6J1DVA5 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111023429 PE=3 SV=1)

HSP 1 Score: 518 bits (1335), Expect = 1.26e-182
Identity = 270/353 (76.49%), Postives = 291/353 (82.44%), Query Frame = 0


Query: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60
           MGEV G EVLEAETHIWNHIFSFIN MALKC V+L IPD IA HG  PM  SH VSALKL
Sbjct: 1   MGEVAGNEVLEAETHIWNHIFSFINSMALKCAVQLGIPDTIAHHGRGPMSLSHLVSALKL 60

Query: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKE-EEAYSLTNSSRLLFNHNHSSLSPLLLSLLD 120
           HP NSQYLHR  RIL H GFFT +KN KE EEAYSLT+SSRLL   N SSLSPLLLS+LD
Sbjct: 61  HPENSQYLHRLMRILAHSGFFTAHKNDKEQEEAYSLTSSSRLLLKCNPSSLSPLLLSMLD 120

Query: 121 SFLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLL 180
            FLLQPWQLLSAWF+SHDR  FKMAHGKQFWEH ASKP+DGD FNAAMA+D RLVTSVLL
Sbjct: 121 PFLLQPWQLLSAWFHSHDRTAFKMAHGKQFWEHMASKPRDGDVFNAAMASDARLVTSVLL 180

Query: 181 GQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGE 240
           GQYKN+F GVKSLVDVGGG G +ARVIA+ F Q QCVVLDL HV+ +LE SHNL Y EG+
Sbjct: 181 GQYKNIFDGVKSLVDVGGGTGTVARVIADVFPQIQCVVLDLPHVVADLEGSHNLTYLEGD 240

Query: 241 MFQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMV-GNKK---- 300
           MFQSIP   V+    I+HDWS+G+C EILKKCK AIT NGKKG+VILIDMV GNKK    
Sbjct: 241 MFQSIPSADVLLLKWILHDWSDGECAEILKKCKEAITSNGKKGRVILIDMVVGNKKVDGD 300

Query: 301 GGRDGDDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
           G RDGDDMFIETQLFWDMLMM NVGGKE+D+KEWV L  KA FGG+KIFPILG
Sbjct: 301 GDRDGDDMFIETQLFWDMLMMVNVGGKERDEKEWVQLFDKAGFGGYKIFPILG 353

[Close]

BLAST of MC00g0349.1 vs. ExPASy TrEMBL
Match: A0A6J1DSB1 (trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111023437 PE=3 SV=1)

HSP 1 Score: 499 bits (1284), Expect = 1.28e-176
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60
           MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL
Sbjct: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60

Query: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS 120
           HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS
Sbjct: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS 120

Query: 121 FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 180
           FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG
Sbjct: 121 FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 180

Query: 181 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 240
           QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM
Sbjct: 181 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 240

Query: 241 FQSIPLPI 248
           FQSIPLP+
Sbjct: 241 FQSIPLPM 248

[Close]

BLAST of MC00g0349.1 vs. ExPASy TrEMBL
Match: A0A6J1DTU5 (trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111023435 PE=3 SV=1)

HSP 1 Score: 491 bits (1265), Expect = 4.22e-172
Identity = 251/348 (72.13%), Postives = 281/348 (80.75%), Query Frame = 0


Query: 1   MGEVVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKL 60
           M E+ GEEVLEAE  IWNHIFSFIN MALKCVV+L IPDAIARH   PMP SH +SAL+L
Sbjct: 1   MEELAGEEVLEAEARIWNHIFSFINSMALKCVVQLGIPDAIARHDCSPMPLSHLISALQL 60

Query: 61  HPHNSQYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDS 120
           HP NSQY+HR   IL H GFF  +KNG EEEAYSLTNSSRLL  HN SSLSP LLS+LD 
Sbjct: 61  HPQNSQYIHRLMCILAHSGFFITHKNGSEEEAYSLTNSSRLLLKHNPSSLSPFLLSMLDP 120

Query: 121 FLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 180
            LLQPWQLLSAWF+S +R PF+MAHG+QFWEH  +K QDGD FNAAMA+D RLV+++LLG
Sbjct: 121 LLLQPWQLLSAWFHSLNRTPFEMAHGRQFWEHMGNKQQDGDVFNAAMASDARLVSNILLG 180

Query: 181 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 240
           QYKNMF GVKSLVDVGGG G +A+ IA+AF QTQCVVLDL HV+ +LE SHNL Y  G+M
Sbjct: 181 QYKNMFDGVKSLVDVGGGTGTMAKAIADAFPQTQCVVLDLFHVVADLEGSHNLTYLPGDM 240

Query: 241 FQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMV-GNKKGGRDG 300
           FQSIP   V+    I+HDWS+G+CVEILKKCK AIT NGK GKVILIDMV GNKKG    
Sbjct: 241 FQSIPPADVLLLKWILHDWSDGECVEILKKCKEAITSNGKTGKVILIDMVLGNKKG---- 300

Query: 301 DDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
           DD FIETQLFWDMLMMANV GKE+D+KEW  L  KA F G+KIFP+LG
Sbjct: 301 DDTFIETQLFWDMLMMANVSGKERDEKEWAQLFDKARFAGYKIFPVLG 344

[Close]

BLAST of MC00g0349.1 vs. ExPASy TrEMBL
Match: A0A6J1C1F3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE=3 SV=1)

HSP 1 Score: 384 bits (985), Expect = 1.93e-129
Identity = 201/348 (57.76%), Postives = 250/348 (71.84%), Query Frame = 0


Query: 4   VVGEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPH 63
           V   E+LEA+ HIWNHIF+FIN MALKC V+L IPDAI RHG  PMP S  VSAL+L P 
Sbjct: 11  VPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQLVSALQLSPQ 70

Query: 64  NSQYLHRSRRILDHYGFFTKYK----NGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLD 123
            +QY++R  R+L H  FF  +K    NG EEEAY LTNSSRLL N N S+++P LLS+L+
Sbjct: 71  KTQYIYRLMRLLTHSSFFVTHKMMTKNG-EEEAYVLTNSSRLLLNDNPSAVTPFLLSMLE 130

Query: 124 SFLLQPWQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLL 183
             L++PWQLL+AW  S DR PF   HG  FWE  + KP+DGD FN AMA+D RLV S+LL
Sbjct: 131 PALVEPWQLLAAWLQSEDRTPFHTTHGMPFWEFFSEKPKDGDVFNVAMASDARLVMSILL 190

Query: 184 GQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGE 243
            +YK +F GV SLVDVGGG G +A+ IA+AF Q +C+V DL  V+ NL+   NL Y EG 
Sbjct: 191 DKYKGVFEGVGSLVDVGGGTGTVAKAIAKAFPQIECIVFDLHQVVGNLKGEDNLKYVEGN 250

Query: 244 MFQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDG 303
           MF+++P    +    I+HDW++ +CVEILKKCK AIT NGKKGK+I+IDMV    G + G
Sbjct: 251 MFEAVPSADALLLKWILHDWNDEECVEILKKCKEAITNNGKKGKMIVIDMV---VGNKQG 310

Query: 304 DDMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
           DD  IETQLF+DMLMM   GGKE+D+KEW  L+ +A   G+KIFPI+G
Sbjct: 311 DDS-IETQLFFDMLMMNLAGGKERDEKEWAQLIKEAGLSGYKIFPIMG 353

[Close]

BLAST of MC00g0349.1 vs. ExPASy TrEMBL
Match: A0A6J1JX64 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111488285 PE=3 SV=1)

HSP 1 Score: 382 bits (980), Expect = 4.26e-128
Identity = 200/347 (57.64%), Postives = 254/347 (73.20%), Query Frame = 0


Query: 6   GEEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPHNS 65
           GEE+L+A+ HIWNHIF+FIN MALKC V+L IPDAI+ HG +PM     VS+L++ P  S
Sbjct: 50  GEELLKAQAHIWNHIFNFINSMALKCAVQLRIPDAISHHG-QPMTLPQLVSSLQVDPQKS 109

Query: 66  QYLHRSRRILDHYGFFTKYKNGKE----EEAYSLTNSSRLLFNHNHSSLSPLLLSLLDSF 125
           Q++HR  RIL + GFF  ++  KE    E AYSLTNSS LL  HN  +LSP LLS+LD  
Sbjct: 110 QHIHRLMRILAYAGFFLIHEPSKEGDDQEVAYSLTNSSLLLLEHNPFTLSPFLLSMLDPV 169

Query: 126 LLQPWQLLSAWFYSH-DRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLG 185
           L+ PW+ +S W  +  DR PF+ AHGKQFWEH   KP+DG  FNAAMA+D RLVTSVLLG
Sbjct: 170 LVDPWKFISTWLRNDCDRTPFETAHGKQFWEHMGGKPKDGMVFNAAMASDSRLVTSVLLG 229

Query: 186 QYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEM 245
           ++K++F GV+ LVDVGGG G +A+ IAEAF Q QCVV DL  V+ +L+ SHNL + EG+M
Sbjct: 230 KHKSVFEGVELLVDVGGGTGTVAQAIAEAFPQMQCVVFDLPQVVGDLKGSHNLTFAEGDM 289

Query: 246 FQSIPLPIVMSFD-IMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGD 305
           FQ IP    +    I+HDWS+ +CVEILK+CK AIT NGKKGKV++ID+V   +  ++  
Sbjct: 290 FQQIPPADALLLKWILHDWSDEECVEILKRCKEAITSNGKKGKVMIIDLVVRDEDNKN-- 349

Query: 306 DMFIETQLFWDMLMMANVGGKEQDKKEWVHLLHKAEFGGHKIFPILG 346
           DM  ET+LFWD LMM  VGG+E+++KEW  L +KA FGG+ IFPILG
Sbjct: 350 DMLTETRLFWDELMMMTVGGRERNEKEWAELFYKAGFGGYTIFPILG 393

[Close]

BLAST of MC00g0349.1 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 152.9 bits (385), Expect = 4.7e-37
Identity = 114/349 (32.66%), Postives = 169/349 (48.42%), Query Frame = 0


Query: 7   EEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARH-GHRPMPFSHHVSALKLHPHNS 66
           +E  +A   IW ++F F +  A KC ++L IP+AI  H   +P+  +   SA+   P   
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASP--- 83

Query: 67  QYLHRSRRILDHYGFFTKY--KNGKEEEAYSLTNSSRLLF-NHNHSSLSPLLLSLLDSFL 126
            +L R  R L H G F +   K+G      +   S RL+    +  SL+P +L      +
Sbjct: 84  SHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEM 143

Query: 127 LQPWQLLSAWFYS----HDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVL 186
           L PW  LS+   S        PF   HGK  W      P   D  N AMA D R V   +
Sbjct: 144 LAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRV 203

Query: 187 LGQYKNMFVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEG 246
            G    +F GV ++VDVGGG G    ++ + F   +    DL HV+   E    +   EG
Sbjct: 204 AGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEG 263

Query: 247 EMFQSIP-LPIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMV--GNKKG- 306
           +MF SIP    +    ++HDW +  C++ILK CK A+  N   GKV++++ V   NKK  
Sbjct: 264 DMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPN--IGKVLIVESVIGENKKTM 323

Query: 307 -GRDGDDMFIETQLFWDMLMMANVG-GKEQDKKEWVHLLHKAEFGGHKI 342
              + D+     +L  DM+MMA+   GKE+  KEW  +L +A F  +++
Sbjct: 324 IVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEV 367

[Close]

BLAST of MC00g0349.1 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 5.0e-31
Identity = 102/342 (29.82%), Postives = 154/342 (45.03%), Query Frame = 0


Query: 7   EEVLEAETHIWNHIFSFINFMALKCVVELDIPDAIARH-GHRPMPFSHHVSALKLHPHNS 66
           +E  +A   IW ++F F +  A KC ++L IP+AI  H   +P+  S   SA+   P   
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASP--- 69

Query: 67  QYLHRSRRILDHYGFFTKYKNGKEEEAYSLTNSSRLLFNHNHSSLSPLLLSLLDSFLLQP 126
            +L R  R L H G F +    K+  A   TN             +PL            
Sbjct: 70  SHLRRIMRFLVHQGLFKEVPT-KDGLATGYTN-------------TPL------------ 129

Query: 127 WQLLSAWFYSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQYKNM 186
                       R+     HGK  W             N AMA D R V   + G  + +
Sbjct: 130 ----------SRRMMITKLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQGL 189

Query: 187 FVGVKSLVDVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMFQSIP 246
           F GV ++VDVGGG G    ++ + F   +    DL HV+   +    +   EG+MF SIP
Sbjct: 190 FDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIP 249

Query: 247 L-PIVMSFDIMHDWSNGQCVEILKKCKGAITGNGKKGKVILID-MVGNKKG---GRDGDD 306
               V+   ++HDW +  C++ILK CK A+  N   GKV++++ ++G KK      + DD
Sbjct: 250 ASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPN--IGKVLIVECVIGEKKNTMIAEERDD 309

Query: 307 MFIETQLFWDMLMMANVG-GKEQDKKEWVHLLHKAEFGGHKI 342
                +L  DM+MM +   GKE+  KEW  +L +A F  +++
Sbjct: 310 KLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEV 310

[Close]

BLAST of MC00g0349.1 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 113.2 bits (282), Expect = 4.1e-25
Identity = 97/323 (30.03%), Postives = 150/323 (46.44%), Query Frame = 0


Query: 27  MALKCVVELDIPDAIARHGHRPMPFSHHVSALKLHPHNSQYLHRSRRILDHYGFFT---- 86
           MALK  +ELD+ + +A++G    P          +P     L R  R+L  Y   T    
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 87  KYKNGKEEEAYSLTNSSRLLF-NHNHSSLSPLLLSLLDSFLLQPWQLLSAWFYSHDRIPF 146
           K      E  Y L    + L  N +  S++ L L   D  L++ W  L         IPF
Sbjct: 94  KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILD-GGIPF 153

Query: 147 KMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQYKNMFVGVKSLVDVGGGIGI 206
             A+G   +E+  + P+    FN  M+N   +    +L  YK  F G+ SLVDVGGGIG 
Sbjct: 154 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIGA 213

Query: 207 LARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMFQSIPL--PIVMSFDIMHDWS 266
             ++I   +   + +  DL HV+ +      + +  G+MF S+P    I M + I HDWS
Sbjct: 214 TLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKW-ICHDWS 273

Query: 267 NGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGDDMFIETQLFWDMLMMA-NVG 326
           +  CV+ LK C  ++    + GKVIL + +  +        +  +  +  D +M+A N G
Sbjct: 274 DEHCVKFLKNCYESLP---EDGKVILAECILPE---TPDSSLSTKQVVHVDCIMLAHNPG 333

Query: 327 GKEQDKKEWVHLLHKAEFGGHKI 342
           GKE+ +KE+  L   + F G K+
Sbjct: 334 GKERTEKEFEALAKASGFKGIKV 347

[Close]

BLAST of MC00g0349.1 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 102.1 bits (253), Expect = 9.5e-22
Identity = 91/330 (27.58%), Postives = 152/330 (46.06%), Query Frame = 0


Query: 24  INFM--ALKCVVELDIPDAIARH---GHRPMPFSHHVSALKLHPHNSQYLHRSRRILDHY 83
           +NF+   +K   ELD+ + +A+    G    P      A   +PH    + R  R L  Y
Sbjct: 25  LNFVPYIVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAY 84

Query: 84  GFFT----KYKNGKEEEAYSLTN-SSRLLFNHNHSSLSPLLLSLLDSFLLQPWQLLSAWF 143
              T    K + G+E  AY L     +L+ + +  S++P +L+         W  L+   
Sbjct: 85  SVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAI 144

Query: 144 YSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQYKNMFVGVKSLV 203
                  ++ A+    +E+          FN +M N   +V   +L  Y   F GV   V
Sbjct: 145 QEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFV 204

Query: 204 DVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMFQSIPL-PIVMSF 263
           DVGG +G     I   +   + +  DL H++    + H + +  G+MF  IP   +++  
Sbjct: 205 DVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMK 264

Query: 264 DIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGDDMFIETQLFWDML 323
            I+HDW++ +CVEILK CK A+    + G++I+I+M+  ++      D+  +  L  D+ 
Sbjct: 265 WILHDWNDEKCVEILKNCKKALP---ETGRIIVIEMIVPREVSE--TDLATKNSLSADLT 324

Query: 324 MMA-NVGGKEQDKKEWVHLLHKAEFGGHKI 342
           MM+   GGKE+ KKE+  L  +A F   KI
Sbjct: 325 MMSLTSGGKERTKKEFEDLAKEAGFKLPKI 348

[Close]

BLAST of MC00g0349.1 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 99.0 bits (245), Expect = 8.0e-21
Identity = 90/330 (27.27%), Postives = 151/330 (45.76%), Query Frame = 0


Query: 24  INFM--ALKCVVELDIPDAIARH---GHRPMPFSHHVSALKLHPHNSQYLHRSRRILDHY 83
           +NF+   +K   ELD+ + +A+    G    P      A   +PH    + R  R L  Y
Sbjct: 25  LNFVPYIVKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAY 84

Query: 84  GFFT----KYKNGKEEEAYSLTN-SSRLLFNHNHSSLSPLLLSLLDSFLLQPWQLLSAWF 143
              T    K + G+E  AY L     +L+ + +  S++P +L+         W  +    
Sbjct: 85  SVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWYNVQHAI 144

Query: 144 YSHDRIPFKMAHGKQFWEHTASKPQDGDTFNAAMANDVRLVTSVLLGQYKNMFVGVKSLV 203
                  ++ A+    +E+          FN +M N   +V   +L  Y   F GV   V
Sbjct: 145 QEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFV 204

Query: 204 DVGGGIGILARVIAEAFLQTQCVVLDLLHVLTNLEESHNLIYPEGEMFQSIPL-PIVMSF 263
           DVGG +G     I   +   + +  DL H++    + H + +  G+MF  IP   +++  
Sbjct: 205 DVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMK 264

Query: 264 DIMHDWSNGQCVEILKKCKGAITGNGKKGKVILIDMVGNKKGGRDGDDMFIETQLFWDML 323
            I+HDW++ +CVEILK CK A+    + G++I+I+M+  ++      D+  +  L  D+ 
Sbjct: 265 WILHDWNDEKCVEILKNCKKALP---ETGRIIVIEMIVPREVSE--TDLATKNSLSADLT 324

Query: 324 MMA-NVGGKEQDKKEWVHLLHKAEFGGHKI 342
           MM+   GGKE+ KKE+  L  +A F   KI
Sbjct: 325 MMSLTSGGKERTKKEFEDLAKEAGFKLPKI 348

The following BLAST results are available for this feature:

BLAST of MC00g0349.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6VJS4	3.2e-83	47.69	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
B0ZB57	2.8e-79	47.32	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
A0A0N9HMN6	2.2e-68	43.48	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]
B0EXJ8	4.0e-65	40.46	Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 ...	[more]
Q93WU3	8.2e-63	40.23	Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1	[more]

BLAST of MC00g0349.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156551.1	2.60e-182	76.49	probable O-methyltransferase 3 [Momordica charantia]	[more]
XP_022156562.1	2.65e-176	99.60	trans-resveratrol di-O-methyltransferase-like [Momordica charantia]	[more]
XP_022156559.1	8.71e-172	72.13	trans-resveratrol di-O-methyltransferase-like [Momordica charantia]	[more]
XP_023548392.1	1.49e-130	58.57	probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022135671.1	4.00e-129	57.76	probable O-methyltransferase 3 [Momordica charantia]	[more]

BLAST of MC00g0349.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVA5	1.26e-182	76.49	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111023429 PE...	[more]
A0A6J1DSB1	1.28e-176	99.60	trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1DTU5	4.22e-172	72.13	trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1C1F3	1.93e-129	57.76	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE...	[more]
A0A6J1JX64	4.26e-128	57.64	probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111488285 PE=3 ...	[more]

BLAST of MC00g0349.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	4.7e-37	32.66	O-methyltransferase family protein	[more]
AT4G35150.1	5.0e-31	29.82	O-methyltransferase family protein	[more]
AT5G54160.1	4.1e-25	30.03	O-methyltransferase 1	[more]
AT1G51990.1	9.5e-22	27.58	O-methyltransferase family protein	[more]
AT1G51990.2	8.0e-21	27.27	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 2..346 e-value: 4.6E-88 score: 292.7
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 5..344
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 17..346 score: 54.876461
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 104..346 e-value: 2.5E-56 score: 192.5
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 27..345 e-value: 1.4E-55 score: 185.5
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 5..344
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 3..102 e-value: 1.4E-30 score: 107.0
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 27..70 e-value: 2.1E-8 score: 34.0
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 126..336 e-value: 2.7E-37 score: 128.2
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 107..345
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 6..106

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC00g0349	MC00g0349	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC00g0349.1-cds	MC00g0349.1-cds-scaffold7:157068..157343	CDS
MC00g0349.1-cds	MC00g0349.1-cds-scaffold7:157345..157363	CDS
MC00g0349.1-cds	MC00g0349.1-cds-scaffold7:157705..158447	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC00g0349.1	MC00g0349.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity