Lsi10G002330.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G002330.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionheavy metal atpase 1
Locationchr10: 3799223 .. 3809540 (-)
Sequence length2716
RNA-Seq ExpressionLsi10G002330.1
SyntenyLsi10G002330.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAAAAAGTCAATAAGAATCAAATTTTTATAAAGAGGTCACTCGTATAAAAATTCCTCTAATTACGTCATTTTAAACAATTTTTCTCCTCAACCTCACAAAGGAAGCTGCAGTACCCGAAATGATCCCACAATCACGCCGCCGTGAAATTCCGGCATTACCCATGTCCTGGTAACCACGATTTATTCATCTCCTCCACAGATTAGGGCTCGAATTATCCGTTCATAGCATTTCAATGGCGATTATGGACACTCTCGTTGTCCCCATATCCTTGTCCGAACCTCCTCGCCCTTTACTCACCCGCAACACCAACGCCGTTGCTCAATGGAGGATTGGACCTGTTTCCCGCTTCTCGCCGCGTTCTTCTCTAATTCGGCTCAACCGTTTTCCCATTTGCTATAAGAAGCTTTCCTATCCTCGCTTCCGTCACTTCCGTTGCGCCGCCGAGGCCTCCGATCGCCGACACAGTCACCATCACGGCCATCATCACGGCCATCATGGCTGCCAGCACCATTCCAGTGGTGACGGTGATGGAGTGGAGCTGACTGGGGCTCAGAAGGCGTTTGTTCGATTTGCCGACGCTATTAGGTGGACGGACTTGGCGAATTATTTGAGGGAGCATCTACACTTGTGTTGCGGTTCCGCGGCTTTGTTTGTGACTGCGGCTGCGTGTCCTTACTTGGTGCCAAAACCTGCTGTGAAGCCTCTACAGAATGTTTTCATTGCCGTTGCTTTCCCTTTAGTTGGGGTACGAGTGGTTCAAACAACTCACCTTTTCGAACTGCAGCTTGACTGTTATTAATTTTATTGAACGATATTTATGCCTTTGGATGTAAAAGAATCATATCTCTTCTAAGGGTATATGTATTTTAACATGGGATCATGGAGGTGACTCTTAGTTTGGATTCTCATAGTCATTGTCTACTTCTTACCAAAAATTCTCACTTCTTGAGGATTGCAATTATGATAATTGTCCAAATCTAAATTTGAGAATCAATTTTTCTTAATTTTCATGTGACTTGATTAACTTATTTCTAATATATCAGTTTAAACTATTGGGATTAGTAGTAATTCAGCATAGTAAACTTTTTCATTCCTGAAACCAAGTAATACATAACTGTAGTTGTAGAGGGTTGATTCCAAGATATGCTATTTTGTTAAAGCTATTTGCTGTTTGAATATTCAAGAAAATTAATGATCTTAATTATCTTTGAGATTTATGAACTTTCCTTTTACTACATTGAGGATGCATATTCAATCAAATGTTAAATCGTCAGTTTTATGTTGCATTGTCTTGAGTAAGTAAGATGAAACATTCTCTTTCAGGATTCTGGAAATATAAAAAATCCATGTCTCAAGTAGATAGATTAATTTGGCCCCTCTGCCATCAGGTTTGTGGCTTCTCTAGTTGGCAAGCACACACTAACATTCTCTTTTCCTTTTTAGTTCTAAACTTTTGTTCGGTACTTCTCTGTTTGGCAGCCTATCAGAAATTATGGTTATTCAAGGAAATGTATTTTAGGGCACCATGATTTTATTTAGTATTTGAAGATAACTACTGCAGGCTGTTGAGTATTTTCATCATTGTAAGTTTTAAAGTTACTCAGTAATGTGCCAGTAAATCGAATTTTTATGCCACTGTATCAGGTCTCTGCTTCTCTTGATGCTCTTACTGATCTTAGTGGTGGGAAAGTAAACATCCATGTACTAATGGCTCTTGCAGCATTTGCATCAATATTTATGGGGAACCCCTTGGAAGGAGGACTACTTCTTGTGATGTTTAATATGGCTCATATTGGTAAGCAGTTATGAAGAAACAAAATTGCCGACAATTGATATCTTTACTGAGAAGAATCTCATTTTCTTTTTGTTAGTGCAGCTGAAGAGTATTTCACAAAGCAATCAATGATTGATGTTAAGGAGTTGAAGGAAAGTCATCCAGATTTTGCTCTTGTTCTTGATGTGAATGATGATAACCTTCCCGATACTTCAGATCTGGAGCATAGAAGGGTGCCAGTGCGTGATGTTGAAGTGGGCTCTTATATCTTGGTGGGAGCTGGTGAGGTATGTCATAGAAAACTTCCTCCTCATGTTAATATGGTTTGGCAGATGACAATGCAGCCTTTGATCCTGAGATTGATAATGACAATTGATGATAAAGTATTGTTAGTCTTGTATTATTTAAAACCGGTGTACAATCTAGTTACACTTTGACACATGTCATTCCAACACCATAATTCCAACAGAATTTTGTAATCTTTTTGTATCTATTTAGTGAAAACCTGATTGTGTTTTTAAAAGGAGAGTGAACAGCTGATTTGAGATATTAAGAAAAATAGTTCCTTTCTTTTATTTGTGCTTTCTGAGTACTTCGAGAGAAACAATATTCTTCAGTAACTTTTTTCAAATCATATTAAATTTTATAACCTCTAAAATGAATGGGCTATTACTGAGTGACCGACTTATTTTATTTTGTTTCGTATTTTTTCCCACTTTTGTTTTTTTATTATTCTAGGAATGTGATTATGACGTCGTAATCCATCTTGGATATATGGTCATTAATGGTTTTCCAGCTCACTTAACCTAAGTTCCAGTATTGGATTCAACTCAACATAACTTACAATATTTATTGAAAGGAAAATATTTTTACTATTGCCAAATACATTTTTCGATACCCAAGTGTGATAGTTCATAGTGGTTAATACAAAAATTTAATAAAGATCCTTCATAAGAAAAATAATAAATAATGAATCCTAACTATTAAAGACAATTATCTCCTTTTAGAATTCCTAAAAAAATTTCCGGTTCATATGATATTCCTATATACCTCCTGTTGTTGTTGTTGTTCTTATTTTTCTTTTTTGTTATTTTTTGTCTTTTTCTTAATGTGTATTTTAAACTATTCAACTGGTCCTAGATGATGCATTTGTAAATCATAATGAGGGCTGCTTTTATATTAGTCTGTCCCTGTAGATTGTGAAGTTTATCAAGGTTGTGCTACGATCACTGTCGAGCACTTGACAGGAGAAGTCAAACCCTTGCAAATTAAGGTTGGGGAAAGAGTTCCAGGTGGAGCAAGAAACTTGGACGGTAGGATCATAGTGAAGGTATGTCAAGATTGCCTAGTTTATTCCCTCTTAAATGCCTGGTAACATTCTGCATAGGATACCTTGATCTCTTAACTGTAGTTACTTTTTAAACTTGTACTATCTGATACTGTCGTTGTTATAAAATCTCAATTGTACTTGGAAATTAATGCTAAATCTTATCATTTATTAGAATGGTTAATTATTGCCTGTGTTAAACAAAGGGTTTAAGGTGCTCCAAAAAAAAAAAAAAAAAGGAGGTTTTAAGGCATATGAATCCTCGTGAATTACCTTTAATTTTGGGTATCCCAAATGCTAATGAGTTCATAAAAGCTTAGATTGATCAGAATCTGTGAAGTATGAATTTGGTATAGGCTACAAAAGCGTGGGAAGAGTCAACACTTAGCAGGATTGTGCAGTTGACTGAAGAAGCACAACTTAATAAACCAAAGCTACAGAGGTGGCTCAACGAATTTGGTGAGAATTACAGCAAGGTTGTTGTAGTCTTATCCGTAGCTGTTGCCCTCATTGGACCATTATTGTTCAAGTGGCCATTTATATGTACACCAGGTAATCTATGCTATGTGTCTGACTTTAACGTTTTTATTCGGGAGAATTATTTCAGTTTAGGACAAAACTAAGGCTTTTATGCATTTCCAAACTAGATCAGCCTATTGTCTTTCCTTCTTGAGGACAGTAAACTAGACTTTAGTCATCACCAATAAGAATATTTGAAAGTTGAGCTCAGCTCAAATTAATTGACAACTTAATATACCAGCCAGTTTTTCTGTGCCTCCCTACGTGTGTTGGCCTTGGAAAGTTGAGCTCAGCTCACCCTGTCTCATTCTCTCTCTCTCTCTCTGGTGTCTGTTTCACTTCTGATGCCTGTATAATCTTTAGGTTTCAGAGGATCAGTTTATAGAGCACTTGGGCTTATGGTAGCCGCATCACCATGTGCACTGGCAGCAGCACCATTGGCATATGCTATTGCGATAAGCTCCTGTGCAAGAAAGGTATTTATTATTATCACCTGGTGATATAGTTATGTACTAATTGTCTGATATGAACATCAGAAGTTGGTGAACACATATGTATTATTGGGTCCATTACACTATCACAGAAACCAACGAAGAAATCCATTTTTTATGAAAATTATACTCGTGCAACAACCTTTTATGTCATTAAATCATTTAAGTATGATAAGTATGTTGATTTCTTTACATGTTATCATTTAAGCTTCGCTGTCATGGTCAATCCAGTTACATGTTATGATCTGAGAATGTTGCTTAACCCTGTATATGTATGTATGTATAAATTTGAAATGGAAACCATATTATGAATTTGGTTGTTATGGTTGAATGATTGAGTGGCTATTTAGCTCTCTCTATATATATCAAAAAAAAAGGTTAGGTGCACTTGCACATAGAGAGAGATATCACAGCAATTAACTCTAAACTCATCAACTTGCATGCTAAACCGAAATATTGTCTTCCATGGATGCTTACTTCATTCTTTCACATTTCGCTCGAAGATTTAGCTATTTAGCTCTCTCTCTATAAAAAAAGTTTAGGTGCACTCACACATGGAGAGAGATATCACAGCAATTAACTCTAAACTCATCAACTTGCATGCTAAACCGAAACATTGTCTTCAGTGGATGCTTACTTCAATCTTTCACATTTCGCTTGAAGATTTAGCAAGTATTTCGATCATGTACAAAGATACTTTTATTTGCTAATAGCATGAAAAAATGGACATGTGCTTGATGGAACAGGGGATACTCCTGAAAGGTGGACACGTGCTTGATGCTATGGCTTCTTGTCACACTGTTGCATTTGATAAAACCGGAACATTGACTACAGGAGGACTTGTGTTTAAAGCCATAGAACCCATTTATGGGCATAAGATTGGAGGAGATGAATCACATTTTGCTTCCTGTTGCATACCAAGCTGTGAAAAAGAAGCTCTTGCAGTGGCAGCTGCCATGGAAAAGGGTACTACTCACCCAATTGGAAGGTAGTTGTCAAAATGAATTGTCTGACTAGCATATTATACTTCTTATGGGTTTTCTTTCCCTGACAAGGTGTGATATAATTAATCTACTATTATATTTGGCCAAAAACATGCCGACGTCACTTGCCAGACAAAATGTTTTTACCGTTTATTTCATGCTCACCATTTATGTGGCTTCTTACACCTATGTCATGATTTTACCTTCCCGACATTTGATGTTGTATCAACTTAATGATTTTTTCTATGGAACAAAAGAGTTCAACTTTCACTGCATGCTTAGATGGTCAATGTTAAATTTGGGTGAGAGTATTTTCATTCACCGCATAAGATTGATTATATGAATGAATATAAGAATGTCATTATATAACTTGCTTGAGAATTGACGCGCATAAGATTGCTTATATGAATGAATAAGAATGTCATTATATAACTTGCTTGAGAATTGGGACACGGGTTTATATATTTAGAATAACATCCCTGGGCACAAGTATGAACTTGATCCTTTTGGAAGTATCAAGTAAGGAAACTCTTTCACACACCTGCAGTTACTTATGTTGGAAGATGGAAGACCTGGATTAGCGTTATATTTTTAGTATGAATAAGTAGGAACTATTTTTATCTCTTGGGACTGAATGATTAAGGTTCCGTTTGATAACTACTTGGTTTTTGGTTTTTGAAAATAATGCTTGTTTCCTTATCACTTCTTTATCAAGGTTTTCATCTTTCCTAACTAGACACTTGAATTCTTATAGATGTTTTTTTTCTCTCTCAAGAATTGGTTTGGTTTGTGAAAACACTCATGAAAGGTAGATAATGAAACAAAAAAATCAATAGATGGAAATAGTGTTTATAAGCTTAATTTTAAAAAACCAAATGGTTATCGAATTGAGTCGAAATTTTTCATTACTACTAGCAATAGTCCTCCATGTTATTAGCTTCAGTTTCATATATTTTTTCTGTGTGTTGGAAGAAGGCTGGTTAATCGCAGTCACCTTTTATTCTTTCTTAATTATGCAGTTAATGTATTTCAATTAACTCATGCCAGTGTCTCAAATGTGCTGGACAGCTTTTCTTCTGGAGTATTGGCTTCTAATATCTGTCTGGAATATTCATTTTCTAATTCCAAGTAGTTATACCCTTTTCTGTTTTTGGTTGTAGAGCTGTAGTAGATCATAGTGTGGGGAAAGATCTTCCTTCTATTTCTGTTGACAGTTTTGAATATTTCCCTGGAAGAGGTCTCATCGCAACTCTGCATGGCATTAAGGTAGTGTTTGCTTCATATTGCTCGATGTTTTATTGATCTTTGGTTTCATCTTCAACAGTAGGCTTCCTTAGTTTACTTCCTTTTCATTTTTCTACAATAAAGTTCCTTTTCTTGCTGCACTTTTCTATCATCATATATTTTAACCTCCTTCGCTCGTATGCCACAGGCCATAGGGTATCTGATTCCACTCCTCTGAATAAAAATGTGCTATCATCAAACAATATTCTAGCACCATGGTTCTCCGTTTTTCCAAAAAAAAAAAAAGGAGCAACTCAGAAATTTCATCAAGCATCTTGATGTCGGTCTTTCTGAAGGGTTGACTATGCTTCAATAGCAAGAATTGTCATTACTTTTAGATGTCAAATTAGAAATGGTCTTCTCATTTTAGAATAGCTGGTCAACTTTGGTCAAAGTTAGATTTTCGTATTTTACCCTTGAAAACTCACAAGATTTTGGAAATGCTTAGGCGTTAGATTGTAAGCAAGATGAAGCAATAACTTCTGTACATGAATGTTTGAAATTCTAGATTTATATCCGCACATGAGATCACCTACATGTCTAAAAACTAATAAATTACAAATCTGTTGAAGGTTTATTATTTCTTTCTTTTTGGTATCCATGAATGTTCGAGCCAGGTTACGTGCATCTCGACTAATTTTACGAGACAATTCACATGATCCTACAACATTTGGGTGTCAAGGAAACTCGTAGGATATTAAATCCTATGTAGATGACCACTATGGATTGAACCCATGACTTTTTAGCCCTTTATCGAGGTCATGCCCCCTATTTACTACTAGCCCTTTATCTATTGAAGGTTAAATGAAACTTCATCTACTTCAAATCAATAATTGTTCAACACCCTGCCAAATGTCCAATCCAATGAAAACAACCATATATGAAATATCCTAGTTTGCCTCAAGTGAAGTTATTTTGAAAAAGAGCTGGAGTAAAACCTACCATCATAGAATCTATCATTATTCACAACTTGAAGGGAACTGTTTCTTCTTTCCAAAATGCACCTTGTCACACTCTGGCTGATTTCGATTAGGCTTTTTTGAAATTTGTAACATCTGAATCGTAGTAAAAACTTTGCTATTAACACTTTTGTTTTTACTTATTTAGGGTGTATTTGGTCCAAGGAGTTTGAAAGTAGGAGTGGGGAAGTAGGGAGTTGTGAACTCCACTTCTTGTTTGGCCCAAGGAGTTAGTGGGTCCCACCACTAAAGTTTAAAATTTTAAAACTCTTAGTTCAATAATTGCCATTAGGTAGAAGGAGAAGAGTATTTACATTCAATCTCTATCTTACAGTCAGGAATTGGAGGTAAATTGCGGAAAGCATCTCTTGGTTCTGTGGATTTCATCACTTCCTTCTGCAAATCCGAAAATGAATCGAGGAAGATCAAGGATGCTGTTAGAACATCAACATATGGAAGTGAATTTGTTCATGCTGCTCTTTCAGTTGATCAAAAGGTTTGAATTTTGACGATCATCCAAAGTTAGGCGGATACTTTTAAACACATGAAATCATATTTGCATAAATTTTACAAATATTATCGTTAATCACTAATTAATTGAAATATTTTATTATATAGGTATCGATATGCTTGATGGGTACCGTTCCTCTAGTTCAGTCCGTCCATTAACTTTATGCCTGTGTATTTTCTTGTGCACTGCATTTTGACTTCACTACATTTTGAAATTTTCATCTTTGCAAAAACGATATGCAACTAGCCTCATTAGAAAGCAAGACTGAGTGATTCTTCTTTTCCATATTATTATTTGAATATTCTTGATGGGACTTTTAATCAGTAGGTTTGTCATGCTTTGAGAACGTATCTTGCACTTCTATTATTGTCTCAATGACACAGTAAACGATAGTATGTTGAGTTTTTGCTACATCAATGCAGGTAACACTGATTCACCTTGAGGATCAACCTCGACCTGAAGTTGTAGATGCAATAGCAGAACTACGAGATAAGGGAAAACTCCGAGTTATGATGTTAACAGGTGATCATGATTCAAGTGCATGGAAAGTTGCGAATGCTGTGGGCATAAATGAAGTTTACTACAGTCTTAAGCCAGAGGACAAACTTGCTCACGTGAAGGAAATTTCAAGGGAGATGGGTGAGACTTCTTACTCAGTCATTTCATGTTTGCTTTTTGTATAAACCGTACTTTATGTTAATTGCCAATATTCCTGAATTCTCATCACATCTTAGTTTCCTGTCGAGATAAAGATGCTCCCTACTTAATCTTCTAGGATCCATGTTAATTTCCACAAGTTGATCCTTGGTGAGATTTCTTTTTACCCATTTGTTTCAAGACAGATTTTGTGTGTCTGAAATGTAGCCAATATATGATCGATTTCTTCCGTTACATTCATTTTTACCCACCAAGAAATTGCAGGATTTTATGTTTTCAGGCCATTGTTTTTATTTTTCTTAGCGAGTAGTGAAGTATAAAACTATTGAGACCTGATTCTATGCACGCTGGATGGACGATTATATGTTAATGTGTCAGTAGCCATACTATGTGAGCTTTATATTAATTTTACGAATCTTCAGAGTTTATTGGTTCAATATTATTGATCTATTCTGTGATTTTTGCTCTGAATATCAACAATTTAACTTTACTTATCATCCTAATATCTTCACTGAATATGGATGGTAAACCAATGTGATTGATCATCTCGATTGAATATATTTCTCTCATATTCTAAAGCTGGAATTATACTTTGGTTGTTAGAAGAGCAATATTGTTACTGCTCTCCTTTGAATGAACTCTCTTCCACTTCTAAGTTTTATACTTTGCCAAATGATTGATTGATTGGCCAGCAACCATCCCTTTTCTAGGAGTTAAATAATGAGCACTCTATCTCATTTTGTTCTAGGGGGTGGCCTGATCATGGTAGGTGAAGGTATAAACGATGCACCTGCACTTGCTGCCGCTACTGTGGGAATAGTACTTGCCCATCGTGCAAGTGCTACTGCTACAGCTGTTGCAGACGTCCTGCTTCTTCAGGACAGTATTTCCGGTGTACCGTTCTGCATTGCCAAATCTCGTCAGACCACTTCACTGGTATTTTTGTTTGCCATATATTTTTGGTTATGGTACTTGATTTTCTTTTCCTGTTTCACTATTTGAATTTGAAGGCGTAATTGTCATTTCAGGTCAAGCAAAATGTGACCCTTGCCTTGTCATCTATCCTTCTAGCCTCTCTCCCATCCGTTTTGGGGTTTCTTCCCTTGTGGCTAACGGTATGAAGTTAAATGCTTACTGATTTATTCCGTGAACTTAATTAGTGCCCAGTTATTAAATTTTATCCCGTGGATTTTATGAGTGGTAGACCGATCTAAAATCAGCAAGTTTGTGGTTGTTGAGAACGCGAGGAAGCTCCTCCTATTTTCTAGTCATGATTATAAATTAGATCATTTTGGTTTGTGAATTAGGCTGGTGATATCTATATGTTTTTAACACAATTTCAAACGCCCTTATGATTTGCAAATTTTAAAAACTACATAGTGGCATAAAACAGCCAAAGCGAGTTTAGCTCAATGGTAATTGGCATGACCTTCTTTCCTAGAGGTCGAAGATTCAATTTTCCATCTCTGAAGTTGTACTAAAAGAAAAAATATAGTTGCATAAAACACTTGATATCATCTACAAAATCTCGTGCTTTCGGAAATCTTTTCTCCATATAGTTCTCCATTTGCACAGTTGCAACCATTCCATTTAACCAAGTGATTTTCATTTCATGATTCTCTATTCATAAATGCAGGTCCTTCTACACGAGGGTGGCACTCTCCTGGTTTGCCTGAACTCCATACGTGCTCTAAATCAGCCCTCATGGTCATGGAAACAAGATTTTCAAAATCTGATATGCGATGCCAGATCTAAACTCAACACGACACCCGAAACTAGCTCTGGTACCATCCGAACTGCAACTTCATAG

mRNA sequence

GCAAAAAAGTCAATAAGAATCAAATTTTTATAAAGAGGTCACTCGTATAAAAATTCCTCTAATTACGTCATTTTAAACAATTTTTCTCCTCAACCTCACAAAGGAAGCTGCAGTACCCGAAATGATCCCACAATCACGCCGCCGTGAAATTCCGGCATTACCCATGTCCTGGTAACCACGATTTATTCATCTCCTCCACAGATTAGGGCTCGAATTATCCGTTCATAGCATTTCAATGGCGATTATGGACACTCTCGTTGTCCCCATATCCTTGTCCGAACCTCCTCGCCCTTTACTCACCCGCAACACCAACGCCGTTGCTCAATGGAGGATTGGACCTGTTTCCCGCTTCTCGCCGCGTTCTTCTCTAATTCGGCTCAACCGTTTTCCCATTTGCTATAAGAAGCTTTCCTATCCTCGCTTCCGTCACTTCCGTTGCGCCGCCGAGGCCTCCGATCGCCGACACAGTCACCATCACGGCCATCATCACGGCCATCATGGCTGCCAGCACCATTCCAGTGGTGACGGTGATGGAGTGGAGCTGACTGGGGCTCAGAAGGCGTTTGTTCGATTTGCCGACGCTATTAGGTGGACGGACTTGGCGAATTATTTGAGGGAGCATCTACACTTGTGTTGCGGTTCCGCGGCTTTGTTTGTGACTGCGGCTGCGTGTCCTTACTTGGTGCCAAAACCTGCTGTGAAGCCTCTACAGAATGTTTTCATTGCCGTTGCTTTCCCTTTAGTTGGGGTCTCTGCTTCTCTTGATGCTCTTACTGATCTTAGTGGTGGGAAAGTAAACATCCATGTACTAATGGCTCTTGCAGCATTTGCATCAATATTTATGGGGAACCCCTTGGAAGGAGGACTACTTCTTGTGATGTTTAATATGGCTCATATTGCTGAAGAGTATTTCACAAAGCAATCAATGATTGATGTTAAGGAGTTGAAGGAAAGTCATCCAGATTTTGCTCTTGTTCTTGATGTGAATGATGATAACCTTCCCGATACTTCAGATCTGGAGCATAGAAGGGTGCCAGTGCGTGATGTTGAAGTGGGCTCTTATATCTTGGTGGGAGCTGGTGAGTCTGTCCCTGTAGATTGTGAAGTTTATCAAGGTTGTGCTACGATCACTGTCGAGCACTTGACAGGAGAAGTCAAACCCTTGCAAATTAAGGTTGGGGAAAGAGTTCCAGGTGGAGCAAGAAACTTGGACGGTAGGATCATAGTGAAGGCTACAAAAGCGTGGGAAGAGTCAACACTTAGCAGGATTGTGCAGTTGACTGAAGAAGCACAACTTAATAAACCAAAGCTACAGAGGTGGCTCAACGAATTTGGTGAGAATTACAGCAAGGTTGTTGTAGTCTTATCCGTAGCTGTTGCCCTCATTGGACCATTATTGTTCAAGTGGCCATTTATATGTACACCAGGTTTCAGAGGATCAGTTTATAGAGCACTTGGGCTTATGGTAGCCGCATCACCATGTGCACTGGCAGCAGCACCATTGGCATATGCTATTGCGATAAGCTCCTGTGCAAGAAAGGGGATACTCCTGAAAGGTGGACACGTGCTTGATGCTATGGCTTCTTGTCACACTGTTGCATTTGATAAAACCGGAACATTGACTACAGGAGGACTTGTGTTTAAAGCCATAGAACCCATTTATGGGCATAAGATTGGAGGAGATGAATCACATTTTGCTTCCTGTTGCATACCAAGCTGTGAAAAAGAAGCTCTTGCAGTGGCAGCTGCCATGGAAAAGGGTACTACTCACCCAATTGGAAGAGCTGTAGTAGATCATAGTGTGGGGAAAGATCTTCCTTCTATTTCTGTTGACAGTTTTGAATATTTCCCTGGAAGAGGTCTCATCGCAACTCTGCATGGCATTAAGTCAGGAATTGGAGGTAAATTGCGGAAAGCATCTCTTGGTTCTGTGGATTTCATCACTTCCTTCTGCAAATCCGAAAATGAATCGAGGAAGATCAAGGATGCTGTTAGAACATCAACATATGGAAGTGAATTTGTTCATGCTGCTCTTTCAGTTGATCAAAAGGTAACACTGATTCACCTTGAGGATCAACCTCGACCTGAAGTTGTAGATGCAATAGCAGAACTACGAGATAAGGGAAAACTCCGAGTTATGATGTTAACAGGTGATCATGATTCAAGTGCATGGAAAGTTGCGAATGCTGTGGGCATAAATGAAGTTTACTACAGTCTTAAGCCAGAGGACAAACTTGCTCACGTGAAGGAAATTTCAAGGGAGATGGGGGGTGGCCTGATCATGGTAGGTGAAGGTATAAACGATGCACCTGCACTTGCTGCCGCTACTGTGGGAATAGTACTTGCCCATCGTGCAAGTGCTACTGCTACAGCTGTTGCAGACGTCCTGCTTCTTCAGGACAGTATTTCCGGTGTACCGTTCTGCATTGCCAAATCTCGTCAGACCACTTCACTGGTCAAGCAAAATGTGACCCTTGCCTTGTCATCTATCCTTCTAGCCTCTCTCCCATCCGTTTTGGGGTTTCTTCCCTTGTGGCTAACGGTCCTTCTACACGAGGGTGGCACTCTCCTGGTTTGCCTGAACTCCATACGTGCTCTAAATCAGCCCTCATGGTCATGGAAACAAGATTTTCAAAATCTGATATGCGATGCCAGATCTAAACTCAACACGACACCCGAAACTAGCTCTGGTACCATCCGAACTGCAACTTCATAG

Coding sequence (CDS)

ATGGCGATTATGGACACTCTCGTTGTCCCCATATCCTTGTCCGAACCTCCTCGCCCTTTACTCACCCGCAACACCAACGCCGTTGCTCAATGGAGGATTGGACCTGTTTCCCGCTTCTCGCCGCGTTCTTCTCTAATTCGGCTCAACCGTTTTCCCATTTGCTATAAGAAGCTTTCCTATCCTCGCTTCCGTCACTTCCGTTGCGCCGCCGAGGCCTCCGATCGCCGACACAGTCACCATCACGGCCATCATCACGGCCATCATGGCTGCCAGCACCATTCCAGTGGTGACGGTGATGGAGTGGAGCTGACTGGGGCTCAGAAGGCGTTTGTTCGATTTGCCGACGCTATTAGGTGGACGGACTTGGCGAATTATTTGAGGGAGCATCTACACTTGTGTTGCGGTTCCGCGGCTTTGTTTGTGACTGCGGCTGCGTGTCCTTACTTGGTGCCAAAACCTGCTGTGAAGCCTCTACAGAATGTTTTCATTGCCGTTGCTTTCCCTTTAGTTGGGGTCTCTGCTTCTCTTGATGCTCTTACTGATCTTAGTGGTGGGAAAGTAAACATCCATGTACTAATGGCTCTTGCAGCATTTGCATCAATATTTATGGGGAACCCCTTGGAAGGAGGACTACTTCTTGTGATGTTTAATATGGCTCATATTGCTGAAGAGTATTTCACAAAGCAATCAATGATTGATGTTAAGGAGTTGAAGGAAAGTCATCCAGATTTTGCTCTTGTTCTTGATGTGAATGATGATAACCTTCCCGATACTTCAGATCTGGAGCATAGAAGGGTGCCAGTGCGTGATGTTGAAGTGGGCTCTTATATCTTGGTGGGAGCTGGTGAGTCTGTCCCTGTAGATTGTGAAGTTTATCAAGGTTGTGCTACGATCACTGTCGAGCACTTGACAGGAGAAGTCAAACCCTTGCAAATTAAGGTTGGGGAAAGAGTTCCAGGTGGAGCAAGAAACTTGGACGGTAGGATCATAGTGAAGGCTACAAAAGCGTGGGAAGAGTCAACACTTAGCAGGATTGTGCAGTTGACTGAAGAAGCACAACTTAATAAACCAAAGCTACAGAGGTGGCTCAACGAATTTGGTGAGAATTACAGCAAGGTTGTTGTAGTCTTATCCGTAGCTGTTGCCCTCATTGGACCATTATTGTTCAAGTGGCCATTTATATGTACACCAGGTTTCAGAGGATCAGTTTATAGAGCACTTGGGCTTATGGTAGCCGCATCACCATGTGCACTGGCAGCAGCACCATTGGCATATGCTATTGCGATAAGCTCCTGTGCAAGAAAGGGGATACTCCTGAAAGGTGGACACGTGCTTGATGCTATGGCTTCTTGTCACACTGTTGCATTTGATAAAACCGGAACATTGACTACAGGAGGACTTGTGTTTAAAGCCATAGAACCCATTTATGGGCATAAGATTGGAGGAGATGAATCACATTTTGCTTCCTGTTGCATACCAAGCTGTGAAAAAGAAGCTCTTGCAGTGGCAGCTGCCATGGAAAAGGGTACTACTCACCCAATTGGAAGAGCTGTAGTAGATCATAGTGTGGGGAAAGATCTTCCTTCTATTTCTGTTGACAGTTTTGAATATTTCCCTGGAAGAGGTCTCATCGCAACTCTGCATGGCATTAAGTCAGGAATTGGAGGTAAATTGCGGAAAGCATCTCTTGGTTCTGTGGATTTCATCACTTCCTTCTGCAAATCCGAAAATGAATCGAGGAAGATCAAGGATGCTGTTAGAACATCAACATATGGAAGTGAATTTGTTCATGCTGCTCTTTCAGTTGATCAAAAGGTAACACTGATTCACCTTGAGGATCAACCTCGACCTGAAGTTGTAGATGCAATAGCAGAACTACGAGATAAGGGAAAACTCCGAGTTATGATGTTAACAGGTGATCATGATTCAAGTGCATGGAAAGTTGCGAATGCTGTGGGCATAAATGAAGTTTACTACAGTCTTAAGCCAGAGGACAAACTTGCTCACGTGAAGGAAATTTCAAGGGAGATGGGGGGTGGCCTGATCATGGTAGGTGAAGGTATAAACGATGCACCTGCACTTGCTGCCGCTACTGTGGGAATAGTACTTGCCCATCGTGCAAGTGCTACTGCTACAGCTGTTGCAGACGTCCTGCTTCTTCAGGACAGTATTTCCGGTGTACCGTTCTGCATTGCCAAATCTCGTCAGACCACTTCACTGGTCAAGCAAAATGTGACCCTTGCCTTGTCATCTATCCTTCTAGCCTCTCTCCCATCCGTTTTGGGGTTTCTTCCCTTGTGGCTAACGGTCCTTCTACACGAGGGTGGCACTCTCCTGGTTTGCCTGAACTCCATACGTGCTCTAAATCAGCCCTCATGGTCATGGAAACAAGATTTTCAAAATCTGATATGCGATGCCAGATCTAAACTCAACACGACACCCGAAACTAGCTCTGGTACCATCCGAACTGCAACTTCATAG

Protein sequence

MAIMDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRFRHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS
Homology
BLAST of Lsi10G002330.1 vs. ExPASy Swiss-Prot
Match: Q9M3H5 (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HMA1 PE=2 SV=2)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 571/794 (71.91%), Postives = 649/794 (81.74%), Query Frame = 0

Query: 32  RIGPVSRFS--PRSSLIRLNRFPICYKKLSYPRFRHFRCAAEASDRRHSHHHGHHHGHHG 91
           R+  V+ FS  P  +L+R     I       PR    R      D  H HHH     HH 
Sbjct: 25  RLARVNSFSILPPKTLLRQKPLRISASLNLPPRSIRLRA---VEDHHHDHHHDDEQDHHN 84

Query: 92  CQHHSSGDG-DGVEL---TGAQKAFVRFADAIRWTDLANYLREHLHLCCGSAALFVTAAA 151
             HH    G   VEL   +  QK    FA AI W  LANYLREHLHLCC +AA+F+ AA 
Sbjct: 85  HHHHHHQHGCCSVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFLAAAV 144

Query: 152 CPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAAFASIFMGN 211
           CPYL P+P +K LQN F+ V FPLVGVSASLDAL D++GGKVNIHVLMALAAFAS+FMGN
Sbjct: 145 CPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASVFMGN 204

Query: 212 PLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRR 271
            LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL+++V++ N+P+ SDL ++ 
Sbjct: 205 ALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDLSYKS 264

Query: 272 VPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNL 331
           VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVKPL+ K G+RVPGGARNL
Sbjct: 265 VPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGGARNL 324

Query: 332 DGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIG 391
           DGR+IVKATKAW +STL++IVQLTEEA  NKPKLQRWL+EFGENYSKVVVVLS+A+A +G
Sbjct: 325 DGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAIAFLG 384

Query: 392 PLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGGHVL 451
           P LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGILLKG  VL
Sbjct: 385 PFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGAQVL 444

Query: 452 DAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAA 511
           DA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   +CCIP+CEKEALAVAAA
Sbjct: 445 DALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEKEALAVAAA 504

Query: 512 MEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKS-GIGGKLRKASLG 571
           MEKGTTHPIGRAVVDHSVGKDLPSI V+SFEYFPGRGL AT++G+K+     +LRKASLG
Sbjct: 505 MEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRKASLG 564

Query: 572 SVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIA 631
           S++FITS  KSE+ES++IKDAV  S+YG +FVHAALSVDQKVTLIHLEDQPRP V   IA
Sbjct: 565 SIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVSGVIA 624

Query: 632 ELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMV 691
           EL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE GGGLIMV
Sbjct: 625 ELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGGLIMV 684

Query: 692 GEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQN 751
           GEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GVPFC+AKSRQTTSLVKQN
Sbjct: 685 GEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSLVKQN 744

Query: 752 VTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNLIC 811
           V LAL+SI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNS+R LN PSWSWKQD  +LI 
Sbjct: 745 VALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIVHLIN 804

Query: 812 DARSKLNTTPETSS 819
             RS+  T+  ++S
Sbjct: 805 KLRSQEPTSSSSNS 814

BLAST of Lsi10G002330.1 vs. ExPASy Swiss-Prot
Match: Q60048 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes OX=1639 GN=cadA PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.2e-73
Identity = 198/682 (29.03%), Postives = 348/682 (51.03%), Query Frame = 0

Query: 113 FADAIRWTDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGV 172
           F D   +TD  +++R++  L    + LF+       ++          +FI   F + G 
Sbjct: 72  FTDPEHFTDHQSFIRKNWRLLL--SGLFIAVGYASQIMNGEDFYLTNALFIFAIF-IGGY 131

Query: 173 SASLDALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMI 232
           S   +   +L   +  +  LM +A   + F+G   EG +++++F ++   E Y   ++  
Sbjct: 132 SLFKEGFKNLLKFEFTMETLMTIAIIGAAFIGEWAEGSIVVILFAVSEALERYSMDKARQ 191

Query: 233 DVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVY 292
            ++ L +  P  ALV     D          R V V D+++G  +++  G+ + +D  V 
Sbjct: 192 SIRSLMDIAPKEALVRRSGTD----------RMVHVDDIQIGDIMIIKPGQKIAMDGHVV 251

Query: 293 QGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEA 352
           +G + +    +TGE  P++  + + V  G  N +G + V  TK  E++T+S+I+ L EEA
Sbjct: 252 KGYSAVNQAAITGESIPVEKNIDDSVFAGTLNEEGLLEVAVTKRVEDTTISKIIHLVEEA 311

Query: 353 QLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVA 412
           Q  +   Q +++ F + Y+  ++V++  +A + PLLF         +   VY+ L ++V 
Sbjct: 312 QGERAPAQAFVDTFAKYYTPAIIVIAALIATVPPLLF------GGNWETWVYQGLSVLVV 371

Query: 413 ASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTG-GLVFK 472
             PCAL  + P+A   AI + A+ G+L+KGG  L+ +     +AFDKTGTLT G  +V  
Sbjct: 372 GCPCALVVSTPVAIVTAIGNAAKNGVLVKGGVYLEEIGGLKAIAFDKTGTLTKGVPVVTD 431

Query: 473 AIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK--DLP 532
            IE      I  ++++               + AA+E+ + HP+  A++ +   +  DL 
Sbjct: 432 YIELTEATNIQHNKNYI--------------IMAALEQLSQHPLASAIIKYGETREMDLT 491

Query: 533 SISVDSFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESR-KIKDAVR 592
           SI+V+ F    G+G+  T+ G    +G  +    L +  F  S  +  ++ + K K A+ 
Sbjct: 492 SINVNDFTSITGKGIRGTVDGNTYYVGSPVLFKELLASQFTDSIHRQVSDLQLKGKTAML 551

Query: 593 TSTYGSEFVHAALSVDQK-VTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAW 652
             T            +QK ++++ + D+ R      I  L + G  + +MLTGD+ ++A 
Sbjct: 552 FGT------------NQKLISIVAVADEVRSSSQHVIKRLHELGIEKTIMLTGDNQATAQ 611

Query: 653 KVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHR 712
            +   VG++E+   L P+DKL ++K++     G + MVG+GINDAPALAAATVGI +   
Sbjct: 612 AIGQQVGVSEIEGELMPQDKLDYIKQLKINF-GKVAMVGDGINDAPALAAATVGIAMGGA 671

Query: 713 ASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPL 772
            + TA   ADV L+ D +  +PF +  SR+T  ++KQN+T +L   L+A L  + G+L L
Sbjct: 672 GTDTAIETADVALMGDDLQKLPFTVKLSRKTLQIIKQNITFSLVIKLIALLLVIPGWLTL 707

Query: 773 WLTVLLHEGGTLLVCLNSIRAL 789
           W+ ++   G TLLV LN +R +
Sbjct: 732 WIAIMADMGATLLVTLNGLRLM 707

BLAST of Lsi10G002330.1 vs. ExPASy Swiss-Prot
Match: P58414 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=cadA PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 4.2e-73
Identity = 184/632 (29.11%), Postives = 328/632 (51.90%), Query Frame = 0

Query: 161 VFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 220
           +   +A  + G +   +   +L      +  LM +A   +  +G   EG +++++F  + 
Sbjct: 117 ILYVIAIVVGGFNLFKEGFANLIKLDFTMESLMTIAIIGASIIGEWAEGSIVVILFAFSE 176

Query: 221 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 280
           + E Y   ++   ++ L +  P  AL+            D   + + V D+++G  +++ 
Sbjct: 177 VLERYSMDKARQSIRSLMDIAPKEALI----------RRDDVEQMIAVSDIQIGDIMIIK 236

Query: 281 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEES 340
            G+ + +D  V +G + I    +TGE  P++ KV + V  G  N +G + VK TK  E++
Sbjct: 237 PGQKIAMDGVVIKGYSAINQSAITGESIPVEKKVDDEVFAGTLNEEGLLEVKVTKHVEDT 296

Query: 341 TLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 400
           T+S+I+ L EEAQ  +   Q ++++F + Y+  ++++++ V ++ PL F   +       
Sbjct: 297 TISKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMLIALLVVVVPPLFFGGDWDTW---- 356

Query: 401 GSVYRALGLMVAASPCALA-AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 460
             VY+ L L+V   PC+L  + P++   AI + A+ G+L+KGG  L+ +     +AFDKT
Sbjct: 357 --VYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLQAIAFDKT 416

Query: 461 GTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 520
           GTLT G  V     P   H                 E+ +L++  A+E  + HP+  A++
Sbjct: 417 GTLTKGKPVVTDFIPYSEH---------------MDEQNSLSIITALETMSQHPLASAII 476

Query: 521 DHSV--GKDLPSISVDSFEYFPGRGLIATLHGIKSGIG-GKLRKASLGSVDFITSFCKSE 580
             ++    D  SI +D+F    G+G+   ++GI   IG  KL ++SL     I+   +S 
Sbjct: 477 SKAMIDNVDYKSIEIDNFSSITGKGVKGEVNGITYYIGSSKLFESSLEKSQSISQTYQSL 536

Query: 581 NESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMM 640
            +  K         +G+E           + +I + D+ R    + IA+L   G    +M
Sbjct: 537 QKQGK-----TAMLFGTE--------SNILAIIAVADEVRESSKEVIAQLHKLGIAHTIM 596

Query: 641 LTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAA 700
           LTGD++ +A  +   +G++++   L PEDKL ++KE+ ++  G + M+G+G+NDAPALAA
Sbjct: 597 LTGDNNDTAQFIGKEIGVSDIKAELMPEDKLTYIKEL-KQTYGKVAMIGDGVNDAPALAA 656

Query: 701 ATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLAS 760
           +TVGI +    + TA   ADV L+ D +  +PF +  SR+T  ++KQN+T +L   LLA 
Sbjct: 657 STVGIAMGGAGTDTALETADVALMGDDLKKLPFIVNLSRKTLKIIKQNITFSLGIKLLAL 703

Query: 761 LPSVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 789
           L  + G+L LW+ ++   G TLLV LN +R +
Sbjct: 717 LLVLPGWLTLWIAIVADMGATLLVTLNGLRLM 703

BLAST of Lsi10G002330.1 vs. ExPASy Swiss-Prot
Match: Q6GIX1 (Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=cadA PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.2e-71
Identity = 177/605 (29.26%), Postives = 318/605 (52.56%), Query Frame = 0

Query: 188 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 247
           ++  LM +A   +  +G   E  +++V+F ++   E +   ++   ++ L +  P  ALV
Sbjct: 160 DMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALV 219

Query: 248 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 307
           +    + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 220 MRNGQEIM----------IHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGES 279

Query: 308 KPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFG 367
            P+   V + V  G  N +G + VK TK  E++T+S+I+ L EEAQ  +   Q ++++F 
Sbjct: 280 VPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFA 339

Query: 368 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 427
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 340 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 399

Query: 428 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESH 487
            AI + A+KG+L+KGG  L+ + +   +AFDKTGTLT      K +  +   K+  D+  
Sbjct: 400 SAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLT------KGVPVVTDFKVLNDQVE 459

Query: 488 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDL--PSISVDSFEYFPGRGLI 547
                    EKE  ++  A+E  + HP+  A++  +   ++    + V+ F    GRG+ 
Sbjct: 460 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRGIQ 519

Query: 548 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQ 607
             + G    IG       L   DF   F   EN+ + +++  +T+          +  DQ
Sbjct: 520 GNIDGTTYYIGSPRLFKELNVSDFSLEF---ENKVKVLQNQGKTA--------MIIGTDQ 579

Query: 608 KVT-LIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 667
            +  +I + D+ R    + I +L   G  + +MLTGD+  +A  +   VG++++   L P
Sbjct: 580 TILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSELMP 639

Query: 668 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 727
           +DKL ++K++  E  G + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D 
Sbjct: 640 QDKLDYIKKMKAE-HGNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 699

Query: 728 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 787
           +S +PF +  SR+T +++K N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 700 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 721

Query: 788 SIRAL 789
           S+R +
Sbjct: 760 SLRLM 721

BLAST of Lsi10G002330.1 vs. ExPASy Swiss-Prot
Match: P20021 (Cadmium-transporting ATPase OS=Staphylococcus aureus OX=1280 GN=cadA PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.5e-70
Identity = 179/606 (29.54%), Postives = 318/606 (52.48%), Query Frame = 0

Query: 188 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 247
           ++  LM +A   +  +G   E  +++++F ++   E +   +S   ++ L +  P  ALV
Sbjct: 161 DMKTLMTVAVIGATIIGKWAEASIVVILFAISEALERFSMDRSRQSIRSLMDIAPKEALV 220

Query: 248 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 307
                + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 221 RRNGQEII----------IHVDDIAVGDIMIVKPGEKIAMDGIIVNGLSAVNQAAITGES 280

Query: 308 KPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFG 367
            P+   V + V  G  N +G I VK TK  E++T+++I+ L EEAQ  +   Q ++++F 
Sbjct: 281 VPVSKAVDDEVFAGTLNEEGLIEVKITKYVEDTTITKIIHLVEEAQGERAPAQAFVDKFA 340

Query: 368 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 427
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 341 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 400

Query: 428 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESH 487
            AI + A+KG+L+KGG  L+ + +  TVAFDKTGTLT      K +  +   ++  D+  
Sbjct: 401 SAIGNAAKKGVLVKGGVYLEKLGAIKTVAFDKTGTLT------KGVPVVTDFEVLNDQVE 460

Query: 488 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLP--SISVDSFEYFPGRGLI 547
                    EKE  ++  A+E  + HP+  A++  +   ++P  ++ V+ F    GRG+ 
Sbjct: 461 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNIPYSNVQVEEFTSITGRGIK 520

Query: 548 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQ 607
             ++G    IG       L   DF   F   EN  + +++  +T         A +   +
Sbjct: 521 GIVNGTTYYIGSPKLFKELNVSDFSLGF---ENNVKILQNQGKT---------AMIIGTE 580

Query: 608 KVTL--IHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLK 667
           K  L  I + D+ R    + I +L   G  + +MLTGD+  +A  +   VG++++   L 
Sbjct: 581 KTILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTANAIGTHVGVSDIQSELM 640

Query: 668 PEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQD 727
           P+DKL ++K++  E    + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D
Sbjct: 641 PQDKLDYIKKMQSEY-DNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGD 700

Query: 728 SISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCL 787
            +S +PF +  SR+T +++K N+T A+   ++A L  + G+L LW+ +L   G T+LV L
Sbjct: 701 DLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVAL 722

Query: 788 NSIRAL 789
           NS+R +
Sbjct: 761 NSLRLM 722

BLAST of Lsi10G002330.1 vs. ExPASy TrEMBL
Match: A0A1S3CI84 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501066 PE=3 SV=1)

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 777/823 (94.41%), Postives = 793/823 (96.35%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPI+ SE P PLLT NTNA +Q RIGPVSRFSP SSLI LNRFPIC+KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
            HFRCAAEASD R  HHHGHHH HHGCQHH SGD D VELTG QKAFVRFA+AIRWTDLA
Sbjct: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
           +S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IKDAVR STYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           ISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           S+RALN PSWSWKQDFQNLI +ARSKLNTTPETSSGTI+TATS
Sbjct: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 823

BLAST of Lsi10G002330.1 vs. ExPASy TrEMBL
Match: A0A0A0LN89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433360 PE=3 SV=1)

HSP 1 Score: 1487.2 bits (3849), Expect = 0.0e+00
Identity = 768/823 (93.32%), Postives = 786/823 (95.50%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPI+ SE P PLLT NTNA +Q R+GPVSRFSP SSLI LNRFP  +KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
            HFRCAAEAS  RH HHHGHHH HHGCQHH SGD DGVELTGAQKAFVRFA+AIRWTDLA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLDVNDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGEV+PLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
           +S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESR IKDAVR STYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           ISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLG     L VLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG-----LKVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           S+RALN PSWSWKQD QNLI DARSKLNTTPE SSGTI+TATS
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 818

BLAST of Lsi10G002330.1 vs. ExPASy TrEMBL
Match: A0A6J1L1A5 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499516 PE=3 SV=1)

HSP 1 Score: 1429.5 bits (3699), Expect = 0.0e+00
Identity = 739/823 (89.79%), Postives = 771/823 (93.68%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL +PI+ S+P   LLTR TNA A+ RIGPVS FS RSS IRL R             
Sbjct: 1   MDTLFIPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSAIRLKR------------- 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
             FRCAA+ASD RH HH  HHH HH CQHH  GDGDGVELTGAQKAFV FA+AIRWTDLA
Sbjct: 61  --FRCAADASDHRHGHH--HHHHHHSCQHHCGGDGDGVELTGAQKAFVGFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLHLCCGSAALF+TAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHL
Sbjct: 241 FALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGE+KPLQIKVG+RVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQ NKPKLQRWL
Sbjct: 301 TGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQQNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
           +SH ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISV+SFEYFPGRGL
Sbjct: 481 KSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIK+AVR S++GSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSFGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPR  V+DAIAEL+D+ KLRVMMLTGDHDSSAWK+ANAVGI+EVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGISEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           ISGVPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           SIRALN PSWSWKQDFQNLI +ARS L+TTPE SSGTIRTA+S
Sbjct: 781 SIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 806

BLAST of Lsi10G002330.1 vs. ExPASy TrEMBL
Match: A0A6J1G6Z6 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451327 PE=3 SV=1)

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 739/823 (89.79%), Postives = 769/823 (93.44%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPI+ S+P   LLTR TNA A+ RIGPVS FS RSS+IRL R             
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVIRLKR------------- 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
             FRCAA+ASD    H HGHHH HH CQHH  GDGDGVELTGAQKAFVRFA+AIRWTDLA
Sbjct: 61  --FRCAADASD----HRHGHHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLHLCCGSAALF+TAAACPYLVPKPAVK LQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHLCCGSAALFITAAACPYLVPKPAVKLLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHL
Sbjct: 241 FALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGE+KPLQIKVG+RVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMASC TVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCDTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
            SH ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISV+SFEYFPGRGL
Sbjct: 481 NSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGG LRKASLGSVDFITSFCKSENESRKIK+AVR S+YGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGPLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPR  V+DAIAEL+D+ KLRVMMLTGDHDSSAWK+ANAVGI+EVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGIDEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           ISGVPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           SIRALN PSWSWKQDFQNLI +ARS L+TTPE SSGTIRTA+S
Sbjct: 781 SIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 804

BLAST of Lsi10G002330.1 vs. ExPASy TrEMBL
Match: A0A6J1DBA2 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018894 PE=3 SV=1)

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 716/805 (88.94%), Postives = 750/805 (93.17%), Query Frame = 0

Query: 21  LTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRFRHFRCAAEASDRRHSHH 80
           + R TN  A     PV RFS RSSLI +    +  K   YPR R   CA E +     HH
Sbjct: 11  IRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAPEGA----GHH 70

Query: 81  HGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLANYLREHLHLCCGSAALF 140
           HGH   HHGCQHH   DGDGVE TGAQKAF+RFA AIRWT+LANYLREHL LCCGSAALF
Sbjct: 71  HGH---HHGCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGSAALF 130

Query: 141 VTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAAFAS 200
           +TAAACPYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTD+SGGKVNIHVLMALAAFAS
Sbjct: 131 LTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALAAFAS 190

Query: 201 IFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSD 260
           IFMGNPLEGGLLLVMFN+AHIAEEYFTKQSMIDVKELKESHPDFALVLD+NDDNLPDTSD
Sbjct: 191 IFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLPDTSD 250

Query: 261 LEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPG 320
           LEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPL+IKVGERVPG
Sbjct: 251 LEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGERVPG 310

Query: 321 GARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVA 380
           GARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL+EFGENYSKVVVVLSVA
Sbjct: 311 GARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVA 370

Query: 381 VALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLK 440
           VALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLK
Sbjct: 371 VALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLK 430

Query: 441 GGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEAL 500
           GGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+++H ASCCIPSCEKEAL
Sbjct: 431 GGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCEKEAL 490

Query: 501 AVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKLRK 560
           AVAAAMEKGTTHPIGRAVVDHS GKDLPSISV+SFEYFPGRGLIATLHGIKSGIGGKLRK
Sbjct: 491 AVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGGKLRK 550

Query: 561 ASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVV 620
           ASLGSVDFITSFCKSENESRKIK+AVR S+YGSEFVHAALSVDQKVTLIHLEDQPRP ++
Sbjct: 551 ASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPRPGML 610

Query: 621 DAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGG 680
           +AI+EL+D  K+RVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGG
Sbjct: 611 NAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGG 670

Query: 681 LIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSL 740
           LIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+IS VPFCIAKSRQTTSL
Sbjct: 671 LIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQTTSL 730

Query: 741 VKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQ 800
           VKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQ
Sbjct: 731 VKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQ 790

Query: 801 NLICDARSKLNTTPETSSGTIRTAT 826
           NL+ DARS++  T +TSSGTI+TA+
Sbjct: 791 NLVRDARSRVLATRQTSSGTIQTAS 806

BLAST of Lsi10G002330.1 vs. NCBI nr
Match: XP_008462778.1 (PREDICTED: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis melo])

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 777/823 (94.41%), Postives = 793/823 (96.35%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPI+ SE P PLLT NTNA +Q RIGPVSRFSP SSLI LNRFPIC+KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
            HFRCAAEASD R  HHHGHHH HHGCQHH SGD D VELTG QKAFVRFA+AIRWTDLA
Sbjct: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
           +S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IKDAVR STYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           ISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           S+RALN PSWSWKQDFQNLI +ARSKLNTTPETSSGTI+TATS
Sbjct: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 823

BLAST of Lsi10G002330.1 vs. NCBI nr
Match: XP_038901378.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1510.7 bits (3910), Expect = 0.0e+00
Identity = 780/823 (94.78%), Postives = 795/823 (96.60%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPIS SE P PLLTRNTNA AQ RIGP+SRFSPRSSLIRLNRFPI  KK+SYPRF
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
            HFRCAAEASD    H HGHHH HHGCQHH  GD DGVELTGAQKAFVRFA+AIRWTDLA
Sbjct: 61  GHFRCAAEASD----HRHGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GG+VNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGRVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
           +SHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+SFEYFPGRGL
Sbjct: 481 KSHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVR STYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRASTYGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPRP VVDAIAEL+D+GKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLARRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           IS VPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISAVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           S+RALNQPSWSWKQDFQNL+ + RSKLN  PETSSGTI+TATS
Sbjct: 781 SVRALNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTATS 819

BLAST of Lsi10G002330.1 vs. NCBI nr
Match: XP_004151907.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis sativus] >KAE8652344.1 hypothetical protein Csa_021970 [Cucumis sativus])

HSP 1 Score: 1506.9 bits (3900), Expect = 0.0e+00
Identity = 774/823 (94.05%), Postives = 792/823 (96.23%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPI+ SE P PLLT NTNA +Q R+GPVSRFSP SSLI LNRFP  +KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
            HFRCAAEAS  RH HHHGHHH HHGCQHH SGD DGVELTGAQKAFVRFA+AIRWTDLA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLDVNDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGEV+PLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
           +S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESR IKDAVR STYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           ISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           S+RALN PSWSWKQD QNLI DARSKLNTTPE SSGTI+TATS
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 823

BLAST of Lsi10G002330.1 vs. NCBI nr
Match: XP_038901377.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1501.1 bits (3885), Expect = 0.0e+00
Identity = 780/837 (93.19%), Postives = 795/837 (94.98%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPIS SE P PLLTRNTNA AQ RIGP+SRFSPRSSLIRLNRFPI  KK+SYPRF
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
            HFRCAAEASD    H HGHHH HHGCQHH  GD DGVELTGAQKAFVRFA+AIRWTDLA
Sbjct: 61  GHFRCAAEASD----HRHGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GG+VNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGRVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
           +SHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+SFEYFPGRGL
Sbjct: 481 KSHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFPGRGL 540

Query: 544 IATLHGIK--------------SGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTS 603
           IATLHGIK              SGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVR S
Sbjct: 541 IATLHGIKVEGEEYLHSISILQSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRAS 600

Query: 604 TYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVA 663
           TYGSEFVHAALSVDQKVTLIHLEDQPRP VVDAIAEL+D+GKLRVMMLTGDHDSSAWKVA
Sbjct: 601 TYGSEFVHAALSVDQKVTLIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSAWKVA 660

Query: 664 NAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASA 723
           NAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLA RASA
Sbjct: 661 NAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLARRASA 720

Query: 724 TATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLT 783
           TATAVADVLLLQDSIS VPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLT
Sbjct: 721 TATAVADVLLLQDSISAVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLT 780

Query: 784 VLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           VLLHEGGTLLVCLNS+RALNQPSWSWKQDFQNL+ + RSKLN  PETSSGTI+TATS
Sbjct: 781 VLLHEGGTLLVCLNSVRALNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTATS 833

BLAST of Lsi10G002330.1 vs. NCBI nr
Match: XP_023533453.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1433.3 bits (3709), Expect = 0.0e+00
Identity = 742/823 (90.16%), Postives = 770/823 (93.56%), Query Frame = 0

Query: 4   MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 63
           MDTL VPI+ S+P   LLTR TNA A+ RIGPVS FS RSS+IRL R             
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVIRLKR------------- 60

Query: 64  RHFRCAAEASDRRHSHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLA 123
             FRCAA+ASD RH H   HHH HH CQHH  GDGDGVELTGAQKAFVRFA AIRWTDLA
Sbjct: 61  --FRCAADASDHRHGH---HHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAKAIRWTDLA 120

Query: 124 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLS 183
           NYLREHLHLCCGSAALF+TAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTD+S
Sbjct: 121 NYLREHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 184 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 243
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPD 240

Query: 244 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 303
           FALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHL
Sbjct: 241 FALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHL 300

Query: 304 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWL 363
           TGE+KPLQIKVG+RVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 364 NEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 423
           +EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPL 420

Query: 424 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 483
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 484 ESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGL 543
            SH ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISV+SFEYFPGRGL
Sbjct: 481 NSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGL 540

Query: 544 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVD 603
           IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIK+AVR S+YGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVD 600

Query: 604 QKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 663
           QKVTLIHLEDQPR  V+DAIAEL+D+  LRVMMLTGDHDSSAWK+ANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRAGVLDAIAELQDQANLRVMMLTGDHDSSAWKIANAVGINEVYYSLKP 660

Query: 664 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 723
           EDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDS 720

Query: 724 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 783
           ISGVPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLN 780

Query: 784 SIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 827
           SIRALN PSWSWKQDFQNLI +ARS L+TTPE SSGTIRTA+S
Sbjct: 781 SIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 805

BLAST of Lsi10G002330.1 vs. TAIR 10
Match: AT4G37270.1 (heavy metal atpase 1 )

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 571/794 (71.91%), Postives = 649/794 (81.74%), Query Frame = 0

Query: 32  RIGPVSRFS--PRSSLIRLNRFPICYKKLSYPRFRHFRCAAEASDRRHSHHHGHHHGHHG 91
           R+  V+ FS  P  +L+R     I       PR    R      D  H HHH     HH 
Sbjct: 25  RLARVNSFSILPPKTLLRQKPLRISASLNLPPRSIRLRA---VEDHHHDHHHDDEQDHHN 84

Query: 92  CQHHSSGDG-DGVEL---TGAQKAFVRFADAIRWTDLANYLREHLHLCCGSAALFVTAAA 151
             HH    G   VEL   +  QK    FA AI W  LANYLREHLHLCC +AA+F+ AA 
Sbjct: 85  HHHHHHQHGCCSVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAAAMFLAAAV 144

Query: 152 CPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAAFASIFMGN 211
           CPYL P+P +K LQN F+ V FPLVGVSASLDAL D++GGKVNIHVLMALAAFAS+FMGN
Sbjct: 145 CPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAAFASVFMGN 204

Query: 212 PLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRR 271
            LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL+++V++ N+P+ SDL ++ 
Sbjct: 205 ALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPNISDLSYKS 264

Query: 272 VPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNL 331
           VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVKPL+ K G+RVPGGARNL
Sbjct: 265 VPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDRVPGGARNL 324

Query: 332 DGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIG 391
           DGR+IVKATKAW +STL++IVQLTEEA  NKPKLQRWL+EFGENYSKVVVVLS+A+A +G
Sbjct: 325 DGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVLSLAIAFLG 384

Query: 392 PLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGILLKGGHVL 451
           P LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGILLKG  VL
Sbjct: 385 PFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGILLKGAQVL 444

Query: 452 DAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAA 511
           DA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   +CCIP+CEKEALAVAAA
Sbjct: 445 DALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEKEALAVAAA 504

Query: 512 MEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKS-GIGGKLRKASLG 571
           MEKGTTHPIGRAVVDHSVGKDLPSI V+SFEYFPGRGL AT++G+K+     +LRKASLG
Sbjct: 505 MEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEESRLRKASLG 564

Query: 572 SVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIA 631
           S++FITS  KSE+ES++IKDAV  S+YG +FVHAALSVDQKVTLIHLEDQPRP V   IA
Sbjct: 565 SIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPRPGVSGVIA 624

Query: 632 ELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMV 691
           EL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE GGGLIMV
Sbjct: 625 ELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIAREAGGGLIMV 684

Query: 692 GEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQN 751
           GEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GVPFC+AKSRQTTSLVKQN
Sbjct: 685 GEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQTTSLVKQN 744

Query: 752 VTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNLIC 811
           V LAL+SI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNS+R LN PSWSWKQD  +LI 
Sbjct: 745 VALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWKQDIVHLIN 804

Query: 812 DARSKLNTTPETSS 819
             RS+  T+  ++S
Sbjct: 805 KLRSQEPTSSSSNS 814

BLAST of Lsi10G002330.1 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 205.7 bits (522), Expect = 1.4e-52
Identity = 180/660 (27.27%), Postives = 316/660 (47.88%), Query Frame = 0

Query: 162 FIAVAFPLVGVSASL-DALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 221
           ++AVA    G+   L  A   +   +++I++L+ +   A++ M + +E   ++ +F ++ 
Sbjct: 122 WLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISD 181

Query: 222 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 281
             E   + ++   ++ L    P  A++ +  ++            V V +V+V + + V 
Sbjct: 182 WLETRASYKATSVMQSLMSLAPQKAIIAETGEE------------VEVDEVKVDTVVAVK 241

Query: 282 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEES 341
           AGE++P+D  V  G   +  + LTGE  P+  +    V  G  NL+G I VK T    + 
Sbjct: 242 AGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDC 301

Query: 342 TLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 401
            ++++ +L EEAQ +K K QR +++  + Y+  ++++S  VA++ P++ K         +
Sbjct: 302 VVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIV-PVIMK-----VHNLK 361

Query: 402 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 461
              + AL ++V+  PC L  + P+A   A++  A  G+L+K    LD ++    VAFDKT
Sbjct: 362 HWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKT 421

Query: 462 GTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 521
           GT+T G  +                  F S       +  L   +++E  ++HP+   +V
Sbjct: 422 GTITRGEFIV---------------IDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIV 481

Query: 522 DH--SVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSEN 581
           D+  SV  +     V+ ++ FPG G+   + G    IG K   +  G        C +  
Sbjct: 482 DYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAG--------CSTVP 541

Query: 582 ESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMML 641
           E       +   T G + V      ++     +L D  R  V  A+AEL+  G ++  ML
Sbjct: 542 E-------IEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLG-IKTAML 601

Query: 642 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 701
           TGD+ ++A      +G  ++ V+  L PEDK   ++E  +E  G   MVG+G+NDAPALA
Sbjct: 602 TGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE--GPTAMVGDGVNDAPALA 661

Query: 702 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLA 761
            A +GI +    SA AT   +++L+ + I  +P  +  +R+    V +NV   LS IL A
Sbjct: 662 TADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENV--CLSIILKA 721

Query: 762 SLPSV--LGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNLIC--DARSKLN 812
            + ++   G   +W  VL+  G  LLV  NS+  L +     K+   N  C   + SKLN
Sbjct: 722 GILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLRE-----KKKIGNKKCYRASTSKLN 723

BLAST of Lsi10G002330.1 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 204.5 bits (519), Expect = 3.2e-52
Identity = 172/634 (27.13%), Postives = 307/634 (48.42%), Query Frame = 0

Query: 162 FIAVAFPLVGVSASL-DALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 221
           ++AVA  + G+   L  A+  L+  +++I++L+ +   A+I M +  E  +++ +F +A 
Sbjct: 112 WLAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAE 171

Query: 222 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 281
             +   + ++   ++ L    P  A++ +  ++            V V +++  + I V 
Sbjct: 172 WLQSRASYKASAVMQSLMSLAPQKAVIAETGEE------------VEVDELKTNTVIAVK 231

Query: 282 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEES 341
           AGE++P+D  V  G   +  + LTGE  P+       V  G  NL+G I V  T   E+ 
Sbjct: 232 AGETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDC 291

Query: 342 TLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 401
            ++++ +L EEAQ +K + QR++++  + Y+  ++++S+    I P   K         +
Sbjct: 292 VVAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAI-PFALK-----VHNLK 351

Query: 402 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 461
             V+ AL ++V+A PC L  + P+A   A++  A  G+L+KG   L+ +A    VAFDKT
Sbjct: 352 HWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKT 411

Query: 462 GTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 521
           GT+T G  +                  F S       +  L   ++ E  ++HP+  AVV
Sbjct: 412 GTITRGEFIV---------------MDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVV 471

Query: 522 DH--SVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSEN 581
           D+  SV  +    +V+ ++ FPG G+   + G +  IG K   +  G        C S  
Sbjct: 472 DYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRAG--------CLSVP 531

Query: 582 ESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMML 641
           +       +   T G + +      +    + +L D  R  V  A+ EL+  G +++ ML
Sbjct: 532 D-------IDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLG-IKIAML 591

Query: 642 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 701
           TGD+ ++A      +G  ++ V   L PEDK   +K++ RE  G   MVG+G+NDAPALA
Sbjct: 592 TGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKRE-EGPTAMVGDGLNDAPALA 651

Query: 702 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLA 761
            A +GI +    SA AT   +++L+ + I  +P  I  +++    V +NV ++++     
Sbjct: 652 TADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAI 695

Query: 762 SLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALN 790
              +  G   +W  VL   G  LLV LNS+  L+
Sbjct: 712 LALAFAGHPLIWAAVLADVGTCLLVILNSMLLLS 695

BLAST of Lsi10G002330.1 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 193.7 bits (491), Expect = 5.7e-49
Identity = 200/700 (28.57%), Postives = 319/700 (45.57%), Query Frame = 0

Query: 116 AIRWTDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFI---AVAFPLVGV 175
           A  WT +A        LCCGS    +  +   ++        L N ++        L+G 
Sbjct: 180 AFAWTLVA--------LCCGSHTSHILHSLGIHIAHGGIWDLLHNSYVKGGLAVGALLGP 239

Query: 176 SASL--DALTDLSGGKVNIHVLMALAAFAS-----IFMGNPL---------EGGLLLVMF 235
              L  D +        N++ L+ L + A+     I + NP          E  +LL   
Sbjct: 240 GRELLFDGIKAFGKRSPNMNSLVGLGSMAAFSISLISLVNPELEWDASFFDEPVMLLGFV 299

Query: 236 NMAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDVNDDNLPDTSDLEHR----RVPVRDV 295
            +    EE    Q+  D+ EL         LV+  +D+N P  S L        V V D+
Sbjct: 300 LLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICINVSVDDI 359

Query: 296 EVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIV 355
            VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N DG + +
Sbjct: 360 RVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINWDGPLRI 419

Query: 356 KATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAV----ALIGPL 415
           KA+     ST+S+IV++ E+AQ N   +QR  +     +   ++ LS         +G  
Sbjct: 420 KASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFWYYVGSH 479

Query: 416 LFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSCARKGIL 475
           +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  A++G L
Sbjct: 480 IF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLGAKRGYL 539

Query: 476 LKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKE 535
           ++GG VL+ +AS   VA DKTGTLT G  V   +  + G++                E+E
Sbjct: 540 IRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE----------------EQE 599

Query: 536 ALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKL 595
            L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G         
Sbjct: 600 VLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--------- 659

Query: 596 RKASLGSVDFITSFCKSENES-----------RKIKDAVRTSTYGSEFVHAALSVDQKVT 655
           R  ++GS+++++     +N+S            K+ +   TS Y    V+     +  + 
Sbjct: 660 RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGREGEGIIG 719

Query: 656 LIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGI--NEVYYSLKPED 715
            I + D  R +    +A L++KG ++ ++L+GD + +   VA  VGI      YSL PE 
Sbjct: 720 AIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNYSLSPEK 779

Query: 716 KLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATA-VADVLLLQDSI 765
           K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+L+++ +
Sbjct: 780 KFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVILVRNKL 839

BLAST of Lsi10G002330.1 vs. TAIR 10
Match: AT5G21930.2 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 193.7 bits (491), Expect = 5.7e-49
Identity = 200/700 (28.57%), Postives = 319/700 (45.57%), Query Frame = 0

Query: 116 AIRWTDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFI---AVAFPLVGV 175
           A  WT +A        LCCGS    +  +   ++        L N ++        L+G 
Sbjct: 180 AFAWTLVA--------LCCGSHTSHILHSLGIHIAHGGIWDLLHNSYVKGGLAVGALLGP 239

Query: 176 SASL--DALTDLSGGKVNIHVLMALAAFAS-----IFMGNPL---------EGGLLLVMF 235
              L  D +        N++ L+ L + A+     I + NP          E  +LL   
Sbjct: 240 GRELLFDGIKAFGKRSPNMNSLVGLGSMAAFSISLISLVNPELEWDASFFDEPVMLLGFV 299

Query: 236 NMAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDVNDDNLPDTSDLEHR----RVPVRDV 295
            +    EE    Q+  D+ EL         LV+  +D+N P  S L        V V D+
Sbjct: 300 LLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICINVSVDDI 359

Query: 296 EVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIV 355
            VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N DG + +
Sbjct: 360 RVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINWDGPLRI 419

Query: 356 KATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAV----ALIGPL 415
           KA+     ST+S+IV++ E+AQ N   +QR  +     +   ++ LS         +G  
Sbjct: 420 KASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFWYYVGSH 479

Query: 416 LFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSCARKGIL 475
           +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  A++G L
Sbjct: 480 IF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLGAKRGYL 539

Query: 476 LKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKE 535
           ++GG VL+ +AS   VA DKTGTLT G  V   +  + G++                E+E
Sbjct: 540 IRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE----------------EQE 599

Query: 536 ALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKL 595
            L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G         
Sbjct: 600 VLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--------- 659

Query: 596 RKASLGSVDFITSFCKSENES-----------RKIKDAVRTSTYGSEFVHAALSVDQKVT 655
           R  ++GS+++++     +N+S            K+ +   TS Y    V+     +  + 
Sbjct: 660 RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGREGEGIIG 719

Query: 656 LIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGI--NEVYYSLKPED 715
            I + D  R +    +A L++KG ++ ++L+GD + +   VA  VGI      YSL PE 
Sbjct: 720 AIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNYSLSPEK 779

Query: 716 KLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATA-VADVLLLQDSI 765
           K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+L+++ +
Sbjct: 780 KFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVILVRNKL 839

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3H50.0e+0071.91Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis tha... [more]
Q600483.2e-7329.03Probable cadmium-transporting ATPase OS=Listeria monocytogenes OX=1639 GN=cadA P... [more]
P584144.2e-7329.11Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (str... [more]
Q6GIX15.2e-7129.26Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) O... [more]
P200211.5e-7029.54Cadmium-transporting ATPase OS=Staphylococcus aureus OX=1280 GN=cadA PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CI840.0e+0094.41probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucumis melo OX... [more]
A0A0A0LN890.0e+0093.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433360 PE=3 SV=1[more]
A0A6J1L1A50.0e+0089.79probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxim... [more]
A0A6J1G6Z60.0e+0089.79probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita mosch... [more]
A0A6J1DBA20.0e+0088.94probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Momordica chara... [more]
Match NameE-valueIdentityDescription
XP_008462778.10.0e+0094.41PREDICTED: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumi... [more]
XP_038901378.10.0e+0094.78probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X2 [Beninc... [more]
XP_004151907.10.0e+0094.05probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis sativus] ... [more]
XP_038901377.10.0e+0093.19probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Beninc... [more]
XP_023533453.10.0e+0090.16probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
AT4G37270.10.0e+0071.91heavy metal atpase 1 [more]
AT2G19110.11.4e-5227.27heavy metal atpase 4 [more]
AT4G30110.13.2e-5227.13heavy metal atpase 2 [more]
AT5G21930.15.7e-4928.57P-type ATPase of Arabidopsis 2 [more]
AT5G21930.25.7e-4928.57P-type ATPase of Arabidopsis 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00119CATATPASEcoord: 607..618
score: 36.75
coord: 630..640
score: 48.97
coord: 455..469
score: 52.32
coord: 684..703
score: 53.23
coord: 708..720
score: 30.38
NoneNo IPR availableGENE3D2.70.150.10coord: 228..353
e-value: 2.1E-21
score: 78.1
NoneNo IPR availablePFAMPF00122E1-E2_ATPasecoord: 262..435
e-value: 1.7E-43
score: 148.2
NoneNo IPR availablePFAMPF00702Hydrolasecoord: 453..697
e-value: 3.2E-31
score: 109.3
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 437..737
e-value: 2.102E-44
score: 146.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..98
NoneNo IPR availablePANTHERPTHR43079PROBABLE CADMIUM/ZINC-TRANSPORTING ATPASE HMA1coord: 63..812
IPR027256P-type ATPase, subfamily IBTIGRFAMTIGR01525TIGR01525coord: 189..786
e-value: 2.6E-167
score: 556.0
IPR001757P-type ATPaseTIGRFAMTIGR01494TIGR01494coord: 264..476
e-value: 5.4E-29
score: 98.9
coord: 588..764
e-value: 8.8E-35
score: 118.0
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 448..735
e-value: 9.2E-58
score: 197.9
IPR023299P-type ATPase, cytoplasmic domain NGENE3D3.40.1110.10coord: 466..615
e-value: 9.2E-58
score: 197.9
IPR044492P-type ATPase, haloacid dehalogenase domainSFLDSFLDF00027p-type_atpasecoord: 437..737
e-value: 2.102E-44
score: 146.6
IPR018303P-type ATPase, phosphorylation sitePROSITEPS00154ATPASE_E1_E2coord: 457..463
IPR023298P-type ATPase, transmembrane domain superfamilySUPERFAMILY81665Calcium ATPase, transmembrane domain Mcoord: 191..767
IPR008250P-type ATPase, A domain superfamilySUPERFAMILY81653Calcium ATPase, transduction domain Acoord: 263..348
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 453..784

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi10G002330Lsi10G002330gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi10G002330.1.exon.1Lsi10G002330.1.exon.1exon
Lsi10G002330.1.exon.2Lsi10G002330.1.exon.2exon
Lsi10G002330.1.exon.3Lsi10G002330.1.exon.3exon
Lsi10G002330.1.exon.4Lsi10G002330.1.exon.4exon
Lsi10G002330.1.exon.5Lsi10G002330.1.exon.5exon
Lsi10G002330.1.exon.6Lsi10G002330.1.exon.6exon
Lsi10G002330.1.exon.7Lsi10G002330.1.exon.7exon
Lsi10G002330.1.exon.8Lsi10G002330.1.exon.8exon
Lsi10G002330.1.exon.9Lsi10G002330.1.exon.9exon
Lsi10G002330.1.exon.10Lsi10G002330.1.exon.10exon
Lsi10G002330.1.exon.11Lsi10G002330.1.exon.11exon
Lsi10G002330.1.exon.12Lsi10G002330.1.exon.12exon
Lsi10G002330.1.exon.13Lsi10G002330.1.exon.13exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi10G002330.1.CDS.13Lsi10G002330.1.CDS.13CDS
Lsi10G002330.1.CDS.12Lsi10G002330.1.CDS.12CDS
Lsi10G002330.1.CDS.11Lsi10G002330.1.CDS.11CDS
Lsi10G002330.1.CDS.10Lsi10G002330.1.CDS.10CDS
Lsi10G002330.1.CDS.9Lsi10G002330.1.CDS.9CDS
Lsi10G002330.1.CDS.8Lsi10G002330.1.CDS.8CDS
Lsi10G002330.1.CDS.7Lsi10G002330.1.CDS.7CDS
Lsi10G002330.1.CDS.6Lsi10G002330.1.CDS.6CDS
Lsi10G002330.1.CDS.5Lsi10G002330.1.CDS.5CDS
Lsi10G002330.1.CDS.4Lsi10G002330.1.CDS.4CDS
Lsi10G002330.1.CDS.3Lsi10G002330.1.CDS.3CDS
Lsi10G002330.1.CDS.2Lsi10G002330.1.CDS.2CDS
Lsi10G002330.1.CDS.1Lsi10G002330.1.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi10G002330.1.five_prime_UTR.1Lsi10G002330.1.five_prime_UTR.1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi10G002330.1Lsi10G002330.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006812 cation transport
biological_process GO:0098655 cation transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0019829 ATPase-coupled cation transmembrane transporter activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0140603 obsolete ATP hydrolysis activity
molecular_function GO:0005215 transporter activity
molecular_function GO:0046872 metal ion binding