Lsi08G005860.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G005860.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	MRN complex-interacting protein isoform X2
Location	chr08: 14132776 .. 14134121 (+)
Sequence length	1096
RNA-Seq Expression	Lsi08G005860.1
Synteny	Lsi08G005860.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAATCAAAATCAAAATCCGTAAAAGCATTTGAGAATCGAAGTAGGAAATGGAGACGATCTTCATCGCCGTCCAGTGTTTTCAATGCTTCACCATGCAGGTACCGATCTCTCTCTCTTTGTTTCTTTGTCCTTCAATGTCTCTCTCTGTAACTTCTGTAATGGCGGTGGTTTCGATTTAGGTGAAGCAGCAGCGAAAGAGCGGGAATAAGTGGATCTGTGCAGTGTGTAATGAGAAGCAATCGGTGCAGAAGGTGTTTGCTCGAGCTCCTATGGCTAAAGACGTTCGTAAGTTCGTACAGAGCTTCAATATGTCTCGGAAATATGCGGATGAACAGGCTAAAGTCGACGAGCTACTTGATGGAGGAATTATTGACGACGATAAACTCGATTGCAGTGTGAGGAGAAAAAGACGTAACGACTGGAATGAATATCTCGATCCCGAGGAACATCGCAGCCAAAGCGGAGGAGAAAACGAACGAGAATTAGGTCACCGAATTTTTGAATACTTATACTTACAATTACTTTTTTCAACTTCGAAGAAAAATCGTTGTTAGATAATGATTTTTTTCCTGGATCAGAGAGTGATTTTACTGACGGAAAGATTGTGACGGAGCTTCCAGAAGTTAAAACCAGGAAATCTGGTTCAACGAGCTGTTCAAGCTGGTCGGAAGGTGGAGAAGGCGATGGATATTACAGACCAGTTTTCTCGAAGAGGAAACATATTGCCAAGAATTTGAGCTCTCAAGGTAAAATTTGATGTTCAAATAACTTCTTGCACTTTTTCATGACAAAAATTTGAGCAAAAAATGCCACAAAACTGCAGATGAGATAAAAAACTGGGAGTCGCCATCAGCCATTCACGCATTGAAATGGAGTAAAGACGACGATGAACAAATTTTCTCGAAGGTGAAGAACTTGAGTGCACCTTTCCAGGACAAAAAGTTGAGGAAAAACAGTCATAAAATTGGAGATGAGATTCAAAACTGGGAGTTGCCATCAGACATTGAAGAATCAAATTGGAGTAAAGGTATATCAAAAGATGATGAAAGTGCAAGGACGAAGACTTGCATATTAGCTAAGGTAAGAGGACCTTCCAAGTGGAGTGAATACATAACTGAAGAAAACTGCGGATTCAGAAATGGAGAGAATATGGCAACAGAACAGGGCGTAGATAACTTGAAGATGATGATAAACGATGAAATTGTTGAAGACGATATCCACCCTGATTTCAAGTGATATTGACTCTGCTAATGAGATTTTGTTTAGCAACTATTGTTTTAGTAGATTTTGAGTTAATTCAAACGCTTGGAAATATATTTTTGGGTAAAATAACAATTTTTGATC

mRNA sequence

CAAAATCAAAATCAAAATCCGTAAAAGCATTTGAGAATCGAAGTAGGAAATGGAGACGATCTTCATCGCCGTCCAGTGTTTTCAATGCTTCACCATGCAGGTGAAGCAGCAGCGAAAGAGCGGGAATAAGTGGATCTGTGCAGTGTGTAATGAGAAGCAATCGGTGCAGAAGGTGTTTGCTCGAGCTCCTATGGCTAAAGACGTTCGTAAGTTCGTACAGAGCTTCAATATGTCTCGGAAATATGCGGATGAACAGGCTAAAGTCGACGAGCTACTTGATGGAGGAATTATTGACGACGATAAACTCGATTGCAGTGTGAGGAGAAAAAGACGTAACGACTGGAATGAATATCTCGATCCCGAGGAACATCGCAGCCAAAGCGGAGGAGAAAACGAACGAGAATTAGAGAGTGATTTTACTGACGGAAAGATTGTGACGGAGCTTCCAGAAGTTAAAACCAGGAAATCTGGTTCAACGAGCTGTTCAAGCTGGTCGGAAGGTGGAGAAGGCGATGGATATTACAGACCAGTTTTCTCGAAGAGGAAACATATTGCCAAGAATTTGAGCTCTCAAGATGAGATAAAAAACTGGGAGTCGCCATCAGCCATTCACGCATTGAAATGGAGTAAAGACGACGATGAACAAATTTTCTCGAAGGTGAAGAACTTGAGTGCACCTTTCCAGGACAAAAAGTTGAGGAAAAACAGTCATAAAATTGGAGATGAGATTCAAAACTGGGAGTTGCCATCAGACATTGAAGAATCAAATTGGAGTAAAGGTATATCAAAAGATGATGAAAGTGCAAGGACGAAGACTTGCATATTAGCTAAGGTAAGAGGACCTTCCAAGTGGAGTGAATACATAACTGAAGAAAACTGCGGATTCAGAAATGGAGAGAATATGGCAACAGAACAGGGCGTAGATAACTTGAAGATGATGATAAACGATGAAATTGTTGAAGACGATATCCACCCTGATTTCAAGTGATATTGACTCTGCTAATGAGATTTTGTTTAGCAACTATTGTTTTAGTAGATTTTGAGTTAATTCAAACGCTTGGAAATATATTTTTGGGTAAAATAACAATTTTTGATC

Coding sequence (CDS)

ATGGAGACGATCTTCATCGCCGTCCAGTGTTTTCAATGCTTCACCATGCAGGTGAAGCAGCAGCGAAAGAGCGGGAATAAGTGGATCTGTGCAGTGTGTAATGAGAAGCAATCGGTGCAGAAGGTGTTTGCTCGAGCTCCTATGGCTAAAGACGTTCGTAAGTTCGTACAGAGCTTCAATATGTCTCGGAAATATGCGGATGAACAGGCTAAAGTCGACGAGCTACTTGATGGAGGAATTATTGACGACGATAAACTCGATTGCAGTGTGAGGAGAAAAAGACGTAACGACTGGAATGAATATCTCGATCCCGAGGAACATCGCAGCCAAAGCGGAGGAGAAAACGAACGAGAATTAGAGAGTGATTTTACTGACGGAAAGATTGTGACGGAGCTTCCAGAAGTTAAAACCAGGAAATCTGGTTCAACGAGCTGTTCAAGCTGGTCGGAAGGTGGAGAAGGCGATGGATATTACAGACCAGTTTTCTCGAAGAGGAAACATATTGCCAAGAATTTGAGCTCTCAAGATGAGATAAAAAACTGGGAGTCGCCATCAGCCATTCACGCATTGAAATGGAGTAAAGACGACGATGAACAAATTTTCTCGAAGGTGAAGAACTTGAGTGCACCTTTCCAGGACAAAAAGTTGAGGAAAAACAGTCATAAAATTGGAGATGAGATTCAAAACTGGGAGTTGCCATCAGACATTGAAGAATCAAATTGGAGTAAAGGTATATCAAAAGATGATGAAAGTGCAAGGACGAAGACTTGCATATTAGCTAAGGTAAGAGGACCTTCCAAGTGGAGTGAATACATAACTGAAGAAAACTGCGGATTCAGAAATGGAGAGAATATGGCAACAGAACAGGGCGTAGATAACTTGAAGATGATGATAAACGATGAAATTGTTGAAGACGATATCCACCCTGATTTCAAGTGA

Protein sequence

METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFNMSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELESDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKNWESPSAIHALKWSKDDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIEESNWSKGISKDDESARTKTCILAKVRGPSKWSEYITEENCGFRNGENMATEQGVDNLKMMINDEIVEDDIHPDFK

Homology

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BLAST of Lsi08G005860.1 vs. ExPASy Swiss-Prot
Match: Q32PP1 (MRN complex-interacting protein OS=Danio rerio OX=7955 GN=mrnip PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.2e-06
Identity = 59/208 (28.37%), Postives = 83/208 (39.90%), Query Frame = 0


Query: 5   FIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFNMSRK 64
           F  ++CF C T QV QQ K   KW C VC EKQS+ K F R   A D R+ VQ  N  R 
Sbjct: 5   FNVLRCFSCQTFQV-QQVKKAKKWTCKVCGEKQSLIKEFGRG-AAADCRRHVQKLNALRG 64

Query: 65  YADEQAKVDELLDGGIIDDDKLD--------CSVRRKRRNDWNEYLDPEEHRSQSGGENE 124
               Q    +LL  G  +++  D                + W++Y D       + G NE
Sbjct: 65  -EQHQLNTQQLLAQGDEENENEDVYEVLDPKSEQEEAHVSRWSKYTD-----QTTEGPNE 124

Query: 125 RELESDFTDGKIVTELPEVK---TRKSGSTS--------CSSWSEGGEGDGYYRP----- 184
            + + D  D  + TE P+ +   TRK    S        C ++ E   G   +R      
Sbjct: 125 EKDDED-EDENVYTERPQFRIQGTRKRKKMSSLEPFGGNCDNYEESRAGYSQFRKQAYLP 184

Query: 185 -VFSKRKHIAKNLSSQDEIKNWESPSAI 188
               KR   + N  S  +  N + P  +
Sbjct: 185 LQLEKRSSSSWNKGSVSKYSNCQRPDDV 203

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BLAST of Lsi08G005860.1 vs. ExPASy TrEMBL
Match: A0A5D3BXL1 (UPF0544 protein C5orf45-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G00830 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.0e-132
Identity = 253/314 (80.57%), Postives = 270/314 (85.99%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MATIFIAVQCFQCSTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYA+EQA VD+L+ GGI+DDDKLDCS RRKRRNDW+EYLDPEE RS SG ENE ELE
Sbjct: 61  MSRKYANEQANVDDLIHGGIVDDDKLDCSGRRKRRNDWSEYLDPEEDRSHSGRENEGELE 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SD    KI TELP++KT KSGS SCSSWSE GEGDG YRP+FSKRKHIAKNLSSQDEI+N
Sbjct: 121 SDL---KIATELPDIKTWKSGSRSCSSWSE-GEGDGLYRPLFSKRKHIAKNLSSQDEIQN 180

Query: 181 WESPSAIHALKWSKDDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIEESN 240
           WESPSAIH  K SK DDEQ FS  K LSAPFQDKK+ KNSHK+  EIQNWELPS+IE S 
Sbjct: 181 WESPSAIHTSKLSKGDDEQTFSTAKYLSAPFQDKKMSKNSHKVESEIQNWELPSEIEASK 240

Query: 241 WSKGISKDDESARTKTCILAKVRG-PSKWSEYIT-EENCGFRNGENMATEQGVDNLKMMI 300
           W    +KDDE++RTK+CIL KVRG  SKWSEYIT EENCG RN ENMA EQG+D LK MI
Sbjct: 241 W----TKDDENSRTKSCILGKVRGASSKWSEYITEEENCGGRNEENMAREQGMDILK-MI 300

Query: 301 NDEIVEDDIHPDFK 313
           NDEIVEDDIHPDFK
Sbjct: 301 NDEIVEDDIHPDFK 305

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BLAST of Lsi08G005860.1 vs. ExPASy TrEMBL
Match: A0A1S3C3H7 (UPF0544 protein C5orf45 homolog OS=Cucumis melo OX=3656 GN=LOC103496578 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.0e-132
Identity = 253/314 (80.57%), Postives = 270/314 (85.99%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MATIFIAVQCFQCSTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYA+EQA VD+L+ GGI+DDDKLDCS RRKRRNDW+EYLDPEE RS SG ENE ELE
Sbjct: 61  MSRKYANEQANVDDLIHGGIVDDDKLDCSGRRKRRNDWSEYLDPEEDRSHSGRENEGELE 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SD    KI TELP++KT KSGS SCSSWSE GEGDG YRP+FSKRKHIAKNLSSQDEI+N
Sbjct: 121 SDL---KIATELPDIKTWKSGSRSCSSWSE-GEGDGLYRPLFSKRKHIAKNLSSQDEIQN 180

Query: 181 WESPSAIHALKWSKDDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIEESN 240
           WESPSAIH  K SK DDEQ FS  K LSAPFQDKK+ KNSHK+  EIQNWELPS+IE S 
Sbjct: 181 WESPSAIHTSKLSKGDDEQTFSTAKYLSAPFQDKKMSKNSHKVESEIQNWELPSEIEASK 240

Query: 241 WSKGISKDDESARTKTCILAKVRG-PSKWSEYIT-EENCGFRNGENMATEQGVDNLKMMI 300
           W    +KDDE++RTK+CIL KVRG  SKWSEYIT EENCG RN ENMA EQG+D LK MI
Sbjct: 241 W----TKDDENSRTKSCILGKVRGASSKWSEYITEEENCGGRNEENMAREQGMDILK-MI 300

Query: 301 NDEIVEDDIHPDFK 313
           NDEIVEDDIHPDFK
Sbjct: 301 NDEIVEDDIHPDFK 305

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BLAST of Lsi08G005860.1 vs. ExPASy TrEMBL
Match: A0A0A0KCJ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G052770 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.3e-131
Identity = 249/314 (79.30%), Postives = 268/314 (85.35%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQRKSGNKWICAVCN+KQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MATIFIAVQCFQCSTMQVKQQRKSGNKWICAVCNQKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYA+EQA VDEL+DGGI+DDDKL CS RRK R+DW++YLDP E RS SG ENE ELE
Sbjct: 61  MSRKYANEQANVDELIDGGIVDDDKLVCSGRRKSRSDWSDYLDPVEDRSHSGRENEVELE 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SD    KIVTELP++KT KSGS SCSSWSE GEGDG YRP+FSKRKHIA NLSSQDEI+N
Sbjct: 121 SDL---KIVTELPDIKTWKSGSRSCSSWSEEGEGDGLYRPLFSKRKHIANNLSSQDEIRN 180

Query: 181 WESPSAIHALKWSKDDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIEESN 240
           WESPSAIHA K SK DDEQ  S  K LSAPFQDKK+ KN HK+ DEIQNWELPS+IE S 
Sbjct: 181 WESPSAIHASKLSKGDDEQTLSTAKYLSAPFQDKKMGKNGHKVEDEIQNWELPSEIEASK 240

Query: 241 WSKGISKDDESARTKTCILAKVRG-PSKWSEYIT-EENCGFRNGENMATEQGVDNLKMMI 300
           W    SKDDE+ RTK+CIL KVRG  SKWSEYIT EENCG RN ENMA E+G+D LKMMI
Sbjct: 241 W----SKDDENLRTKSCILGKVRGASSKWSEYITEEENCGGRNEENMAREEGMDVLKMMI 300

Query: 301 NDEIVEDDIHPDFK 313
           N+EIVEDDIHPDFK
Sbjct: 301 NEEIVEDDIHPDFK 307

[Close]

BLAST of Lsi08G005860.1 vs. ExPASy TrEMBL
Match: A0A6J1FH51 (MRN complex-interacting protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445345 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 1.1e-98
Identity = 212/318 (66.67%), Postives = 232/318 (72.96%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQR+SGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MSTIFIAVQCFQCSTMQVKQQRRSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYADEQ KVDE+LD  I+D+D+LD + +RKRR+DW+EYLD EE RSQ+G ENEREL 
Sbjct: 61  MSRKYADEQTKVDEILDEEIVDEDQLDFNAKRKRRSDWSEYLDLEEDRSQTGRENERELG 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SDF DGK VTELPE KTRK  STSCSS  EG EG  +YRPVF KR HIAKN         
Sbjct: 121 SDFADGKFVTELPEEKTRKHCSTSCSSRPEGVEGKRFYRPVFPKRTHIAKN--------- 180

Query: 181 WESPSAIHALKWSK---DDDEQIFSKVK-NLSAPFQDKKLRKNSHKIGDEIQNWELPSDI 240
               SAI A KW K   +DDE++  K K +LS+               DEI+NWE PS  
Sbjct: 181 ----SAIQASKWRKGVREDDEKVSLKAKHHLSSK--------------DEIRNWESPS-- 240

Query: 241 EESNWSKGISKDDESARTKTCILAKVRGP-SKWSEYITE-ENCGFRNGENMATEQGVDNL 300
                    SKDDESA    CILAKV GP SKWSEYITE +NCG R GENM TEQ +D L
Sbjct: 241 ---------SKDDESA----CILAKVGGPSSKWSEYITEDDNCGGRKGENMGTEQDMDTL 276

Query: 301 KMMINDEIVEDDIHPDFK 313
           KM+ NDEIVEDDIHPDFK
Sbjct: 301 KMITNDEIVEDDIHPDFK 276

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BLAST of Lsi08G005860.1 vs. ExPASy TrEMBL
Match: A0A6J1JWE4 (MRN complex-interacting protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488952 PE=4 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 4.1e-96
Identity = 206/317 (64.98%), Postives = 230/317 (72.56%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQR+SGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MSTIFIAVQCFQCSTMQVKQQRRSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYADEQ KVDE+ D  I+D+D+LD + +RKRR+DW+EYLD EE RSQ+G ENEREL 
Sbjct: 61  MSRKYADEQTKVDEIFDEEIVDEDQLDFNEKRKRRSDWSEYLDLEEDRSQTGRENERELG 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SDF DGKIVTELPE KTRK  STSCS+  E GEG  +YRPVF KR HIAKN         
Sbjct: 121 SDFADGKIVTELPEEKTRKHCSTSCSNRPESGEGKIFYRPVFPKRTHIAKN--------- 180

Query: 181 WESPSAIHALKWSK---DDDEQIFSKVK-NLSAPFQDKKLRKNSHKIGDEIQNWELPSDI 240
               SAI A KWSK   +DDE++  K K +LS+               DEI+NWE  S  
Sbjct: 181 ----SAIQASKWSKGVREDDEKVSLKAKHHLSSK--------------DEIRNWESLS-- 240

Query: 241 EESNWSKGISKDDESARTKTCILAKVRGPSKWSEYITE-ENCGFRNGENMATEQGVDNLK 300
                    +KDDE+A       +KV GPSKWSEYITE +NCG R GENM TEQ +D LK
Sbjct: 241 ---------TKDDENA------CSKVGGPSKWSEYITEDDNCGGRKGENMGTEQDMDTLK 273

Query: 301 MMINDEIVEDDIHPDFK 313
           M+INDEIVEDDIHPDFK
Sbjct: 301 MIINDEIVEDDIHPDFK 273

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BLAST of Lsi08G005860.1 vs. NCBI nr
Match: XP_038885326.1 (MRN complex-interacting protein [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 5.2e-138
Identity = 254/314 (80.89%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQRKSGN+WICAVCNEKQS+QKVFARA MAKDVRKFVQ+FN
Sbjct: 1   MATIFIAVQCFQCSTMQVKQQRKSGNRWICAVCNEKQSLQKVFARASMAKDVRKFVQNFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYADEQAK DELLDGGI+D+DKLDCS RRKRRNDW+EYLDPEE RS +G ENE ELE
Sbjct: 61  MSRKYADEQAKADELLDGGIVDEDKLDCSARRKRRNDWSEYLDPEEDRSHNGRENEGELE 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           S+ TD KIVTE+PEVKTRKSGSTSCS WSE GEGDG+YRP+FSKRKHIA NLSSQDE++N
Sbjct: 121 SESTDEKIVTEIPEVKTRKSGSTSCSRWSE-GEGDGFYRPIFSKRKHIANNLSSQDEVRN 180

Query: 181 WESPSAIHALKWSKDDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIEESN 240
           WES S I  +KWSK +DEQI SK KNLSAPFQDKK+ KNSHKI DEIQNWEL S+IE S 
Sbjct: 181 WESTSTIQTMKWSKGNDEQIISKAKNLSAPFQDKKMSKNSHKIADEIQNWELQSEIEASK 240

Query: 241 WSKGISKDDESARTKTCILAKVRGP-SKWSEYITEE-NCGFRNGENMATEQGVDNLKMMI 300
            SK +SKDDE++RTKT ILAKVRGP SKWSEYITEE NC  RNGENMAT+Q +D LK +I
Sbjct: 241 GSKDMSKDDENSRTKTSILAKVRGPSSKWSEYITEEDNCRGRNGENMATDQCIDILKKII 300

Query: 301 NDEIVEDDIHPDFK 313
           NDEIV+DDIHPDFK
Sbjct: 301 NDEIVDDDIHPDFK 313

[Close]

BLAST of Lsi08G005860.1 vs. NCBI nr
Match: XP_008456719.1 (PREDICTED: UPF0544 protein C5orf45 homolog [Cucumis melo] >KAA0031637.1 UPF0544 protein C5orf45-like protein [Cucumis melo var. makuwa] >TYK04473.1 UPF0544 protein C5orf45-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 480.3 bits (1235), Expect = 1.2e-131
Identity = 253/314 (80.57%), Postives = 270/314 (85.99%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MATIFIAVQCFQCSTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYA+EQA VD+L+ GGI+DDDKLDCS RRKRRNDW+EYLDPEE RS SG ENE ELE
Sbjct: 61  MSRKYANEQANVDDLIHGGIVDDDKLDCSGRRKRRNDWSEYLDPEEDRSHSGRENEGELE 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SD    KI TELP++KT KSGS SCSSWSE GEGDG YRP+FSKRKHIAKNLSSQDEI+N
Sbjct: 121 SDL---KIATELPDIKTWKSGSRSCSSWSE-GEGDGLYRPLFSKRKHIAKNLSSQDEIQN 180

Query: 181 WESPSAIHALKWSKDDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIEESN 240
           WESPSAIH  K SK DDEQ FS  K LSAPFQDKK+ KNSHK+  EIQNWELPS+IE S 
Sbjct: 181 WESPSAIHTSKLSKGDDEQTFSTAKYLSAPFQDKKMSKNSHKVESEIQNWELPSEIEASK 240

Query: 241 WSKGISKDDESARTKTCILAKVRG-PSKWSEYIT-EENCGFRNGENMATEQGVDNLKMMI 300
           W    +KDDE++RTK+CIL KVRG  SKWSEYIT EENCG RN ENMA EQG+D LK MI
Sbjct: 241 W----TKDDENSRTKSCILGKVRGASSKWSEYITEEENCGGRNEENMAREQGMDILK-MI 300

Query: 301 NDEIVEDDIHPDFK 313
           NDEIVEDDIHPDFK
Sbjct: 301 NDEIVEDDIHPDFK 305

[Close]

BLAST of Lsi08G005860.1 vs. NCBI nr
Match: XP_004141004.1 (MRN complex-interacting protein [Cucumis sativus] >KGN46102.1 hypothetical protein Csa_005258 [Cucumis sativus])

HSP 1 Score: 478.4 bits (1230), Expect = 4.7e-131
Identity = 249/314 (79.30%), Postives = 268/314 (85.35%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQRKSGNKWICAVCN+KQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MATIFIAVQCFQCSTMQVKQQRKSGNKWICAVCNQKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYA+EQA VDEL+DGGI+DDDKL CS RRK R+DW++YLDP E RS SG ENE ELE
Sbjct: 61  MSRKYANEQANVDELIDGGIVDDDKLVCSGRRKSRSDWSDYLDPVEDRSHSGRENEVELE 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SD    KIVTELP++KT KSGS SCSSWSE GEGDG YRP+FSKRKHIA NLSSQDEI+N
Sbjct: 121 SDL---KIVTELPDIKTWKSGSRSCSSWSEEGEGDGLYRPLFSKRKHIANNLSSQDEIRN 180

Query: 181 WESPSAIHALKWSKDDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIEESN 240
           WESPSAIHA K SK DDEQ  S  K LSAPFQDKK+ KN HK+ DEIQNWELPS+IE S 
Sbjct: 181 WESPSAIHASKLSKGDDEQTLSTAKYLSAPFQDKKMGKNGHKVEDEIQNWELPSEIEASK 240

Query: 241 WSKGISKDDESARTKTCILAKVRG-PSKWSEYIT-EENCGFRNGENMATEQGVDNLKMMI 300
           W    SKDDE+ RTK+CIL KVRG  SKWSEYIT EENCG RN ENMA E+G+D LKMMI
Sbjct: 241 W----SKDDENLRTKSCILGKVRGASSKWSEYITEEENCGGRNEENMAREEGMDVLKMMI 300

Query: 301 NDEIVEDDIHPDFK 313
           N+EIVEDDIHPDFK
Sbjct: 301 NEEIVEDDIHPDFK 307

[Close]

BLAST of Lsi08G005860.1 vs. NCBI nr
Match: XP_023549686.1 (MRN complex-interacting protein isoform X2 [Cucurbita pepo subsp. pepo] >XP_023549687.1 MRN complex-interacting protein isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 386.0 bits (990), Expect = 3.2e-103
Identity = 216/316 (68.35%), Postives = 239/316 (75.63%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQR+SGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MSTIFIAVQCFQCSTMQVKQQRRSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYADEQ KVDE+LD  I+D+D+LD + +RKRR+DW+EYLD EE RSQ+G ENEREL 
Sbjct: 61  MSRKYADEQTKVDEILDEEIVDEDQLDFNAKRKRRSDWSEYLDLEEDRSQTGRENERELG 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SDFTDGKIVTELPE KTRK  STSCSS  EGGEG  +YRPVF KR HIAKN         
Sbjct: 121 SDFTDGKIVTELPEEKTRKHCSTSCSSRPEGGEGKRFYRPVFPKRTHIAKN--------- 180

Query: 181 WESPSAIHALKWSK---DDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIE 240
               SAI A KW K   +DDE++  K  NLS+               DEI+NWE  SDIE
Sbjct: 181 ----SAIQASKWRKGVREDDEKVSLKA-NLSSK--------------DEIRNWESQSDIE 240

Query: 241 ESNWSKGISKDDESARTKTCILAKVRGPS-KWSEYITEENCGFRNGENMATEQGVDNLKM 300
            S WSKG+SKDDE+A    CILAKV GPS KWSEY+TEE+      +NM TEQ +D LKM
Sbjct: 241 ASKWSKGMSKDDENA----CILAKVGGPSPKWSEYMTEED------KNMGTEQDMDTLKM 278

Query: 301 MINDEIVEDDIHPDFK 313
           +INDEIVEDDIHPDFK
Sbjct: 301 IINDEIVEDDIHPDFK 278

[Close]

BLAST of Lsi08G005860.1 vs. NCBI nr
Match: XP_023549685.1 (MRN complex-interacting protein isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.6 bits (971), Expect = 5.1e-101
Identity = 217/332 (65.36%), Postives = 240/332 (72.29%), Query Frame = 0


Query: 1   METIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60
           M TIFIAVQCFQC TMQVKQQR+SGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN
Sbjct: 1   MSTIFIAVQCFQCSTMQVKQQRRSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFN 60

Query: 61  MSRKYADEQAKVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELE 120
           MSRKYADEQ KVDE+LD  I+D+D+LD + +RKRR+DW+EYLD EE RSQ+G ENEREL 
Sbjct: 61  MSRKYADEQTKVDEILDEEIVDEDQLDFNAKRKRRSDWSEYLDLEEDRSQTGRENERELG 120

Query: 121 SDFTDGKIVTELPEVKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLSSQDEIKN 180
           SDFTDGKIVTELPE KTRK  STSCSS  EGGEG  +YRPVF KR HIAKN         
Sbjct: 121 SDFTDGKIVTELPEEKTRKHCSTSCSSRPEGGEGKRFYRPVFPKRTHIAKN--------- 180

Query: 181 WESPSAIHALKWSK---DDDEQIFSKVKNLSAPFQDKKLRKNSHKIGDEIQNWELPSDIE 240
               SAI A KW K   +DDE++  K  NLS+               DEI+NWE  SDIE
Sbjct: 181 ----SAIQASKWRKGVREDDEKVSLKA-NLSSK--------------DEIRNWESQSDIE 240

Query: 241 ESNWSKGISKDDESARTKTCILAKVRGPS-KWSEYITEE--NCGFRNG------------ 300
            S WSKG+SKDDE+A    CILAKV GPS KWSEY+TEE  N G                
Sbjct: 241 ASKWSKGMSKDDENA----CILAKVGGPSPKWSEYMTEEDKNMGTEQDMDTLKMIINDEI 300

Query: 301 --ENMATEQGVDNLKMMINDEIVEDDIHPDFK 313
             ++M TEQ +D LKM+INDEIVEDDIHPDFK
Sbjct: 301 VEDDMGTEQDMDTLKMIINDEIVEDDIHPDFK 300

[Close]

BLAST of Lsi08G005860.1 vs. TAIR 10
Match: AT1G71760.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: shoot apex, embryo, leaf whorl, flower, seed; EXPRESSED DURING: F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; Has 84 Blast hits to 84 proteins in 35 species: Archae - 0; Bacteria - 0; Metazoa - 42; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 124.0 bits (310), Expect = 2.1e-28
Identity = 71/172 (41.28%), Postives = 107/172 (62.21%), Query Frame = 0


Query: 3   TIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFNMS 62
           T+FIA+QC++C TMQVKQ++KS NKW+C +CN+KQSV+KVFA+   AK++R FVQSFNMS
Sbjct: 4   TLFIALQCYECSTMQVKQKKKSSNKWVCVICNQKQSVKKVFAQGYKAKELRFFVQSFNMS 63

Query: 63  RKYADEQA-KVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELES 122
           RK ADE+A  V +      ++ +  +     K+R+DW++YLD +         + R L  
Sbjct: 64  RKVADEEAHAVGDSFPEVYVEGEVNEEVNGTKKRSDWSQYLDFD------SVNDRRRLAG 123

Query: 123 DFTDGKIVTELPE--------VKTRKSGSTSCSSWSEGGEGDGYYRPVFSKR 166
           +  D KIVTE+P          K   +G +S ++  E  + +  ++P FS+R
Sbjct: 124 EEDDVKIVTEMPSNMFKRPKLNKESNAGGSSSNTGGENKDSNEIFKPSFSRR 169

[Close]

BLAST of Lsi08G005860.1 vs. TAIR 10
Match: AT1G71760.1 (unknown protein; Has 92 Blast hits to 92 proteins in 41 species: Archae - 0; Bacteria - 0; Metazoa - 44; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 16 (source: NCBI BLink). )

HSP 1 Score: 122.9 bits (307), Expect = 4.7e-28
Identity = 89/249 (35.74%), Postives = 132/249 (53.01%), Query Frame = 0


Query: 3   TIFIAVQCFQCFTMQVKQQRKSGNKWICAVCNEKQSVQKVFARAPMAKDVRKFVQSFNMS 62
           T+FIA+QC++C TMQVKQ++KS NKW+C +CN+KQSV+KVFA+   AK++R FVQSFNMS
Sbjct: 4   TLFIALQCYECSTMQVKQKKKSSNKWVCVICNQKQSVKKVFAQGYKAKELRFFVQSFNMS 63

Query: 63  RKYADEQA-KVDELLDGGIIDDDKLDCSVRRKRRNDWNEYLDPEEHRSQSGGENERELES 122
           RK ADE+A  V +      ++ +  +     K+R+DW++YLD +         + R L  
Sbjct: 64  RKVADEEAHAVGDSFPEVYVEGEVNEEVNGTKKRSDWSQYLDFD------SVNDRRRLAG 123

Query: 123 DFTDGKIVTELPE--------VKTRKSGSTSCSSWSEGGEGDGYYRPVFSKRKHIAKNLS 182
           +  D KIVTE+P          K   +G +S ++  E  + +  ++P FS+R     +  
Sbjct: 124 EEDDVKIVTEMPSNMFKRPKLNKESNAGGSSSNTGGENKDSNEIFKPSFSRRSIKKPDFR 183

Query: 183 SQD--------EIKNWESPSAIH-ALKWSKDDDEQIFSKVKNLSAP--FQDKKLRKNSHK 232
           S +        E +N ES      A KW    D  +        AP  F  KK  K+   
Sbjct: 184 SDEVVTRKKDMEERNLESERVTKPASKW----DAYLIDDEGEHQAPPRFGVKKALKD--- 237

The following BLAST results are available for this feature:

BLAST of Lsi08G005860.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q32PP1	2.2e-06	28.37	MRN complex-interacting protein OS=Danio rerio OX=7955 GN=mrnip PE=2 SV=1	[more]

BLAST of Lsi08G005860.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BXL1	6.0e-132	80.57	UPF0544 protein C5orf45-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3C3H7	6.0e-132	80.57	UPF0544 protein C5orf45 homolog OS=Cucumis melo OX=3656 GN=LOC103496578 PE=4 SV=...	[more]
A0A0A0KCJ3	2.3e-131	79.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G052770 PE=4 SV=1	[more]
A0A6J1FH51	1.1e-98	66.67	MRN complex-interacting protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JWE4	4.1e-96	64.98	MRN complex-interacting protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Lsi08G005860.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885326.1	5.2e-138	80.89	MRN complex-interacting protein [Benincasa hispida]	[more]
XP_008456719.1	1.2e-131	80.57	PREDICTED: UPF0544 protein C5orf45 homolog [Cucumis melo] >KAA0031637.1 UPF0544 ...	[more]
XP_004141004.1	4.7e-131	79.30	MRN complex-interacting protein [Cucumis sativus] >KGN46102.1 hypothetical prote...	[more]
XP_023549686.1	3.2e-103	68.35	MRN complex-interacting protein isoform X2 [Cucurbita pepo subsp. pepo] >XP_0235...	[more]
XP_023549685.1	5.1e-101	65.36	MRN complex-interacting protein isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi08G005860.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71760.2	2.1e-28	41.28	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G71760.1	4.7e-28	35.74	unknown protein; Has 92 Blast hits to 92 proteins in 41 species: Archae - 0; Bac...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032739	MRN complex-interacting protein	PFAM	PF15749	MRNIP	coord: 8..149 e-value: 1.6E-17 score: 64.2
IPR032739	MRN complex-interacting protein	PANTHER	PTHR15863	MRN COMPLEX-INTERACTING PROTEIN	coord: 1..311
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 103..123

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi08G005860	Lsi08G005860	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G005860.1.exon.4	Lsi08G005860.1.exon.4	exon
Lsi08G005860.1.exon.3	Lsi08G005860.1.exon.3	exon
Lsi08G005860.1.exon.2	Lsi08G005860.1.exon.2	exon
Lsi08G005860.1.exon.1	Lsi08G005860.1.exon.1	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G005860.1.five_prime_UTR.1	Lsi08G005860.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G005860.1.CDS.1	Lsi08G005860.1.CDS.1	CDS
Lsi08G005860.1.CDS.2	Lsi08G005860.1.CDS.2	CDS
Lsi08G005860.1.CDS.3	Lsi08G005860.1.CDS.3	CDS
Lsi08G005860.1.CDS.4	Lsi08G005860.1.CDS.4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G005860.1.three_prime_UTR.1	Lsi08G005860.1.three_prime_UTR.1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi08G005860.1	Lsi08G005860.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007095	mitotic G2 DNA damage checkpoint signaling
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003682	chromatin binding