Lsi08G001730.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G001730.1 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 4.3e-48
Identity = 98/197 (49.75%), Postives = 129/197 (65.48%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           LPLK RI   + S  +  ++R + T+NR  +  FD + PP+ KP N VST D V D SR+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 64  LWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVVSVNY 123
           LWFRL+ P  S       +PV+V++HGGGF F S N+ PYD++CRR AR L   V+SVNY
Sbjct: 73  LWFRLYTPHVS----GDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNY 132

Query: 124 RLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHVAV-- 183
           RL+PEH YP  Y DGFDAL +++  ++ G + P   DLSRCF AGDSAGGN+AH+VA+  
Sbjct: 133 RLAPEHRYPAQYDDGFDALKYIE--ENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 184 -RAGGYDFKKLKIKGLI 198
            R     F  +K+ GLI
Sbjct: 193 CREPRSSFTAVKLIGLI 203

BLAST of Lsi08G001730.1 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 7.4e-32
Identity = 79/190 (41.58%), Postives = 107/190 (56.32%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           +PL   +L         L RR + T NR L  F D K+P ++ P NGV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 64  LWFRLFLPSSSVVADNVT----------LPVIVYYHGGGFVFFSANSMPYDDLCRRLARD 123
           L  R++ P+ +  + ++T          +PVIV++HGG F   SANS  YD LCRRL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 124 LRVVVVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGG 183
              VVVSVNYR +PE+ YP  Y DG+  L +   ++S   +   K    R FLAGDS+GG
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKW---VNSSSWLRSKKDSKVRIFLAGDSSGG 195

BLAST of Lsi08G001730.1 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.0e-30
Identity = 70/176 (39.77%), Postives = 102/176 (57.95%), Query Frame = 0

Query: 28  TVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRNLWFRLFLPSSSVVADNVT---LPV 87
           ++ R L +F      P   P N   + D+  +  ++ W RL+LPSS+V   NV+   LP+
Sbjct: 23  SITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPI 82

Query: 88  IVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVVSVNYRLSPEHPYPIPYQDGFDALNF 147
           +VYYHGGGF+  S +   + D C  +ARDL  +VVS +YRL+PEH  P  Y DG +AL++
Sbjct: 83  VVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDW 142

Query: 148 LDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHVAVRA--GGYDFKKLKIKGLII 199
           +   D +        D S  FL G SAGGNLA++V +R+     D   L+I+GLI+
Sbjct: 143 IKTSDDEW--IKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLIL 196

BLAST of Lsi08G001730.1 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.9e-30
Identity = 68/160 (42.50%), Postives = 99/160 (61.88%), Query Frame = 0

Query: 39  PKIPPSSKPHNGVSTHDVVFDPSRNLWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSA 98
           P + PS     GV+  DVV D   N+W RL++P ++  +    LP+IVY+HGGGF   SA
Sbjct: 46  PCVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTTKSSVSKLPLIVYFHGGGFCVGSA 105

Query: 99  NSMPYDDLCRRLARDLRVVVVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVK 158
           + + Y +   RL+   R +V+SVNYRL+PE+P P  Y+DG +A+ +L+   +D  ++  +
Sbjct: 106 SWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYEDGVNAILWLNKARND-NLWAKQ 165

Query: 159 LDLSRCFLAGDSAGGNLAHHVAVRAGGYDFKKLKIKGLII 199
            D  R FLAGDSAGGN+A  VA R    +   LKI+G I+
Sbjct: 166 CDFGRIFLAGDSAGGNIAQQVAARLASPEDLALKIEGTIL 204

BLAST of Lsi08G001730.1 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.9e-30
Identity = 78/193 (40.41%), Postives = 110/193 (56.99%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           +PL   +L         + RR + T NR L  + D K+  ++ P +GV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 64  LWFRLFLPS-------SSVV-----ADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLA 123
           L  R++ P+        S++      D   +PVI+++HGG F   SANS  YD LCRRL 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 124 RDLRVVVVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSA 183
              + VVVSVNYR +PE+PYP  Y DG+ ALN+   ++S   +   K      FLAGDS+
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNW---VNSRSWLKSKKDSKVHIFLAGDSS 195

Query: 184 GGNLAHHVAVRAG 185
           GGN+AH+VA+RAG
Sbjct: 196 GGNIAHNVALRAG 205

BLAST of Lsi08G001730.1 vs. ExPASy TrEMBL
Match: A0A1S3CAV2 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498628 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.7e-87
Identity = 160/198 (80.81%), Postives = 169/198 (85.35%), Query Frame = 0

Query: 1   MENLPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDP 60
           ME LPLKFRIL   HSF S +SRRSN+TVNR  MSFFDPK  PSSKP NGVST+DVVFDP
Sbjct: 1   MEKLPLKFRILLHFHSFASSISRRSNVTVNRFFMSFFDPKFSPSSKPRNGVSTYDVVFDP 60

Query: 61  SRNLWFRLFLPSSSVVADNVT-LPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVV 120
           S NLWFRLFLPSSS    NVT LPV+VYYHGGGFVFFSANSM YDD CRRLAR+LRV VV
Sbjct: 61  SHNLWFRLFLPSSSSADKNVTDLPVVVYYHGGGFVFFSANSMAYDDFCRRLARELRVAVV 120

Query: 121 SVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHV 180
           SVNYRLSPEH  PIPY+DGFDAL FLD MD D G FPVK D+SRCFLAGDSAGGNL HHV
Sbjct: 121 SVNYRLSPEHRCPIPYEDGFDALKFLDGMDLDDGGFPVKFDVSRCFLAGDSAGGNLTHHV 180

Query: 181 AVRAGGYDFKKLKIKGLI 198
           AVRAGGY+FKKLKIKG+I
Sbjct: 181 AVRAGGYNFKKLKIKGII 198

BLAST of Lsi08G001730.1 vs. ExPASy TrEMBL
Match: A0A5D3BNH2 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001400 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 1.9e-86
Identity = 159/196 (81.12%), Postives = 167/196 (85.20%), Query Frame = 0

Query: 1   MENLPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDP 60
           ME LPLKFRIL   HSF S +SRRSN+TVNR  MSFFDPK  PSSKP NGVST+DVVFDP
Sbjct: 1   MEKLPLKFRILLHFHSFASSISRRSNVTVNRFFMSFFDPKFSPSSKPRNGVSTYDVVFDP 60

Query: 61  SRNLWFRLFLPSSSVVADNVT-LPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVV 120
           S NLWFRLFLPSSS    NVT LPV+VYYHGGGFVFFSANSM YDD CRRLAR+LRV VV
Sbjct: 61  SHNLWFRLFLPSSSSADKNVTDLPVVVYYHGGGFVFFSANSMAYDDFCRRLARELRVAVV 120

Query: 121 SVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHV 180
           SVNYRLSPEH  PIPY+DGFDAL FLD MD D G FPVK D+SRCFLAGDSAGGNL HHV
Sbjct: 121 SVNYRLSPEHRCPIPYEDGFDALKFLDGMDLDDGGFPVKFDVSRCFLAGDSAGGNLTHHV 180

Query: 181 AVRAGGYDFKKLKIKG 196
           AVRAGGY+FKKLKIKG
Sbjct: 181 AVRAGGYNFKKLKIKG 196

BLAST of Lsi08G001730.1 vs. ExPASy TrEMBL
Match: A0A6J1I994 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472693 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.2e-86
Identity = 158/200 (79.00%), Postives = 173/200 (86.50%), Query Frame = 0

Query: 1   MEN---LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVV 60
           MEN   LPLKF+IL RLHSF S + RR NITVNRCL + FDPK  PSSKP+NGVSTHD+V
Sbjct: 1   MENPPKLPLKFQILLRLHSFASSIGRRPNITVNRCLTNLFDPKFSPSSKPNNGVSTHDIV 60

Query: 61  FDPSRNLWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVV 120
           FDPSRNLWFRLF+PSS  V+D+V+LPV+VY+HGGGFVFFSANSMPYDDL RRLARDLRVV
Sbjct: 61  FDPSRNLWFRLFVPSS--VSDDVSLPVVVYFHGGGFVFFSANSMPYDDLGRRLARDLRVV 120

Query: 121 VVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAH 180
           VVSVNYRLSPEH YP+PY+DGFD L FLD+M  D  VFP K DL RCFLAGDSAGGNLAH
Sbjct: 121 VVSVNYRLSPEHRYPLPYEDGFDGLKFLDEMGFDSVVFPAKADLGRCFLAGDSAGGNLAH 180

Query: 181 HVAVRAGGYDFKKLKIKGLI 198
           HVAVRA GYDFKK+ IKGLI
Sbjct: 181 HVAVRAAGYDFKKVNIKGLI 198

BLAST of Lsi08G001730.1 vs. ExPASy TrEMBL
Match: A0A0A0KUP0 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647320 PE=4 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.7e-85
Identity = 159/200 (79.50%), Postives = 170/200 (85.00%), Query Frame = 0

Query: 1   MENLPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDP 60
           ME LP KFRIL    SF SF+SRRSN+TVNR LMS FDPK   SSKP +GVST+DVVFDP
Sbjct: 1   MERLPFKFRILLYFFSFASFISRRSNVTVNRFLMSLFDPKYSASSKPRHGVSTYDVVFDP 60

Query: 61  SRNLWFRLFLPSSSVVADN---VTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVV 120
           S NLWFRLFLPSSS   DN     LPVIVYYHGGGFVFFSANSM YDDLCRRLAR+LRV 
Sbjct: 61  SHNLWFRLFLPSSSSSTDNNNVTDLPVIVYYHGGGFVFFSANSMAYDDLCRRLARELRVA 120

Query: 121 VVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAH 180
           VVSVNYRLSPEH  PIPY+DGFDAL +LD MD DGG FPVKLD+SRCFLAGDSAGGNLAH
Sbjct: 121 VVSVNYRLSPEHRCPIPYEDGFDALKYLDGMDLDGGGFPVKLDVSRCFLAGDSAGGNLAH 180

Query: 181 HVAVRAGGYDFKKLKIKGLI 198
           HVAVRAGG++FKKLKIKG+I
Sbjct: 181 HVAVRAGGHNFKKLKIKGII 200

BLAST of Lsi08G001730.1 vs. ExPASy TrEMBL
Match: A0A5D3BQZ2 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001390 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 5.6e-83
Identity = 157/198 (79.29%), Postives = 171/198 (86.36%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           LP K R+L RL S  SF+SRRSNITVNR L+SFFDPKIP SSKPHNGVST+DVVFD SRN
Sbjct: 9   LPWKSRLLIRLFSSASFISRRSNITVNRFLISFFDPKIPSSSKPHNGVSTYDVVFDHSRN 68

Query: 64  LWFRLFLP--SSSVVADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVVSV 123
           LWFR FLP  SSS  +D VTLPV+VYYHGGGFVFFS +SM YDDLCRRLAR+L  VV SV
Sbjct: 69  LWFRFFLPSSSSSSSSDGVTLPVVVYYHGGGFVFFSPDSMAYDDLCRRLARELGAVVASV 128

Query: 124 NYRLSPEHPYPIPYQDGFDALNFLDDMD--SDGGVFPVKLDLSRCFLAGDSAGGNLAHHV 183
           NYRLSPEH +PIPY+DGFDAL FLD MD  SD G FPVKLD+SRCFLAGDSAGGNLAHHV
Sbjct: 129 NYRLSPEHRFPIPYEDGFDALKFLDTMDNNSDFGGFPVKLDVSRCFLAGDSAGGNLAHHV 188

Query: 184 AVRAGGYDFKKLKIKGLI 198
           A+R+GGYDFKK+KIKGLI
Sbjct: 189 ALRSGGYDFKKVKIKGLI 206

BLAST of Lsi08G001730.1 vs. NCBI nr
Match: XP_038889726.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 345.1 bits (884), Expect = 4.0e-91
Identity = 164/194 (84.54%), Postives = 179/194 (92.27%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           LPLKFRIL RLHSF SF+SRR NITVNR LMSFFDPKIPPSSKP  GV+T+DVVFDPSRN
Sbjct: 7   LPLKFRILLRLHSFASFISRRPNITVNRFLMSFFDPKIPPSSKPRYGVTTNDVVFDPSRN 66

Query: 64  LWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVVSVNY 123
           LWFRLFLPSSSV+ADN++LPV+VYYHGGGFVFF ANSM YDDLCRRLAR+LRVVVVSVNY
Sbjct: 67  LWFRLFLPSSSVLADNISLPVVVYYHGGGFVFFWANSMAYDDLCRRLARELRVVVVSVNY 126

Query: 124 RLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHVAVRA 183
           RLSPEH YPIPY+DGFDAL FLD +D + GVFPV +D+SRCFLAGDSAGGNLAHHVAVRA
Sbjct: 127 RLSPEHRYPIPYEDGFDALKFLDGVDLNCGVFPVNVDVSRCFLAGDSAGGNLAHHVAVRA 186

Query: 184 GGYDFKKLKIKGLI 198
           G +DFKK+KIKGLI
Sbjct: 187 GSHDFKKVKIKGLI 200

BLAST of Lsi08G001730.1 vs. NCBI nr
Match: XP_038890672.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 338.2 bits (866), Expect = 4.8e-89
Identity = 163/197 (82.74%), Postives = 173/197 (87.82%), Query Frame = 0

Query: 1   MENLPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDP 60
           ME LPLKFRIL RL S  SF+SRR NITVNR LMSFFDPK PPSSKP NGVST+DVVFDP
Sbjct: 3   MEKLPLKFRILLRLRSIASFISRRPNITVNRFLMSFFDPKTPPSSKPRNGVSTNDVVFDP 62

Query: 61  SRNLWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVVS 120
            RNLWFRLFLPSSSV+ADNV+LPV+VYYHGGGFVFFSANSMPYDD CRRLARDLRVVVVS
Sbjct: 63  FRNLWFRLFLPSSSVLADNVSLPVVVYYHGGGFVFFSANSMPYDDFCRRLARDLRVVVVS 122

Query: 121 VNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHVA 180
           VNYRLSPEH YPIPY+DGFDAL FLD  D    VFP  +D  +CF+AGDSAGGNLAH VA
Sbjct: 123 VNYRLSPEHRYPIPYEDGFDALKFLDGTDL---VFPANVDFDQCFIAGDSAGGNLAHQVA 182

Query: 181 VRAGGYDFKKLKIKGLI 198
           V+AGGYDFKKLKIKGLI
Sbjct: 183 VKAGGYDFKKLKIKGLI 196

BLAST of Lsi08G001730.1 vs. NCBI nr
Match: XP_008459512.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 330.9 bits (847), Expect = 7.7e-87
Identity = 160/198 (80.81%), Postives = 169/198 (85.35%), Query Frame = 0

Query: 1   MENLPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDP 60
           ME LPLKFRIL   HSF S +SRRSN+TVNR  MSFFDPK  PSSKP NGVST+DVVFDP
Sbjct: 1   MEKLPLKFRILLHFHSFASSISRRSNVTVNRFFMSFFDPKFSPSSKPRNGVSTYDVVFDP 60

Query: 61  SRNLWFRLFLPSSSVVADNVT-LPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVV 120
           S NLWFRLFLPSSS    NVT LPV+VYYHGGGFVFFSANSM YDD CRRLAR+LRV VV
Sbjct: 61  SHNLWFRLFLPSSSSADKNVTDLPVVVYYHGGGFVFFSANSMAYDDFCRRLARELRVAVV 120

Query: 121 SVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHV 180
           SVNYRLSPEH  PIPY+DGFDAL FLD MD D G FPVK D+SRCFLAGDSAGGNL HHV
Sbjct: 121 SVNYRLSPEHRCPIPYEDGFDALKFLDGMDLDDGGFPVKFDVSRCFLAGDSAGGNLTHHV 180

Query: 181 AVRAGGYDFKKLKIKGLI 198
           AVRAGGY+FKKLKIKG+I
Sbjct: 181 AVRAGGYNFKKLKIKGII 198

BLAST of Lsi08G001730.1 vs. NCBI nr
Match: KAA0039347.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00530.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 328.6 bits (841), Expect = 3.8e-86
Identity = 159/196 (81.12%), Postives = 167/196 (85.20%), Query Frame = 0

Query: 1   MENLPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDP 60
           ME LPLKFRIL   HSF S +SRRSN+TVNR  MSFFDPK  PSSKP NGVST+DVVFDP
Sbjct: 1   MEKLPLKFRILLHFHSFASSISRRSNVTVNRFFMSFFDPKFSPSSKPRNGVSTYDVVFDP 60

Query: 61  SRNLWFRLFLPSSSVVADNVT-LPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVV 120
           S NLWFRLFLPSSS    NVT LPV+VYYHGGGFVFFSANSM YDD CRRLAR+LRV VV
Sbjct: 61  SHNLWFRLFLPSSSSADKNVTDLPVVVYYHGGGFVFFSANSMAYDDFCRRLARELRVAVV 120

Query: 121 SVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHV 180
           SVNYRLSPEH  PIPY+DGFDAL FLD MD D G FPVK D+SRCFLAGDSAGGNL HHV
Sbjct: 121 SVNYRLSPEHRCPIPYEDGFDALKFLDGMDLDDGGFPVKFDVSRCFLAGDSAGGNLTHHV 180

Query: 181 AVRAGGYDFKKLKIKG 196
           AVRAGGY+FKKLKIKG
Sbjct: 181 AVRAGGYNFKKLKIKG 196

BLAST of Lsi08G001730.1 vs. NCBI nr
Match: XP_022974072.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 327.8 bits (839), Expect = 6.5e-86
Identity = 158/200 (79.00%), Postives = 173/200 (86.50%), Query Frame = 0

Query: 1   MEN---LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVV 60
           MEN   LPLKF+IL RLHSF S + RR NITVNRCL + FDPK  PSSKP+NGVSTHD+V
Sbjct: 1   MENPPKLPLKFQILLRLHSFASSIGRRPNITVNRCLTNLFDPKFSPSSKPNNGVSTHDIV 60

Query: 61  FDPSRNLWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVV 120
           FDPSRNLWFRLF+PSS  V+D+V+LPV+VY+HGGGFVFFSANSMPYDDL RRLARDLRVV
Sbjct: 61  FDPSRNLWFRLFVPSS--VSDDVSLPVVVYFHGGGFVFFSANSMPYDDLGRRLARDLRVV 120

Query: 121 VVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAH 180
           VVSVNYRLSPEH YP+PY+DGFD L FLD+M  D  VFP K DL RCFLAGDSAGGNLAH
Sbjct: 121 VVSVNYRLSPEHRYPLPYEDGFDGLKFLDEMGFDSVVFPAKADLGRCFLAGDSAGGNLAH 180

Query: 181 HVAVRAGGYDFKKLKIKGLI 198
           HVAVRA GYDFKK+ IKGLI
Sbjct: 181 HVAVRAAGYDFKKVNIKGLI 198

BLAST of Lsi08G001730.1 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 192.6 bits (488), Expect = 3.1e-49
Identity = 98/197 (49.75%), Postives = 129/197 (65.48%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           LPLK RI   + S  +  ++R + T+NR  +  FD + PP+ KP N VST D V D SR+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 64  LWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVVSVNY 123
           LWFRL+ P  S       +PV+V++HGGGF F S N+ PYD++CRR AR L   V+SVNY
Sbjct: 73  LWFRLYTPHVS----GDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNY 132

Query: 124 RLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHVAV-- 183
           RL+PEH YP  Y DGFDAL +++  ++ G + P   DLSRCF AGDSAGGN+AH+VA+  
Sbjct: 133 RLAPEHRYPAQYDDGFDALKYIE--ENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 184 -RAGGYDFKKLKIKGLI 198
            R     F  +K+ GLI
Sbjct: 193 CREPRSSFTAVKLIGLI 203

BLAST of Lsi08G001730.1 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 138.7 bits (348), Expect = 5.2e-33
Identity = 79/190 (41.58%), Postives = 107/190 (56.32%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           +PL   +L         L RR + T NR L  F D K+P ++ P NGV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 64  LWFRLFLPSSSVVADNVT----------LPVIVYYHGGGFVFFSANSMPYDDLCRRLARD 123
           L  R++ P+ +  + ++T          +PVIV++HGG F   SANS  YD LCRRL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 124 LRVVVVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSAGG 183
              VVVSVNYR +PE+ YP  Y DG+  L +   ++S   +   K    R FLAGDS+GG
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKW---VNSSSWLRSKKDSKVRIFLAGDSSGG 195

BLAST of Lsi08G001730.1 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 132.9 bits (333), Expect = 2.9e-31
Identity = 70/176 (39.77%), Postives = 102/176 (57.95%), Query Frame = 0

Query: 28  TVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRNLWFRLFLPSSSVVADNVT---LPV 87
           ++ R L +F      P   P N   + D+  +  ++ W RL+LPSS+V   NV+   LP+
Sbjct: 23  SITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPI 82

Query: 88  IVYYHGGGFVFFSANSMPYDDLCRRLARDLRVVVVSVNYRLSPEHPYPIPYQDGFDALNF 147
           +VYYHGGGF+  S +   + D C  +ARDL  +VVS +YRL+PEH  P  Y DG +AL++
Sbjct: 83  VVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDW 142

Query: 148 LDDMDSDGGVFPVKLDLSRCFLAGDSAGGNLAHHVAVRA--GGYDFKKLKIKGLII 199
           +   D +        D S  FL G SAGGNLA++V +R+     D   L+I+GLI+
Sbjct: 143 IKTSDDEW--IKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLIL 196

BLAST of Lsi08G001730.1 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.9e-31
Identity = 68/160 (42.50%), Postives = 99/160 (61.88%), Query Frame = 0

Query: 39  PKIPPSSKPHNGVSTHDVVFDPSRNLWFRLFLPSSSVVADNVTLPVIVYYHGGGFVFFSA 98
           P + PS     GV+  DVV D   N+W RL++P ++  +    LP+IVY+HGGGF   SA
Sbjct: 46  PCVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTTKSSVSKLPLIVYFHGGGFCVGSA 105

Query: 99  NSMPYDDLCRRLARDLRVVVVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVK 158
           + + Y +   RL+   R +V+SVNYRL+PE+P P  Y+DG +A+ +L+   +D  ++  +
Sbjct: 106 SWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYEDGVNAILWLNKARND-NLWAKQ 165

Query: 159 LDLSRCFLAGDSAGGNLAHHVAVRAGGYDFKKLKIKGLII 199
            D  R FLAGDSAGGN+A  VA R    +   LKI+G I+
Sbjct: 166 CDFGRIFLAGDSAGGNIAQQVAARLASPEDLALKIEGTIL 204

BLAST of Lsi08G001730.1 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.9e-31
Identity = 78/193 (40.41%), Postives = 110/193 (56.99%), Query Frame = 0

Query: 4   LPLKFRILFRLHSFGSFLSRRSNITVNRCLMSFFDPKIPPSSKPHNGVSTHDVVFDPSRN 63
           +PL   +L         + RR + T NR L  + D K+  ++ P +GV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 64  LWFRLFLPS-------SSVV-----ADNVTLPVIVYYHGGGFVFFSANSMPYDDLCRRLA 123
           L  R++ P+        S++      D   +PVI+++HGG F   SANS  YD LCRRL 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 124 RDLRVVVVSVNYRLSPEHPYPIPYQDGFDALNFLDDMDSDGGVFPVKLDLSRCFLAGDSA 183
              + VVVSVNYR +PE+PYP  Y DG+ ALN+   ++S   +   K      FLAGDS+
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNW---VNSRSWLKSKKDSKVHIFLAGDSS 195

Query: 184 GGNLAHHVAVRAG 185
           GGN+AH+VA+RAG
Sbjct: 196 GGNIAHNVALRAG 205