Lsi05G005940.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G005940.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncalcium-dependent protein kinase-like
Locationchr05: 7378496 .. 7381745 (+)
Sequence length1062
RNA-Seq ExpressionLsi05G005940.1
SyntenyLsi05G005940.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGAAAAGTTACAAGTTTTATCTTCGGTTGTTTGTGGTATTCATGTACTTATCCAACAATGACAGATAAGAGCTAGTCAACCTCAATATAAAAACAAAATATTGGTAGAGATCAACTTGTAAGTATTCAATTCACATGGGTATAAGAACTATTCTTCTTGGTCAAGGTTCATCTATATGGATTGATAACCTCAACCTTGAAGGCTTGAACTTCCCTGGGTATTTTCTTCATCAACTAAAGCAGCTAGGTGGCTAGGTTTGACAAGTACATAACTCTTGTAGCACGTTTGTCGTCTTTTCGTTTTATTCATTTCAACAGTTGTTAATGCCTTCATCGTGACAGAATTGTCAACCAATGTTGAATTGTCTAAATCTCTTCAATTTATCATTAATTTCTTTTCTTGAAATGGATTGTTGATGCGTTTATTTCCTGACGTGGATGGCCTCAAACATGATTAACTTTTGTTTTGCGATTTTTAATGTTTTTTTTCTTAATATTATGAGGATAAAGAGCAGCATTTTAACCTTGGACGTTTTGTATAATGCATATACTATCTGATTGTCGATTGGTTCTCTGTTTTTCTCAATAATTCTTGCTTTGATTTCTATATGTAGTTTTTCCTGTGAATTGCTTTTAGTGTGAAGGAGAGAAGTAATGGGATCATATACTTAAAATTTATCTGCCTGATACTTCAAATAGAACAATTTATGTTATCATTTTAGCAAGACCATTCAGAGTTAAAACTCACTTTAATAAGATGCAAAATCCCCCAAGCAGACTCTTGATAAAATTTTGCCCACTTCTAAGATACATCCTATGATGATTATAGGTTATAATAGATTGTTGTAAAACTGCTTTCCTGCAAAATGTGGGTGGAGGCTGGACCCATGACTCAAGATAGCGTAGTTCGAGTGTTCCCAACTAACTTGATAACTTGGGTGACAGCACTTGCTCCAGAATTACTATACCCGAGTAACAAGTGCACTTGTGCCAAATGGACTCATGCTTCCTGAAGAGTTCGGGAGATATTGATGGAATTATATGTGCTTCCCCTGAAACATTGGCATTTGACTTCTTTCTTATTGACTTCTTTCAGGAAAGGTGTACAGAGACATAGTAGGGAGTGCTTATTATGTTGCCCCTGAAGTATTACGTCGTAAATATGGGAAAGAAATTGATATTTGGAGTGCTGGAGTCATGTTGTACATATTACTCAGTGGCGTACCTCCATTTTGGGCAGGTAACAGAGAGGATTACATTCTTTTTCTCCTTGCTATGCTCCGTCTATCAAACATCCTATCACATTTTCTTTTTCTGAAAATTGCCTGGCTGGTATTGTGAATAACATAATCTTTATTGCAGAAACTGAGAAGGGCATATTTGATGCAATATTGCAAGGACATATTGACTTTGAAAGTGATCCATGGCCACATATTTCCAACAGTGCCAAGGACCTAGTGAAGAAAATGTTAACTCAGGACCCAGCAACCCGGATTACGTCGGCGCAGGTTCTCGGTATGTCTGTATCTCGTTTGTGAATGCTTTCTTGAATTCTTTGCCTAAATAAAATCTGCAAAAGATCGGTATATGAATATATATCTTCTAACTGGGGGTATGCGATTGTCTATCTTATTTAGAGCATCCGTGGATGAGAGAAGGCGGAGATGCATCGGATAAGCCAATAGACAGTGCAGTATTTACGAGACTCAAGCAATTTAGAGCAATGAACAAACTGAAGAAACTTGCCCTGAAGGTGATTGGTTATCTTTTCTACATAGCAGACGTTATACTTAATTGAGGGGCCGGGGATAAAGAGTTCTCTGTAACCGTAGTTGATTTATTTATTATTATTATTTTTTTGTTGGGGGGGGGGGGGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGTGTGAAGAAGTTCATGATATTAATAGGTAGTTCATGAAACAATTGTAGGTTATAGCTGAAAATCTTTCTGAGGAAGAGATCCAAGGCCTGAAAGCAATGTTCACAAACATGGACACCGACAAAAGCGGAACCATCACTTATGCAGAATTGAAGTCTGGATTGGCCCGGCTTGGCTCAACACTAAGTGAGGCTGAGGTCAAACAACTTATGGAAGCGGTACTGTATGATTAAACATTATCTATCATCCAAAGTTCCTTATTTGTTTTTCCTCACATGAACTGAGAGAACTTGGTTTGCCGTTTCAGGCCGATGTGGATGGAAATGGTTCAATTGATTACATCGAATTCATCACTGCTACGATGCATAGATACAAGCTAGAAAAAGAAGAGCATCTTTACAAAGCCTTTCAGCATTTTGACAAGGATAACAGTGGGTCAGTCTCTAATCCTATAATATATAAGCATTTATCTGCTTTTTGAAACAACTTATTTCAGTCTTTTTGAGCATTTGCTTATTGTTCTTTCAGTTATATCACAAGAGATGAGCTAAAAGCTGCAATGAAAGATTATGGAATGGGCGATGAAGAGACTATTAGAGAAATTATTTCTGAAGTCGATGCAGACAACGTAAGTTCGTTCATATTTACCGTTTTAAGTTCTACCTAATTTTCTCTGGTAAAATGCAACTAACTTCTGTTCGGTTTTCATAGGATGGAAGAATCAACTATGATGAGTTCTGTTCAATGATGAGAAGTGGAACCCAACAAGCTAAACTCTTTTAA

mRNA sequence

ATGTTTGAAAAGTTACAAGTTTTATCTTCGGTTGTTTGTGGTATTCATATTGTTGTAAAACTGCTTTCCTGCAAAATGTGGGTGGAGGCTGGACCCATGACTCAAGATAGCGTAGTTCGAGTGTTCCCAACTAACTTGATAACTTGGGTGACAGCACTTGCTCCAGAATTACTATACCCGAGAAAGGTGTACAGAGACATAGTAGGGAGTGCTTATTATGTTGCCCCTGAAGTATTACGTCGTAAATATGGGAAAGAAATTGATATTTGGAGTGCTGGAGTCATGTTGTACATATTACTCAGTGGCGTACCTCCATTTTGGGCAGAAACTGAGAAGGGCATATTTGATGCAATATTGCAAGGACATATTGACTTTGAAAGTGATCCATGGCCACATATTTCCAACAGTGCCAAGGACCTAGTGAAGAAAATGTTAACTCAGGACCCAGCAACCCGGATTACGTCGGCGCAGGTTCTCGAGCATCCGTGGATGAGAGAAGGCGGAGATGCATCGGATAAGCCAATAGACAGTGCAGTATTTACGAGACTCAAGCAATTTAGAGCAATGAACAAACTGAAGAAACTTGCCCTGAAGGTTATAGCTGAAAATCTTTCTGAGGAAGAGATCCAAGGCCTGAAAGCAATGTTCACAAACATGGACACCGACAAAAGCGGAACCATCACTTATGCAGAATTGAAGTCTGGATTGGCCCGGCTTGGCTCAACACTAAGTGAGGCTGAGGTCAAACAACTTATGGAAGCGGCCGATGTGGATGGAAATGGTTCAATTGATTACATCGAATTCATCACTGCTACGATGCATAGATACAAGCTAGAAAAAGAAGAGCATCTTTACAAAGCCTTTCAGCATTTTGACAAGGATAACAGTGGTTATATCACAAGAGATGAGCTAAAAGCTGCAATGAAAGATTATGGAATGGGCGATGAAGAGACTATTAGAGAAATTATTTCTGAAGTCGATGCAGACAACGATGGAAGAATCAACTATGATGAGTTCTGTTCAATGATGAGAAGTGGAACCCAACAAGCTAAACTCTTTTAA

Coding sequence (CDS)

ATGTTTGAAAAGTTACAAGTTTTATCTTCGGTTGTTTGTGGTATTCATATTGTTGTAAAACTGCTTTCCTGCAAAATGTGGGTGGAGGCTGGACCCATGACTCAAGATAGCGTAGTTCGAGTGTTCCCAACTAACTTGATAACTTGGGTGACAGCACTTGCTCCAGAATTACTATACCCGAGAAAGGTGTACAGAGACATAGTAGGGAGTGCTTATTATGTTGCCCCTGAAGTATTACGTCGTAAATATGGGAAAGAAATTGATATTTGGAGTGCTGGAGTCATGTTGTACATATTACTCAGTGGCGTACCTCCATTTTGGGCAGAAACTGAGAAGGGCATATTTGATGCAATATTGCAAGGACATATTGACTTTGAAAGTGATCCATGGCCACATATTTCCAACAGTGCCAAGGACCTAGTGAAGAAAATGTTAACTCAGGACCCAGCAACCCGGATTACGTCGGCGCAGGTTCTCGAGCATCCGTGGATGAGAGAAGGCGGAGATGCATCGGATAAGCCAATAGACAGTGCAGTATTTACGAGACTCAAGCAATTTAGAGCAATGAACAAACTGAAGAAACTTGCCCTGAAGGTTATAGCTGAAAATCTTTCTGAGGAAGAGATCCAAGGCCTGAAAGCAATGTTCACAAACATGGACACCGACAAAAGCGGAACCATCACTTATGCAGAATTGAAGTCTGGATTGGCCCGGCTTGGCTCAACACTAAGTGAGGCTGAGGTCAAACAACTTATGGAAGCGGCCGATGTGGATGGAAATGGTTCAATTGATTACATCGAATTCATCACTGCTACGATGCATAGATACAAGCTAGAAAAAGAAGAGCATCTTTACAAAGCCTTTCAGCATTTTGACAAGGATAACAGTGGTTATATCACAAGAGATGAGCTAAAAGCTGCAATGAAAGATTATGGAATGGGCGATGAAGAGACTATTAGAGAAATTATTTCTGAAGTCGATGCAGACAACGATGGAAGAATCAACTATGATGAGTTCTGTTCAATGATGAGAAGTGGAACCCAACAAGCTAAACTCTTTTAA

Protein sequence

MFEKLQVLSSVVCGIHIVVKLLSCKMWVEAGPMTQDSVVRVFPTNLITWVTALAPELLYPRKVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQGHIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFTRLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGSTLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITRDELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF
Homology
BLAST of Lsi05G005940.1 vs. ExPASy Swiss-Prot
Match: Q9C6P3 (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 2.7e-138
Identity = 237/293 (80.89%), Postives = 272/293 (92.83%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           +VY+DIVGSAYYVAPEVL+R+YGKEIDIWSAG++LYILLSGVPPFWAETEKGIFDAIL+G
Sbjct: 229 RVYKDIVGSAYYVAPEVLKRRYGKEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEG 288

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDFES PWP ISNSAKDLV++MLTQDP  RI++A+VL+HPW+REGG+ASDKPIDSAV +
Sbjct: 289 EIDFESQPWPSISNSAKDLVRRMLTQDPKRRISAAEVLKHPWLREGGEASDKPIDSAVLS 348

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAEN+  EEIQGLKAMF N+DTD SGTITY ELK GLA+LGS
Sbjct: 349 RMKQFRAMNKLKKLALKVIAENIDTEEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGS 408

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            L+EAEVKQLM+AADVDGNGSIDYIEFITATMHR++LE  E++YKAFQHFDKD SGYIT 
Sbjct: 409 RLTEAEVKQLMDAADVDGNGSIDYIEFITATMHRHRLESNENVYKAFQHFDKDGSGYITT 468

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGT-QQAKLF 354
           DEL+AA+K+YGMGD+ TI+EI+S+VDADNDGRINYDEFC+MMRSG  QQ +LF
Sbjct: 469 DELEAALKEYGMGDDATIKEILSDVDADNDGRINYDEFCAMMRSGNPQQPRLF 521

BLAST of Lsi05G005940.1 vs. ExPASy Swiss-Prot
Match: Q38868 (Calcium-dependent protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CPK9 PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.8e-137
Identity = 235/295 (79.66%), Postives = 272/295 (92.20%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRR+YGKE+DIWSAG++LYILLSGVPPFWAETEKGIFDAIL+G
Sbjct: 247 KVYRDIVGSAYYVAPEVLRRRYGKEVDIWSAGIILYILLSGVPPFWAETEKGIFDAILEG 306

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFES PWP IS+SAKDLV++MLT DP  RI++A VL+HPW+REGG+ASDKPIDSAV +
Sbjct: 307 HIDFESQPWPSISSSAKDLVRRMLTADPKRRISAADVLQHPWLREGGEASDKPIDSAVLS 366

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAEN+  EEIQGLKAMF N+DTD SGTITY ELK GLA+LGS
Sbjct: 367 RMKQFRAMNKLKKLALKVIAENIDTEEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGS 426

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            L+EAEVKQLM+AADVDGNGSIDYIEFITATMHR++LE  E+LYKAFQHFDKD+SGYIT 
Sbjct: 427 KLTEAEVKQLMDAADVDGNGSIDYIEFITATMHRHRLESNENLYKAFQHFDKDSSGYITI 486

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGT---QQAKLF 354
           DEL++A+K+YGMGD+ TI+E++S+VD+DNDGRINY+EFC+MMRSG    QQ +LF
Sbjct: 487 DELESALKEYGMGDDATIKEVLSDVDSDNDGRINYEEFCAMMRSGNPQQQQPRLF 541

BLAST of Lsi05G005940.1 vs. ExPASy Swiss-Prot
Match: P28582 (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 490.3 bits (1261), Expect = 1.8e-137
Identity = 240/292 (82.19%), Postives = 271/292 (92.81%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYR+IVGSAYYVAPEVLRR YGKEIDIWSAGV+LYILLSGVPPFWAE EKGIFDAIL+G
Sbjct: 237 KVYRNIVGSAYYVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDAILEG 296

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDFES+PWP +SNSAKDLV+KMLTQDP  RITSAQVL+HPWMREGG+ASDKPIDSAV +
Sbjct: 297 VIDFESEPWPSVSNSAKDLVRKMLTQDPRRRITSAQVLDHPWMREGGEASDKPIDSAVLS 356

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLK+LALKVIAE+LSEEEI+GLK+MF NMDTDKSGTITY ELKSGLARLGS
Sbjct: 357 RMKQFRAMNKLKQLALKVIAESLSEEEIKGLKSMFANMDTDKSGTITYEELKSGLARLGS 416

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            LSE EV+QLM+AADVDGNG+IDY+EFITATMHR+KLE  EH  +AFQ+FDKDNSG+IT+
Sbjct: 417 KLSEVEVQQLMDAADVDGNGTIDYLEFITATMHRHKLESYEH--QAFQYFDKDNSGFITK 476

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DEL++AMK+YGMGDE TI++IISEVD+DNDGRINYDEFC+MMR    + KLF
Sbjct: 477 DELESAMKEYGMGDEATIKDIISEVDSDNDGRINYDEFCAMMRRARNRRKLF 526

BLAST of Lsi05G005940.1 vs. ExPASy Swiss-Prot
Match: P53683 (Calcium-dependent protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK19 PE=2 SV=2)

HSP 1 Score: 483.0 bits (1242), Expect = 2.8e-135
Identity = 235/288 (81.60%), Postives = 266/288 (92.36%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           K+YRDIVGSAYYVAPEVLRR YGKEID+WSAGV+LYILLSGVPPFWAETEKGIFDAILQG
Sbjct: 241 KMYRDIVGSAYYVAPEVLRRNYGKEIDVWSAGVILYILLSGVPPFWAETEKGIFDAILQG 300

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDFES PWP IS SAKDLV+KMLTQDP  RITSAQVL+HPW+R+ G+ASDKPIDSAV +
Sbjct: 301 EIDFESQPWPSISESAKDLVRKMLTQDPKKRITSAQVLQHPWLRD-GEASDKPIDSAVLS 360

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKK+ALKVIA NL+EEEI+GLK MFTNMDTD SGTITY ELK+GLA+LGS
Sbjct: 361 RMKQFRAMNKLKKMALKVIASNLNEEEIKGLKQMFTNMDTDNSGTITYEELKAGLAKLGS 420

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            LSEAEVKQLMEAADVDGNGSIDY+EFITATMHR+KLE++EHL+KAFQ+FDKDNSG+ITR
Sbjct: 421 KLSEAEVKQLMEAADVDGNGSIDYVEFITATMHRHKLERDEHLFKAFQYFDKDNSGFITR 480

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQ 350
           DEL++A+ ++ MGD  TI++IISEVD DNDGRINY+EFC+MMR G  Q
Sbjct: 481 DELESALIEHEMGDTSTIKDIISEVDTDNDGRINYEEFCAMMRGGGMQ 527

BLAST of Lsi05G005940.1 vs. ExPASy Swiss-Prot
Match: Q9ZSA2 (Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=1 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 8.2e-135
Identity = 232/288 (80.56%), Postives = 266/288 (92.36%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRR YGKEIDIWSAGV+LYILLSGVPPFWAE EKGIFD +++G
Sbjct: 236 KVYRDIVGSAYYVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKG 295

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDF S+PWP IS SAKDLV+KMLT+DP  RIT+AQVLEHPW++ GG+A DKPIDSAV +
Sbjct: 296 EIDFVSEPWPSISESAKDLVRKMLTKDPKRRITAAQVLEHPWIK-GGEAPDKPIDSAVLS 355

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAE+LSEEEI+GLK MF N+DTDKSGTITY ELK+GL RLGS
Sbjct: 356 RMKQFRAMNKLKKLALKVIAESLSEEEIKGLKTMFANIDTDKSGTITYEELKTGLTRLGS 415

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            LSE EVKQLMEAADVDGNG+IDY EFI+ATMHRYKL+++EH+YKAFQHFDKDNSG+ITR
Sbjct: 416 RLSETEVKQLMEAADVDGNGTIDYYEFISATMHRYKLDRDEHVYKAFQHFDKDNSGHITR 475

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQ 350
           DEL++AMK+YGMGDE +I+E+ISEVD DNDGRIN++EFC+MMRSG+ Q
Sbjct: 476 DELESAMKEYGMGDEASIKEVISEVDTDNDGRINFEEFCAMMRSGSTQ 522

BLAST of Lsi05G005940.1 vs. ExPASy TrEMBL
Match: A0A0A0L2E3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G077600 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.6e-160
Identity = 285/292 (97.60%), Postives = 290/292 (99.32%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEID+WSAGVMLYILLSGVPPFWAETEKGIFD+ILQG
Sbjct: 228 KVYRDIVGSAYYVAPEVLRRKYGKEIDVWSAGVMLYILLSGVPPFWAETEKGIFDSILQG 287

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFESDPWPHIS SAKDLVK+MLTQDPA RITSAQVLEHPWMREGG+ASDKPIDSAVFT
Sbjct: 288 HIDFESDPWPHISPSAKDLVKRMLTQDPANRITSAQVLEHPWMREGGEASDKPIDSAVFT 347

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS
Sbjct: 348 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 407

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNG+IDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 408 TLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 467

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF
Sbjct: 468 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Lsi05G005940.1 vs. ExPASy TrEMBL
Match: A0A5A7SVI6 (Calcium-dependent protein kinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00220 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.4e-160
Identity = 286/292 (97.95%), Postives = 290/292 (99.32%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFD+ILQG
Sbjct: 228 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDSILQG 287

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFES+PWP IS+SAKDLVKKMLTQDPA RITSAQVLEHPWMREGGDASDKPIDSAVFT
Sbjct: 288 HIDFESEPWPQISHSAKDLVKKMLTQDPANRITSAQVLEHPWMREGGDASDKPIDSAVFT 347

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS
Sbjct: 348 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 407

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNG+IDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 408 TLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 467

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF
Sbjct: 468 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Lsi05G005940.1 vs. ExPASy TrEMBL
Match: A0A1S3CJN0 (calcium-dependent protein kinase-like OS=Cucumis melo OX=3656 GN=LOC103501584 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.4e-160
Identity = 286/292 (97.95%), Postives = 290/292 (99.32%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFD+ILQG
Sbjct: 228 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDSILQG 287

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFES+PWP IS+SAKDLVKKMLTQDPA RITSAQVLEHPWMREGGDASDKPIDSAVFT
Sbjct: 288 HIDFESEPWPQISHSAKDLVKKMLTQDPANRITSAQVLEHPWMREGGDASDKPIDSAVFT 347

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS
Sbjct: 348 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 407

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNG+IDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 408 TLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 467

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF
Sbjct: 468 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Lsi05G005940.1 vs. ExPASy TrEMBL
Match: A0A6J1F0W0 (calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111438480 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.4e-158
Identity = 281/292 (96.23%), Postives = 286/292 (97.95%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG
Sbjct: 227 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 286

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFE+DPWP ISNSAKDLVKKMLTQDPA RITSAQVL+HPWMREGGDASDKPIDSAVFT
Sbjct: 287 HIDFETDPWPKISNSAKDLVKKMLTQDPAQRITSAQVLDHPWMREGGDASDKPIDSAVFT 346

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLK  FTN+DTD SGTITYAELKSGLARLGS
Sbjct: 347 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKTTFTNLDTDNSGTITYAELKSGLARLGS 406

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 407 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 466

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFC+MMR+GTQQ KLF
Sbjct: 467 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCTMMRTGTQQGKLF 518

BLAST of Lsi05G005940.1 vs. ExPASy TrEMBL
Match: A0A6J1JAQ3 (calcium-dependent protein kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483276 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 6.1e-157
Identity = 280/292 (95.89%), Postives = 285/292 (97.60%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG
Sbjct: 227 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 286

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFE+DPWP ISNSAKDLVKKMLTQDPA RITSAQVL+HPWMREGGDASDKPIDSAVFT
Sbjct: 287 HIDFETDPWPKISNSAKDLVKKMLTQDPAQRITSAQVLDHPWMREGGDASDKPIDSAVFT 346

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLK  FTN+DTD SGTITYAELKSGLARLGS
Sbjct: 347 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKTTFTNLDTDNSGTITYAELKSGLARLGS 406

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 407 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 466

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDE TIREIISEVDADNDGRINYDEFC+MMR+GTQQ KLF
Sbjct: 467 DELKAAMKDYGMGDEGTIREIISEVDADNDGRINYDEFCTMMRTGTQQGKLF 518

BLAST of Lsi05G005940.1 vs. NCBI nr
Match: XP_004152931.1 (calcium-dependent protein kinase [Cucumis sativus] >KGN56125.1 hypothetical protein Csa_009506 [Cucumis sativus])

HSP 1 Score: 574.7 bits (1480), Expect = 5.4e-160
Identity = 285/292 (97.60%), Postives = 290/292 (99.32%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEID+WSAGVMLYILLSGVPPFWAETEKGIFD+ILQG
Sbjct: 228 KVYRDIVGSAYYVAPEVLRRKYGKEIDVWSAGVMLYILLSGVPPFWAETEKGIFDSILQG 287

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFESDPWPHIS SAKDLVK+MLTQDPA RITSAQVLEHPWMREGG+ASDKPIDSAVFT
Sbjct: 288 HIDFESDPWPHISPSAKDLVKRMLTQDPANRITSAQVLEHPWMREGGEASDKPIDSAVFT 347

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS
Sbjct: 348 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 407

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNG+IDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 408 TLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 467

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF
Sbjct: 468 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Lsi05G005940.1 vs. NCBI nr
Match: XP_038903170.1 (calcium-dependent protein kinase 2-like [Benincasa hispida])

HSP 1 Score: 574.7 bits (1480), Expect = 5.4e-160
Identity = 287/292 (98.29%), Postives = 288/292 (98.63%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG
Sbjct: 232 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 291

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFESDPWP IS SAKDLVKKMLTQDPA RITSAQVLEHPWMREGGDASDKPIDSAVFT
Sbjct: 292 HIDFESDPWPQISKSAKDLVKKMLTQDPANRITSAQVLEHPWMREGGDASDKPIDSAVFT 351

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS
Sbjct: 352 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 411

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNG+IDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 412 TLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 471

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAK F
Sbjct: 472 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKCF 523

BLAST of Lsi05G005940.1 vs. NCBI nr
Match: XP_008463413.1 (PREDICTED: calcium-dependent protein kinase-like [Cucumis melo] >KAA0033976.1 calcium-dependent protein kinase-like [Cucumis melo var. makuwa] >TYK02570.1 calcium-dependent protein kinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 574.3 bits (1479), Expect = 7.1e-160
Identity = 286/292 (97.95%), Postives = 290/292 (99.32%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFD+ILQG
Sbjct: 228 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDSILQG 287

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFES+PWP IS+SAKDLVKKMLTQDPA RITSAQVLEHPWMREGGDASDKPIDSAVFT
Sbjct: 288 HIDFESEPWPQISHSAKDLVKKMLTQDPANRITSAQVLEHPWMREGGDASDKPIDSAVFT 347

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS
Sbjct: 348 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 407

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNG+IDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 408 TLSEAEVKQLMEAADVDGNGTIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 467

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF
Sbjct: 468 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 519

BLAST of Lsi05G005940.1 vs. NCBI nr
Match: XP_023520424.1 (calcium-dependent protein kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.4 bits (1461), Expect = 8.7e-158
Identity = 282/292 (96.58%), Postives = 286/292 (97.95%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG
Sbjct: 227 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 286

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFE+DPWP ISNSAKDLVKKMLTQDPA RITSAQVL+HPWMREGGDASDKPIDSAVFT
Sbjct: 287 HIDFETDPWPKISNSAKDLVKKMLTQDPAQRITSAQVLDHPWMREGGDASDKPIDSAVFT 346

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLK  FTNMDTD SGTITYAELKSGLARLGS
Sbjct: 347 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKTTFTNMDTDNSGTITYAELKSGLARLGS 406

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 407 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 466

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFC+MMR+GTQQ KLF
Sbjct: 467 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCTMMRTGTQQGKLF 518

BLAST of Lsi05G005940.1 vs. NCBI nr
Match: XP_022932163.1 (calcium-dependent protein kinase 2-like [Cucurbita moschata])

HSP 1 Score: 566.2 bits (1458), Expect = 1.9e-157
Identity = 281/292 (96.23%), Postives = 286/292 (97.95%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG
Sbjct: 227 KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 286

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFE+DPWP ISNSAKDLVKKMLTQDPA RITSAQVL+HPWMREGGDASDKPIDSAVFT
Sbjct: 287 HIDFETDPWPKISNSAKDLVKKMLTQDPAQRITSAQVLDHPWMREGGDASDKPIDSAVFT 346

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           RLKQFRAMNKLKKLALKVIAENLSEEEIQGLK  FTN+DTD SGTITYAELKSGLARLGS
Sbjct: 347 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKTTFTNLDTDNSGTITYAELKSGLARLGS 406

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
           TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR
Sbjct: 407 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 466

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQAKLF 354
           DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFC+MMR+GTQQ KLF
Sbjct: 467 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCTMMRTGTQQGKLF 518

BLAST of Lsi05G005940.1 vs. TAIR 10
Match: AT1G50700.1 (calcium-dependent protein kinase 33 )

HSP 1 Score: 493.0 bits (1268), Expect = 1.9e-139
Identity = 237/293 (80.89%), Postives = 272/293 (92.83%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           +VY+DIVGSAYYVAPEVL+R+YGKEIDIWSAG++LYILLSGVPPFWAETEKGIFDAIL+G
Sbjct: 229 RVYKDIVGSAYYVAPEVLKRRYGKEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEG 288

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDFES PWP ISNSAKDLV++MLTQDP  RI++A+VL+HPW+REGG+ASDKPIDSAV +
Sbjct: 289 EIDFESQPWPSISNSAKDLVRRMLTQDPKRRISAAEVLKHPWLREGGEASDKPIDSAVLS 348

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAEN+  EEIQGLKAMF N+DTD SGTITY ELK GLA+LGS
Sbjct: 349 RMKQFRAMNKLKKLALKVIAENIDTEEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGS 408

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            L+EAEVKQLM+AADVDGNGSIDYIEFITATMHR++LE  E++YKAFQHFDKD SGYIT 
Sbjct: 409 RLTEAEVKQLMDAADVDGNGSIDYIEFITATMHRHRLESNENVYKAFQHFDKDGSGYITT 468

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGT-QQAKLF 354
           DEL+AA+K+YGMGD+ TI+EI+S+VDADNDGRINYDEFC+MMRSG  QQ +LF
Sbjct: 469 DELEAALKEYGMGDDATIKEILSDVDADNDGRINYDEFCAMMRSGNPQQPRLF 521

BLAST of Lsi05G005940.1 vs. TAIR 10
Match: AT3G20410.1 (calmodulin-domain protein kinase 9 )

HSP 1 Score: 490.3 bits (1261), Expect = 1.3e-138
Identity = 235/295 (79.66%), Postives = 272/295 (92.20%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRR+YGKE+DIWSAG++LYILLSGVPPFWAETEKGIFDAIL+G
Sbjct: 247 KVYRDIVGSAYYVAPEVLRRRYGKEVDIWSAGIILYILLSGVPPFWAETEKGIFDAILEG 306

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
           HIDFES PWP IS+SAKDLV++MLT DP  RI++A VL+HPW+REGG+ASDKPIDSAV +
Sbjct: 307 HIDFESQPWPSISSSAKDLVRRMLTADPKRRISAADVLQHPWLREGGEASDKPIDSAVLS 366

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAEN+  EEIQGLKAMF N+DTD SGTITY ELK GLA+LGS
Sbjct: 367 RMKQFRAMNKLKKLALKVIAENIDTEEIQGLKAMFANIDTDNSGTITYEELKEGLAKLGS 426

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            L+EAEVKQLM+AADVDGNGSIDYIEFITATMHR++LE  E+LYKAFQHFDKD+SGYIT 
Sbjct: 427 KLTEAEVKQLMDAADVDGNGSIDYIEFITATMHRHRLESNENLYKAFQHFDKDSSGYITI 486

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGT---QQAKLF 354
           DEL++A+K+YGMGD+ TI+E++S+VD+DNDGRINY+EFC+MMRSG    QQ +LF
Sbjct: 487 DELESALKEYGMGDDATIKEVLSDVDSDNDGRINYEEFCAMMRSGNPQQQQPRLF 541

BLAST of Lsi05G005940.1 vs. TAIR 10
Match: AT4G04720.1 (calcium-dependent protein kinase 21 )

HSP 1 Score: 481.5 bits (1238), Expect = 5.8e-136
Identity = 232/288 (80.56%), Postives = 266/288 (92.36%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRR YGKEIDIWSAGV+LYILLSGVPPFWAE EKGIFD +++G
Sbjct: 236 KVYRDIVGSAYYVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKG 295

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDF S+PWP IS SAKDLV+KMLT+DP  RIT+AQVLEHPW++ GG+A DKPIDSAV +
Sbjct: 296 EIDFVSEPWPSISESAKDLVRKMLTKDPKRRITAAQVLEHPWIK-GGEAPDKPIDSAVLS 355

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAE+LSEEEI+GLK MF N+DTDKSGTITY ELK+GL RLGS
Sbjct: 356 RMKQFRAMNKLKKLALKVIAESLSEEEIKGLKTMFANIDTDKSGTITYEELKTGLTRLGS 415

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            LSE EVKQLMEAADVDGNG+IDY EFI+ATMHRYKL+++EH+YKAFQHFDKDNSG+ITR
Sbjct: 416 RLSETEVKQLMEAADVDGNGTIDYYEFISATMHRYKLDRDEHVYKAFQHFDKDNSGHITR 475

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSGTQQ 350
           DEL++AMK+YGMGDE +I+E+ISEVD DNDGRIN++EFC+MMRSG+ Q
Sbjct: 476 DELESAMKEYGMGDEASIKEVISEVDTDNDGRINFEEFCAMMRSGSTQ 522

BLAST of Lsi05G005940.1 vs. TAIR 10
Match: AT4G21940.1 (calcium-dependent protein kinase 15 )

HSP 1 Score: 471.5 bits (1212), Expect = 6.1e-133
Identity = 227/294 (77.21%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRR YGKEIDIWSAG++LYILL GVPPFW+ETEKGIF+ I++G
Sbjct: 258 KVYRDIVGSAYYVAPEVLRRSYGKEIDIWSAGIILYILLCGVPPFWSETEKGIFNEIIKG 317

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDF+S PWP IS SAKDLV+K+LT+DP  RI++AQ LEHPW+R GG+A DKPIDSAV +
Sbjct: 318 EIDFDSQPWPSISESAKDLVRKLLTKDPKQRISAAQALEHPWIR-GGEAPDKPIDSAVLS 377

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAE+LSEEEI+GLK MF NMDTDKSGTITY ELK+GLA+LGS
Sbjct: 378 RMKQFRAMNKLKKLALKVIAESLSEEEIKGLKTMFANMDTDKSGTITYEELKNGLAKLGS 437

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            L+EAEVKQLMEAADVDGNG+IDYIEFI+ATMHRY+ +++EH++KAFQ+FDKDNSG+IT 
Sbjct: 438 KLTEAEVKQLMEAADVDGNGTIDYIEFISATMHRYRFDRDEHVFKAFQYFDKDNSGFITM 497

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSG---TQQAKL 353
           DEL++AMK+YGMGDE +I+E+I+EVD DNDGRINY+EFC+MMRSG    QQ K+
Sbjct: 498 DELESAMKEYGMGDEASIKEVIAEVDTDNDGRINYEEFCAMMRSGITLPQQGKI 550

BLAST of Lsi05G005940.1 vs. TAIR 10
Match: AT4G21940.2 (calcium-dependent protein kinase 15 )

HSP 1 Score: 471.5 bits (1212), Expect = 6.1e-133
Identity = 227/294 (77.21%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 62  KVYRDIVGSAYYVAPEVLRRKYGKEIDIWSAGVMLYILLSGVPPFWAETEKGIFDAILQG 121
           KVYRDIVGSAYYVAPEVLRR YGKEIDIWSAG++LYILL GVPPFW+ETEKGIF+ I++G
Sbjct: 258 KVYRDIVGSAYYVAPEVLRRSYGKEIDIWSAGIILYILLCGVPPFWSETEKGIFNEIIKG 317

Query: 122 HIDFESDPWPHISNSAKDLVKKMLTQDPATRITSAQVLEHPWMREGGDASDKPIDSAVFT 181
            IDF+S PWP IS SAKDLV+K+LT+DP  RI++AQ LEHPW+R GG+A DKPIDSAV +
Sbjct: 318 EIDFDSQPWPSISESAKDLVRKLLTKDPKQRISAAQALEHPWIR-GGEAPDKPIDSAVLS 377

Query: 182 RLKQFRAMNKLKKLALKVIAENLSEEEIQGLKAMFTNMDTDKSGTITYAELKSGLARLGS 241
           R+KQFRAMNKLKKLALKVIAE+LSEEEI+GLK MF NMDTDKSGTITY ELK+GLA+LGS
Sbjct: 378 RMKQFRAMNKLKKLALKVIAESLSEEEIKGLKTMFANMDTDKSGTITYEELKNGLAKLGS 437

Query: 242 TLSEAEVKQLMEAADVDGNGSIDYIEFITATMHRYKLEKEEHLYKAFQHFDKDNSGYITR 301
            L+EAEVKQLMEAADVDGNG+IDYIEFI+ATMHRY+ +++EH++KAFQ+FDKDNSG+IT 
Sbjct: 438 KLTEAEVKQLMEAADVDGNGTIDYIEFISATMHRYRFDRDEHVFKAFQYFDKDNSGFITM 497

Query: 302 DELKAAMKDYGMGDEETIREIISEVDADNDGRINYDEFCSMMRSG---TQQAKL 353
           DEL++AMK+YGMGDE +I+E+I+EVD DNDGRINY+EFC+MMRSG    QQ K+
Sbjct: 498 DELESAMKEYGMGDEASIKEVIAEVDTDNDGRINYEEFCAMMRSGITLPQQGKI 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6P32.7e-13880.89Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=... [more]
Q388681.8e-13779.66Calcium-dependent protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CPK9 PE=1 ... [more]
P285821.8e-13782.19Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2[more]
P536832.8e-13581.60Calcium-dependent protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q9ZSA28.2e-13580.56Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0L2E32.6e-16097.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G077600 PE=4 SV=1[more]
A0A5A7SVI63.4e-16097.95Calcium-dependent protein kinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CJN03.4e-16097.95calcium-dependent protein kinase-like OS=Cucumis melo OX=3656 GN=LOC103501584 PE... [more]
A0A6J1F0W09.4e-15896.23calcium-dependent protein kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JAQ36.1e-15795.89calcium-dependent protein kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111483... [more]
Match NameE-valueIdentityDescription
XP_004152931.15.4e-16097.60calcium-dependent protein kinase [Cucumis sativus] >KGN56125.1 hypothetical prot... [more]
XP_038903170.15.4e-16098.29calcium-dependent protein kinase 2-like [Benincasa hispida][more]
XP_008463413.17.1e-16097.95PREDICTED: calcium-dependent protein kinase-like [Cucumis melo] >KAA0033976.1 ca... [more]
XP_023520424.18.7e-15896.58calcium-dependent protein kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022932163.11.9e-15796.23calcium-dependent protein kinase 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G50700.11.9e-13980.89calcium-dependent protein kinase 33 [more]
AT3G20410.11.3e-13879.66calmodulin-domain protein kinase 9 [more]
AT4G04720.15.8e-13680.56calcium-dependent protein kinase 21 [more]
AT4G21940.16.1e-13377.21calcium-dependent protein kinase 15 [more]
AT4G21940.26.1e-13377.21calcium-dependent protein kinase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 2..164
e-value: 9.9E-10
score: 4.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 62..164
e-value: 2.6E-25
score: 89.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..164
score: 19.495745
IPR002048EF-hand domainSMARTSM00054efh_1coord: 283..311
e-value: 1.1E-5
score: 35.0
coord: 211..239
e-value: 3.1E-4
score: 30.1
coord: 318..346
e-value: 4.3E-7
score: 39.6
coord: 247..275
e-value: 0.14
score: 21.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 281..344
e-value: 5.7E-16
score: 58.7
coord: 212..272
e-value: 9.9E-11
score: 41.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 243..278
score: 11.054376
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 207..242
score: 13.843916
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 279..314
score: 16.828724
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 315..349
score: 12.7281
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 48..168
e-value: 3.5E-34
score: 119.9
NoneNo IPR availableGENE3D1.10.238.10coord: 171..349
e-value: 3.1E-61
score: 208.1
NoneNo IPR availablePANTHERPTHR24349:SF386CALCIUM-DEPENDENT PROTEIN KINASE 21-LIKEcoord: 62..349
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 62..349
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 256..268
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 220..232
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 327..339
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 292..304
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 62..186
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 203..344

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi05G005940Lsi05G005940gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi05G005940.1.exon.1Lsi05G005940.1.exon.1exon
Lsi05G005940.1.exon.2Lsi05G005940.1.exon.2exon
Lsi05G005940.1.exon.3Lsi05G005940.1.exon.3exon
Lsi05G005940.1.exon.4Lsi05G005940.1.exon.4exon
Lsi05G005940.1.exon.5Lsi05G005940.1.exon.5exon
Lsi05G005940.1.exon.6Lsi05G005940.1.exon.6exon
Lsi05G005940.1.exon.7Lsi05G005940.1.exon.7exon
Lsi05G005940.1.exon.8Lsi05G005940.1.exon.8exon
Lsi05G005940.1.exon.9Lsi05G005940.1.exon.9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi05G005940.1.CDS.1Lsi05G005940.1.CDS.1CDS
Lsi05G005940.1.CDS.2Lsi05G005940.1.CDS.2CDS
Lsi05G005940.1.CDS.3Lsi05G005940.1.CDS.3CDS
Lsi05G005940.1.CDS.4Lsi05G005940.1.CDS.4CDS
Lsi05G005940.1.CDS.5Lsi05G005940.1.CDS.5CDS
Lsi05G005940.1.CDS.6Lsi05G005940.1.CDS.6CDS
Lsi05G005940.1.CDS.7Lsi05G005940.1.CDS.7CDS
Lsi05G005940.1.CDS.8Lsi05G005940.1.CDS.8CDS
Lsi05G005940.1.CDS.9Lsi05G005940.1.CDS.9CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi05G005940.1Lsi05G005940.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity