Lsi02G013280.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G013280.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr02: 17769215 .. 17773175 (-)
Sequence length1278
RNA-Seq ExpressionLsi02G013280.1
SyntenyLsi02G013280.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAAACGGAAGAAGCAGTTCCACGCCGCCTGGCGTTTCCATTCTCCTCCACGGCTCAGCCATCCGAGGCGGCACCACGTCAAAGGTATTTTTTTTCTTCTCATTCCTACTGTTCTTCGTGAAGAATCTTCTCATCCCACTGTGAACTCGGGCGGCAATAATCCGATGGCAATTCGGTGGTTTTCCAGGTCGAAATTGCCCGTTTTTGCGTCGGTATTTGTACGATGTCTAACGAGGAGACCAATTAGTGATATCCCATTACCAGCGAAATCGACTATTACTGGACTTCAGACGGATTCGAGCCGACCCTTATGGGTATTCCGGCTATATCACGACGGGAGGCCGCGGGGACCCCTTTGGAGAAGCCGGAAAGCAATTGGGAAAGAAGCACTTTTCGTTATTCAGGGATTGAAGCGATTCAAGGAAGATGAAGAGAAACTCGAGAAGTTCATGAAGAGCCATGTTTTGAGGCTCTTGAAATTGGATATGATTGCTGTGCTTGGTGAACTTGAGCGGCAGGAAGAGGTTGCTTTAGCTGTTAAGGTTTGTTCCCCCTTCCTTTTCTTCTATATGTTCTGTCCTTTTTGTAGCTCTTTCTGAATCTTGCCATACATTTTAGATTTTACTTCATGGGTTGTCTCAGAAATAGTTAATGGGCTTCTGTAAATTACACATAATATCGCTATCTTGCTCTTTTTTGTTGTCACTGCAACTTCTTTACCTAGTTAGTTGATGCTGTGAGTTCTTTTTTCCTTCTTAACTGTTATGTTATTGATTGTTATTTACTTTAGGTGAATAACTTTGCTAATGCTGCATTCAAGCTTCTATTCTGCTCTTGAATTTCTAAGATTATGTTTCTCAGCTTCTAGCTTTCTTAGTATGAATATGATGTTCTTGACTTTATGTCTCCTTTCATGGAGCTATTGTGCAATTTTTTTCAAGTTTCAGCTCTTCCTCTCTTTATGCATGGTAGTAATTGAAGCATGCTTAAGGAAGGTCATAGAGGAAGTAAATTCATGTATTGGTGTTCCTTTAAATGATATTATGAGTTTTTTTTTTCCCACTTCCTCTTATATGTTTAGGCTTGATTTATGTCGAGGATGTTGCCTTTGCATTCAAGATTCTTTCTTCCATATGAATACTGCTTGTTTCTCTCTTGTTCAACTCTGATCTGCCCCTTTGAGTGCTTATCAGATCTAGTTCATACTTTTTGTATGAATTAAGATCTCAAATATGAAATCTAGTTCACTCTGGTTGCATGTGGAACGTTTGCTTTTATATTCTTTTATCTTCAACTCGGGATTGAATTGATTTTATTTCATCGTGTCTGTGAATCTTACATGTTGAATTTTCTAGATATTTAGGTTGATTCGAAAACAAGACTGGTACACACCTGATGTTTTTATATATAAAGACTTGATTATTGCATTAGCTAGAAGTAAAAAGATGGATGACGCAATGAAATTATGGGAAAGCATGAGAGAGGAGAATTTATTCCCCGACTCTCAAACGTATACTGAAGTCATCAGAGGTTTCTTGAGATATGGATCTCCTTCTGATGCAATGAACGTATATGAGGATATGAAGAAGTCTCCAGATCCACCAGATGAGTTGCCATTTAGAATTTTGTTGAAAGGCCTTTTGCCACACCCCCTTTTGAGAAACAGAGTAAAGCAAGATTTTGAGGAGCTCTTTCCTGACCAGCATGTGTACGATCCCCCAGAAGAGATATTCGGCCTACGTTGAGGTATCTATGCAACTCTTTTTTCCTCTTTAAATCTTTCTTTTCCATTAACGTTGACATTTGTTGTATTTTGAAGGATGGTAGTACTTAGTAAGCAGTTATAGTAGGCCATTCGCCATTGTATGGTTTATAAATTCAAAATCCTTAAGTTACTTGTGACAGTTAACATCTTTCAAGTTTTTAAATACCAAATAGTTTTCACTTTTTTGAAGTAATTGGAAAATGGCCAAAACGTGCCTGTGAAAGACAGTAAAATTCGCTTGACGAGCACAAAGGAGGTAGGATAAAGATTTGATCAGATATAGAGCGAGTCATGTGGATAGTAGTTGAGTTGGAGCCTAAAACTCTCTTAGTCTTAGGGTTCTCTCGTCTTGGATACTTGGGAAGAGGATCAAGTGAGTCATGTGGTCCTTGTAAACAACTTGATGCACTATCTTCTTTCAGCCCTTTAAATGAAATGCACCAAAACTTTAAAGGGAGGAAAATCCATAGATCAACCACCCCATCATCTCTGCTCTGTAAAAACTGTAAACAGCAAGATCACCCTTTGGATGTCTTCAATATTTAGGGGTTACGAACCAACCATAGAGTCCTATTCAACAGGTGAGGGACTTATCCTCCACCCTCCCTTTCGTGATAAAATTTTGGTTAATATAATATATTACACATATTAATAAAAAAAATTGGTTTTATTAGGATGATAAACATAGCATTGTGCTCATTCAATTTTAAAAACAAATTAAAGTACTGAATCCTAATGAAATTAATAATTAAAATTGTAAAATAATATTTGTACATAGAATGTGGATAGTTGATTTTATTTTTAATGGCAAGCAACGGTGATAGTCGGTTCGGTTTTATCAGTTTTGGAGCCAACCAAGAACTAAACCAAACCAATGGTTTTACAAAGAAAGATCCAAACTAATGTTCAATAAAGAATAAAACCAATGGATTGGTTTTTATTTGGTTCAATTTATTATTGGTTTGGTTCTTAGTTTTTGGTATTTTTATTTTTTTCTTTCAAATTTTTATTTTTATTTCCACCTTTGTTTTGAATTGGTTATTTTTGCTAATTTTAAATTAAAATTGGGTAAATTTCTTTTTTTTATAGATTGAAATTTGATGTTTTTGTTTTTTATTGGCACTTTTTTAAATAAAAAATATACAAACCCCATAATAGTTGGTCTTTTTTTTCTTCTAAACAACAAATATACATGCACCATAATGATTAAAATACTAAAAGGTACCTAATGTTAGAGTTTATTTTTAAGGGTATATATATACCATTGGTTCGGTTTGGTTTTGATTGGTTTTTTTAAATAAAAAATCGATACAAACCAATACATCCAATTTTATACTAAAAAAATCAAACTAAACTATTGGATTGATTTGAATTGGTTTGGTTTTTCGATTTTCGGTAATTGCAAGGGGTAAAAGATGGAGAAGAATAATGAGAATATGAGATAGTAACCAAGTGGGGATTGGGAACAAAAAGCAAAAGCATCTTTCCTCAAATCTCATATCCCCACCAATAAAGAAATCCCTAGTGATCCCAAATTAAATAATTTTTATTTCAATTGGAGAAGCCTTTTGGATATTTATTGAGTGGCTCATTTGGTCAAAAAAAAAAAAAAAAAAAACCAAACAAACAAACATATGCTCATTCTCTAGGAAGATATTTGATTCTCATTTTTCATAAGTTTTGGGTTCTTCTTGATAATTAAGTTGGATGAATTAACGATATATTTGAATGGTATAAGAATAAGTTCCATTAACCTTCCTGTAAAAAGGATCATGAGAATAATAAATTTAATCAATTGGTTAAAGTATTATAATTTCTGTCATGATATAAATAAAAATTATTTCAAATCAGGAAAGTGCGAGGAAGAATATTTTTAACCCAAATCTAGTCCTGCTTTCGTCGATGATGATGGTTGACGACTAGACGATAAGATTGCTAAAGGAGGGGTTGAAGGAAGAAGAGGTAAGAGAAAGATGAGGAGGATCATGTGAAGGAGAGCAAATAAGTAAACTTTTTTTTTTAAAAAAAAATTATACTATTATTATTATTATTATTATTTTAAAAGTTTACACATTAGCGTCGAGTTAACGAAGCTGTTAATCGAGTGGTCGAATTGAATTATATCATAAACTTTGAAAACTTGATTGTACGACTTTAGAACTTAATGGTATGTTTGGAACCTCATGATTTTGATA

mRNA sequence

CTAAAACGGAAGAAGCAGTTCCACGCCGCCTGGCGTTTCCATTCTCCTCCACGGCTCAGCCATCCGAGGCGGCACCACGTCAAAGGTATTTTTTTTCTTCTCATTCCTACTGTTCTTCGTGAAGAATCTTCTCATCCCACTGTGAACTCGGGCGGCAATAATCCGATGGCAATTCGGTGGTTTTCCAGGTCGAAATTGCCCGTTTTTGCGTCGGTATTTGTACGATGTCTAACGAGGAGACCAATTAGTGATATCCCATTACCAGCGAAATCGACTATTACTGGACTTCAGACGGATTCGAGCCGACCCTTATGGGTATTCCGGCTATATCACGACGGGAGGCCGCGGGGACCCCTTTGGAGAAGCCGGAAAGCAATTGGGAAAGAAGCACTTTTCGTTATTCAGGGATTGAAGCGATTCAAGGAAGATGAAGAGAAACTCGAGAAGTTCATGAAGAGCCATGTTTTGAGGCTCTTGAAATTGGATATGATTGCTGTGCTTGGTGAACTTGAGCGGCAGGAAGAGGTTGCTTTAGCTGTTAAGATATTTAGGTTGATTCGAAAACAAGACTGGTACACACCTGATGTTTTTATATATAAAGACTTGATTATTGCATTAGCTAGAAGTAAAAAGATGGATGACGCAATGAAATTATGGGAAAGCATGAGAGAGGAGAATTTATTCCCCGACTCTCAAACGTATACTGAAGTCATCAGAGGTTTCTTGAGATATGGATCTCCTTCTGATGCAATGAACGTATATGAGGATATGAAGAAGTCTCCAGATCCACCAGATGAGTTGCCATTTAGAATTTTGTTGAAAGGCCTTTTGCCACACCCCCTTTTGAGAAACAGAGTAAAGCAAGATTTTGAGGAGCTCTTTCCTGACCAGCATGTGTACGATCCCCCAGAAGAGATATTCGGCCTACGTTGAGGAAAGTGCGAGGAAGAATATTTTTAACCCAAATCTAGTCCTGCTTTCGTCGATGATGATGGTTGACGACTAGACGATAAGATTGCTAAAGGAGGGGTTGAAGGAAGAAGAGGTAAGAGAAAGATGAGGAGGATCATGTGAAGGAGAGCAAATAAGTAAACTTTTTTTTTTAAAAAAAAATTATACTATTATTATTATTATTATTATTTTAAAAGTTTACACATTAGCGTCGAGTTAACGAAGCTGTTAATCGAGTGGTCGAATTGAATTATATCATAAACTTTGAAAACTTGATTGTACGACTTTAGAACTTAATGGTATGTTTGGAACCTCATGATTTTGATA

Coding sequence (CDS)

CTAAAACGGAAGAAGCAGTTCCACGCCGCCTGGCGTTTCCATTCTCCTCCACGGCTCAGCCATCCGAGGCGGCACCACGTCAAAGGTATTTTTTTTCTTCTCATTCCTACTGTTCTTCGTGAAGAATCTTCTCATCCCACTGTGAACTCGGGCGGCAATAATCCGATGGCAATTCGGTGGTTTTCCAGGTCGAAATTGCCCGTTTTTGCGTCGGTATTTGTACGATGTCTAACGAGGAGACCAATTAGTGATATCCCATTACCAGCGAAATCGACTATTACTGGACTTCAGACGGATTCGAGCCGACCCTTATGGGTATTCCGGCTATATCACGACGGGAGGCCGCGGGGACCCCTTTGGAGAAGCCGGAAAGCAATTGGGAAAGAAGCACTTTTCGTTATTCAGGGATTGAAGCGATTCAAGGAAGATGAAGAGAAACTCGAGAAGTTCATGAAGAGCCATGTTTTGAGGCTCTTGAAATTGGATATGATTGCTGTGCTTGGTGAACTTGAGCGGCAGGAAGAGGTTGCTTTAGCTGTTAAGATATTTAGGTTGATTCGAAAACAAGACTGGTACACACCTGATGTTTTTATATATAAAGACTTGATTATTGCATTAGCTAGAAGTAAAAAGATGGATGACGCAATGAAATTATGGGAAAGCATGAGAGAGGAGAATTTATTCCCCGACTCTCAAACGTATACTGAAGTCATCAGAGGTTTCTTGAGATATGGATCTCCTTCTGATGCAATGAACGTATATGAGGATATGAAGAAGTCTCCAGATCCACCAGATGAGTTGCCATTTAGAATTTTGTTGAAAGGCCTTTTGCCACACCCCCTTTTGAGAAACAGAGTAAAGCAAGATTTTGAGGAGCTCTTTCCTGACCAGCATGTGTACGATCCCCCAGAAGAGATATTCGGCCTACGTTGA

Protein sequence

LKRKKQFHAAWRFHSPPRLSHPRRHHVKGIFFLLIPTVLREESSHPTVNSGGNNPMAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRPRGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEEVALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYTEVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFPDQHVYDPPEEIFGLR
Homology
BLAST of Lsi02G013280.1 vs. ExPASy Swiss-Prot
Match: Q9STF9 (Protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THA8L PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 5.4e-98
Identity = 178/254 (70.08%), Postives = 210/254 (82.68%), Query Frame = 0

Query: 57  AIRWFSRSKLPVFASVFVRCLTRRP-ISDIPLPAKSTITGLQTDSSRPLWVF-RLYHDGR 116
           AIR  SR K P FAS+F + +TR P I  I        T L     +P  VF   +HDGR
Sbjct: 3   AIRVCSR-KFPTFASIFFQNITRNPSIHRISFSNLKPKTLLHPIPPKPFTVFVSRFHDGR 62

Query: 117 PRGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQE 176
           PRGPLWR +K IGKEALFVI GLKR KED+EKL+KF+K+HV RLLKLDM+AV+GELERQE
Sbjct: 63  PRGPLWRGKKLIGKEALFVILGLKRLKEDDEKLDKFIKTHVFRLLKLDMLAVIGELERQE 122

Query: 177 EVALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTY 236
           E ALA+K+F +I+KQ+WY PDVF+YKDLI++LA+SK+MD+AM LWE M++ENLFPDSQTY
Sbjct: 123 ETALAIKMFEVIQKQEWYQPDVFMYKDLIVSLAKSKRMDEAMALWEKMKKENLFPDSQTY 182

Query: 237 TEVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELF 296
           TEVIRGFLR G P+DAMNVYEDM KSPDPP+ELPFR+LLKGLLPHPLLRN+VK+DFEELF
Sbjct: 183 TEVIRGFLRDGCPADAMNVYEDMLKSPDPPEELPFRVLLKGLLPHPLLRNKVKKDFEELF 242

Query: 297 PDQHVYDPPEEIFG 309
           P++H YDPPEEIFG
Sbjct: 243 PEKHAYDPPEEIFG 255

BLAST of Lsi02G013280.1 vs. ExPASy Swiss-Prot
Match: Q1PFH7 (Pentatricopeptide repeat-containing protein At1g62350 OS=Arabidopsis thaliana OX=3702 GN=At1g62350 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-46
Identity = 84/181 (46.41%), Postives = 131/181 (72.38%), Query Frame = 0

Query: 126 IGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEEVALAVKIFRL 185
           + KE L   + LKR +    +L++F+ SHV RLLK D+++VL E +RQ +V L +K++ +
Sbjct: 1   MSKEGLIAAKELKRLQTQSVRLDRFIGSHVSRLLKSDLVSVLAEFQRQNQVFLCMKLYEV 60

Query: 186 IRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYTEVIRGFLRYG 245
           +R++ WY PD+F Y+D+++ LAR+KK+D+  K+WE +++E +  D  T+ +++RGFL   
Sbjct: 61  VRREIWYRPDMFFYRDMLMMLARNKKVDETKKVWEDLKKEEVLFDQHTFGDLVRGFLDNE 120

Query: 246 SPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFPDQHVYDPPEE 305
            P +AM +Y +M++SPD P  LPFR++LKGL+P+P LR +VK DF ELFP   VYDPPE+
Sbjct: 121 LPLEAMRLYGEMRESPDRPLSLPFRVILKGLVPYPELREKVKDDFLELFPGMIVYDPPED 180

Query: 306 I 307
           I
Sbjct: 181 I 181

BLAST of Lsi02G013280.1 vs. ExPASy Swiss-Prot
Match: Q9FMQ1 (Pentatricopeptide repeat-containing protein At5g12100, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g12100 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.1e-09
Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = 0

Query: 175 EVALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTY 234
           +V   +++F  + K D   P VFIY  LI  L + K+M+DA +L++ M    L P   TY
Sbjct: 194 DVGKGLELFNRM-KHDRIYPSVFIYNVLIDGLCKGKRMNDAEQLFDEMLARRLLPSLITY 253

Query: 235 TEVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGL 277
             +I G+ + G+P  +  V E MK     P  + F  LLKGL
Sbjct: 254 NTLIDGYCKAGNPEKSFKVRERMKADHIEPSLITFNTLLKGL 294

BLAST of Lsi02G013280.1 vs. ExPASy Swiss-Prot
Match: Q9FKC3 (Pentatricopeptide repeat-containing protein At5g48730, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g48730 PE=2 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-08
Identity = 45/161 (27.95%), Postives = 80/161 (49.69%), Query Frame = 0

Query: 121 RSRKAIGKEALFVI---QGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEEVA 180
           ++ K   ++A+ +I   +  K   E ++  +K +   VL  L   + A+  E        
Sbjct: 80  KNTKIASRKAISIILRREATKSIIEKKKGSKKLLPRTVLESLHERITALRWE-------- 139

Query: 181 LAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYTEV 240
            A+++F L+R+Q WY P+V IY  LI+ L + K+ + A +L++ M  E    + + YT +
Sbjct: 140 SAIQVFELLREQLWYKPNVGIYVKLIVMLGKCKQPEKAHELFQEMINEGCVVNHEVYTAL 199

Query: 241 IRGFLRYGSPSDAMNVYEDMKKSPD-PPDELPFRILLKGLL 278
           +  + R G    A  + E MK S +  PD   + IL+K  L
Sbjct: 200 VSAYSRSGRFDAAFTLLERMKSSHNCQPDVHTYSILIKSFL 232

BLAST of Lsi02G013280.1 vs. ExPASy Swiss-Prot
Match: Q9LUR2 (Putative pentatricopeptide repeat-containing protein At3g16710, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g16710 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.0e-08
Identity = 32/98 (32.65%), Postives = 54/98 (55.10%), Query Frame = 0

Query: 179 AVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYTEVI 238
           A ++F L+ +   Y P+  IY  LI    +SK+++D MK++  M ++ +  ++ TYT +I
Sbjct: 312 ARQMFYLMERNGCY-PNEVIYTTLIHGFCKSKRVEDGMKIFYEMSQKGVVANTITYTVLI 371

Query: 239 RGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGL 277
           +G+   G P  A  V+  M     PPD   + +LL GL
Sbjct: 372 QGYCLVGRPDVAQEVFNQMSSRRAPPDIRTYNVLLDGL 408

BLAST of Lsi02G013280.1 vs. ExPASy TrEMBL
Match: A0A0A0LVE9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525290 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 9.2e-133
Identity = 236/255 (92.55%), Postives = 243/255 (95.29%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MAIRWFSRSKLPVFASVF++ LTRRPI D+PLP KSTIT  Q DS RP+W FRLYHDGRP
Sbjct: 1   MAIRWFSRSKLPVFASVFLQGLTRRPIRDVPLPVKSTITDFQPDSRRPIWGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEK EKFMKSHV RLLKLDM+AVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKFEKFMKSHVSRLLKLDMVAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVKIFRLIRKQDWY PDV+IYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHV+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

BLAST of Lsi02G013280.1 vs. ExPASy TrEMBL
Match: A0A5A7SRJ7 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00030 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.5e-132
Identity = 235/255 (92.16%), Postives = 243/255 (95.29%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MAIRWFSRSKLP+FA VF+R LTRRPI D+PLP  STITG Q DSSRP+W FRLYHDGRP
Sbjct: 1   MAIRWFSRSKLPIFALVFLRGLTRRPIRDVPLPVTSTITGFQPDSSRPIWGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL KFMKSHV RLLKLDM+AVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLGKFMKSHVSRLLKLDMLAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVKIFRLIRKQDWY PDV+IYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFLRYGSPSDAMNVYEDMKKSP+PPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPEPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHV+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

BLAST of Lsi02G013280.1 vs. ExPASy TrEMBL
Match: A0A1S3C5H5 (pentatricopeptide repeat-containing protein At3g46870 OS=Cucumis melo OX=3656 GN=LOC103497124 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.5e-132
Identity = 235/255 (92.16%), Postives = 243/255 (95.29%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MAIRWFSRSKLP+FA VF+R LTRRPI D+PLP  STITG Q DSSRP+W FRLYHDGRP
Sbjct: 1   MAIRWFSRSKLPIFALVFLRGLTRRPIRDVPLPVTSTITGFQPDSSRPIWGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL KFMKSHV RLLKLDM+AVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLGKFMKSHVSRLLKLDMLAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVKIFRLIRKQDWY PDV+IYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFLRYGSPSDAMNVYEDMKKSP+PPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPEPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHV+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

BLAST of Lsi02G013280.1 vs. ExPASy TrEMBL
Match: A0A6J1I496 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469502 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.4e-127
Identity = 227/255 (89.02%), Postives = 241/255 (94.51%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MA+RWFS+SKLP+  SVF+R LTRRPI DIPLP KSTI G   DSSR L  FRLYHDGRP
Sbjct: 1   MAMRWFSKSKLPICYSVFLRGLTRRPIRDIPLPVKSTIAGFLPDSSRLLSGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL+KFMK HVLRLLKLDMIAVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKCHVLRLLKLDMIAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVK+FRLI+KQ+W+ PDVFIYKDLI+ALARSKKMDDAM+LWESMR+ENLFPDSQTYT
Sbjct: 121 VALAVKVFRLIQKQEWFKPDVFIYKDLIVALARSKKMDDAMELWESMRKENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFL+YGSPSDAMN+YEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLKYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHVYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

BLAST of Lsi02G013280.1 vs. ExPASy TrEMBL
Match: A0A6J1HLK1 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464704 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.4e-127
Identity = 224/255 (87.84%), Postives = 241/255 (94.51%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MA+RWFS+ KLP+  SVF+R L RRPI DIPLP KST+ G Q DSSRPL  FRLYHDGRP
Sbjct: 1   MAMRWFSKLKLPICYSVFLRGLMRRPIRDIPLPVKSTVAGFQPDSSRPLSGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL+KFMK HVLRLLKLDMIAVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKCHVLRLLKLDMIAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVK+FRLI+KQ+WY PDVF+YKDLI+ALARSK+MDDAM+LWESMR+ENLFPDSQTYT
Sbjct: 121 VALAVKVFRLIQKQEWYKPDVFLYKDLIVALARSKQMDDAMELWESMRKENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFL+YGSPSDAMN+YEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEE+FP
Sbjct: 181 EVIRGFLKYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEEIFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHVYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

BLAST of Lsi02G013280.1 vs. NCBI nr
Match: XP_038895593.1 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida] >XP_038895594.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida] >XP_038895595.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida])

HSP 1 Score: 490.7 bits (1262), Expect = 9.1e-135
Identity = 241/255 (94.51%), Postives = 246/255 (96.47%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MAIRWFSRSKLP+FASVF+R LTRRPI D  LP KSTITG Q DSSRPLW FRLYHDGRP
Sbjct: 1   MAIRWFSRSKLPIFASVFLRGLTRRPIRDSSLPVKSTITGFQPDSSRPLWGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMI+VLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMISVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVKIFRLI+KQDWY PDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT
Sbjct: 121 VALAVKIFRLIQKQDWYKPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFLRYGSPSDAMN+YEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHVYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

BLAST of Lsi02G013280.1 vs. NCBI nr
Match: XP_008457435.1 (PREDICTED: pentatricopeptide repeat-containing protein At3g46870 [Cucumis melo] >KAA0031765.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 481.1 bits (1237), Expect = 7.2e-132
Identity = 235/255 (92.16%), Postives = 243/255 (95.29%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MAIRWFSRSKLP+FA VF+R LTRRPI D+PLP  STITG Q DSSRP+W FRLYHDGRP
Sbjct: 1   MAIRWFSRSKLPIFALVFLRGLTRRPIRDVPLPVTSTITGFQPDSSRPIWGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL KFMKSHV RLLKLDM+AVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLGKFMKSHVSRLLKLDMLAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVKIFRLIRKQDWY PDV+IYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFLRYGSPSDAMNVYEDMKKSP+PPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPEPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHV+DPPEEIF LR
Sbjct: 241 DQHVFDPPEEIFSLR 255

BLAST of Lsi02G013280.1 vs. NCBI nr
Match: KAE8653270.1 (hypothetical protein Csa_023162 [Cucumis sativus])

HSP 1 Score: 468.8 bits (1205), Expect = 3.7e-128
Identity = 229/247 (92.71%), Postives = 236/247 (95.55%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MAIRWFSRSKLPVFASVF++ LTRRPI D+PLP KSTIT  Q DS RP+W FRLYHDGRP
Sbjct: 1   MAIRWFSRSKLPVFASVFLQGLTRRPIRDVPLPVKSTITDFQPDSRRPIWGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEK EKFMKSHV RLLKLDM+AVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKFEKFMKSHVSRLLKLDMVAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVKIFRLIRKQDWY PDV+IYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDP 303
           DQHV+DP
Sbjct: 241 DQHVFDP 247

BLAST of Lsi02G013280.1 vs. NCBI nr
Match: XP_004145279.2 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucumis sativus] >XP_031741152.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucumis sativus] >KAE8653263.1 hypothetical protein Csa_020110 [Cucumis sativus])

HSP 1 Score: 468.8 bits (1205), Expect = 3.7e-128
Identity = 229/247 (92.71%), Postives = 236/247 (95.55%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MAIRWFSRSKLPVFASVF++ LTRRPI D+PLP KSTIT  Q DS RP+W FRLYHDGRP
Sbjct: 1   MAIRWFSRSKLPVFASVFLQGLTRRPIRDVPLPVKSTITDFQPDSRRPIWGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEK EKFMKSHV RLLKLDM+AVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKFEKFMKSHVSRLLKLDMVAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVKIFRLIRKQDWY PDV+IYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT
Sbjct: 121 VALAVKIFRLIRKQDWYKPDVYIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP
Sbjct: 181 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 240

Query: 296 DQHVYDP 303
           DQHV+DP
Sbjct: 241 DQHVFDP 247

BLAST of Lsi02G013280.1 vs. NCBI nr
Match: XP_022964699.1 (protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >XP_022964700.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >XP_022964701.1 protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >KAG7019169.1 Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 464.5 bits (1194), Expect = 7.0e-127
Identity = 224/255 (87.84%), Postives = 241/255 (94.51%), Query Frame = 0

Query: 56  MAIRWFSRSKLPVFASVFVRCLTRRPISDIPLPAKSTITGLQTDSSRPLWVFRLYHDGRP 115
           MA+RWFS+ KLP+  SVF+R L RRPI DIPLP KST+ G Q DSSRPL  FRLYHDGRP
Sbjct: 1   MAMRWFSKLKLPICYSVFLRGLMRRPIRDIPLPVKSTVAGFQPDSSRPLSGFRLYHDGRP 60

Query: 116 RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEE 175
           RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKL+KFMK HVLRLLKLDMIAVLGELERQEE
Sbjct: 61  RGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLQKFMKCHVLRLLKLDMIAVLGELERQEE 120

Query: 176 VALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYT 235
           VALAVK+FRLI+KQ+WY PDVF+YKDLI+ALARSK+MDDAM+LWESMR+ENLFPDSQTYT
Sbjct: 121 VALAVKVFRLIQKQEWYKPDVFLYKDLIVALARSKQMDDAMELWESMRKENLFPDSQTYT 180

Query: 236 EVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFP 295
           EVIRGFL+YGSPSDAMN+YEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEE+FP
Sbjct: 181 EVIRGFLKYGSPSDAMNIYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEEIFP 240

Query: 296 DQHVYDPPEEIFGLR 311
           DQHVYDPPEEIFGLR
Sbjct: 241 DQHVYDPPEEIFGLR 255

BLAST of Lsi02G013280.1 vs. TAIR 10
Match: AT3G46870.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 3.8e-99
Identity = 178/254 (70.08%), Postives = 210/254 (82.68%), Query Frame = 0

Query: 57  AIRWFSRSKLPVFASVFVRCLTRRP-ISDIPLPAKSTITGLQTDSSRPLWVF-RLYHDGR 116
           AIR  SR K P FAS+F + +TR P I  I        T L     +P  VF   +HDGR
Sbjct: 3   AIRVCSR-KFPTFASIFFQNITRNPSIHRISFSNLKPKTLLHPIPPKPFTVFVSRFHDGR 62

Query: 117 PRGPLWRSRKAIGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQE 176
           PRGPLWR +K IGKEALFVI GLKR KED+EKL+KF+K+HV RLLKLDM+AV+GELERQE
Sbjct: 63  PRGPLWRGKKLIGKEALFVILGLKRLKEDDEKLDKFIKTHVFRLLKLDMLAVIGELERQE 122

Query: 177 EVALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTY 236
           E ALA+K+F +I+KQ+WY PDVF+YKDLI++LA+SK+MD+AM LWE M++ENLFPDSQTY
Sbjct: 123 ETALAIKMFEVIQKQEWYQPDVFMYKDLIVSLAKSKRMDEAMALWEKMKKENLFPDSQTY 182

Query: 237 TEVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELF 296
           TEVIRGFLR G P+DAMNVYEDM KSPDPP+ELPFR+LLKGLLPHPLLRN+VK+DFEELF
Sbjct: 183 TEVIRGFLRDGCPADAMNVYEDMLKSPDPPEELPFRVLLKGLLPHPLLRNKVKKDFEELF 242

Query: 297 PDQHVYDPPEEIFG 309
           P++H YDPPEEIFG
Sbjct: 243 PEKHAYDPPEEIFG 255

BLAST of Lsi02G013280.1 vs. TAIR 10
Match: AT1G62350.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 188.3 bits (477), Expect = 9.0e-48
Identity = 84/181 (46.41%), Postives = 131/181 (72.38%), Query Frame = 0

Query: 126 IGKEALFVIQGLKRFKEDEEKLEKFMKSHVLRLLKLDMIAVLGELERQEEVALAVKIFRL 185
           + KE L   + LKR +    +L++F+ SHV RLLK D+++VL E +RQ +V L +K++ +
Sbjct: 1   MSKEGLIAAKELKRLQTQSVRLDRFIGSHVSRLLKSDLVSVLAEFQRQNQVFLCMKLYEV 60

Query: 186 IRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTYTEVIRGFLRYG 245
           +R++ WY PD+F Y+D+++ LAR+KK+D+  K+WE +++E +  D  T+ +++RGFL   
Sbjct: 61  VRREIWYRPDMFFYRDMLMMLARNKKVDETKKVWEDLKKEEVLFDQHTFGDLVRGFLDNE 120

Query: 246 SPSDAMNVYEDMKKSPDPPDELPFRILLKGLLPHPLLRNRVKQDFEELFPDQHVYDPPEE 305
            P +AM +Y +M++SPD P  LPFR++LKGL+P+P LR +VK DF ELFP   VYDPPE+
Sbjct: 121 LPLEAMRLYGEMRESPDRPLSLPFRVILKGLVPYPELREKVKDDFLELFPGMIVYDPPED 180

Query: 306 I 307
           I
Sbjct: 181 I 181

BLAST of Lsi02G013280.1 vs. TAIR 10
Match: AT5G09320.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-16
Identity = 59/187 (31.55%), Postives = 93/187 (49.73%), Query Frame = 0

Query: 106 VFRLYHDGRPRGPLWRSRKAIGKEALFVIQGLKR---------------FKEDEEKLEKF 165
           V ++    + R PL R R  +  EA+  +Q LKR                      L++ 
Sbjct: 486 VIKMRDRSKNRKPLQRGR-MLSIEAIQAVQALKRANPLLPPPPVPSTSTTSSSSALLDRV 545

Query: 166 MKSHVLRLLKLDMIAVLGELERQEEVALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSK 225
           + S   RLLK DM+AVL EL RQ E +LA+K+F  IRK+ WY P V +Y D+I  +A + 
Sbjct: 546 IISKFRRLLKFDMVAVLRELLRQNECSLALKVFEEIRKEYWYKPQVRMYTDMITVMADNS 605

Query: 226 KMDDAMKLWESMR-EENLFPDSQTYTEVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPF 277
            M++   L+ +M+ E+ L  + + +  ++   L +      M+ Y  M+     PD   F
Sbjct: 606 LMEEVNYLYSAMKSEKGLMAEIEWFNTLLTILLNHKLFDLVMDCYAFMQSIGYEPDRASF 665

BLAST of Lsi02G013280.1 vs. TAIR 10
Match: AT5G12100.1 (pentatricopeptide (PPR) repeat-containing protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-10
Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = 0

Query: 175 EVALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDSQTY 234
           +V   +++F  + K D   P VFIY  LI  L + K+M+DA +L++ M    L P   TY
Sbjct: 194 DVGKGLELFNRM-KHDRIYPSVFIYNVLIDGLCKGKRMNDAEQLFDEMLARRLLPSLITY 253

Query: 235 TEVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLKGL 277
             +I G+ + G+P  +  V E MK     P  + F  LLKGL
Sbjct: 254 NTLIDGYCKAGNPEKSFKVRERMKADHIEPSLITFNTLLKGL 294

BLAST of Lsi02G013280.1 vs. TAIR 10
Match: AT1G77340.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 4.4e-10
Identity = 33/103 (32.04%), Postives = 61/103 (59.22%), Query Frame = 0

Query: 172 RQEEVALAVKIFRLIRKQDWYTPDVFIYKDLIIALARSKKMDDAMKLWESMREENLFPDS 231
           R++EV  A K+F+ + +   +  +V  Y  LI  L  +KK+D+A+ L   M+++N  P+ 
Sbjct: 202 RRKEVDAAFKVFKEMTQNGCHRNEV-SYTQLIYGLFEAKKIDEALSLLVKMKDDNCCPNV 261

Query: 232 QTYTEVIRGFLRYGSPSDAMNVYEDMKKSPDPPDELPFRILLK 275
           +TYT +I      G  S+AMN+++ M +S   PD+  + +L++
Sbjct: 262 RTYTVLIDALCGSGQKSEAMNLFKQMSESGIKPDDCMYTVLIQ 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9STF95.4e-9870.08Protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q1PFH71.3e-4646.41Pentatricopeptide repeat-containing protein At1g62350 OS=Arabidopsis thaliana OX... [more]
Q9FMQ12.1e-0936.27Pentatricopeptide repeat-containing protein At5g12100, mitochondrial OS=Arabidop... [more]
Q9FKC31.4e-0827.95Pentatricopeptide repeat-containing protein At5g48730, chloroplastic OS=Arabidop... [more]
Q9LUR23.0e-0832.65Putative pentatricopeptide repeat-containing protein At3g16710, mitochondrial OS... [more]
Match NameE-valueIdentityDescription
A0A0A0LVE99.2e-13392.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525290 PE=4 SV=1[more]
A0A5A7SRJ73.5e-13292.16Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C5H53.5e-13292.16pentatricopeptide repeat-containing protein At3g46870 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1I4963.4e-12789.02protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1HLK13.4e-12787.84protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
XP_038895593.19.1e-13594.51protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Benincasa hispida] >XP_0388955... [more]
XP_008457435.17.2e-13292.16PREDICTED: pentatricopeptide repeat-containing protein At3g46870 [Cucumis melo] ... [more]
KAE8653270.13.7e-12892.71hypothetical protein Csa_023162 [Cucumis sativus][more]
XP_004145279.23.7e-12892.71protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucumis sativus] >XP_031741152... [more]
XP_022964699.17.0e-12787.84protein THYLAKOID ASSEMBLY 8-like, chloroplastic [Cucurbita moschata] >XP_022964... [more]
Match NameE-valueIdentityDescription
AT3G46870.13.8e-9970.08Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G62350.19.0e-4846.41Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G09320.11.4e-1631.55Vacuolar sorting protein 9 (VPS9) domain [more]
AT5G12100.11.5e-1036.27pentatricopeptide (PPR) repeat-containing protein [more]
AT1G77340.14.4e-1032.04Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 194..242
e-value: 3.5E-10
score: 39.9
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 233..260
e-value: 1.2E-4
score: 20.0
coord: 199..230
e-value: 5.1E-6
score: 24.3
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 195..229
score: 11.114816
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 230..264
score: 10.599635
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 124..310
e-value: 7.2E-56
score: 190.5
NoneNo IPR availablePANTHERPTHR46870:SF2PROTEIN THYLAKOID ASSEMBLY 8-LIKE, CHLOROPLASTICcoord: 57..309
IPR044795Pentatricopeptide repeat-containing protein THA8L-likePANTHERPTHR46870PROTEIN THYLAKOID ASSEMBLY 8-LIKE, CHLOROPLASTICcoord: 57..309

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi02G013280Lsi02G013280gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi02G013280.1.exon.3Lsi02G013280.1.exon.3exon
Lsi02G013280.1.exon.2Lsi02G013280.1.exon.2exon
Lsi02G013280.1.exon.1Lsi02G013280.1.exon.1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi02G013280.1.three_prime_UTR.2Lsi02G013280.1.three_prime_UTR.2three_prime_UTR
Lsi02G013280.1.three_prime_UTR.1Lsi02G013280.1.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi02G013280.1.CDS.2Lsi02G013280.1.CDS.2CDS
Lsi02G013280.1.CDS.1Lsi02G013280.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi02G013280.1Lsi02G013280.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding