Lcy10g002080.1 (mRNA) Sponge gourd (P93075) v1

Overview
NameLcy10g002080.1
TypemRNA
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr10: 2693649 .. 2694038 (-)
Sequence length390
RNA-Seq ExpressionLcy10g002080.1
SyntenyLcy10g002080.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCAGCAGAGTGTTACAGGGAAATAAAATGTTCAGTTGAATGGGCAGAAGCAAAGGCGCTTATTGATGGTGTCCAACTAGCTCTTGATGCAGGCATACAACCAATTTGGGTAGAAACAGATTCACAGATAGTTTGGAATCTTCTTAACGATCGAGATCAACACTTCAATGAGATTGCTCTTCTCATTCAACACCTAAAGCAATCACAACTCTCCCATCATATTCGGAGTTTTCTGTTAACGCCAAGGGAGGGAAATAGAGTTGCACATACACTAGCCGTTCACGCTCGCACCACCAAACATAACGAGGTTTGGTTAGAAGATTGTCCTCCATGGGCTCATGGCATTCTTGCTCAAGAGATGCCAACCACAGTGGTCACACATTAG

mRNA sequence

ATGGCAGCAGCAGAGTGTTACAGGGAAATAAAATGTTCAGTTGAATGGGCAGAAGCAAAGGCGCTTATTGATGGTGTCCAACTAGCTCTTGATGCAGGCATACAACCAATTTGGGTAGAAACAGATTCACAGATAGTTTGGAATCTTCTTAACGATCGAGATCAACACTTCAATGAGATTGCTCTTCTCATTCAACACCTAAAGCAATCACAACTCTCCCATCATATTCGGAGTTTTCTGTTAACGCCAAGGGAGGGAAATAGAGTTGCACATACACTAGCCGTTCACGCTCGCACCACCAAACATAACGAGGTTTGGTTAGAAGATTGTCCTCCATGGGCTCATGGCATTCTTGCTCAAGAGATGCCAACCACAGTGGTCACACATTAG

Coding sequence (CDS)

ATGGCAGCAGCAGAGTGTTACAGGGAAATAAAATGTTCAGTTGAATGGGCAGAAGCAAAGGCGCTTATTGATGGTGTCCAACTAGCTCTTGATGCAGGCATACAACCAATTTGGGTAGAAACAGATTCACAGATAGTTTGGAATCTTCTTAACGATCGAGATCAACACTTCAATGAGATTGCTCTTCTCATTCAACACCTAAAGCAATCACAACTCTCCCATCATATTCGGAGTTTTCTGTTAACGCCAAGGGAGGGAAATAGAGTTGCACATACACTAGCCGTTCACGCTCGCACCACCAAACATAACGAGGTTTGGTTAGAAGATTGTCCTCCATGGGCTCATGGCATTCTTGCTCAAGAGATGCCAACCACAGTGGTCACACATTAG

Protein sequence

MAAAECYREIKCSVEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIALLIQHLKQSQLSHHIRSFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPPWAHGILAQEMPTTVVTH
Homology
BLAST of Lcy10g002080.1 vs. ExPASy TrEMBL
Match: A0A5C7H0P0 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 7.7e-09
Identity = 38/114 (33.33%), Postives = 63/114 (55.26%), Query Frame = 0

Query: 13  SVEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIALLIQHLKQSQL 72
           SVE  EA+A ++G+ LA+D G+  + +E+D+  V  LL+D+     E+  +I        
Sbjct: 322 SVEMLEAQACLEGIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGA 381

Query: 73  SHHIRSFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPPWAHGILAQEMPTTV 127
           S ++ S++   RE N VAH +A HA +     VW E+ PP    ++  + PT+V
Sbjct: 382 SVNLLSYVAVRREANSVAHCIAQHALSLDSPVVWFEEMPPDIARLVRGDSPTSV 435

BLAST of Lcy10g002080.1 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-08
Identity = 39/111 (35.14%), Postives = 58/111 (52.25%), Query Frame = 0

Query: 1   MAAAECYREIKCSVEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEI 60
           MA+A  Y E   SV+ AEA   ++G+QLA   G+ P+ +ETDS  ++NL +   +  +E 
Sbjct: 71  MASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSET 130

Query: 61  ALLIQHLKQSQLSHHIRSFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCP 112
             ++   K         SF    REGN+ AH LA  A   +   +W+ED P
Sbjct: 131 GEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMEDWP 181

BLAST of Lcy10g002080.1 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.9e-08
Identity = 40/107 (37.38%), Postives = 59/107 (55.14%), Query Frame = 0

Query: 14  VEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIALLIQHLKQSQLS 73
           V+W E  A+ +G+ LA++AG     +ETDS  ++NLL       +E+ +L   +K    S
Sbjct: 154 VDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSS 213

Query: 74  HHIR-SFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPPWAHGILA 120
           H  R SF  T R GN  AH LA  A T+ H ++W+E+ P     +LA
Sbjct: 214 HAERVSFSFTHRNGNAXAHLLAQLALTSPHLQIWVEEWPDEISSVLA 260

BLAST of Lcy10g002080.1 vs. ExPASy TrEMBL
Match: A0A5C7IIT4 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_004125 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.5e-07
Identity = 40/132 (30.30%), Postives = 67/132 (50.76%), Query Frame = 0

Query: 5   ECYREIKCSV----------EWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRD 64
           +CY  +  S+          +  EA A++ G +LAL+AG+ P  +E+DS  V NL+N  D
Sbjct: 733 DCYGHVMASLCQSFPGLLQPQTVEAVAVLRGFRLALEAGLCPASIESDSLSVVNLINSMD 792

Query: 65  QHFNEIALLIQHLKQSQLSHHIRSFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPPWA 124
               EI +++  +     +    S    PR  N VAH+LA  + + +   VWLEDCP   
Sbjct: 793 ILRAEIGVVLHDILAMGSNSFFSSVSFVPRLTNCVAHSLAKLSLSFEGEHVWLEDCPLAV 852

Query: 125 HGILAQEMPTTV 127
             ++  + P+++
Sbjct: 853 ESLVLGDCPSSL 864

BLAST of Lcy10g002080.1 vs. ExPASy TrEMBL
Match: A0A2N9EU90 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.2e-07
Identity = 41/111 (36.94%), Postives = 59/111 (53.15%), Query Frame = 0

Query: 3   AAECYR-EIKCSVEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIA 62
           A+ C+R     SVE  EA A    +Q+A+D G+Q I +E DSQ V + L +    F    
Sbjct: 547 ASLCHRIHYPHSVEAMEAYAARSAIQMAIDLGLQAIDIEGDSQTVVDALLNHAPCFTLYG 606

Query: 63  LLIQHLKQSQLSHHIRSFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPP 113
            LI     +  S H   FL   R+GN +AH++A  A+ ++  EVW+E  PP
Sbjct: 607 NLINDTNIATQSCHFVQFLHVKRDGNSIAHSIAKRAKFSQPFEVWMESVPP 657

BLAST of Lcy10g002080.1 vs. NCBI nr
Match: KAG6608327.1 (hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 85.9 bits (211), Expect = 2.8e-13
Identity = 39/81 (48.15%), Postives = 55/81 (67.90%), Query Frame = 0

Query: 25  GVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIALLIQHLKQSQLSHHIRSFLLTPR 84
           G QL +  G+  +W + +S++VW LL D+DQHF EIA LI+HLK    +H I+ FL TP+
Sbjct: 63  GNQLGMATGLTSVWPKVNSKVVWTLLMDQDQHFIEIAHLIKHLKHLSQNHCIKGFLPTPK 122

Query: 85  EGNRVAHTLAVHARTTKHNEV 106
           +  +  HTLA+HA  TKH E+
Sbjct: 123 DDTKATHTLAIHAWHTKHLEI 143

BLAST of Lcy10g002080.1 vs. NCBI nr
Match: TXG50387.1 (hypothetical protein EZV62_022911 [Acer yangbiense])

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-08
Identity = 38/114 (33.33%), Postives = 63/114 (55.26%), Query Frame = 0

Query: 13  SVEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIALLIQHLKQSQL 72
           SVE  EA+A ++G+ LA+D G+  + +E+D+  V  LL+D+     E+  +I        
Sbjct: 322 SVEMLEAQACLEGIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGA 381

Query: 73  SHHIRSFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPPWAHGILAQEMPTTV 127
           S ++ S++   RE N VAH +A HA +     VW E+ PP    ++  + PT+V
Sbjct: 382 SVNLLSYVAVRREANSVAHCIAQHALSLDSPVVWFEEMPPDIARLVRGDSPTSV 435

BLAST of Lcy10g002080.1 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 68.9 bits (167), Expect = 3.6e-08
Identity = 39/111 (35.14%), Postives = 58/111 (52.25%), Query Frame = 0

Query: 1   MAAAECYREIKCSVEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEI 60
           MA+A  Y E   SV+ AEA   ++G+QLA   G+ P+ +ETDS  ++NL +   +  +E 
Sbjct: 71  MASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSET 130

Query: 61  ALLIQHLKQSQLSHHIRSFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCP 112
             ++   K         SF    REGN+ AH LA  A   +   +W+ED P
Sbjct: 131 GEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMEDWP 181

BLAST of Lcy10g002080.1 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 68.2 bits (165), Expect = 6.1e-08
Identity = 40/107 (37.38%), Postives = 59/107 (55.14%), Query Frame = 0

Query: 14  VEWAEAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIALLIQHLKQSQLS 73
           V+W E  A+ +G+ LA++AG     +ETDS  ++NLL       +E+ +L   +K    S
Sbjct: 154 VDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIKLFLSS 213

Query: 74  HHIR-SFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPPWAHGILA 120
           H  R SF  T R GN  AH LA  A T+ H ++W+E+ P     +LA
Sbjct: 214 HAERVSFSFTHRNGNAXAHLLAQLALTSPHLQIWVEEWPDEISSVLA 260

BLAST of Lcy10g002080.1 vs. NCBI nr
Match: KAF8391778.1 (hypothetical protein HHK36_022012 [Tetracentron sinense])

HSP 1 Score: 67.8 bits (164), Expect = 7.9e-08
Identity = 38/105 (36.19%), Postives = 63/105 (60.00%), Query Frame = 0

Query: 18  EAKALIDGVQLALDAGIQPIWVETDSQIVWNLLNDRDQHFNEIALLIQHLKQSQLSHHIR 77
           EA A  +G++ AL+ GIQ + +E+DS    + L  ++++ +  +L++  +K  +LS H  
Sbjct: 871 EAIAAREGLKFALELGIQEVILESDSINTIHALTSQEENSSFESLVLDDVKTLRLSFHHC 930

Query: 78  SFLLTPREGNRVAHTLAVHARTTKHNEVWLEDCPPWAHGILAQEM 123
           SF    REGN VAH LA  A +   + VW+ED PP+   +L Q++
Sbjct: 931 SFSHVHREGNSVAHCLARKALSIVDHIVWMEDAPPFQTNVLLQDV 975

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5C7H0P07.7e-0933.33Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1[more]
A0A6J1CIF11.7e-0835.14uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1DBJ72.9e-0837.38uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A5C7IIT42.5e-0730.30Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_004125 PE=4 SV=1[more]
A0A2N9EU904.2e-0736.94Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAG6608327.12.8e-1348.15hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sorori... [more]
TXG50387.11.6e-0833.33hypothetical protein EZV62_022911 [Acer yangbiense][more]
XP_022140628.13.6e-0835.14uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_022150944.16.1e-0837.38uncharacterized protein LOC111018973 [Momordica charantia][more]
KAF8391778.17.9e-0836.19hypothetical protein HHK36_022012 [Tetracentron sinense][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 12..96
e-value: 1.9E-19
score: 69.7
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..108
e-value: 2.6E-11
score: 45.5
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 3..122
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 12..94
e-value: 4.29282E-18
score: 72.3468
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 11..100

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lcy10g002080Lcy10g002080gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy10g002080.1.exon1Lcy10g002080.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy10g002080.1.cds1Lcy10g002080.1.cds1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lcy10g002080.1Lcy10g002080.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity