Lcy10g001220.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy10g001220.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	18.5 kDa class I heat shock protein-like
Location	Chr10: 1429422 .. 1430406 (+)
Sequence length	985
RNA-Seq Expression	Lcy10g001220.1
Synteny	Lcy10g001220.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTACCAGACATCATGTATTCCCAGGCATCTATAAAACCTTAAACCAAACGGGCCCGGGGTTAAAAATGACAATTTCCCTTCGTTATCCATTTCACAAGGAATCTTAGAAGGTCCTAGAAGAAGCTGGAATCCGTTCCTTCTTGAATCAGTCCTTTCAATTTCATCTCCACTCTACTTCCCTTCAATTCCAAATCCCATCCTCGCCGGAGCTTTCAGAACTTCACCGACGATGTCGATCATTCCAATCGGCGGCCGAGACGGCCGTATTTCCAACACCTCCTCTGCAAACATCCTCAATCACTTCCCAAATTTCCCCTTCCCTCTTGACCTATGGCACGATTTCCCTTTCCCTTTCCCTTCCTCCATCTCCCGCGCCTTCCCGGACTTCGCCTTTGGAGGCTCCGTCAACACTCGCCTCGACTGGACCGAGACGCCGAACGCTCATGTCCTCAGAGCCTCCCTTCCCGGATTCGACGGTGATGACGTTCTCGTTGAGCTTCAGGATGATCGGATGCTCCAGATCAGCACCGAGAGCGGCAGCTTCCTTAGCAGATTCAAGATTCCGGAGAGTGGAAAGATCGAGGAGTTGAGCGCGTTTATGGATTTTGGAGTTCTCACTGTGTTTGTTCCAAAAGAAGATGATCGGAGCAGACGCGATATCCGAGTGGTGGAGATCACCGGCGAGTAGCTAGAGGTCGTCCTTTATACAAGTAAGAATCTAATTCTGAATGCTCTGTTTAGGATGAGAATCTGTTTGCGGTGAACTGGTGATGCGTGCTTAGTTTGAACTCTTCAAGTCGGTTTGCTCACAATGTGTTCGATGAAATGCTTCTGTGGTAATGTAATCTTGTGGTTCTCTATGCTATCTTAGAATGTAAACTGTTCAAATACGTCTGTTAATAAAATTTTAATTTGGTGCCTCTATGCAAAAAAGCGTTCTATGTTAGTTTAAATCTTGTTTTGCTCTTCAAACTTAGAACATAT

mRNA sequence

ATTACCAGACATCATGTATTCCCAGGCATCTATAAAACCTTAAACCAAACGGGCCCGGGGTTAAAAATGACAATTTCCCTTCGTTATCCATTTCACAAGGAATCTTAGAAGGTCCTAGAAGAAGCTGGAATCCGTTCCTTCTTGAATCAGTCCTTTCAATTTCATCTCCACTCTACTTCCCTTCAATTCCAAATCCCATCCTCGCCGGAGCTTTCAGAACTTCACCGACGATGTCGATCATTCCAATCGGCGGCCGAGACGGCCGTATTTCCAACACCTCCTCTGCAAACATCCTCAATCACTTCCCAAATTTCCCCTTCCCTCTTGACCTATGGCACGATTTCCCTTTCCCTTTCCCTTCCTCCATCTCCCGCGCCTTCCCGGACTTCGCCTTTGGAGGCTCCGTCAACACTCGCCTCGACTGGACCGAGACGCCGAACGCTCATGTCCTCAGAGCCTCCCTTCCCGGATTCGACGGTGATGACGTTCTCGTTGAGCTTCAGGATGATCGGATGCTCCAGATCAGCACCGAGAGCGGCAGCTTCCTTAGCAGATTCAAGATTCCGGAGAGTGGAAAGATCGAGGAGTTGAGCGCGTTTATGGATTTTGGAGTTCTCACTGTGTTTGTTCCAAAAGAAGATGATCGGAGCAGACGCGATATCCGAGTGGTGGAGATCACCGGCGAGTAGCTAGAGGTCGTCCTTTATACAAGTAAGAATCTAATTCTGAATGCTCTGTTTAGGATGAGAATCTGTTTGCGGTGAACTGGTGATGCGTGCTTAGTTTGAACTCTTCAAGTCGGTTTGCTCACAATGTGTTCGATGAAATGCTTCTGTGGTAATGTAATCTTGTGGTTCTCTATGCTATCTTAGAATGTAAACTGTTCAAATACGTCTGTTAATAAAATTTTAATTTGGTGCCTCTATGCAAAAAAGCGTTCTATGTTAGTTTAAATCTTGTTTTGCTCTTCAAACTTAGAACATAT

Coding sequence (CDS)

ATGTCGATCATTCCAATCGGCGGCCGAGACGGCCGTATTTCCAACACCTCCTCTGCAAACATCCTCAATCACTTCCCAAATTTCCCCTTCCCTCTTGACCTATGGCACGATTTCCCTTTCCCTTTCCCTTCCTCCATCTCCCGCGCCTTCCCGGACTTCGCCTTTGGAGGCTCCGTCAACACTCGCCTCGACTGGACCGAGACGCCGAACGCTCATGTCCTCAGAGCCTCCCTTCCCGGATTCGACGGTGATGACGTTCTCGTTGAGCTTCAGGATGATCGGATGCTCCAGATCAGCACCGAGAGCGGCAGCTTCCTTAGCAGATTCAAGATTCCGGAGAGTGGAAAGATCGAGGAGTTGAGCGCGTTTATGGATTTTGGAGTTCTCACTGTGTTTGTTCCAAAAGAAGATGATCGGAGCAGACGCGATATCCGAGTGGTGGAGATCACCGGCGAGTAG

Protein sequence

MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDRSRRDIRVVEITGE

Homology

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BLAST of Lcy10g001220.1 vs. ExPASy Swiss-Prot
Match: P05478 (18.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP18.5-C PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.7e-26
Identity = 70/175 (40.00%), Postives = 105/175 (60.00%), Query Frame = 0


Query: 1   MSIIP--IGGRDGRISNTSSANILNHFPNFPFPLDLWHDF-PFPFPSSISRA-FPDFAFG 60
           MS+IP   GGR   + +             PF LD+W  F  FPFP+++S A FP+F+  
Sbjct: 1   MSLIPNFFGGRRNNVFD-------------PFSLDVWDPFKDFPFPNTLSSASFPEFSRE 60

Query: 61  GS--VNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTE------------- 120
            S  V+TR+DW ETP AHV +A +PG   ++V V+++DD++LQIS E             
Sbjct: 61  NSAFVSTRVDWKETPEAHVFKADIPGLKKEEVKVQIEDDKVLQISGERNVEKEDKNDTWH 120

Query: 121 -----SGSFLSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDRSRRDIRVVEITG 152
                SG F+ RF++PE+ K+E++ A M+ GVLTV VPKE+ + + D++ +EI+G
Sbjct: 121 RVERSSGKFMRRFRLPENAKVEQVKASMENGVLTVTVPKEEVK-KPDVKAIEISG 161

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy Swiss-Prot
Match: P04793 (17.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.5-M PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-24
Identity = 68/172 (39.53%), Postives = 98/172 (56.98%), Query Frame = 0


Query: 1   MSIIP--IGGRDGRISNTSSANILNHFPNFPFPLDLWHDF-PFPFPSSISRAFPDFAFGG 60
           MS+IP   GGR   + +             PF LD+W  F  F FP+S+S     F    
Sbjct: 1   MSLIPSIFGGRRSNVFD-------------PFSLDVWDPFKDFHFPTSLSAENSAF---- 60

Query: 61  SVNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTE---------------- 120
            VNTR+DW ETP AHV  A +PG   ++V V+++DDR+LQIS E                
Sbjct: 61  -VNTRVDWKETPEAHVFEADIPGLKKEEVKVQIEDDRVLQISGERNLEKEDKNDTWHRVE 120

Query: 121 --SGSFLSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDRSRRDIRVVEITG 152
             SG+F+ RF++PE+ K+E++ A M+ GVLTV VPKE+ + + D++ +EI+G
Sbjct: 121 RSSGNFMRRFRLPENAKVEQVKASMENGVLTVTVPKEEVK-KPDVKAIEISG 153

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy Swiss-Prot
Match: P02519 (17.3 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.3-B PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-23
Identity = 66/172 (38.37%), Postives = 97/172 (56.40%), Query Frame = 0


Query: 1   MSIIP--IGGRDGRISNTSSANILNHFPNFPFPLDLWHDF-PFPFPSSISRAFPDFAFGG 60
           MS+IP   GGR   + +             PF LD+W  F  FPFPSS+S     F    
Sbjct: 1   MSLIPSFFGGRRSSVFD-------------PFSLDVWDPFKDFPFPSSLSAENSAF---- 60

Query: 61  SVNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTE---------------- 120
            V+TR+DW ETP AHV +A +PG   ++V +E+QD R+LQIS E                
Sbjct: 61  -VSTRVDWKETPEAHVFKADIPGLKKEEVKLEIQDGRVLQISGERNVEKEDKNDTWHRVE 120

Query: 121 --SGSFLSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDRSRRDIRVVEITG 152
             SG  + RF++PE+ K++++ A M+ GVLTV VPKE+ + + D++ ++I+G
Sbjct: 121 RSSGKLVRRFRLPENAKVDQVKASMENGVLTVTVPKEEIK-KPDVKAIDISG 153

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy Swiss-Prot
Match: P27880 (18.2 kDa class I heat shock protein OS=Medicago sativa OX=3879 GN=HSP18.2 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-23
Identity = 69/175 (39.43%), Postives = 103/175 (58.86%), Query Frame = 0


Query: 1   MSIIP--IGGRDGRISNTSSANILNHFPNFPFPLDLWHDF-PFPF-PSSISRAFP--DFA 60
           MS+IP   GGR   + +             PF LD+W  F  FPF  S++S +FP  + A
Sbjct: 1   MSLIPSFFGGRRSNVFD-------------PFSLDVWDPFKDFPFNNSALSASFPRENSA 60

Query: 61  FGGSVNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTE------------- 120
           F   V+TR+DW ETP AHV +A LPG   ++V VE++DDR+LQIS E             
Sbjct: 61  F---VSTRVDWKETPEAHVFKADLPGMKKEEVKVEIEDDRVLQISGERSVEKEDKNDQWH 120

Query: 121 -----SGSFLSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDRSRRDIRVVEITG 152
                SG F+ RF++PE+ K++++ A M+ GVLTV VPKE+ + + +++ ++I+G
Sbjct: 121 RLERSSGKFMRRFRLPENAKMDQVKAAMENGVLTVTVPKEEVK-KPEVKTIDISG 158

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy Swiss-Prot
Match: P19243 (18.1 kDa class I heat shock protein OS=Pisum sativum OX=3888 GN=HSP18.1 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-22
Identity = 65/146 (44.52%), Postives = 92/146 (63.01%), Query Frame = 0


Query: 29  PFPLDLW---HDFPFPFPSSISRAFP--DFAFGGSVNTRLDWTETPNAHVLRASLPGFDG 88
           PF LD+W    DFPF   SS S +FP  + AF   V+TR+DW ETP AHV +A LPG   
Sbjct: 18  PFSLDVWDPLKDFPFS-NSSPSASFPRENPAF---VSTRVDWKETPEAHVFKADLPGLKK 77

Query: 89  DDVLVELQDDRMLQISTE------------------SGSFLSRFKIPESGKIEELSAFMD 148
           ++V VE++DDR+LQIS E                  SG FL RF++PE+ K++++ A M+
Sbjct: 78  EEVKVEVEDDRVLQISGERSVEKEDKNDEWHRVERSSGKFLRRFRLPENAKMDKVKASME 137

Query: 149 FGVLTVFVPKEDDRSRRDIRVVEITG 152
            GVLTV VPKE+ + + +++ +EI+G
Sbjct: 138 NGVLTVTVPKEEIK-KAEVKSIEISG 158

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy TrEMBL
Match: A0A6J1KBV9 (18.5 kDa class I heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC111492909 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.4e-72
Identity = 136/152 (89.47%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPIGG+DGRISN SS++ILN FP FPFPLDLWHD  FPFPSSIS AFPDFAFGGSVN
Sbjct: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHD--FPFPSSISHAFPDFAFGGSVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF G+DVLVELQDDRMLQIST+SG F+SRFKIPESGKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPKEDDRSRRDIRVVEITGE 153
           SAFMDFGVLTVFVPKEDD+SRRD+RVVEITGE
Sbjct: 121 SAFMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy TrEMBL
Match: A0A6J1GC89 (18.5 kDa class I heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111452661 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.4e-72
Identity = 136/152 (89.47%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPIGG+DGRISN SS++ILN FP FPFPLDLWHD  FPFPSSIS AFPDFAFGGSVN
Sbjct: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHD--FPFPSSISHAFPDFAFGGSVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF G+DVLVELQDDRMLQIST+SG F+SRFKIPESGKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPKEDDRSRRDIRVVEITGE 153
           SAFMDFGVLTVFVPKEDD+SRRD+RVVEITGE
Sbjct: 121 SAFMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy TrEMBL
Match: H6TB38 (HSP16.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.6e-66
Identity = 131/153 (85.62%), Postives = 141/153 (92.16%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPIGG+DGRISNTS +NILN FPNFPFPLDLWHD  FPFPSSIS     F++GG+VN
Sbjct: 1   MSIIPIGGQDGRISNTSPSNILNRFPNFPFPLDLWHD--FPFPSSIS---DPFSWGGTVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF G+DVLVELQDDRMLQISTESG F+SRFKIPE+GKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFGGEDVLVELQDDRMLQISTESGGFVSRFKIPETGKIEEL 120

Query: 121 SAFMDFGVLTVFVPKE-DDRSRRDIRVVEITGE 153
           SAFMDFG+LTVFVPKE DDRSRRD+RVVEITGE
Sbjct: 121 SAFMDFGILTVFVPKEDDDRSRRDVRVVEITGE 148

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy TrEMBL
Match: A0A5D3BEA9 (17.5 kDa class I heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001660 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.9e-63
Identity = 127/153 (83.01%), Postives = 136/153 (88.89%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPI G+DGR+SN S +NILN FPNFPFPLDLWHD  FPFPSSIS     F++G +VN
Sbjct: 1   MSIIPITGQDGRVSNPSPSNILNRFPNFPFPLDLWHD--FPFPSSIS---DPFSWGATVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF  +DVLVELQDDRMLQISTESG F+SRFKIPESGKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFGSEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPK-EDDRSRRDIRVVEITGE 153
           SAFMDFGVLTVFVPK EDDRS RD+RVVEITGE
Sbjct: 121 SAFMDFGVLTVFVPKDEDDRSGRDVRVVEITGE 148

[Close]

BLAST of Lcy10g001220.1 vs. ExPASy TrEMBL
Match: A0A1S3C660 (17.5 kDa class I heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC103497114 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.9e-63
Identity = 127/153 (83.01%), Postives = 136/153 (88.89%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPI G+DGR+SN S +NILN FPNFPFPLDLWHD  FPFPSSIS     F++G +VN
Sbjct: 1   MSIIPITGQDGRVSNPSPSNILNRFPNFPFPLDLWHD--FPFPSSIS---DPFSWGATVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF  +DVLVELQDDRMLQISTESG F+SRFKIPESGKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFGSEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPK-EDDRSRRDIRVVEITGE 153
           SAFMDFGVLTVFVPK EDDRS RD+RVVEITGE
Sbjct: 121 SAFMDFGVLTVFVPKDEDDRSGRDVRVVEITGE 148

[Close]

BLAST of Lcy10g001220.1 vs. NCBI nr
Match: KAG6607282.1 (18.5 kDa class I heat shock protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 281.6 bits (719), Expect = 4.1e-72
Identity = 136/152 (89.47%), Postives = 145/152 (95.39%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPIGG+DGRISN+SS++ILN FP FPFPLDLWHD  FPFPSSIS AFPDFAFGGSVN
Sbjct: 1   MSIIPIGGQDGRISNSSSSSILNRFPKFPFPLDLWHD--FPFPSSISHAFPDFAFGGSVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF G+DVLVELQDDRMLQIST+SG F+SRFKIPESGKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPKEDDRSRRDIRVVEITGE 153
           SAFMDFGVLTVFVPKEDD+SRRD+RVVEITGE
Sbjct: 121 SAFMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

[Close]

BLAST of Lcy10g001220.1 vs. NCBI nr
Match: XP_022949250.1 (18.5 kDa class I heat shock protein-like [Cucurbita moschata] >XP_022998185.1 18.5 kDa class I heat shock protein-like [Cucurbita maxima] >KAG7036961.1 18.5 kDa class I heat shock protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 280.4 bits (716), Expect = 9.1e-72
Identity = 136/152 (89.47%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPIGG+DGRISN SS++ILN FP FPFPLDLWHD  FPFPSSIS AFPDFAFGGSVN
Sbjct: 1   MSIIPIGGQDGRISNGSSSSILNRFPKFPFPLDLWHD--FPFPSSISHAFPDFAFGGSVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF G+DVLVELQDDRMLQIST+SG F+SRFKIPESGKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFQGEDVLVELQDDRMLQISTDSGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPKEDDRSRRDIRVVEITGE 153
           SAFMDFGVLTVFVPKEDD+SRRD+RVVEITGE
Sbjct: 121 SAFMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

[Close]

BLAST of Lcy10g001220.1 vs. NCBI nr
Match: XP_023524947.1 (18.5 kDa class I heat shock protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 279.3 bits (713), Expect = 2.0e-71
Identity = 135/152 (88.82%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPIGG+DGRISN+SS++I+N FP FPFPLDLWHD  FPFPSSIS AFPDFAFGGSVN
Sbjct: 1   MSIIPIGGQDGRISNSSSSSIVNRFPKFPFPLDLWHD--FPFPSSISHAFPDFAFGGSVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRA LPGF G+DVLVELQDDRMLQISTESG F+SRFKIPESGKIEEL
Sbjct: 61  TRLDWTETPNAHVLRAFLPGFQGEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPKEDDRSRRDIRVVEITGE 153
           SAFMDFGVLTVFVPKEDD+SRRD+RVVEITGE
Sbjct: 121 SAFMDFGVLTVFVPKEDDQSRRDVRVVEITGE 150

[Close]

BLAST of Lcy10g001220.1 vs. NCBI nr
Match: ADU55785.1 (HSP16.5 [Citrullus lanatus])

HSP 1 Score: 261.9 bits (668), Expect = 3.4e-66
Identity = 131/153 (85.62%), Postives = 141/153 (92.16%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPIGG+DGRISNTS +NILN FPNFPFPLDLWHD  FPFPSSIS     F++GG+VN
Sbjct: 1   MSIIPIGGQDGRISNTSPSNILNRFPNFPFPLDLWHD--FPFPSSIS---DPFSWGGTVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           TRLDWTETPNAHVLRASLPGF G+DVLVELQDDRMLQISTESG F+SRFKIPE+GKIEEL
Sbjct: 61  TRLDWTETPNAHVLRASLPGFGGEDVLVELQDDRMLQISTESGGFVSRFKIPETGKIEEL 120

Query: 121 SAFMDFGVLTVFVPKE-DDRSRRDIRVVEITGE 153
           SAFMDFG+LTVFVPKE DDRSRRD+RVVEITGE
Sbjct: 121 SAFMDFGILTVFVPKEDDDRSRRDVRVVEITGE 148

[Close]

BLAST of Lcy10g001220.1 vs. NCBI nr
Match: XP_038894363.1 (17.5 kDa class I heat shock protein-like [Benincasa hispida])

HSP 1 Score: 250.8 bits (639), Expect = 7.8e-63
Identity = 126/153 (82.35%), Postives = 137/153 (89.54%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MSIIPI G+DGRIS+TS +NILN FPNFPFPLDLWH   FPFPSSIS     F++GG+VN
Sbjct: 1   MSIIPIAGQDGRISSTSPSNILNRFPNFPFPLDLWHG--FPFPSSIS---DPFSWGGNVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESGKIEEL 120
           T LDWTETPNAHV+RASLPGF  +DVLVELQDDRMLQISTESG F+SRFKIPESGKIEEL
Sbjct: 61  THLDWTETPNAHVVRASLPGFGSEDVLVELQDDRMLQISTESGGFVSRFKIPESGKIEEL 120

Query: 121 SAFMDFGVLTVFVPK-EDDRSRRDIRVVEITGE 153
           SAFMDFG+LTVFVPK EDDRSRRD+RVVEITGE
Sbjct: 121 SAFMDFGILTVFVPKEEDDRSRRDVRVVEITGE 148

[Close]

BLAST of Lcy10g001220.1 vs. TAIR 10
Match: AT2G19310.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 4.9e-23
Identity = 66/166 (39.76%), Postives = 94/166 (56.63%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSA----NILNHFPNFPFP-LDLWHDFPFPFPSSISRAFPDFAF 60
           MS+IPI  R  R+S          ++N F +FP P L L H FP     S+SR       
Sbjct: 1   MSMIPISNR-RRLSPGDRIWEPFELMNTFLDFPSPALFLSHHFP-----SLSREIFPQTS 60

Query: 61  GGSVNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTESGSFLSRFKIPESG 120
             +VNT+L+WTETP AHV +A LPG D D+V+  + ++  LQI T    F+SRFK+P + 
Sbjct: 61  SSTVNTQLNWTETPTAHVFKAYLPGVDQDEVIAFVDEEGYLQICTGDNKFMSRFKLPNNA 120

Query: 121 KIEELSAFMDFGVLTVFVPKEDDRS---------RRDIRVVEITGE 153
             ++++A+M+   L VFV K+   S          R++RVVEITG+
Sbjct: 121 LTDQVTAWMEDEFLVVFVEKDASSSPPQLPEIEENRNVRVVEITGD 160

[Close]

BLAST of Lcy10g001220.1 vs. TAIR 10
Match: AT2G29500.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 4.6e-21
Identity = 61/169 (36.09%), Postives = 92/169 (54.44%), Query Frame = 0


Query: 1   MSIIPIGGRDGRISNTSSANILNHFPNFPFPLDLWHDFPFPFPSSISRAFPDFAFGGSVN 60
           MS+IP        +N   +NI +     PF LD+W  F     SS+SR          VN
Sbjct: 1   MSMIP-----SFFNNNRRSNIFD-----PFSLDVWDPFKELTSSSLSR-----ENSAIVN 60

Query: 61  TRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTE------------------S 120
            R+DW ETP AHV +A LPG   ++V VE+++D +L+IS E                  S
Sbjct: 61  ARVDWRETPEAHVFKADLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSS 120

Query: 121 GSFLSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDRSRRDIRVVEITG 152
           G F  RF++PE+ K++++ A M+ GVLTV VPK + + + D++ ++I+G
Sbjct: 121 GQFTRRFRLPENVKMDQVKAAMENGVLTVTVPKAETK-KADVKSIQISG 153

[Close]

BLAST of Lcy10g001220.1 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 7.8e-21
Identity = 64/172 (37.21%), Postives = 94/172 (54.65%), Query Frame = 0


Query: 1   MSIIP-IGGRDGRISNTSSANILNHFPNFPFPLDLWHDF-PFPFPSSISRAFPDFAFGGS 60
           MS+IP   G + RI+N    NI +     PF LD+W  F    FPSS S A         
Sbjct: 1   MSLIPSFFGNNRRINN----NIFD-----PFSLDVWDPFKELQFPSSSSSAI-------- 60

Query: 61  VNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTE----------------- 120
            N R+DW ET  AHV +A LPG   ++V VE++DD +L+IS E                 
Sbjct: 61  ANARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVER 120

Query: 121 -SGSFLSRFKIPESGKIEELSAFMDFGVLTVFVPK-EDDRSRRDIRVVEITG 152
            SG F  +F++PE+ K++++ A M+ GVLTV VPK E ++ +  ++ ++I+G
Sbjct: 121 SSGGFSRKFRLPENVKMDQVKASMENGVLTVTVPKVETNKKKAQVKSIDISG 155

[Close]

BLAST of Lcy10g001220.1 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 96.7 bits (239), Expect = 1.7e-20
Identity = 61/173 (35.26%), Postives = 89/173 (51.45%), Query Frame = 0


Query: 1   MSIIP--IGGRDGRISNTSSANILNHFPNFPFPLDLWHDFP--FPFPSSISRAFPDFAFG 60
           MS+IP   GGR   + +             PF  DLW  F   F   S+++ A       
Sbjct: 1   MSLIPSIFGGRRSNVFD-------------PFSQDLWDPFEGFFTPSSALANASTARDVA 60

Query: 61  GSVNTRLDWTETPNAHVLRASLPGFDGDDVLVELQDDRMLQISTE--------------- 120
              N R+DW ETP AHV +A LPG   ++V VE++D  +LQIS E               
Sbjct: 61  AFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRV 120

Query: 121 ---SGSFLSRFKIPESGKIEELSAFMDFGVLTVFVPKEDDRSRRDIRVVEITG 152
              SG F+ RF++PE+ K+EE+ A M+ GVLTV VPK  ++ +  ++ ++I+G
Sbjct: 121 ERASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPKAPEK-KPQVKSIDISG 159

[Close]

BLAST of Lcy10g001220.1 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.1e-19
Identity = 54/143 (37.76%), Postives = 80/143 (55.94%), Query Frame = 0


Query: 29  PFPLDLWHDF-PFPFPSSISRAFPDFAFGGSVNTRLDWTETPNAHVLRASLPGFDGDDVL 88
           PF LD+W  F    FPSS+S            N R+DW ET  AHV +A LPG   ++V 
Sbjct: 20  PFSLDVWDPFKELQFPSSLSG-----ETSAITNARVDWKETAEAHVFKADLPGMKKEEVK 79

Query: 89  VELQDDRMLQISTE------------------SGSFLSRFKIPESGKIEELSAFMDFGVL 148
           VE++DD +L+IS E                  SG F  +FK+PE+ K++++ A M+ GVL
Sbjct: 80  VEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASMENGVL 139

Query: 149 TVFVPK-EDDRSRRDIRVVEITG 152
           TV VPK E+ + +  ++ ++I+G
Sbjct: 140 TVTVPKVEEAKKKAQVKSIDISG 157

The following BLAST results are available for this feature:

BLAST of Lcy10g001220.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05478	2.7e-26	40.00	18.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP18.5-C PE=3 SV=...	[more]
P04793	1.9e-24	39.53	17.5 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.5-M PE=3 SV=...	[more]
P02519	2.8e-23	38.37	17.3 kDa class I heat shock protein OS=Glycine max OX=3847 GN=HSP17.3-B PE=3 SV=...	[more]
P27880	2.8e-23	39.43	18.2 kDa class I heat shock protein OS=Medicago sativa OX=3879 GN=HSP18.2 PE=2 S...	[more]
P19243	1.4e-22	44.52	18.1 kDa class I heat shock protein OS=Pisum sativum OX=3888 GN=HSP18.1 PE=1 SV=...	[more]

BLAST of Lcy10g001220.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KBV9	4.4e-72	89.47	18.5 kDa class I heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1GC89	4.4e-72	89.47	18.5 kDa class I heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
H6TB38	1.6e-66	85.62	HSP16.5 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A5D3BEA9	4.9e-63	83.01	17.5 kDa class I heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3C660	4.9e-63	83.01	17.5 kDa class I heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC103497114...	[more]

BLAST of Lcy10g001220.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6607282.1	4.1e-72	89.47	18.5 kDa class I heat shock protein, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022949250.1	9.1e-72	89.47	18.5 kDa class I heat shock protein-like [Cucurbita moschata] >XP_022998185.1 18...	[more]
XP_023524947.1	2.0e-71	88.82	18.5 kDa class I heat shock protein-like [Cucurbita pepo subsp. pepo]	[more]
ADU55785.1	3.4e-66	85.62	HSP16.5 [Citrullus lanatus]	[more]
XP_038894363.1	7.8e-63	82.35	17.5 kDa class I heat shock protein-like [Benincasa hispida]	[more]

BLAST of Lcy10g001220.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G19310.1	4.9e-23	39.76	HSP20-like chaperones superfamily protein	[more]
AT2G29500.1	4.6e-21	36.09	HSP20-like chaperones superfamily protein	[more]
AT1G59860.1	7.8e-21	37.21	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	1.7e-20	35.26	heat shock protein 18.2	[more]
AT1G07400.1	1.1e-19	37.76	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 64..149 e-value: 1.1E-7 score: 31.9
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 54..152 score: 14.111392
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 26..149 e-value: 3.1E-17 score: 64.6
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 34..146
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 21..151
None	No IPR available	PANTHER	PTHR11527:SF320	17.5 KDA CLASS I HEAT SHOCK PROTEIN	coord: 21..151

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy10g001220	Lcy10g001220	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy10g001220.1.utr5p1	Lcy10g001220.1.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy10g001220.1.exon1	Lcy10g001220.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy10g001220.1.cds1	Lcy10g001220.1.cds1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy10g001220.1.utr3p1	Lcy10g001220.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy10g001220.1	Lcy10g001220.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding