Lcy09g002910.1 (mRNA) Sponge gourd (P93075) v1

Overview
NameLcy09g002910.1
TypemRNA
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionProline dehydrogenase
LocationChr09: 2472934 .. 2476428 (-)
Sequence length1803
RNA-Seq ExpressionLcy09g002910.1
SyntenyLcy09g002910.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAACAAAACAAAACAAAGAAGAGCAAATTAAAAAAAAAAGAAAAAAAAAAGTGAATTTTTTCTGCAAATTTTGATGGCTAGAAGAATTGTAGCAACCCAATCAAAGATCCCCAAAAAGGTCTTTCTTTACACATTCAATCGGCCGTTGAACACCGCCGCCTCCTCCTCCGCCGCCGCCTTCACCGCCGTCGCCGCCCCGCCGCCGTCGCTCAACCTCGCCGGCTCTCCGGCGACGCCAAGTGTGGATTTCGCCGACACGAGGGCCTTGTTTGGGTCCCTTCCGACGTCGGATCTCCTACGCGCCGCCGCCAACCTCCACGTCGCCGCCGTCGGCCCCGTCGTGGACGTCGGGATGTGGGTCATGAAGTCCAAGTTGATGGAGGTGGAGTTGGCTCGTGATGCGATTTTGGGTGCCGTCAAGCATACATTTTACCGACATTTTTGCGCCGGCGAGGACGACGCGGCGGTCGGAGAAACCGTCCGGCGGTTGCACAACGCCGGGCTCCGGAGCATGCTCGATTATGCCCTGGAATACGCCGACGACGAGGAGTCTTGTGATCGGAATCTTGAAGGGTTTCTTCGAACCATCGACGCCACGAAATCGCTGCCGCTTGGCTCCGTAAGTTTCCACGATCTTCTTCTTCAACTTTCTTGTTTCTTCTATTTTCGTCAATGTTTTTTTAAAAATGTTTATTCAAATTCTGGAATTTTCAATTAATGTTACTCTAAAGCAAATATATTTTGTTTTCTCTAAAATTATGTTTATTTTGTTTTAATTAGTATATTTTAATAGGATTCGAATTTAACTTATAAATTACGCCATTTGTATTTATTGATAAGCTCTGATTTTTTATTTTTTTATTTTTTTATTTTTTAGAAAGATAAGCTCTGATTTGTGTGTGCCAGAAGCAGAAGTTTTATATTTTTTAAAATATAAGAGGACAAATACAAGATCAGGTTATTATTAAAAAAAAAAAAAATCAGGCATTTTCCACGAGTTTTTGTTCTGTTCTGACAATTTTTTTTTTTTTTTTTTTTTTTTTTAAGTTCTGGGCTTTTTTTTTTGTTGAAAAAAAATTGGCATATTTTTTTGAAGCTATGTACTGGCATATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAATGTTCTGTCATGTTTTTAATTTTACTTTTATAGAATAGTAAATGTTTGAGTTGCAAATATTAGATCTTATTATTATTTTTTTTTTTTGAAATTAATATGAGATCTTATTTATTATTTCTTTACGTTATTCTAGTTGAAATATAGGGTAGTAATTAAAAAGGTTTGAATATAAGAAAGAATAATAACGGTTGTAATCTTCTAAAGAAAGAATAATAATTGTTGTAATCTACTAAAAAAATAATAATAATTGTTGTAATTTTTTCCAATAAAAAAAAAATTGGTTTTTTTATTTCTAGAATGGTCAATGGTCAAACTTTTAAGCAAAACAAAAGAATAGATAAGAATTTTACTGCCACGTGCTTTCTCTTATGGATAATACATTGCCACGTAGATATTTGATGTCTTTATTTCCACCCATTAATTTTTCTTGTTAAGTTAGTAGCCCGATTTCCAAAACTATGGGATTTGTTTAAAAAATGCTCATGAACTTTGAAAAGTTTTTCAAAAATATCCTTTTCATTAATTTTTGGATGGAACCATTAGAATTTACGGTGAGATGAGGAAAAAAGAAGAAGCATTTTTCAATAAGGGTATTTTTTAATTTTTTTTGAAAGTTCAAGGATATTTTTTAGACTGAAACTTTAACGGTTTCCATCCAAAAATCAACAAGGTATTTTTGAAACATTTTTCAAAGTTCAGTGGTATCTTTGAAACTTTTCAAAGTTCAGAGGCATTTTTGAAACAAACCCCATAGTTTAGGGGCATTTTTTTTTATATAATTTAGTCAACATTTTAATTTCATCTGTATAAAAAGAAAAGTTGATCAAGCAACTTGAAATGGTGATGAAATTGAATTATTTGTTCATTTGGGTGTTGTTTATTGATGGGTCATTTTCTTTTCCTAAACTCTTCCAATTTGGCAACTTTTCTGTTTGTTTCCTGAGAAAATGAAAATTCTTTGTATTTTGATAGGCAAGTTTTGTGGTGACAAAAGTGAGTGCAATTTGTCCATTGAAATTGCTTGAGAGAGTTAGTGATTTGTTAAGATGGCAACAAAAGAACCCTTGTTTCAATCTCCCATGGAAGCTCAAAACTTTCCCAATTCTCTCTCAATCAAGCCCTCTCTACCACACTCTTGAACAACCCAATCCCTTGACACCTCAAGAAGAGAAAAGCCTCCAAATTAGCCACCAAAGGCTCATGAAGATCTGTCAAAGTTGTGTTGATGCCAACGTCCCGTTGGCCATCGATGCCGAACACACCAAAGTTCAACCCGCCATCGATTACTTCACTTATTCCGCCGCCATCATCCACAACAGAGACGATAATCCGATCGTCTACGGAACCGTTCAAGCGTATCTGAAGGATGCAAAAGATCGACTGTTGGCAGTTACCAAGGCTGCCGACGACATGAGAGTTCCGCTCGGGATCAAGCTCGTCAGGGGTGCTTACATGTCGAGCGAATGCAAACTCGCTTCTTCGTTAGGGTTCGAATCTCCCATTCACAACAACATTCAGGACACGCATTCGTGCTACAACAGCTGCGCGTCTTACTTACTCGAGAACATTGCCAAGGGCTCGAGTGGAGTCATTCTCGCAACTCACAATGTGGAATCAGGTAAGACGAATTGCGCTTCGAGCGTTTTCTGTTTGTTGTCATTGTTTTTAGGGTTTAAGGAGTATGTTTATTGAAAAGTTGATGGTGTGTTTTGAGTAGGGAAATTGGCAGCTAGCAAAGCATATGAGATAGGGATTGGAAAGTTGAAGCAAAAGATTGAGTTTGCCCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGCAAATACATGCCATTTGGACTAGTGGATATGGTAATGCCATACCTTTTGAGAAGGGCTGAAGAGAATAGAGGTCTCTTATCTACTTCAAATTTGGATAGAAAACTTATGAGGTAAAATTTATATATATATATATATATTTATATTTTTCTCATCTCACAAATTCAAAATAGCTGAAATTTTTGTGTTGATATTTGATATTTGCAGAAAGGAGTTGGCAAGAAGGATGAAAGATTACTTGAGGTGAAAGAAGGGCAATTTAGCAACAAACTTCAACTTCACTAATGTTTTGAAAATGTACTCTTTTTTTTTTTTTTATTTTACAATTTTCATTTATGTTTTGGAAGTTTAATATATATCGTTTCCTTTTTTATTTTTGCTTTTTGAATTTAGGTCTATTCGTAAAGTTCGTTTTGATCTCACTAAAACCATACAAACTATTATTGAAATGTACTATTATATTATAACTATGATAATTAAGACCCTAAATTAACATCG

mRNA sequence

AAAAAAAAACAAAACAAAACAAAGAAGAGCAAATTAAAAAAAAAAGAAAAAAAAAAGTGAATTTTTTCTGCAAATTTTGATGGCTAGAAGAATTGTAGCAACCCAATCAAAGATCCCCAAAAAGGTCTTTCTTTACACATTCAATCGGCCGTTGAACACCGCCGCCTCCTCCTCCGCCGCCGCCTTCACCGCCGTCGCCGCCCCGCCGCCGTCGCTCAACCTCGCCGGCTCTCCGGCGACGCCAAGTGTGGATTTCGCCGACACGAGGGCCTTGTTTGGGTCCCTTCCGACGTCGGATCTCCTACGCGCCGCCGCCAACCTCCACGTCGCCGCCGTCGGCCCCGTCGTGGACGTCGGGATGTGGGTCATGAAGTCCAAGTTGATGGAGGTGGAGTTGGCTCGTGATGCGATTTTGGGTGCCGTCAAGCATACATTTTACCGACATTTTTGCGCCGGCGAGGACGACGCGGCGGTCGGAGAAACCGTCCGGCGGTTGCACAACGCCGGGCTCCGGAGCATGCTCGATTATGCCCTGGAATACGCCGACGACGAGGAGTCTTGTGATCGGAATCTTGAAGGGTTTCTTCGAACCATCGACGCCACGAAATCGCTGCCGCTTGGCTCCGCAAGTTTTGTGGTGACAAAAGTGAGTGCAATTTGTCCATTGAAATTGCTTGAGAGAGTTAGTGATTTGTTAAGATGGCAACAAAAGAACCCTTGTTTCAATCTCCCATGGAAGCTCAAAACTTTCCCAATTCTCTCTCAATCAAGCCCTCTCTACCACACTCTTGAACAACCCAATCCCTTGACACCTCAAGAAGAGAAAAGCCTCCAAATTAGCCACCAAAGGCTCATGAAGATCTGTCAAAGTTGTGTTGATGCCAACGTCCCGTTGGCCATCGATGCCGAACACACCAAAGTTCAACCCGCCATCGATTACTTCACTTATTCCGCCGCCATCATCCACAACAGAGACGATAATCCGATCGTCTACGGAACCGTTCAAGCGTATCTGAAGGATGCAAAAGATCGACTGTTGGCAGTTACCAAGGCTGCCGACGACATGAGAGTTCCGCTCGGGATCAAGCTCGTCAGGGGTGCTTACATGTCGAGCGAATGCAAACTCGCTTCTTCGTTAGGGTTCGAATCTCCCATTCACAACAACATTCAGGACACGCATTCGTGCTACAACAGCTGCGCGTCTTACTTACTCGAGAACATTGCCAAGGGCTCGAGTGGAGTCATTCTCGCAACTCACAATGTGGAATCAGGGAAATTGGCAGCTAGCAAAGCATATGAGATAGGGATTGGAAAGTTGAAGCAAAAGATTGAGTTTGCCCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGCAAATACATGCCATTTGGACTAGTGGATATGGTAATGCCATACCTTTTGAGAAGGGCTGAAGAGAATAGAGGTCTCTTATCTACTTCAAATTTGGATAGAAAACTTATGAGAAAGGAGTTGGCAAGAAGGATGAAAGATTACTTGAGGTGAAAGAAGGGCAATTTAGCAACAAACTTCAACTTCACTAATGTTTTGAAAATGTACTCTTTTTTTTTTTTTTATTTTACAATTTTCATTTATGTTTTGGAAGTTTAATATATATCGTTTCCTTTTTTATTTTTGCTTTTTGAATTTAGGTCTATTCGTAAAGTTCGTTTTGATCTCACTAAAACCATACAAACTATTATTGAAATGTACTATTATATTATAACTATGATAATTAAGACCCTAAATTAACATCG

Coding sequence (CDS)

ATGGCTAGAAGAATTGTAGCAACCCAATCAAAGATCCCCAAAAAGGTCTTTCTTTACACATTCAATCGGCCGTTGAACACCGCCGCCTCCTCCTCCGCCGCCGCCTTCACCGCCGTCGCCGCCCCGCCGCCGTCGCTCAACCTCGCCGGCTCTCCGGCGACGCCAAGTGTGGATTTCGCCGACACGAGGGCCTTGTTTGGGTCCCTTCCGACGTCGGATCTCCTACGCGCCGCCGCCAACCTCCACGTCGCCGCCGTCGGCCCCGTCGTGGACGTCGGGATGTGGGTCATGAAGTCCAAGTTGATGGAGGTGGAGTTGGCTCGTGATGCGATTTTGGGTGCCGTCAAGCATACATTTTACCGACATTTTTGCGCCGGCGAGGACGACGCGGCGGTCGGAGAAACCGTCCGGCGGTTGCACAACGCCGGGCTCCGGAGCATGCTCGATTATGCCCTGGAATACGCCGACGACGAGGAGTCTTGTGATCGGAATCTTGAAGGGTTTCTTCGAACCATCGACGCCACGAAATCGCTGCCGCTTGGCTCCGCAAGTTTTGTGGTGACAAAAGTGAGTGCAATTTGTCCATTGAAATTGCTTGAGAGAGTTAGTGATTTGTTAAGATGGCAACAAAAGAACCCTTGTTTCAATCTCCCATGGAAGCTCAAAACTTTCCCAATTCTCTCTCAATCAAGCCCTCTCTACCACACTCTTGAACAACCCAATCCCTTGACACCTCAAGAAGAGAAAAGCCTCCAAATTAGCCACCAAAGGCTCATGAAGATCTGTCAAAGTTGTGTTGATGCCAACGTCCCGTTGGCCATCGATGCCGAACACACCAAAGTTCAACCCGCCATCGATTACTTCACTTATTCCGCCGCCATCATCCACAACAGAGACGATAATCCGATCGTCTACGGAACCGTTCAAGCGTATCTGAAGGATGCAAAAGATCGACTGTTGGCAGTTACCAAGGCTGCCGACGACATGAGAGTTCCGCTCGGGATCAAGCTCGTCAGGGGTGCTTACATGTCGAGCGAATGCAAACTCGCTTCTTCGTTAGGGTTCGAATCTCCCATTCACAACAACATTCAGGACACGCATTCGTGCTACAACAGCTGCGCGTCTTACTTACTCGAGAACATTGCCAAGGGCTCGAGTGGAGTCATTCTCGCAACTCACAATGTGGAATCAGGGAAATTGGCAGCTAGCAAAGCATATGAGATAGGGATTGGAAAGTTGAAGCAAAAGATTGAGTTTGCCCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGCAAATACATGCCATTTGGACTAGTGGATATGGTAATGCCATACCTTTTGAGAAGGGCTGAAGAGAATAGAGGTCTCTTATCTACTTCAAATTTGGATAGAAAACTTATGAGAAAGGAGTTGGCAAGAAGGATGAAAGATTACTTGAGGTGA

Protein sequence

MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAAPPPSLNLAGSPATPSVDFADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTFYRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLPLGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQPNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRDDNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPIHNNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEFAQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKELARRMKDYLR
Homology
BLAST of Lcy09g002910.1 vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 516.2 bits (1328), Expect = 4.2e-145
Identity = 257/432 (59.49%), Postives = 325/432 (75.23%), Query Frame = 0

Query: 57  VDFADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVK 116
           +D +D   LF S+PTSDLLR+ A LH AA+GP+VD+G WVM SKLM+  + R  +LG VK
Sbjct: 63  LDLSDQARLFSSIPTSDLLRSTAVLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVK 122

Query: 117 HTFYRHFCAGEDDAAVGETVRRLHNA-GLRSMLDYALEYADDEESCDRNLEGFLRTIDAT 176
            TFY HFCAGED  A  E VR ++ A GL+ ML Y +E+ADD  SCD N++ F+RTI+A 
Sbjct: 123 STFYDHFCAGEDADAAAERVRSVYEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAA 182

Query: 177 KSLPLGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYH 236
           KSLP    S VV K++AICP+ LL+RVSDLLRW+ K+P F L WKLK+FP+ S+SSPLYH
Sbjct: 183 KSLPTSHFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYH 242

Query: 237 TLEQPNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 296
           T  +P PLT +EE+ L+ +H R+ +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI+
Sbjct: 243 TNSEPEPLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIM 302

Query: 297 HNRD-DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLG 356
            N D D PIVY T+QAYL+DA +RL    + A+   VP+G KLVRGAYMSSE  LA SLG
Sbjct: 303 FNADKDRPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLG 362

Query: 357 FESPIHNNIQDTHSCYNSCASYLLENIAKGSS-GVILATHNVESGKLAASKAYEIGIGKL 416
            +SP+H+ IQDTHSCYN C ++L+E  + GS  GV+LATHN +SG+LA+ KA ++GI K 
Sbjct: 363 CKSPVHDTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQ 422

Query: 417 KQKIEFAQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLD 476
             KIEFAQLYGMS+ALSFGL+ AGF VSKYMPFG V   +PYLLRRA ENRG+++T   D
Sbjct: 423 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHD 482

Query: 477 RKLMRKELARRM 486
           R+LMR EL RR+
Sbjct: 483 RQLMRMELKRRL 494

BLAST of Lcy09g002910.1 vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-141
Identity = 249/432 (57.64%), Postives = 319/432 (73.84%), Query Frame = 0

Query: 57  VDFADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVK 116
           +D +D   LF S+P S LLR+ A LH  ++GP+VD+G W+M SKLM+  + RD +L  VK
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 117 HTFYRHFCAGEDDAAVGETVRRLH-NAGLRSMLDYALEYADDEESCDRNLEGFLRTIDAT 176
            TFY HFCAGED AA    V  ++ + GL+ ML Y +E+A+D  +CD N++ F+ T++A 
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 177 KSLPLGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYH 236
           K+LP    S VV K++AICP+ +L+RVSDLLRWQ KNP F LPWKL +FP+ S  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 237 TLEQPNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 296
           T  +P PLT +EE+ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI+
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 297 HNRD-DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLG 356
            N D D PIVY T+QAYLKDA +RL    + ++ M VP+G KLVRGAYMSSE KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 357 FESPIHNNIQDTHSCYNSCASYLLENIAKGSS-GVILATHNVESGKLAASKAYEIGIGKL 416
           ++SP+H+ IQ+TH CYN C S+L+E  + GS   VILATHN +SGKL A KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 417 KQKIEFAQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLD 476
             KIEFAQLYGMS+ALSFGL+ AGF VSKYMP+G VD  +PYL+RRA ENRG++ST  LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

Query: 477 RKLMRKELARRM 486
           R+LMRKEL RR+
Sbjct: 462 RQLMRKELKRRV 473

BLAST of Lcy09g002910.1 vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 7.6e-46
Identity = 140/441 (31.75%), Postives = 220/441 (49.89%), Query Frame = 0

Query: 61  DTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTFY 120
           DT  L+ S  T +L      L V ++  + D     +   L E       +   +K++F+
Sbjct: 145 DTSKLYVSKSTGELFFTFMILKVCSINFISDNSQKFL--NLFEKLGLSKPLNFFIKYSFF 204

Query: 121 RHFCAGEDDAAVGETVRRLHNAGLRSMLDYAL-EYADDEESCDRNLEGFLRTIDATKSLP 180
           + FCAGE          +L+  G+ ++LDYA+ E A   E  D   E    TI      P
Sbjct: 205 KQFCAGETIRETEIFTEKLNKLGIGTILDYAIEELAGSSEGFDSVAENICETIRVAAKNP 264

Query: 181 LGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQ 240
             S S V  K + +    +LE+++ L+         N+   +   PI S +SPL   L Q
Sbjct: 265 TNSFSCV--KFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQ 324

Query: 241 PNP---------LTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTY 300
            +          LT ++ K ++    R+ KI Q C    VP+ +DAE +  Q AI + T 
Sbjct: 325 SSSLMKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTM 384

Query: 301 SAAIIHNRDDNPIVYGTVQAYLKDAKDRLLA--VTKAADDMRVPLGIKLVRGAYMSSECK 360
           S +I +N+ + PI+Y T Q YL +  + L       ++      LG K+VRGAYM +E +
Sbjct: 385 SYSIKYNK-EKPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESE 444

Query: 361 LASSLGFESPIHNNIQDTHSCYNSCASYLLENIAK--GSSGVILATHNVESGKLAASKAY 420
            +  L  E+P+   IQDTH  YN+   +LL  I     S G+++A+HN +S  L      
Sbjct: 445 RSQRLSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIK 504

Query: 421 EIGIGKLKQKIEFAQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGL 480
           +  I      I+F QL+GM++ LSF L +   ++ KY+PFG V+ V+PYL+RR  EN+G 
Sbjct: 505 QYKIDPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKGF 564

Query: 481 LSTSNLDRKL--MRKELARRM 486
           +  SN D++L  ++KE+ RR+
Sbjct: 565 IG-SNSDKELFYLKKEIKRRL 571

BLAST of Lcy09g002910.1 vs. ExPASy Swiss-Prot
Match: O45228 (Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh-1 PE=2 SV=2)

HSP 1 Score: 161.0 bits (406), Expect = 3.4e-38
Identity = 141/551 (25.59%), Postives = 236/551 (42.83%), Query Frame = 0

Query: 57  VDFADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVK 116
           + F +T+  F S   ++L+RA   L +  +  +V+    ++ +  M   L ++     +K
Sbjct: 59  LSFENTKEAFKSKSNTELVRALVVLRLCGIQTLVNQNQIILNT--MRRVLGKNLFKKTLK 118

Query: 117 HTFYRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALE----------------------- 176
           +TF+ HF AGE +  V   V +L N G++S+LDY++E                       
Sbjct: 119 NTFFGHFVAGETEEEVRHVVEKLRNYGVKSILDYSVEADITSQEATDKTVKGTSVATVKP 178

Query: 177 --------------------------------------YADDEESCDRNLEGFLRTIDAT 236
                                                 + + EE CD+N + F  +I+A 
Sbjct: 179 AAMTPVVDAKTLETTRERYTVHEEFGDRRQGVSSARTYFYEGEEQCDKNRDIFKDSINAV 238

Query: 237 KSLPLGSASFVVTKVSAICPLKLLERVSDLLRWQQK------------------------ 296
            S    +  FV  K++A+   +LL ++S+ +   Q                         
Sbjct: 239 AS-ATKNEGFVAVKITALGRPQLLLKLSEAIVQTQNFFKALTGGMSLQEGRLTSQEFYKR 298

Query: 297 ---------------------------------NPCFNLPWKL-KTFPILSQSSPLYHTL 356
                                            N   +   KL + F +L+  +     L
Sbjct: 299 LGELGVKTDTESVKKFFDEVDFDSDGIVDLHGWNHILDDHVKLGQLFQVLNIKTGSLEPL 358

Query: 357 EQPNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN 416
            Q   L+ +EE+  +   +R + + +  ++  V + +DAE T +QPAI   T      +N
Sbjct: 359 IQ--NLSNEEEQEFRNMVRRTLDVAEYAIEKGVRIMVDAEQTYLQPAISKITIEMMKKYN 418

Query: 417 RDDNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFES 476
           +    I + T QAYLK     + A  + A       G KLVRGAYM  E   A ++G+E 
Sbjct: 419 KGRGNI-FNTYQAYLKGTLQNMEADMQVARREGWHFGAKLVRGAYMEQERARAKAIGYED 478

Query: 477 PIHNNIQDTHSCYNSCASYLLENI---AKGSSGVILATHNVESGKLAASKAYEIGIGKLK 486
           PI++N + T   Y SC + + + +    K +  V++A+HN ++ + A +   E  I   +
Sbjct: 479 PINDNFEATSKMYESCLTRIADEVHRRGKTNVSVMVASHNEDTVRFALNLMKEKCISPSE 538

BLAST of Lcy09g002910.1 vs. ExPASy Swiss-Prot
Match: Q04499 (Proline dehydrogenase 1, mitochondrial OS=Drosophila melanogaster OX=7227 GN=slgA PE=2 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 9.9e-38
Identity = 147/563 (26.11%), Postives = 242/563 (42.98%), Query Frame = 0

Query: 49  AGSPATPSVD--FADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVEL 108
           AGSP    +D  F D  A F S  T +L+RA     + +   +V+  M +M  KL    L
Sbjct: 106 AGSPQRDPLDVSFNDPIAAFKSKTTGELIRAYLVYMICSSEKLVEHNMTLM--KLARNLL 165

Query: 109 ARDAILGAVKHTFYRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALE------------- 168
            +   +  +K +FY HF AGE+   +   + RL + G++ +LDY++E             
Sbjct: 166 GQKLFVLLMKSSFYGHFVAGENRHTIVPALERLRSFGVKPILDYSVEEDITQEEAEKREV 225

Query: 169 ------------------------YAD--------------DEESCDRNLEGFLRTIDAT 228
                                   +AD              +E +C+RN+E F++ ++A 
Sbjct: 226 ESSVSSAGDKKEEGSMPQYHVDKSFADRRYKVSSARTYFYLNEATCERNMEIFIKCLEAV 285

Query: 229 --------KSLPLGS---ASFVVTKVSAICPLKLLERVSDLLRWQQK------------- 288
                   +++  G+         K++A+   +LL ++S+++   +K             
Sbjct: 286 SDDDRKAPRAVATGATFGTGITAIKLTALGRPQLLLQLSEVIMRTRKYMEDMVGGQGNVL 345

Query: 289 ---------------------------NPCFNLPWKLKTFP---ILSQSSPLYHTLEQPN 348
                                      N   +    L  FP   I+ + S L  T   P+
Sbjct: 346 THHKTIKDLEKYYATLGDNKDVKEFLNNVTSDKEGILHLFPWSGIVDEDSQLSDTFRVPD 405

Query: 349 PLT-----------PQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTY 408
           P T           P+EE+  +   +RL  I ++  D +V + +DAE T  QPAI   T 
Sbjct: 406 PQTGQMRRLISQIPPKEEEMFRNMIRRLNTIVKAAADLDVRIMVDAEQTYFQPAISRITL 465

Query: 409 SAAIIHNRDDNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLA 468
                +N+ D  IV+ T Q YL++    +    + A       G KLVRGAYM  E   A
Sbjct: 466 EMMRKYNK-DKAIVFNTYQCYLRETFREVNTDLEQAKRQNFYFGAKLVRGAYMDQERDRA 525

Query: 469 SSLGFESPIHNNIQDTHSCYNSCASYLLENIA--------KGSSGVILATHNVESGKLAA 486
            SLG+  P++   + T   Y+   S  L  I             G+++A+HN ++ + A 
Sbjct: 526 KSLGYPDPVNPTFEATTDMYHRTLSECLRRIKLMKDCDDDARKIGIMVASHNEDTVRFAI 585

BLAST of Lcy09g002910.1 vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.7e-234
Identity = 414/491 (84.32%), Postives = 453/491 (92.26%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAA-PPPSLNLAGSPATPSVDF 60
           MAR I++TQSKIPK VF Y FNR LNTAASSSAAAFT+ A+ PPP LNLA SPA P+VDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  ADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTF 120
           +DT+ALFGSL T ++L AAA+LHVAAVGPVVDVGMWVM SKLM+VELARD I G V+H+F
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLP 180
           YRHFCAGEDD AVG+TVRRLHN GLRSMLDYA+EYA+DEESCDRNL GFLRTI+AT+ LP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRVLP 180

Query: 181 LGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQ 240
            GSASFVV KVSAICPL+LLERVSDLLRWQQK+PC NLPWKLKTFPI S+SSPLYHTL+Q
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRD 300
           PNPLT +E+KSLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHN+D
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLL VTK A++MRVPLGIKLVRGAYMSSE KLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEF 420
           HN+I DTH+CYNSCAS+LL+NIA GSSG ILATHNVESGKLAA KAYEIGI KLK+K+EF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRK 480
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDR+LMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELARRMKDYLR 491
           EL RR+K+YLR
Sbjct: 481 ELGRRVKEYLR 491

BLAST of Lcy09g002910.1 vs. ExPASy TrEMBL
Match: A0A6J1E6Q2 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111431296 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 1.0e-231
Identity = 411/490 (83.88%), Postives = 446/490 (91.02%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAAPPPSLNLAGSPATPSVDFA 60
           MARRI+ATQSK PK+ FLYTF+R LNTAASS AAAFTA AA    LN  GSPATP++DF 
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60

Query: 61  DTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTFY 120
           DT ALFGSL T +LLRAAANLH+AAVGPVVD G+W+M SKLM+VEL RDAILG+VKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120

Query: 121 RHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLPL 180
           RHFCAGEDDAAV +TVRRLH+ GLRSMLDYALEYADDEESCDRNL+GFL+TI+ATKSLP 
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180

Query: 181 GSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQP 240
           GSASFVV KVSAICPL+LLERVSDLLRWQ KNPC NLPWKLKT PI S SSPLYHTL+ P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKHP 240

Query: 241 NPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRDD 300
            PLTPQEEKSLQISHQRL+KICQ+C DANVPLAIDAEHTKVQPAIDYFTYSAAI +N+D 
Sbjct: 241 TPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPIH 360
           NPIVYGTVQAYLKDAKDRLL V KAAD+M++PLGIKLVRGAYMSSE KLASSLGF SPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFPSPIH 360

Query: 361 NNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEFA 420
           + I DTHSCY+ CAS+LL+N+ K SSGVILATHNVESGKLAA+KAYEIGIGKLK K+EFA
Sbjct: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480
           QLYGMSEALSFGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480

Query: 481 LARRMKDYLR 491
           +ARR+KD +R
Sbjct: 481 MARRVKDCMR 490

BLAST of Lcy09g002910.1 vs. ExPASy TrEMBL
Match: A0A5D3BNA9 (Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002050 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.8e-231
Identity = 408/489 (83.44%), Postives = 445/489 (91.00%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAAPPPSLNLAGSPATPSVDFA 60
           MAR +V+TQSKI K+VFLY FNRPLNTAASS AAAFT   APPP L+L   P+TP+VDF 
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFT---APPPPLHL-NPPSTPTVDFT 60

Query: 61  DTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTFY 120
           D+RALFGS+PTSDLL A A LH +A+GPVVDVGMWVM SKLM+VEL RD +LG VKHTFY
Sbjct: 61  DSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTFY 120

Query: 121 RHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLPL 180
           RHFCAGEDD +V +TVRRLH+ GLRSMLDYALEYADDE SCDRNL+GFLRT++ATKSLP 
Sbjct: 121 RHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLPS 180

Query: 181 GSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQP 240
           GSASFVVTKVSAICPL+LLERVSDLLRWQQKNP FNLPWKL+T PI S+SSPLYHTLEQP
Sbjct: 181 GSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQP 240

Query: 241 NPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRDD 300
            PLT +EEKSLQISH+RLMKICQSCVD NVPLAIDAEHTKVQPAIDYFTYSAAIIHN+D 
Sbjct: 241 EPLTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDR 300

Query: 301 NPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPIH 360
           NPIVYGT+QAYLKDAKDRLL   K A  +++PLGIKLVRGAYMSSE KLASSLGFESPIH
Sbjct: 301 NPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIH 360

Query: 361 NNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEFA 420
           N IQDTHSCYN+CAS+LL++IAKGSSG ILATHNVESGKLAA++AYEIGIGKLKQK+EFA
Sbjct: 361 NTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480
           QLYGMSEALSFGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDR+LMRKE
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRKE 480

Query: 481 LARRMKDYL 490
           L RRMK+YL
Sbjct: 481 LGRRMKEYL 485

BLAST of Lcy09g002910.1 vs. ExPASy TrEMBL
Match: A0A6J1JPB7 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111487675 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 7.5e-230
Identity = 410/490 (83.67%), Postives = 445/490 (90.82%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAAPPPSLNLAGSPATPSVDFA 60
           MARRI+ATQSK PK+ FLYTF+R LNTAASS AAAFTA +A P  LNL GSPATP+VDF 
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFTAASASP--LNLTGSPATPTVDFT 60

Query: 61  DTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTFY 120
           DT ALFGSL T +LLRAAANLH+AAVGPVVD G+W+M SKLM+VEL R+AILG+VKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIREAILGSVKHTFY 120

Query: 121 RHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLPL 180
           RHFCAGEDDAAV +TVRRLH+ GLRSMLDYALEYADDEESCDRNL+GFL+TI+A KSLP 
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEAIKSLPP 180

Query: 181 GSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQP 240
           GSASFVV KVSAICPL+LLERVSDLLRWQ KNPC NLPWK KT PI S SSPLYHTL+ P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKFKTLPIFSDSSPLYHTLKHP 240

Query: 241 NPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRDD 300
            PLTP+EEKSLQISHQRLMKICQ+  DANVPLAIDAEHTKVQPAIDYFTYSAAI +N+D 
Sbjct: 241 TPLTPEEEKSLQISHQRLMKICQASADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPIH 360
           NPIVYGTVQAYLKDAKDRLL VTKAAD+M++PLGIKLVRGAYMSSE KLA SLGF SPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSSESKLALSLGFPSPIH 360

Query: 361 NNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEFA 420
           + I DTHSCYN CAS+LL+N+ K SSGVILATHNVESGKLAA+KAYEIGIGKLK K+EFA
Sbjct: 361 DTILDTHSCYNGCASFLLDNVVKSSSGVILATHNVESGKLAAAKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480
           QLYGMSEALSFGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480

Query: 481 LARRMKDYLR 491
           +ARR+KD +R
Sbjct: 481 MARRVKDCMR 488

BLAST of Lcy09g002910.1 vs. ExPASy TrEMBL
Match: A0A6J1JZR8 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.3e-229
Identity = 407/490 (83.06%), Postives = 445/490 (90.82%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAAPPPSLNLAGSPATPSVDFA 60
           MAR I++TQSKIPK VF Y  NR LNT     AAAFT+ A+ PP LNLA SPA P+VDF+
Sbjct: 1   MARSILSTQSKIPKNVFFYASNRLLNT-----AAAFTSAASTPPPLNLAVSPAAPTVDFS 60

Query: 61  DTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTFY 120
           DT+ALFGSL T ++L AAA+LHVAAVGPVVDVGMWVM SKLM+VELARD I G V+H+FY
Sbjct: 61  DTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDIIFGTVRHSFY 120

Query: 121 RHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLPL 180
           RHFCAGEDD AVG+TVRRLHN GLRSMLDYA+EYA+DEESCDRNL GFLRTI+AT++LP 
Sbjct: 121 RHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLRGFLRTIEATRALPP 180

Query: 181 GSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQP 240
           GSASFVV KVSAICPL+LLERVSDLLRWQQK+PC NLPWKLKTFPI S+SSPLYHTL+QP
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQP 240

Query: 241 NPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRDD 300
           NPLT +E+KSLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHN+D 
Sbjct: 241 NPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPIH 360
           NPIVYGT+QAYLKDAKDRLL VTK A+ MRVPLGIKLVRGAYMSSE KLASSLGFESPIH
Sbjct: 301 NPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPIH 360

Query: 361 NNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEFA 420
           N+I DTH+CYNSCAS+LL+NIA GSSG ILATHNVESGKL A KAYEIGI KLK+K+EFA
Sbjct: 361 NSIHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480
           QLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDR+LMRKE
Sbjct: 421 QLYGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMRKE 480

Query: 481 LARRMKDYLR 491
           L RRMK+YLR
Sbjct: 481 LGRRMKEYLR 485

BLAST of Lcy09g002910.1 vs. NCBI nr
Match: KAG6578518.1 (Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 825.9 bits (2132), Expect = 1.9e-235
Identity = 416/491 (84.73%), Postives = 455/491 (92.67%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAA-PPPSLNLAGSPATPSVDF 60
           MAR I++TQSKIPK VF Y FNR LNTAASSSAAAFT+ A+ PPP LNLA SPA P+VDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  ADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTF 120
           +DT+ALFGSL T ++L AAA+LHVAAVGPVVDVGMWVM SKLM+VELARD I G V+H+F
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLP 180
           YRHFCAGEDD AVG+TVRRLHN GLRSMLDYA+EYA+DEESCDRNL GFLRTI+AT++LP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180

Query: 181 LGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQ 240
            GSASFVV KVSAICPL+LLERVSDLLRWQQK+PC NLPWKLKTFPILS+SSPLYHTL+Q
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240

Query: 241 PNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRD 300
           PNPLT +E+KSLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHN+D
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLL VTK A++MRVPLGIKLVRGAYMSSE KLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEF 420
           HN+I DTH+CYNSCAS+LL+NIA GSSG ILATHNVESGKLAA KAYEIGI KLK+K+EF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRK 480
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDR+LMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELARRMKDYLR 491
           EL RRMK+YLR
Sbjct: 481 ELGRRMKEYLR 491

BLAST of Lcy09g002910.1 vs. NCBI nr
Match: XP_022939392.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 821.6 bits (2121), Expect = 3.6e-234
Identity = 414/491 (84.32%), Postives = 453/491 (92.26%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAA-PPPSLNLAGSPATPSVDF 60
           MAR I++TQSKIPK VF Y FNR LNTAASSSAAAFT+ A+ PPP LNLA SPA P+VDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  ADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTF 120
           +DT+ALFGSL T ++L AAA+LHVAAVGPVVDVGMWVM SKLM+VELARD I G V+H+F
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLP 180
           YRHFCAGEDD AVG+TVRRLHN GLRSMLDYA+EYA+DEESCDRNL GFLRTI+AT+ LP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRVLP 180

Query: 181 LGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQ 240
            GSASFVV KVSAICPL+LLERVSDLLRWQQK+PC NLPWKLKTFPI S+SSPLYHTL+Q
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRD 300
           PNPLT +E+KSLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHN+D
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLL VTK A++MRVPLGIKLVRGAYMSSE KLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEF 420
           HN+I DTH+CYNSCAS+LL+NIA GSSG ILATHNVESGKLAA KAYEIGI KLK+K+EF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRK 480
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDR+LMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

Query: 481 ELARRMKDYLR 491
           EL RR+K+YLR
Sbjct: 481 ELGRRVKEYLR 491

BLAST of Lcy09g002910.1 vs. NCBI nr
Match: XP_023551146.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818.9 bits (2114), Expect = 2.3e-233
Identity = 413/492 (83.94%), Postives = 453/492 (92.07%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAA--PPPSLNLAGSPATPSVD 60
           MAR I++TQSKIPK VF Y FNR LNTAASSSAAAFT+ A+  PPP LNLA SPA P+VD
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPPPLNLAVSPAAPTVD 60

Query: 61  FADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHT 120
           F+DT+ALFGSL T ++L AAA+LHVAAVGPVVDVGMWVM SKLM+VELARD I G V+H+
Sbjct: 61  FSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHS 120

Query: 121 FYRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSL 180
           FYRHFCAGEDD AVG+TVRRLH+ GLRSMLDYA+EYA+DEESCDRNL GFLRTI+AT++L
Sbjct: 121 FYRHFCAGEDDTAVGKTVRRLHDVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRAL 180

Query: 181 PLGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLE 240
           P GSASFVV KVSAICPL+LLERVSDLLRWQQK+PC NLPWKLKTFPI S+SSPLYHTL+
Sbjct: 181 PPGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQ 240

Query: 241 QPNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNR 300
           QPNPLT +E+KSLQISHQRLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHN+
Sbjct: 241 QPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK 300

Query: 301 DDNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESP 360
           D NPIVYGT+QAYLKDAKDRLL VTK A+ MRVPLGIKLVRGAYMSSE KLASSLGFESP
Sbjct: 301 DHNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHNNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIE 420
           IHN+I DTH+CYNSCAS+LL+NIA GSSG ILATHNV+SGKLAA KAYEIGI KLK+K+E
Sbjct: 361 IHNSIHDTHACYNSCASFLLDNIAVGSSGAILATHNVDSGKLAAKKAYEIGIEKLKEKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMR 480
           FAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDR+LMR
Sbjct: 421 FAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480

Query: 481 KELARRMKDYLR 491
           KEL RRMK+YLR
Sbjct: 481 KELRRRMKEYLR 492

BLAST of Lcy09g002910.1 vs. NCBI nr
Match: XP_038891036.1 (proline dehydrogenase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 817.0 bits (2109), Expect = 8.8e-233
Identity = 418/491 (85.13%), Postives = 445/491 (90.63%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAAPPP-SLNLAGSPATPSVDF 60
           MAR IV+TQSKIPKKVF Y FNR LNTAASSSAAAFTA A  PP  LNL  SP TP+VDF
Sbjct: 1   MARTIVSTQSKIPKKVFFYVFNRSLNTAASSSAAAFTAAATSPPLPLNL-NSPTTPTVDF 60

Query: 61  ADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTF 120
            DTRALFGSLPTSDLL AA  LH AA+GPVVDVGMWVM SKLM+VEL RD +LG VKHTF
Sbjct: 61  TDTRALFGSLPTSDLLHAATTLHAAAIGPVVDVGMWVMNSKLMDVELVRDLVLGTVKHTF 120

Query: 121 YRHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLP 180
           YR FCAGEDDA V +TVRRLH+ GLRSMLDYALEYADDE SCDRNL+GFLRTI+ TKSLP
Sbjct: 121 YRQFCAGEDDATVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTIEITKSLP 180

Query: 181 LGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQ 240
            GSASFVVTKVSAICPL+LLERVSDLLRWQQKNP FNLPWKLKT PI S+SSPLYHTLEQ
Sbjct: 181 PGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQ 240

Query: 241 PNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRD 300
           P PLT +EEKSLQ+SH+RLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN+D
Sbjct: 241 PKPLTHEEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300

Query: 301 DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPI 360
             PIVYGT+QAYLKDAKDRLL V K A  ++VPLGIKLVRGAYMSSE K+ASSLGFESPI
Sbjct: 301 HIPIVYGTIQAYLKDAKDRLLLVNKEASKLKVPLGIKLVRGAYMSSESKIASSLGFESPI 360

Query: 361 HNNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEF 420
           HN IQDTH CYN+CAS+LL+NIAKGSSGVILATHNVESGKLAA+KAYEIG+GKLKQK+EF
Sbjct: 361 HNTIQDTHVCYNTCASFLLDNIAKGSSGVILATHNVESGKLAATKAYEIGMGKLKQKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRK 480
           AQLYGMSEALSFGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSTSNLDR+LMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMRK 480

Query: 481 ELARRMKDYLR 491
           EL RRMK+ LR
Sbjct: 481 ELGRRMKECLR 490

BLAST of Lcy09g002910.1 vs. NCBI nr
Match: XP_022923652.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 812.4 bits (2097), Expect = 2.2e-231
Identity = 411/490 (83.88%), Postives = 446/490 (91.02%), Query Frame = 0

Query: 1   MARRIVATQSKIPKKVFLYTFNRPLNTAASSSAAAFTAVAAPPPSLNLAGSPATPSVDFA 60
           MARRI+ATQSK PK+ FLYTF+R LNTAASS AAAFTA AA    LN  GSPATP++DF 
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60

Query: 61  DTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVKHTFY 120
           DT ALFGSL T +LLRAAANLH+AAVGPVVD G+W+M SKLM+VEL RDAILG+VKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120

Query: 121 RHFCAGEDDAAVGETVRRLHNAGLRSMLDYALEYADDEESCDRNLEGFLRTIDATKSLPL 180
           RHFCAGEDDAAV +TVRRLH+ GLRSMLDYALEYADDEESCDRNL+GFL+TI+ATKSLP 
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180

Query: 181 GSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYHTLEQP 240
           GSASFVV KVSAICPL+LLERVSDLLRWQ KNPC NLPWKLKT PI S SSPLYHTL+ P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKHP 240

Query: 241 NPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNRDD 300
            PLTPQEEKSLQISHQRL+KICQ+C DANVPLAIDAEHTKVQPAIDYFTYSAAI +N+D 
Sbjct: 241 TPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLGFESPIH 360
           NPIVYGTVQAYLKDAKDRLL V KAAD+M++PLGIKLVRGAYMSSE KLASSLGF SPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFPSPIH 360

Query: 361 NNIQDTHSCYNSCASYLLENIAKGSSGVILATHNVESGKLAASKAYEIGIGKLKQKIEFA 420
           + I DTHSCY+ CAS+LL+N+ K SSGVILATHNVESGKLAA+KAYEIGIGKLK K+EFA
Sbjct: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480
           QLYGMSEALSFGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLMRKE 480

Query: 481 LARRMKDYLR 491
           +ARR+KD +R
Sbjct: 481 MARRVKDCMR 490

BLAST of Lcy09g002910.1 vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 3.0e-146
Identity = 257/432 (59.49%), Postives = 325/432 (75.23%), Query Frame = 0

Query: 57  VDFADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVK 116
           +D +D   LF S+PTSDLLR+ A LH AA+GP+VD+G WVM SKLM+  + R  +LG VK
Sbjct: 63  LDLSDQARLFSSIPTSDLLRSTAVLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVK 122

Query: 117 HTFYRHFCAGEDDAAVGETVRRLHNA-GLRSMLDYALEYADDEESCDRNLEGFLRTIDAT 176
            TFY HFCAGED  A  E VR ++ A GL+ ML Y +E+ADD  SCD N++ F+RTI+A 
Sbjct: 123 STFYDHFCAGEDADAAAERVRSVYEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAA 182

Query: 177 KSLPLGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYH 236
           KSLP    S VV K++AICP+ LL+RVSDLLRW+ K+P F L WKLK+FP+ S+SSPLYH
Sbjct: 183 KSLPTSHFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYH 242

Query: 237 TLEQPNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 296
           T  +P PLT +EE+ L+ +H R+ +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI+
Sbjct: 243 TNSEPEPLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIM 302

Query: 297 HNRD-DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLG 356
            N D D PIVY T+QAYL+DA +RL    + A+   VP+G KLVRGAYMSSE  LA SLG
Sbjct: 303 FNADKDRPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLG 362

Query: 357 FESPIHNNIQDTHSCYNSCASYLLENIAKGSS-GVILATHNVESGKLAASKAYEIGIGKL 416
            +SP+H+ IQDTHSCYN C ++L+E  + GS  GV+LATHN +SG+LA+ KA ++GI K 
Sbjct: 363 CKSPVHDTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQ 422

Query: 417 KQKIEFAQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLD 476
             KIEFAQLYGMS+ALSFGL+ AGF VSKYMPFG V   +PYLLRRA ENRG+++T   D
Sbjct: 423 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHD 482

Query: 477 RKLMRKELARRM 486
           R+LMR EL RR+
Sbjct: 483 RQLMRMELKRRL 494

BLAST of Lcy09g002910.1 vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 504.2 bits (1297), Expect = 1.2e-142
Identity = 249/432 (57.64%), Postives = 319/432 (73.84%), Query Frame = 0

Query: 57  VDFADTRALFGSLPTSDLLRAAANLHVAAVGPVVDVGMWVMKSKLMEVELARDAILGAVK 116
           +D +D   LF S+P S LLR+ A LH  ++GP+VD+G W+M SKLM+  + RD +L  VK
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 117 HTFYRHFCAGEDDAAVGETVRRLH-NAGLRSMLDYALEYADDEESCDRNLEGFLRTIDAT 176
            TFY HFCAGED AA    V  ++ + GL+ ML Y +E+A+D  +CD N++ F+ T++A 
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 177 KSLPLGSASFVVTKVSAICPLKLLERVSDLLRWQQKNPCFNLPWKLKTFPILSQSSPLYH 236
           K+LP    S VV K++AICP+ +L+RVSDLLRWQ KNP F LPWKL +FP+ S  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 237 TLEQPNPLTPQEEKSLQISHQRLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 296
           T  +P PLT +EE+ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI+
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 297 HNRD-DNPIVYGTVQAYLKDAKDRLLAVTKAADDMRVPLGIKLVRGAYMSSECKLASSLG 356
            N D D PIVY T+QAYLKDA +RL    + ++ M VP+G KLVRGAYMSSE KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 357 FESPIHNNIQDTHSCYNSCASYLLENIAKGSS-GVILATHNVESGKLAASKAYEIGIGKL 416
           ++SP+H+ IQ+TH CYN C S+L+E  + GS   VILATHN +SGKL A KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 417 KQKIEFAQLYGMSEALSFGLRNAGFQVSKYMPFGLVDMVMPYLLRRAEENRGLLSTSNLD 476
             KIEFAQLYGMS+ALSFGL+ AGF VSKYMP+G VD  +PYL+RRA ENRG++ST  LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

Query: 477 RKLMRKELARRM 486
           R+LMRKEL RR+
Sbjct: 462 RQLMRKELKRRV 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P929834.2e-14559.49Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 P... [more]
Q6NKX11.6e-14157.64Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 P... [more]
Q86H287.6e-4631.75Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=p... [more]
O452283.4e-3825.59Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh... [more]
Q044999.9e-3826.11Proline dehydrogenase 1, mitochondrial OS=Drosophila melanogaster OX=7227 GN=slg... [more]
Match NameE-valueIdentityDescription
A0A6J1FGN71.7e-23484.32Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1[more]
A0A6J1E6Q21.0e-23183.88Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111431296 PE=3 SV=1[more]
A0A5D3BNA91.8e-23183.44Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16... [more]
A0A6J1JPB77.5e-23083.67Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111487675 PE=3 SV=1[more]
A0A6J1JZR81.3e-22983.06Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6578518.11.9e-23584.73Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022939392.13.6e-23484.32proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata][more]
XP_023551146.12.3e-23383.94proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_038891036.18.8e-23385.13proline dehydrogenase 2, mitochondrial-like [Benincasa hispida][more]
XP_022923652.12.2e-23183.88proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G30775.13.0e-14659.49Methylenetetrahydrofolate reductase family protein [more]
AT5G38710.11.2e-14257.64Methylenetetrahydrofolate reductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002872Proline dehydrogenase domainPFAMPF01619Pro_dhcoord: 134..468
e-value: 4.1E-72
score: 243.0
NoneNo IPR availableGENE3D3.20.20.220coord: 95..488
e-value: 4.3E-91
score: 307.9
NoneNo IPR availablePANTHERPTHR13914:SF25PROLINE DEHYDROGENASEcoord: 1..487
IPR015659Proline oxidase familyPANTHERPTHR13914PROLINE OXIDASEcoord: 1..487
IPR029041FAD-linked oxidoreductase-likeSUPERFAMILY51730FAD-linked oxidoreductasecoord: 126..488

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lcy09g002910Lcy09g002910gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy09g002910.1.exon4Lcy09g002910.1.exon4exon
Lcy09g002910.1.exon3Lcy09g002910.1.exon3exon
Lcy09g002910.1.exon2Lcy09g002910.1.exon2exon
Lcy09g002910.1.exon1Lcy09g002910.1.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy09g002910.1.utr3p1Lcy09g002910.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy09g002910.1.cds4Lcy09g002910.1.cds4CDS
Lcy09g002910.1.cds3Lcy09g002910.1.cds3CDS
Lcy09g002910.1.cds2Lcy09g002910.1.cds2CDS
Lcy09g002910.1.cds1Lcy09g002910.1.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy09g002910.1.utr5p1Lcy09g002910.1.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lcy09g002910.1Lcy09g002910.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006562 proline catabolic process
biological_process GO:0010133 proline catabolic process to glutamate
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004657 proline dehydrogenase activity
molecular_function GO:0071949 FAD binding