Lcy02g012590.1 (mRNA) Sponge gourd (P93075) v1

Overview
NameLcy02g012590.1
TypemRNA
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionmethionine gamma-lyase-like
LocationChr02: 37736338 .. 37738845 (-)
Sequence length1314
RNA-Seq ExpressionLcy02g012590.1
SyntenyLcy02g012590.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGATGTAGGAGTTTACTCCTCTCTTCCGTGTTGGAACAACGATAGCAAGAAATTCAGTTATGATATGAAACCCAAAACTCCATGCATGGAAGGGAAGGAGGATCCTGCAGCTGCTCTGGCGGCAGCCCGCCCCGAGTTTGGAGAGCACGGCGGTGTCAACATGTCGATCGAAAACTCGATAACGTTCACGGTGATGGAGGCCGAAACGATGCGTCGTATGTTCGAAGGGGAGTTGGGCCCAGAACGGGACTTCTTCATCTACAGCCGCCACTTCAACCCCACCGTGATGGCCCTCAGCCGGCAGATGGCGGCGCTAGAAGGAACTGAGGCGGCGTATTGCACAGCCAGTGGGATGGCGGCCGTTTCATCTGTTTTGCTGCAGCTGGTGAGCTCCGGCGGCCATATTGTGGCCTCGAGGACTCTCTACGGCGGCACTCATGCGTTGCTCTCGCATTTTCTGCCGAGGGTCTGCAACATAACGACGACGTTTGTGGATGTGAAGGACCATGAGATGGTTAGAGATGCCATGGTGGAGGGGAAGACCAAGGTAAGTTGGATAATTTTCCATCAACTTCTAAATTTATGTTTGTAACTTACGGTAATTTTTAATTTTAATTTTAATTTTAATTTTAATTTATGTTTTTAATTTTCGTCTCTATAACCATTTCAATTTGATTTCTATAGTTATAGTTTGAGTCGAATTTTACCAATCATATTCATGCTATAATCATATTGATGATCTTTTTAATGACAATTAAGATGATGTTTTTTGTTTGACTAATTGTGTATGTTGACTATATTAAGATTTAATTCCGAATGGGAAAAGAAGAAAAAAAACACGTGAGTTTTTCTAGTGAATGAAATATAATTTAGAAATGATTTGTGAAATTGACACTTTTAAAATTATTTGGAAAGAAATTGAAACTAAACTCAAACAACAGAAACTAAATTGAAGTTTTTAAAATTATGAGGATTACATTTATAATTTAATATTTATTCATATTCTTAACATTTTTATTCCCTTGTTGGTCTTGAATGTTACCACGAAGACCTAACAAGGTAAATATTAATTTGAGAAGAAACAACTTACAAGGGTTTAGTAAACCGTCTCATGCTCTAATGATATGTTAAATTAGTAGTCTAAAATCTTAAGTTTATAGGTTATGATAAATTTAATCTTTTACTCATGTTAGGTGTTGTATTTTGAGACGGTGGCAAACCCGACGCTGATGGTGGCAAATGTGCCGGTGCTTAGTCAGATGGCGCGTGAGAAGGGAGTGACGGTGGTGGTGGATAACACTTTCTTGCCCATGATGATCTCATCGGCAAGGCTCGGTGCTGACGTTGTGGTTCATAGCTGCTCCAAGTTTATCAGCGGCGGCGCCAACGTTATTGCCGGTATGATTAGTGGCTTTCGGATCTAGTTTGTATTGTTAAGTGAGTTAGTTAGAGGGATCTCTGCATGTGCCTTATCCATGATATTTAGTCCAACAATATTTCGGTTTTATGTTTGAGATTTCAAAAACCTTAATTTACAACACTAATATAATTAATCCCATTATTTAATAACCAATACCCAAGAAAATTTTCTAAGTCTAGTTTTATTTCATATTATATATTCACTCTTGTCTATTCTATTTTTTTTGTTTGGAATTAGCTAAATTATAATGCTAATCTCTATGTAGTTTGAACTTGATTTTAATTTGGTTTTTACAATGTTAAAAAATTTCATTATAATCCTTATAGTTTTTTTTTTTTTTTTGGTCCAAAAACAAATCTTCAACAACCAATTGGAAAAAAGAATATTCTGAATAGAAAATCATTTTTACTTATAAAAACATTACTATTCTTTTTCTTTTTAGGCGCTGTTTGTGGGTCAAAAGAATTGGTGAATTCGATGATGGACCTTCACCAAGGCACACTGATGCTTCTTGGGCCAACAATGAACGCCAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAGGCCTCCGAATGAAAGAGCACTCCCACAGAGCTTTAGTCTTTGCTCAAAGAATGAAGAATCTCGGCCTCAAAGTCATATACCCTGGCCTCAAAGACCACCCTGACCACCACCTTCTAACCTCCATAGCAAACCAAGACTATGGCTACGGCGGAGTTTTGTGCATCGACATGGGGACCGAAGACCGAGCCAACAGGCTCATGAATCTCCTCCAAAACCACACCCATTTCGGTTTAATGGCCGTTAGTCTGGGCTTCTTCGAGACCCTCATGTCATGCTCAGGTAATGTTGAATCACCAGGCAACCTAAAAGCTTAAACTAATGAGATATGGTAAATTAATTATTAGTTACTCATATTCTCGACAGGTAGTAGCACGAGTAGTGAGCTGAACGGCGAGGAGAAGGAACTGGCTGGCATTTCTCCAGGATTGATTAGGATGTCTGTTGGATATGTTGGAACCCTAGAACAAAAATGGAGCCAATTGGAGAAGGCACTCTTGAAGATGAGAATTTGA

mRNA sequence

ATGACTGATGTAGGAGTTTACTCCTCTCTTCCGTGTTGGAACAACGATAGCAAGAAATTCAGTTATGATATGAAACCCAAAACTCCATGCATGGAAGGGAAGGAGGATCCTGCAGCTGCTCTGGCGGCAGCCCGCCCCGAGTTTGGAGAGCACGGCGGTGTCAACATGTCGATCGAAAACTCGATAACGTTCACGGTGATGGAGGCCGAAACGATGCGTCGTATGTTCGAAGGGGAGTTGGGCCCAGAACGGGACTTCTTCATCTACAGCCGCCACTTCAACCCCACCGTGATGGCCCTCAGCCGGCAGATGGCGGCGCTAGAAGGAACTGAGGCGGCGTATTGCACAGCCAGTGGGATGGCGGCCGTTTCATCTGTTTTGCTGCAGCTGGTGAGCTCCGGCGGCCATATTGTGGCCTCGAGGACTCTCTACGGCGGCACTCATGCGTTGCTCTCGCATTTTCTGCCGAGGGTCTGCAACATAACGACGACGTTTGTGGATGTGAAGGACCATGAGATGGTTAGAGATGCCATGGTGGAGGGGAAGACCAAGGTGTTGTATTTTGAGACGGTGGCAAACCCGACGCTGATGGTGGCAAATGTGCCGGTGCTTAGTCAGATGGCGCGTGAGAAGGGAGTGACGGTGGTGGTGGATAACACTTTCTTGCCCATGATGATCTCATCGGCAAGGCTCGGTGCTGACGTTGTGGTTCATAGCTGCTCCAAGTTTATCAGCGGCGGCGCCAACGTTATTGCCGGCGCTGTTTGTGGGTCAAAAGAATTGGTGAATTCGATGATGGACCTTCACCAAGGCACACTGATGCTTCTTGGGCCAACAATGAACGCCAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAGGCCTCCGAATGAAAGAGCACTCCCACAGAGCTTTAGTCTTTGCTCAAAGAATGAAGAATCTCGGCCTCAAAGTCATATACCCTGGCCTCAAAGACCACCCTGACCACCACCTTCTAACCTCCATAGCAAACCAAGACTATGGCTACGGCGGAGTTTTGTGCATCGACATGGGGACCGAAGACCGAGCCAACAGGCTCATGAATCTCCTCCAAAACCACACCCATTTCGGTTTAATGGCCGTTAGTCTGGGCTTCTTCGAGACCCTCATGTCATGCTCAGGTAGTAGCACGAGTAGTGAGCTGAACGGCGAGGAGAAGGAACTGGCTGGCATTTCTCCAGGATTGATTAGGATGTCTGTTGGATATGTTGGAACCCTAGAACAAAAATGGAGCCAATTGGAGAAGGCACTCTTGAAGATGAGAATTTGA

Coding sequence (CDS)

ATGACTGATGTAGGAGTTTACTCCTCTCTTCCGTGTTGGAACAACGATAGCAAGAAATTCAGTTATGATATGAAACCCAAAACTCCATGCATGGAAGGGAAGGAGGATCCTGCAGCTGCTCTGGCGGCAGCCCGCCCCGAGTTTGGAGAGCACGGCGGTGTCAACATGTCGATCGAAAACTCGATAACGTTCACGGTGATGGAGGCCGAAACGATGCGTCGTATGTTCGAAGGGGAGTTGGGCCCAGAACGGGACTTCTTCATCTACAGCCGCCACTTCAACCCCACCGTGATGGCCCTCAGCCGGCAGATGGCGGCGCTAGAAGGAACTGAGGCGGCGTATTGCACAGCCAGTGGGATGGCGGCCGTTTCATCTGTTTTGCTGCAGCTGGTGAGCTCCGGCGGCCATATTGTGGCCTCGAGGACTCTCTACGGCGGCACTCATGCGTTGCTCTCGCATTTTCTGCCGAGGGTCTGCAACATAACGACGACGTTTGTGGATGTGAAGGACCATGAGATGGTTAGAGATGCCATGGTGGAGGGGAAGACCAAGGTGTTGTATTTTGAGACGGTGGCAAACCCGACGCTGATGGTGGCAAATGTGCCGGTGCTTAGTCAGATGGCGCGTGAGAAGGGAGTGACGGTGGTGGTGGATAACACTTTCTTGCCCATGATGATCTCATCGGCAAGGCTCGGTGCTGACGTTGTGGTTCATAGCTGCTCCAAGTTTATCAGCGGCGGCGCCAACGTTATTGCCGGCGCTGTTTGTGGGTCAAAAGAATTGGTGAATTCGATGATGGACCTTCACCAAGGCACACTGATGCTTCTTGGGCCAACAATGAACGCCAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAGGCCTCCGAATGAAAGAGCACTCCCACAGAGCTTTAGTCTTTGCTCAAAGAATGAAGAATCTCGGCCTCAAAGTCATATACCCTGGCCTCAAAGACCACCCTGACCACCACCTTCTAACCTCCATAGCAAACCAAGACTATGGCTACGGCGGAGTTTTGTGCATCGACATGGGGACCGAAGACCGAGCCAACAGGCTCATGAATCTCCTCCAAAACCACACCCATTTCGGTTTAATGGCCGTTAGTCTGGGCTTCTTCGAGACCCTCATGTCATGCTCAGGTAGTAGCACGAGTAGTGAGCTGAACGGCGAGGAGAAGGAACTGGCTGGCATTTCTCCAGGATTGATTAGGATGTCTGTTGGATATGTTGGAACCCTAGAACAAAAATGGAGCCAATTGGAGAAGGCACTCTTGAAGATGAGAATTTGA

Protein sequence

MTDVGVYSSLPCWNNDSKKFSYDMKPKTPCMEGKEDPAAALAAARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSKFISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKMRI
Homology
BLAST of Lcy02g012590.1 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 5.0e-174
Identity = 301/400 (75.25%), Postives = 352/400 (88.00%), Query Frame = 0

Query: 36  DPAAALAAARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNP 95
           DPAAA+A  R EFGEHGGVNMSIE S TFTVME +TMRRMF GELGP+ DF++YSRHFNP
Sbjct: 42  DPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHFNP 101

Query: 96  TVMALSRQMAALEGTEAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 155
           TV+ LSRQMAALEGT+AAYCT+SGM+A+SSV+LQL SSGGH+VA+ TLYGGTHALLSHFL
Sbjct: 102 TVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSHFL 161

Query: 156 PRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQMAREKGVTV 215
           PR CNITT+FVD+ DH  V +A+VEG+T+VLYFE+VANPTL VA++P LS+MA EKGVTV
Sbjct: 162 PRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGVTV 221

Query: 216 VVDNTFLPMMISSARLGADVVVHSCSKFISGGANVIAGAVCGSKELVNSMMDLHQGTLML 275
           VVDNTF PM++S A+LGADVVVHS SKFISGGA++IAGAVCGS+ LV  MMDL  G+LML
Sbjct: 222 VVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSLML 281

Query: 276 LGPTMNAKVASELSQRIPHLGLRMKEHSHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLT 335
           LGPTMNAKVA ELS+RIPHLGLRM+EHSHRA V+A+RM++LG+KVIYPGL+ HP H L  
Sbjct: 282 LGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKLFK 341

Query: 336 SIANQDYGYGGVLCIDMGTEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSE 395
            + N+DYGYGG+L IDM TE++AN+LM  LQN T FG MAVSLG++ETLMSCSGSSTSSE
Sbjct: 342 GMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTSSE 401

Query: 396 LNGEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKM 436
           L+  +KE AGISPGL+RMSVGYVGTLEQKW+Q EKA L+M
Sbjct: 402 LDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of Lcy02g012590.1 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.7e-47
Identity = 141/390 (36.15%), Postives = 213/390 (54.62%), Query Frame = 0

Query: 51  HGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGT 110
           +G + M I  + TF    AE   R F      E   +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 111 EAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKD 170
           EAA  T+SGM A+SS L  ++ +G H+V  +TLYG T AL+ H L R   I  TFVD  +
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTRF-GIEVTFVDTSN 141

Query: 171 HEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQMAREKGVT-VVVDNTF-LPMMISS 230
            + V++AM +  T+V+Y ET ANP L + ++  LS++A     T V+VDNTF  P M   
Sbjct: 142 LDEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 231 ARLGADVVVHSCSKFISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASEL 290
            +LGAD+VVHS +K+I+G  +VIAG V  +KEL + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 291 SQRIPHLGLRMKEH---SHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYG 350
            + +    +RM+ H   + + + F  +   +  +V YPGL+ HP H +      Q   +G
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIE-RVYYPGLETHPGHEI---AKKQMKDFG 321

Query: 351 GVLCIDMGTEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAG 410
            ++  ++     A +    L N+     +AVSLG  ETL+    S T S    EE+E AG
Sbjct: 322 AMISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREAAG 381

Query: 411 ISPGLIRMSVGYVGTLEQKWSQLEKALLKM 436
           I+ GL+R+SVG +  +E   + LE+ L K+
Sbjct: 382 ITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of Lcy02g012590.1 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 5.7e-45
Identity = 136/389 (34.96%), Postives = 207/389 (53.21%), Query Frame = 0

Query: 51  HGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGT 110
           +G + M I  + TF    AE   R F      E   +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 111 EAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKD 170
           EA    +SGM A+SS L  ++ +G H+V  +TLYG T AL++H L R   +  TFVD  +
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTRF-GVEVTFVDTSN 141

Query: 171 HEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQMAREKGVT-VVVDNTF-LPMMISS 230
            E V++AM +  T+V+Y ET ANP L + ++  LS++A     T V+VDNTF  P M   
Sbjct: 142 LEEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 231 ARLGADVVVHSCSKFISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASEL 290
            +LG D+VVHS +K+++G  +VIAG V   +EL + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 291 SQRIPHLGLRMKEHSHRALVFAQRMKNLGL--KVIYPGLKDHPDHHLLTSIANQDYGYGG 350
            + +    +RM+ H   A      +       KV YPGL+ HP + +      Q   +G 
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGYEI---AKKQMKDFGA 321

Query: 351 VLCIDMGTEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGI 410
           ++  ++     A +    L N+     +AVSLG  ETL+    S T S    EE+E+AGI
Sbjct: 322 MISFELKGGFEAGK---TLLNNLKLCSLAVSLGDTETLIQHPASMTHSPYTKEEREVAGI 381

Query: 411 SPGLIRMSVGYVGTLEQKWSQLEKALLKM 436
           + GL+R+SVG +  +E   + LE+ L K+
Sbjct: 382 TDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of Lcy02g012590.1 vs. ExPASy Swiss-Prot
Match: P13254 (L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 1.4e-43
Identity = 136/373 (36.46%), Postives = 195/373 (52.28%), Query Frame = 0

Query: 50  EHGGVNM-SIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALE 109
           +HGG  +  +  + TFT    E     F GE         YSR  NPT+  L  +MA+LE
Sbjct: 23  DHGGALVPPVYQTATFTFPTVEYGAACFAGEQAGH----FYSRISNPTLNLLEARMASLE 82

Query: 110 GTEAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDV 169
           G EA    ASGM A++S L  L+  G  ++   TLYG T A L H +     +    VD+
Sbjct: 83  GGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGEF-GVKLRHVDM 142

Query: 170 KDHEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTF-LPMMIS 229
            D + +  AM    T+V+YFE+ ANP + +A++  ++++AR+ G TVVVDNT+  P +  
Sbjct: 143 ADLQALEAAMTPA-TRVIYFESPANPNMHMADIAGVAKIARKHGATVVVDNTYCTPYLQR 202

Query: 230 SARLGADVVVHSCSKFISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASE 289
              LGAD+VVHS +K++SG  ++ AG V GS+ LV+ +    QG   + G  ++   A+ 
Sbjct: 203 PLELGADLVVHSATKYLSGHGDITAGIVVGSQALVDRIR--LQGLKDMTGAVLSPHDAAL 262

Query: 290 LSQRIPHLGLRMKEHSHRALVFAQ---RMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGY 349
           L + I  L LRM  H   A V A+   R   + L + YPGL   P + L     +Q    
Sbjct: 263 LMRGIKTLNLRMDRHCANAQVLAEFLARQPQVEL-IHYPGLASFPQYTLARQQMSQP--- 322

Query: 350 GGVLCIDM-GTEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKEL 409
           GG++  ++ G      R MN LQ  +     AVSLG  E+L     S T S    EE+  
Sbjct: 323 GGMIAFELKGGIGAGRRFMNALQLFSR----AVSLGDAESLAQHPASMTHSSYTPEERAH 379

Query: 410 AGISPGLIRMSVG 417
            GIS GL+R+SVG
Sbjct: 383 YGISEGLVRLSVG 379

BLAST of Lcy02g012590.1 vs. ExPASy Swiss-Prot
Match: O05394 (Cystathionine gamma-lyase OS=Bacillus subtilis (strain 168) OX=224308 GN=mccB PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 4.1e-43
Identity = 125/371 (33.69%), Postives = 203/371 (54.72%), Query Frame = 0

Query: 51  HGGVNMSIE-NSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEG 110
           HGG+    +  +++  + +  T ++   G    +   + YSR  NPT  AL   +  LE 
Sbjct: 9   HGGITGDEKTGAVSVPIYQVSTYKQPKAG----QHTGYEYSRTANPTRTALEALVTELES 68

Query: 111 TEAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVK 170
            EA Y  +SGMAA+++V++ L +SG H+V +  +YGGT+ +++  L R+  I +TFVD  
Sbjct: 69  GEAGYAFSSGMAAITAVMM-LFNSGDHVVLTDDVYGGTYRVMTKVLNRL-GIESTFVDTS 128

Query: 171 DHEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTF-LPMMISS 230
             E V  A +   TK +Y ET  NP L + ++ +++ +A++ GV ++VDNTF  P     
Sbjct: 129 SREEVEKA-IRPNTKAIYIETPTNPLLKITDLTLMADIAKKAGVLLIVDNTFNTPYFQQP 188

Query: 231 ARLGADVVVHSCSKFISGGANVIAG-AVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASE 290
             LGAD+V+HS +K++ G ++V+ G  V  SKEL   +  +   T  +LGP    + +  
Sbjct: 189 LTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGP----QDSWL 248

Query: 291 LSQRIPHLGLRMKEHSHRALVFAQRMKN--LGLKVIYPGLKDHPDHHLLTSIANQDYGYG 350
           L + I  LGLRM+     A   A  ++N      + YPG  +HP H L  +   Q  G+G
Sbjct: 249 LMRGIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKT---QGAGFG 308

Query: 351 GVLCIDMGTEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAG 410
           G++  D+G+E+R +  +  L+  T    +A SLG  E+L+S     T + +  E +   G
Sbjct: 309 GMISFDIGSEERVDAFLGNLKLFT----IAESLGAVESLISVPARMTHASIPRERRLELG 361

Query: 411 ISPGLIRMSVG 417
           I+ GLIR+SVG
Sbjct: 369 ITDGLIRISVG 361

BLAST of Lcy02g012590.1 vs. ExPASy TrEMBL
Match: A0A5A7TIP4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001500 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 392/434 (90.32%), Postives = 407/434 (93.78%), Query Frame = 0

Query: 8   SSLPCWNNDSKKFSYDMKPK-----TPCMEGKEDPAAALAAARPEFGEHGGVNMSIENSI 67
           +SLPC NN+   FSYD KP      T    GK DPAAALA ARPEFGEHGGVNMSIENSI
Sbjct: 11  TSLPCKNNN---FSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHGGVNMSIENSI 70

Query: 68  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAA 127
           TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCT+SGMAA
Sbjct: 71  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAA 130

Query: 128 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGK 187
           VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV++HEMVRDAMVEGK
Sbjct: 131 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGK 190

Query: 188 TKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSK 247
           TKVLYFETVANPTL VAN+P LS+MA EKGVTVVVDNTF PM+IS ARLGADVVVHSCSK
Sbjct: 191 TKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLGADVVVHSCSK 250

Query: 248 FISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 307
           FISGGA++IAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH
Sbjct: 251 FISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 310

Query: 308 SHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLM 367
           S+RALVFAQRMKNLGLKVIYPGL DHPDH LL SIAN+DYGYGGVLCIDMGTE+RANRLM
Sbjct: 311 SNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLM 370

Query: 368 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 427
           NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE
Sbjct: 371 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 430

Query: 428 QKWSQLEKALLKMR 437
           QKWSQLEKALLKM+
Sbjct: 431 QKWSQLEKALLKMK 441

BLAST of Lcy02g012590.1 vs. ExPASy TrEMBL
Match: A0A1S3AU15 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 392/434 (90.32%), Postives = 407/434 (93.78%), Query Frame = 0

Query: 8   SSLPCWNNDSKKFSYDMKPK-----TPCMEGKEDPAAALAAARPEFGEHGGVNMSIENSI 67
           +SLPC NN+   FSYD KP      T    GK DPAAALA ARPEFGEHGGVNMSIENSI
Sbjct: 11  TSLPCKNNN---FSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHGGVNMSIENSI 70

Query: 68  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAA 127
           TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCT+SGMAA
Sbjct: 71  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAA 130

Query: 128 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGK 187
           VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV++HEMVRDAMVEGK
Sbjct: 131 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGK 190

Query: 188 TKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSK 247
           TKVLYFETVANPTL VAN+P LS+MA EKGVTVVVDNTF PM+IS ARLGADVVVHSCSK
Sbjct: 191 TKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLGADVVVHSCSK 250

Query: 248 FISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 307
           FISGGA++IAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH
Sbjct: 251 FISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 310

Query: 308 SHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLM 367
           S+RALVFAQRMKNLGLKVIYPGL DHPDH LL SIAN+DYGYGGVLCIDMGTE+RANRLM
Sbjct: 311 SNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLM 370

Query: 368 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 427
           NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE
Sbjct: 371 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 430

Query: 428 QKWSQLEKALLKMR 437
           QKWSQLEKALLKM+
Sbjct: 431 QKWSQLEKALLKMK 441

BLAST of Lcy02g012590.1 vs. ExPASy TrEMBL
Match: A0A0A0KQL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 3.7e-212
Identity = 385/435 (88.51%), Postives = 406/435 (93.33%), Query Frame = 0

Query: 8   SSLPCWNNDSKKFSYDMKPK-----TPCMEGKEDPAAALAAARPEFGEHGGVNMSIENSI 67
           +SLPC NN++  FSYD +P      +    G+ DPAAALA A PEFGEHGGVNMSIENSI
Sbjct: 10  TSLPCKNNNN--FSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVNMSIENSI 69

Query: 68  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAA 127
           TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCT+SGMAA
Sbjct: 70  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAA 129

Query: 128 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGK 187
           VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV++HEMVRDAMVEGK
Sbjct: 130 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGK 189

Query: 188 TKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSK 247
           TKVLYFETVANPTL VAN+P LS+MA +KGVTVVVDNTF P++IS  RLGADVVVHSCSK
Sbjct: 190 TKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVVVHSCSK 249

Query: 248 FISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 307
           FISGGA++IAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL LRMKEH
Sbjct: 250 FISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEH 309

Query: 308 SHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLM 367
           S+RALVFAQRMKNLGLKVIYPGL DHPDH LL SIAN+DYGYGGVLCIDMGTE+RANRLM
Sbjct: 310 SNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLM 369

Query: 368 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 427
           NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE
Sbjct: 370 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 429

Query: 428 QKWSQLEKALLKMRI 438
           QKW+QLEKALLKM+I
Sbjct: 430 QKWNQLEKALLKMKI 442

BLAST of Lcy02g012590.1 vs. ExPASy TrEMBL
Match: A0A6J1I2P5 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 8.8e-206
Identity = 368/429 (85.78%), Postives = 396/429 (92.31%), Query Frame = 0

Query: 13  WNNDSKKFSYDMK----PKTPCMEGKEDPAAALAAARPEFGEHGGVNMSIENSITFTVME 72
           W+  +KK     +    P T   E +EDPAA+LAA RPEFGEHGGVN+SIENSI+FTVME
Sbjct: 3   WDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVME 62

Query: 73  AETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAVSSVLL 132
           AETMR+MFEGELGPE+D FIYSRHFNPTVMAL RQMAALEGTEAAYCT+SGMAAVSSVLL
Sbjct: 63  AETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLL 122

Query: 133 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYF 192
           QL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVDVKDH MVRDAMV+GKTKVLYF
Sbjct: 123 QLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYF 182

Query: 193 ETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSKFISGGA 252
           ETVANPTL VAN+P LS+MA EKGVTVVVDNTF PM+IS  RLGADVVVHSCSKFISGGA
Sbjct: 183 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 242

Query: 253 NVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSHRALV 312
           ++IAGAVCG K+LVNSMMDLHQGTLMLLGPTMNAKVASELSQR+PHL LRMKEHSHR LV
Sbjct: 243 DIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLV 302

Query: 313 FAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLMNLLQNH 372
           FAQRMKNLGLKVIYPGLKDHPDHHLL+SIAN+DYGYGGVLCIDMGTE+RANRLMNLLQNH
Sbjct: 303 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNH 362

Query: 373 THFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQL 432
           THFG +AVSLG+FETL+SCSGSSTSSE+N EEKELAGISPGLIRMSV YVGTLEQKWSQL
Sbjct: 363 THFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQL 422

Query: 433 EKALLKMRI 438
           EKALLK+ I
Sbjct: 423 EKALLKLNI 431

BLAST of Lcy02g012590.1 vs. ExPASy TrEMBL
Match: A0A6J1E0P6 (methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 5.2e-198
Identity = 360/433 (83.14%), Postives = 387/433 (89.38%), Query Frame = 0

Query: 11  PCWNNDSKKFSYDMKPKTPCMEGK----EDPAAALAAARPEFGEHGGVNMSIENSITFTV 70
           PCWN   K   Y+ K K+P    K    EDPAAALA A  EFGEHGGVNMS+E SITFTV
Sbjct: 12  PCWNEKKKLIKYE-KAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTV 71

Query: 71  MEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAVSSV 130
           MEAETMRRMFEGE GPE + FIYSRHFNPTVMALSRQMAALEGTEAAYCT+SGMAAVSSV
Sbjct: 72  MEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSV 131

Query: 131 LLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVL 190
           LLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVD+KDH MV +AM+EGKTKVL
Sbjct: 132 LLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVL 191

Query: 191 YFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSKFISG 250
           YFETVANPTL VA++P LS+MA EKGVTVVVDNTF PM+IS  RLGADVVVHS SKFISG
Sbjct: 192 YFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISG 251

Query: 251 GANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSHRA 310
           GA++IAGAVCGSK+LV+SMMDL QGTLMLLGPTMN KVA ELSQR+PHLGLRMKEH HRA
Sbjct: 252 GADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRA 311

Query: 311 LVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQD--YGYGGVLCIDMGTEDRANRLMNL 370
           L+FAQR++NLG KVIYPGLKDHPDHHL+ SIAN    YGYGGVLCIDM TE+RANRLMNL
Sbjct: 312 LIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNL 371

Query: 371 LQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQK 430
           LQNHT FGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGL+R+SVGYVGTLEQK
Sbjct: 372 LQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQK 431

Query: 431 WSQLEKALLKMRI 438
           WSQLE+ LLKM++
Sbjct: 432 WSQLERGLLKMKL 443

BLAST of Lcy02g012590.1 vs. NCBI nr
Match: XP_038875936.1 (methionine gamma-lyase-like [Benincasa hispida])

HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 393/434 (90.55%), Postives = 408/434 (94.01%), Query Frame = 0

Query: 8   SSLPCWNNDSKKFSYDMKPKTPCME----GKEDPAAALAAARPEFGEHGGVNMSIENSIT 67
           +SLPCWN D+KKFSY  K  TP +     G EDPAA LA ARPEFGEHGGVNMSIENSIT
Sbjct: 10  TSLPCWNIDNKKFSYQTKSATPMVTSWPMGDEDPAATLAMARPEFGEHGGVNMSIENSIT 69

Query: 68  FTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAV 127
           FTVMEAETMR MFEGELGPERDFFIYSRHFNPTV+ALSRQMAALEGTEAAYCT+SGMAAV
Sbjct: 70  FTVMEAETMRMMFEGELGPERDFFIYSRHFNPTVLALSRQMAALEGTEAAYCTSSGMAAV 129

Query: 128 SSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKT 187
           SSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKT
Sbjct: 130 SSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKT 189

Query: 188 KVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSKF 247
           KVLYFETVANPTL VANVP LS+MA EKGVTVVVDNTF PM+IS ARLGADVVVHSCSKF
Sbjct: 190 KVLYFETVANPTLTVANVPELSRMAHEKGVTVVVDNTFSPMIISPARLGADVVVHSCSKF 249

Query: 248 ISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHS 307
           ISGGA++IAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASE+SQRIPHLGLRMKEHS
Sbjct: 250 ISGGADIIAGAVCGSKNLVNSMMDLHQGTLMLLGPTMNAKVASEISQRIPHLGLRMKEHS 309

Query: 308 HRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLMN 367
           HRALVFAQRMKNLGLKV YPGL DHPDH LL+ IAN+DYGYGGVLCIDMGTE+RANRLMN
Sbjct: 310 HRALVFAQRMKNLGLKVTYPGLNDHPDHQLLSYIANKDYGYGGVLCIDMGTEERANRLMN 369

Query: 368 LLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQ 427
           LLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELN +EKELAGISPGLIRMSVGYVGTLEQ
Sbjct: 370 LLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNSKEKELAGISPGLIRMSVGYVGTLEQ 429

Query: 428 KWSQLEKALLKMRI 438
           KWSQLEKALLKM+I
Sbjct: 430 KWSQLEKALLKMKI 443

BLAST of Lcy02g012590.1 vs. NCBI nr
Match: XP_008437446.1 (PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine gamma-lyase-like [Cucumis melo var. makuwa] >TYK06049.1 methionine gamma-lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 758.8 bits (1958), Expect = 2.5e-215
Identity = 392/434 (90.32%), Postives = 407/434 (93.78%), Query Frame = 0

Query: 8   SSLPCWNNDSKKFSYDMKPK-----TPCMEGKEDPAAALAAARPEFGEHGGVNMSIENSI 67
           +SLPC NN+   FSYD KP      T    GK DPAAALA ARPEFGEHGGVNMSIENSI
Sbjct: 11  TSLPCKNNN---FSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHGGVNMSIENSI 70

Query: 68  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAA 127
           TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCT+SGMAA
Sbjct: 71  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAA 130

Query: 128 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGK 187
           VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV++HEMVRDAMVEGK
Sbjct: 131 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGK 190

Query: 188 TKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSK 247
           TKVLYFETVANPTL VAN+P LS+MA EKGVTVVVDNTF PM+IS ARLGADVVVHSCSK
Sbjct: 191 TKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLGADVVVHSCSK 250

Query: 248 FISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 307
           FISGGA++IAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH
Sbjct: 251 FISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 310

Query: 308 SHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLM 367
           S+RALVFAQRMKNLGLKVIYPGL DHPDH LL SIAN+DYGYGGVLCIDMGTE+RANRLM
Sbjct: 311 SNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLM 370

Query: 368 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 427
           NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE
Sbjct: 371 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 430

Query: 428 QKWSQLEKALLKMR 437
           QKWSQLEKALLKM+
Sbjct: 431 QKWSQLEKALLKMK 441

BLAST of Lcy02g012590.1 vs. NCBI nr
Match: XP_004143943.3 (methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_000014 [Cucumis sativus])

HSP 1 Score: 747.3 bits (1928), Expect = 7.6e-212
Identity = 385/435 (88.51%), Postives = 406/435 (93.33%), Query Frame = 0

Query: 8   SSLPCWNNDSKKFSYDMKPK-----TPCMEGKEDPAAALAAARPEFGEHGGVNMSIENSI 67
           +SLPC NN++  FSYD +P      +    G+ DPAAALA A PEFGEHGGVNMSIENSI
Sbjct: 10  TSLPCKNNNN--FSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVNMSIENSI 69

Query: 68  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAA 127
           TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCT+SGMAA
Sbjct: 70  TFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAA 129

Query: 128 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGK 187
           VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV++HEMVRDAMVEGK
Sbjct: 130 VSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGK 189

Query: 188 TKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSK 247
           TKVLYFETVANPTL VAN+P LS+MA +KGVTVVVDNTF P++IS  RLGADVVVHSCSK
Sbjct: 190 TKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVVVHSCSK 249

Query: 248 FISGGANVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEH 307
           FISGGA++IAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL LRMKEH
Sbjct: 250 FISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEH 309

Query: 308 SHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLM 367
           S+RALVFAQRMKNLGLKVIYPGL DHPDH LL SIAN+DYGYGGVLCIDMGTE+RANRLM
Sbjct: 310 SNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLM 369

Query: 368 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 427
           NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE
Sbjct: 370 NLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLE 429

Query: 428 QKWSQLEKALLKMRI 438
           QKW+QLEKALLKM+I
Sbjct: 430 QKWNQLEKALLKMKI 442

BLAST of Lcy02g012590.1 vs. NCBI nr
Match: KAG7017043.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 743.0 bits (1917), Expect = 1.4e-210
Identity = 374/429 (87.18%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 13  WNNDSKKFSYDMKPKTPCM----EGKEDPAAALAAARPEFGEHGGVNMSIENSITFTVME 72
           W+  +K+  +   P  P +    EG+EDPAA+LAAARPEFGEHGGVN+SIENSITFTVME
Sbjct: 8   WDVKNKRLKHTAPPPPPPVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVME 67

Query: 73  AETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAVSSVLL 132
           AETMR+MFEGELGPE+DFFIYSRHFNPTVMAL RQMAA+EGTEAAYCT+SGMAA+SSVLL
Sbjct: 68  AETMRKMFEGELGPEQDFFIYSRHFNPTVMALGRQMAAIEGTEAAYCTSSGMAAISSVLL 127

Query: 133 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYF 192
           QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVDVKDH  VRDAMV+ KTKVLYF
Sbjct: 128 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAKVRDAMVKDKTKVLYF 187

Query: 193 ETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSKFISGGA 252
           ETVANPTL VAN+P LS+MA EKGVTVVVDNTF PM+IS  RLGADVVVHSCSKFISGGA
Sbjct: 188 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 247

Query: 253 NVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSHRALV 312
           ++IAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQR+PHL LRMKEHSHRALV
Sbjct: 248 DIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALV 307

Query: 313 FAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLMNLLQNH 372
           FAQRMKNLGLKVIYPGLKDHPDHHLL+SIAN+DYGYGGVLCIDMGTE+RANRLMNLLQNH
Sbjct: 308 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNH 367

Query: 373 THFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQL 432
           THFGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGLIRMSVGYVGTLEQKWSQL
Sbjct: 368 THFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWSQL 427

Query: 433 EKALLKMRI 438
           EKALLKM I
Sbjct: 428 EKALLKMNI 436

BLAST of Lcy02g012590.1 vs. NCBI nr
Match: XP_023520565.1 (methionine gamma-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739.6 bits (1908), Expect = 1.6e-209
Identity = 376/429 (87.65%), Postives = 400/429 (93.24%), Query Frame = 0

Query: 13  WNNDSKKFSYDMKPKTPCM----EGKEDPAAALAAARPEFGEHGGVNMSIENSITFTVME 72
           W + +KK      P  P +    EG+EDPAA+LAAARPEFGEHGGVN+SIENSITFTVME
Sbjct: 7   WWDKNKKLKPTAPPPPPTVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVME 66

Query: 73  AETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAVSSVLL 132
           AE MR+MFEGELG E+DFFIYSRHFNPTVMAL R MAALEGTEAAYCT+SGMAAVSSVLL
Sbjct: 67  AEMMRKMFEGELGLEQDFFIYSRHFNPTVMALGRAMAALEGTEAAYCTSSGMAAVSSVLL 126

Query: 133 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYF 192
           QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVDVKDH MVRDAMV+GKTKVLYF
Sbjct: 127 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYF 186

Query: 193 ETVANPTLMVANVPVLSQMAREKGVTVVVDNTFLPMMISSARLGADVVVHSCSKFISGGA 252
           ETVANPTL VANVP LS+MA EKGVTVVVDNTF PM+IS  RLGADVVVHSCSKFISGGA
Sbjct: 187 ETVANPTLTVANVPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 246

Query: 253 NVIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSHRALV 312
           ++IAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQR+PHL LRMKEHSHRALV
Sbjct: 247 DIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALV 306

Query: 313 FAQRMKNLGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMGTEDRANRLMNLLQNH 372
           FAQRMKNLGLKVIYPGLKDHPDHHLL+SIAN+DYGYGGVLCIDMGTE+RANRLMNLLQNH
Sbjct: 307 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNH 366

Query: 373 THFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQL 432
           THFGL+AVSLG+FETLMSCSGSSTSSE+N EEKELAGISPGLIRMSVGYVGTLEQKWSQL
Sbjct: 367 THFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWSQL 426

Query: 433 EKALLKMRI 438
           EKALLK+ I
Sbjct: 427 EKALLKLNI 435

BLAST of Lcy02g012590.1 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 612.1 bits (1577), Expect = 3.6e-175
Identity = 301/400 (75.25%), Postives = 352/400 (88.00%), Query Frame = 0

Query: 36  DPAAALAAARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNP 95
           DPAAA+A  R EFGEHGGVNMSIE S TFTVME +TMRRMF GELGP+ DF++YSRHFNP
Sbjct: 42  DPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHFNP 101

Query: 96  TVMALSRQMAALEGTEAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFL 155
           TV+ LSRQMAALEGT+AAYCT+SGM+A+SSV+LQL SSGGH+VA+ TLYGGTHALLSHFL
Sbjct: 102 TVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSHFL 161

Query: 156 PRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQMAREKGVTV 215
           PR CNITT+FVD+ DH  V +A+VEG+T+VLYFE+VANPTL VA++P LS+MA EKGVTV
Sbjct: 162 PRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGVTV 221

Query: 216 VVDNTFLPMMISSARLGADVVVHSCSKFISGGANVIAGAVCGSKELVNSMMDLHQGTLML 275
           VVDNTF PM++S A+LGADVVVHS SKFISGGA++IAGAVCGS+ LV  MMDL  G+LML
Sbjct: 222 VVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSLML 281

Query: 276 LGPTMNAKVASELSQRIPHLGLRMKEHSHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLT 335
           LGPTMNAKVA ELS+RIPHLGLRM+EHSHRA V+A+RM++LG+KVIYPGL+ HP H L  
Sbjct: 282 LGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKLFK 341

Query: 336 SIANQDYGYGGVLCIDMGTEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSE 395
            + N+DYGYGG+L IDM TE++AN+LM  LQN T FG MAVSLG++ETLMSCSGSSTSSE
Sbjct: 342 GMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTSSE 401

Query: 396 LNGEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKM 436
           L+  +KE AGISPGL+RMSVGYVGTLEQKW+Q EKA L+M
Sbjct: 402 LDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of Lcy02g012590.1 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 2.8e-39
Identity = 110/334 (32.93%), Postives = 181/334 (54.19%), Query Frame = 0

Query: 87  FIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLYGG 146
           F Y R+ NPT + L  +++ALEG E+    ASGM A + +LL LV +GGHIV +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 147 THALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYFETVANPTLMVANVPVLSQ 206
           T   + +FLP++  IT T +D  D   +  A+ E K  + + E+  NP L   ++ ++S+
Sbjct: 284 TRIFMENFLPKL-GITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 207 MAREKGVTVVVDNTF-LPMMISSARLGADVVVHSCSKFISGGANVIAGAVCGSKELVNSM 266
           +  ++G  V +D TF  P+   +  LGAD+VVHS +K+I G  +V+AG +CGS +LV+ +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 267 MDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSHRALVFAQRMK--NLGLKVIYP 326
            +LH     +LG T+N   A  + + +  L LR+++ +  A   A+ ++       V YP
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVSHVYYP 463

Query: 327 GLKDHPDHHLLTSIANQDYGYGGVLCIDM-GTEDRANRLMNLLQNHTHFGLMAVSLGFFE 386
           GL  HP+H L      Q  G+GGV+  ++ G  +   + ++ L+       +A S G  E
Sbjct: 464 GLPSHPEHEL---AKRQMTGFGGVVSFEIDGDIETTIKFVDSLK----IPYIAPSFGGCE 523

Query: 387 TLMSCSGSSTSSELNGEEKELAGISPGLIRMSVG 417
           +++      +  +L  EE+   GI   L+R S G
Sbjct: 524 SIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFG 545

BLAST of Lcy02g012590.1 vs. TAIR 10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 3.0e-33
Identity = 102/336 (30.36%), Postives = 177/336 (52.68%), Query Frame = 0

Query: 85  DFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASGMAAVSSVLLQLVSSGGHIVASRTLY 144
           D + Y+R+ NPT MAL  +++ LEG E+    ASGM A + +LL LV + GHIVA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 145 GGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVLYFETVANPTLMVANVPVL 204
             T   + +FL ++  IT TF+D  D   ++  +   +  + + E+  NP L   ++ ++
Sbjct: 132 KETRIFMENFLTKL-GITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLV 191

Query: 205 SQMAREKGVTVVVDNTF-LPMMISSARLGADVVVHSCSKFISGGANVIAGAVCGSKELVN 264
           S++   +G  V +D T   P+   +  LGAD+V HS +K+I G  + +AG++ GS ELV+
Sbjct: 192 SKICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVS 251

Query: 265 SMMDLHQGTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSHRALVFAQRMK--NLGLKVI 324
            + +LH+    LLG T+N   A  L + +  + LR+++ +   +  AQ ++      +V 
Sbjct: 252 KIRNLHK----LLGGTLNPNAAYLLIRGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVY 311

Query: 325 YPGLKDHPDHHLLTSIANQDYGYGGVLCIDM-GTEDRANRLMNLLQNHTHFGLMAVSLGF 384
           Y GL  HP+H +      Q  G GG++  ++ G      + ++ L+       +A S G 
Sbjct: 312 YLGLPSHPEHLI---AKRQMTGIGGLISFEIDGDLKTTIKFIDALK----IPYLAASFGG 371

Query: 385 FETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVG 417
            E+L+    +    ++  EE+   G    L+R S G
Sbjct: 372 CESLVDQLATGI-WDIPREERLKDGFQDNLVRFSFG 394

BLAST of Lcy02g012590.1 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 132.9 bits (333), Expect = 6.3e-31
Identity = 127/423 (30.02%), Postives = 202/423 (47.75%), Query Frame = 0

Query: 8   SSLPCW------NNDSKKFSYDMKPKTPCMEGKEDPAAA--LAAARPEFGEHGGVNMSIE 67
           S +P W      N +S K +  M+     M  KE+ + +  L     +F     ++  + 
Sbjct: 35  SKVPTWEKKQISNRNSFKLNCVMEKSVDAMNIKEEASVSTLLVNLDNKFDPFDAMSTPLY 94

Query: 68  NSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASG 127
            + TF    A         E GP    + Y+R  NPT  AL   +A L+  + A+C  SG
Sbjct: 95  QTATFKQPSAI--------ENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 154

Query: 128 MAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMV 187
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR   +       K  E+   A +
Sbjct: 155 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVA--AAI 214

Query: 188 EGKTKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFL-PMMISSARLGADVVVH 247
             +TK+++ E+  NP   ++++  +S+MA  +G  V+VDN+ + P++     LGAD+V+H
Sbjct: 215 GPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMH 274

Query: 248 SCSKFISGGANVIAG--AVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQR-IPHL 307
           S +KFI+G ++V+AG  AV G K L   +  L       L P         L  R I  +
Sbjct: 275 SATKFIAGHSDVMAGVLAVKGEK-LAKEVYFLQNSEGSGLAP-----FDCWLCLRGIKTM 334

Query: 308 GLRMKEHSHRALVFAQRMKN--LGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMG 367
            LR+++    A   A  + +     KV Y GL DHP HHL  S   Q  G G V     G
Sbjct: 335 ALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFS---QAKGAGSVFSFITG 394

Query: 368 TEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 417
           +   +  L+      T +  +AVS G  ++L+S     + + +  E +E  G++  L+R+
Sbjct: 395 SVALSKHLVET----TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRI 429

BLAST of Lcy02g012590.1 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-30
Identity = 113/363 (31.13%), Postives = 184/363 (50.69%), Query Frame = 0

Query: 60  NSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTASG 119
           ++++  + +  T ++    E GP    + Y+R  NPT  AL   +A L+  + A+C  SG
Sbjct: 102 DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 161

Query: 120 MAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMV 179
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR   +       K  E+   A +
Sbjct: 162 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVA--AAI 221

Query: 180 EGKTKVLYFETVANPTLMVANVPVLSQMAREKGVTVVVDNTFL-PMMISSARLGADVVVH 239
             +TK+++ E+  NP   ++++  +S+MA  +G  V+VDN+ + P++     LGAD+V+H
Sbjct: 222 GPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMH 281

Query: 240 SCSKFISGGANVIAG--AVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQR-IPHL 299
           S +KFI+G ++V+AG  AV G K L   +  L       L P         L  R I  +
Sbjct: 282 SATKFIAGHSDVMAGVLAVKGEK-LAKEVYFLQNSEGSGLAP-----FDCWLCLRGIKTM 341

Query: 300 GLRMKEHSHRALVFAQRMKN--LGLKVIYPGLKDHPDHHLLTSIANQDYGYGGVLCIDMG 359
            LR+++    A   A  + +     KV Y GL DHP HHL  S   Q  G G V     G
Sbjct: 342 ALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFS---QAKGAGSVFSFITG 401

Query: 360 TEDRANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 417
           +   +  L+      T +  +AVS G  ++L+S     + + +  E +E  G++  L+R+
Sbjct: 402 SVALSKHLVET----TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRI 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SGU95.0e-17475.25Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1[more]
Q8L0X44.7e-4736.15L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ... [more]
Q8RDT45.7e-4534.96L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC... [more]
P132541.4e-4336.46L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2[more]
O053944.1e-4333.69Cystathionine gamma-lyase OS=Bacillus subtilis (strain 168) OX=224308 GN=mccB PE... [more]
Match NameE-valueIdentityDescription
A0A5A7TIP41.2e-21590.32Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AU151.2e-21590.32methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1[more]
A0A0A0KQL73.7e-21288.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1[more]
A0A6J1I2P58.8e-20685.78methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=... [more]
A0A6J1E0P65.2e-19883.14methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038875936.11.7e-21990.55methionine gamma-lyase-like [Benincasa hispida][more]
XP_008437446.12.5e-21590.32PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine g... [more]
XP_004143943.37.6e-21288.51methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_00... [more]
KAG7017043.11.4e-21087.18Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023520565.11.6e-20987.65methionine gamma-lyase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G64660.13.6e-17575.25methionine gamma-lyase [more]
AT3G01120.12.8e-3932.93Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT1G33320.13.0e-3330.36Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT3G57050.26.3e-3130.02cystathionine beta-lyase [more]
AT3G57050.11.1e-3031.13cystathionine beta-lyase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePFAMPF01053Cys_Met_Meta_PPcoord: 50..416
e-value: 3.6E-80
score: 269.3
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePIRSFPIRSF001434CGScoord: 23..436
e-value: 2.9E-98
score: 327.1
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePANTHERPTHR11808TRANS-SULFURATION ENZYME FAMILY MEMBERcoord: 24..435
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 38..291
e-value: 1.8E-63
score: 216.1
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 296..436
e-value: 3.4E-24
score: 87.5
NoneNo IPR availablePIRSRPIRSR001434-2PIRSR001434-2coord: 90..396
e-value: 3.1E-75
score: 251.0
NoneNo IPR availablePANTHERPTHR11808:SF76METHIONINE GAMMA-LYASE-LIKEcoord: 24..435
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 67..422

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lcy02g012590Lcy02g012590gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy02g012590.1.exon4Lcy02g012590.1.exon4exon
Lcy02g012590.1.exon3Lcy02g012590.1.exon3exon
Lcy02g012590.1.exon2Lcy02g012590.1.exon2exon
Lcy02g012590.1.exon1Lcy02g012590.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy02g012590.1.cds4Lcy02g012590.1.cds4CDS
Lcy02g012590.1.cds3Lcy02g012590.1.cds3CDS
Lcy02g012590.1.cds2Lcy02g012590.1.cds2CDS
Lcy02g012590.1.cds1Lcy02g012590.1.cds1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lcy02g012590.1Lcy02g012590.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019346 transsulfuration
biological_process GO:0019343 cysteine biosynthetic process via cystathionine
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003824 catalytic activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004123 cystathionine gamma-lyase activity
molecular_function GO:0018826 methionine gamma-lyase activity