Lag0041333.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0041333.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr13: 15841175 .. 15843013 (+)
Sequence length1839
RNA-Seq ExpressionLag0041333.1
SyntenyLag0041333.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGGAGAATTTTTCAAAAACTTTTGGAACATTTTGAAGCCCAACTTAGTAGAGGTGTTCCACGAGTTTTTTAAAAATGGTATCATCAATAAAAGAACAAACGAGACTTACATCTGCCTGATCCCTAAAAAGAGGAAGGCCTCAAATGTGAGAGATTTTCGACCTATTAGTCTGGTTACCTCCTTATACAAAATTATTGCAAAAGTCCTTGTTGAGAGGCTAAAGAAGGTCCTCCCTCAGACGATTAGTGATTGTCAAGTTGCTTTCGTTCACGGAAGGCAAATTTTAGATCCGATTCTTGTGGCCAACGAAACAGTGGAAGATTACAGGCTCGAAAAGAAAGAGGGTATATTGATGAAACTTGATCTGGAAAAAGCCTACGACAAAGTTAGTTGGAAGTTCCTCGATGCCATTCTAGCTTTAAAAGGTTTTGGTAAAACTTGGAGAATGTGGATCCAGAGGTGTCTTTTGAACACCAACTTCTCGGTGATCATCAATGGGAGACCTAGGGGGAAAATTCAAGCCTCTAGAGGACTTAGGCAAGGGGATCCCCTCTCCCCATTCCTTTTCACGATTGTGGGCGATGCCCTGAGCCGATCTGTTCAATATTGCATCGAGAAAAACATCCTAAAAGGCCTTGTTGTGGACAGAAACAGCATTGAGGTTACCATGCTCCAATACGCTGACGACACATTGATCTTCTGCCCAATTGTGAATGAGATTTTCGACAATTGGTGGGAGATTTTGAATATGATCCTAGAAGGGGCGGGGCTTAACTTGAATCTTGCAAAAACCTCCATCATAGGCATCGCCTCCATTATAGGCATCAATATCAATGAGGATACGATTAGGAATAAGGTGGTTAAAATGGGTTGTAGAGCTGAAAAGTTCCCGATAAATTACCTTGGCTTCCCCCTTGGGGGTAATCATCATACAGTTGCGTTTTGGGATCCTTTGCTGGACAAGTTCAACGCTAAGCTGGACGCGTGGAGGAAGCTCCTCCTTTCTAAGGGTGGTAGAGTAACCTTGGCTCAATCCATCCTTAATAGCCTCCCAATTTATCACTTCTCCTTGCTAAAGGCTCCTAAGAAGGTGATAGATCGGATGGAGAAATTAATTAGAGATTTTGTTTGGAATGGTGGTACTTTCAAACCAGGTAGTAATCTTGTAAAATGGGAATGGACAGCTTTGCCAACATATTGTGGGGGCTTGGGAGTGGGGTCTCTAGCCAATTGGAACGTGGCCCTCCTTACTAAATGGTTATGGAGATTCACCCAAGAAGAAAGCGCCCTATGGAGAAAGGTGATTGCTAGCATTTACGGTGTCACGGCCATGGGATGGATGACTGTGCATTCGGCCAAGAAAATCGGGAGGAGATTCTGGCTCGACATCAACAAGTACACCTCCCAATTTACTAAATTTGCTAAATTCCAGGTTAATTGTGGCCAAAGAATCAGATTTTGGGATGATATTTGGTGTGATACTCGCCCTCTCAGTGTTGCTTTCCCAGACATCTATACTATCTCTCAAAAGAAAAATGCCTCCATTTATGAGTGTTGGGATGCAAACTCTCAAGCTTGGAATTTAGGCTTGCGAAGAGGCCTTTTTGACCGAGAAACCAGCAGTTGGGTGGCGCTAGTTGATAAGCTAAGTCTGGTGCAGCTGGGAGATGGTCCTGATAGAATTATTTGGAACTTGGATAATTCAGGGAGCTTTACCACTAATTCTCTGTTTAGAAATCAGATTGGGCATTCTTCTAAAGTCAACCACTCCCTGGCAGAGCTGATATGGAGGCACAAATGCCCCAAAAAAGTTAAAAAAAATTGTGGTCAGTAG

mRNA sequence

ATGACTGGAGAATTTTTCAAAAACTTTTGGAACATTTTGAAGCCCAACTTAGTAGAGGTGTTCCACGAGTTTTTTAAAAATGGTATCATCAATAAAAGAACAAACGAGACTTACATCTGCCTGATCCCTAAAAAGAGGAAGGCCTCAAATGTGAGAGATTTTCGACCTATTAGTCTGGTTACCTCCTTATACAAAATTATTGCAAAAGTCCTTGTTGAGAGGCTAAAGAAGGTCCTCCCTCAGACGATTAGTGATTGTCAAGTTGCTTTCGTTCACGGAAGGCAAATTTTAGATCCGATTCTTGTGGCCAACGAAACAGTGGAAGATTACAGGCTCGAAAAGAAAGAGGGTATATTGATGAAACTTGATCTGGAAAAAGCCTACGACAAAGTTAGTTGGAAGTTCCTCGATGCCATTCTAGCTTTAAAAGGTTTTGGTAAAACTTGGAGAATGTGGATCCAGAGGTGTCTTTTGAACACCAACTTCTCGGTGATCATCAATGGGAGACCTAGGGGGAAAATTCAAGCCTCTAGAGGACTTAGGCAAGGGGATCCCCTCTCCCCATTCCTTTTCACGATTGTGGGCGATGCCCTGAGCCGATCTGTTCAATATTGCATCGAGAAAAACATCCTAAAAGGCCTTGTTGTGGACAGAAACAGCATTGAGGTTACCATGCTCCAATACGCTGACGACACATTGATCTTCTGCCCAATTGTGAATGAGATTTTCGACAATTGGTGGGAGATTTTGAATATGATCCTAGAAGGGGCGGGGCTTAACTTGAATCTTGCAAAAACCTCCATCATAGGCATCGCCTCCATTATAGGCATCAATATCAATGAGGATACGATTAGGAATAAGGTGGTTAAAATGGGTTGTAGAGCTGAAAAGTTCCCGATAAATTACCTTGGCTTCCCCCTTGGGGGTAATCATCATACAGTTGCGTTTTGGGATCCTTTGCTGGACAAGTTCAACGCTAAGCTGGACGCGTGGAGGAAGCTCCTCCTTTCTAAGGGTGGTAGAGTAACCTTGGCTCAATCCATCCTTAATAGCCTCCCAATTTATCACTTCTCCTTGCTAAAGGCTCCTAAGAAGGTGATAGATCGGATGGAGAAATTAATTAGAGATTTTGTTTGGAATGGTGGTACTTTCAAACCAGGTAGTAATCTTGTAAAATGGGAATGGACAGCTTTGCCAACATATTGTGGGGGCTTGGGAGTGGGGTCTCTAGCCAATTGGAACGTGGCCCTCCTTACTAAATGGTTATGGAGATTCACCCAAGAAGAAAGCGCCCTATGGAGAAAGGTGATTGCTAGCATTTACGGTGTCACGGCCATGGGATGGATGACTGTGCATTCGGCCAAGAAAATCGGGAGGAGATTCTGGCTCGACATCAACAAGTACACCTCCCAATTTACTAAATTTGCTAAATTCCAGGTTAATTGTGGCCAAAGAATCAGATTTTGGGATGATATTTGGTGTGATACTCGCCCTCTCAGTGTTGCTTTCCCAGACATCTATACTATCTCTCAAAAGAAAAATGCCTCCATTTATGAGTGTTGGGATGCAAACTCTCAAGCTTGGAATTTAGGCTTGCGAAGAGGCCTTTTTGACCGAGAAACCAGCAGTTGGGTGGCGCTAGTTGATAAGCTAAGTCTGGTGCAGCTGGGAGATGGTCCTGATAGAATTATTTGGAACTTGGATAATTCAGGGAGCTTTACCACTAATTCTCTGTTTAGAAATCAGATTGGGCATTCTTCTAAAGTCAACCACTCCCTGGCAGAGCTGATATGGAGGCACAAATGCCCCAAAAAAGTTAAAAAAAATTGTGGTCAGTAG

Coding sequence (CDS)

ATGACTGGAGAATTTTTCAAAAACTTTTGGAACATTTTGAAGCCCAACTTAGTAGAGGTGTTCCACGAGTTTTTTAAAAATGGTATCATCAATAAAAGAACAAACGAGACTTACATCTGCCTGATCCCTAAAAAGAGGAAGGCCTCAAATGTGAGAGATTTTCGACCTATTAGTCTGGTTACCTCCTTATACAAAATTATTGCAAAAGTCCTTGTTGAGAGGCTAAAGAAGGTCCTCCCTCAGACGATTAGTGATTGTCAAGTTGCTTTCGTTCACGGAAGGCAAATTTTAGATCCGATTCTTGTGGCCAACGAAACAGTGGAAGATTACAGGCTCGAAAAGAAAGAGGGTATATTGATGAAACTTGATCTGGAAAAAGCCTACGACAAAGTTAGTTGGAAGTTCCTCGATGCCATTCTAGCTTTAAAAGGTTTTGGTAAAACTTGGAGAATGTGGATCCAGAGGTGTCTTTTGAACACCAACTTCTCGGTGATCATCAATGGGAGACCTAGGGGGAAAATTCAAGCCTCTAGAGGACTTAGGCAAGGGGATCCCCTCTCCCCATTCCTTTTCACGATTGTGGGCGATGCCCTGAGCCGATCTGTTCAATATTGCATCGAGAAAAACATCCTAAAAGGCCTTGTTGTGGACAGAAACAGCATTGAGGTTACCATGCTCCAATACGCTGACGACACATTGATCTTCTGCCCAATTGTGAATGAGATTTTCGACAATTGGTGGGAGATTTTGAATATGATCCTAGAAGGGGCGGGGCTTAACTTGAATCTTGCAAAAACCTCCATCATAGGCATCGCCTCCATTATAGGCATCAATATCAATGAGGATACGATTAGGAATAAGGTGGTTAAAATGGGTTGTAGAGCTGAAAAGTTCCCGATAAATTACCTTGGCTTCCCCCTTGGGGGTAATCATCATACAGTTGCGTTTTGGGATCCTTTGCTGGACAAGTTCAACGCTAAGCTGGACGCGTGGAGGAAGCTCCTCCTTTCTAAGGGTGGTAGAGTAACCTTGGCTCAATCCATCCTTAATAGCCTCCCAATTTATCACTTCTCCTTGCTAAAGGCTCCTAAGAAGGTGATAGATCGGATGGAGAAATTAATTAGAGATTTTGTTTGGAATGGTGGTACTTTCAAACCAGGTAGTAATCTTGTAAAATGGGAATGGACAGCTTTGCCAACATATTGTGGGGGCTTGGGAGTGGGGTCTCTAGCCAATTGGAACGTGGCCCTCCTTACTAAATGGTTATGGAGATTCACCCAAGAAGAAAGCGCCCTATGGAGAAAGGTGATTGCTAGCATTTACGGTGTCACGGCCATGGGATGGATGACTGTGCATTCGGCCAAGAAAATCGGGAGGAGATTCTGGCTCGACATCAACAAGTACACCTCCCAATTTACTAAATTTGCTAAATTCCAGGTTAATTGTGGCCAAAGAATCAGATTTTGGGATGATATTTGGTGTGATACTCGCCCTCTCAGTGTTGCTTTCCCAGACATCTATACTATCTCTCAAAAGAAAAATGCCTCCATTTATGAGTGTTGGGATGCAAACTCTCAAGCTTGGAATTTAGGCTTGCGAAGAGGCCTTTTTGACCGAGAAACCAGCAGTTGGGTGGCGCTAGTTGATAAGCTAAGTCTGGTGCAGCTGGGAGATGGTCCTGATAGAATTATTTGGAACTTGGATAATTCAGGGAGCTTTACCACTAATTCTCTGTTTAGAAATCAGATTGGGCATTCTTCTAAAGTCAACCACTCCCTGGCAGAGCTGATATGGAGGCACAAATGCCCCAAAAAAGTTAAAAAAAATTGTGGTCAGTAG

Protein sequence

MTGEFFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLYKIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDLEKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGDPLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFDNWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLGFPLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPKKVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWRFTQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQRIRFWDDIWCDTRPLSVAFPDIYTISQKKNASIYECWDANSQAWNLGLRRGLFDRETSSWVALVDKLSLVQLGDGPDRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRHKCPKKVKKNCGQ
Homology
BLAST of Lag0041333.1 vs. NCBI nr
Match: XP_009335445.1 (PREDICTED: uncharacterized protein LOC103928158 [Pyrus x bretschneideri])

HSP 1 Score: 542.0 bits (1395), Expect = 6.8e-150
Identity = 277/608 (45.56%), Postives = 379/608 (62.34%), Query Frame = 0

Query: 4   EFFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSL 63
           + F++FW  LK ++V+V  EFF+ GIIN  TNET+ICLIPKK  +  V D+RPISLVT L
Sbjct: 282 QMFQHFWEFLKVDIVKVMEEFFETGIINAVTNETFICLIPKKSDSLKVTDYRPISLVTGL 341

Query: 64  YKIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLD 123
           YKI+AK LV RL++V+  TIS  Q  FV GRQILD +L+ANE VE+ R +K+EG++ K+D
Sbjct: 342 YKIVAKTLVSRLREVMGSTISPHQGGFVKGRQILDAVLIANELVEEVRQKKEEGLMFKID 401

Query: 124 LEKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQG 183
            EKAYD V W+FLD IL  KGFG  WR W+Q C  + NFS++INGRP+GK  ASR LRQG
Sbjct: 402 FEKAYDHVEWRFLDEILQRKGFGNRWRKWVQGCQNSANFSILINGRPKGKFHASRRLRQG 461

Query: 184 DPLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIF 243
           DPLSPFLFT+V D LSR ++   E +++KGL + +  +E++ LQ+ADDT+ F     +++
Sbjct: 462 DPLSPFLFTLVVDVLSRLMEKAHENHLIKGLGIGQERVEISHLQFADDTIFFLAEDEDVW 521

Query: 244 DNWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYL 303
           +N  E+LN+    +GL +N AK       S+ GIN + + ++      GC    +PI YL
Sbjct: 522 NNLLEVLNLFCTISGLKINKAK------CSMAGINSDCEKLKGMADSWGCEVGSWPIKYL 581

Query: 304 GFPLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAP 363
           G PLGG    + FWDP+++K   +L +W+K  LSKGGR+TL Q +L SLPIY+ SL K P
Sbjct: 582 GLPLGGRPKALMFWDPVVEKMEKRLQSWKKAFLSKGGRLTLIQFVLGSLPIYYMSLFKIP 641

Query: 364 KKVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLW 423
             VI R+EKL++ F+W G      ++LVKWE        GGL VG+L N   ALL KWLW
Sbjct: 642 CGVIGRLEKLMKGFLWEGVEEGKKNHLVKWEIVIKSKEEGGLCVGNLRNQKEALLAKWLW 701

Query: 424 RFTQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCG 483
           RF +E ++LW KVI S YG+   GW      +   R  W DI+     F +  KF+V  G
Sbjct: 702 RFPKEPNSLWHKVIRSKYGLQDNGWNANPPLRGSSRSPWKDISCGLQSFLQCCKFEVGNG 761

Query: 484 QRIRFWDDIWCDTRPLSVAFPDIYTISQKKNASIYECWD--ANSQAWNLGLRRGLFDRET 543
           +R+RFW+D W +  PL   +P ++ +S+  N +I    D   NS +WN   RR L   ET
Sbjct: 762 ERVRFWEDGWLEGGPLKEQYPRLFLLSRMHNQNISSFVDLSINSLSWNFDFRRNLNQVET 821

Query: 544 SSWVALVDKLSLVQLGDG-PDRIIWNLDNSGSFTTNSLFRNQIGHSSKVNH-SLAELIWR 603
                L+ K+  V+L     D   W L+ SG F   S +R+ + ++  V H   +  IW+
Sbjct: 822 EEAARLLQKVENVRLSQSRADDRRWKLELSGLFACKS-YRSSLSNNGIVQHFPPSSQIWK 881

Query: 604 HKCPKKVK 608
            K P KVK
Sbjct: 882 SKVPPKVK 882

BLAST of Lag0041333.1 vs. NCBI nr
Match: BBN69274.1 (TatD related DNase [Prunus dulcis])

HSP 1 Score: 535.0 bits (1377), Expect = 8.3e-148
Identity = 271/608 (44.57%), Postives = 386/608 (63.49%), Query Frame = 0

Query: 5   FFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLY 64
           FF++ W ++K +L++V  +FF++GI+N  TNET+ICLIPKK  +  V DFRPISLVTSLY
Sbjct: 218 FFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDFRPISLVTSLY 277

Query: 65  KIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDL 124
           K+I+KVL  RL++VL  TIS  Q AFV  RQILD +LVANE VE+ R + ++G++ K+D 
Sbjct: 278 KVISKVLASRLREVLGNTISQTQGAFVQKRQILDAVLVANEVVEEVRKQNRKGLVFKIDF 337

Query: 125 EKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGD 184
           EKAYD V WKF+D ++A KGFG  WR WI  CL + NFS++ING+PRGK +ASRGLRQGD
Sbjct: 338 EKAYDHVEWKFVDDVMARKGFGVKWRGWIFGCLESANFSIMINGKPRGKFRASRGLRQGD 397

Query: 185 PLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFD 244
           PLSPFLFT+V D LSR ++   + N++ G+V   + +EV+ LQ+ADDT+ F     E + 
Sbjct: 398 PLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHDQVEVSHLQFADDTIFFLDGKEEYWL 457

Query: 245 NWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLG 304
           N  ++L +  + +G+ +N AK      + I+GIN + +T+ N     GC    +P+ YLG
Sbjct: 458 NLLQMLKLFCDVSGMKINKAK------SCILGINFSIETLNNMAGSWGCEVGCWPMVYLG 517

Query: 305 FPLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPK 364
            PLGGN   + FW+P+LDK   +L  W++  LSKGGR+TL Q++L+S+P Y+ SL K P 
Sbjct: 518 LPLGGNPRALNFWNPVLDKVEKRLQRWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPI 577

Query: 365 KVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWR 424
            V  ++E+L+R+F+W G       +LV+W         GGLG+GSL   N AL  KWLWR
Sbjct: 578 GVTAKVEQLMRNFLWEGVEEGKNCHLVRWVRVTKSKEEGGLGIGSLRERNEALRAKWLWR 637

Query: 425 FTQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQ 484
           F  E ++LW ++I S YG+ + GW T    K   R  W +I+K    F +  +F V  G+
Sbjct: 638 FPLETNSLWHRIIKSKYGLDSNGWDTKQIDKVSCRNPWREISKGYISFLQCCRFSVGNGE 697

Query: 485 RIRFWDDIWCDTRPLSVAFPDIYTISQKKNASIYECWDANSQA----WNLGLRRGLFDRE 544
           +IRFW+D W     L   FP +Y++S++KN  I   W AN+      W+   RR L + E
Sbjct: 698 KIRFWEDFWLKEGILKDLFPRLYSLSRRKNQCI--AWFANNHEMPLNWDFDFRRNLSEAE 757

Query: 545 TSSWVALVDKLSLVQL-GDGPDRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWR 604
            +  V L+D L  V+L G  PDR  W ++  GSF+  S FR+ +  ++ V       IW+
Sbjct: 758 IAEVVLLLDILGNVRLFGSRPDRRSWEIEEQGSFSCKS-FRSFLISTTSVVFPPYRSIWK 816

Query: 605 HKCPKKVK 608
            K P K++
Sbjct: 818 AKTPPKIQ 816

BLAST of Lag0041333.1 vs. NCBI nr
Match: BBN69746.1 (VIRB2-interacting protein 2 [Prunus dulcis])

HSP 1 Score: 520.8 bits (1340), Expect = 1.6e-143
Identity = 272/605 (44.96%), Postives = 373/605 (61.65%), Query Frame = 0

Query: 6   FKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLYK 65
           F++ W+I+K +L++V  +FF  GIIN  TNET+ICLIPKK+++  V DFRPISLVTSLYK
Sbjct: 219 FQSCWDIVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESVKVSDFRPISLVTSLYK 278

Query: 66  IIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDLE 125
           +++KVL  RL++VL  TIS  Q AFV GRQILD  L+ANE VE+ R   K G++ K+DLE
Sbjct: 279 MVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGMVFKIDLE 338

Query: 126 KAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGDP 185
           KAYD V W+F+D +L  KGFG  WR WI+ CL   NFSV+INGRPRGK +ASRGLRQGDP
Sbjct: 339 KAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASRGLRQGDP 398

Query: 186 LSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFDN 245
           LSPFLFT+V D LSR ++   + +   GL      +E++ LQ+ADDT+ F     E ++N
Sbjct: 399 LSPFLFTLVMDVLSRIMEKAQDADEFHGLSPGNGMVEISHLQFADDTIFFIEDKEEYWNN 458

Query: 246 WWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLGF 305
             +IL +    +G+ +N +K       S++GIN+++  +       GC    +P+ YLG 
Sbjct: 459 LLQILELFCFVSGMTINKSK------CSLVGINLDDGMVNEMAGAWGCDVGVWPMLYLGL 518

Query: 306 PLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPKK 365
           PLGGN   + FWDP+++K   +L  W++  LSKGGR+T+ Q++L S+PIY+ SL + P  
Sbjct: 519 PLGGNPRAIKFWDPVVEKVENRLQKWKRACLSKGGRLTMIQAVLCSIPIYYMSLFRIPIG 578

Query: 366 VIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWRF 425
           V +R+EKL+RDF+W G   K  ++ V WE      +CGGLGVGSL   +VAL  KWLWRF
Sbjct: 579 VANRIEKLMRDFLWEGLDGK-SNHAVSWEVVGKAKFCGGLGVGSLRARSVALRAKWLWRF 638

Query: 426 TQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQR 485
             E  ALW KVI SIYG+   GW    + +   R  W DI+   + F +   F V  G R
Sbjct: 639 PNEPHALWHKVIRSIYGMDTNGWDAKPATRGSCRCPWRDISSGYNLFLQGCVFVVGSGVR 698

Query: 486 IRFWDDIWCDTRPLSVAFPDIYTISQKKNASI--YECWDANSQAWNLGLRRGLFDRETSS 545
           +RFW+D W     L   FP ++ +S+K+N +I  +   D    +W+ G RR L + E + 
Sbjct: 699 VRFWEDDWGRGGVLKEVFPRLFNLSRKQNHNISSFASSDGLLLSWDFGFRRNLNELEITE 758

Query: 546 WVALVDKLSLVQLGDGP-DRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRHKC 605
              L+D L  V+L     D+ IW  D SG FT +S   +               IW+ K 
Sbjct: 759 VARLLDLLEGVRLFTSRLDKRIWKFDPSGLFTCHSFCSHIQNCGEGEIFPPYTQIWKAKT 816

Query: 606 PKKVK 608
           P KVK
Sbjct: 819 PLKVK 816

BLAST of Lag0041333.1 vs. NCBI nr
Match: BBN67784.1 (VIRB2-interacting protein 2 [Prunus dulcis])

HSP 1 Score: 520.8 bits (1340), Expect = 1.6e-143
Identity = 272/605 (44.96%), Postives = 373/605 (61.65%), Query Frame = 0

Query: 6    FKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLYK 65
            F++ W+I+K +L++V  +FF  GIIN  TNET+ICLIPKK+++  V DFRPISLVTSLYK
Sbjct: 926  FQSCWDIVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESVKVSDFRPISLVTSLYK 985

Query: 66   IIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDLE 125
            +++KVL  RL++VL  TIS  Q AFV GRQILD  L+ANE VE+ R   K G++ K+DLE
Sbjct: 986  MVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGMVFKIDLE 1045

Query: 126  KAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGDP 185
            KAYD V W+F+D +L  KGFG  WR WI+ CL   NFSV+INGRPRGK +ASRGLRQGDP
Sbjct: 1046 KAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASRGLRQGDP 1105

Query: 186  LSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFDN 245
            LSPFLFT+V D LSR ++   + +   GL      +E++ LQ+ADDT+ F     E ++N
Sbjct: 1106 LSPFLFTLVMDVLSRIMEKAQDADEFHGLSPGNGMVEISHLQFADDTIFFIEDKEEYWNN 1165

Query: 246  WWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLGF 305
              +IL +    +G+ +N +K       S++GIN+++  +       GC    +P+ YLG 
Sbjct: 1166 LLQILELFCFVSGMTINKSK------CSLVGINLDDGMVNEMAGAWGCDVGVWPMLYLGL 1225

Query: 306  PLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPKK 365
            PLGGN   + FWDP+++K   +L  W++  LSKGGR+T+ Q++L S+PIY+ SL + P  
Sbjct: 1226 PLGGNPRAIKFWDPVVEKVENRLQKWKRACLSKGGRLTMIQAVLCSIPIYYMSLFRIPIG 1285

Query: 366  VIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWRF 425
            V +R+EKL+RDF+W G   K  ++ V WE      +CGGLGVGSL   +VAL  KWLWRF
Sbjct: 1286 VANRIEKLMRDFLWEGLDGK-SNHAVSWEVVGKAKFCGGLGVGSLRARSVALRAKWLWRF 1345

Query: 426  TQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQR 485
              E  ALW KVI SIYG+   GW    + +   R  W DI+   + F +   F V  G R
Sbjct: 1346 PNEPHALWHKVIRSIYGMDTNGWDAKPATRGSCRCPWRDISSGYNLFLQGCVFVVGSGVR 1405

Query: 486  IRFWDDIWCDTRPLSVAFPDIYTISQKKNASI--YECWDANSQAWNLGLRRGLFDRETSS 545
            +RFW+D W     L   FP ++ +S+K+N +I  +   D    +W+ G RR L + E + 
Sbjct: 1406 VRFWEDDWGRGGVLKEVFPRLFNLSRKQNHNISSFASSDGLLLSWDFGFRRNLNELEITE 1465

Query: 546  WVALVDKLSLVQLGDGP-DRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRHKC 605
               L+D L  V+L     D+ IW  D SG FT +S   +               IW+ K 
Sbjct: 1466 VARLLDLLEGVRLFTSRLDKRIWKFDPSGLFTCHSFCSHIQNCGEGEIFPPYTQIWKAKT 1523

Query: 606  PKKVK 608
            P KVK
Sbjct: 1526 PLKVK 1523

BLAST of Lag0041333.1 vs. NCBI nr
Match: BBG98819.1 (VIRB2-interacting protein 2 [Prunus dulcis] >BBH09158.1 VIRB2-interacting protein 2 [Prunus dulcis])

HSP 1 Score: 520.8 bits (1340), Expect = 1.6e-143
Identity = 272/605 (44.96%), Postives = 373/605 (61.65%), Query Frame = 0

Query: 6   FKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLYK 65
           F++ W+I+K +L++V  +FF  GIIN  TNET+ICLIPKK+++  V DFRPISLVTSLYK
Sbjct: 152 FQSCWDIVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESVKVSDFRPISLVTSLYK 211

Query: 66  IIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDLE 125
           +++KVL  RL++VL  TIS  Q AFV GRQILD  L+ANE VE+ R   K G++ K+DLE
Sbjct: 212 MVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGMVFKIDLE 271

Query: 126 KAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGDP 185
           KAYD V W+F+D +L  KGFG  WR WI+ CL   NFSV+INGRPRGK +ASRGLRQGDP
Sbjct: 272 KAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASRGLRQGDP 331

Query: 186 LSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFDN 245
           LSPFLFT+V D LSR ++   + +   GL      +E++ LQ+ADDT+ F     E ++N
Sbjct: 332 LSPFLFTLVMDVLSRIMEKAQDADEFHGLSPGNGMVEISHLQFADDTIFFIEDKEEYWNN 391

Query: 246 WWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLGF 305
             +IL +    +G+ +N +K       S++GIN+++  +       GC    +P+ YLG 
Sbjct: 392 LLQILELFCFVSGMTINKSK------CSLVGINLDDGMVNEMAGAWGCDVGVWPMLYLGL 451

Query: 306 PLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPKK 365
           PLGGN   + FWDP+++K   +L  W++  LSKGGR+T+ Q++L S+PIY+ SL + P  
Sbjct: 452 PLGGNPRAIKFWDPVVEKVENRLQKWKRACLSKGGRLTMIQAVLCSIPIYYMSLFRIPIG 511

Query: 366 VIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWRF 425
           V +R+EKL+RDF+W G   K  ++ V WE      +CGGLGVGSL   +VAL  KWLWRF
Sbjct: 512 VANRIEKLMRDFLWEGLDGK-SNHAVSWEVVGKAKFCGGLGVGSLRARSVALRAKWLWRF 571

Query: 426 TQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQR 485
             E  ALW KVI SIYG+   GW    + +   R  W DI+   + F +   F V  G R
Sbjct: 572 PNEPHALWHKVIRSIYGMDTNGWDAKPATRGSCRCPWRDISSGYNLFLQGCVFVVGSGVR 631

Query: 486 IRFWDDIWCDTRPLSVAFPDIYTISQKKNASI--YECWDANSQAWNLGLRRGLFDRETSS 545
           +RFW+D W     L   FP ++ +S+K+N +I  +   D    +W+ G RR L + E + 
Sbjct: 632 VRFWEDDWGRGGVLKEVFPRLFNLSRKQNHNISSFASSDGLLLSWDFGFRRNLNELEITE 691

Query: 546 WVALVDKLSLVQLGDGP-DRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRHKC 605
              L+D L  V+L     D+ IW  D SG FT +S   +               IW+ K 
Sbjct: 692 VARLLDLLEGVRLFTSRLDKRIWKFDPSGLFTCHSFCSHIQNCGEGEIFPPYTQIWKAKT 749

Query: 606 PKKVK 608
           P KVK
Sbjct: 752 PLKVK 749

BLAST of Lag0041333.1 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.9e-31
Identity = 109/434 (25.12%), Postives = 202/434 (46.54%), Query Frame = 0

Query: 2   TGEFFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKK-RKASNVRDFRPISLV 61
           T EF++ +   L P L+++F    K GI+     E  I LIPK  R  +   +FRPISL+
Sbjct: 477 TAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLM 536

Query: 62  TSLYKIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDY-RLEKKEGIL 121
               KI+ K+L  R+++ + + I   QV F+ G Q    I  +   ++   R + K  ++
Sbjct: 537 NIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVI 596

Query: 122 MKLDLEKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRG 181
           + +D EKA+DK+   F+   L   G    +   I+        ++I+NG+         G
Sbjct: 597 ISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTG 656

Query: 182 LRQGDPLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFC--P 241
            RQG PLSP LF IV + L+R+++   ++  +KG+ + +  +++++  +ADD +++   P
Sbjct: 657 TRQGCPLSPLLFNIVLEVLARAIR---QEKEIKGIQLGKEEVKLSL--FADDMIVYLENP 716

Query: 242 IVNEIFDNWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEK 301
           IV+    N  ++++   + +G  +N+ K      +     N N  T    + ++      
Sbjct: 717 IVSA--QNLLKLISNFSKVSGYKINVQK------SQAFLYNNNRQTESQIMGELPFTIAS 776

Query: 302 FPINYLGFPLGGNHHTV--AFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIY 361
             I YLG  L  +   +    + PLL +     + W+ +  S  GR+ + +  +    IY
Sbjct: 777 KRIKYLGIQLTRDVKDLFKENYKPLLKEIKEDTNKWKNIPCSWVGRINIVKMAILPKVIY 836

Query: 362 HFSL--LKAPKKVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANW 421
            F+   +K P      +EK    F+WN    +   +++  +  A     GG+ +     +
Sbjct: 837 RFNAIPIKLPMTFFTELEKTTLKFIWNQKRARIAKSILSQKNKA-----GGITLPDFKLY 892

Query: 422 NVALLTKWLWRFTQ 428
             A +TK  W + Q
Sbjct: 897 YKATVTKTAWYWYQ 892

BLAST of Lag0041333.1 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.5e-30
Identity = 112/445 (25.17%), Postives = 205/445 (46.07%), Query Frame = 0

Query: 2   TGEFFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRK-ASNVRDFRPISLV 61
           T EF++ F   L P L+ +F    K GI+     E  I LIPK  K  +   ++RPISL+
Sbjct: 476 TSEFYQTFKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLM 535

Query: 62  TSLYKIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDY-RLEKKEGIL 121
               KI+ K+L  R+++ + + I   QV F+ G Q    I  +   ++   +L+ K+ ++
Sbjct: 536 NIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDHMI 595

Query: 122 MKLDLEKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRG 181
           + +D EKA+D +   F+   L   G   T+   I+        ++I+NG          G
Sbjct: 596 LSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSG 655

Query: 182 LRQGDPLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIV 241
            RQG PLSP LF IV + L+ +++   E+  +KG+ +    I++++  +ADD +++    
Sbjct: 656 TRQGCPLSPLLFNIVMEVLAIAIR---EEKAIKGIHIGSEEIKLSL--FADDMIVYLENT 715

Query: 242 NEIFDNWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFP 301
            +      E++      +G  +N  K+      + I  N N+     K VK        P
Sbjct: 716 RDSTTKLLEVIKEYSNVSGYKINTHKS-----VAFIYTNNNQ---AEKTVKDSIPFTVVP 775

Query: 302 --INYLGFPLGGNHHTV--AFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIY 361
             + YLG  L  +   +    ++ L  +    ++ W+ +  S  GR+ + +  +    IY
Sbjct: 776 KKMKYLGVYLTKDVKDLYKENYETLRKEIAEDVNKWKNIPCSWLGRINIVKMSILPKAIY 835

Query: 362 HFSL--LKAPKKVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANW 421
           +F+   +KAP      +EK+I  F+WN    +    L+  +  A     GG+ +  L  +
Sbjct: 836 NFNAIPIKAPLSYFKDLEKIILHFIWNQKKPQIAKTLLSNKNKA-----GGITLPDLRLY 895

Query: 422 --NVALLTKWLWRFTQEESALWRKV 437
             ++ + T W W   +E   +W ++
Sbjct: 896 YKSIVIKTAWYWHKNREVD-VWNRI 901

BLAST of Lag0041333.1 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 1.2e-29
Identity = 114/455 (25.05%), Postives = 208/455 (45.71%), Query Frame = 0

Query: 2   TGEFFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRK-ASNVRDFRPISLV 61
           + EF++ F   L P L ++FH+    G +     E  I LIPK +K  + + +FRPISL+
Sbjct: 484 SAEFYQTFKEDLIPILHKLFHKIEVEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLM 543

Query: 62  TSLYKIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDY-RLEKKEGIL 121
               KI+ K+L  R+++ +   I   QV F+ G Q    I  +   +    +L+ K  ++
Sbjct: 544 NIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGWFNIRKSINVIHYINKLKDKNHMI 603

Query: 122 MKLDLEKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRG 181
           + LD EKA+DK+   F+  +L   G    +   I+        ++ +NG     I    G
Sbjct: 604 ISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSG 663

Query: 182 LRQGDPLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIV 241
            RQG PLSP+LF IV + L+R+++   ++  +KG+ + +  +++++L  ADD +++   +
Sbjct: 664 TRQGCPLSPYLFNIVLEVLARAIR---QQKEIKGIQIGKEEVKISLL--ADDMIVY---I 723

Query: 242 NEIFDNWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEK-- 301
           ++  ++  E+LN+               I     ++G  IN +     +     +AEK  
Sbjct: 724 SDPKNSTRELLNL---------------INSFGEVVGYKINSNKSMAFLYTKNKQAEKEI 783

Query: 302 ----------FPINYLGFPLGGNHHTV--AFWDPLLDKFNAKLDAWRKLLLSKGGRVTLA 361
                       I YLG  L      +    +  L  +    L  W+ L  S  GR+ + 
Sbjct: 784 RETTPFSIVTNNIKYLGVTLTKEVKDLYDKNFKSLKKEIKEDLRRWKDLPCSWIGRINIV 843

Query: 362 QSILNSLPIYHFSL--LKAPKKVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCG 421
           +  +    IY F+   +K P +  + +E  I  FVWN    +   +L+K + T+     G
Sbjct: 844 KMAILPKAIYRFNAIPIKIPTQFFNELEGAICKFVWNNKKPRIAKSLLKDKRTS-----G 903

Query: 422 GLGVGSLANWNVALL--TKWLWRFTQEESALWRKV 437
           G+ +  L  +  A++  T W W +   +   W ++
Sbjct: 904 GITMPDLKLYYRAIVIKTAWYW-YRDRQVDQWNRI 909

BLAST of Lag0041333.1 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 4.7e-29
Identity = 111/445 (24.94%), Postives = 200/445 (44.94%), Query Frame = 0

Query: 1   MTGEFFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLV 60
           +T EFF+ FW+ L P+   V  E FK G +        + L+PKK     ++++RP+SL+
Sbjct: 472 LTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLL 531

Query: 61  TSLYKIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILM 120
           ++ YKI+AK +  RLK VL + I   Q   V GR I D + +  + +   R        +
Sbjct: 532 STDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHFARRTGLSLAFL 591

Query: 121 KLDLEKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGL 180
            LD EKA+D+V  ++L   L    FG  +  +++    +    V IN      +   RG+
Sbjct: 592 SLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRGV 651

Query: 181 RQGDPLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVN 240
           RQG PLS  L+++  +        C+ +  L GLV+    + V +  YADD ++    + 
Sbjct: 652 RQGCPLSGQLYSLAIEPF-----LCLLRKRLTGLVLKEPDMRVVLSAYADDVILVAQDLV 711

Query: 241 EIFDNWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPI 300
           ++ +   E   +    +   +N +K+S + +   + ++      R+         E   I
Sbjct: 712 DL-ERAQECQEVYAAASSARINWSKSSGL-LEGSLKVDFLPPAFRD------ISWESKII 771

Query: 301 NYLGFPLGGNHHTVA-FWDPLLDKFNAKLDAWRKL--LLSKGGRVTLAQSILNSLPIYHF 360
            YLG  L    + V+  +  L +    +L  W+    +LS  GR  +   ++ S   Y  
Sbjct: 772 KYLGVYLSAEEYPVSQNFIELEECVLTRLGKWKGFAKVLSMRGRALVINQLVASQIWYRL 831

Query: 361 SLLKAPKKVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVAL 420
             L   ++ I ++++ + DF+W       G + V    ++LP   GG GV  + +     
Sbjct: 832 ICLSPTQEFIAKIQRRLLDFLW------IGKHWVSAGVSSLPLKEGGQGVVCIRSQVHTF 891

Query: 421 LTKWLWRFT-QEESALWRKVIASIY 442
             + + R+   + S  W  + +S Y
Sbjct: 892 RLQQIQRYLYADPSPQWCTLASSFY 897

BLAST of Lag0041333.1 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 3.5e-24
Identity = 80/306 (26.14%), Postives = 141/306 (46.08%), Query Frame = 0

Query: 320 LLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPKKVIDRMEKLIRDFVW 379
           +L++ ++++  WR+  LS  GR+TL +++L+S+P++  S +  P+ +++R+++L R F+W
Sbjct: 16  ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 380 NGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWRFTQEESALWRKVIAS 439
                K   +LVKW     P   GGLGV +  + N AL++K  WR  QE+++LW  V+  
Sbjct: 76  GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 440 IYGVTAM----------GWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQRIRFW 499
            Y V  +           W +   +  IG R   D+  +   +          GQ+IRFW
Sbjct: 136 KYHVGEIRDSRWLIPKGSWSSTWRSIAIGLR---DVVSHGVGWIP------GDGQQIRFW 195

Query: 500 DDIWCDTRPL-----SVAFPDIYTISQKKNASIYECWDANSQAWNLGLRRGLFDRETSSW 559
            D W   +PL          D  T+  K      + W    + W+        D  T++ 
Sbjct: 196 TDRWVSGKPLLELDNGERPTDCDTVVAK------DLW-IPGRGWDF----AKIDPYTTNN 255

Query: 560 VALVDKLSLVQLGDGP-DRIIWNLDNSGSFTTNSLFRN-QIGHSSKVN-HSLAELIWRHK 608
             L  +  ++ L  G  DR+ W     G F+  S +    +    + N  S    +W+ +
Sbjct: 256 TRLELRAVVLDLVTGARDRLSWKFSQDGQFSVRSAYEMLTVDEVPRPNMASFFNCLWKVR 301

BLAST of Lag0041333.1 vs. ExPASy TrEMBL
Match: A0A5H2XQW2 (TatD related DNase OS=Prunus dulcis OX=3755 GN=Prudu_862S001100 PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 4.0e-148
Identity = 271/608 (44.57%), Postives = 386/608 (63.49%), Query Frame = 0

Query: 5   FFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLY 64
           FF++ W ++K +L++V  +FF++GI+N  TNET+ICLIPKK  +  V DFRPISLVTSLY
Sbjct: 218 FFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDFRPISLVTSLY 277

Query: 65  KIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDL 124
           K+I+KVL  RL++VL  TIS  Q AFV  RQILD +LVANE VE+ R + ++G++ K+D 
Sbjct: 278 KVISKVLASRLREVLGNTISQTQGAFVQKRQILDAVLVANEVVEEVRKQNRKGLVFKIDF 337

Query: 125 EKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGD 184
           EKAYD V WKF+D ++A KGFG  WR WI  CL + NFS++ING+PRGK +ASRGLRQGD
Sbjct: 338 EKAYDHVEWKFVDDVMARKGFGVKWRGWIFGCLESANFSIMINGKPRGKFRASRGLRQGD 397

Query: 185 PLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFD 244
           PLSPFLFT+V D LSR ++   + N++ G+V   + +EV+ LQ+ADDT+ F     E + 
Sbjct: 398 PLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHDQVEVSHLQFADDTIFFLDGKEEYWL 457

Query: 245 NWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLG 304
           N  ++L +  + +G+ +N AK      + I+GIN + +T+ N     GC    +P+ YLG
Sbjct: 458 NLLQMLKLFCDVSGMKINKAK------SCILGINFSIETLNNMAGSWGCEVGCWPMVYLG 517

Query: 305 FPLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPK 364
            PLGGN   + FW+P+LDK   +L  W++  LSKGGR+TL Q++L+S+P Y+ SL K P 
Sbjct: 518 LPLGGNPRALNFWNPVLDKVEKRLQRWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPI 577

Query: 365 KVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWR 424
            V  ++E+L+R+F+W G       +LV+W         GGLG+GSL   N AL  KWLWR
Sbjct: 578 GVTAKVEQLMRNFLWEGVEEGKNCHLVRWVRVTKSKEEGGLGIGSLRERNEALRAKWLWR 637

Query: 425 FTQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQ 484
           F  E ++LW ++I S YG+ + GW T    K   R  W +I+K    F +  +F V  G+
Sbjct: 638 FPLETNSLWHRIIKSKYGLDSNGWDTKQIDKVSCRNPWREISKGYISFLQCCRFSVGNGE 697

Query: 485 RIRFWDDIWCDTRPLSVAFPDIYTISQKKNASIYECWDANSQA----WNLGLRRGLFDRE 544
           +IRFW+D W     L   FP +Y++S++KN  I   W AN+      W+   RR L + E
Sbjct: 698 KIRFWEDFWLKEGILKDLFPRLYSLSRRKNQCI--AWFANNHEMPLNWDFDFRRNLSEAE 757

Query: 545 TSSWVALVDKLSLVQL-GDGPDRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWR 604
            +  V L+D L  V+L G  PDR  W ++  GSF+  S FR+ +  ++ V       IW+
Sbjct: 758 IAEVVLLLDILGNVRLFGSRPDRRSWEIEEQGSFSCKS-FRSFLISTTSVVFPPYRSIWK 816

Query: 605 HKCPKKVK 608
            K P K++
Sbjct: 818 AKTPPKIQ 816

BLAST of Lag0041333.1 vs. ExPASy TrEMBL
Match: M5WKV4 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa015607mg PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.7e-146
Identity = 269/607 (44.32%), Postives = 384/607 (63.26%), Query Frame = 0

Query: 5   FFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLY 64
           FF++ W ++K +L++V  +FF++GI+N  TNET+ICLIPKK  +  V D+RPISLVTSLY
Sbjct: 205 FFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDYRPISLVTSLY 264

Query: 65  KIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDL 124
           K+I+KVL  RL++VL  TIS  Q AFV  RQILD +LVANE VE+ R +K++G++ K+D 
Sbjct: 265 KVISKVLASRLREVLGNTISQSQGAFVQKRQILDAVLVANEVVEEVRKQKRKGLVFKIDF 324

Query: 125 EKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGD 184
           EKAYD V W F+D +LA KGFG  WR WI  CL + NFS++ING+PRGK +ASRGLRQGD
Sbjct: 325 EKAYDHVEWNFVDDVLARKGFGAKWRGWIIGCLESVNFSIMINGKPRGKFRASRGLRQGD 384

Query: 185 PLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFD 244
           PLSPFLFT+V D LSR ++   + N++ G+V   + +EV+ LQ+ADDT+       E + 
Sbjct: 385 PLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHDQVEVSHLQFADDTIFLLDGKEEYWL 444

Query: 245 NWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLG 304
           N  ++L +  E +G+ +N AK      + I+GIN + D + N     GC    +P+ YLG
Sbjct: 445 NLLQLLKLFCEVSGMKINKAK------SCILGINFSTDALNNMAGSWGCEVGCWPMVYLG 504

Query: 305 FPLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPK 364
            PLGGN   + FW+P++DK   +L  W++  LSKGGR+TL Q++L+S+P Y+ SL K P 
Sbjct: 505 LPLGGNPRALNFWNPVMDKVEKRLQKWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPI 564

Query: 365 KVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWR 424
            V  ++E+L+R+F+W G       +LV+WE        GGLG+GSL   N AL  KWLWR
Sbjct: 565 GVAAKVEQLMRNFLWEGLEDGKNCHLVRWERVTKSKEEGGLGIGSLRERNEALRAKWLWR 624

Query: 425 FTQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQ 484
           F  E ++LW ++I S YG+ + GW T    K   R  W +I+K  + F +  +F V  G+
Sbjct: 625 FPLEPNSLWHRIIKSKYGIDSNGWDTKQIDKVSCRNPWREISKGYNSFLQCCRFSVGNGE 684

Query: 485 RIRFWDDIWCDTRPLSVAFPDIYTISQKKNASIYECWDANSQA---WNLGLRRGLFDRET 544
           +IRFW+D W     L   FP + ++S++KN SI  C+  N      W+   RR L + E 
Sbjct: 685 KIRFWEDFWLKEGILKDLFPRLSSLSRRKNQSI-ACFANNHVMPLNWDFDFRRNLSEAEI 744

Query: 545 SSWVALVDKLSLVQL-GDGPDRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRH 604
           +  V L+D L  V+L G  PDR  W +   GSF+  S FR+ +  +++        IW+ 
Sbjct: 745 AEVVILLDILGNVRLYGSRPDRRSWEVKEQGSFSCKS-FRSFLLSTTRDVFPPFSSIWKA 803

Query: 605 KCPKKVK 608
           K P K++
Sbjct: 805 KTPPKIQ 803

BLAST of Lag0041333.1 vs. ExPASy TrEMBL
Match: M5VS59 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa016504mg PE=4 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.9e-145
Identity = 267/607 (43.99%), Postives = 385/607 (63.43%), Query Frame = 0

Query: 5    FFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLY 64
            FF++ W ++K +L++V  +FF++GI+N  TNET+ICLIPKK  +  V D RPISLVTSLY
Sbjct: 449  FFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDNRPISLVTSLY 508

Query: 65   KIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDL 124
            K+I+KVL  RL++VL  TIS  Q AFV  RQILD +LVANE VE+ R +K++G++ K+D 
Sbjct: 509  KVISKVLASRLREVLGNTISQSQGAFVQKRQILDAVLVANEVVEEVRKQKRKGLVFKIDF 568

Query: 125  EKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGD 184
            EKAYD V W F+D +LA KGFG  WR WI  CL + NFS++ING+PRGK +ASRGLRQGD
Sbjct: 569  EKAYDHVEWNFVDDVLARKGFGVKWRGWIIGCLESVNFSIMINGKPRGKFRASRGLRQGD 628

Query: 185  PLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFD 244
            PLSPFLFT+V D LSR ++   + N++ G+V   + +EV+ LQ+ADDT+       E + 
Sbjct: 629  PLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHDQVEVSHLQFADDTIFLLDGKEEYWL 688

Query: 245  NWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLG 304
            N  ++L +  + +G+ +N AK      + I+GIN + + + N     GC    +P+ YLG
Sbjct: 689  NLLQLLKLFCDVSGMKINKAK------SCILGINFSTEALNNMAGSWGCEVGCWPMVYLG 748

Query: 305  FPLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPK 364
             PLGGN   + FW+P++DK   +L  W++  LSKGGR+TL Q++L+S+P Y+ SL K P 
Sbjct: 749  LPLGGNPRALNFWNPVMDKVEKRLQKWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPI 808

Query: 365  KVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWR 424
             V  ++E+L+R+F+W G       +LV+WE        GGLG+GSL   N AL  KWLWR
Sbjct: 809  GVAAKVEQLMRNFLWEGLEEGKNCHLVRWERVTKSKEEGGLGIGSLRERNEALRAKWLWR 868

Query: 425  FTQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQ 484
            F  E ++LW ++I S YG+ + GW T    K   R  W +I+K  + F +  +F V  G+
Sbjct: 869  FPLEPNSLWHRIIKSKYGIDSNGWDTKQIDKVSCRNPWREISKGYNSFLQCCRFSVGNGE 928

Query: 485  RIRFWDDIWCDTRPLSVAFPDIYTISQKKNASIYECWDANSQA---WNLGLRRGLFDRET 544
            +IRFW+D+W     L   FP + ++S++KN SI  C+  N      W+   RR L + E 
Sbjct: 929  KIRFWEDLWLKEGILKDLFPRLSSLSRRKNQSI-ACFANNHVMPLNWDFDFRRNLSEAEI 988

Query: 545  SSWVALVDKLSLVQL-GDGPDRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRH 604
            +  V L+D L  V+L G  PDR  W ++  GSF+  S FR+ +  +++        IW+ 
Sbjct: 989  AEVVILLDILGNVRLYGSRPDRRSWEVEEQGSFSCKS-FRSFLLSTTRDVFPPFSSIWKA 1047

Query: 605  KCPKKVK 608
            K P K++
Sbjct: 1049 KTPPKIQ 1047

BLAST of Lag0041333.1 vs. ExPASy TrEMBL
Match: M5X4S0 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa021750mg PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 3.2e-145
Identity = 265/607 (43.66%), Postives = 386/607 (63.59%), Query Frame = 0

Query: 5   FFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLY 64
           FF++ W ++K +L++V  +FF++GI+N  TNET+ICLIPKK  +  V D+RPISLVTSLY
Sbjct: 198 FFQSCWEVVKGDLMKVMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDYRPISLVTSLY 257

Query: 65  KIIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDL 124
           K+I+KVL  RL++VL  TIS  Q AFV  RQILD +LVANE VE+ R +K++G++ K+D 
Sbjct: 258 KVISKVLASRLREVLGNTISQSQGAFVQKRQILDAVLVANEVVEEVRKQKRKGLVFKIDF 317

Query: 125 EKAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGD 184
           EKAYD V W F+D ++A KGFG  WR WI  CL + NFS++ING+PRGK +ASRGLRQGD
Sbjct: 318 EKAYDHVEWNFVDDVMARKGFGVKWRGWIIGCLESVNFSIMINGKPRGKFRASRGLRQGD 377

Query: 185 PLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFD 244
           PLSPFLFT+V D LSR ++   + N++ G+V   + +EV+ LQ+ADDT+       E + 
Sbjct: 378 PLSPFLFTLVSDVLSRLIERAQDVNLVHGIVSGHDQVEVSHLQFADDTIFLLDGKEEYWL 437

Query: 245 NWWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLG 304
           N  ++L +  + +G+ +N AK      + I+GIN + + + N     GC    +P+ YLG
Sbjct: 438 NLLQLLKLFCDVSGMKINKAK------SCILGINFSTEVLNNMAGSWGCEVGCWPMVYLG 497

Query: 305 FPLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPK 364
            PLGGN   + FW+P+++K   +L  W++  LSKGGR+TL Q++L+S+P Y+ SL K P 
Sbjct: 498 LPLGGNPRALNFWNPVMEKVEKRLQKWKRACLSKGGRLTLIQAVLSSIPSYYMSLFKMPI 557

Query: 365 KVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWR 424
            V  ++E+L+R+F+W G       +LV+WE        GGLG+GSL   N AL  KWLWR
Sbjct: 558 GVAAKVEQLMRNFLWEGLEEGKKCHLVRWERVTKSKEEGGLGIGSLRERNEALRAKWLWR 617

Query: 425 FTQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQ 484
           F  E ++LW ++I S YG+ + GW T    K   R  W +I+K  + F +  +F V  G+
Sbjct: 618 FPLETNSLWHRIIKSKYGIDSNGWDTKRIDKVSCRNPWREISKGYNSFLQCCRFSVGNGE 677

Query: 485 RIRFWDDIWCDTRPLSVAFPDIYTISQKKNASIYECWDANSQA---WNLGLRRGLFDRET 544
           +IRFW+D+W     L   FP + ++S++KN SI  C+  N      W+   RR L + E 
Sbjct: 678 KIRFWEDLWLKEGILKDLFPRLSSLSRRKNQSI-ACFANNHVLPLNWDFDFRRNLSEAEI 737

Query: 545 SSWVALVDKLSLVQL-GDGPDRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRH 604
           +  V L+D L  V+L G  PDR  W ++  GSF+  S FR+ +  +++        IW+ 
Sbjct: 738 AEVVILLDILGNVRLYGSRPDRRSWEVEEQGSFSCKS-FRSFLLSTTRDVFPPFSSIWKA 796

Query: 605 KCPKKVK 608
           K P K++
Sbjct: 798 KTPPKIQ 796

BLAST of Lag0041333.1 vs. ExPASy TrEMBL
Match: A0A4Y1R3V4 (VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_008314 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 7.8e-144
Identity = 272/605 (44.96%), Postives = 373/605 (61.65%), Query Frame = 0

Query: 6   FKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVTSLYK 65
           F++ W+I+K +L++V  +FF  GIIN  TNET+ICLIPKK+++  V DFRPISLVTSLYK
Sbjct: 152 FQSCWDIVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESVKVSDFRPISLVTSLYK 211

Query: 66  IIAKVLVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEKKEGILMKLDLE 125
           +++KVL  RL++VL  TIS  Q AFV GRQILD  L+ANE VE+ R   K G++ K+DLE
Sbjct: 212 MVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGMVFKIDLE 271

Query: 126 KAYDKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGRPRGKIQASRGLRQGDP 185
           KAYD V W+F+D +L  KGFG  WR WI+ CL   NFSV+INGRPRGK +ASRGLRQGDP
Sbjct: 272 KAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASRGLRQGDP 331

Query: 186 LSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVTMLQYADDTLIFCPIVNEIFDN 245
           LSPFLFT+V D LSR ++   + +   GL      +E++ LQ+ADDT+ F     E ++N
Sbjct: 332 LSPFLFTLVMDVLSRIMEKAQDADEFHGLSPGNGMVEISHLQFADDTIFFIEDKEEYWNN 391

Query: 246 WWEILNMILEGAGLNLNLAKTSIIGIASIIGININEDTIRNKVVKMGCRAEKFPINYLGF 305
             +IL +    +G+ +N +K       S++GIN+++  +       GC    +P+ YLG 
Sbjct: 392 LLQILELFCFVSGMTINKSK------CSLVGINLDDGMVNEMAGAWGCDVGVWPMLYLGL 451

Query: 306 PLGGNHHTVAFWDPLLDKFNAKLDAWRKLLLSKGGRVTLAQSILNSLPIYHFSLLKAPKK 365
           PLGGN   + FWDP+++K   +L  W++  LSKGGR+T+ Q++L S+PIY+ SL + P  
Sbjct: 452 PLGGNPRAIKFWDPVVEKVENRLQKWKRACLSKGGRLTMIQAVLCSIPIYYMSLFRIPIG 511

Query: 366 VIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSLANWNVALLTKWLWRF 425
           V +R+EKL+RDF+W G   K  ++ V WE      +CGGLGVGSL   +VAL  KWLWRF
Sbjct: 512 VANRIEKLMRDFLWEGLDGK-SNHAVSWEVVGKAKFCGGLGVGSLRARSVALRAKWLWRF 571

Query: 426 TQEESALWRKVIASIYGVTAMGWMTVHSAKKIGRRFWLDINKYTSQFTKFAKFQVNCGQR 485
             E  ALW KVI SIYG+   GW    + +   R  W DI+   + F +   F V  G R
Sbjct: 572 PNEPHALWHKVIRSIYGMDTNGWDAKPATRGSCRCPWRDISSGYNLFLQGCVFVVGSGVR 631

Query: 486 IRFWDDIWCDTRPLSVAFPDIYTISQKKNASI--YECWDANSQAWNLGLRRGLFDRETSS 545
           +RFW+D W     L   FP ++ +S+K+N +I  +   D    +W+ G RR L + E + 
Sbjct: 632 VRFWEDDWGRGGVLKEVFPRLFNLSRKQNHNISSFASSDGLLLSWDFGFRRNLNELEITE 691

Query: 546 WVALVDKLSLVQLGDGP-DRIIWNLDNSGSFTTNSLFRNQIGHSSKVNHSLAELIWRHKC 605
              L+D L  V+L     D+ IW  D SG FT +S   +               IW+ K 
Sbjct: 692 VARLLDLLEGVRLFTSRLDKRIWKFDPSGLFTCHSFCSHIQNCGEGEIFPPYTQIWKAKT 749

Query: 606 PKKVK 608
           P KVK
Sbjct: 752 PLKVK 749

BLAST of Lag0041333.1 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 2.0e-11
Identity = 56/248 (22.58%), Postives = 96/248 (38.71%), Query Frame = 0

Query: 351 SLPIYHFSLLKAPKKVIDRMEKLIRDFVWNGGTFKPGSNLVKWEWTALPTYCGGLGVGSL 410
           +LP Y  +    PK V  ++  ++ DF W       G +   W+  +     GG+G   +
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 411 ANWNVALLTKWLWRFTQEESALWRKVIASIY------------GVTAMGWMTVHSAKKIG 470
             +N+ALL K +WR      +L  KV  S Y               +  W ++H++++I 
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 471 RRFWLDINKYTSQFTKFAKFQVNCGQRIRFWDDIWCDTRPLSVAF-----PDIYTISQKK 530
           R              + A+  V  G+ I  W   W D++P S A      P     S   
Sbjct: 122 R--------------QGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSS 181

Query: 531 NASIYECWDANSQAWNLGLRRGLFDRETSSWVALVDKLSLVQLGDGPDRII----WNLDN 578
              + +  D + + W   +   LF          V++  + +L  G  RI+    W+  +
Sbjct: 182 ILKVSDLIDESGREWRKDVIEMLFPE--------VERKLIGELRPGGRRILDSYTWDYTS 227

BLAST of Lag0041333.1 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 67.8 bits (164), Expect = 3.5e-11
Identity = 33/68 (48.53%), Postives = 43/68 (63.24%), Query Frame = 0

Query: 165 IINGRPRGKIQASRGLRQGDPLSPFLFTIVGDALSRSVQYCIEKNILKGLVVDRNSIEVT 224
           IING P+G +  SRGLRQGDPLSP+LF +  + LS   +   E+  L G+ V  NS  + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 225 MLQYADDT 233
            L +ADDT
Sbjct: 73  HLLFADDT 80

BLAST of Lag0041333.1 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 67.4 bits (163), Expect = 4.6e-11
Identity = 44/126 (34.92%), Postives = 61/126 (48.41%), Query Frame = 0

Query: 71  LVERLKKVLPQTISDCQVAFVHGRQILDPILVANETVEDYRLEK--KEGILMKLDLEKAY 130
           +VERLK ++   I   Q +F+ GR   D I+   E V   R +K  K  +L+KLDLEKAY
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 131 DKVSWKFLDAILALKGFGKTWRMWIQRCLLNTNFSVIINGR----PRGKIQASR-GLRQG 190
           D++ W +L+  L   GF + W   I R            GR     R ++   R G R  
Sbjct: 61  DRIRWDYLEDTLISAGFPEVWLPEIARSTFGARRVAPEVGRADASKRPRVSDHRWGFRYD 120

BLAST of Lag0041333.1 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 4.0e-07
Identity = 28/66 (42.42%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 2   TGEFFKNFWNILKPNLVEVFHEFFKNGIINKRTNETYICLIPKKRKASNVRDFRPISLVT 61
           T EFF   W ++K + +    EFF+ G + KR N T I LIPK      +  FRP+S  T
Sbjct: 555 TAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCT 614

Query: 62  SLYKII 68
            +YKII
Sbjct: 615 VVYKII 620

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_009335445.16.8e-15045.56PREDICTED: uncharacterized protein LOC103928158 [Pyrus x bretschneideri][more]
BBN69274.18.3e-14844.57TatD related DNase [Prunus dulcis][more]
BBN69746.11.6e-14344.96VIRB2-interacting protein 2 [Prunus dulcis][more]
BBN67784.11.6e-14344.96VIRB2-interacting protein 2 [Prunus dulcis][more]
BBG98819.11.6e-14344.96VIRB2-interacting protein 2 [Prunus dulcis] >BBH09158.1 VIRB2-interacting protei... [more]
Match NameE-valueIdentityDescription
O003703.9e-3125.12LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P085481.5e-3025.17LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
P113691.2e-2925.05LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P143814.7e-2924.94Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
P0C2F63.5e-2426.14Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A5H2XQW24.0e-14844.57TatD related DNase OS=Prunus dulcis OX=3755 GN=Prudu_862S001100 PE=4 SV=1[more]
M5WKV41.7e-14644.32Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
M5VS591.9e-14543.99Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
M5X4S03.2e-14543.66Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
A0A4Y1R3V47.8e-14444.96VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_008314 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.12.0e-1122.58Ribonuclease H-like superfamily protein [more]
ATMG01250.13.5e-1148.53RNA-directed DNA polymerase (reverse transcriptase) [more]
AT4G20520.14.6e-1134.92RNA binding;RNA-directed DNA polymerases [more]
AT1G43760.14.0e-0742.42DNAse I-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 42..307
e-value: 6.1E-45
score: 153.5
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 23..307
score: 22.035975
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 20..587
NoneNo IPR availablePANTHERPTHR33116:SF33OS01G0885550 PROTEINcoord: 20..587
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 36..266
e-value: 1.88385E-48
score: 166.698
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 6..288

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0041333Lag0041333gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0041333.1.exon1Lag0041333.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0041333.1cds.Lag0041333.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0041333.1Lag0041333.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009987 cellular process
biological_process GO:0071704 organic substance metabolic process
biological_process GO:0044238 primary metabolic process
biological_process GO:0050789 regulation of biological process
cellular_component GO:0043229 intracellular organelle
molecular_function GO:0005488 binding
molecular_function GO:0003824 catalytic activity