Lag0041146.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0041146.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ribonuclease H protein
Location	chr13: 12812564 .. 12815252 (+)
Sequence length	963
RNA-Seq Expression	Lag0041146.1
Synteny	Lag0041146.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGGAGATGGTGAGTGAGAGAGAAAGAAAGAAGAAGAAAAAAAGAGAAAAAAATGTCGGCGGCGGTGGGCGGCTGCCGGAGACGGCGGTGACCGGCGGGAAGCGGCGGCGCTGGAGTTTTGGTGAGAGTGTGAAGAAGAAGAGGGAGGTTGGTGATGATGGGAAGGTTGAAGAAGAAGATGAAAGAAAAAAAAAAGAAATAAAAATATATTTTAAAATTAATATTTTTACTATTTTAATATAATTTTATTTTAAAAAAATTAAAAATAATTTTTTTTATTAAAATAATGGTGCCACGTCAGCAAACTGTTAGGATTTAACGTCAAACTAGACGGCAGGGACTAAATTGCTCCTTATCTCAAACCTCAGGGGTATTTTTGTTGAGTTTGAAAACTCAGGGACCAAAGTGTAACTTAACCCAAACCTCAGGGACCAAAAGTGTATTTTTCCCATAAAAATATTATTATTATTAATTTATTTTATTTCTTTTTATTTCTCTCCTCTCTTCCACCTTCTTCTTCCTTCAGTCCTTCTATTGTTTCTTTTTCTTCAACAATATTCCATCCATTGCCGGCGGCCGGAGACCGGCGACCACCGCCGGCGGTAACTTTTTTTTTTCTTCTCTTCTCCCTCTTTTTTTCCCTCCTTCTTCAGTTTTTTTTTTAAAAAATAATAAACCGACTTCGCTGGAAAAATCTCAGAGACCAGTACCAATAGGCCATGGCATAGAAGTAAAGAACCTGAATTCTCATTCTTTATCATTCTAAAACCCCTCAGAGACGGCTTTAAGATCAGAATCACAGCCGACCAGAACCTCTAATTTTTATCTGACGAATGATTAAACCCTAAAACTCACCCACTTCAACACAAAATTTCTGCAAAAACAGAATGCATAACTTACTAAAAACTCAAATCCTATCCGTTAAGAATCTCCAACTATGATCAACAAGTGCTGCAAGAGCAAATAGTTTTTTTTTTCTTCTATAAAAAGAAGACATTCGTCTTCTTCAGATCAAGTTCGTTCCCTGCATAGGCTTGCAAAGGTATTTTATTTTTCTCTTTTTCTCCCTTTCTTTTATATATCCATCTTTCATTCTTTTATATATCCATCTTTCTTTGCCATTTTTTTCCAGCAATATCAGCTTTTCCGAGCATAGTTCTTGAGTCGGGCTTGCAAAGGTATTTTTTTTTCTCCACATATGCTATGGGGTGTTTCAGAATTTCCAAATGTATCCTCTCCAAAATCAAGGTTATATGTGTGAAGTTTTGGTGGGGTTCTTCCGGAGAGAAACGTTGAATTCATTGGAAGAAATAGGAAGATTTATGTAAGCCTAAGGAGGTGGGTGGCCTTAACTTCCAAGATCTTGAACAGTTCAATCAGGCTATGATAACTAAGCAGGCTTGGCGAATTTTAACAAAGCCTAATCTTACCCTGGCTAGAGTGCTTCGAGGTTGTTATTTCCCTTCCACTACGATTTTAAATGCAAACAACCCATCTAACTCTTCCTTCTTTTGGAAGAGCATTCGTTGGGGAGTGGAGTTGCTAAGAGGAGGCATAAGGATGAACCTAGGCAACGACCACAATATCAGAATGTCCAGTGATCCGTGGATACCTCGACCTCTAACCTTCAAGGTTTTGTCCTCAGGTGGTGTCACTGACGAGAATTTGTTGGTCGCTGAATTTTTTACCCTTCTCTCCAATGGGATGTCGGTAAGCTAGAACAATTCCTTGGGAGGGAGGATGTCGATCAAATTTTACAATTGTCTATTAGTGGAACGCGGTGCCAGATCGTTGGATTTGGCATTACAATAAGAGGGGAGAGTACACGATCAAGAGCGGTTATAAGTTAGGTATGCTCAAGGTATGCTCAACCCTCAGATAGAATCCTTGTCCTCATATGGGCCCATCAAAAGATTGGTGGAAACAACTATGACATTTGAACATTCCAAATAAGATCAAACATTTTGTGTGGAAGTCACATCATCACTTCATCCCTTCAAAGTTAAATCTTTGGCATCATCACGTTCTAGTGGAAGGTATGTGCCCACTTTGTGCAGAGAGTCCTGAAATGACGGACCACGTGCTATTCCAATGTTCATGAGCCCAACGCGTATATGGGAGGTTATGTTGCCCAACATTTTTCACACGGTGGTAGGTAACTTGGATATCTAAGATCGGTGGATCACACTTAGTCGATGTTTCAAAGAAGATTTGAACCTTATTTGTATAGATGCTTGGGCCATCTGGAACAATCGCAACAATTTCGTTCATAATTTTCCTAACATTTTGTGCAGAGGCTACTTCTTTGTTGATTGAATACGGTAAGGCCAATGCTTCATGTGGTTCTAGAGGTCGTCCACGTTCGGAGGACGGTTTCCTGTTCCAATCAAGGGGAAGTCTTATTATGAACGTCGATGCAACGTTCGACAAAATTAGTAGGAAGATCGGAGTAGGGGTTGTGATTCGAGATAACTCGGGTTCTTTAAAGGTCGTGATGTCTATGTCCTCTCATATCTGCCCTAATCCTTTATGTGCAGAGGCTACTGCTTTGTTGGAAGGTATGATGTTGGCCATAAACTTGAATATGTCAAGCATGACGACCTTATCTGACTCATTATCCTTGATTCGTATGTCAAGGATATCAGGCCCATGGGCCATTGTAAAGACAAGGATACAAGCCAAATAG

mRNA sequence

ATGAGGGAGATGGTGAGTGAGAGAGAAAGAAAGAAGAAGAAAAAAAGAGAAAAAAATGTCGGCGGCGGTGGGCGGCTGCCGGAGACGGCGGTGACCGGCGGGAAGCGGCGGCGCTGGAGTTTTGGTGAGAGTGTGAAGAAGAAGAGGGAGGTTGGTGATGATGGGAAGTTCAATCAGGCTATGATAACTAAGCAGGCTTGGCGAATTTTAACAAAGCCTAATCTTACCCTGGCTAGAGTGCTTCGAGGTTGTTATTTCCCTTCCACTACGATTTTAAATGCAAACAACCCATCTAACTCTTCCTTCTTTTGGAAGAGCATTCGTTGGGGAGTGGAGTTGCTAAGAGGAGGCATAAGGATGAACCTAGGCAACGACCACAATATCAGAATGTCCAGTGATCCGTGGATACCTCGACCTCTAACCTTCAAGGTTTTGTCCTCAGGTGGTGTCACTGACGAGAATTTGTTGGTCGCTGAATTTTTTACCCTTCTCTCCAATGGGATGTCGATGCTTGGGCCATCTGGAACAATCGCAACAATTTCGTTCATAATTTTCCTAACATTTTGTGCAGAGGCTACTTCTTTGTTGATTGAATACGGTAAGGCCAATGCTTCATGTGGTTCTAGAGGTCGTCCACGTTCGGAGGACGGTTTCCTGTTCCAATCAAGGGGAAGTCTTATTATGAACGTCGATGCAACGTTCGACAAAATTAGTAGGAAGATCGGAGTAGGGGTTGTGATTCGAGATAACTCGGGTTCTTTAAAGGTCGTGATGTCTATGTCCTCTCATATCTGCCCTAATCCTTTATGTGCAGAGGCTACTGCTTTGTTGGAAGGTATGATGTTGGCCATAAACTTGAATATGTCAAGCATGACGACCTTATCTGACTCATTATCCTTGATTCGTATGTCAAGGATATCAGGCCCATGGGCCATTGTAAAGACAAGGATACAAGCCAAATAG

Coding sequence (CDS)

ATGAGGGAGATGGTGAGTGAGAGAGAAAGAAAGAAGAAGAAAAAAAGAGAAAAAAATGTCGGCGGCGGTGGGCGGCTGCCGGAGACGGCGGTGACCGGCGGGAAGCGGCGGCGCTGGAGTTTTGGTGAGAGTGTGAAGAAGAAGAGGGAGGTTGGTGATGATGGGAAGTTCAATCAGGCTATGATAACTAAGCAGGCTTGGCGAATTTTAACAAAGCCTAATCTTACCCTGGCTAGAGTGCTTCGAGGTTGTTATTTCCCTTCCACTACGATTTTAAATGCAAACAACCCATCTAACTCTTCCTTCTTTTGGAAGAGCATTCGTTGGGGAGTGGAGTTGCTAAGAGGAGGCATAAGGATGAACCTAGGCAACGACCACAATATCAGAATGTCCAGTGATCCGTGGATACCTCGACCTCTAACCTTCAAGGTTTTGTCCTCAGGTGGTGTCACTGACGAGAATTTGTTGGTCGCTGAATTTTTTACCCTTCTCTCCAATGGGATGTCGATGCTTGGGCCATCTGGAACAATCGCAACAATTTCGTTCATAATTTTCCTAACATTTTGTGCAGAGGCTACTTCTTTGTTGATTGAATACGGTAAGGCCAATGCTTCATGTGGTTCTAGAGGTCGTCCACGTTCGGAGGACGGTTTCCTGTTCCAATCAAGGGGAAGTCTTATTATGAACGTCGATGCAACGTTCGACAAAATTAGTAGGAAGATCGGAGTAGGGGTTGTGATTCGAGATAACTCGGGTTCTTTAAAGGTCGTGATGTCTATGTCCTCTCATATCTGCCCTAATCCTTTATGTGCAGAGGCTACTGCTTTGTTGGAAGGTATGATGTTGGCCATAAACTTGAATATGTCAAGCATGACGACCTTATCTGACTCATTATCCTTGATTCGTATGTCAAGGATATCAGGCCCATGGGCCATTGTAAAGACAAGGATACAAGCCAAATAG

Protein sequence

MREMVSERERKKKKKREKNVGGGGRLPETAVTGGKRRRWSFGESVKKKREVGDDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRGGIRMNLGNDHNIRMSSDPWIPRPLTFKVLSSGGVTDENLLVAEFFTLLSNGMSMLGPSGTIATISFIIFLTFCAEATSLLIEYGKANASCGSRGRPRSEDGFLFQSRGSLIMNVDATFDKISRKIGVGVVIRDNSGSLKVVMSMSSHICPNPLCAEATALLEGMMLAINLNMSSMTTLSDSLSLIRMSRISGPWAIVKTRIQAK

Homology

[Close]

BLAST of Lag0041146.1 vs. NCBI nr
Match: XP_024178198.1 (uncharacterized protein LOC112184155 [Rosa chinensis])

HSP 1 Score: 112.1 bits (279), Expect = 9.1e-21
Identity = 81/295 (27.46%), Postives = 131/295 (44.41%), Query Frame = 0


Query: 57  FNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRG 116
           FN A++ KQ WR++ +P+  LA+VL+  YFP T  + A     +SF W+SI  G +LLR 
Sbjct: 153 FNIALLAKQGWRLICRPDSLLAQVLKARYFPQTDFMQAELHKGASFTWRSILKGRDLLRK 212

Query: 117 GIRMNLGNDHNIRMSSDPWIPRPLTFKVLSSGGVTDENLLVAEFF-----TLLSNGMSML 176
           G+R  +GN  NI + +DPW+P P  F+  S      E   VA+F          +G+  L
Sbjct: 213 GLRFQVGNGENISVWNDPWVPLPYRFRPFSLPMQGTEEFKVADFIDSEAGAFWLHGLLNL 272

Query: 177 GPSGTIATI--------------------------------------SFIIFLTFCAEAT 236
            P     T+                                      S+   +   A AT
Sbjct: 273 QPGKHPTTLLDIWFWDMIEALDGEKLEYFLMALWVVWMERNNMVWKGSYFNIINMHAWAT 332

Query: 237 SLLIEYGKANASCGSRGRPRSEDGFLFQSRGSLIMNVDATFDKISRKIGVGVVIRDNSGS 296
           S L +Y   +   G + R R+   +    RG L +N+D +F   +   GVGVV+R+  G+
Sbjct: 333 SFLDDYKNLHKRQGHKAR-RTPIKWSRPPRGRLKINIDGSFCADTGSGGVGVVVRNYEGT 392

Query: 297 LKVVMSMSSHICPNPLCAEATALLEGMMLAINLNMSSMTTLSDSLSLIRMSRISG 309
                + +     + +  EA AL  G+++AI+ +   +   SD ++L+    + G
Sbjct: 393 CMATFARTVQYAQSAIHMEAEALRAGLLIAIHQDWKEIEVESDCVNLVNALHVDG 446

[Close]

BLAST of Lag0041146.1 vs. NCBI nr
Match: XP_018837833.1 (uncharacterized protein LOC109003938 [Juglans regia] >KAF5450327.1 hypothetical protein F2P56_030688 [Juglans regia])

HSP 1 Score: 110.5 bits (275), Expect = 2.6e-20
Identity = 74/246 (30.08%), Postives = 115/246 (46.75%), Query Frame = 0


Query: 57  FNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRG 116
           FN+A++ KQ WRILT PN    +VL+  Y   T +L A+     S  W+S+   +ELL  
Sbjct: 357 FNRALLAKQCWRILTNPNSIATKVLKDKYHRKTEVLEASLGRGPSLIWRSLMGSMELLNE 416

Query: 117 GIRMNLGNDHNIRMSSDPWIPRPLTFKVLSSGGVTDENLLVAEFFTLLSNGMSMLGPSGT 176
           G+   +GN  NI++  D W+P+  ++   S     ++   VAE            G  G 
Sbjct: 417 GLVWRVGNGQNIQIWRDKWVPKQSSYMNQSPNQFLNKEAKVAELID--------EGKRGW 476

Query: 177 IATISFIIFLTFCAEATSLLIEYGKANASCGSRGRPRSEDGFLFQSRGSLIMNVDATFDK 236
              +   +     A+ TS     G A      RGR R    ++    G + ++ DA FD+
Sbjct: 477 NEDLVRKVLCEEEADLTSTNYPTGLARKIQFDRGRVR----WIAPREGEVKIDWDAAFDQ 536

Query: 237 ISRKIGVGVVIRDNSGSLKVVMSMSSHICPNPLCAEATALLEGMMLAINLNMSSMTTLSD 296
             +++G GV+IRD  G +   +SM     P+P  AE  AL   + L+  L +   T   D
Sbjct: 537 KEKRMGAGVIIRDVEGEVLAALSMPRSNIPSPGMAEVNALWRALQLSAELGIEHATFEGD 590

Query: 297 SLSLIR 303
           +L +IR
Sbjct: 597 ALEIIR 590

[Close]

BLAST of Lag0041146.1 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 109.0 bits (271), Expect = 7.7e-20
Identity = 52/111 (46.85%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 57   FNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRG 116
            FNQA++ K  WR L  PNL +++VL+  YF  T++L A+N S SS+FWK   WG +LL  
Sbjct: 954  FNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVK 1013

Query: 117  GIRMNLGNDHNIRMSSDPWIPRPLTFKVLS-SGGVTDENLLVAEFFTLLSN 167
            G+R+ +GN   I+  SDPW+PRP TFK L  + G  D    VA F T   N
Sbjct: 1014 GLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTT--VASFITADGN 1062

[Close]

BLAST of Lag0041146.1 vs. NCBI nr
Match: XP_022131662.1 (uncharacterized protein LOC111004787 [Momordica charantia])

HSP 1 Score: 102.1 bits (253), Expect = 9.4e-18
Identity = 47/118 (39.83%), Postives = 71/118 (60.17%), Query Frame = 0


Query: 35  KRRRWSFGESVKKKREVG-----DDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPST 94
           K+  W   E +   +E+G     D   FNQA++ KQ WR+L  PN+ ++RVL+  YF  +
Sbjct: 59  KKLHWMSWERMCLPKELGGLNFRDLEGFNQALVAKQVWRVLQNPNILVSRVLKAKYFHDS 118

Query: 95  TILNANNPSNSSFFWKSIRWGVELLRGGIRMNLGNDHNIRMSSDPWIPRPLTFKVLSS 148
            +L A +  N S+FWK   WG +LL  G+R  +GN   I + SDPWIPRP +F+ +++
Sbjct: 119 LVLQATHLRNQSYFWKGFIWGRDLLIKGLRSRVGNGSTINIFSDPWIPRPYSFRPITA 176

[Close]

BLAST of Lag0041146.1 vs. NCBI nr
Match: PKA49974.1 (Putative ribonuclease H protein [Apostasia shenzhenica])

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-17
Identity = 45/109 (41.28%), Postives = 70/109 (64.22%), Query Frame = 0


Query: 57  FNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRG 116
           FN+A++ KQ WRI+T  N  +ARVL+  YF +   L A    NSS+ W+SI WG +LL+ 
Sbjct: 285 FNKALLAKQGWRIITHSNSLMARVLKAKYFHNCDFLMAQASPNSSYIWRSILWGRDLLKL 344

Query: 117 GIRMNLGNDHNIRMSSDPWIPRPLTFKVLSSGGVTDENLLVAEFFTLLS 166
           G+R  +GN +++R+  DPW+PRP++F+ ++S    + NL       LL+
Sbjct: 345 GLRWRIGNGNSVRVFGDPWLPRPISFQAITS---PNPNLAATRIVELLT 390

[Close]

BLAST of Lag0041146.1 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.4e-10
Identity = 31/84 (36.90%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 53  DDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVE 112
           D G FNQA++ KQ++RI+ +P+  L+R+LR  YFP ++++  +  +  S+ W+SI  G E
Sbjct: 61  DLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRE 120

Query: 113 LLRGGIRMNLGNDHNIRMSSDPWI 137
           LL  G+   +G+  + ++  D WI
Sbjct: 121 LLSRGLLRTIGDGIHTKVWLDRWI 144

[Close]

BLAST of Lag0041146.1 vs. ExPASy TrEMBL
Match: A0A2I4G1R1 (uncharacterized protein LOC109003938 OS=Juglans regia OX=51240 GN=LOC109003938 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.3e-20
Identity = 74/246 (30.08%), Postives = 115/246 (46.75%), Query Frame = 0


Query: 57  FNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRG 116
           FN+A++ KQ WRILT PN    +VL+  Y   T +L A+     S  W+S+   +ELL  
Sbjct: 357 FNRALLAKQCWRILTNPNSIATKVLKDKYHRKTEVLEASLGRGPSLIWRSLMGSMELLNE 416

Query: 117 GIRMNLGNDHNIRMSSDPWIPRPLTFKVLSSGGVTDENLLVAEFFTLLSNGMSMLGPSGT 176
           G+   +GN  NI++  D W+P+  ++   S     ++   VAE            G  G 
Sbjct: 417 GLVWRVGNGQNIQIWRDKWVPKQSSYMNQSPNQFLNKEAKVAELID--------EGKRGW 476

Query: 177 IATISFIIFLTFCAEATSLLIEYGKANASCGSRGRPRSEDGFLFQSRGSLIMNVDATFDK 236
              +   +     A+ TS     G A      RGR R    ++    G + ++ DA FD+
Sbjct: 477 NEDLVRKVLCEEEADLTSTNYPTGLARKIQFDRGRVR----WIAPREGEVKIDWDAAFDQ 536

Query: 237 ISRKIGVGVVIRDNSGSLKVVMSMSSHICPNPLCAEATALLEGMMLAINLNMSSMTTLSD 296
             +++G GV+IRD  G +   +SM     P+P  AE  AL   + L+  L +   T   D
Sbjct: 537 KEKRMGAGVIIRDVEGEVLAALSMPRSNIPSPGMAEVNALWRALQLSAELGIEHATFEGD 590

Query: 297 SLSLIR 303
           +L +IR
Sbjct: 597 ALEIIR 590

[Close]

BLAST of Lag0041146.1 vs. ExPASy TrEMBL
Match: A0A803Q154 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.8e-20
Identity = 48/109 (44.04%), Postives = 73/109 (66.97%), Query Frame = 0


Query: 38  RWSFGESVKKKREVG--DDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNAN 97
           +W++    K+K  +G  D G FNQA++ KQ WR L  P L  +RVL+ CY+P  ++L+A 
Sbjct: 308 KWNYMCKPKEKGGLGFRDLGLFNQALLAKQVWRCLRSPTLLSSRVLKACYYPGVSVLDAK 367

Query: 98  NPSNSSFFWKSIRWGVELLRGGIRMNLGNDHNIRMSSDPWIPRPLTFKV 145
             S+SSF W+S+ WG +LL  G R  +G+ + +R+ +DPW+PRP+TF V
Sbjct: 368 CGSHSSFMWRSMMWGKKLLLKGYRWRIGDGNGVRVLNDPWLPRPVTFCV 416

[Close]

BLAST of Lag0041146.1 vs. ExPASy TrEMBL
Match: A0A803PQQ6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.8e-20
Identity = 48/109 (44.04%), Postives = 72/109 (66.06%), Query Frame = 0


Query: 38  RWSFGESVKKKREVG--DDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNAN 97
           +W++    K+K  +G  D G FNQA++ KQ WR L  P L  +RVL+ CY+P   +L+A 
Sbjct: 74  KWNYMCKQKEKGGLGFRDLGLFNQALLAKQVWRCLRCPTLLSSRVLKACYYPGVDVLDAK 133

Query: 98  NPSNSSFFWKSIRWGVELLRGGIRMNLGNDHNIRMSSDPWIPRPLTFKV 145
             S+SSF W+S+ WG +LL  G R  +GN + +++  DPW+PRP+TF+V
Sbjct: 134 CGSHSSFMWRSMMWGKKLLLKGYRWRIGNGNGVQVLDDPWLPRPVTFRV 182

[Close]

BLAST of Lag0041146.1 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 3.7e-20
Identity = 52/111 (46.85%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 57   FNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRG 116
            FNQA++ K  WR L  PNL +++VL+  YF  T++L A+N S SS+FWK   WG +LL  
Sbjct: 954  FNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSKSSYFWKGFLWGRDLLVK 1013

Query: 117  GIRMNLGNDHNIRMSSDPWIPRPLTFKVLS-SGGVTDENLLVAEFFTLLSN 167
            G+R+ +GN   I+  SDPW+PRP TFK L  + G  D    VA F T   N
Sbjct: 1014 GLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTT--VASFITADGN 1062

[Close]

BLAST of Lag0041146.1 vs. ExPASy TrEMBL
Match: A0A803P9M1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 9.2e-19
Identity = 82/280 (29.29%), Postives = 133/280 (47.50%), Query Frame = 0


Query: 35   KRRRWSFGESVKKKREVG-----DDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPST 94
            K+  W     + K ++ G     D G FNQA++ KQ WR +  PN   +RVL+  YFP+ 
Sbjct: 973  KKIHWCKWAQLCKPKDKGGLGFRDLGFFNQALLAKQVWRCIRYPNNLCSRVLKASYFPNK 1032

Query: 95   TILNANNPSNSSFFWKSIRWGVELLRGGIRMNLGNDHNIRMSSDPWIPRPLTFKVLSSGG 154
            +IL      ++SF W+S+ WG +++  G R  +GN  N+R+  DPW+PRP+TFK+     
Sbjct: 1033 SILETKCGVHASFVWRSLMWGKKVIVNGYRWRVGNGENVRVLEDPWLPRPVTFKIYDKPP 1092

Query: 155  VTDENLLVAEF---------------FTLLSNGMSMLGPS-------GTIATISFIIFLT 214
            ++ ENL VA+                F +    + +  PS       G  A I+  + + 
Sbjct: 1093 LS-ENLFVADLKLVDGSWDKDFIRAVFNMEDAELILSMPSMGNNVVHGGAAPIAGDM-VD 1152

Query: 215  FCAEATSLLIEYGKANASCGSRGRPRSEDGFLFQSRGSLIMNVDATFDKISRKIGVGVVI 274
            +C    + L+E+       GSR +  +   +     G + +NVDA   K    +G+G V 
Sbjct: 1153 WC---KNYLVEFQGGGDIKGSRNQHETSK-WGAHEMGRVKINVDAGV-KGGGGVGIGCVA 1212

Query: 275  RDNSGSLKVVMSMSSHICPNPLCAEATALLEGMMLAINLN 288
            RD+ G  +   S+  +    PL  E  A+L G+    + N
Sbjct: 1213 RDSRGRSQFASSIVINREIAPLQLELRAILLGLRWEFSAN 1245

[Close]

BLAST of Lag0041146.1 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.4e-11
Identity = 31/84 (36.90%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 53  DDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNANNPSNSSFFWKSIRWGVE 112
           D G FNQA++ KQ++RI+ +P+  L+R+LR  YFP ++++  +  +  S+ W+SI  G E
Sbjct: 61  DLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRE 120

Query: 113 LLRGGIRMNLGNDHNIRMSSDPWI 137
           LL  G+   +G+  + ++  D WI
Sbjct: 121 LLSRGLLRTIGDGIHTKVWLDRWI 144

[Close]

BLAST of Lag0041146.1 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 9.1e-11
Identity = 36/102 (35.29%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 37  RRWSFGESVKKKREVG--DDGKFNQAMITKQAWRILTKPNLTLARVLRGCYFPSTTILNA 96
           + W      K +  +G  D   FN A++ KQ WR+L++P   +A+V +  YF  +  LNA
Sbjct: 42  KAWDHLSCYKAEGGIGFKDIEAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNA 101

Query: 97  NNPSNSSFFWKSIRWGVELLRGGIRMNLGNDHNIRMSSDPWI 137
              S  SF WKSI    E+LR G R  +GN  +I +    W+
Sbjct: 102 PLGSRPSFVWKSIHASQEILRQGARAVVGNGEDIIIWRHKWL 143

[Close]

BLAST of Lag0041146.1 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 8.2e-04
Identity = 22/64 (34.38%), Postives = 34/64 (53.12%), Query Frame = 0


Query: 81  LRGCYFPSTTILNANNPSNSSFFWKSIRWGVELLRGGIRMNLGNDHNIRMSSDPWI---- 140
           ++  YF   +IL+A      S+ W S+  G+ LL+ G R  +G+  NIR+  D  +    
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHP 60

The following BLAST results are available for this feature:

BLAST of Lag0041146.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_024178198.1	9.1e-21	27.46	uncharacterized protein LOC112184155 [Rosa chinensis]	[more]
XP_018837833.1	2.6e-20	30.08	uncharacterized protein LOC109003938 [Juglans regia] >KAF5450327.1 hypothetical ...	[more]
XP_022158377.1	7.7e-20	46.85	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_022131662.1	9.4e-18	39.83	uncharacterized protein LOC111004787 [Momordica charantia]	[more]
PKA49974.1	1.2e-17	41.28	Putative ribonuclease H protein [Apostasia shenzhenica]	[more]

BLAST of Lag0041146.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93295	3.4e-10	36.90	Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Lag0041146.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2I4G1R1	1.3e-20	30.08	uncharacterized protein LOC109003938 OS=Juglans regia OX=51240 GN=LOC109003938 P...	[more]
A0A803Q154	2.8e-20	44.04	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803PQQ6	2.8e-20	44.04	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A6J1DX30	3.7e-20	46.85	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A803P9M1	9.2e-19	29.29	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Lag0041146.1 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00310.1	2.4e-11	36.90	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]
AT4G29090.1	9.1e-11	35.29	Ribonuclease H-like superfamily protein	[more]
AT3G09510.1	8.2e-04	34.38	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 229..303 e-value: 8.0E-10 score: 38.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..42
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 228..303
None	No IPR available	PANTHER	PTHR47074:SF25	SUBFAMILY NOT NAMED	coord: 228..303
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 229..320 e-value: 1.29807E-9 score: 53.472

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0041146	Lag0041146	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0041146.1.exon1	Lag0041146.1.exon1	exon
Lag0041146.1.exon2	Lag0041146.1.exon2	exon
Lag0041146.1.exon3	Lag0041146.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0041146.1	cds.Lag0041146.1	CDS
cds.Lag0041146.1	cds.Lag0041146.1_2	CDS
cds.Lag0041146.1	cds.Lag0041146.1_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0041146.1	Lag0041146.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity