Lag0041015.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0041015.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionphosphoglucan phosphatase LSF2, chloroplastic
Locationchr13: 10799857 .. 10802136 (-)
Sequence length849
RNA-Seq ExpressionLag0041015.1
SyntenyLag0041015.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATGGGAGTTTTTAGCCTCGATCGTCTTTCATCGCTGTTTGCAGTTTCTCCACTCCAGAATCATGCGCACAATAAATCTTTACCATTTTGTTCAAAGCTTTCAAACCCCCACTTCCGATCGAACAAGATTTGGTGCGGAATGCCTGAGAGTGGAATTCGGCAAAACCCCACAAGCGGCAGAGCTTCCACCTCCAAGAACAGGATGGAGGAATACAATTTGGCTATGAAGAAGATGATGAGAAACCCCTATGAGTATCACCATGAACTGGGTCAGTTTCTTCTTTTCTTCATCCTGTTGTTTGATTTGTTCCCTTTTAGTGTTTGAATTCTTGGCCATTTTATATGCTTCAATCGCTCTGCTTCTGCAATTCGTTAATGGATAATTTTGGGTCATTTGTGTTGTTTGGAATTAAAAGTGAATGGGTGATGGGATTGAAGTGAATCTATTGGAGTTGAGTAGTTGATGTATTGAAGTGCTTTTGTTGTTTTTTGGTTTGATATGTGCACAGTTGAGCTTTTTGATGATTTATGGATACCTTTTGCTAAAAGCTAATTGTGCTTGAAGTAATTAGAGGGCCAAGATCTTTATCGACCTATAAACATTGATAAGCTCTTTGAATTGTTTGCTATGTCCGAAAACATTGTGTGTCATCTCTTTTGTTGGGATGAAAGAGCTTCAGTTTATGAAACCTTATATTGGAAGCAGTTTGGCTGGTCACTAAGGTCCCGTTTGGTTCCATTATATGAGATAAGTCGACAGAAAACAAATGCTTTTGGTGTGTTTCTTGAACTTTATGGATTTGTTTAGAATTAATTCTTGAATTCAATATTGGAAAATGCTGTTTGGTAAGAAAGAAACTGTAAGAGAGAAACTGCTTTTGAGAAAACACAGTGTTCTATTTTTCAAGAAAGCTCTTTCTGTATTCCTTTGGCTTCAGTGGACTAATGTTTTTTAAAATTGTTTTCAAATTCACCCCTACCAAACACAAATTAAGTATGATCATGGCATCCCTTGTTTTCAAATGCCTAAAATGATAAACGGATTCAATACAAAACCGGTCCTAAGTAAACTTTCTAGCTAGCATTCTGACAGACAGAACTTGAAGATATCAGTTTGCTGAAAATTGATGTTCCCATAATGCTCCTGCAATCAGGTATGAACTATACTCTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCGAAGATATTGATTTCTTGAAAGAGGAAGAAGGTGTTGCCTATATTCTAAATTTACAGCAGGACAAGGATGTCGAGTATTGGGGAATCAACTTACAATCCATTATAGATAGATGTAAAGAACTTGGAATTCATCATATGAGGCAACCGGTAAGCACGTATCCTTTTAATACTCTGTGAGCTTAAATATTTGGTAGAGCCATTTTCAAATACACTGATTTCTCTCCCTTTCTCTGTTTGTCTCATATCTTTTAACTGTTTTATATACTTCATGAAATGGGAAAGCTTTTTCCGATTAAGTTTTTCTTTTGAGCTTAAGGAGAGGAAAGGAATGGAAAAAGAACACGAAAGAAGTGATTAGCTAGTTTCTCTTGTAGCAACTTACTCTGAAGTTCTGAACCTCGTGAATTTGTTTGCTTTTTTGTTCTTCGCCTACTCATTGGGTTAACCTGTTCTTTCTTAGGCTAAAGATTTTGATCCAGATTCCTTGAGAAATGGATTGCCTAAAGCTGTTTCCTTGCTAGAATGGGCGATTTCAGAGGGCAAAGGAAAAGTTTATGTGCACTGCACCGCCGGACTGGGAAGGGCCCCTGCCGTTGCAATAGCGTACCTGTACTGGTTTTGCGGAATGAATGCAAGTGCTTTTTTACTTTAAAACTTATGTTTTACATTTAAGATTCCTGCCATTGACAAGTTTTGAAGCTGGAAAAATAATAATGATAATAGGATAATTGATATCAAGATTGCATTTCTAAACTACCATCACATGTATGTTGATATTTTCTGATTTGCATGGACTTTTCACAACTTCTCCCAATAAAGTTACCAACTTATTGGATTCTTATTTTGGCTATATGACAGTTAAATACAGCATATGAAGCCCTGACTTCAAAAAGACCTTGTGGACCAAGTAAAAAAGCGATCCGCGGAGCCACATATGATCTATCTAAAAATGATCCATGGAAAGAGCCATTTGAGAGCCTCCCAGATAATGCATTCGAGGATATTGCAGATTGGGAAAGAAACTTGATTCAGGATCGGATTCGTTCTCTTCGTGGAACTTAG

mRNA sequence

ATGAAAATGGGAGTTTTTAGCCTCGATCGTCTTTCATCGCTGTTTGCAGTTTCTCCACTCCAGAATCATGCGCACAATAAATCTTTACCATTTTGTTCAAAGCTTTCAAACCCCCACTTCCGATCGAACAAGATTTGGTGCGGAATGCCTGAGAGTGGAATTCGGCAAAACCCCACAAGCGGCAGAGCTTCCACCTCCAAGAACAGGATGGAGGAATACAATTTGGCTATGAAGAAGATGATGAGAAACCCCTATGAGTATCACCATGAACTGGGTATGAACTATACTCTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCGAAGATATTGATTTCTTGAAAGAGGAAGAAGGTGTTGCCTATATTCTAAATTTACAGCAGGACAAGGATGTCGAGTATTGGGGAATCAACTTACAATCCATTATAGATAGATGTAAAGAACTTGGAATTCATCATATGAGGCAACCGGCTAAAGATTTTGATCCAGATTCCTTGAGAAATGGATTGCCTAAAGCTGTTTCCTTGCTAGAATGGGCGATTTCAGAGGGCAAAGGAAAAGTTTATGTGCACTGCACCGCCGGACTGGGAAGGGCCCCTGCCGTTGCAATAGCGTACCTGTACTGGTTTTGCGGAATGAATGCAACATATGAAGCCCTGACTTCAAAAAGACCTTGTGGACCAAGTAAAAAAGCGATCCGCGGAGCCACATATGATCTATCTAAAAATGATCCATGGAAAGAGCCATTTGAGAGCCTCCCAGATAATGCATTCGAGGATATTGCAGATTGGGAAAGAAACTTGATTCAGGATCGGATTCGTTCTCTTCGTGGAACTTAG

Coding sequence (CDS)

ATGAAAATGGGAGTTTTTAGCCTCGATCGTCTTTCATCGCTGTTTGCAGTTTCTCCACTCCAGAATCATGCGCACAATAAATCTTTACCATTTTGTTCAAAGCTTTCAAACCCCCACTTCCGATCGAACAAGATTTGGTGCGGAATGCCTGAGAGTGGAATTCGGCAAAACCCCACAAGCGGCAGAGCTTCCACCTCCAAGAACAGGATGGAGGAATACAATTTGGCTATGAAGAAGATGATGAGAAACCCCTATGAGTATCACCATGAACTGGGTATGAACTATACTCTGATAGCTGACAATTTGATTGTCGGTTCCCAGCCTCAAAAACCCGAAGATATTGATTTCTTGAAAGAGGAAGAAGGTGTTGCCTATATTCTAAATTTACAGCAGGACAAGGATGTCGAGTATTGGGGAATCAACTTACAATCCATTATAGATAGATGTAAAGAACTTGGAATTCATCATATGAGGCAACCGGCTAAAGATTTTGATCCAGATTCCTTGAGAAATGGATTGCCTAAAGCTGTTTCCTTGCTAGAATGGGCGATTTCAGAGGGCAAAGGAAAAGTTTATGTGCACTGCACCGCCGGACTGGGAAGGGCCCCTGCCGTTGCAATAGCGTACCTGTACTGGTTTTGCGGAATGAATGCAACATATGAAGCCCTGACTTCAAAAAGACCTTGTGGACCAAGTAAAAAAGCGATCCGCGGAGCCACATATGATCTATCTAAAAATGATCCATGGAAAGAGCCATTTGAGAGCCTCCCAGATAATGCATTCGAGGATATTGCAGATTGGGAAAGAAACTTGATTCAGGATCGGATTCGTTCTCTTCGTGGAACTTAG

Protein sequence

MKMGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGRASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEGVAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNATYEALTSKRPCGPSKKAIRGATYDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT
Homology
BLAST of Lag0041015.1 vs. NCBI nr
Match: XP_022942267.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 515.8 bits (1327), Expect = 2.4e-142
Identity = 251/282 (89.01%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  SL   SSLFAV P  N+AHNKSLPFCS  S P FR NKI CG+PESGIRQNP+S R
Sbjct: 1   MGASSLACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
           AS+SKNRMEEYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDIDFLKEEEG
Sbjct: 61  ASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGI+LQS+I+RCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AISEGKGK YVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSKKAIRGAT
Sbjct: 181 AISEGKGKAYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDP KEPFESLPDNAFEDIADWERNLIQDR+RSLR T
Sbjct: 241 YDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of Lag0041015.1 vs. NCBI nr
Match: XP_022984858.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 515.4 bits (1326), Expect = 3.1e-142
Identity = 251/282 (89.01%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  SL   SSLFAV P  N+AHNKSLPFCS  S P FR NKI CG+PESGIRQNP+S R
Sbjct: 1   MGASSLACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
            S+SKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG
Sbjct: 61  TSSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGI+LQS+I+RCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSKKAIRGAT
Sbjct: 181 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDP KEPFESLPDNAFEDIADWERNLIQDR+RSL+ T
Sbjct: 241 YDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLQRT 282

BLAST of Lag0041015.1 vs. NCBI nr
Match: XP_023542341.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.5 bits (1321), Expect = 1.2e-141
Identity = 250/282 (88.65%), Postives = 259/282 (91.84%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  SL   SSLFAV P  N+AHNKSLPFCS  S P FR NKI CG+PESGIRQ P+S R
Sbjct: 1   MGASSLACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQTPSSSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
           AS+SKNRMEEYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDIDFLKEEEG
Sbjct: 61  ASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGI+LQS+I+RCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AISEGKGK YVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSKKAIRGAT
Sbjct: 181 AISEGKGKAYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDP KEPFESLPDNAFEDIADWERNLIQDR+RSLR T
Sbjct: 241 YDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of Lag0041015.1 vs. NCBI nr
Match: XP_011655031.1 (phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >XP_011655032.1 phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >KGN50717.1 hypothetical protein Csa_005846 [Cucumis sativus])

HSP 1 Score: 498.4 bits (1282), Expect = 4.0e-137
Identity = 240/282 (85.11%), Postives = 259/282 (91.84%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  +L   SSLF V PLQN+A NK LPF S LS P +R N+I CG+PESGI+QNP++ R
Sbjct: 1   MGAANLPCFSSLFGVFPLQNNAPNKFLPFSSILSKPLYRFNRICCGVPESGIQQNPSTSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
            S+SKNRME+YNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDI+FLKEEEG
Sbjct: 61  PSSSKNRMEDYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGI+LQSII+RCKELGIHHMRQPAKDFDPDSLR GLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGIDLQSIIERCKELGIHHMRQPAKDFDPDSLRTGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AIS+GKGKVYVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSK+AIRGAT
Sbjct: 181 AISKGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDPWKEPFESLPDNAFEDIADWERNLI+DR+R+LR T
Sbjct: 241 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIRDRVRALRRT 282

BLAST of Lag0041015.1 vs. NCBI nr
Match: XP_008456918.1 (PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis melo] >XP_008456919.1 PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 498.0 bits (1281), Expect = 5.2e-137
Identity = 241/282 (85.46%), Postives = 257/282 (91.13%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  +L   SSLF V PLQN+A NK LPF S LS P +R N+I CG+PESGI+QNP++ R
Sbjct: 1   MGAANLPCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
            S+SKNRM+EYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDI+FLKEEEG
Sbjct: 61  PSSSKNRMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGINLQSII+RCKELGI HMRQPAKDFDPDSLR GLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AIS+GKGKVYVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSK+AIRGAT
Sbjct: 181 AISKGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDPWKEPFESLPDNAFEDIADWERNLI DR+RSLR T
Sbjct: 241 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIWDRVRSLRRT 282

BLAST of Lag0041015.1 vs. ExPASy Swiss-Prot
Match: Q9SRK5 (Phosphoglucan phosphatase LSF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF2 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 5.4e-105
Identity = 177/230 (76.96%), Postives = 206/230 (89.57%), Query Frame = 0

Query: 56  QNP-TSGRASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDI 115
           +NP T+G + +SKN+ME+YN AMK++MR+PYEYHH+LGMNYTLI D LIVGSQPQKPEDI
Sbjct: 53  ENPGTNGVSLSSKNKMEDYNTAMKRLMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDI 112

Query: 116 DFLKEEEGVAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLP 175
           D LK+E+ VAYILNLQQDKD+EYWGI+L SI+ RCKELGI HMR+PAKDFDP SLR+ LP
Sbjct: 113 DHLKQEQNVAYILNLQQDKDIEYWGIDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLP 172

Query: 176 KAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPS 235
           KAVS LEWA+SEGKG+VYVHC+AGLGRAP V+IAY+YWFC M  N  Y+ L SKRPCGP+
Sbjct: 173 KAVSSLEWAVSEGKGRVYVHCSAGLGRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPN 232

Query: 236 KKAIRGATYDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           K AIRGATYDL+KNDPWKEPFESLP+NAFEDIADWER LIQ+R+R+LRGT
Sbjct: 233 KGAIRGATYDLAKNDPWKEPFESLPENAFEDIADWERKLIQERVRALRGT 282

BLAST of Lag0041015.1 vs. ExPASy Swiss-Prot
Match: Q9FEB5 (Phosphoglucan phosphatase DSP4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.9e-34
Identity = 96/260 (36.92%), Postives = 134/260 (51.54%), Query Frame = 0

Query: 16  AVSPLQNHA-----HNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGRAS---TSK 75
           +VSPL         H+ S      L +P  ++N     +    + ++ +S   S     +
Sbjct: 11  SVSPLLGFGCIQRDHSSSSSSLKMLISPPIKANDPKSRLVLHAVSESKSSSEMSGVAKDE 70

Query: 76  NRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEGVAYIL 135
            + +EY+  M + M     Y HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I 
Sbjct: 71  EKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIF 130

Query: 136 NLQQDKDVEYWGINLQSIIDRCKELG-IHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISE 195
            LQQD D+EY+G+++ SI    K+   I H+R   +DFD   LR  LP  V  L  A+  
Sbjct: 131 CLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKR 190

Query: 196 GKGKVYVHCTAGLGRAPAVAIAYLYWFCG--MNATYEALTSKRPCGPSKKAIRGATYDLS 255
             G  YVHCTAG+GRAPAVA+ Y++W  G  +   ++ L SKR C P   AIR AT D+ 
Sbjct: 191 NGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDIL 250

Query: 256 KNDPWKEPFESLPDNAFEDI 265
                K    +L D  F  +
Sbjct: 251 TGLKRKTVTLTLKDKGFSRV 269

BLAST of Lag0041015.1 vs. ExPASy Swiss-Prot
Match: G4LTX4 (Phosphoglucan phosphatase DSP4, amyloplastic OS=Castanea sativa OX=21020 GN=DSP4 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 7.2e-33
Identity = 95/247 (38.46%), Postives = 132/247 (53.44%), Query Frame = 0

Query: 5   VFSLDRLSSLFAVSPLQNHAHNKSLPFCS-----KLSNPHFRSNKIWCGMPESGIRQNPT 64
           V +L R S+L    PLQ+   ++  P CS      +SN +   +     +  SG   +  
Sbjct: 4   VQNLPRSSAL----PLQSFKSHQRRPPCSVNTLGVMSNVNLHRSMAVKAI--SGPTSSAE 63

Query: 65  SGRASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKE 124
           +  A+  + + E Y+  M + M     Y HELG+NY  I  +LIVGS  Q PED+D L+ 
Sbjct: 64  TSDANVEEEKSETYSTNMTEAMGAVLTYRHELGINYNFIRPDLIVGSCLQTPEDVDKLR- 123

Query: 125 EEGVAYILNLQQDKDVEYWGINLQSIIDRCKEL-GIHHMRQPAKDFDPDSLRNGLPKAVS 184
             GV  I  LQQ+ D+EY+G+++  I +  K    I H+R   +DFD   LR  LP  VS
Sbjct: 124 SIGVKTIFCLQQNSDLEYFGVDINGIREYAKTYDDIQHLRAEIRDFDAFDLRVRLPAVVS 183

Query: 185 LLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCG--MNATYEALTSKRPCGPSKKAI 244
            L  AI+   G  YVHCTAGLGRAPAV +AY++W  G  +   +  L SKR C P   AI
Sbjct: 184 KLYKAINRNGGVTYVHCTAGLGRAPAVTLAYMFWVQGYKLIEAHNLLQSKRSCFPKLDAI 243

BLAST of Lag0041015.1 vs. ExPASy Swiss-Prot
Match: F4J117 (Phosphoglucan phosphatase LSF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSF1 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 6.1e-24
Identity = 68/188 (36.17%), Postives = 97/188 (51.60%), Query Frame = 0

Query: 58  PTSGRASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFL 117
           P  G        +EEY  A+ +  +    Y+H LGM Y+ I + + VGS  Q  ED++ L
Sbjct: 256 PGDGEWGYGNFPLEEYIKALDR-SKGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL 315

Query: 118 KEEEGVAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAV 177
             E G+  ILN Q   + + WGI+ QSI D C++  +  +  P KD D   LR  LP  V
Sbjct: 316 -SEAGITAILNFQGGTEAQNWGIDSQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCV 375

Query: 178 SLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLYWF--CGMNATYEALTSKRPCGPSKKA 237
            LL   + +   +V+V CT G  R+ A  IAYL+W     ++A Y  +T    C P + A
Sbjct: 376 GLLLRLLKKNH-RVFVTCTTGFDRSSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPA 435

Query: 238 IRGATYDL 244
           I  AT+DL
Sbjct: 436 IAWATWDL 440

BLAST of Lag0041015.1 vs. ExPASy Swiss-Prot
Match: Q1M199 (Laforin OS=Canis lupus familiaris OX=9615 GN=EPM2A PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.0e-10
Identity = 49/148 (33.11%), Postives = 73/148 (49.32%), Query Frame = 0

Query: 93  MNYTLIADNLIVGSQPQKPEDIDF-LKEEEGVAYILNLQQDKDV--EYWGINL------- 152
           M+Y+ I  N+ +GS P++ E I   LK E G+  ++N Q + D+     G N        
Sbjct: 155 MHYSRILPNIWLGSCPRQVEHITIKLKHELGITAVMNFQTEWDIVQNSSGCNRYPEPMTP 214

Query: 153 QSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISEGKGKVYVHCTAGLGRA 212
            ++I   KE G+ ++  P  D   +     LP+AV LL  A+ E    VYVHC AG+GR+
Sbjct: 215 DTMIKLYKEEGLVYIWMPTPDMSTEGRVQMLPQAVCLLH-ALLENGHTVYVHCNAGVGRS 274

Query: 213 PAVAIAYLYWFCGMN--ATYEALTSKRP 229
            A    +L +  G N       L +KRP
Sbjct: 275 TAAVCGWLQYVMGWNLRKVQYFLMAKRP 301

BLAST of Lag0041015.1 vs. ExPASy TrEMBL
Match: A0A6J1FPR7 (phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447363 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.2e-142
Identity = 251/282 (89.01%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  SL   SSLFAV P  N+AHNKSLPFCS  S P FR NKI CG+PESGIRQNP+S R
Sbjct: 1   MGASSLACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
           AS+SKNRMEEYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDIDFLKEEEG
Sbjct: 61  ASSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDIDFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGI+LQS+I+RCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AISEGKGK YVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSKKAIRGAT
Sbjct: 181 AISEGKGKAYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDP KEPFESLPDNAFEDIADWERNLIQDR+RSLR T
Sbjct: 241 YDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLRRT 282

BLAST of Lag0041015.1 vs. ExPASy TrEMBL
Match: A0A6J1JBS6 (phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483010 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.5e-142
Identity = 251/282 (89.01%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  SL   SSLFAV P  N+AHNKSLPFCS  S P FR NKI CG+PESGIRQNP+S R
Sbjct: 1   MGASSLACFSSLFAVFPSHNNAHNKSLPFCSNPSKPLFRLNKIRCGLPESGIRQNPSSSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
            S+SKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG
Sbjct: 61  TSSSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGI+LQS+I+RCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGIDLQSVIERCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSKKAIRGAT
Sbjct: 181 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKKAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDP KEPFESLPDNAFEDIADWERNLIQDR+RSL+ T
Sbjct: 241 YDLSKNDPGKEPFESLPDNAFEDIADWERNLIQDRVRSLQRT 282

BLAST of Lag0041015.1 vs. ExPASy TrEMBL
Match: A0A0A0KQM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218830 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.9e-137
Identity = 240/282 (85.11%), Postives = 259/282 (91.84%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  +L   SSLF V PLQN+A NK LPF S LS P +R N+I CG+PESGI+QNP++ R
Sbjct: 1   MGAANLPCFSSLFGVFPLQNNAPNKFLPFSSILSKPLYRFNRICCGVPESGIQQNPSTSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
            S+SKNRME+YNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDI+FLKEEEG
Sbjct: 61  PSSSKNRMEDYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGI+LQSII+RCKELGIHHMRQPAKDFDPDSLR GLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGIDLQSIIERCKELGIHHMRQPAKDFDPDSLRTGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AIS+GKGKVYVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSK+AIRGAT
Sbjct: 181 AISKGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDPWKEPFESLPDNAFEDIADWERNLI+DR+R+LR T
Sbjct: 241 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIRDRVRALRRT 282

BLAST of Lag0041015.1 vs. ExPASy TrEMBL
Match: A0A1S3C4E0 (phosphoglucan phosphatase LSF2, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.5e-137
Identity = 241/282 (85.46%), Postives = 257/282 (91.13%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  +L   SSLF V PLQN+A NK LPF S LS P +R N+I CG+PESGI+QNP++ R
Sbjct: 1   MGAANLPCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
            S+SKNRM+EYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDI+FLKEEEG
Sbjct: 61  PSSSKNRMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGINLQSII+RCKELGI HMRQPAKDFDPDSLR GLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AIS+GKGKVYVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSK+AIRGAT
Sbjct: 181 AISKGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           YDLSKNDPWKEPFESLPDNAFEDIADWERNLI DR+RSLR T
Sbjct: 241 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIWDRVRSLRRT 282

BLAST of Lag0041015.1 vs. ExPASy TrEMBL
Match: A0A1S3C3W8 (phosphoglucan phosphatase LSF2, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496723 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.9e-135
Identity = 236/277 (85.20%), Postives = 253/277 (91.34%), Query Frame = 0

Query: 3   MGVFSLDRLSSLFAVSPLQNHAHNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGR 62
           MG  +L   SSLF V PLQN+A NK LPF S LS P +R N+I CG+PESGI+QNP++ R
Sbjct: 1   MGAANLPCFSSLFGVFPLQNNAPNKLLPFSSTLSKPLYRFNRICCGVPESGIQQNPSTSR 60

Query: 63  ASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEG 122
            S+SKNRM+EYNLAMKKMMRNPYEYHHELGMNYT+IADNLIVGSQPQKPEDI+FLKEEEG
Sbjct: 61  PSSSKNRMDEYNLAMKKMMRNPYEYHHELGMNYTVIADNLIVGSQPQKPEDINFLKEEEG 120

Query: 123 VAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAVSLLEW 182
           VAYILNLQQDKDVEYWGINLQSII+RCKELGI HMRQPAKDFDPDSLR GLPKAVSLLEW
Sbjct: 121 VAYILNLQQDKDVEYWGINLQSIIERCKELGIRHMRQPAKDFDPDSLRTGLPKAVSLLEW 180

Query: 183 AISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPSKKAIRGAT 242
           AIS+GKGKVYVHCTAGLGRAPAVAIAYLYWFCGM  N  YEALTSKRPCGPSK+AIRGAT
Sbjct: 181 AISKGKGKVYVHCTAGLGRAPAVAIAYLYWFCGMNLNTAYEALTSKRPCGPSKRAIRGAT 240

Query: 243 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIR 278
           YDLSKNDPWKEPFESLPDNAFEDIADWERNLI DR++
Sbjct: 241 YDLSKNDPWKEPFESLPDNAFEDIADWERNLIWDRVQ 277

BLAST of Lag0041015.1 vs. TAIR 10
Match: AT3G10940.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 382.1 bits (980), Expect = 3.9e-106
Identity = 177/230 (76.96%), Postives = 206/230 (89.57%), Query Frame = 0

Query: 56  QNP-TSGRASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDI 115
           +NP T+G + +SKN+ME+YN AMK++MR+PYEYHH+LGMNYTLI D LIVGSQPQKPEDI
Sbjct: 53  ENPGTNGVSLSSKNKMEDYNTAMKRLMRSPYEYHHDLGMNYTLIRDELIVGSQPQKPEDI 112

Query: 116 DFLKEEEGVAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLP 175
           D LK+E+ VAYILNLQQDKD+EYWGI+L SI+ RCKELGI HMR+PAKDFDP SLR+ LP
Sbjct: 113 DHLKQEQNVAYILNLQQDKDIEYWGIDLDSIVRRCKELGIRHMRRPAKDFDPLSLRSQLP 172

Query: 176 KAVSLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLYWFCGM--NATYEALTSKRPCGPS 235
           KAVS LEWA+SEGKG+VYVHC+AGLGRAP V+IAY+YWFC M  N  Y+ L SKRPCGP+
Sbjct: 173 KAVSSLEWAVSEGKGRVYVHCSAGLGRAPGVSIAYMYWFCDMNLNTAYDTLVSKRPCGPN 232

Query: 236 KKAIRGATYDLSKNDPWKEPFESLPDNAFEDIADWERNLIQDRIRSLRGT 283
           K AIRGATYDL+KNDPWKEPFESLP+NAFEDIADWER LIQ+R+R+LRGT
Sbjct: 233 KGAIRGATYDLAKNDPWKEPFESLPENAFEDIADWERKLIQERVRALRGT 282

BLAST of Lag0041015.1 vs. TAIR 10
Match: AT3G52180.1 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.1e-35
Identity = 96/260 (36.92%), Postives = 134/260 (51.54%), Query Frame = 0

Query: 16  AVSPLQNHA-----HNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGRAS---TSK 75
           +VSPL         H+ S      L +P  ++N     +    + ++ +S   S     +
Sbjct: 11  SVSPLLGFGCIQRDHSSSSSSLKMLISPPIKANDPKSRLVLHAVSESKSSSEMSGVAKDE 70

Query: 76  NRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEGVAYIL 135
            + +EY+  M + M     Y HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I 
Sbjct: 71  EKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIF 130

Query: 136 NLQQDKDVEYWGINLQSIIDRCKELG-IHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISE 195
            LQQD D+EY+G+++ SI    K+   I H+R   +DFD   LR  LP  V  L  A+  
Sbjct: 131 CLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKR 190

Query: 196 GKGKVYVHCTAGLGRAPAVAIAYLYWFCG--MNATYEALTSKRPCGPSKKAIRGATYDLS 255
             G  YVHCTAG+GRAPAVA+ Y++W  G  +   ++ L SKR C P   AIR AT D+ 
Sbjct: 191 NGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDIL 250

Query: 256 KNDPWKEPFESLPDNAFEDI 265
                K    +L D  F  +
Sbjct: 251 TGLKRKTVTLTLKDKGFSRV 269

BLAST of Lag0041015.1 vs. TAIR 10
Match: AT3G52180.2 (dual specificity protein phosphatase (DsPTP1) family protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.1e-35
Identity = 96/260 (36.92%), Postives = 134/260 (51.54%), Query Frame = 0

Query: 16  AVSPLQNHA-----HNKSLPFCSKLSNPHFRSNKIWCGMPESGIRQNPTSGRAS---TSK 75
           +VSPL         H+ S      L +P  ++N     +    + ++ +S   S     +
Sbjct: 11  SVSPLLGFGCIQRDHSSSSSSLKMLISPPIKANDPKSRLVLHAVSESKSSSEMSGVAKDE 70

Query: 76  NRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFLKEEEGVAYIL 135
            + +EY+  M + M     Y HELGMNY  I  +LIVGS  Q PED+D L+ + GV  I 
Sbjct: 71  EKSDEYSQDMTQAMGAVLTYRHELGMNYNFIRPDLIVGSCLQTPEDVDKLR-KIGVKTIF 130

Query: 136 NLQQDKDVEYWGINLQSIIDRCKELG-IHHMRQPAKDFDPDSLRNGLPKAVSLLEWAISE 195
            LQQD D+EY+G+++ SI    K+   I H+R   +DFD   LR  LP  V  L  A+  
Sbjct: 131 CLQQDPDLEYFGVDISSIQAYAKKYSDIQHIRCEIRDFDAFDLRMRLPAVVGTLYKAVKR 190

Query: 196 GKGKVYVHCTAGLGRAPAVAIAYLYWFCG--MNATYEALTSKRPCGPSKKAIRGATYDLS 255
             G  YVHCTAG+GRAPAVA+ Y++W  G  +   ++ L SKR C P   AIR AT D+ 
Sbjct: 191 NGGVTYVHCTAGMGRAPAVALTYMFWVQGYKLMEAHKLLMSKRSCFPKLDAIRNATIDIL 250

Query: 256 KNDPWKEPFESLPDNAFEDI 265
                K    +L D  F  +
Sbjct: 251 TGLKRKTVTLTLKDKGFSRV 269

BLAST of Lag0041015.1 vs. TAIR 10
Match: AT3G01510.1 (like SEX4 1 )

HSP 1 Score: 112.8 bits (281), Expect = 4.4e-25
Identity = 68/188 (36.17%), Postives = 97/188 (51.60%), Query Frame = 0

Query: 58  PTSGRASTSKNRMEEYNLAMKKMMRNPYEYHHELGMNYTLIADNLIVGSQPQKPEDIDFL 117
           P  G        +EEY  A+ +  +    Y+H LGM Y+ I + + VGS  Q  ED++ L
Sbjct: 256 PGDGEWGYGNFPLEEYIKALDR-SKGELSYNHALGMRYSKITEQIYVGSCIQTEEDVENL 315

Query: 118 KEEEGVAYILNLQQDKDVEYWGINLQSIIDRCKELGIHHMRQPAKDFDPDSLRNGLPKAV 177
             E G+  ILN Q   + + WGI+ QSI D C++  +  +  P KD D   LR  LP  V
Sbjct: 316 -SEAGITAILNFQGGTEAQNWGIDSQSINDACQKSEVLMINYPIKDADSFDLRKKLPLCV 375

Query: 178 SLLEWAISEGKGKVYVHCTAGLGRAPAVAIAYLYWF--CGMNATYEALTSKRPCGPSKKA 237
            LL   + +   +V+V CT G  R+ A  IAYL+W     ++A Y  +T    C P + A
Sbjct: 376 GLLLRLLKKNH-RVFVTCTTGFDRSSACVIAYLHWMTDTSLHAAYSFVTGLHACKPDRPA 435

Query: 238 IRGATYDL 244
           I  AT+DL
Sbjct: 436 IAWATWDL 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022942267.12.4e-14289.01phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita moschata][more]
XP_022984858.13.1e-14289.01phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita maxima][more]
XP_023542341.11.2e-14188.65phosphoglucan phosphatase LSF2, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_011655031.14.0e-13785.11phosphoglucan phosphatase LSF2, chloroplastic [Cucumis sativus] >XP_011655032.1 ... [more]
XP_008456918.15.2e-13785.46PREDICTED: phosphoglucan phosphatase LSF2, chloroplastic isoform X3 [Cucumis mel... [more]
Match NameE-valueIdentityDescription
Q9SRK55.4e-10576.96Phosphoglucan phosphatase LSF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FEB52.9e-3436.92Phosphoglucan phosphatase DSP4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
G4LTX47.2e-3338.46Phosphoglucan phosphatase DSP4, amyloplastic OS=Castanea sativa OX=21020 GN=DSP4... [more]
F4J1176.1e-2436.17Phosphoglucan phosphatase LSF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q1M1993.0e-1033.11Laforin OS=Canis lupus familiaris OX=9615 GN=EPM2A PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FPR71.2e-14289.01phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JBS61.5e-14289.01phosphoglucan phosphatase LSF2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0KQM61.9e-13785.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218830 PE=4 SV=1[more]
A0A1S3C4E02.5e-13785.46phosphoglucan phosphatase LSF2, chloroplastic isoform X3 OS=Cucumis melo OX=3656... [more]
A0A1S3C3W88.9e-13585.20phosphoglucan phosphatase LSF2, chloroplastic isoform X2 OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT3G10940.13.9e-10676.96dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G52180.12.1e-3536.92dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G52180.22.1e-3536.92dual specificity protein phosphatase (DsPTP1) family protein [more]
AT3G01510.14.4e-2536.17like SEX4 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020422Dual specificity protein phosphatase domainSMARTSM00195dsp_5coord: 94..234
e-value: 0.0021
score: 7.9
IPR020422Dual specificity protein phosphatase domainPROSITEPS50054TYR_PHOSPHATASE_DUALcoord: 94..255
score: 23.061958
IPR000340Dual specificity phosphatase, catalytic domainPFAMPF00782DSPccoord: 118..228
e-value: 1.8E-12
score: 47.2
IPR029021Protein-tyrosine phosphatase-likeGENE3D3.90.190.10Protein tyrosine phosphatase superfamilycoord: 77..282
e-value: 3.8E-65
score: 221.2
IPR029021Protein-tyrosine phosphatase-likeSUPERFAMILY52799(Phosphotyrosine protein) phosphatases IIcoord: 86..228
NoneNo IPR availablePANTHERPTHR46642:SF2PHOSPHOGLUCAN PHOSPHATASE LSF2, CHLOROPLASTICcoord: 26..282
NoneNo IPR availablePANTHERPTHR46642DUAL SPECIFICITY PHOSPHATASE, SUBGROUP, CATALYTIC DOMAINcoord: 26..282
NoneNo IPR availableCDDcd14526DSP_laforin-likecoord: 93..238
e-value: 7.52323E-77
score: 228.62
IPR000387Tyrosine specific protein phosphatases domainPROSITEPS50056TYR_PHOSPHATASE_2coord: 171..210
score: 9.31388

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0041015Lag0041015gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0041015.1.exon4Lag0041015.1.exon4exon
Lag0041015.1.exon3Lag0041015.1.exon3exon
Lag0041015.1.exon2Lag0041015.1.exon2exon
Lag0041015.1.exon1Lag0041015.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0041015.1cds.Lag0041015.1_4CDS
cds.Lag0041015.1cds.Lag0041015.1_3CDS
cds.Lag0041015.1cds.Lag0041015.1_2CDS
cds.Lag0041015.1cds.Lag0041015.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0041015.1Lag0041015.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0046838 phosphorylated carbohydrate dephosphorylation
biological_process GO:0005983 starch catabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016791 phosphatase activity
molecular_function GO:0008138 protein tyrosine/serine/threonine phosphatase activity
molecular_function GO:0019203 carbohydrate phosphatase activity
molecular_function GO:2001070 starch binding