Lag0040642.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0040642.1 vs. NCBI nr
Match: XP_023513129.1 (putative GEM-like protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.0 bits (946), Expect = 2.9e-98
Identity = 190/224 (84.82%), Postives = 206/224 (91.96%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MK TIQE +L G QMKSL LARAR L DPSKHLH+SSSDDG QSIKKYNGKDWILNRV+K
Sbjct: 1   MKITIQEHVL-GAQMKSLGLARARFLADPSKHLHMSSSDDGYQSIKKYNGKDWILNRVHK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NGRK DNII ALREHVKLGAKI++TVKGKLSLGARI+RVGG+RKIYKKLF+V+EGEKLLK
Sbjct: 61  NGRKPDNIIRALREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNG-DYIRFHYKVVIPVEKVMRVN 180
           ASQCYLSTTAGP+AGLLFISTH IAFCSDKSIK SSPNG +++R HYKVVIP EKVMRVN
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKFSSPNGEEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQGLEGR 224
           ESENV+KASQKYIQI TVDNFEFWFMGFLNYQSTFNS+Q L+ R
Sbjct: 181 ESENVKKASQKYIQIVTVDNFEFWFMGFLNYQSTFNSLQELQKR 223

BLAST of Lag0040642.1 vs. NCBI nr
Match: XP_038892955.1 (GEM-like protein 7 [Benincasa hispida])

HSP 1 Score: 365.2 bits (936), Expect = 4.2e-97
Identity = 180/218 (82.57%), Postives = 201/218 (92.20%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MKTTIQEQ++ GIQMKSL LARARLL DPSKHLHISSSDDGSQSIKKYNGKDW+LNR+NK
Sbjct: 1   MKTTIQEQVILGIQMKSLGLARARLLGDPSKHLHISSSDDGSQSIKKYNGKDWVLNRLNK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NG+KTDNIIHALREHVKLGAKIS+TVKGKLSLGARILRVGGLRKIYKKLFS+ EGEKL+K
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGLRKIYKKLFSMTEGEKLIK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNE 180
           ASQCYLSTTAGPLAGLLFIS+HKIAFCSDKSIK+SS NGD+IR HYKV IP+ K+  V +
Sbjct: 121 ASQCYLSTTAGPLAGLLFISSHKIAFCSDKSIKLSSQNGDHIRIHYKVTIPLGKIKTVFQ 180

Query: 181 SENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQ 219
           SENV+  S+KY++I TVD+FEFWFMGFLNY  +FN +Q
Sbjct: 181 SENVKNPSEKYMEIVTVDDFEFWFMGFLNYHKSFNCIQ 218

BLAST of Lag0040642.1 vs. NCBI nr
Match: XP_022999500.1 (putative GEM-like protein 8 [Cucurbita maxima])

HSP 1 Score: 363.2 bits (931), Expect = 1.6e-96
Identity = 186/222 (83.78%), Postives = 203/222 (91.44%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MK TIQE  +FG QMKSL LARAR L DPSKHLH+SS DDG QSIKKYNGKDWILNRV+K
Sbjct: 1   MKITIQEH-VFGAQMKSLGLARARFLADPSKHLHMSSLDDGYQSIKKYNGKDWILNRVHK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NGRK DNII ALREHVKLGAKI++TVKGKLSLGARI+RVGG+RKIYKKLF+V+EGEKLLK
Sbjct: 61  NGRKPDNIIRALREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNG-DYIRFHYKVVIPVEKVMRVN 180
           ASQCYLSTT GP+AGLLFISTH IAFCSDKSIK+SSPNG +++R HYKVVIP EKVMRVN
Sbjct: 121 ASQCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQGLE 222
           ESENV+KASQKYIQI TVD FEFWFMGFLNYQSTFNS+Q L+
Sbjct: 181 ESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTFNSLQELQ 221

BLAST of Lag0040642.1 vs. NCBI nr
Match: XP_022956364.1 (GEM-like protein 4 [Cucurbita moschata])

HSP 1 Score: 360.5 bits (924), Expect = 1.0e-95
Identity = 184/222 (82.88%), Postives = 202/222 (90.99%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MK TIQE  +FG QMKSL LARAR L DPSKH H+SS DDGSQSI+KYNGKDWILNRV+K
Sbjct: 1   MKITIQEH-VFGAQMKSLGLARARFLADPSKHFHMSSLDDGSQSIQKYNGKDWILNRVHK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NGRK DNII ALREHVKLGAKI++TVKGKL LGARI+RVGG+RKIYKKLF+V+EGEKLLK
Sbjct: 61  NGRKPDNIIRALREHVKLGAKITETVKGKLRLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNG-DYIRFHYKVVIPVEKVMRVN 180
           ASQCYLSTTAGP+AGLLFISTH IAFCSDKSIK+SSPNG +++R HYKVVIP EKVMRVN
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQGLE 222
           ESENV+KASQKYIQI TVD FEFWFMGFL YQSTFNS+Q L+
Sbjct: 181 ESENVKKASQKYIQIVTVDKFEFWFMGFLKYQSTFNSLQALQ 221

BLAST of Lag0040642.1 vs. NCBI nr
Match: XP_011655500.1 (GEM-like protein 7 [Cucumis sativus] >KGN51561.1 hypothetical protein Csa_008634 [Cucumis sativus])

HSP 1 Score: 357.8 bits (917), Expect = 6.7e-95
Identity = 178/218 (81.65%), Postives = 200/218 (91.74%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MKTTIQEQ++ GIQMKSL LARARLL DPSK+LHISSSDDGSQSIKKYNGKD ILNR+NK
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NG+KTDNIIHALREHVKLGAKIS+TVKGKLSLGARILRVGG+RKIYKKLFS++E EKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNE 180
            SQCYLSTTAGPL GLLFISTHKIAFCSDKSIK++SPNGD+IR HYKV IP+ K+ RV +
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180

Query: 181 SENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQ 219
           SENV+  S+KY++I TVDN+EFWFMGFLNY  +FN +Q
Sbjct: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQ 218

BLAST of Lag0040642.1 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 8.6e-53
Identity = 113/203 (55.67%), Postives = 144/203 (70.94%), Query Frame = 0

Query: 26  LPDP---SKHLHISSSDDGSQSIKKYNGKDWILNRVNKNGRKTDNIIHALREHVKLGAKI 85
           LPDP   +K    +SS    QS  K      IL R     +KTD   + +R+  K+  K+
Sbjct: 24  LPDPASFNKFRVPASSKKSEQSNVKS-----ILKR-----KKTDGFTNGVRDQSKIRPKL 83

Query: 86  SDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGPLAGLLFISTH 145
           ++TVK KLSLGARIL+VGGL KI+K+LF V+EGEKL K  QCYLSTTAGP+AGLLFIS+ 
Sbjct: 84  TETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSK 143

Query: 146 KIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYIQIETVDNFEF 205
           K+AFCS++SIKV SP GD IR HYKV IP+ K+ RVN+S+N +K SQKY+++ TVD F+F
Sbjct: 144 KMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDF 203

Query: 206 WFMGFLNYQSTFNSVQGLEGRSF 226
           WFMGFL+YQ  FN ++     SF
Sbjct: 204 WFMGFLSYQKAFNCLEKALSLSF 216

BLAST of Lag0040642.1 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.8e-51
Identity = 107/213 (50.23%), Postives = 143/213 (67.14%), Query Frame = 0

Query: 13  IQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNKNGRKTDNIIHAL 72
           I   ++  + A  LPDP+    +        S     GK  +        +K D+  + +
Sbjct: 11  ITFPAVKTSPAGYLPDPASINKLQIPTSSKFSFLTGKGKSML------RKKKNDSFTNGV 70

Query: 73  REHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGP 132
           R+  KLG K+++TVK KLSLGARIL++GGL KIYK+LF V++ EKL KA QCYLSTTAGP
Sbjct: 71  RDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGP 130

Query: 133 LAGLLFISTHKIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYI 192
           +AGLLFIS+ KIAFCS++SIKV+SP G+  R HYKV IP+ K+  VN+S+N  K SQKY+
Sbjct: 131 IAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYL 190

Query: 193 QIETVDNFEFWFMGFLNYQSTFNSVQGLEGRSF 226
           ++ TVD F+FWFMGFL+YQ  FN ++     SF
Sbjct: 191 EVVTVDGFDFWFMGFLSYQKAFNCLEQALSLSF 217

BLAST of Lag0040642.1 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 3.0e-50
Identity = 103/193 (53.37%), Postives = 136/193 (70.47%), Query Frame = 0

Query: 26  LPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNKNGRKTDNIIHALREHVKLGAKISDT 85
           LPDP+   +         S     GK  +        +KTD+  +  R+  KLG K+++T
Sbjct: 24  LPDPASINNHQIPTSSKVSYLTGKGKSML------RKKKTDSFTNGARDQDKLGPKLTET 83

Query: 86  VKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGPLAGLLFISTHKIA 145
           VK KLSLGA+IL++GGL KIYK+LF V + EKL KA QCYLSTTAGP+AGLLFIS+ KIA
Sbjct: 84  VKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGLLFISSKKIA 143

Query: 146 FCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYIQIETVDNFEFWFM 205
           FCS++SIKV+SP G   R HYKV IP+ K+  VN+S+N +K SQKY++I T+DNF+FWFM
Sbjct: 144 FCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKKPSQKYLEIVTIDNFDFWFM 203

Query: 206 GFLNYQSTFNSVQ 219
           GF++YQ  FN ++
Sbjct: 204 GFVSYQKAFNCLE 210

BLAST of Lag0040642.1 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 2.6e-49
Identity = 93/156 (59.62%), Postives = 125/156 (80.13%), Query Frame = 0

Query: 63  RKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKAS 122
           +KTD+  +  R+  KLG K+++TVK KLSLGA+IL++GGL KIYK+LF V + EKL KA 
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 123 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESE 182
           QCYLSTT G +AGLLFIS+ KIAFCS++SIKV+SP GD  R HYKV IP+ K+  VN+S+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 183 NVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQ 219
           N +K SQ+Y+++ TVDN++FWFMGF++YQ  FN ++
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

BLAST of Lag0040642.1 vs. ExPASy Swiss-Prot
Match: Q9M122 (GEM-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 8.3e-32
Identity = 73/192 (38.02%), Postives = 118/192 (61.46%), Query Frame = 0

Query: 32  HLHISSSDDGSQSIKKYNGKDWILNRVNKNG-------RKTDNIIHALREHVKLGAKISD 91
           ++HI+S    S        KD +L  +N+ G       RK + ++  L++H+K    ISD
Sbjct: 31  YVHITSPTSASDK----RSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISD 90

Query: 92  TVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGPLAGLLFISTHKI 151
               +LS G +++  GG  +++++ F V   EKLL +  CY+STT+GP+ G+++IS  +I
Sbjct: 91  AAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRI 150

Query: 152 AFCSDKSIKV-SSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYIQIETVDNFEFW 211
           AFCSD +I++ SS  G+ +  +YKVV+  EK+  ++ S NV K S++Y+ + T D FEFW
Sbjct: 151 AFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFW 210

Query: 212 FMGFLNYQSTFN 216
           FMGF++Y   FN
Sbjct: 211 FMGFVSYIDAFN 218

BLAST of Lag0040642.1 vs. ExPASy TrEMBL
Match: A0A6J1KFK0 (putative GEM-like protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111493841 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.7e-97
Identity = 186/222 (83.78%), Postives = 203/222 (91.44%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MK TIQE  +FG QMKSL LARAR L DPSKHLH+SS DDG QSIKKYNGKDWILNRV+K
Sbjct: 1   MKITIQEH-VFGAQMKSLGLARARFLADPSKHLHMSSLDDGYQSIKKYNGKDWILNRVHK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NGRK DNII ALREHVKLGAKI++TVKGKLSLGARI+RVGG+RKIYKKLF+V+EGEKLLK
Sbjct: 61  NGRKPDNIIRALREHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNG-DYIRFHYKVVIPVEKVMRVN 180
           ASQCYLSTT GP+AGLLFISTH IAFCSDKSIK+SSPNG +++R HYKVVIP EKVMRVN
Sbjct: 121 ASQCYLSTTGGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQGLE 222
           ESENV+KASQKYIQI TVD FEFWFMGFLNYQSTFNS+Q L+
Sbjct: 181 ESENVKKASQKYIQIVTVDKFEFWFMGFLNYQSTFNSLQELQ 221

BLAST of Lag0040642.1 vs. ExPASy TrEMBL
Match: A0A6J1GYU0 (GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458125 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 5.0e-96
Identity = 184/222 (82.88%), Postives = 202/222 (90.99%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MK TIQE  +FG QMKSL LARAR L DPSKH H+SS DDGSQSI+KYNGKDWILNRV+K
Sbjct: 1   MKITIQEH-VFGAQMKSLGLARARFLADPSKHFHMSSLDDGSQSIQKYNGKDWILNRVHK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NGRK DNII ALREHVKLGAKI++TVKGKL LGARI+RVGG+RKIYKKLF+V+EGEKLLK
Sbjct: 61  NGRKPDNIIRALREHVKLGAKITETVKGKLRLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNG-DYIRFHYKVVIPVEKVMRVN 180
           ASQCYLSTTAGP+AGLLFISTH IAFCSDKSIK+SSPNG +++R HYKVVIP EKVMRVN
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQGLE 222
           ESENV+KASQKYIQI TVD FEFWFMGFL YQSTFNS+Q L+
Sbjct: 181 ESENVKKASQKYIQIVTVDKFEFWFMGFLKYQSTFNSLQALQ 221

BLAST of Lag0040642.1 vs. ExPASy TrEMBL
Match: A0A0A0KV22 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G578990 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.2e-95
Identity = 178/218 (81.65%), Postives = 200/218 (91.74%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MKTTIQEQ++ GIQMKSL LARARLL DPSK+LHISSSDDGSQSIKKYNGKD ILNR+NK
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQSIKKYNGKDCILNRLNK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NG+KTDNIIHALREHVKLGAKIS+TVKGKLSLGARILRVGG+RKIYKKLFS++E EKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNE 180
            SQCYLSTTAGPL GLLFISTHKIAFCSDKSIK++SPNGD+IR HYKV IP+ K+ RV +
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVFQ 180

Query: 181 SENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQ 219
           SENV+  S+KY++I TVDN+EFWFMGFLNY  +FN +Q
Sbjct: 181 SENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQ 218

BLAST of Lag0040642.1 vs. ExPASy TrEMBL
Match: A0A6J1GXL4 (GEM-like protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111458126 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.6e-94
Identity = 182/222 (81.98%), Postives = 204/222 (91.89%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MK+ IQ+Q+L G+Q+ SL LARAR L DPSKH H+SS DDGSQSI+KYNGKDWILNRV+K
Sbjct: 1   MKSAIQQQVL-GMQL-SLGLARARFLADPSKHFHMSSLDDGSQSIQKYNGKDWILNRVHK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NGRK DNII ALREHVKLGAKI++TVKGKL LGARI+RVGG+RKIYKKLF+V+EGEKLLK
Sbjct: 61  NGRKPDNIIRALREHVKLGAKITETVKGKLRLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNG-DYIRFHYKVVIPVEKVMRVN 180
           ASQCYLSTTAGP+AGLLFISTH IAFCSDKSIK+SSPNG +++R HYKVVIP EKVMRVN
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVVIPNEKVMRVN 180

Query: 181 ESENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQGLE 222
           ESENV+KASQKYIQI TVD FEFWFMGFL YQSTFNS+QGL+
Sbjct: 181 ESENVKKASQKYIQIVTVDKFEFWFMGFLKYQSTFNSLQGLQ 220

BLAST of Lag0040642.1 vs. ExPASy TrEMBL
Match: A0A6J1GW39 (putative GEM-like protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111458124 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.0e-93
Identity = 178/219 (81.28%), Postives = 201/219 (91.78%), Query Frame = 0

Query: 1   MKTTIQEQMLFGIQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNK 60
           MKTTIQEQ+L G QMKSLALARAR L DPSKHLHISSSDDGSQSIKKYNGKDWILNR++K
Sbjct: 1   MKTTIQEQVL-GAQMKSLALARARFLADPSKHLHISSSDDGSQSIKKYNGKDWILNRLHK 60

Query: 61  NGRKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLK 120
           NGRKTDNIIHALR+HVKLGAKI++TVKGKLSLGARI+RVGG+RKIYKKLF+V+EGEKLLK
Sbjct: 61  NGRKTDNIIHALRQHVKLGAKITETVKGKLSLGARIVRVGGVRKIYKKLFTVSEGEKLLK 120

Query: 121 ASQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNG-DYIRFHYKVVIPVEKVMRVN 180
           ASQCYLSTTAGP+AGLLFISTH IAFCSDKSIK+SSPNG +++R HYKV IP+ K+ RV 
Sbjct: 121 ASQCYLSTTAGPMAGLLFISTHNIAFCSDKSIKLSSPNGEEHVRIHYKVTIPLGKIRRVF 180

Query: 181 ESENVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQ 219
           ESENV+   +KYI++ TVD+FEFWFMGFLNY  TFN +Q
Sbjct: 181 ESENVKNPWEKYIEMVTVDDFEFWFMGFLNYHKTFNCLQ 218

BLAST of Lag0040642.1 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 208.4 bits (529), Expect = 6.1e-54
Identity = 113/203 (55.67%), Postives = 144/203 (70.94%), Query Frame = 0

Query: 26  LPDP---SKHLHISSSDDGSQSIKKYNGKDWILNRVNKNGRKTDNIIHALREHVKLGAKI 85
           LPDP   +K    +SS    QS  K      IL R     +KTD   + +R+  K+  K+
Sbjct: 24  LPDPASFNKFRVPASSKKSEQSNVKS-----ILKR-----KKTDGFTNGVRDQSKIRPKL 83

Query: 86  SDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGPLAGLLFISTH 145
           ++TVK KLSLGARIL+VGGL KI+K+LF V+EGEKL K  QCYLSTTAGP+AGLLFIS+ 
Sbjct: 84  TETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSK 143

Query: 146 KIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYIQIETVDNFEF 205
           K+AFCS++SIKV SP GD IR HYKV IP+ K+ RVN+S+N +K SQKY+++ TVD F+F
Sbjct: 144 KMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDF 203

Query: 206 WFMGFLNYQSTFNSVQGLEGRSF 226
           WFMGFL+YQ  FN ++     SF
Sbjct: 204 WFMGFLSYQKAFNCLEKALSLSF 216

BLAST of Lag0040642.1 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 203.4 bits (516), Expect = 2.0e-52
Identity = 107/213 (50.23%), Postives = 143/213 (67.14%), Query Frame = 0

Query: 13  IQMKSLALARARLLPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNKNGRKTDNIIHAL 72
           I   ++  + A  LPDP+    +        S     GK  +        +K D+  + +
Sbjct: 11  ITFPAVKTSPAGYLPDPASINKLQIPTSSKFSFLTGKGKSML------RKKKNDSFTNGV 70

Query: 73  REHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGP 132
           R+  KLG K+++TVK KLSLGARIL++GGL KIYK+LF V++ EKL KA QCYLSTTAGP
Sbjct: 71  RDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGP 130

Query: 133 LAGLLFISTHKIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYI 192
           +AGLLFIS+ KIAFCS++SIKV+SP G+  R HYKV IP+ K+  VN+S+N  K SQKY+
Sbjct: 131 IAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQNTTKPSQKYL 190

Query: 193 QIETVDNFEFWFMGFLNYQSTFNSVQGLEGRSF 226
           ++ TVD F+FWFMGFL+YQ  FN ++     SF
Sbjct: 191 EVVTVDGFDFWFMGFLSYQKAFNCLEQALSLSF 217

BLAST of Lag0040642.1 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 199.9 bits (507), Expect = 2.2e-51
Identity = 103/193 (53.37%), Postives = 136/193 (70.47%), Query Frame = 0

Query: 26  LPDPSKHLHISSSDDGSQSIKKYNGKDWILNRVNKNGRKTDNIIHALREHVKLGAKISDT 85
           LPDP+   +         S     GK  +        +KTD+  +  R+  KLG K+++T
Sbjct: 86  LPDPASINNHQIPTSSKVSYLTGKGKSML------RKKKTDSFTNGARDQDKLGPKLTET 145

Query: 86  VKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGPLAGLLFISTHKIA 145
           VK KLSLGA+IL++GGL KIYK+LF V + EKL KA QCYLSTTAGP+AGLLFIS+ KIA
Sbjct: 146 VKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGLLFISSKKIA 205

Query: 146 FCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYIQIETVDNFEFWFM 205
           FCS++SIKV+SP G   R HYKV IP+ K+  VN+S+N +K SQKY++I T+DNF+FWFM
Sbjct: 206 FCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQNTKKPSQKYLEIVTIDNFDFWFM 265

Query: 206 GFLNYQSTFNSVQ 219
           GF++YQ  FN ++
Sbjct: 266 GFVSYQKAFNCLE 272

BLAST of Lag0040642.1 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 196.8 bits (499), Expect = 1.8e-50
Identity = 93/156 (59.62%), Postives = 125/156 (80.13%), Query Frame = 0

Query: 63  RKTDNIIHALREHVKLGAKISDTVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKAS 122
           +KTD+  +  R+  KLG K+++TVK KLSLGA+IL++GGL KIYK+LF V + EKL KA 
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 123 QCYLSTTAGPLAGLLFISTHKIAFCSDKSIKVSSPNGDYIRFHYKVVIPVEKVMRVNESE 182
           QCYLSTT G +AGLLFIS+ KIAFCS++SIKV+SP GD  R HYKV IP+ K+  VN+S+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 183 NVEKASQKYIQIETVDNFEFWFMGFLNYQSTFNSVQ 219
           N +K SQ+Y+++ TVDN++FWFMGF++YQ  FN ++
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

BLAST of Lag0040642.1 vs. TAIR 10
Match: AT4G01600.1 (GRAM domain family protein )

HSP 1 Score: 138.7 bits (348), Expect = 5.9e-33
Identity = 73/192 (38.02%), Postives = 118/192 (61.46%), Query Frame = 0

Query: 32  HLHISSSDDGSQSIKKYNGKDWILNRVNKNG-------RKTDNIIHALREHVKLGAKISD 91
           ++HI+S    S        KD +L  +N+ G       RK + ++  L++H+K    ISD
Sbjct: 31  YVHITSPTSASDK----RSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISD 90

Query: 92  TVKGKLSLGARILRVGGLRKIYKKLFSVNEGEKLLKASQCYLSTTAGPLAGLLFISTHKI 151
               +LS G +++  GG  +++++ F V   EKLL +  CY+STT+GP+ G+++IS  +I
Sbjct: 91  AAMARLSQGTKMIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRI 150

Query: 152 AFCSDKSIKV-SSPNGDYIRFHYKVVIPVEKVMRVNESENVEKASQKYIQIETVDNFEFW 211
           AFCSD +I++ SS  G+ +  +YKVV+  EK+  ++ S NV K S++Y+ + T D FEFW
Sbjct: 151 AFCSDYAIRLPSSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFW 210

Query: 212 FMGFLNYQSTFN 216
           FMGF++Y   FN
Sbjct: 211 FMGFVSYIDAFN 218