Lag0040627.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0040627.1 vs. NCBI nr
Match: XP_008389053.1 (alpha-galactosidase 1 isoform X1 [Malus domestica])

HSP 1 Score: 644.4 bits (1661), Expect = 6.3e-181
Identity = 292/395 (73.92%), Postives = 340/395 (86.08%), Query Frame = 0

Query: 6   IASILISCLLIAPSSSATSRKIDPENRRSLLANGLGLTPPMGWNSWNHFRCGINETIVKQ 65
           I  +LI  ++++ +SSA++     + RR+LLANGLGLTPPMGWNSWNHF C I+E I+K 
Sbjct: 21  IVMLLIMAMVVSFASSASAAAASSQLRRNLLANGLGLTPPMGWNSWNHFNCKIDEKIIKA 80

Query: 66  NADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSDYVHSKGLKL 125
            AD L+STGLSKLGY YVNIDDCWAE+ RD    LVPKK+TFPSGIKAL+DYVHSKGLKL
Sbjct: 81  TADALVSTGLSKLGYTYVNIDDCWAEIARDHKRNLVPKKSTFPSGIKALADYVHSKGLKL 140

Query: 126 GIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTERYPIMTRAL 185
           GIYSDAG FTCS+TMPGSL HEEQDAKTFA WGIDYLKYDNCNND+ KPT RYP+MTRAL
Sbjct: 141 GIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDNCNNDDSKPTVRYPVMTRAL 200

Query: 186 MKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLNDAFAEHARPG 245
           MKAGRPIF SLCEWGD+HPA W A VGNSWRTT+DI D W+SM+SRAD+N+ +AE ARPG
Sbjct: 201 MKAGRPIFFSLCEWGDLHPAQWGAKVGNSWRTTNDISDTWESMISRADMNEVYAEFARPG 260

Query: 246 GWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEIIGNSEVIAVN 305
           GWNDPDMLEVGNGGMTK+EY VHFSIWAISKAPLLLG D+R I+ + MEII N EVI+VN
Sbjct: 261 GWNDPDMLEVGNGGMTKDEYVVHFSIWAISKAPLLLGCDVRNITKENMEIIANKEVISVN 320

Query: 306 QDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQA 365
           QDPLGVQ +KVRSEGD+E+WAGPLSGYRVA+LLVNR  WR A  AHWDDI IP +SVV+A
Sbjct: 321 QDPLGVQAKKVRSEGDLEIWAGPLSGYRVALLLVNRGPWRTAVTAHWDDIEIPTNSVVEA 380

Query: 366 RDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
           RDLW+HKTL+ +FVGN++  +++H+CKMY+L P+S
Sbjct: 381 RDLWEHKTLKARFVGNLTATVDSHACKMYVLKPVS 415

BLAST of Lag0040627.1 vs. NCBI nr
Match: TQD99858.1 (hypothetical protein C1H46_014596 [Malus baccata])

HSP 1 Score: 636.3 bits (1640), Expect = 1.7e-178
Identity = 290/382 (75.92%), Postives = 331/382 (86.65%), Query Frame = 0

Query: 19  SSSATSRKIDPENRRSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKL 78
           +SSA S     + RR+LLANGLGLTPPMGWNSWNHF C I+E I+K  AD L+STGLSKL
Sbjct: 550 TSSAAS--ASSQLRRNLLANGLGLTPPMGWNSWNHFNCKIDEKIIKATADALVSTGLSKL 609

Query: 79  GYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSR 138
           GY YVNIDDCWAE+ RD    LVPKK+TFPSGIKAL+DYVHSKGLKLGIYSDAG FTCS+
Sbjct: 610 GYTYVNIDDCWAEIARDHKRNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDAGYFTCSK 669

Query: 139 TMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCE 198
           TMPGSL HEEQDAKTFA WGIDYLKYDNCNND+ KPT RYP+MTRALMKAGRPIF SLCE
Sbjct: 670 TMPGSLGHEEQDAKTFAAWGIDYLKYDNCNNDDSKPTVRYPVMTRALMKAGRPIFFSLCE 729

Query: 199 WGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNG 258
           WGD+HPA W A VGNSWRTT+DI D W+SM+SRAD+N+ +AE ARPGGWNDPDMLEVGNG
Sbjct: 730 WGDLHPAQWGAKVGNSWRTTNDISDTWESMISRADMNEVYAEFARPGGWNDPDMLEVGNG 789

Query: 259 GMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRS 318
           GMTK+EY VHFSIWAISKAPLLLG D+R I+ + MEII N EVI+VNQDPLGVQ +KVRS
Sbjct: 790 GMTKDEYVVHFSIWAISKAPLLLGCDVRNITKENMEIIANKEVISVNQDPLGVQAKKVRS 849

Query: 319 EGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKTLEKQF 378
           EGD+E+WAGPLSGYRVA+LLVNR  WR A  AHWDDI IP +SVV+ARDLW+HKTL+ +F
Sbjct: 850 EGDLEIWAGPLSGYRVALLLVNRGPWRTAVTAHWDDIEIPTNSVVKARDLWEHKTLKARF 909

Query: 379 VGNISNILEAHSCKMYILTPIS 401
           VGN++  +++H+CKMY+L P+S
Sbjct: 910 VGNLTATVDSHACKMYVLKPVS 929

BLAST of Lag0040627.1 vs. NCBI nr
Match: XP_024193758.1 (alpha-galactosidase 1 [Rosa chinensis] >PRQ41470.1 putative alpha-galactosidase [Rosa chinensis])

HSP 1 Score: 635.6 bits (1638), Expect = 2.9e-178
Identity = 290/409 (70.90%), Postives = 343/409 (83.86%), Query Frame = 0

Query: 3   KLTIASILISCLLIAPS-------SSATSRKI----DPENRRSLLANGLGLTPPMGWNSW 62
           ++ + +++I C  I+P        +S  +R +      + RR+LLANGLG+TPPMGWNSW
Sbjct: 17  RMIVMTVMIVCSFISPQPMLMGVCASPITRMMVADTKEQQRRNLLANGLGVTPPMGWNSW 76

Query: 63  NHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGI 122
           NHF C I+E I+K  AD LISTGLSKLGY YVNIDDCWAE+ RD  G LVPKK+TFPSGI
Sbjct: 77  NHFNCKIDEKIIKATADALISTGLSKLGYTYVNIDDCWAEVARDDKGNLVPKKSTFPSGI 136

Query: 123 KALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDN 182
           KAL+DYVHSKGLKLGIYSDAG FTCS+TMPGSL HEEQDAK FA WGIDYLKYDNCN D 
Sbjct: 137 KALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKIFAAWGIDYLKYDNCNTDG 196

Query: 183 IKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSR 242
            KPT RYP+MTRALMKA RPIF SLCEWGD+HPALW A VGNSWRTT+DI D W+SM+SR
Sbjct: 197 SKPTVRYPVMTRALMKADRPIFFSLCEWGDLHPALWGAKVGNSWRTTNDISDTWESMVSR 256

Query: 243 ADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDD 302
           AD+N+ +AE+ARPGGWNDPDMLEVGNGGMTK+EY VHFSIWAISKAPLL+G D+R ++ +
Sbjct: 257 ADMNEVYAEYARPGGWNDPDMLEVGNGGMTKDEYVVHFSIWAISKAPLLIGCDVRNMTKE 316

Query: 303 TMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAH 362
           TMEII N EVI+VNQDPLGVQ +KVR EGD+EVWAGPLSGYRVA+LLVNR  WR +  AH
Sbjct: 317 TMEIIANKEVISVNQDPLGVQAKKVRMEGDLEVWAGPLSGYRVALLLVNRGPWRTSITAH 376

Query: 363 WDDIGIPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
           W+DIGIP +SVV+ARDLW+HKTL+K+FVGN++  +++H+CK+Y+L PIS
Sbjct: 377 WEDIGIPPNSVVEARDLWEHKTLKKRFVGNLTATMDSHACKLYVLKPIS 425

BLAST of Lag0040627.1 vs. NCBI nr
Match: ARV78455.1 (alpha-galactosidase [Vernicia fordii])

HSP 1 Score: 634.8 bits (1636), Expect = 5.0e-178
Identity = 290/404 (71.78%), Postives = 337/404 (83.42%), Query Frame = 0

Query: 1   MEKLTIASILISCLLIAPSSSATSRKIDPEN----RRSLLANGLGLTPPMGWNSWNHFRC 60
           ME+ ++  + +  L++    S++S+          RR+LL NGLG+TPPMGWNSWNHF C
Sbjct: 1   MERCSVLGVWLLLLILMVEGSSSSKVTGSGKYNSLRRNLLTNGLGMTPPMGWNSWNHFWC 60

Query: 61  GINETIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSD 120
           GINE I+K +AD L+STGL KLGY YVNIDDCWAE+ RD  G LVPK +TFPSGIKAL+D
Sbjct: 61  GINENIIKDSADALVSTGLHKLGYTYVNIDDCWAEIARDEKGNLVPKNSTFPSGIKALAD 120

Query: 121 YVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTE 180
           YVHSKGLKLGIYSDAG FTCS TMPGSL HEEQDAKTFA WGIDYLKYDNCN D  KPT 
Sbjct: 121 YVHSKGLKLGIYSDAGYFTCSGTMPGSLGHEEQDAKTFASWGIDYLKYDNCNEDGTKPTI 180

Query: 181 RYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLND 240
           RYP+MTRALMKAGRPIF SLCEWGDMHPALW   VGNSWRTTSDI DNW+SM+SRAD+N+
Sbjct: 181 RYPVMTRALMKAGRPIFFSLCEWGDMHPALWGGKVGNSWRTTSDIYDNWESMVSRADINE 240

Query: 241 AFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEII 300
            +AE ARPGGWNDPDMLEVGNGGMTK+EY  HFSIWAISKAPLLLG D+R +S+DTMEI+
Sbjct: 241 VYAEFARPGGWNDPDMLEVGNGGMTKDEYIAHFSIWAISKAPLLLGCDVRNMSEDTMEIV 300

Query: 301 GNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIG 360
            N EVIA+NQDPLGVQ +KVR +GD+EVWAGPL+GYRVAVLL NR   R A  A+WDDIG
Sbjct: 301 ANKEVIAINQDPLGVQAKKVRMKGDLEVWAGPLTGYRVAVLLFNRGPSRSAISAYWDDIG 360

Query: 361 IPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
           IP +S+VQARD+W+HKTL ++FVGN++  +++H+CKMYIL PIS
Sbjct: 361 IPANSLVQARDVWEHKTLNREFVGNLTATVDSHACKMYILKPIS 404

BLAST of Lag0040627.1 vs. NCBI nr
Match: XP_010031355.2 (alpha-galactosidase 1 [Eucalyptus grandis])

HSP 1 Score: 634.0 bits (1634), Expect = 8.6e-178
Identity = 289/393 (73.54%), Postives = 326/393 (82.95%), Query Frame = 0

Query: 17  APSSSATSRKID----------PENRRSLLANGLGLTPPMGWNSWNHFRCGINETIVKQN 76
           AP  +A+  K+D           E RR+LLANGLGLTPPMGWNSWNHF C INET++++ 
Sbjct: 28  APGGAASKAKVDLKSSTYLHYKQEYRRNLLANGLGLTPPMGWNSWNHFACVINETVIRET 87

Query: 77  ADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLG 136
           AD L+STGLSKLGY YVNIDDCWAE+ RD  G  V K   FPSGIKAL+DYVHSKGLK+G
Sbjct: 88  ADALVSTGLSKLGYEYVNIDDCWAEIARDHHGNFVAKNTAFPSGIKALADYVHSKGLKIG 147

Query: 137 IYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALM 196
           IYSD+G FTCS+TMPGSL HEEQDAKTFA WGIDYLKYDNCNND  KPT RYP+MTRALM
Sbjct: 148 IYSDSGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDNCNNDGTKPTVRYPVMTRALM 207

Query: 197 KAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGG 256
           KAGRPIF SLCEWGD+HPALW A +GNSWRTT+DI D WDSMMSRADLN+ +AE ARPGG
Sbjct: 208 KAGRPIFFSLCEWGDLHPALWGAKIGNSWRTTNDISDTWDSMMSRADLNEPYAELARPGG 267

Query: 257 WNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQ 316
           WNDPDMLEVGNGGMTK EY VHFSIWAISKAPLLLG D+R +S DT EII N EVIAVNQ
Sbjct: 268 WNDPDMLEVGNGGMTKNEYMVHFSIWAISKAPLLLGCDIRNLSKDTWEIISNKEVIAVNQ 327

Query: 317 DPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQAR 376
           DPLG+Q +KVRSEGD EVWAGP++GYRVAVLLVNR  +R A  A WDDIG+P ++V +AR
Sbjct: 328 DPLGIQAKKVRSEGDQEVWAGPIAGYRVAVLLVNRGTYRYALTARWDDIGLPLETVAEAR 387

Query: 377 DLWQHKTLEKQFVGNISNILEAHSCKMYILTPI 400
           DLW+HKTL+  FVGNIS  ++ HSCK+YIL P+
Sbjct: 388 DLWEHKTLKNTFVGNISATIDGHSCKLYILKPV 420

BLAST of Lag0040627.1 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 1.0e-173
Identity = 280/402 (69.65%), Postives = 338/402 (84.08%), Query Frame = 0

Query: 3   KLTIASILISCL---LIAPSSSATSRKIDPE-NRRSLLANGLGLTPPMGWNSWNHFRCGI 62
           K+ I  ILIS +   ++  S S  +   D E  RR LL NGLG+TPPMGWNSWNHF C I
Sbjct: 9   KMPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNI 68

Query: 63  NETIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSDYV 122
           +E ++K+ AD L++TGLSKLGY YVNIDDCWAE+ RD  G LVPKK+TFPSGIKA++DYV
Sbjct: 69  DEKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYV 128

Query: 123 HSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTERY 182
           HSKGLKLGIYSDAG FTCS+TMPGSL +EE DAKTFAEWGIDYLKYDNCN+D  KPT RY
Sbjct: 129 HSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRY 188

Query: 183 PIMTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLNDAF 242
           P+MTRALMK+GRPIF SLCEWGDMHPALW + VGNSWRTT+DIKD W SM+S AD+N+ +
Sbjct: 189 PVMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVY 248

Query: 243 AEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEIIGN 302
           AEHARPGGWNDPDMLEVGNGGMTK+EY VHFSIWAISKAPLLLG D+R ++ +TMEI+ N
Sbjct: 249 AEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVAN 308

Query: 303 SEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIGIP 362
            EVIA+NQDP GVQ +KVR EGD+EVWAGPLSGYRVA+LL+NR   R +  A W+DI IP
Sbjct: 309 KEVIAINQDPHGVQAKKVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIP 368

Query: 363 KDSVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
            +S+V+ARDLW+H+TL+++FVGN++  +++H+CK+Y+L P++
Sbjct: 369 ANSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLYVLKPVA 410

BLAST of Lag0040627.1 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 2.7e-158
Identity = 251/367 (68.39%), Postives = 297/367 (80.93%), Query Frame = 0

Query: 32  RRSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAE 91
           RRSLLANGLGLTPPMGWNSWNHFRC ++E ++++ AD ++S GL+ LGY Y+N+DDCWAE
Sbjct: 12  RRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWAE 71

Query: 92  LIRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDA 151
           L RD  G LVPK +TFPSGIKAL+DYVHSKGLKLGIYSDAGT TCS+TMPGSL HEEQDA
Sbjct: 72  LNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQDA 131

Query: 152 KTFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADV 211
           KTFA WG+DYLKYDNCNN+NI P ERYPIM++AL+ +GR IF SLCEWG+  PA W+ +V
Sbjct: 132 KTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKEV 191

Query: 212 GNSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 271
           GNSWRTT DI D+W SM SRAD+ND +A +A PGGWNDPDMLEVGNGGMT  EYR HFSI
Sbjct: 192 GNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFSI 251

Query: 272 WAISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSG 331
           WA++KAPLL+G D+R +   T +++ N+EVIAVNQD LGVQG KV++ GD+EVWAGPLSG
Sbjct: 252 WALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLSG 311

Query: 332 YRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSC 391
            RVAV L NR        A+W D+G+P  +VV ARDLW H T EK   G IS  ++AH  
Sbjct: 312 KRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHST-EKSVKGQISAAVDAHDS 371

Query: 392 KMYILTP 399
           KMY+LTP
Sbjct: 372 KMYVLTP 377

BLAST of Lag0040627.1 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 7.9e-150
Identity = 240/369 (65.04%), Postives = 288/369 (78.05%), Query Frame = 0

Query: 32  RRSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAE 91
           RR L  NGLG TPPMGWNSWNHF C INE +V++ AD ++STGL+ LGY Y+N+DDCWAE
Sbjct: 44  RRYLAENGLGQTPPMGWNSWNHFGCDINENVVRETADAMVSTGLAALGYQYINLDDCWAE 103

Query: 92  LIRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDA 151
           L RD +G +VP  A FPSGIKAL+DYVHSKGLKLG+YSDAG  TCS+ MPGSL HEEQDA
Sbjct: 104 LNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVYSDAGNQTCSKRMPGSLGHEEQDA 163

Query: 152 KTFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADV 211
           KTFA WG+DYLKYDNC N  I   ERYP M +AL+ +GRPIF S+CEWG   P +W+  +
Sbjct: 164 KTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIFFSMCEWGWEDPQIWAKSI 223

Query: 212 GNSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 271
           GNSWRTT DI+DNW+SM S AD ND +A +A PGGWNDPDMLEVGNGGMT EEYR HFSI
Sbjct: 224 GNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDMLEVGNGGMTTEEYRSHFSI 283

Query: 272 WAISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSG 331
           WA++KAPLL+G D+R + D T E+I N+EVIAVNQD LGVQG+KV+S  D+EVWAGPLS 
Sbjct: 284 WALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSD 343

Query: 332 YRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSC 391
            +VAV+L NR+  R    A W DIG+ + + V ARDLW+H T +    G IS  +++H+C
Sbjct: 344 NKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDLWEHST-QSLVSGEISAEIDSHAC 403

Query: 392 KMYILTPIS 401
           KMY+LTP S
Sbjct: 404 KMYVLTPRS 411

BLAST of Lag0040627.1 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 8.7e-149
Identity = 242/368 (65.76%), Postives = 284/368 (77.17%), Query Frame = 0

Query: 33  RSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAEL 92
           R L+ NGL L+P MGWNSWNHF+C INET++KQ AD ++S+GLS +GY Y+NIDDCW EL
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 93  IRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAK 152
            RD  G LV K +TFPSGIKALSDYVHSKGLKLGIYSDAGT TCS+TMPGSL HEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 153 TFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVG 212
           TFA WGIDYLKYDNC N    P ERYP M++AL+ +GR IF SLCEWG   PA W+ D+G
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 213 NSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 272
           NSWRTT DI+DNW SM   AD ND +A +ARPG WNDPDMLEVGNGGMTKEEY  HFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 273 AISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGY 332
           A++KAPLL+G DLR +   T E++ N EVIAVNQD LG+QG+KV+ EGD+EVWAGPLS  
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

Query: 333 RVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKT---LEKQFVGNISNILEAH 392
           RVAV+L NR        A W +IG+    +V ARDLW+H T   ++KQ    +S ++E H
Sbjct: 329 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQ----LSALVEPH 388

Query: 393 SCKMYILT 398
           +CKMY LT
Sbjct: 389 ACKMYTLT 392

BLAST of Lag0040627.1 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.6e-142
Identity = 233/367 (63.49%), Postives = 273/367 (74.39%), Query Frame = 0

Query: 32  RRSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAE 91
           RR    NGLG TP MGWNSWNHF CGINE I+++ AD L++TGL+KLGY YVNIDDCWAE
Sbjct: 52  RRRAFENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAE 111

Query: 92  LIRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDA 151
             RD  G  VP + TFPSGIKAL+DYVH+KGLKLGIYSDAG+ TCS  MPGSL HEEQD 
Sbjct: 112 YSRDSQGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDV 171

Query: 152 KTFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADV 211
           KTFA WG+DYLKYDNCN+      ERY  M+ A+   G+ IF SLCEWG  +PA W+  +
Sbjct: 172 KTFASWGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRM 231

Query: 212 GNSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSI 271
           GNSWRTT DI DNW SM SRAD ND +A +A PGGWNDPDMLEVGNGGM++ EYR HFSI
Sbjct: 232 GNSWRTTGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSI 291

Query: 272 WAISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSG 331
           WA++KAPLL+G D+R +S  T  I+ NSEVIAVNQD LGVQG+KV+S+  +EVWAGPLS 
Sbjct: 292 WALAKAPLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSN 351

Query: 332 YRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSC 391
            R AV+L NR  ++    AHW +IG+     V ARDLW H +   Q  G IS  +  H C
Sbjct: 352 NRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFAAQ--GQISASVAPHDC 411

Query: 392 KMYILTP 399
           KMY+LTP
Sbjct: 412 KMYVLTP 416

BLAST of Lag0040627.1 vs. ExPASy TrEMBL
Match: A0A540MM48 (Pyruvate kinase OS=Malus baccata OX=106549 GN=C1H46_014596 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 8.4e-179
Identity = 290/382 (75.92%), Postives = 331/382 (86.65%), Query Frame = 0

Query: 19  SSSATSRKIDPENRRSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKL 78
           +SSA S     + RR+LLANGLGLTPPMGWNSWNHF C I+E I+K  AD L+STGLSKL
Sbjct: 550 TSSAAS--ASSQLRRNLLANGLGLTPPMGWNSWNHFNCKIDEKIIKATADALVSTGLSKL 609

Query: 79  GYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSR 138
           GY YVNIDDCWAE+ RD    LVPKK+TFPSGIKAL+DYVHSKGLKLGIYSDAG FTCS+
Sbjct: 610 GYTYVNIDDCWAEIARDHKRNLVPKKSTFPSGIKALADYVHSKGLKLGIYSDAGYFTCSK 669

Query: 139 TMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCE 198
           TMPGSL HEEQDAKTFA WGIDYLKYDNCNND+ KPT RYP+MTRALMKAGRPIF SLCE
Sbjct: 670 TMPGSLGHEEQDAKTFAAWGIDYLKYDNCNNDDSKPTVRYPVMTRALMKAGRPIFFSLCE 729

Query: 199 WGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNG 258
           WGD+HPA W A VGNSWRTT+DI D W+SM+SRAD+N+ +AE ARPGGWNDPDMLEVGNG
Sbjct: 730 WGDLHPAQWGAKVGNSWRTTNDISDTWESMISRADMNEVYAEFARPGGWNDPDMLEVGNG 789

Query: 259 GMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRS 318
           GMTK+EY VHFSIWAISKAPLLLG D+R I+ + MEII N EVI+VNQDPLGVQ +KVRS
Sbjct: 790 GMTKDEYVVHFSIWAISKAPLLLGCDVRNITKENMEIIANKEVISVNQDPLGVQAKKVRS 849

Query: 319 EGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKTLEKQF 378
           EGD+E+WAGPLSGYRVA+LLVNR  WR A  AHWDDI IP +SVV+ARDLW+HKTL+ +F
Sbjct: 850 EGDLEIWAGPLSGYRVALLLVNRGPWRTAVTAHWDDIEIPTNSVVKARDLWEHKTLKARF 909

Query: 379 VGNISNILEAHSCKMYILTPIS 401
           VGN++  +++H+CKMY+L P+S
Sbjct: 910 VGNLTATVDSHACKMYVLKPVS 929

BLAST of Lag0040627.1 vs. ExPASy TrEMBL
Match: A0A2P6R4V3 (Alpha-galactosidase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0447241 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.4e-178
Identity = 290/409 (70.90%), Postives = 343/409 (83.86%), Query Frame = 0

Query: 3   KLTIASILISCLLIAPS-------SSATSRKI----DPENRRSLLANGLGLTPPMGWNSW 62
           ++ + +++I C  I+P        +S  +R +      + RR+LLANGLG+TPPMGWNSW
Sbjct: 17  RMIVMTVMIVCSFISPQPMLMGVCASPITRMMVADTKEQQRRNLLANGLGVTPPMGWNSW 76

Query: 63  NHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGI 122
           NHF C I+E I+K  AD LISTGLSKLGY YVNIDDCWAE+ RD  G LVPKK+TFPSGI
Sbjct: 77  NHFNCKIDEKIIKATADALISTGLSKLGYTYVNIDDCWAEVARDDKGNLVPKKSTFPSGI 136

Query: 123 KALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDN 182
           KAL+DYVHSKGLKLGIYSDAG FTCS+TMPGSL HEEQDAK FA WGIDYLKYDNCN D 
Sbjct: 137 KALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKIFAAWGIDYLKYDNCNTDG 196

Query: 183 IKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSR 242
            KPT RYP+MTRALMKA RPIF SLCEWGD+HPALW A VGNSWRTT+DI D W+SM+SR
Sbjct: 197 SKPTVRYPVMTRALMKADRPIFFSLCEWGDLHPALWGAKVGNSWRTTNDISDTWESMVSR 256

Query: 243 ADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDD 302
           AD+N+ +AE+ARPGGWNDPDMLEVGNGGMTK+EY VHFSIWAISKAPLL+G D+R ++ +
Sbjct: 257 ADMNEVYAEYARPGGWNDPDMLEVGNGGMTKDEYVVHFSIWAISKAPLLIGCDVRNMTKE 316

Query: 303 TMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAH 362
           TMEII N EVI+VNQDPLGVQ +KVR EGD+EVWAGPLSGYRVA+LLVNR  WR +  AH
Sbjct: 317 TMEIIANKEVISVNQDPLGVQAKKVRMEGDLEVWAGPLSGYRVALLLVNRGPWRTSITAH 376

Query: 363 WDDIGIPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
           W+DIGIP +SVV+ARDLW+HKTL+K+FVGN++  +++H+CK+Y+L PIS
Sbjct: 377 WEDIGIPPNSVVEARDLWEHKTLKKRFVGNLTATMDSHACKLYVLKPIS 425

BLAST of Lag0040627.1 vs. ExPASy TrEMBL
Match: A0A1Z1G633 (Alpha-galactosidase OS=Vernicia fordii OX=73154 PE=2 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.4e-178
Identity = 290/404 (71.78%), Postives = 337/404 (83.42%), Query Frame = 0

Query: 1   MEKLTIASILISCLLIAPSSSATSRKIDPEN----RRSLLANGLGLTPPMGWNSWNHFRC 60
           ME+ ++  + +  L++    S++S+          RR+LL NGLG+TPPMGWNSWNHF C
Sbjct: 1   MERCSVLGVWLLLLILMVEGSSSSKVTGSGKYNSLRRNLLTNGLGMTPPMGWNSWNHFWC 60

Query: 61  GINETIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSD 120
           GINE I+K +AD L+STGL KLGY YVNIDDCWAE+ RD  G LVPK +TFPSGIKAL+D
Sbjct: 61  GINENIIKDSADALVSTGLHKLGYTYVNIDDCWAEIARDEKGNLVPKNSTFPSGIKALAD 120

Query: 121 YVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTE 180
           YVHSKGLKLGIYSDAG FTCS TMPGSL HEEQDAKTFA WGIDYLKYDNCN D  KPT 
Sbjct: 121 YVHSKGLKLGIYSDAGYFTCSGTMPGSLGHEEQDAKTFASWGIDYLKYDNCNEDGTKPTI 180

Query: 181 RYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLND 240
           RYP+MTRALMKAGRPIF SLCEWGDMHPALW   VGNSWRTTSDI DNW+SM+SRAD+N+
Sbjct: 181 RYPVMTRALMKAGRPIFFSLCEWGDMHPALWGGKVGNSWRTTSDIYDNWESMVSRADINE 240

Query: 241 AFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEII 300
            +AE ARPGGWNDPDMLEVGNGGMTK+EY  HFSIWAISKAPLLLG D+R +S+DTMEI+
Sbjct: 241 VYAEFARPGGWNDPDMLEVGNGGMTKDEYIAHFSIWAISKAPLLLGCDVRNMSEDTMEIV 300

Query: 301 GNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIG 360
            N EVIA+NQDPLGVQ +KVR +GD+EVWAGPL+GYRVAVLL NR   R A  A+WDDIG
Sbjct: 301 ANKEVIAINQDPLGVQAKKVRMKGDLEVWAGPLTGYRVAVLLFNRGPSRSAISAYWDDIG 360

Query: 361 IPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
           IP +S+VQARD+W+HKTL ++FVGN++  +++H+CKMYIL PIS
Sbjct: 361 IPANSLVQARDVWEHKTLNREFVGNLTATVDSHACKMYILKPIS 404

BLAST of Lag0040627.1 vs. ExPASy TrEMBL
Match: A0A6J1CC22 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111010280 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.2e-178
Identity = 294/405 (72.59%), Postives = 341/405 (84.20%), Query Frame = 0

Query: 1   MEKLTIASILISCLLIAPSSSATSRKI-----DPENRRSLLANGLGLTPPMGWNSWNHFR 60
           M    +  ++ S +++A S SATSR       D   RR+LLANGLG+TPPMGWNSWNHF 
Sbjct: 12  MAMAVLVLVVYSSVMVA-SVSATSRMTEITADDELLRRNLLANGLGVTPPMGWNSWNHFA 71

Query: 61  CGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALS 120
           C INE ++K+ AD LISTGLSKLGY YVNIDDCWAE+ RD  G LVPKK+TFPSG+KAL+
Sbjct: 72  CNINEKMIKETADALISTGLSKLGYKYVNIDDCWAEIARDDKGNLVPKKSTFPSGMKALA 131

Query: 121 DYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPT 180
           DYVH+KGLKLGIYSDAG FTCS+TMPGSL HEEQDAKTFA WGIDYLKYDNCNN NIKPT
Sbjct: 132 DYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPT 191

Query: 181 ERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLN 240
            RYP+MTRALMKAGRPIF SLCEWGD+HPALW   +GNSWRTT+DI D+W+SM+SRADLN
Sbjct: 192 VRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLN 251

Query: 241 DAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEI 300
           + +A+ ARPGGWNDPDMLEVGNGGMTK+EY VHFS+WA+SKAPLLLG DLR ++ +T EI
Sbjct: 252 EVYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWALSKAPLLLGCDLRNLTKETKEI 311

Query: 301 IGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDI 360
           I N+EVIAVNQDPLGVQ +KVRSEGD+EVWAGPLS YRVAV+L+NR  WR    A WDDI
Sbjct: 312 ITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSSYRVAVVLLNRGPWRSVLTAQWDDI 371

Query: 361 GIPKDSVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
           GIP +S+VQARDLW+H TL+  FVGN+S  +++H+CKMYIL PIS
Sbjct: 372 GIPPNSIVQARDLWEHNTLKNVFVGNLSATVDSHACKMYILKPIS 415

BLAST of Lag0040627.1 vs. ExPASy TrEMBL
Match: A0A6P6GCI9 (Alpha-galactosidase OS=Ziziphus jujuba OX=326968 GN=LOC107421976 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 9.3e-178
Identity = 287/400 (71.75%), Postives = 339/400 (84.75%), Query Frame = 0

Query: 1   MEKLTIASILISCLLIAPSSSATSRKIDPENRRSLLANGLGLTPPMGWNSWNHFRCGINE 60
           M+ L++  +++ CL++   S+   +  D + RR+LLANGLG TPPMGWNSWNHF C INE
Sbjct: 1   MKTLSVMFVIL-CLMVMGVSARDVQ--DQQLRRNLLANGLGKTPPMGWNSWNHFSCNINE 60

Query: 61  TIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSDYVHS 120
            ++++ AD L+STGLSKLGY YVNIDDCWAE+ RD  G L+PKK+TFPSGIKAL+DYVHS
Sbjct: 61  KMIRETADALVSTGLSKLGYTYVNIDDCWAEIHRDAKGNLIPKKSTFPSGIKALADYVHS 120

Query: 121 KGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTERYPI 180
           KGLKLGIYSDAG FTCS+TMPGSL HEEQDAKTFA WGIDYLKYDNCNND  KPT RYP+
Sbjct: 121 KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNDGSKPTVRYPV 180

Query: 181 MTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLNDAFAE 240
           MTRALMKAGRPIF SLCEWGD+HPALW A +GNSWRTT+DI DNWDSM+SRAD+N+ +AE
Sbjct: 181 MTRALMKAGRPIFFSLCEWGDLHPALWGAKLGNSWRTTNDISDNWDSMLSRADMNEIYAE 240

Query: 241 HARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEIIGNSE 300
            ARPGGWNDPDMLEVGNGGMTK EY VHFS+WAISKAPLLLG D+R ++ DTMEII N E
Sbjct: 241 FARPGGWNDPDMLEVGNGGMTKNEYIVHFSLWAISKAPLLLGCDVRNVTKDTMEIITNKE 300

Query: 301 VIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIGIPKD 360
           VIAVNQD LGVQ +KVR EGD+E+WAG LSGYRVAV+L+NR  WR +  A WDDIGIP  
Sbjct: 301 VIAVNQDSLGVQAKKVRMEGDLEIWAGRLSGYRVAVVLLNRGPWRNSITAEWDDIGIPPK 360

Query: 361 SVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
           S+V+ARD+W+HKTL+ +FVGN++  ++ HSCKMY+L P+S
Sbjct: 361 SLVEARDVWEHKTLKTKFVGNLTATVDQHSCKMYVLKPVS 397

BLAST of Lag0040627.1 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 610.9 bits (1574), Expect = 7.2e-175
Identity = 280/402 (69.65%), Postives = 338/402 (84.08%), Query Frame = 0

Query: 3   KLTIASILISCL---LIAPSSSATSRKIDPE-NRRSLLANGLGLTPPMGWNSWNHFRCGI 62
           K+ I  ILIS +   ++  S S  +   D E  RR LL NGLG+TPPMGWNSWNHF C I
Sbjct: 9   KMPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNI 68

Query: 63  NETIVKQNADLLISTGLSKLGYVYVNIDDCWAELIRDVDGYLVPKKATFPSGIKALSDYV 122
           +E ++K+ AD L++TGLSKLGY YVNIDDCWAE+ RD  G LVPKK+TFPSGIKA++DYV
Sbjct: 69  DEKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYV 128

Query: 123 HSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAKTFAEWGIDYLKYDNCNNDNIKPTERY 182
           HSKGLKLGIYSDAG FTCS+TMPGSL +EE DAKTFAEWGIDYLKYDNCN+D  KPT RY
Sbjct: 129 HSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRY 188

Query: 183 PIMTRALMKAGRPIFLSLCEWGDMHPALWSADVGNSWRTTSDIKDNWDSMMSRADLNDAF 242
           P+MTRALMK+GRPIF SLCEWGDMHPALW + VGNSWRTT+DIKD W SM+S AD+N+ +
Sbjct: 189 PVMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVY 248

Query: 243 AEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIWAISKAPLLLGNDLREISDDTMEIIGN 302
           AEHARPGGWNDPDMLEVGNGGMTK+EY VHFSIWAISKAPLLLG D+R ++ +TMEI+ N
Sbjct: 249 AEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVAN 308

Query: 303 SEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGYRVAVLLVNRAGWRIAGRAHWDDIGIP 362
            EVIA+NQDP GVQ +KVR EGD+EVWAGPLSGYRVA+LL+NR   R +  A W+DI IP
Sbjct: 309 KEVIAINQDPHGVQAKKVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIP 368

Query: 363 KDSVVQARDLWQHKTLEKQFVGNISNILEAHSCKMYILTPIS 401
            +S+V+ARDLW+H+TL+++FVGN++  +++H+CK+Y+L P++
Sbjct: 369 ANSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLYVLKPVA 410

BLAST of Lag0040627.1 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 528.1 bits (1359), Expect = 6.2e-150
Identity = 242/368 (65.76%), Postives = 284/368 (77.17%), Query Frame = 0

Query: 33  RSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAEL 92
           R L+ NGL L+P MGWNSWNHF+C INET++KQ AD ++S+GLS +GY Y+NIDDCW EL
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 93  IRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAK 152
            RD  G LV K +TFPSGIKALSDYVHSKGLKLGIYSDAGT TCS+TMPGSL HEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 153 TFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVG 212
           TFA WGIDYLKYDNC N    P ERYP M++AL+ +GR IF SLCEWG   PA W+ D+G
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 213 NSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 272
           NSWRTT DI+DNW SM   AD ND +A +ARPG WNDPDMLEVGNGGMTKEEY  HFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 273 AISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGY 332
           A++KAPLL+G DLR +   T E++ N EVIAVNQD LG+QG+KV+ EGD+EVWAGPLS  
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

Query: 333 RVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKT---LEKQFVGNISNILEAH 392
           RVAV+L NR        A W +IG+    +V ARDLW+H T   ++KQ    +S ++E H
Sbjct: 329 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQ----LSALVEPH 388

Query: 393 SCKMYILT 398
           +CKMY LT
Sbjct: 389 ACKMYTLT 392

BLAST of Lag0040627.1 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 528.1 bits (1359), Expect = 6.2e-150
Identity = 242/368 (65.76%), Postives = 284/368 (77.17%), Query Frame = 0

Query: 33  RSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAEL 92
           R L+ NGL L+P MGWNSWNHF+C INET++KQ AD ++S+GLS +GY Y+NIDDCW EL
Sbjct: 3   RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 62

Query: 93  IRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAK 152
            RD  G LV K +TFPSGIKALSDYVHSKGLKLGIYSDAGT TCS+TMPGSL HEEQDAK
Sbjct: 63  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 122

Query: 153 TFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVG 212
           TFA WGIDYLKYDNC N    P ERYP M++AL+ +GR IF SLCEWG   PA W+ D+G
Sbjct: 123 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 182

Query: 213 NSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 272
           NSWRTT DI+DNW SM   AD ND +A +ARPG WNDPDMLEVGNGGMTKEEY  HFSIW
Sbjct: 183 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 242

Query: 273 AISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGDVEVWAGPLSGY 332
           A++KAPLL+G DLR +   T E++ N EVIAVNQD LG+QG+KV+ EGD+EVWAGPLS  
Sbjct: 243 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 302

Query: 333 RVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKT---LEKQFVGNISNILEAH 392
           RVAV+L NR        A W +IG+    +V ARDLW+H T   ++KQ    +S ++E H
Sbjct: 303 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQ----LSALVEPH 362

Query: 393 SCKMYILT 398
           +CKMY LT
Sbjct: 363 ACKMYTLT 366

BLAST of Lag0040627.1 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 490.0 bits (1260), Expect = 1.9e-138
Identity = 226/369 (61.25%), Postives = 270/369 (73.17%), Query Frame = 0

Query: 33  RSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAEL 92
           R  L NGL  TP MGWNSWN F C INET++K+ AD L+S+GL+ LGY++VNIDDCW+ L
Sbjct: 62  RLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNL 121

Query: 93  IRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAK 152
           +RD +G LVP   TFPSGIK L+DYVHSKGLKLGIYSDAG FTC    PGSL+HE  DA 
Sbjct: 122 LRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDAD 181

Query: 153 TFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVG 212
            FA WG+DYLKYDNC N  IKP ERYP M  AL   GR IF SLCEWG   PALW+ +VG
Sbjct: 182 IFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVG 241

Query: 213 NSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 272
           NSWRTT DI D W SM + ADLN+ +A +A PGGWNDPDMLE+GNGGMT EEYR HFSIW
Sbjct: 242 NSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIW 301

Query: 273 AISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGD---VEVWAGPL 332
           A+ KAPLL+G D+R ++ +T+EI+ N E+IAVNQDPLGVQGRK+++ G+    +VW+GPL
Sbjct: 302 ALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPL 361

Query: 333 SGYRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKTLEKQFVGNISNILEAH 392
           SG R+ V L NR        A WD IG+     V  RDLWQHK + +   G+    ++AH
Sbjct: 362 SGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAH 421

Query: 393 SCKMYILTP 399
            C MY+LTP
Sbjct: 422 DCHMYVLTP 429

BLAST of Lag0040627.1 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 440.7 bits (1132), Expect = 1.3e-123
Identity = 211/369 (57.18%), Postives = 252/369 (68.29%), Query Frame = 0

Query: 33  RSLLANGLGLTPPMGWNSWNHFRCGINETIVKQNADLLISTGLSKLGYVYVNIDDCWAEL 92
           R  L NGL  TP MGWNSWN F C INET++K+ AD L+S+GL+ LGY++VNI       
Sbjct: 62  RLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNI------- 121

Query: 93  IRDVDGYLVPKKATFPSGIKALSDYVHSKGLKLGIYSDAGTFTCSRTMPGSLYHEEQDAK 152
                            GIK L+DYVHSKGLKLGIYSDAG FTC    PGSL+HE  DA 
Sbjct: 122 -----------------GIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDAD 181

Query: 153 TFAEWGIDYLKYDNCNNDNIKPTERYPIMTRALMKAGRPIFLSLCEWGDMHPALWSADVG 212
            FA WG+DYLKYDNC N  IKP ERYP M  AL   GR IF SLCEWG   PALW+ +VG
Sbjct: 182 IFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVG 241

Query: 213 NSWRTTSDIKDNWDSMMSRADLNDAFAEHARPGGWNDPDMLEVGNGGMTKEEYRVHFSIW 272
           NSWRTT DI D W SM + ADLN+ +A +A PGGWNDPDMLE+GNGGMT EEYR HFSIW
Sbjct: 242 NSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIW 301

Query: 273 AISKAPLLLGNDLREISDDTMEIIGNSEVIAVNQDPLGVQGRKVRSEGD---VEVWAGPL 332
           A+ KAPLL+G D+R ++ +T+EI+ N E+IAVNQDPLGVQGRK+++ G+    +VW+GPL
Sbjct: 302 ALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPL 361

Query: 333 SGYRVAVLLVNRAGWRIAGRAHWDDIGIPKDSVVQARDLWQHKTLEKQFVGNISNILEAH 392
           SG R+ V L NR        A WD IG+     V  RDLWQHK + +   G+    ++AH
Sbjct: 362 SGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAH 405

Query: 393 SCKMYILTP 399
            C MY+LTP
Sbjct: 422 DCHMYVLTP 405