Lag0040217.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0040217.1 vs. NCBI nr
Match: XP_022956862.1 (protein PHYLLO, chloroplastic isoform X1 [Cucurbita moschata] >KAG7032137.1 Protein PHYLLO, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3119.7 bits (8087), Expect = 0.0e+00
Identity = 1559/1721 (90.59%), Postives = 1628/1721 (94.60%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+LSI   ALPHPVRPF R SFPRS LFSTF F PK +  PGSFRLPQ SCFKGVR+
Sbjct: 1    MKADTLSIHNPALPHPVRPFNRASFPRSNLFSTFGFIPKPSRHPGSFRLPQTSCFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GATELS+LEDV+L+VEMCIT T+PPALTLKQGLESISEAI+KLKLE PRSS GVF
Sbjct: 61   DSPVMGATELSELEDVELMVEMCITHTLPPALTLKQGLESISEAIDKLKLESPRSSDGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS RALMWFCCQPESSE++PIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSERALMWFCCQPESSEMYPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQ TLKRYL+NDSAPIMAYGFVNANTGETSS+KN+A HSYFCVPQIEL EYEG
Sbjct: 181  SLSSTSSKQCTLKRYLMNDSAPIMAYGFVNANTGETSSLKNEAGHSYFCVPQIELNEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            +SVLSATLAWSE+FPCTFEE LHSLG SI+QI TNFPS+E  QYKYIRSSLT+ KL+D T
Sbjct: 241  VSVLSATLAWSEAFPCTFEEALHSLGSSIYQIGTNFPSKENFQYKYIRSSLTASKLVDVT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEM-ESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEH 360
            HQM YMKV SVAGESFRTGIMEM ESA SHQFCIRLSPSVAVACNMLD  NET  S QEH
Sbjct: 301  HQMGYMKVFSVAGESFRTGIMEMKESARSHQFCIRLSPSVAVACNMLDRTNETCLSEQEH 360

Query: 361  TNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAI 420
             NI+ALWAS IVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCI+CFDERSLAFHAI
Sbjct: 361  ANINALWASFIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCISCFDERSLAFHAI 420

Query: 421  GYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQV 480
            GYAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQDAGANQAINQV
Sbjct: 421  GYAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQDAGANQAINQV 480

Query: 481  NHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSP 540
            NHFGSFVRFFF+LP PTD+IPARMVLTTLDSAVHWATS+PCGPVHINCPFREPLENSPSP
Sbjct: 481  NHFGSFVRFFFSLPAPTDEIPARMVLTTLDSAVHWATSSPCGPVHINCPFREPLENSPSP 540

Query: 541  WNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHS 600
            WNL+ LNGL  WLSS+EAFTKYI+LEASP SIDTFG MAEVLK+IHGARNGILLLGSI S
Sbjct: 541  WNLSSLNGLQNWLSSSEAFTKYIRLEASPMSIDTFGHMAEVLKVIHGARNGILLLGSIQS 600

Query: 601  EDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKW 660
            EDEIWAA LLAKHISWPIVADVLSGLRLRKSLSSFLE+E NFFFVDHLDHALLSDSVRKW
Sbjct: 601  EDEIWAAFLLAKHISWPIVADVLSGLRLRKSLSSFLEVENNFFFVDHLDHALLSDSVRKW 660

Query: 661  LKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720
            LKFDVIIQ+GSRVTSKRVSKMLEDC+PCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC
Sbjct: 661  LKFDVIIQMGSRVTSKRVSKMLEDCSPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720

Query: 721  LLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLG 780
            LL+ GFPRNRS WTATL ALNMMVE EIQFQISANY LSEPQVAQ+ISEALP DSVLFLG
Sbjct: 721  LLKTGFPRNRSKWTATLLALNMMVEWEIQFQISANYCLSEPQVAQVISEALPFDSVLFLG 780

Query: 781  NSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVG 840
            NSMPIRDVDMYA GWSKC DSVA RPLNLQ+PF W WTSGNRGASGIDGLLSTAVGFSVG
Sbjct: 781  NSMPIRDVDMYARGWSKCTDSVAVRPLNLQLPFCWTWTSGNRGASGIDGLLSTAVGFSVG 840

Query: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAIL 900
            CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKV+ AIL
Sbjct: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVEAAIL 900

Query: 901  DQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTF 960
            DQ+FHTSH+ISLRSLC+AHGLKHLHVRT MELQDALFMSQ EENDCIIEVESSIDANT+F
Sbjct: 901  DQFFHTSHKISLRSLCVAHGLKHLHVRTKMELQDALFMSQCEENDCIIEVESSIDANTSF 960

Query: 961  HSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSI 1020
            HSVLRKFTCQAVDHGLR+FSRLYFEESVSPGLFLCKISRM+YTLFRIPLCAP TT+SSSI
Sbjct: 961  HSVLRKFTCQAVDHGLRIFSRLYFEESVSPGLFLCKISRMEYTLFRIPLCAPTTTSSSSI 1020

Query: 1021 DQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSF 1080
            DQV+R FFREGFILSLFLEDGSLGLGEVSPLEIH+ENLLDVEEQLKCLLHI KGAKISS 
Sbjct: 1021 DQVNRGFFREGFILSLFLEDGSLGLGEVSPLEIHKENLLDVEEQLKCLLHIFKGAKISSS 1080

Query: 1081 IPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENN 1140
            IPLLRGSFSSWIFHELGI P SIYPSVRCGLEMAVLNAIAGRKGCSLL+VLQ++L EE++
Sbjct: 1081 IPLLRGSFSSWIFHELGIHPRSIYPSVRCGLEMAVLNAIAGRKGCSLLDVLQHRLVEEDD 1140

Query: 1141 LTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKK 1200
            L+SSSKV+ICGLLDSGG+PS VARVAKTLVEEGF AIKLKVARQGNVMYDAAVVQEVRKK
Sbjct: 1141 LSSSSKVKICGLLDSGGTPSEVARVAKTLVEEGFPAIKLKVARQGNVMYDAAVVQEVRKK 1200

Query: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260
            LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE
Sbjct: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260

Query: 1261 TVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETG 1320
            T+D IQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFE+G
Sbjct: 1261 TIDGIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFESG 1320

Query: 1321 VGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSG 1380
            VGLSGYI LS YLEL NAE+RKLM+IQPAPSIAHGLGT+RWL+EDVTVNPLRI RDP SG
Sbjct: 1321 VGLSGYIHLSCYLELQNAEIRKLMDIQPAPSIAHGLGTFRWLDEDVTVNPLRIGRDPHSG 1380

Query: 1381 IIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDN 1440
            IIEASVAEANRLLK+FQINQK VCRK TEEQVRRY+LTVD KGFSYLIKVLEVG RTNDN
Sbjct: 1381 IIEASVAEANRLLKSFQINQKVVCRKFTEEQVRRYKLTVDSKGFSYLIKVLEVGQRTNDN 1440

Query: 1441 VLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEV 1500
            VL+FLHGFLGTGEDWITIMKA+SGSARCIS+DLPGHG STTQKNDCDVH VEEPSFSMEV
Sbjct: 1441 VLLFLHGFLGTGEDWITIMKAISGSARCISIDLPGHGESTTQKNDCDVHGVEEPSFSMEV 1500

Query: 1501 VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARRI 1560
            VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRF DKIGRA IISGSPGLKDKMARRI
Sbjct: 1501 VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFADKIGRAAIISGSPGLKDKMARRI 1560

Query: 1561 RRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKALS 1620
            RRVKDDSRARILK YGL+SFLE WY GELWKSLREHPHYS+IIASR KHDDVQHLAKALS
Sbjct: 1561 RRVKDDSRARILKAYGLESFLEAWYRGELWKSLREHPHYSQIIASRSKHDDVQHLAKALS 1620

Query: 1621 DLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELHEI 1680
            DLSIGRQPQLWDELK CKTPLSIIVGEKDTKFKTIAQ+ILSQIDMGRKIKD+PVV LHEI
Sbjct: 1621 DLSIGRQPQLWDELKCCKTPLSIIVGEKDTKFKTIAQQILSQIDMGRKIKDDPVVSLHEI 1680

Query: 1681 VEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            VEIPDSGHAAHLENPLAVINALSKFLIRSRTQ SYNSDFSG
Sbjct: 1681 VEIPDSGHAAHLENPLAVINALSKFLIRSRTQLSYNSDFSG 1721

BLAST of Lag0040217.1 vs. NCBI nr
Match: XP_038893377.1 (protein PHYLLO, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 3113.9 bits (8072), Expect = 0.0e+00
Identity = 1541/1724 (89.39%), Postives = 1628/1724 (94.43%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M ADSL I KLA+ HPVRPFI  SFPRSKLFS+FA  PKG+ QP SFRLPQNSCFK VR+
Sbjct: 1    MQADSLRIHKLAVLHPVRPFITDSFPRSKLFSSFALIPKGSRQPASFRLPQNSCFKCVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GATELSDLEDV+ +VEMCIT T+PPALTLKQGLESISEAIEKLKLEPPRSS GVF
Sbjct: 61   DSPVMGATELSDLEDVEFLVEMCITHTLPPALTLKQGLESISEAIEKLKLEPPRSSHGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPSTRAL+WFCCQPESSEV+PIFFLS EKDATIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSTRALLWFCCQPESSEVYPIFFLSNEKDATIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            +LSSTSSK+STLKRYL+NDSAPIMAYGFVN NTGE SS+KN+A HS FC+PQIEL EYEG
Sbjct: 181  NLSSTSSKRSTLKRYLMNDSAPIMAYGFVNDNTGEISSLKNEAGHSCFCIPQIELSEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            ISVLSATLAWSESFPCTFEE +HSLG SI+QISTNFPS+  CQYKYIRSSLT+ KL+D T
Sbjct: 241  ISVLSATLAWSESFPCTFEEAIHSLGSSIYQISTNFPSKGNCQYKYIRSSLTASKLVDRT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEMESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEHT 360
            +QMAYMKVLSVAGESFRTGIMEMESA SHQFCIR+SP+VAVACNMLDHINE  HS QEH 
Sbjct: 301  NQMAYMKVLSVAGESFRTGIMEMESALSHQFCIRVSPNVAVACNMLDHINENVHSEQEHA 360

Query: 361  NISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG 420
            NI+A+WASLIVEECSRLGLTYFCIAPGSRSSPLA+AAASHPLITCIACFDERSLAFHAIG
Sbjct: 361  NINAVWASLIVEECSRLGLTYFCIAPGSRSSPLAVAAASHPLITCIACFDERSLAFHAIG 420

Query: 421  YAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQVN 480
            YAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQDAGANQAINQVN
Sbjct: 421  YAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQDAGANQAINQVN 480

Query: 481  HFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSPW 540
            HFGSFVRFFF+LP PTDQIPARMVLTTLDSAVHWATS+PCGPVHINCPFREPLENSPSPW
Sbjct: 481  HFGSFVRFFFSLPAPTDQIPARMVLTTLDSAVHWATSSPCGPVHINCPFREPLENSPSPW 540

Query: 541  NLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHSE 600
            NL+CLNGLHIWLSSTE FTKYI+LEASPTSIDTFG MAEVLK+IHGA+NGILLLGSI SE
Sbjct: 541  NLSCLNGLHIWLSSTEVFTKYIRLEASPTSIDTFGHMAEVLKVIHGAKNGILLLGSIQSE 600

Query: 601  DEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKWL 660
            DEIWAA LLAKHISWPIVADVLSGLRLRKSL+SFLE+E NFFFVDHLDHALLSD VRKWL
Sbjct: 601  DEIWAAFLLAKHISWPIVADVLSGLRLRKSLTSFLEMENNFFFVDHLDHALLSDLVRKWL 660

Query: 661  KFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGCL 720
            KFDVIIQIGSRVTSKR+SKMLEDC+PCSYIMVD++PGRHDPSHIVTHRIQSTVLEFVGCL
Sbjct: 661  KFDVIIQIGSRVTSKRISKMLEDCSPCSYIMVDKYPGRHDPSHIVTHRIQSTVLEFVGCL 720

Query: 721  LEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLGN 780
            L+ GFP +RS WT TL+ALNMMVE EIQFQISANYSLSEPQVAQ+ISEALP DSVLFLGN
Sbjct: 721  LKAGFPLSRSKWTTTLRALNMMVEWEIQFQISANYSLSEPQVAQVISEALPCDSVLFLGN 780

Query: 781  SMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVGC 840
            SMPIRDVDMYAYGWSKCNDSVAAR LNLQ+PF W WTSGNRGASGIDGLLSTAVGFSVGC
Sbjct: 781  SMPIRDVDMYAYGWSKCNDSVAARQLNLQLPFCWTWTSGNRGASGIDGLLSTAVGFSVGC 840

Query: 841  NKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAILD 900
            NKRVLC+VGD+SFLHDTNGLAILNQRM RKPVTVVVINNNGGAIFSLLPIKDKV+ AILD
Sbjct: 841  NKRVLCLVGDISFLHDTNGLAILNQRMARKPVTVVVINNNGGAIFSLLPIKDKVEAAILD 900

Query: 901  QYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTFH 960
            Q+FHTSH++SLRSLCMAHGLKHLHVRT MELQDALF S  EENDCIIEV+SSIDANTTFH
Sbjct: 901  QFFHTSHKVSLRSLCMAHGLKHLHVRTKMELQDALFQSHHEENDCIIEVDSSIDANTTFH 960

Query: 961  SVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSID 1020
            SVLRKFTCQAVDHGLR+FS L+ EESVS GLFLCKISRM+ TLFRIPLCAPPTT SSSID
Sbjct: 961  SVLRKFTCQAVDHGLRIFSGLHSEESVSSGLFLCKISRMECTLFRIPLCAPPTTASSSID 1020

Query: 1021 QVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSFI 1080
            QVSRKFFREGFILSL+LEDGSLGLGE+SPL+IHRE+LLDVEEQLKCL+HILKGAKI S I
Sbjct: 1021 QVSRKFFREGFILSLYLEDGSLGLGEISPLDIHRESLLDVEEQLKCLIHILKGAKIRSSI 1080

Query: 1081 PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENNL 1140
            PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLL+VLQ+QLDEE NL
Sbjct: 1081 PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLDVLQHQLDEEKNL 1140

Query: 1141 TSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKKL 1200
             +SSKVQICGLLDSGG+PS VA VAKTL EEGF  IKLKVARQGNVMYDAAVVQE+RKKL
Sbjct: 1141 ATSSKVQICGLLDSGGTPSEVAHVAKTLAEEGFPTIKLKVARQGNVMYDAAVVQEIRKKL 1200

Query: 1201 GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDET 1260
            GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAI+KFCEESGLPVALDET
Sbjct: 1201 GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIIKFCEESGLPVALDET 1260

Query: 1261 VDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETGV 1320
            +DHIQDNPVKELAKY HPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFE+GV
Sbjct: 1261 LDHIQDNPVKELAKYAHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFESGV 1320

Query: 1321 GLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSGI 1380
            GLSGYI LS YLEL NAE+RKLMNIQPAPSIAHGLGTYRWLEEDVT++PLRI RDP SGI
Sbjct: 1321 GLSGYIHLSCYLELQNAEVRKLMNIQPAPSIAHGLGTYRWLEEDVTLDPLRISRDPHSGI 1380

Query: 1381 IEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDNV 1440
            +EASVAEANRLLK FQINQKFVC+K TEEQV RY+L+VD KGFSY  KVLEVG RTNDNV
Sbjct: 1381 VEASVAEANRLLKGFQINQKFVCKKFTEEQVCRYKLSVDSKGFSYSTKVLEVGQRTNDNV 1440

Query: 1441 LVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEVV 1500
            L FLHGFLGTGEDWITIMK +SGSARCISLDLPGHG STTQKNDCDVH VEEPS+S+EV+
Sbjct: 1441 LFFLHGFLGTGEDWITIMKGISGSARCISLDLPGHGESTTQKNDCDVHGVEEPSYSIEVI 1500

Query: 1501 ANLLYKLIHHLAPGK--VNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR 1560
            A+LLYKLIHHLAPGK  VNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR
Sbjct: 1501 ADLLYKLIHHLAPGKGIVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR 1560

Query: 1561 IRRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKAL 1620
            IRRVKDDSRARILKVYGLQ FLE WYCGELWKSLREHPHYS+IIASRLKHDDVQHLAKAL
Sbjct: 1561 IRRVKDDSRARILKVYGLQRFLEAWYCGELWKSLREHPHYSQIIASRLKHDDVQHLAKAL 1620

Query: 1621 SDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELHE 1680
            SDLSIGRQPQLWDELK CK PLSIIVGEKDTKFKTIAQ+ILSQIDMG+KIKDEPVV+LHE
Sbjct: 1621 SDLSIGRQPQLWDELKCCKIPLSIIVGEKDTKFKTIAQQILSQIDMGKKIKDEPVVDLHE 1680

Query: 1681 IVEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSGLG 1723
            IVEIPDSGHAAHLENPL VINALS+FLIRSRT+ SYN DF+G G
Sbjct: 1681 IVEIPDSGHAAHLENPLDVINALSRFLIRSRTRCSYNPDFAGRG 1724

BLAST of Lag0040217.1 vs. NCBI nr
Match: XP_023511862.1 (protein PHYLLO, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3112.8 bits (8069), Expect = 0.0e+00
Identity = 1554/1721 (90.30%), Postives = 1628/1721 (94.60%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+LSI   ALPHPVRPF R SFPRS LFST  F PK +  PGSFRLP  SCFKGVR+
Sbjct: 1    MKADTLSIHNPALPHPVRPFNRASFPRSILFSTSGFIPKPSRHPGSFRLPHTSCFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GATELS+LEDV+L+VEMCIT T+PPALTLKQGLESISEAI+KLK E PRSS GVF
Sbjct: 61   DSPVMGATELSELEDVELMVEMCITHTLPPALTLKQGLESISEAIDKLKFESPRSSDGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS RALMWFCCQPESSEV+PIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSARALMWFCCQPESSEVYPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQ TLKRYL+NDSAPIMAYGFVNANTG TSS+KN+A HSYFCVPQIEL EYEG
Sbjct: 181  SLSSTSSKQCTLKRYLMNDSAPIMAYGFVNANTGATSSLKNEAGHSYFCVPQIELNEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            +SVLSATLAWSE+FPCTFEE LHSLG SI+QI TNFPS+E  QYKYIRSSLT+ KL++ T
Sbjct: 241  VSVLSATLAWSEAFPCTFEEALHSLGSSIYQIGTNFPSKENFQYKYIRSSLTASKLVNVT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEM-ESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEH 360
            HQM YMKV SVAG+SFRTGIMEM ESA SHQFCIRLSPSVAVACNMLD  NET  S QEH
Sbjct: 301  HQMGYMKVFSVAGKSFRTGIMEMKESARSHQFCIRLSPSVAVACNMLDRTNETCLSEQEH 360

Query: 361  TNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAI 420
             NI+ALWAS IVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAI
Sbjct: 361  ANINALWASFIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAI 420

Query: 421  GYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQV 480
            GYAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQDAGANQAINQV
Sbjct: 421  GYAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQDAGANQAINQV 480

Query: 481  NHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSP 540
            NHFGSFVRFFF+LP PTD+IPARMVLTTLDSAVHWATS+PCGPVHINCPFREPLENSPSP
Sbjct: 481  NHFGSFVRFFFSLPAPTDEIPARMVLTTLDSAVHWATSSPCGPVHINCPFREPLENSPSP 540

Query: 541  WNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHS 600
            WNL+ LNGL  WLSS+EAFTKYI+LEASP SIDTFG MAEVLK+IHGARNGILLLGSI S
Sbjct: 541  WNLSSLNGLQNWLSSSEAFTKYIRLEASPMSIDTFGHMAEVLKVIHGARNGILLLGSIQS 600

Query: 601  EDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKW 660
            EDEIWAA LLAKHISWPIVADVLSGLRLRKSLSSFLE+E NFFFVDHLDHALLSDSVRKW
Sbjct: 601  EDEIWAAFLLAKHISWPIVADVLSGLRLRKSLSSFLEVENNFFFVDHLDHALLSDSVRKW 660

Query: 661  LKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720
            LKFDVIIQ+GSRVTSKRVSKMLEDC+PCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC
Sbjct: 661  LKFDVIIQMGSRVTSKRVSKMLEDCSPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720

Query: 721  LLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLG 780
            LL+ GFPRNRS WTATLQALNMMVE EIQFQISANY LSEPQVAQ+ISEALP DSVLFLG
Sbjct: 721  LLKTGFPRNRSKWTATLQALNMMVEWEIQFQISANYCLSEPQVAQVISEALPFDSVLFLG 780

Query: 781  NSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVG 840
            NSMPIRDVDMYA GWSKC DSVA RPLNLQ+PF W WTSGNRGASGIDGLLSTAVGFSVG
Sbjct: 781  NSMPIRDVDMYARGWSKCTDSVAVRPLNLQLPFCWTWTSGNRGASGIDGLLSTAVGFSVG 840

Query: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAIL 900
            CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKV+ AIL
Sbjct: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVEAAIL 900

Query: 901  DQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTF 960
            DQ+FHTSH+ISLRSLC+AHGLKHLHVRT MELQDALFMSQ EENDCIIEVESSIDANT+F
Sbjct: 901  DQFFHTSHKISLRSLCVAHGLKHLHVRTKMELQDALFMSQCEENDCIIEVESSIDANTSF 960

Query: 961  HSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSI 1020
            HSVLRKFTCQAVDHGLR+FSRLYFEESVSPGLFLCKISRM+YTLFRIPLCAPPTT+ +SI
Sbjct: 961  HSVLRKFTCQAVDHGLRIFSRLYFEESVSPGLFLCKISRMEYTLFRIPLCAPPTTSCNSI 1020

Query: 1021 DQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSF 1080
            DQV+R FFREGF+LSLFLEDGSLGLGEVSPLEIH+ENLLDVEEQLKCLLH+LKGAKISS 
Sbjct: 1021 DQVNRSFFREGFVLSLFLEDGSLGLGEVSPLEIHKENLLDVEEQLKCLLHMLKGAKISSS 1080

Query: 1081 IPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENN 1140
            IPLLRGSFSSWIFHELGIPP SIYPSVRCGLEMAVLNAIAGRKGCSLL+VLQ++L EE++
Sbjct: 1081 IPLLRGSFSSWIFHELGIPPRSIYPSVRCGLEMAVLNAIAGRKGCSLLDVLQHRLVEEDD 1140

Query: 1141 LTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKK 1200
            L+SSSKV+ICGLLDSGG+PS VARVAKTLVEEGF AIKLKVARQGNVMYDAAVVQEVRKK
Sbjct: 1141 LSSSSKVKICGLLDSGGTPSEVARVAKTLVEEGFPAIKLKVARQGNVMYDAAVVQEVRKK 1200

Query: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260
            LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE
Sbjct: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260

Query: 1261 TVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETG 1320
            T+D IQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFE+G
Sbjct: 1261 TIDGIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFESG 1320

Query: 1321 VGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSG 1380
            VGLSGYI LS YLEL NAE+RKLM+IQPAPSIAHGLGT+RWL+EDVTVNPLRI RDP SG
Sbjct: 1321 VGLSGYIHLSCYLELQNAEIRKLMDIQPAPSIAHGLGTFRWLDEDVTVNPLRIGRDPHSG 1380

Query: 1381 IIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDN 1440
            IIEASVAEANRLLK+FQINQKFVCRK TEEQV RY+LTVD KGFSYLIKVLEVG RTNDN
Sbjct: 1381 IIEASVAEANRLLKSFQINQKFVCRKFTEEQVCRYKLTVDSKGFSYLIKVLEVGQRTNDN 1440

Query: 1441 VLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEV 1500
            VL+FLHGFLGTGEDWITIMKA+SGSARCIS+DLPGHG STTQKNDCDVH VEEPSFSMEV
Sbjct: 1441 VLLFLHGFLGTGEDWITIMKAISGSARCISIDLPGHGESTTQKNDCDVHGVEEPSFSMEV 1500

Query: 1501 VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARRI 1560
            VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRF DKIGRA IISGSPGLKDKMARRI
Sbjct: 1501 VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFADKIGRAAIISGSPGLKDKMARRI 1560

Query: 1561 RRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKALS 1620
            RRVKDDSRARILK YGL+SFLE WY GELWKSLREHPHYS+IIASR KHDDVQHLAKALS
Sbjct: 1561 RRVKDDSRARILKAYGLESFLEAWYSGELWKSLREHPHYSQIIASRSKHDDVQHLAKALS 1620

Query: 1621 DLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELHEI 1680
            DLSIGRQPQLWDELK CKTPLSIIVGEKDTKFKTIAQ+ILSQIDMGRKIKDEP+V+LHEI
Sbjct: 1621 DLSIGRQPQLWDELKCCKTPLSIIVGEKDTKFKTIAQQILSQIDMGRKIKDEPIVDLHEI 1680

Query: 1681 VEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            VEIPDSGHAAHLENPLAVINALSKFLIRSRTQF YNSDF+G
Sbjct: 1681 VEIPDSGHAAHLENPLAVINALSKFLIRSRTQF-YNSDFAG 1720

BLAST of Lag0040217.1 vs. NCBI nr
Match: KAG6601351.1 (Protein PHYLLO, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3110.1 bits (8062), Expect = 0.0e+00
Identity = 1559/1735 (89.86%), Postives = 1628/1735 (93.83%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+LSI   ALPHPVRPF R SFPRS LFSTF F PK +  PGSFRLPQ SCFKGVR+
Sbjct: 1    MKADTLSIHNPALPHPVRPFNRASFPRSNLFSTFGFIPKPSRHPGSFRLPQTSCFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GATELS+LEDV+L+VEMCIT T+PPALTLKQGLESISEAI+KLKLE PRSS GVF
Sbjct: 61   DSPVMGATELSELEDVELMVEMCITHTLPPALTLKQGLESISEAIDKLKLESPRSSDGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS RALMWFCCQPESSE++PIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSERALMWFCCQPESSEMYPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQ TLKRYL+NDSAPIMAYGFVNANTGETSS+KN+A HSYFCVPQIEL EYEG
Sbjct: 181  SLSSTSSKQCTLKRYLMNDSAPIMAYGFVNANTGETSSLKNEAGHSYFCVPQIELNEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            +SVLSATLAWSE+FPCTFEE LHSLG SI+QI TNFPS+E  QYKYIRSSLT+ KL+D T
Sbjct: 241  VSVLSATLAWSEAFPCTFEEALHSLGSSIYQIGTNFPSKENFQYKYIRSSLTASKLVDVT 300

Query: 301  HQM--------------AYMKVLSVAGESFRTGIMEM-ESAFSHQFCIRLSPSVAVACNM 360
            HQM               YMKV SVAGESFRTGIMEM ESA SHQFCIRLSPSVAVACNM
Sbjct: 301  HQMTLVLTWTCLAILTKGYMKVFSVAGESFRTGIMEMKESARSHQFCIRLSPSVAVACNM 360

Query: 361  LDHINETRHSMQEHTNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITC 420
            LD  NET  S QEH NI+ALWAS IVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITC
Sbjct: 361  LDRTNETCLSEQEHANINALWASFIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITC 420

Query: 421  IACFDERSLAFHAIGYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPP 480
            I+CFDERSLAFHAIGYAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP 
Sbjct: 421  ISCFDERSLAFHAIGYAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPL 480

Query: 481  ELQDAGANQAINQVNHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHI 540
            ELQDAGANQAINQVNHFGSFVRFFF+LP PTD+IPARMVLTTLDSAVHWATS+PCGPVHI
Sbjct: 481  ELQDAGANQAINQVNHFGSFVRFFFSLPAPTDEIPARMVLTTLDSAVHWATSSPCGPVHI 540

Query: 541  NCPFREPLENSPSPWNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIH 600
            NCPFREPLENSPSPWNL+ LNGL  WLSS+EAFTKYI+LEASP SIDTFG MAEVLK+IH
Sbjct: 541  NCPFREPLENSPSPWNLSSLNGLQNWLSSSEAFTKYIRLEASPMSIDTFGHMAEVLKVIH 600

Query: 601  GARNGILLLGSIHSEDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVD 660
            GARNGILLLGSI SEDEIWAA LLAKHISWPIVADVLSGLRLRKSLSSFLE+E NFFFVD
Sbjct: 601  GARNGILLLGSIQSEDEIWAAFLLAKHISWPIVADVLSGLRLRKSLSSFLEVENNFFFVD 660

Query: 661  HLDHALLSDSVRKWLKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIV 720
            HLDHALLSDSVRKWLKFDVIIQ+GSRVTSKRVSKMLEDC+PCSYIMVDEHPGRHDPSHIV
Sbjct: 661  HLDHALLSDSVRKWLKFDVIIQMGSRVTSKRVSKMLEDCSPCSYIMVDEHPGRHDPSHIV 720

Query: 721  THRIQSTVLEFVGCLLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQI 780
            THRIQSTVLEFVGCLL+ GFPRNRS WTATL ALNMMVE EIQFQISANY LSEPQVAQ+
Sbjct: 721  THRIQSTVLEFVGCLLKTGFPRNRSKWTATLLALNMMVEWEIQFQISANYCLSEPQVAQV 780

Query: 781  ISEALPSDSVLFLGNSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASG 840
            ISEALP DSVLFLGNSMPIRDVDMYA GWSKC DSVA RPLNLQ+PF W WTSGNRGASG
Sbjct: 781  ISEALPFDSVLFLGNSMPIRDVDMYARGWSKCTDSVAVRPLNLQLPFCWTWTSGNRGASG 840

Query: 841  IDGLLSTAVGFSVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIF 900
            IDGLLSTAVGFSVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIF
Sbjct: 841  IDGLLSTAVGFSVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIF 900

Query: 901  SLLPIKDKVDPAILDQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDC 960
            SLLPIKDKV+ AILDQ+FHTSH+ISLRSLC+AHGLKHLHVRT MELQDALFMSQ EENDC
Sbjct: 901  SLLPIKDKVEAAILDQFFHTSHKISLRSLCVAHGLKHLHVRTKMELQDALFMSQCEENDC 960

Query: 961  IIEVESSIDANTTFHSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFR 1020
            IIEVESSIDANT+FHSVLRKFTCQAVDHGLR+FSRLYFEESVSPGLFLCKISRM+YTLFR
Sbjct: 961  IIEVESSIDANTSFHSVLRKFTCQAVDHGLRIFSRLYFEESVSPGLFLCKISRMEYTLFR 1020

Query: 1021 IPLCAPPTTTSSSIDQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLK 1080
            IPLCAP TT+SSSIDQV+R FFREGFILSLFLEDGSLGLGEVSPLEIH+ENLLDVEEQLK
Sbjct: 1021 IPLCAPTTTSSSSIDQVNRGFFREGFILSLFLEDGSLGLGEVSPLEIHKENLLDVEEQLK 1080

Query: 1081 CLLHILKGAKISSFIPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCS 1140
            CLLHI KGAKISS IPLLRGSFSSWIFHELGI P SIYPSVRCGLEMAVLNAIAGRKGCS
Sbjct: 1081 CLLHIFKGAKISSSIPLLRGSFSSWIFHELGIHPRSIYPSVRCGLEMAVLNAIAGRKGCS 1140

Query: 1141 LLNVLQYQLDEENNLTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGN 1200
            LL+VLQ++L EE++L+SSSKV+ICGLLDSGG+PS VARVAKTLVEEGF AIKLKVARQGN
Sbjct: 1141 LLDVLQHRLVEEDDLSSSSKVKICGLLDSGGTPSEVARVAKTLVEEGFPAIKLKVARQGN 1200

Query: 1201 VMYDAAVVQEVRKKLGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIV 1260
            VMYDAAVVQEVRKKLGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIV
Sbjct: 1201 VMYDAAVVQEVRKKLGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIV 1260

Query: 1261 KFCEESGLPVALDETVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQ 1320
            KFCEESGLPVALDET+D IQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQ
Sbjct: 1261 KFCEESGLPVALDETIDGIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQ 1320

Query: 1321 HGKMAVVSAAFETGVGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDV 1380
            HGKMAVVSAAFE+GVGLSGYI LS YLEL NAE+RKLM+IQPAPSIAHGLGT+RWL+EDV
Sbjct: 1321 HGKMAVVSAAFESGVGLSGYIHLSCYLELQNAEIRKLMDIQPAPSIAHGLGTFRWLDEDV 1380

Query: 1381 TVNPLRICRDPCSGIIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSY 1440
            TVNPLRI RDP SGIIEASVAEANRLLK+FQINQK VCRK TEEQVRRY+LTVD KGFSY
Sbjct: 1381 TVNPLRIGRDPHSGIIEASVAEANRLLKSFQINQKVVCRKFTEEQVRRYKLTVDSKGFSY 1440

Query: 1441 LIKVLEVGPRTNDNVLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDC 1500
            LIKVLEVG RTNDNVL+FLHGFLGTGEDWITIMKA+SGSARCIS+DLPGHG STTQKNDC
Sbjct: 1441 LIKVLEVGQRTNDNVLLFLHGFLGTGEDWITIMKAISGSARCISIDLPGHGESTTQKNDC 1500

Query: 1501 DVHDVEEPSFSMEVVANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFGDKIGRAVII 1560
            DVH VEEPSFSMEVVANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRF DKIGRA II
Sbjct: 1501 DVHGVEEPSFSMEVVANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFADKIGRAAII 1560

Query: 1561 SGSPGLKDKMARRIRRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASR 1620
            SGSPGLKDKMARRIRRVKDDSRARILK YGL+SFLE WY GELWKSLREHPHYS+IIASR
Sbjct: 1561 SGSPGLKDKMARRIRRVKDDSRARILKAYGLESFLEAWYRGELWKSLREHPHYSQIIASR 1620

Query: 1621 LKHDDVQHLAKALSDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMG 1680
             KHDDVQHLAKALSDLSIGRQPQLWDELK CKTPLSIIVGEKDTKFKTIAQ+ILSQIDMG
Sbjct: 1621 SKHDDVQHLAKALSDLSIGRQPQLWDELKCCKTPLSIIVGEKDTKFKTIAQQILSQIDMG 1680

Query: 1681 RKIKDEPVVELHEIVEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            RKIKD+PVV LHEIVEIPDSGHAAHLENPLAVINALSKFLIRSRTQ SYNSDFSG
Sbjct: 1681 RKIKDDPVVSLHEIVEIPDSGHAAHLENPLAVINALSKFLIRSRTQLSYNSDFSG 1735

BLAST of Lag0040217.1 vs. NCBI nr
Match: XP_038893376.1 (protein PHYLLO, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 3109.3 bits (8060), Expect = 0.0e+00
Identity = 1541/1725 (89.33%), Postives = 1628/1725 (94.38%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M ADSL I KLA+ HPVRPFI  SFPRSKLFS+FA  PKG+ QP SFRLPQNSCFK VR+
Sbjct: 1    MQADSLRIHKLAVLHPVRPFITDSFPRSKLFSSFALIPKGSRQPASFRLPQNSCFKCVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GATELSDLEDV+ +VEMCIT T+PPALTLKQGLESISEAIEKLKLEPPRSS GVF
Sbjct: 61   DSPVMGATELSDLEDVEFLVEMCITHTLPPALTLKQGLESISEAIEKLKLEPPRSSHGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPSTRAL+WFCCQPESSEV+PIFFLS EKDATIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSTRALLWFCCQPESSEVYPIFFLSNEKDATIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            +LSSTSSK+STLKRYL+NDSAPIMAYGFVN NTGE SS+KN+A HS FC+PQIEL EYEG
Sbjct: 181  NLSSTSSKRSTLKRYLMNDSAPIMAYGFVNDNTGEISSLKNEAGHSCFCIPQIELSEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            ISVLSATLAWSESFPCTFEE +HSLG SI+QISTNFPS+  CQYKYIRSSLT+ KL+D T
Sbjct: 241  ISVLSATLAWSESFPCTFEEAIHSLGSSIYQISTNFPSKGNCQYKYIRSSLTASKLVDRT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEMESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEHT 360
            +QMAYMKVLSVAGESFRTGIMEMESA SHQFCIR+SP+VAVACNMLDHINE  HS QEH 
Sbjct: 301  NQMAYMKVLSVAGESFRTGIMEMESALSHQFCIRVSPNVAVACNMLDHINENVHSEQEHA 360

Query: 361  NISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG 420
            NI+A+WASLIVEECSRLGLTYFCIAPGSRSSPLA+AAASHPLITCIACFDERSLAFHAIG
Sbjct: 361  NINAVWASLIVEECSRLGLTYFCIAPGSRSSPLAVAAASHPLITCIACFDERSLAFHAIG 420

Query: 421  YAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQVN 480
            YAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQDAGANQAINQVN
Sbjct: 421  YAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQDAGANQAINQVN 480

Query: 481  HFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSPW 540
            HFGSFVRFFF+LP PTDQIPARMVLTTLDSAVHWATS+PCGPVHINCPFREPLENSPSPW
Sbjct: 481  HFGSFVRFFFSLPAPTDQIPARMVLTTLDSAVHWATSSPCGPVHINCPFREPLENSPSPW 540

Query: 541  NLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHSE 600
            NL+CLNGLHIWLSSTE FTKYI+LEASPTSIDTFG MAEVLK+IHGA+NGILLLGSI SE
Sbjct: 541  NLSCLNGLHIWLSSTEVFTKYIRLEASPTSIDTFGHMAEVLKVIHGAKNGILLLGSIQSE 600

Query: 601  DEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKWL 660
            DEIWAA LLAKHISWPIVADVLSGLRLRKSL+SFLE+E NFFFVDHLDHALLSD VRKWL
Sbjct: 601  DEIWAAFLLAKHISWPIVADVLSGLRLRKSLTSFLEMENNFFFVDHLDHALLSDLVRKWL 660

Query: 661  KFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGCL 720
            KFDVIIQIGSRVTSKR+SKMLEDC+PCSYIMVD++PGRHDPSHIVTHRIQSTVLEFVGCL
Sbjct: 661  KFDVIIQIGSRVTSKRISKMLEDCSPCSYIMVDKYPGRHDPSHIVTHRIQSTVLEFVGCL 720

Query: 721  LEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLGN 780
            L+ GFP +RS WT TL+ALNMMVE EIQFQISANYSLSEPQVAQ+ISEALP DSVLFLGN
Sbjct: 721  LKAGFPLSRSKWTTTLRALNMMVEWEIQFQISANYSLSEPQVAQVISEALPCDSVLFLGN 780

Query: 781  SMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVGC 840
            SMPIRDVDMYAYGWSKCNDSVAAR LNLQ+PF W WTSGNRGASGIDGLLSTAVGFSVGC
Sbjct: 781  SMPIRDVDMYAYGWSKCNDSVAARQLNLQLPFCWTWTSGNRGASGIDGLLSTAVGFSVGC 840

Query: 841  NKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAILD 900
            NKRVLC+VGD+SFLHDTNGLAILNQRM RKPVTVVVINNNGGAIFSLLPIKDKV+ AILD
Sbjct: 841  NKRVLCLVGDISFLHDTNGLAILNQRMARKPVTVVVINNNGGAIFSLLPIKDKVEAAILD 900

Query: 901  QYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTFH 960
            Q+FHTSH++SLRSLCMAHGLKHLHVRT MELQDALF S  EENDCIIEV+SSIDANTTFH
Sbjct: 901  QFFHTSHKVSLRSLCMAHGLKHLHVRTKMELQDALFQSHHEENDCIIEVDSSIDANTTFH 960

Query: 961  SVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSID 1020
            SVLRKFTCQAVDHGLR+FS L+ EESVS GLFLCKISRM+ TLFRIPLCAPPTT SSSID
Sbjct: 961  SVLRKFTCQAVDHGLRIFSGLHSEESVSSGLFLCKISRMECTLFRIPLCAPPTTASSSID 1020

Query: 1021 QVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSFI 1080
            QVSRKFFREGFILSL+LEDGSLGLGE+SPL+IHRE+LLDVEEQLKCL+HILKGAKI S I
Sbjct: 1021 QVSRKFFREGFILSLYLEDGSLGLGEISPLDIHRESLLDVEEQLKCLIHILKGAKIRSSI 1080

Query: 1081 PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENNL 1140
            PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLL+VLQ+QLDEE NL
Sbjct: 1081 PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLDVLQHQLDEEKNL 1140

Query: 1141 TSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKKL 1200
             +SSKVQICGLLDSGG+PS VA VAKTL EEGF  IKLKVARQGNVMYDAAVVQE+RKKL
Sbjct: 1141 ATSSKVQICGLLDSGGTPSEVAHVAKTLAEEGFPTIKLKVARQGNVMYDAAVVQEIRKKL 1200

Query: 1201 GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDET 1260
            GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAI+KFCEESGLPVALDET
Sbjct: 1201 GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIIKFCEESGLPVALDET 1260

Query: 1261 VDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETGV 1320
            +DHIQDNPVKELAKY HPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFE+GV
Sbjct: 1261 LDHIQDNPVKELAKYAHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFESGV 1320

Query: 1321 GLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSGI 1380
            GLSGYI LS YLEL NAE+RKLMNIQPAPSIAHGLGTYRWLEEDVT++PLRI RDP SGI
Sbjct: 1321 GLSGYIHLSCYLELQNAEVRKLMNIQPAPSIAHGLGTYRWLEEDVTLDPLRISRDPHSGI 1380

Query: 1381 IEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDNV 1440
            +EASVAEANRLLK FQINQKFVC+K TEEQV RY+L+VD KGFSY  KVLEVG RTNDNV
Sbjct: 1381 VEASVAEANRLLKGFQINQKFVCKKFTEEQVCRYKLSVDSKGFSYSTKVLEVGQRTNDNV 1440

Query: 1441 LVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEVV 1500
            L FLHGFLGTGEDWITIMK +SGSARCISLDLPGHG STTQKNDCDVH VEEPS+S+EV+
Sbjct: 1441 LFFLHGFLGTGEDWITIMKGISGSARCISLDLPGHGESTTQKNDCDVHGVEEPSYSIEVI 1500

Query: 1501 ANLLYKLIHHLAPGK--VNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR 1560
            A+LLYKLIHHLAPGK  VNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR
Sbjct: 1501 ADLLYKLIHHLAPGKGIVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR 1560

Query: 1561 IRRVKDDSRARILKVYGLQSFLEMWYCGELWK-SLREHPHYSEIIASRLKHDDVQHLAKA 1620
            IRRVKDDSRARILKVYGLQ FLE WYCGELWK SLREHPHYS+IIASRLKHDDVQHLAKA
Sbjct: 1561 IRRVKDDSRARILKVYGLQRFLEAWYCGELWKSSLREHPHYSQIIASRLKHDDVQHLAKA 1620

Query: 1621 LSDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELH 1680
            LSDLSIGRQPQLWDELK CK PLSIIVGEKDTKFKTIAQ+ILSQIDMG+KIKDEPVV+LH
Sbjct: 1621 LSDLSIGRQPQLWDELKCCKIPLSIIVGEKDTKFKTIAQQILSQIDMGKKIKDEPVVDLH 1680

Query: 1681 EIVEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSGLG 1723
            EIVEIPDSGHAAHLENPL VINALS+FLIRSRT+ SYN DF+G G
Sbjct: 1681 EIVEIPDSGHAAHLENPLDVINALSRFLIRSRTRCSYNPDFAGRG 1725

BLAST of Lag0040217.1 vs. ExPASy Swiss-Prot
Match: Q15KI9 (Protein PHYLLO, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PHYLLO PE=2 SV=2)

HSP 1 Score: 1866.3 bits (4833), Expect = 0.0e+00
Identity = 949/1658 (57.24%), Postives = 1224/1658 (73.82%), Query Frame = 0

Query: 56   KGVRFDSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRS 115
            +GV+FD P++      +L+D DLVV++C+TRT+PPALTL+ GLES+ EAI++LK  PP+S
Sbjct: 55   QGVQFDGPIMDRD--VNLDD-DLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKS 114

Query: 116  SSGVFRFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEK-DATIKSLYLNDTRGVFGIG 175
            SSGV RFQVAVPP  +AL WFC QP +S+VFP+FFLSK+  + + KSLY+ +  GVFGIG
Sbjct: 115  SSGVLRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLSKDTVEPSYKSLYVKEPHGVFGIG 174

Query: 176  TAIYFASLSSTSSK-QSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQI 235
             A  F   SS  S   S +K +L ++SA + AYGF +    + S++ +    SYF VPQI
Sbjct: 175  NAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSKDGSSYFFVPQI 234

Query: 236  ELCEYEGISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNF-PSREKCQYKYIRSSLT 295
            EL E+E +S+L+ TLAW+ES   T E+ + S   SI Q+S++F P+ E   +K+++SSL 
Sbjct: 235  ELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSHFCPNVEDHWFKHLKSSLA 294

Query: 296  SLKLMD-GTHQMAYMKVLSVAGESFRTGIMEMESAFSH-QFCIRLSPSVAVACNMLDHIN 355
             L + +    +M +M   + +G   +  + E++S  S  QF  +LSP V  + NML+   
Sbjct: 295  KLSVEEIHPLEMEHMGFFTFSGRD-QADVKELKSIQSSCQFHCKLSPDVVFSNNMLNRET 354

Query: 356  ETRHSMQEHTNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFD 415
            E  + +++  NI+A+WAS I+EEC+RLGLTYFC+APGSRSS LAIAAA+HPL TC+ACFD
Sbjct: 355  EVSNFLRDEANINAVWASAIIEECTRLGLTYFCVAPGSRSSHLAIAAANHPLTTCLACFD 414

Query: 416  ERSLAFHAIGYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDA 475
            ERSLAFHAIGYAKGS  PAVIITSSGTAVSNLLPAVVEAS+DF PLLLLTADRPPELQ  
Sbjct: 415  ERSLAFHAIGYAKGSLKPAVIITSSGTAVSNLLPAVVEASEDFLPLLLLTADRPPELQGV 474

Query: 476  GANQAINQVNHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFR 535
            GANQAINQ+NHFGSFVRFFFNLP PTD IP RMVLTT+DSA+HWAT + CGPVH+NCPFR
Sbjct: 475  GANQAINQINHFGSFVRFFFNLPPPTDLIPVRMVLTTVDSALHWATGSACGPVHLNCPFR 534

Query: 536  EPLENSPSPWNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNG 595
            +PL+ SP+ W+ NCLNGL +W+S+ E FTKY Q+++  +   T G++ E+L++I  A+ G
Sbjct: 535  DPLDGSPTNWSSNCLNGLDMWMSNAEPFTKYFQVQSHKSDGVTTGQITEILQVIKEAKKG 594

Query: 596  ILLLGSIHSEDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHA 655
            +LL+G+IH+EDEIWA++LLAK + WP+VADVLSG+RLRK    F+E +    FVDHLDHA
Sbjct: 595  LLLIGAIHTEDEIWASLLLAKELMWPVVADVLSGVRLRKLFKPFVE-KLTHVFVDHLDHA 654

Query: 656  LLSDSVRKWLKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQ 715
            L SDSVR  ++FDV+IQ+GSR+TSKRVS+MLE C P +YI+VD+HP RHDPSH+VTHR+Q
Sbjct: 655  LFSDSVRNLIEFDVVIQVGSRITSKRVSQMLEKCFPFAYILVDKHPCRHDPSHLVTHRVQ 714

Query: 716  STVLEFVGCLLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEAL 775
            S +++F  C+L+  FP  RS     LQAL+  +  E+ FQISA  SL+EP VA ++S+AL
Sbjct: 715  SNIVQFANCVLKSRFPWRRSKLHGHLQALDGAIAREMSFQISAESSLTEPYVAHMLSKAL 774

Query: 776  PSDSVLFLGNSMPIRDVDMYAYGWSKCNDS-VAARPLNLQVPFFWKWTSGNRGASGIDGL 835
             S S LF+GNSMPIRDVDM  YG S  N S V    L+ ++P  W   +GNRGASGIDGL
Sbjct: 775  TSKSALFIGNSMPIRDVDM--YGCSSENSSHVVDMMLSAELPCQWIQVTGNRGASGIDGL 834

Query: 836  LSTAVGFSVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLP 895
            LS+A GF+VGC KRV+CVVGD+SFLHDTNGLAIL QR+ RKP+T++VINN GG IF LLP
Sbjct: 835  LSSATGFAVGCKKRVVCVVGDISFLHDTNGLAILKQRIARKPMTILVINNRGGGIFRLLP 894

Query: 896  IKDKVDPAILDQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEV 955
            I  K +P++L+QYF+T+H IS+ +LC+AHG++++HV T  EL+DALF+   EE DCI+EV
Sbjct: 895  IAKKTEPSVLNQYFYTAHDISIENLCLAHGVRYVHVGTKSELEDALFVPSVEEMDCIVEV 954

Query: 956  ESSIDANTTFHSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLC 1015
            ESSI+AN   HS L +F  QA ++ L + S   F   +   + LC++S + Y+ +R+ LC
Sbjct: 955  ESSINANAIVHSTLERFARQAAENSLGIVSASSFLHPMIKNVLLCQVSGIQYSQYRVKLC 1014

Query: 1016 APPTTTSSSIDQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLH 1075
              PT  S    Q    F REGFILSL LEDGS+G GEV+PL  + ENL+DVE QL+ +LH
Sbjct: 1015 DRPTICSDEFSQ----FHREGFILSLTLEDGSIGYGEVAPLNSNVENLMDVEGQLQLVLH 1074

Query: 1076 ILKGAKISSFIPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNV 1135
            ++  AK S  +PLL GS SSWI+ ELGI  SSI+PSVRCGLEMA+LNA+A R   SLL +
Sbjct: 1075 LMNEAKFSYMLPLLNGSISSWIWSELGITASSIFPSVRCGLEMALLNAMAVRHDSSLLGI 1134

Query: 1136 LQYQLDEENNLTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYD 1195
            L YQ  EEN       VQIC LLDS G+P  VA VA+ LV+EGF+AIKLKV R+ + + D
Sbjct: 1135 LHYQ-KEENGSAQPHSVQICALLDSEGTPLEVAYVARKLVQEGFSAIKLKVGRRVSSVQD 1194

Query: 1196 AAVVQEVRKKLGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCE 1255
            A V+QEVR+ +G QIELR DAN  W++EEA  F  LV  C L+YIEEPV ++D +++F E
Sbjct: 1195 ALVMQEVRRAVGVQIELRADANCRWTFEEAREFGLLVNSCNLKYIEEPVQNKDDLIRFHE 1254

Query: 1256 ESGLPVALDETVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKM 1315
            E+GLPVALDET+D  ++ P++ L KY HPGIVA+VIKPSV+GGFENAAL+ARWAQQHGKM
Sbjct: 1255 ETGLPVALDETLDDFEECPLRMLTKYTHPGIVAVVIKPSVVGGFENAALIARWAQQHGKM 1314

Query: 1316 AVVSAAFETGVGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNP 1375
            AV+SAA+E+G+GLS YI  +SYLE+ N +          PS+AHGLGTYRWL EDV +N 
Sbjct: 1315 AVISAAYESGLGLSAYILFASYLEMENVKASTEQKQGTPPSVAHGLGTYRWLSEDVMMNT 1374

Query: 1376 LRICRDPCSGIIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKV 1435
            L I R P SG +E  +A+A+R LK+ +IN   + R S    VRRY+L VD+ GFS+ I+V
Sbjct: 1375 LGIFRSPYSGFVEGFIADASRNLKDVKINNDVIVRTSKGIPVRRYELRVDVDGFSHFIRV 1434

Query: 1436 LEVGPRTNDNVLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHD 1495
             +VG     +V +FLHGFLGTGE+WI IM  +SGSARCIS+D+PGHG S  Q +  +   
Sbjct: 1435 HDVGENAEGSVALFLHGFLGTGEEWIPIMTGISGSARCISVDIPGHGRSRVQSHASETQ- 1494

Query: 1496 VEEPSFSMEVVANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSP 1555
               P+FSME++A  LYKLI  + PGKV +VGYSMGARIA+YMALRF +KI  AV++SGSP
Sbjct: 1495 -TSPTFSMEMIAEALYKLIEQITPGKVTIVGYSMGARIALYMALRFSNKIEGAVVVSGSP 1554

Query: 1556 GLKDKMARRIRRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHD 1615
            GLKD +AR+IR   DDS+AR++   GL  F+E WY G LWKSLR HPH+S+I ASRL H 
Sbjct: 1555 GLKDPVARKIRSATDDSKARMMVDNGLYIFIENWYNGGLWKSLRNHPHFSKIAASRLLHG 1614

Query: 1616 DVQHLAKALSDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIK 1675
            DV  +AK LSDLS GRQP LW+EL+ C T +S++ GEKD K+K IA ++  ++   +K  
Sbjct: 1615 DVPSVAKLLSDLSSGRQPSLWEELEDCDTNISLVFGEKDVKYKQIATRMYREMSKSKK-- 1674

Query: 1676 DEPVVELHEIVEIPDSGHAAHLENPLAVINALSKFLIR 1708
               V  + EIVEIP++GHA HLE+PL VI AL KFL R
Sbjct: 1675 --SVNNIIEIVEIPEAGHAVHLESPLRVILALRKFLTR 1694

BLAST of Lag0040217.1 vs. ExPASy Swiss-Prot
Match: B4S4J4 (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase OS=Prosthecochloris aestuarii (strain DSM 271 / SK 413) OX=290512 GN=menD PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-100
Identity = 217/614 (35.34%), Postives = 330/614 (53.75%), Query Frame = 0

Query: 359 HTNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHA 418
           H  I+ LW+ +IVEE  R  + +FCI+PGSRS+PL +AA+ H   TC    DER+ AF A
Sbjct: 3   HKEITTLWSWIIVEELVRNSICFFCISPGSRSTPLTVAASRHQKTTCKIFPDERAAAFFA 62

Query: 419 IGYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQ 478
           +GYA+ +  PAV+I +SGTA +N  PAVVEAS    P+L+L+ADRP EL++ GANQ I Q
Sbjct: 63  LGYARATGRPAVLICTSGTAAANYFPAVVEASMGHQPMLVLSADRPFELRETGANQTIRQ 122

Query: 479 VNHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPS 538
              +GS+ R+ F LP P+   P   +L+ +D AV   T+ P GPVH+N  FREPLE  P 
Sbjct: 123 SGIYGSYSRWSFQLPEPSTDTPPEAILSAIDYAVSTCTANPSGPVHLNIAFREPLEPVPL 182

Query: 539 PWNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIH 598
             N   L+ L  W SS   +++ +Q ++SP S      + EV +++  A N +++ G + 
Sbjct: 183 NENSPWLSSLGKWNSSRAPWSRTLQRQSSPES----ASVKEVARLLASAENPLIIAGHLD 242

Query: 599 SEDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRK 658
              +  A + L+K ++  + AD+ S LRL K   +             L  A LSD   +
Sbjct: 243 RPADAQAVLNLSKSLNIALYADISSQLRLHKETVA-------------LQQAWLSDKYVE 302

Query: 659 WLKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVG 718
             + D+++  G  +  K+  + ++   P   I++  HP R+ P H VT  I+++V  F  
Sbjct: 303 QHRADLVLHFGGSLVGKKPGQAMKTWRPDHTIVIKNHPDRYAPDHTVTMSIEASVKAFAE 362

Query: 719 CLLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFL 778
            L +   P+ +             +E EI+     +  ++E   A+I+S  +     LFL
Sbjct: 363 ALAKTSQPQGKKANPID------EIEQEIERFTRPDSPVTEISAARIVSRLIDPGHGLFL 422

Query: 779 GNSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSV 838
            NSMP+RD+DMY           A R     +P     T+ NRGASGIDG++S+A GF+ 
Sbjct: 423 ANSMPVRDMDMY-----------ATRSGGTIIP-----TAMNRGASGIDGIISSAAGFAS 482

Query: 839 GCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAI 898
           G  + V  ++GD+SFLHD N L +L  R    P+T+VVINNNGG IFS LPI D+  P +
Sbjct: 483 GLERPVTLLIGDISFLHDMNALCLL--RSMTVPLTIVVINNNGGGIFSFLPISDQ--PDV 542

Query: 899 LDQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTT 958
            ++ F T    ++ +   A  +++    +N    ++   ++      IIE+ S  D N  
Sbjct: 543 FEKNFGTPQEFNIAAAATAFSIEYQCPPSNAAFSESYMAARSSAETSIIEIRSRRDENLA 573

Query: 959 FHSVLRKFTCQAVD 973
            H  L +     +D
Sbjct: 603 LHRKLNQSLIDRLD 573

BLAST of Lag0040217.1 vs. ExPASy Swiss-Prot
Match: Q2S2V6 (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase OS=Salinibacter ruber (strain DSM 13855 / M31) OX=309807 GN=menD PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 2.0e-96
Identity = 225/625 (36.00%), Postives = 326/625 (52.16%), Query Frame = 0

Query: 355 SMQEHTNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSL 414
           S+ +  N + LW  L+VEE  R G+  F +APGSRS+PL +A A HP    +   DER  
Sbjct: 7   SVLDAPNPTYLWTQLLVEELVRNGVHTFFVAPGSRSTPLTVAIARHPEAESVLHVDERGA 66

Query: 415 AFHAIGYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQ 474
           AF A+G  + +  PA  +T+SGTAV+N LPA VEAS D  P+LLLTADRPPEL+D GANQ
Sbjct: 67  AFAALGVGRAARGPAAWVTTSGTAVANGLPAAVEASVDGVPMLLLTADRPPELRDTGANQ 126

Query: 475 AINQVNHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLE 534
            I+QV  FG +VR+  ++P P+D++    VLTT D A+H    AP GPVH+NC FR+PLE
Sbjct: 127 TIDQVKIFGDYVRWQADVPPPSDEVDPAYVLTTADQALHQTLRAPAGPVHVNCMFRKPLE 186

Query: 535 NSPSPWNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLL 594
              +  ++     +  W   TE +T Y     SP   +    +  + + + G  +G+++ 
Sbjct: 187 PVETEASVAVPTAVDAWARGTEPYTHYPTPAPSPPGPE----VDALAETVRGTEHGLVVA 246

Query: 595 GSIHSEDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSD 654
           G + S     A   LA H+ WP++ D+ S  RLR+      E       V + D  L S 
Sbjct: 247 GRLDSAAAADATRRLATHLGWPLIPDLTS--RLRRGGREQPE------QVPYGDLVLTSA 306

Query: 655 SVRKWLKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVL 714
           + R+      ++Q+G R  SKR+   L D  P  + +V   P R DP H VTH +++ V 
Sbjct: 307 AFREGHPPRAVLQVGGRFASKRLRLFLRDSAPEVWAVVRPDPSRIDPDHRVTHHVEAAVP 366

Query: 715 EFVGCL---LEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALP 774
             V  L   LE G PR  +TW       +  V + +Q  +  + +L++P VA +++E +P
Sbjct: 367 AAVDALVARLEEG-PRG-TTWRDDWAGASERVGAVVQAHVQESDALTDPLVAALLTEEMP 426

Query: 775 SDSVLFLGNSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLS 834
           S+  L   +SMP+RD++ +            A P     P F      NRGASGIDG ++
Sbjct: 427 SEHALVAASSMPVRDLNRH------------AAPGGTGGPAF-----ANRGASGIDGTVA 486

Query: 835 TAVGFSVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIK 894
           TA G + G +  V  ++GD++  HD NGLA+L  R    PV  +V+NN+GG IF  LPI+
Sbjct: 487 TAAGIAEGRDGPVTLLIGDLALQHDLNGLALLQDR----PVVAIVVNNDGGGIFHFLPIR 546

Query: 895 --DKVDPAILDQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEV 954
             D+ DP     YF T H            L +    +   L+ A   + R     +IEV
Sbjct: 547 KHDEFDP-----YFTTPHGHDFEHAAALFDLPYHRPDSPSALRSAYAQACRSGESALIEV 591

Query: 955 ESSIDANTTFHSVLRKFTCQAVDHG 975
            +    N   H  L     +AV+ G
Sbjct: 607 RTDRATNRQVHDRLEASVERAVEEG 591

BLAST of Lag0040217.1 vs. ExPASy Swiss-Prot
Match: B3QL00 (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=menD PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 7.6e-96
Identity = 213/610 (34.92%), Postives = 319/610 (52.30%), Query Frame = 0

Query: 359 HTNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHA 418
           H  I+ LW  +IVEE  R G  +FCI+PGSRS+PL +A A++P        DERS  F+A
Sbjct: 3   HKQITTLWCRVIVEELIRQGAGFFCISPGSRSTPLTLAVAANPNARFRMFPDERSAGFYA 62

Query: 419 IGYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQ 478
           +GYA+ +  PAV++ +SGTAV+N  PAVVEAS D  P+L+L+ADRP EL DAGANQ I Q
Sbjct: 63  LGYARAAGKPAVLVCTSGTAVANYFPAVVEASADAQPMLVLSADRPFELLDAGANQTIRQ 122

Query: 479 VNHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLE---- 538
            + FGS+ R+   LP P    P   +L+T+  AV  +  +P GPVH+N PFREPLE    
Sbjct: 123 QDIFGSYTRWNLELPEPGTGTPLASLLSTVGQAVKRSLGSPAGPVHLNLPFREPLEPESH 182

Query: 539 NSPSPWNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLL 598
           +   PW    +  L  WL+S + + ++ Q   +P +      +  + +I+  A   + + 
Sbjct: 183 DLAHPW----VEPLRNWLASEQPWCRFEQPRTAPDA----NALTALQQILANAERPLFVA 242

Query: 599 GSIHSEDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSD 658
           GS+   D+  A   LA  +   + AD+ SGLRL    + +               A  ++
Sbjct: 243 GSMDKADDAEAVASLADSLGILLFADLTSGLRLTNKCTPW-------------QLAFQNE 302

Query: 659 SVRKWLKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVL 718
           +     K DV+I  G  +  K+ +  L    P  Y++V  HPGR +P H VT  I+++  
Sbjct: 303 TFATSFKPDVVIHFGGALIGKQPAMTLRKEPPFHYVVVRNHPGRFNPDHNVTLSIEASPA 362

Query: 719 EFVGCLLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDS 778
             V  L E   P     + A    +   +++      + +  ++E    +I+S       
Sbjct: 363 AVVSALSECRKPAEMGGFAALFSEVAHTIDTA---ACAPDEPVNEISAPRIVSSLTDGKH 422

Query: 779 VLFLGNSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAV 838
            LF+ NSMP RD+D+YA         V+ +PL +++         NRG SGIDG++STA 
Sbjct: 423 ALFVANSMPARDMDLYA-------APVSEKPLRVEL---------NRGVSGIDGIISTAA 482

Query: 839 GFSVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKV 898
           GFS G +K    ++GD+SFLHD N L++L+      P+ V+V+NNNGG IFS LPI  + 
Sbjct: 483 GFSAGLDKPTTLLIGDISFLHDLNALSLLDN--PTNPLIVIVLNNNGGGIFSFLPIASQT 542

Query: 899 DPAILDQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSID 958
           D   LD+ F T    S+ S      L +    +N E  +    + +     +IE+ S+  
Sbjct: 543 DR--LDECFATPQNFSIESAARTFDLDYASPASNREFTELYADALKRNKSLVIEIRSNRQ 568

Query: 959 ANTTFHSVLR 965
           AN   H  L+
Sbjct: 603 ANLLLHRTLK 568

BLAST of Lag0040217.1 vs. ExPASy Swiss-Prot
Match: Q8KBE8 (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=menD PE=3 SV=2)

HSP 1 Score: 351.7 bits (901), Expect = 4.9e-95
Identity = 220/608 (36.18%), Postives = 319/608 (52.47%), Query Frame = 0

Query: 362 ISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIGY 421
           I+ LW ++IVEE  R    +FCI+PGSRS+PL +A AS+P        DERS  F+A+GY
Sbjct: 6   ITTLWCAVIVEELIRQEAGFFCISPGSRSTPLTLAVASNPKARFRMFPDERSAGFYALGY 65

Query: 422 AKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQVNH 481
           A+ +  PAV++ +SGTAV+N  PAVVEAS D  P+L+L+ADRP EL + GANQAI Q N 
Sbjct: 66  ARATGMPAVLVCTSGTAVANYFPAVVEASADAQPMLVLSADRPFELLECGANQAIRQQNI 125

Query: 482 FGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLE-NSPSPW 541
           FGS+ R+ F LP P    P   +L+T+D AV  + S P GPVH+N PFREPLE  +P P 
Sbjct: 126 FGSYTRWSFELPEPGIATPLASLLSTVDHAVRKSLSLPAGPVHLNLPFREPLEPEAPDPG 185

Query: 542 NLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHSE 601
           +      L  W +S E ++++    A P    +   +  + +++  A   + + GS+ + 
Sbjct: 186 H-PWAAPLETWQASGEPWSRF----ARPLHEPSAESIVTLRELLAQAERPLFVAGSMSNA 245

Query: 602 DEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKWL 661
            +  A   LA+ +  P+ AD+ SG+RL    + +    +N  FV+               
Sbjct: 246 ADGEAVAALAESLGVPLFADLTSGIRLSSDCTPWQLAFQNEAFVERFQP----------- 305

Query: 662 KFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQST----VLEF 721
             DV+I  G  V  K+ +  L    P  Y++V EHPGR DP H VT  ++++        
Sbjct: 306 --DVVIHFGGHVIGKQPAMALRKQPPLHYVVVREHPGRFDPDHNVTLTLEASPAAVASAL 365

Query: 722 VGCLLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVL 781
            GC   V   R R  ++A    ++ M             ++SE    +I+S        L
Sbjct: 366 EGCREPVPGIRCRDAFSAASGIIDKMA-------CVPELAVSEISAPRIVSSLAGDGHAL 425

Query: 782 FLGNSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGF 841
           F+ NSMP RD+D+YA         VA +PL + +         NRG SGIDG++STA GF
Sbjct: 426 FVANSMPARDMDLYA-------APVAQKPLQVAL---------NRGVSGIDGIISTAAGF 485

Query: 842 SVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDP 901
           S G  K    ++GD+SFLHD N L +LN      P+ V+V+NN+GG+IFS LPI  + D 
Sbjct: 486 SAGLGKPTTLLIGDISFLHDLNALCLLNHPW--NPLIVIVLNNHGGSIFSFLPIASQTDR 545

Query: 902 AILDQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDAN 961
             LD+ F T    S+ S     G+ +    TN +       +   +   IIE+ S  + N
Sbjct: 546 --LDECFATPQNFSIESAARTFGIDYACPETNGDFTQLYAEALTTKKSLIIEIRSDREKN 568

Query: 962 TTFHSVLR 965
              H  L+
Sbjct: 606 LLLHRSLK 568

BLAST of Lag0040217.1 vs. ExPASy TrEMBL
Match: A0A6J1GXJ7 (protein PHYLLO, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458428 PE=3 SV=1)

HSP 1 Score: 3119.7 bits (8087), Expect = 0.0e+00
Identity = 1559/1721 (90.59%), Postives = 1628/1721 (94.60%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+LSI   ALPHPVRPF R SFPRS LFSTF F PK +  PGSFRLPQ SCFKGVR+
Sbjct: 1    MKADTLSIHNPALPHPVRPFNRASFPRSNLFSTFGFIPKPSRHPGSFRLPQTSCFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GATELS+LEDV+L+VEMCIT T+PPALTLKQGLESISEAI+KLKLE PRSS GVF
Sbjct: 61   DSPVMGATELSELEDVELMVEMCITHTLPPALTLKQGLESISEAIDKLKLESPRSSDGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS RALMWFCCQPESSE++PIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSERALMWFCCQPESSEMYPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQ TLKRYL+NDSAPIMAYGFVNANTGETSS+KN+A HSYFCVPQIEL EYEG
Sbjct: 181  SLSSTSSKQCTLKRYLMNDSAPIMAYGFVNANTGETSSLKNEAGHSYFCVPQIELNEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            +SVLSATLAWSE+FPCTFEE LHSLG SI+QI TNFPS+E  QYKYIRSSLT+ KL+D T
Sbjct: 241  VSVLSATLAWSEAFPCTFEEALHSLGSSIYQIGTNFPSKENFQYKYIRSSLTASKLVDVT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEM-ESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEH 360
            HQM YMKV SVAGESFRTGIMEM ESA SHQFCIRLSPSVAVACNMLD  NET  S QEH
Sbjct: 301  HQMGYMKVFSVAGESFRTGIMEMKESARSHQFCIRLSPSVAVACNMLDRTNETCLSEQEH 360

Query: 361  TNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAI 420
             NI+ALWAS IVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCI+CFDERSLAFHAI
Sbjct: 361  ANINALWASFIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCISCFDERSLAFHAI 420

Query: 421  GYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQV 480
            GYAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQDAGANQAINQV
Sbjct: 421  GYAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQDAGANQAINQV 480

Query: 481  NHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSP 540
            NHFGSFVRFFF+LP PTD+IPARMVLTTLDSAVHWATS+PCGPVHINCPFREPLENSPSP
Sbjct: 481  NHFGSFVRFFFSLPAPTDEIPARMVLTTLDSAVHWATSSPCGPVHINCPFREPLENSPSP 540

Query: 541  WNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHS 600
            WNL+ LNGL  WLSS+EAFTKYI+LEASP SIDTFG MAEVLK+IHGARNGILLLGSI S
Sbjct: 541  WNLSSLNGLQNWLSSSEAFTKYIRLEASPMSIDTFGHMAEVLKVIHGARNGILLLGSIQS 600

Query: 601  EDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKW 660
            EDEIWAA LLAKHISWPIVADVLSGLRLRKSLSSFLE+E NFFFVDHLDHALLSDSVRKW
Sbjct: 601  EDEIWAAFLLAKHISWPIVADVLSGLRLRKSLSSFLEVENNFFFVDHLDHALLSDSVRKW 660

Query: 661  LKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720
            LKFDVIIQ+GSRVTSKRVSKMLEDC+PCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC
Sbjct: 661  LKFDVIIQMGSRVTSKRVSKMLEDCSPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720

Query: 721  LLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLG 780
            LL+ GFPRNRS WTATL ALNMMVE EIQFQISANY LSEPQVAQ+ISEALP DSVLFLG
Sbjct: 721  LLKTGFPRNRSKWTATLLALNMMVEWEIQFQISANYCLSEPQVAQVISEALPFDSVLFLG 780

Query: 781  NSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVG 840
            NSMPIRDVDMYA GWSKC DSVA RPLNLQ+PF W WTSGNRGASGIDGLLSTAVGFSVG
Sbjct: 781  NSMPIRDVDMYARGWSKCTDSVAVRPLNLQLPFCWTWTSGNRGASGIDGLLSTAVGFSVG 840

Query: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAIL 900
            CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKV+ AIL
Sbjct: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVEAAIL 900

Query: 901  DQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTF 960
            DQ+FHTSH+ISLRSLC+AHGLKHLHVRT MELQDALFMSQ EENDCIIEVESSIDANT+F
Sbjct: 901  DQFFHTSHKISLRSLCVAHGLKHLHVRTKMELQDALFMSQCEENDCIIEVESSIDANTSF 960

Query: 961  HSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSI 1020
            HSVLRKFTCQAVDHGLR+FSRLYFEESVSPGLFLCKISRM+YTLFRIPLCAP TT+SSSI
Sbjct: 961  HSVLRKFTCQAVDHGLRIFSRLYFEESVSPGLFLCKISRMEYTLFRIPLCAPTTTSSSSI 1020

Query: 1021 DQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSF 1080
            DQV+R FFREGFILSLFLEDGSLGLGEVSPLEIH+ENLLDVEEQLKCLLHI KGAKISS 
Sbjct: 1021 DQVNRGFFREGFILSLFLEDGSLGLGEVSPLEIHKENLLDVEEQLKCLLHIFKGAKISSS 1080

Query: 1081 IPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENN 1140
            IPLLRGSFSSWIFHELGI P SIYPSVRCGLEMAVLNAIAGRKGCSLL+VLQ++L EE++
Sbjct: 1081 IPLLRGSFSSWIFHELGIHPRSIYPSVRCGLEMAVLNAIAGRKGCSLLDVLQHRLVEEDD 1140

Query: 1141 LTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKK 1200
            L+SSSKV+ICGLLDSGG+PS VARVAKTLVEEGF AIKLKVARQGNVMYDAAVVQEVRKK
Sbjct: 1141 LSSSSKVKICGLLDSGGTPSEVARVAKTLVEEGFPAIKLKVARQGNVMYDAAVVQEVRKK 1200

Query: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260
            LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE
Sbjct: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260

Query: 1261 TVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETG 1320
            T+D IQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFE+G
Sbjct: 1261 TIDGIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFESG 1320

Query: 1321 VGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSG 1380
            VGLSGYI LS YLEL NAE+RKLM+IQPAPSIAHGLGT+RWL+EDVTVNPLRI RDP SG
Sbjct: 1321 VGLSGYIHLSCYLELQNAEIRKLMDIQPAPSIAHGLGTFRWLDEDVTVNPLRIGRDPHSG 1380

Query: 1381 IIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDN 1440
            IIEASVAEANRLLK+FQINQK VCRK TEEQVRRY+LTVD KGFSYLIKVLEVG RTNDN
Sbjct: 1381 IIEASVAEANRLLKSFQINQKVVCRKFTEEQVRRYKLTVDSKGFSYLIKVLEVGQRTNDN 1440

Query: 1441 VLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEV 1500
            VL+FLHGFLGTGEDWITIMKA+SGSARCIS+DLPGHG STTQKNDCDVH VEEPSFSMEV
Sbjct: 1441 VLLFLHGFLGTGEDWITIMKAISGSARCISIDLPGHGESTTQKNDCDVHGVEEPSFSMEV 1500

Query: 1501 VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARRI 1560
            VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRF DKIGRA IISGSPGLKDKMARRI
Sbjct: 1501 VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFADKIGRAAIISGSPGLKDKMARRI 1560

Query: 1561 RRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKALS 1620
            RRVKDDSRARILK YGL+SFLE WY GELWKSLREHPHYS+IIASR KHDDVQHLAKALS
Sbjct: 1561 RRVKDDSRARILKAYGLESFLEAWYRGELWKSLREHPHYSQIIASRSKHDDVQHLAKALS 1620

Query: 1621 DLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELHEI 1680
            DLSIGRQPQLWDELK CKTPLSIIVGEKDTKFKTIAQ+ILSQIDMGRKIKD+PVV LHEI
Sbjct: 1621 DLSIGRQPQLWDELKCCKTPLSIIVGEKDTKFKTIAQQILSQIDMGRKIKDDPVVSLHEI 1680

Query: 1681 VEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            VEIPDSGHAAHLENPLAVINALSKFLIRSRTQ SYNSDFSG
Sbjct: 1681 VEIPDSGHAAHLENPLAVINALSKFLIRSRTQLSYNSDFSG 1721

BLAST of Lag0040217.1 vs. ExPASy TrEMBL
Match: A0A6J1JTD3 (protein PHYLLO, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488697 PE=3 SV=1)

HSP 1 Score: 3104.3 bits (8047), Expect = 0.0e+00
Identity = 1552/1721 (90.18%), Postives = 1630/1721 (94.71%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+LSI K ALPHPVRPF R SFPRS LFSTF F PK +  PGSFRLPQ SCFKGVR+
Sbjct: 1    MKADTLSIHKPALPHPVRPFNRASFPRSILFSTFGFIPKPSRHPGSFRLPQTSCFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GATELS+LEDV+L+VEMCIT T+PP+LTLKQGLESISEAIEKLKLE PRSS GVF
Sbjct: 61   DSPVMGATELSELEDVELMVEMCITHTLPPSLTLKQGLESISEAIEKLKLESPRSSDGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS RALMWFCCQPESSEV+PIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSARALMWFCCQPESSEVYPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQ TLKRYL+NDSAPIMAYGFVNANTGETSS+KN+A HSYFCVPQIEL EYEG
Sbjct: 181  SLSSTSSKQCTLKRYLMNDSAPIMAYGFVNANTGETSSLKNEAGHSYFCVPQIELNEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            +SVLSATLAWSE+FPCTFEE LHSLG SI+QI TNFPSRE  QYKYIRSSLT+   +D T
Sbjct: 241  VSVLSATLAWSEAFPCTFEEALHSLGSSIYQIGTNFPSRENFQYKYIRSSLTASNHVDVT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEM-ESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEH 360
            +QM YMKV SVAGESFRTGIMEM ESA SHQFCIRLSPSVAVACNMLD  NET  S QEH
Sbjct: 301  NQMGYMKVFSVAGESFRTGIMEMKESARSHQFCIRLSPSVAVACNMLDRTNETCLSEQEH 360

Query: 361  TNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAI 420
             NI+ALWAS IVEECSRLGLTYFCIAPGSRSS LAIAAASHPLITCI+CFDERSLAFHAI
Sbjct: 361  ANINALWASFIVEECSRLGLTYFCIAPGSRSSLLAIAAASHPLITCISCFDERSLAFHAI 420

Query: 421  GYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQV 480
            GYAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQ+AGANQAINQV
Sbjct: 421  GYAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQEAGANQAINQV 480

Query: 481  NHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSP 540
            NHFGSFVRFFF+LP PTD+IPARMVLTTLDSAV+WATS+PCGPVHINCPFREPLENS SP
Sbjct: 481  NHFGSFVRFFFSLPAPTDEIPARMVLTTLDSAVNWATSSPCGPVHINCPFREPLENS-SP 540

Query: 541  WNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHS 600
            WNL+ LNGL  WLSS+EAFTKYI+LEASP SIDTFG MAEVLK+IHGARNGILLLGSI S
Sbjct: 541  WNLSSLNGLQSWLSSSEAFTKYIRLEASPMSIDTFGHMAEVLKVIHGARNGILLLGSIQS 600

Query: 601  EDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKW 660
            EDEIWAA LLAKHISWPIVADVLSGLRLRKSLSSFLE+E NFFFVDHLDHALLSDSVRKW
Sbjct: 601  EDEIWAAFLLAKHISWPIVADVLSGLRLRKSLSSFLEVENNFFFVDHLDHALLSDSVRKW 660

Query: 661  LKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720
            LKFDVIIQ+GSRVTSKRVSKMLEDC+PCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC
Sbjct: 661  LKFDVIIQMGSRVTSKRVSKMLEDCSPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720

Query: 721  LLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLG 780
            LL+ GFPRNRS WTATLQALNMMVE EIQFQISANY LSEPQVAQ+ISEALP DSVLFLG
Sbjct: 721  LLKTGFPRNRSKWTATLQALNMMVEWEIQFQISANYCLSEPQVAQVISEALPFDSVLFLG 780

Query: 781  NSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVG 840
            NSMPIRDVDMYA GWSKC DSVAARPLNLQ+PF W WTSGNRGASGIDGLLSTAVGFSVG
Sbjct: 781  NSMPIRDVDMYARGWSKCTDSVAARPLNLQLPFCWTWTSGNRGASGIDGLLSTAVGFSVG 840

Query: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAIL 900
            CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKV+ AIL
Sbjct: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVEAAIL 900

Query: 901  DQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTF 960
            DQ+FHTSH+ISLRSLC+AHGLKHLHVRT MELQDALFMSQ EENDCIIEVESSIDANT+F
Sbjct: 901  DQFFHTSHKISLRSLCVAHGLKHLHVRTKMELQDALFMSQCEENDCIIEVESSIDANTSF 960

Query: 961  HSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSI 1020
            HSVLRKFTCQAVDHGLR+FSRLYFEESVSPGLFLCKISRM+YTLFRIPLCAPPTT+SSSI
Sbjct: 961  HSVLRKFTCQAVDHGLRIFSRLYFEESVSPGLFLCKISRMEYTLFRIPLCAPPTTSSSSI 1020

Query: 1021 DQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSF 1080
            DQ++R FFREGFILSLFLEDGSLGLGEVSPLEIH+ENLLDVEEQLKCLLHILKGAKISS 
Sbjct: 1021 DQINRGFFREGFILSLFLEDGSLGLGEVSPLEIHKENLLDVEEQLKCLLHILKGAKISSS 1080

Query: 1081 IPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENN 1140
            IPLLRGSFSSWIFHELGIPP SIYPSVRCGLEMA+LNAIAGRKGCSLL+VLQ++L EE++
Sbjct: 1081 IPLLRGSFSSWIFHELGIPPRSIYPSVRCGLEMAILNAIAGRKGCSLLDVLQHRLVEEDD 1140

Query: 1141 LTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKK 1200
            L+SSSKV+ICGLLDSGG+PS VARVAKTLV+EGF AIKLKVARQGNVMYDAAVVQEVRKK
Sbjct: 1141 LSSSSKVKICGLLDSGGTPSEVARVAKTLVKEGFPAIKLKVARQGNVMYDAAVVQEVRKK 1200

Query: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260
            LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFC+ESGLPVALDE
Sbjct: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCKESGLPVALDE 1260

Query: 1261 TVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETG 1320
            T+D IQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVS+AFE+G
Sbjct: 1261 TIDGIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSSAFESG 1320

Query: 1321 VGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSG 1380
            VGLSGYI LS YLEL NAE+RKLM+IQPAPSIAHGLGT+RWL+EDVTVNPLRI RDP SG
Sbjct: 1321 VGLSGYIHLSCYLELQNAEIRKLMDIQPAPSIAHGLGTFRWLDEDVTVNPLRIGRDPHSG 1380

Query: 1381 IIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDN 1440
            IIEAS AEANRLLK+FQINQKFV RK TEEQVRRY+LTVD KGFSYLIKVLEVG RTNDN
Sbjct: 1381 IIEASAAEANRLLKSFQINQKFVRRKFTEEQVRRYKLTVDSKGFSYLIKVLEVGQRTNDN 1440

Query: 1441 VLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEV 1500
            VL+FLHGFLGTGEDWITIMKA+SGSARCIS+DLPGHG STTQKNDCDVH VEEP+FSMEV
Sbjct: 1441 VLLFLHGFLGTGEDWITIMKAISGSARCISIDLPGHGESTTQKNDCDVHGVEEPNFSMEV 1500

Query: 1501 VANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARRI 1560
            VANLLYKLIHHLAPGKVNLVGYSMG+RIAMYMALRF DKIGRAVIISGSPGLKDKMARRI
Sbjct: 1501 VANLLYKLIHHLAPGKVNLVGYSMGSRIAMYMALRFADKIGRAVIISGSPGLKDKMARRI 1560

Query: 1561 RRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKALS 1620
            RRVKDDSRARILK YGL+SFLE WY GELWKS REHPHYS+IIASR KHDDVQHLAKALS
Sbjct: 1561 RRVKDDSRARILKAYGLESFLEAWYSGELWKSFREHPHYSQIIASRSKHDDVQHLAKALS 1620

Query: 1621 DLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELHEI 1680
            DLSIGRQPQLWDELK CKTPLSIIVGEKDTKFKTIAQ+ILSQIDMGRKIKDEPVV+LHEI
Sbjct: 1621 DLSIGRQPQLWDELKCCKTPLSIIVGEKDTKFKTIAQQILSQIDMGRKIKDEPVVDLHEI 1680

Query: 1681 VEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            VEIPDSGHAAHLENPLAVINALS+FLIRSR QFSYNSDFSG
Sbjct: 1681 VEIPDSGHAAHLENPLAVINALSRFLIRSRKQFSYNSDFSG 1720

BLAST of Lag0040217.1 vs. ExPASy TrEMBL
Match: A0A1S3BFV3 (protein PHYLLO, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489211 PE=3 SV=1)

HSP 1 Score: 3073.9 bits (7968), Expect = 0.0e+00
Identity = 1526/1722 (88.62%), Postives = 1620/1722 (94.08%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+L+I KLA+ HPVRPFIR SFPRSKLFSTFAF PK + QPGSFRL QNS FKGVR+
Sbjct: 1    MQADTLTIHKLAVLHPVRPFIRSSFPRSKLFSTFAFIPKASRQPGSFRLSQNSSFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GA ELS+LEDVDL+VEMCIT T+PPALTLKQGLESI+EAIEK KLE PRSS GVF
Sbjct: 61   DSPVMGAMELSNLEDVDLLVEMCITHTLPPALTLKQGLESITEAIEKFKLESPRSSQGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS +AL+WFCCQPESSEV+PIFFLS EKD TIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSAKALLWFCCQPESSEVYPIFFLSNEKDPTIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQST KRYL+NDSAPIMAYGFVNANTGETSS+KN+A HSYFC+PQIEL EYEG
Sbjct: 181  SLSSTSSKQSTSKRYLMNDSAPIMAYGFVNANTGETSSLKNEADHSYFCIPQIELSEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            ISVLSATLAWSESFPCTFEE LHSLG SI+QIS NFPS E CQYKY+RSSLT+ KL+DGT
Sbjct: 241  ISVLSATLAWSESFPCTFEEALHSLGSSIYQISNNFPSSENCQYKYMRSSLTASKLVDGT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEMESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEHT 360
            +QMAYMKVLSVAGES RTGIMEMES+  HQFCIR+SPSVAVACNMLDHINET HS Q+H 
Sbjct: 301  NQMAYMKVLSVAGESVRTGIMEMESSLFHQFCIRVSPSVAVACNMLDHINETGHSEQQHA 360

Query: 361  NISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG 420
            NI+ALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG
Sbjct: 361  NINALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG 420

Query: 421  YAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQVN 480
            YA+GSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQDAGANQAINQVN
Sbjct: 421  YARGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQDAGANQAINQVN 480

Query: 481  HFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSPW 540
            HFGSFVRFFF+LP PTDQIPARMVLTTLDSAVHWATS+P GPVHINCPFREPLENSPSPW
Sbjct: 481  HFGSFVRFFFSLPAPTDQIPARMVLTTLDSAVHWATSSPYGPVHINCPFREPLENSPSPW 540

Query: 541  NLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHSE 600
            NL+CLNGLHIWLSSTE FTKYI+LEASPTSIDTFG MAEVLK+IHGARNG+LLLGSI SE
Sbjct: 541  NLSCLNGLHIWLSSTEVFTKYIRLEASPTSIDTFGHMAEVLKVIHGARNGVLLLGSIQSE 600

Query: 601  DEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKWL 660
            DEIWAA LLAKHISWPIVADVLSGLRLRKSLS FLE+E NFFF+DHLDHALLSDSVRKWL
Sbjct: 601  DEIWAAFLLAKHISWPIVADVLSGLRLRKSLSPFLEMENNFFFIDHLDHALLSDSVRKWL 660

Query: 661  KFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGCL 720
            KFDVIIQIGSRVTSKRVSK+LE+C+PCSYIMVD+HPGRHDPSHIVTHRIQSTVLEFVGC+
Sbjct: 661  KFDVIIQIGSRVTSKRVSKLLEECSPCSYIMVDKHPGRHDPSHIVTHRIQSTVLEFVGCI 720

Query: 721  LEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLGN 780
            L+  FP N+S  TATL+ALNMMVE EIQFQISANYSLSEP+VAQ+ISEAL  DSVLFLGN
Sbjct: 721  LKANFPLNKSKLTATLRALNMMVEWEIQFQISANYSLSEPEVAQVISEALSFDSVLFLGN 780

Query: 781  SMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVGC 840
            SMPIRDVDMYAYGW+KCNDSVAA PLNLQ+PF+W WTSGNRGASGIDGLLSTAVGFSVGC
Sbjct: 781  SMPIRDVDMYAYGWTKCNDSVAAIPLNLQMPFYWTWTSGNRGASGIDGLLSTAVGFSVGC 840

Query: 841  NKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAILD 900
            NKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVD AILD
Sbjct: 841  NKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDAAILD 900

Query: 901  QYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTFH 960
            Q+FHTSH++SLR+LC+AHGLKHLHVRT MELQDAL +S  EENDCIIEVESSIDANT FH
Sbjct: 901  QFFHTSHQVSLRNLCVAHGLKHLHVRTKMELQDALCLSHHEENDCIIEVESSIDANTAFH 960

Query: 961  SVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSID 1020
            SVLRKFTCQAVDHGLR+FSRLY EESVSPGLFLCKISRM+ TLFRIPLCAPPTT+SSSID
Sbjct: 961  SVLRKFTCQAVDHGLRIFSRLYSEESVSPGLFLCKISRMECTLFRIPLCAPPTTSSSSID 1020

Query: 1021 QVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSFI 1080
            QV R FFREGFILSLFLEDG+LGLGEVSPL+IHRENLLDVEEQLKCL+ ILKGAKISS I
Sbjct: 1021 QVRRGFFREGFILSLFLEDGNLGLGEVSPLDIHRENLLDVEEQLKCLIPILKGAKISSSI 1080

Query: 1081 PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENNL 1140
            PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVL+AIAGRK CSLL+VLQ+QLDEE NL
Sbjct: 1081 PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLHAIAGRKDCSLLDVLQHQLDEEKNL 1140

Query: 1141 TSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKKL 1200
            T+SSKVQICGLLDSGG+PS VA VAKTLVEEGF AIKLKVARQ NVMYDAAVVQEVRKKL
Sbjct: 1141 TTSSKVQICGLLDSGGTPSEVAYVAKTLVEEGFPAIKLKVARQRNVMYDAAVVQEVRKKL 1200

Query: 1201 GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDET 1260
            GNQIELRVDANRNWSYEEA+LFSSLVKDCGLQYIEEPVMDEDAI+KFCEESGLPVALDET
Sbjct: 1201 GNQIELRVDANRNWSYEEALLFSSLVKDCGLQYIEEPVMDEDAIIKFCEESGLPVALDET 1260

Query: 1261 VDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETGV 1320
            +D IQDNPVKELAKY HPGIVAIVIKPSV+GGFENAALVARWAQQHGKMAVVSAAFE+GV
Sbjct: 1261 IDRIQDNPVKELAKYAHPGIVAIVIKPSVVGGFENAALVARWAQQHGKMAVVSAAFESGV 1320

Query: 1321 GLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSGI 1380
            GLSGY+ LS YLE  NAE+RKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRI RDP SGI
Sbjct: 1321 GLSGYVHLSCYLEHQNAEVRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRIRRDPHSGI 1380

Query: 1381 IEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDNV 1440
            IEAS+AEAN+LLKNFQINQKFVCRK T+ QVR Y+L+VD KGFSY IKVLEVG RTNDNV
Sbjct: 1381 IEASIAEANQLLKNFQINQKFVCRKFTDGQVRSYRLSVDSKGFSYSIKVLEVGQRTNDNV 1440

Query: 1441 LVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEVV 1500
            L FLHGFLGTGEDW+TIMK VSGSARCISLDLPGHG STT+KNDCDVH V EPSFSMEVV
Sbjct: 1441 LFFLHGFLGTGEDWLTIMKGVSGSARCISLDLPGHGESTTEKNDCDVHGVVEPSFSMEVV 1500

Query: 1501 ANLLYKLIHHLAPGK--VNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR 1560
            A+L+YKLI HLAPGK  VN+VGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMAR+
Sbjct: 1501 ADLMYKLIQHLAPGKAIVNIVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARK 1560

Query: 1561 IRRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKAL 1620
            IRRVKDDSRARILK+YGLQSFLE WY GELWKSLREHPHYS+IIASRLKHDDVQHLAKAL
Sbjct: 1561 IRRVKDDSRARILKLYGLQSFLEAWYGGELWKSLREHPHYSKIIASRLKHDDVQHLAKAL 1620

Query: 1621 SDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELHE 1680
            S+LSIGRQPQLWDELK C+TPLSIIVGEKDTKFK IAQKILSQI+M +KIKDEPVV+LHE
Sbjct: 1621 SELSIGRQPQLWDELKCCETPLSIIVGEKDTKFKIIAQKILSQINMSKKIKDEPVVDLHE 1680

Query: 1681 IVEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            IVEIPDSGHAAHLENPLAV+NALS+FLIR RTQ S N D +G
Sbjct: 1681 IVEIPDSGHAAHLENPLAVVNALSRFLIRRRTQHSSNVDLAG 1722

BLAST of Lag0040217.1 vs. ExPASy TrEMBL
Match: A0A5A7SZ62 (Protein PHYLLO OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G005090 PE=3 SV=1)

HSP 1 Score: 3069.3 bits (7956), Expect = 0.0e+00
Identity = 1526/1723 (88.57%), Postives = 1620/1723 (94.02%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+L+I KLA+ HPVRPFIR SFPRSKLFSTFAF PK + QPGSFRL QNS FKGVR+
Sbjct: 1    MQADTLTIHKLAVLHPVRPFIRSSFPRSKLFSTFAFIPKASRQPGSFRLSQNSSFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GA ELS+LEDVDL+VEMCIT T+PPALTLKQGLESI+EAIEK KLE PRSS GVF
Sbjct: 61   DSPVMGAMELSNLEDVDLLVEMCITHTLPPALTLKQGLESITEAIEKFKLESPRSSQGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS +AL+WFCCQPESSEV+PIFFLS EKD TIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSAKALLWFCCQPESSEVYPIFFLSNEKDPTIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQST KRYL+NDSAPIMAYGFVNANTGETSS+KN+A HSYFC+PQIEL EYEG
Sbjct: 181  SLSSTSSKQSTSKRYLMNDSAPIMAYGFVNANTGETSSLKNEADHSYFCIPQIELSEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            ISVLSATLAWSESFPCTFEE LHSLG SI+QIS NFPS E CQYKY+RSSLT+ KL+DGT
Sbjct: 241  ISVLSATLAWSESFPCTFEEALHSLGSSIYQISNNFPSSENCQYKYMRSSLTASKLVDGT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEMESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEHT 360
            +QMAYMKVLSVAGES RTGIMEMES+  HQFCIR+SPSVAVACNMLDHINET HS Q+H 
Sbjct: 301  NQMAYMKVLSVAGESVRTGIMEMESSLFHQFCIRVSPSVAVACNMLDHINETGHSEQQHA 360

Query: 361  NISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG 420
            NI+ALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG
Sbjct: 361  NINALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG 420

Query: 421  YAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQ-V 480
            YA+GSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQDAGANQAINQ V
Sbjct: 421  YARGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPLELQDAGANQAINQVV 480

Query: 481  NHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSP 540
            NHFGSFVRFFF+LP PTDQIPARMVLTTLDSAVHWATS+P GPVHINCPFREPLENSPSP
Sbjct: 481  NHFGSFVRFFFSLPAPTDQIPARMVLTTLDSAVHWATSSPYGPVHINCPFREPLENSPSP 540

Query: 541  WNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHS 600
            WNL+CLNGLHIWLSSTE FTKYI+LEASPTSIDTFG MAEVLK+IHGARNG+LLLGSI S
Sbjct: 541  WNLSCLNGLHIWLSSTEVFTKYIRLEASPTSIDTFGHMAEVLKVIHGARNGVLLLGSIQS 600

Query: 601  EDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKW 660
            EDEIWAA LLAKHISWPIVADVLSGLRLRKSLS FLE+E NFFF+DHLDHALLSDSVRKW
Sbjct: 601  EDEIWAAFLLAKHISWPIVADVLSGLRLRKSLSPFLEMENNFFFIDHLDHALLSDSVRKW 660

Query: 661  LKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGC 720
            LKFDVIIQIGSRVTSKRVSK+LE+C+PCSYIMVD+HPGRHDPSHIVTHRIQSTVLEFVGC
Sbjct: 661  LKFDVIIQIGSRVTSKRVSKLLEECSPCSYIMVDKHPGRHDPSHIVTHRIQSTVLEFVGC 720

Query: 721  LLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLG 780
            +L+  FP N+S  TATL+ALNMMVE EIQFQISANYSLSEP+VAQ+ISEAL  DSVLFLG
Sbjct: 721  ILKANFPLNKSKLTATLRALNMMVEWEIQFQISANYSLSEPEVAQVISEALSFDSVLFLG 780

Query: 781  NSMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVG 840
            NSMPIRDVDMYAYGW+KCNDSVAA PLNLQ+PF+W WTSGNRGASGIDGLLSTAVGFSVG
Sbjct: 781  NSMPIRDVDMYAYGWTKCNDSVAAIPLNLQMPFYWTWTSGNRGASGIDGLLSTAVGFSVG 840

Query: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAIL 900
            CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVD AIL
Sbjct: 841  CNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDAAIL 900

Query: 901  DQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTF 960
            DQ+FHTSH++SLR+LC+AHGLKHLHVRT MELQDAL +S  EENDCIIEVESSIDANT F
Sbjct: 901  DQFFHTSHQVSLRNLCVAHGLKHLHVRTKMELQDALCLSHHEENDCIIEVESSIDANTAF 960

Query: 961  HSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSI 1020
            HSVLRKFTCQAVDHGLR+FSRLY EESVSPGLFLCKISRM+ TLFRIPLCAPPTT+SSSI
Sbjct: 961  HSVLRKFTCQAVDHGLRIFSRLYSEESVSPGLFLCKISRMECTLFRIPLCAPPTTSSSSI 1020

Query: 1021 DQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSF 1080
            DQV R FFREGFILSLFLEDG+LGLGEVSPL+IHRENLLDVEEQLKCL+ ILKGAKISS 
Sbjct: 1021 DQVRRGFFREGFILSLFLEDGNLGLGEVSPLDIHRENLLDVEEQLKCLIPILKGAKISSS 1080

Query: 1081 IPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENN 1140
            IPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVL+AIAGRK CSLL+VLQ+QLDEE N
Sbjct: 1081 IPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLHAIAGRKDCSLLDVLQHQLDEEKN 1140

Query: 1141 LTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKK 1200
            LT+SSKVQICGLLDSGG+PS VA VAKTLVEEGF AIKLKVARQ NVMYDAAVVQEVRKK
Sbjct: 1141 LTTSSKVQICGLLDSGGTPSEVAYVAKTLVEEGFPAIKLKVARQRNVMYDAAVVQEVRKK 1200

Query: 1201 LGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDE 1260
            LGNQIELRVDANRNWSYEEA+LFSSLVKDCGLQYIEEPVMDEDAI+KFCEESGLPVALDE
Sbjct: 1201 LGNQIELRVDANRNWSYEEALLFSSLVKDCGLQYIEEPVMDEDAIIKFCEESGLPVALDE 1260

Query: 1261 TVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETG 1320
            T+D IQDNPVKELAKY HPGIVAIVIKPSV+GGFENAALVARWAQQHGKMAVVSAAFE+G
Sbjct: 1261 TIDRIQDNPVKELAKYAHPGIVAIVIKPSVVGGFENAALVARWAQQHGKMAVVSAAFESG 1320

Query: 1321 VGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSG 1380
            VGLSGY+ LS YLE  NAE+RKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRI RDP SG
Sbjct: 1321 VGLSGYVHLSCYLEHQNAEVRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRIRRDPHSG 1380

Query: 1381 IIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDN 1440
            IIEAS+AEAN+LLKNFQINQKFVCRK T+ QVR Y+L+VD KGFSY IKVLEVG RTNDN
Sbjct: 1381 IIEASIAEANQLLKNFQINQKFVCRKFTDGQVRSYRLSVDSKGFSYSIKVLEVGQRTNDN 1440

Query: 1441 VLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEV 1500
            VL FLHGFLGTGEDW+TIMK VSGSARCISLDLPGHG STT+KNDCDVH V EPSFSMEV
Sbjct: 1441 VLFFLHGFLGTGEDWLTIMKGVSGSARCISLDLPGHGESTTEKNDCDVHGVVEPSFSMEV 1500

Query: 1501 VANLLYKLIHHLAPGK--VNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMAR 1560
            VA+L+YKLI HLAPGK  VN+VGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMAR
Sbjct: 1501 VADLMYKLIQHLAPGKAIVNIVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMAR 1560

Query: 1561 RIRRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKA 1620
            +IRRVKDDSRARILK+YGLQSFLE WY GELWKSLREHPHYS+IIASRLKHDDVQHLAKA
Sbjct: 1561 KIRRVKDDSRARILKLYGLQSFLEAWYGGELWKSLREHPHYSKIIASRLKHDDVQHLAKA 1620

Query: 1621 LSDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELH 1680
            LS+LSIGRQPQLWDELK C+TPLSIIVGEKDTKFK IAQKILSQI+M +KIKDEPVV+LH
Sbjct: 1621 LSELSIGRQPQLWDELKCCETPLSIIVGEKDTKFKIIAQKILSQINMSKKIKDEPVVDLH 1680

Query: 1681 EIVEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            EIVEIPDSGHAAHLENPLAV+NALS+FLIR RTQ S N D +G
Sbjct: 1681 EIVEIPDSGHAAHLENPLAVVNALSRFLIRRRTQHSSNVDLAG 1723

BLAST of Lag0040217.1 vs. ExPASy TrEMBL
Match: A0A0A0KQZ1 (MR_MLE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G607980 PE=3 SV=1)

HSP 1 Score: 3037.3 bits (7873), Expect = 0.0e+00
Identity = 1504/1722 (87.34%), Postives = 1604/1722 (93.15%), Query Frame = 0

Query: 1    MAADSLSIGKLALPHPVRPFIRGSFPRSKLFSTFAFFPKGTHQPGSFRLPQNSCFKGVRF 60
            M AD+L+  KLA+ HPVRPFIR SFPRSKL STFAF PK + QPGSF LPQNS FKGVR+
Sbjct: 1    MQADTLTAHKLAVLHPVRPFIRSSFPRSKLISTFAFIPKASRQPGSFHLPQNSSFKGVRY 60

Query: 61   DSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVF 120
            DSPV+GA ELSDLEDVDL+VEMCIT T+PPALTLKQGLESI+EAIEK KLE PRSS GVF
Sbjct: 61   DSPVMGAMELSDLEDVDLLVEMCITHTLPPALTLKQGLESITEAIEKFKLESPRSSQGVF 120

Query: 121  RFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEKDATIKSLYLNDTRGVFGIGTAIYFA 180
            RFQVAVPPS +AL+WFCCQPESSEV+PIFF+S EKD TIKSLYLNDTRGVFGIGTAIYFA
Sbjct: 121  RFQVAVPPSAKALLWFCCQPESSEVYPIFFVSNEKDPTIKSLYLNDTRGVFGIGTAIYFA 180

Query: 181  SLSSTSSKQSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQIELCEYEG 240
            SLSSTSSKQSTLKRY++NDSAPIMAYGFVNAN GETSS+KN+A HSY+CVPQIEL EYEG
Sbjct: 181  SLSSTSSKQSTLKRYVMNDSAPIMAYGFVNANNGETSSLKNEAGHSYYCVPQIELSEYEG 240

Query: 241  ISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNFPSREKCQYKYIRSSLTSLKLMDGT 300
            ISVLSATLAWSESFPCTFEE LHSLG SI+QISTNFPS E CQYKY+RS+LT+ KL+D T
Sbjct: 241  ISVLSATLAWSESFPCTFEEALHSLGSSIYQISTNFPSSENCQYKYLRSALTASKLVDRT 300

Query: 301  HQMAYMKVLSVAGESFRTGIMEMESAFSHQFCIRLSPSVAVACNMLDHINETRHSMQEHT 360
            +QMAYMKVLSVAGE  RTGIMEMES+  HQFCIRLSPSVAVACNMLDHINET HS QEH 
Sbjct: 301  NQMAYMKVLSVAGEGVRTGIMEMESSLFHQFCIRLSPSVAVACNMLDHINETGHSEQEHA 360

Query: 361  NISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFDERSLAFHAIG 420
            NI+ALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITC ACFDERSLAFHAIG
Sbjct: 361  NINALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCTACFDERSLAFHAIG 420

Query: 421  YAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDAGANQAINQVN 480
            YAKGSHSPAV+ITSSGTAVSNLLPAVVEASQDF PLLLLTADRP ELQ+AGANQAINQVN
Sbjct: 421  YAKGSHSPAVVITSSGTAVSNLLPAVVEASQDFLPLLLLTADRPSELQNAGANQAINQVN 480

Query: 481  HFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFREPLENSPSPW 540
            HFGSFVRFFF+LP PTDQ+PARMVLTTLDSAVHWATS+PCGPVHINCPFREPLENSPSPW
Sbjct: 481  HFGSFVRFFFSLPAPTDQLPARMVLTTLDSAVHWATSSPCGPVHINCPFREPLENSPSPW 540

Query: 541  NLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNGILLLGSIHSE 600
            NL+CLNGLHIW SSTE FTKYI+LEASPTS DTFG MAEVLK+IHGARNG+LLLGSI SE
Sbjct: 541  NLSCLNGLHIWSSSTEVFTKYIRLEASPTSTDTFGHMAEVLKVIHGARNGVLLLGSIQSE 600

Query: 601  DEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHALLSDSVRKWL 660
            DEIWAA LLAKHISWPIVADVLSGLRLRKSLS FLE++ NFFF+DHLDHALLSDSVRKWL
Sbjct: 601  DEIWAAFLLAKHISWPIVADVLSGLRLRKSLSPFLEMKNNFFFIDHLDHALLSDSVRKWL 660

Query: 661  KFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQSTVLEFVGCL 720
            KFDVIIQIGSRVTSKRVSK+LEDC+PCSYIMVD+HP RHDPSHIVTHRIQSTVLEFVGCL
Sbjct: 661  KFDVIIQIGSRVTSKRVSKLLEDCSPCSYIMVDKHPDRHDPSHIVTHRIQSTVLEFVGCL 720

Query: 721  LEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEALPSDSVLFLGN 780
            L+  FP N+S  TATL+ALNMMVE EIQFQISA+YSLSEP+VAQ+ISEAL  DSVLFLGN
Sbjct: 721  LKASFPLNKSKLTATLRALNMMVEWEIQFQISAHYSLSEPEVAQVISEALSFDSVLFLGN 780

Query: 781  SMPIRDVDMYAYGWSKCNDSVAARPLNLQVPFFWKWTSGNRGASGIDGLLSTAVGFSVGC 840
            SMPIRDVDMYAYGWSKCNDS AA PLNLQ+PF+W WTSGNRGASGIDGLLS+AVGFSVGC
Sbjct: 781  SMPIRDVDMYAYGWSKCNDSGAAIPLNLQMPFYWTWTSGNRGASGIDGLLSSAVGFSVGC 840

Query: 841  NKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLPIKDKVDPAILD 900
            NKRVLCV+GDVSFLHDTNGLAILN+RM RKPVTVVVINNNGGAIFSLLPIKDKVD AILD
Sbjct: 841  NKRVLCVLGDVSFLHDTNGLAILNKRMKRKPVTVVVINNNGGAIFSLLPIKDKVDAAILD 900

Query: 901  QYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEVESSIDANTTFH 960
            Q+FHTSH++SLR+LC+AHGLKHLHVRT  ELQDALFMS  EENDCIIEVESSIDANTTFH
Sbjct: 901  QFFHTSHQVSLRNLCVAHGLKHLHVRTKKELQDALFMSHHEENDCIIEVESSIDANTTFH 960

Query: 961  SVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLCAPPTTTSSSID 1020
            SVLRKFTCQAVDHGLR+FSRLY EESVSPGLFLCKISRM+ TLFRIPLCAPPTT+SSS D
Sbjct: 961  SVLRKFTCQAVDHGLRIFSRLYSEESVSPGLFLCKISRMECTLFRIPLCAPPTTSSSSFD 1020

Query: 1021 QVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLHILKGAKISSFI 1080
            QV R+FFREGFILSLFLEDGSLGLGEVSPL+IHRENLLDVEEQL CL+ ILKGAKISS I
Sbjct: 1021 QVRREFFREGFILSLFLEDGSLGLGEVSPLDIHRENLLDVEEQLNCLIPILKGAKISSSI 1080

Query: 1081 PLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNVLQYQLDEENNL 1140
            PLLRGSFSSW+FHELGIPPSSIYPSVRCGLEMAVL+AIAGRKGC LL+VLQ+QLDEE NL
Sbjct: 1081 PLLRGSFSSWVFHELGIPPSSIYPSVRCGLEMAVLHAIAGRKGCGLLDVLQHQLDEEKNL 1140

Query: 1141 TSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYDAAVVQEVRKKL 1200
             + SKVQICGLLDSGG+PS VA VAKTLVEEGF AIKLK  RQ NVMYDAAVVQEVRKKL
Sbjct: 1141 KTLSKVQICGLLDSGGTPSEVALVAKTLVEEGFPAIKLKETRQRNVMYDAAVVQEVRKKL 1200

Query: 1201 GNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCEESGLPVALDET 1260
            GNQIELRVDANRNWSYEEA+LFSSLVKDCGLQYIEEPVMDEDAI+KFCEESGLPVALDET
Sbjct: 1201 GNQIELRVDANRNWSYEEALLFSSLVKDCGLQYIEEPVMDEDAIIKFCEESGLPVALDET 1260

Query: 1261 VDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKMAVVSAAFETGV 1320
            +D IQDNPVKELAKY HPGIVAIVIKPSV+GGFENAAL+ARWAQQHGKMAVVSAAFE+GV
Sbjct: 1261 IDRIQDNPVKELAKYAHPGIVAIVIKPSVVGGFENAALIARWAQQHGKMAVVSAAFESGV 1320

Query: 1321 GLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRICRDPCSGI 1380
            GLSGY+ LS YLEL NAE+RKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLR  RDP SGI
Sbjct: 1321 GLSGYVHLSCYLELQNAEVRKLMNIQPAPSIAHGLGTYRWLEEDVTVNPLRFRRDPHSGI 1380

Query: 1381 IEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKVLEVGPRTNDNV 1440
            IEASVAEAN+LL+NFQINQK VCRK T+ QVR Y+L+VD KGFSY IKVLEVG RTNDNV
Sbjct: 1381 IEASVAEANQLLENFQINQKIVCRKFTDRQVRSYRLSVDSKGFSYSIKVLEVGQRTNDNV 1440

Query: 1441 LVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHDVEEPSFSMEVV 1500
            L FLHG LGTGEDW+TIMK VSGSARCISLDLPGHG STT+KNDCDVH VEEPSFSMEVV
Sbjct: 1441 LFFLHGCLGTGEDWLTIMKGVSGSARCISLDLPGHGESTTEKNDCDVHGVEEPSFSMEVV 1500

Query: 1501 ANLLYKLIHHLAPGK--VNLVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKMARR 1560
            A+LLYKLI HLAPGK  VN+VGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDK+AR+
Sbjct: 1501 ADLLYKLIQHLAPGKAIVNVVGYSMGARIAMYMALRFGDKIGRAVIISGSPGLKDKVARK 1560

Query: 1561 IRRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHDDVQHLAKAL 1620
            IRRVKDDSRAR+LK+YGLQSFLE WY GELWKSLREHPHYS+IIA RLKHDDVQ LAKAL
Sbjct: 1561 IRRVKDDSRARVLKLYGLQSFLEAWYGGELWKSLREHPHYSQIIARRLKHDDVQPLAKAL 1620

Query: 1621 SDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIKDEPVVELHE 1680
            S+LSIGRQPQLWDELK CKTPLSIIVGEKDTKFKTIAQ+ILSQI+  ++IKDEP V+LHE
Sbjct: 1621 SELSIGRQPQLWDELKCCKTPLSIIVGEKDTKFKTIAQQILSQINTSKRIKDEPAVDLHE 1680

Query: 1681 IVEIPDSGHAAHLENPLAVINALSKFLIRSRTQFSYNSDFSG 1721
            IVEIPDSGHAAHLENPLAV+NALS+FLIR RTQ S N D +G
Sbjct: 1681 IVEIPDSGHAAHLENPLAVVNALSRFLIRRRTQHSSNVDLAG 1722

BLAST of Lag0040217.1 vs. TAIR 10
Match: AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )

HSP 1 Score: 1866.3 bits (4833), Expect = 0.0e+00
Identity = 949/1658 (57.24%), Postives = 1224/1658 (73.82%), Query Frame = 0

Query: 56   KGVRFDSPVLGATELSDLEDVDLVVEMCITRTMPPALTLKQGLESISEAIEKLKLEPPRS 115
            +GV+FD P++      +L+D DLVV++C+TRT+PPALTL+ GLES+ EAI++LK  PP+S
Sbjct: 55   QGVQFDGPIMDRD--VNLDD-DLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKS 114

Query: 116  SSGVFRFQVAVPPSTRALMWFCCQPESSEVFPIFFLSKEK-DATIKSLYLNDTRGVFGIG 175
            SSGV RFQVAVPP  +AL WFC QP +S+VFP+FFLSK+  + + KSLY+ +  GVFGIG
Sbjct: 115  SSGVLRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLSKDTVEPSYKSLYVKEPHGVFGIG 174

Query: 176  TAIYFASLSSTSSK-QSTLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHSYFCVPQI 235
             A  F   SS  S   S +K +L ++SA + AYGF +    + S++ +    SYF VPQI
Sbjct: 175  NAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSKDGSSYFFVPQI 234

Query: 236  ELCEYEGISVLSATLAWSESFPCTFEEGLHSLGLSIHQISTNF-PSREKCQYKYIRSSLT 295
            EL E+E +S+L+ TLAW+ES   T E+ + S   SI Q+S++F P+ E   +K+++SSL 
Sbjct: 235  ELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSHFCPNVEDHWFKHLKSSLA 294

Query: 296  SLKLMD-GTHQMAYMKVLSVAGESFRTGIMEMESAFSH-QFCIRLSPSVAVACNMLDHIN 355
             L + +    +M +M   + +G   +  + E++S  S  QF  +LSP V  + NML+   
Sbjct: 295  KLSVEEIHPLEMEHMGFFTFSGRD-QADVKELKSIQSSCQFHCKLSPDVVFSNNMLNRET 354

Query: 356  ETRHSMQEHTNISALWASLIVEECSRLGLTYFCIAPGSRSSPLAIAAASHPLITCIACFD 415
            E  + +++  NI+A+WAS I+EEC+RLGLTYFC+APGSRSS LAIAAA+HPL TC+ACFD
Sbjct: 355  EVSNFLRDEANINAVWASAIIEECTRLGLTYFCVAPGSRSSHLAIAAANHPLTTCLACFD 414

Query: 416  ERSLAFHAIGYAKGSHSPAVIITSSGTAVSNLLPAVVEASQDFFPLLLLTADRPPELQDA 475
            ERSLAFHAIGYAKGS  PAVIITSSGTAVSNLLPAVVEAS+DF PLLLLTADRPPELQ  
Sbjct: 415  ERSLAFHAIGYAKGSLKPAVIITSSGTAVSNLLPAVVEASEDFLPLLLLTADRPPELQGV 474

Query: 476  GANQAINQVNHFGSFVRFFFNLPVPTDQIPARMVLTTLDSAVHWATSAPCGPVHINCPFR 535
            GANQAINQ+NHFGSFVRFFFNLP PTD IP RMVLTT+DSA+HWAT + CGPVH+NCPFR
Sbjct: 475  GANQAINQINHFGSFVRFFFNLPPPTDLIPVRMVLTTVDSALHWATGSACGPVHLNCPFR 534

Query: 536  EPLENSPSPWNLNCLNGLHIWLSSTEAFTKYIQLEASPTSIDTFGRMAEVLKIIHGARNG 595
            +PL+ SP+ W+ NCLNGL +W+S+ E FTKY Q+++  +   T G++ E+L++I  A+ G
Sbjct: 535  DPLDGSPTNWSSNCLNGLDMWMSNAEPFTKYFQVQSHKSDGVTTGQITEILQVIKEAKKG 594

Query: 596  ILLLGSIHSEDEIWAAVLLAKHISWPIVADVLSGLRLRKSLSSFLEIEKNFFFVDHLDHA 655
            +LL+G+IH+EDEIWA++LLAK + WP+VADVLSG+RLRK    F+E +    FVDHLDHA
Sbjct: 595  LLLIGAIHTEDEIWASLLLAKELMWPVVADVLSGVRLRKLFKPFVE-KLTHVFVDHLDHA 654

Query: 656  LLSDSVRKWLKFDVIIQIGSRVTSKRVSKMLEDCTPCSYIMVDEHPGRHDPSHIVTHRIQ 715
            L SDSVR  ++FDV+IQ+GSR+TSKRVS+MLE C P +YI+VD+HP RHDPSH+VTHR+Q
Sbjct: 655  LFSDSVRNLIEFDVVIQVGSRITSKRVSQMLEKCFPFAYILVDKHPCRHDPSHLVTHRVQ 714

Query: 716  STVLEFVGCLLEVGFPRNRSTWTATLQALNMMVESEIQFQISANYSLSEPQVAQIISEAL 775
            S +++F  C+L+  FP  RS     LQAL+  +  E+ FQISA  SL+EP VA ++S+AL
Sbjct: 715  SNIVQFANCVLKSRFPWRRSKLHGHLQALDGAIAREMSFQISAESSLTEPYVAHMLSKAL 774

Query: 776  PSDSVLFLGNSMPIRDVDMYAYGWSKCNDS-VAARPLNLQVPFFWKWTSGNRGASGIDGL 835
             S S LF+GNSMPIRDVDM  YG S  N S V    L+ ++P  W   +GNRGASGIDGL
Sbjct: 775  TSKSALFIGNSMPIRDVDM--YGCSSENSSHVVDMMLSAELPCQWIQVTGNRGASGIDGL 834

Query: 836  LSTAVGFSVGCNKRVLCVVGDVSFLHDTNGLAILNQRMTRKPVTVVVINNNGGAIFSLLP 895
            LS+A GF+VGC KRV+CVVGD+SFLHDTNGLAIL QR+ RKP+T++VINN GG IF LLP
Sbjct: 835  LSSATGFAVGCKKRVVCVVGDISFLHDTNGLAILKQRIARKPMTILVINNRGGGIFRLLP 894

Query: 896  IKDKVDPAILDQYFHTSHRISLRSLCMAHGLKHLHVRTNMELQDALFMSQREENDCIIEV 955
            I  K +P++L+QYF+T+H IS+ +LC+AHG++++HV T  EL+DALF+   EE DCI+EV
Sbjct: 895  IAKKTEPSVLNQYFYTAHDISIENLCLAHGVRYVHVGTKSELEDALFVPSVEEMDCIVEV 954

Query: 956  ESSIDANTTFHSVLRKFTCQAVDHGLRVFSRLYFEESVSPGLFLCKISRMDYTLFRIPLC 1015
            ESSI+AN   HS L +F  QA ++ L + S   F   +   + LC++S + Y+ +R+ LC
Sbjct: 955  ESSINANAIVHSTLERFARQAAENSLGIVSASSFLHPMIKNVLLCQVSGIQYSQYRVKLC 1014

Query: 1016 APPTTTSSSIDQVSRKFFREGFILSLFLEDGSLGLGEVSPLEIHRENLLDVEEQLKCLLH 1075
              PT  S    Q    F REGFILSL LEDGS+G GEV+PL  + ENL+DVE QL+ +LH
Sbjct: 1015 DRPTICSDEFSQ----FHREGFILSLTLEDGSIGYGEVAPLNSNVENLMDVEGQLQLVLH 1074

Query: 1076 ILKGAKISSFIPLLRGSFSSWIFHELGIPPSSIYPSVRCGLEMAVLNAIAGRKGCSLLNV 1135
            ++  AK S  +PLL GS SSWI+ ELGI  SSI+PSVRCGLEMA+LNA+A R   SLL +
Sbjct: 1075 LMNEAKFSYMLPLLNGSISSWIWSELGITASSIFPSVRCGLEMALLNAMAVRHDSSLLGI 1134

Query: 1136 LQYQLDEENNLTSSSKVQICGLLDSGGSPSVVARVAKTLVEEGFTAIKLKVARQGNVMYD 1195
            L YQ  EEN       VQIC LLDS G+P  VA VA+ LV+EGF+AIKLKV R+ + + D
Sbjct: 1135 LHYQ-KEENGSAQPHSVQICALLDSEGTPLEVAYVARKLVQEGFSAIKLKVGRRVSSVQD 1194

Query: 1196 AAVVQEVRKKLGNQIELRVDANRNWSYEEAILFSSLVKDCGLQYIEEPVMDEDAIVKFCE 1255
            A V+QEVR+ +G QIELR DAN  W++EEA  F  LV  C L+YIEEPV ++D +++F E
Sbjct: 1195 ALVMQEVRRAVGVQIELRADANCRWTFEEAREFGLLVNSCNLKYIEEPVQNKDDLIRFHE 1254

Query: 1256 ESGLPVALDETVDHIQDNPVKELAKYVHPGIVAIVIKPSVIGGFENAALVARWAQQHGKM 1315
            E+GLPVALDET+D  ++ P++ L KY HPGIVA+VIKPSV+GGFENAAL+ARWAQQHGKM
Sbjct: 1255 ETGLPVALDETLDDFEECPLRMLTKYTHPGIVAVVIKPSVVGGFENAALIARWAQQHGKM 1314

Query: 1316 AVVSAAFETGVGLSGYIQLSSYLELHNAELRKLMNIQPAPSIAHGLGTYRWLEEDVTVNP 1375
            AV+SAA+E+G+GLS YI  +SYLE+ N +          PS+AHGLGTYRWL EDV +N 
Sbjct: 1315 AVISAAYESGLGLSAYILFASYLEMENVKASTEQKQGTPPSVAHGLGTYRWLSEDVMMNT 1374

Query: 1376 LRICRDPCSGIIEASVAEANRLLKNFQINQKFVCRKSTEEQVRRYQLTVDLKGFSYLIKV 1435
            L I R P SG +E  +A+A+R LK+ +IN   + R S    VRRY+L VD+ GFS+ I+V
Sbjct: 1375 LGIFRSPYSGFVEGFIADASRNLKDVKINNDVIVRTSKGIPVRRYELRVDVDGFSHFIRV 1434

Query: 1436 LEVGPRTNDNVLVFLHGFLGTGEDWITIMKAVSGSARCISLDLPGHGGSTTQKNDCDVHD 1495
             +VG     +V +FLHGFLGTGE+WI IM  +SGSARCIS+D+PGHG S  Q +  +   
Sbjct: 1435 HDVGENAEGSVALFLHGFLGTGEEWIPIMTGISGSARCISVDIPGHGRSRVQSHASETQ- 1494

Query: 1496 VEEPSFSMEVVANLLYKLIHHLAPGKVNLVGYSMGARIAMYMALRFGDKIGRAVIISGSP 1555
               P+FSME++A  LYKLI  + PGKV +VGYSMGARIA+YMALRF +KI  AV++SGSP
Sbjct: 1495 -TSPTFSMEMIAEALYKLIEQITPGKVTIVGYSMGARIALYMALRFSNKIEGAVVVSGSP 1554

Query: 1556 GLKDKMARRIRRVKDDSRARILKVYGLQSFLEMWYCGELWKSLREHPHYSEIIASRLKHD 1615
            GLKD +AR+IR   DDS+AR++   GL  F+E WY G LWKSLR HPH+S+I ASRL H 
Sbjct: 1555 GLKDPVARKIRSATDDSKARMMVDNGLYIFIENWYNGGLWKSLRNHPHFSKIAASRLLHG 1614

Query: 1616 DVQHLAKALSDLSIGRQPQLWDELKYCKTPLSIIVGEKDTKFKTIAQKILSQIDMGRKIK 1675
            DV  +AK LSDLS GRQP LW+EL+ C T +S++ GEKD K+K IA ++  ++   +K  
Sbjct: 1615 DVPSVAKLLSDLSSGRQPSLWEELEDCDTNISLVFGEKDVKYKQIATRMYREMSKSKK-- 1674

Query: 1676 DEPVVELHEIVEIPDSGHAAHLENPLAVINALSKFLIR 1708
               V  + EIVEIP++GHA HLE+PL VI AL KFL R
Sbjct: 1675 --SVNNIIEIVEIPEAGHAVHLESPLRVILALRKFLTR 1694

BLAST of Lag0040217.1 vs. TAIR 10
Match: AT1G74710.1 (ADC synthase superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 9.4e-17
Identity = 62/210 (29.52%), Postives = 101/210 (48.10%), Query Frame = 0

Query: 85  TRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVFRFQVAVPPSTRALMWFCCQPESSE 144
           TRTM   L+     E +  A+ +LK +PP  SSGV R QV +     A+ W   Q   +E
Sbjct: 64  TRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPIDQQIGAIDWLQAQ---NE 123

Query: 145 VFPIFFLSKEKDATIKSLYLN------------------DTRGVFGIGTAIYFASLSSTS 204
           + P  F S+  D     L L+                  +   V GIG+A++F  L   S
Sbjct: 124 IQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAGIGSAVFFRDLDPFS 183

Query: 205 SKQ-STLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHS-YFCVPQIELCEYEGISVL 264
                +++R+L + S  I AYG +  +     +++ +   + YF VPQ+E  E+ G S+L
Sbjct: 184 HDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSVPQVEFNEFGGSSML 243

Query: 265 SATLAWSESFPCTFEEGLHSLGLSIHQIST 275
           +AT+AW +    T E  + +L  ++ Q+S+
Sbjct: 244 AATIAWDDELSWTLENAIEALQETMLQVSS 270

BLAST of Lag0040217.1 vs. TAIR 10
Match: AT1G74710.2 (ADC synthase superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 9.4e-17
Identity = 62/210 (29.52%), Postives = 101/210 (48.10%), Query Frame = 0

Query: 85  TRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVFRFQVAVPPSTRALMWFCCQPESSE 144
           TRTM   L+     E +  A+ +LK +PP  SSGV R QV +     A+ W   Q   +E
Sbjct: 64  TRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPIDQQIGAIDWLQAQ---NE 123

Query: 145 VFPIFFLSKEKDATIKSLYLN------------------DTRGVFGIGTAIYFASLSSTS 204
           + P  F S+  D     L L+                  +   V GIG+A++F  L   S
Sbjct: 124 IQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAGIGSAVFFRDLDPFS 183

Query: 205 SKQ-STLKRYLVNDSAPIMAYGFVNANTGETSSMKNDAVHS-YFCVPQIELCEYEGISVL 264
                +++R+L + S  I AYG +  +     +++ +   + YF VPQ+E  E+ G S+L
Sbjct: 184 HDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSVPQVEFNEFGGSSML 243

Query: 265 SATLAWSESFPCTFEEGLHSLGLSIHQIST 275
           +AT+AW +    T E  + +L  ++ Q+S+
Sbjct: 244 AATIAWDDELSWTLENAIEALQETMLQVSS 270

BLAST of Lag0040217.1 vs. TAIR 10
Match: AT1G18870.1 (isochorismate synthase 2 )

HSP 1 Score: 84.7 bits (208), Expect = 7.9e-16
Identity = 60/202 (29.70%), Postives = 97/202 (48.02%), Query Frame = 0

Query: 85  TRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVFRFQVAVPPSTRALMWFCCQPESSE 144
           TRT+    +     E +  A+  LK +PP  SSG+ R QV +     A+ W   Q   +E
Sbjct: 67  TRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIEQKIGAIDWLHAQ---NE 126

Query: 145 VFPIFFLSKEKDATIKSLYL----------NDTRGVFGIGTAIYFASLSSTSSKQ-STLK 204
           + P  F S+  D+    L            ++   V GIG+A++F  L   S     +++
Sbjct: 127 ILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFRDLDPFSHDDWRSIR 186

Query: 205 RYLVNDSAPIMAYGFVNAN-TGETSSMKNDAVHSYFCVPQIELCEYEGISVLSATLAWSE 264
           R+L + S  I AYG +  + TG+ +         YF VPQ+E  E+ G S+L+AT+AW  
Sbjct: 187 RFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEFGGSSMLAATVAWDN 246

Query: 265 SFPCTFEEGLHSLGLSIHQIST 275
               T E  + +L  ++ Q+S+
Sbjct: 247 ELSWTLENAIEALQETMLQVSS 265

BLAST of Lag0040217.1 vs. TAIR 10
Match: AT1G18870.2 (isochorismate synthase 2 )

HSP 1 Score: 84.7 bits (208), Expect = 7.9e-16
Identity = 60/202 (29.70%), Postives = 97/202 (48.02%), Query Frame = 0

Query: 85  TRTMPPALTLKQGLESISEAIEKLKLEPPRSSSGVFRFQVAVPPSTRALMWFCCQPESSE 144
           TRT+    +     E +  A+  LK +PP  SSG+ R QV +     A+ W   Q   +E
Sbjct: 17  TRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIEQKIGAIDWLHAQ---NE 76

Query: 145 VFPIFFLSKEKDATIKSLYL----------NDTRGVFGIGTAIYFASLSSTSSKQ-STLK 204
           + P  F S+  D+    L            ++   V GIG+A++F  L   S     +++
Sbjct: 77  ILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFRDLDPFSHDDWRSIR 136

Query: 205 RYLVNDSAPIMAYGFVNAN-TGETSSMKNDAVHSYFCVPQIELCEYEGISVLSATLAWSE 264
           R+L + S  I AYG +  + TG+ +         YF VPQ+E  E+ G S+L+AT+AW  
Sbjct: 137 RFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEFGGSSMLAATVAWDN 196

Query: 265 SFPCTFEEGLHSLGLSIHQIST 275
               T E  + +L  ++ Q+S+
Sbjct: 197 ELSWTLENAIEALQETMLQVSS 215