Lag0037699.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0037699.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	60S ribosomal protein L27
Location	chr2: 8470527 .. 8470934 (+)
Sequence length	408
RNA-Seq Expression	Lag0037699.1
Synteny	Lag0037699.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAATTTATCAAGCCGAACAAGGCAGTTATTGTTCTGCAAGGCCGATTCGCCGGCCGAAAGGCTGTGATCGTCCGCAACTTCGATGACGGAACCCGCGATCGCCCCTACGGCCATTGTTTGGTTGCAGGGATCAAAAAGTACCCGAGCAAGGTCATTCGCAAGGATTCAACGAAGAAGACGGCGAAGAAATCGAGAGTGAAGGCCTTCATCAAACTTGTGAACTACCAGCACCTCATGCCGACAAGGTACACGCTCGACGTCGATCTCAAGGATGTGGTTAATGTGGATTGTTTGCAGTCGAAGGGATCAAAGGTCGCAGCCGCAAAGGAAATCAAGAAGAGGTTGGAGGACAGGTTTAAGACTGGGAAGAACAGGTGGTTTTTTACCAAGCTTAGGTTCTGA

mRNA sequence

ATGGTGAAATTTATCAAGCCGAACAAGGCAGTTATTGTTCTGCAAGGCCGATTCGCCGGCCGAAAGGCTGTGATCGTCCGCAACTTCGATGACGGAACCCGCGATCGCCCCTACGGCCATTGTTTGGTTGCAGGGATCAAAAAGTACCCGAGCAAGGTCATTCGCAAGGATTCAACGAAGAAGACGGCGAAGAAATCGAGAGTGAAGGCCTTCATCAAACTTGTGAACTACCAGCACCTCATGCCGACAAGGTACACGCTCGACGTCGATCTCAAGGATGTGGTTAATGTGGATTGTTTGCAGTCGAAGGGATCAAAGGTCGCAGCCGCAAAGGAAATCAAGAAGAGGTTGGAGGACAGGTTTAAGACTGGGAAGAACAGGTGGTTTTTTACCAAGCTTAGGTTCTGA

Coding sequence (CDS)

ATGGTGAAATTTATCAAGCCGAACAAGGCAGTTATTGTTCTGCAAGGCCGATTCGCCGGCCGAAAGGCTGTGATCGTCCGCAACTTCGATGACGGAACCCGCGATCGCCCCTACGGCCATTGTTTGGTTGCAGGGATCAAAAAGTACCCGAGCAAGGTCATTCGCAAGGATTCAACGAAGAAGACGGCGAAGAAATCGAGAGTGAAGGCCTTCATCAAACTTGTGAACTACCAGCACCTCATGCCGACAAGGTACACGCTCGACGTCGATCTCAAGGATGTGGTTAATGTGGATTGTTTGCAGTCGAAGGGATCAAAGGTCGCAGCCGCAAAGGAAATCAAGAAGAGGTTGGAGGACAGGTTTAAGACTGGGAAGAACAGGTGGTTTTTTACCAAGCTTAGGTTCTGA

Protein sequence

MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTKKTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDRFKTGKNRWFFTKLRF

Homology

[Close]

BLAST of Lag0037699.1 vs. NCBI nr
Match: XP_022981139.1 (60S ribosomal protein L27-like [Cucurbita maxima])

HSP 1 Score: 265.8 bits (678), Expect = 2.1e-67
Identity = 132/135 (97.78%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQH+MPTRYTLDVDLKDVVN DCLQSKGSKVAAAKEIKKRLEDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHIMPTRYTLDVDLKDVVNADCLQSKGSKVAAAKEIKKRLEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. NCBI nr
Match: XP_023523697.1 (60S ribosomal protein L27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264.2 bits (674), Expect = 6.0e-67
Identity = 131/135 (97.04%), Postives = 133/135 (98.52%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQH+MPTRYTLDVDLKDVVN DCLQSKGSKV AAKEIKKRLEDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHIMPTRYTLDVDLKDVVNADCLQSKGSKVVAAKEIKKRLEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. NCBI nr
Match: XP_022940098.1 (60S ribosomal protein L27-3-like [Cucurbita moschata])

HSP 1 Score: 262.7 bits (670), Expect = 1.8e-66
Identity = 131/135 (97.04%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQH+MPTRYTLDVDLKDVVNVDCLQSK SKVAA+KEIKKRLEDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHIMPTRYTLDVDLKDVVNVDCLQSKVSKVAASKEIKKRLEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. NCBI nr
Match: XP_022143316.1 (60S ribosomal protein L27-like [Momordica charantia])

HSP 1 Score: 262.3 bits (669), Expect = 2.3e-66
Identity = 129/135 (95.56%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAV+VLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGI KYPS+VIRKDSTK
Sbjct: 1   MVKFIKPNKAVVVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGISKYPSRVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKG+KVAAAKEIKKR+E+R
Sbjct: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGTKVAAAKEIKKRMEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. NCBI nr
Match: XP_038899503.1 (60S ribosomal protein L27-3-like [Benincasa hispida])

HSP 1 Score: 261.5 bits (667), Expect = 3.9e-66
Identity = 130/135 (96.30%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFD+GTRDRPYG+CLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDEGTRDRPYGYCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAA+KEIKKR EDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAASKEIKKRFEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. ExPASy Swiss-Prot
Match: P51419 (60S ribosomal protein L27-3 OS=Arabidopsis thaliana OX=3702 GN=RPL27C PE=2 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 2.8e-59
Identity = 111/135 (82.22%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKF+K NKAVI+LQGR+AG+KAVI+++FDDG RDRPYGHCLVAG+KKYPSKVIRKDS K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVK FIKLVNYQHLMPTRYTLDVDLK+V  +D LQSK  KVAA KE K +LE+R
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. ExPASy Swiss-Prot
Match: Q05462 (60S ribosomal protein L27 OS=Pisum sativum OX=3888 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 2.8e-59
Identity = 112/135 (82.96%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKF+KPNKAVI+LQGR+AG+KAVIV+ FDDGTR++PYGHCLVAGIKK+PSKVI+KDS K
Sbjct: 1   MVKFLKPNKAVILLQGRYAGKKAVIVKTFDDGTREKPYGHCLVAGIKKFPSKVIKKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAF+KLVNYQHLMPTRYTLDVDLKD V  D LQSK  KV A KE KK LE+R
Sbjct: 61  KTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVVPDVLQSKDKKVTALKETKKSLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. ExPASy Swiss-Prot
Match: Q8LCL3 (60S ribosomal protein L27-2 OS=Arabidopsis thaliana OX=3702 GN=RPL27B PE=2 SV=2)

HSP 1 Score: 222.2 bits (565), Expect = 3.4e-57
Identity = 108/135 (80.00%), Postives = 121/135 (89.63%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKF+K NKAVI+LQGR+AG+KAVI+++FDDGT DR YGHCLVAG+KKYPSKVIRKDS K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVK FIKLVNYQHLMPTRYTLDVDLK+V  +D L+SK  KV A KE K +LE+R
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. ExPASy Swiss-Prot
Match: Q9SKX8 (60S ribosomal protein L27-1 OS=Arabidopsis thaliana OX=3702 GN=RPL27A PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 5.1e-53
Identity = 99/135 (73.33%), Postives = 117/135 (86.67%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVK +KP KAVI+LQGR+ G+KAVIV++FDDGT ++ YGHCLVAG+KKYPSKVIRKDS K
Sbjct: 1   MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVK F K++NYQH+MPTRYTLD+DLK+VV+ D + SK  KV A KE K + E+R
Sbjct: 61  KTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of Lag0037699.1 vs. ExPASy Swiss-Prot
Match: P41101 (60S ribosomal protein L27 OS=Solanum tuberosum OX=4113 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.0e-52
Identity = 105/139 (75.54%), Postives = 116/139 (83.45%), Query Frame = 0


Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           M KF+KPNKAVI+LQG++AGRKAVIVR FD+GTRDRPYGHCLVAGI +YP KVIRKDS K
Sbjct: 1   MEKFLKPNKAVILLQGKYAGRKAVIVRAFDEGTRDRPYGHCLVAGISRYPKKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDL----KDVVNVDCLQSKGSKVAAAKEIKKR 120
           K  KKSRVKAFIKLVNY H+MPTRYTLD DL    KDVVN D LQ++  KV  A+  K R
Sbjct: 61  KAGKKSRVKAFIKLVNYNHIMPTRYTLDEDLKDVVKDVVNADVLQARDKKV-TARRTKAR 120

Query: 121 LEDRFKTGKNRWFFTKLRF 136
           L +RFKTGKNRWFFTKLRF
Sbjct: 121 LAERFKTGKNRWFFTKLRF 138

The following BLAST results are available for this feature:

BLAST of Lag0037699.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022981139.1	2.1e-67	97.78	60S ribosomal protein L27-like [Cucurbita maxima]	[more]
XP_023523697.1	6.0e-67	97.04	60S ribosomal protein L27-like [Cucurbita pepo subsp. pepo]	[more]
XP_022940098.1	1.8e-66	97.04	60S ribosomal protein L27-3-like [Cucurbita moschata]	[more]
XP_022143316.1	2.3e-66	95.56	60S ribosomal protein L27-like [Momordica charantia]	[more]
XP_038899503.1	3.9e-66	96.30	60S ribosomal protein L27-3-like [Benincasa hispida]	[more]

BLAST of Lag0037699.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51419	2.8e-59	82.22	60S ribosomal protein L27-3 OS=Arabidopsis thaliana OX=3702 GN=RPL27C PE=2 SV=2	[more]
Q05462	2.8e-59	82.96	60S ribosomal protein L27 OS=Pisum sativum OX=3888 GN=RPL27 PE=2 SV=1	[more]
Q8LCL3	3.4e-57	80.00	60S ribosomal protein L27-2 OS=Arabidopsis thaliana OX=3702 GN=RPL27B PE=2 SV=2	[more]
Q9SKX8	5.1e-53	73.33	60S ribosomal protein L27-1 OS=Arabidopsis thaliana OX=3702 GN=RPL27A PE=2 SV=1	[more]
P41101	2.0e-52	75.54	60S ribosomal protein L27 OS=Solanum tuberosum OX=4113 GN=RPL27 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005824	KOW	SMART	SM00739	kow_9	coord: 4..31 e-value: 0.0031 score: 26.8
IPR038655	Ribosomal protein L27e superfamily	GENE3D	2.30.30.770		coord: 1..135 e-value: 8.3E-61 score: 206.0
IPR001141	Ribosomal protein L27e	PFAM	PF01777	Ribosomal_L27e	coord: 52..135 e-value: 1.7E-31 score: 108.4
IPR001141	Ribosomal protein L27e	PANTHER	PTHR10497	60S RIBOSOMAL PROTEIN L27	coord: 1..135
None	No IPR available	PANTHER	PTHR10497:SF16	60S RIBOSOMAL PROTEIN L27	coord: 1..135
IPR018262	Ribosomal protein L27e, conserved site	PROSITE	PS01107	RIBOSOMAL_L27E	coord: 124..135
IPR041991	Eukaryotic Ribosomal Protein L27, KOW domain	CDD	cd06090	KOW_RPL27	coord: 6..88 e-value: 7.31423E-43 score: 134.233
IPR008991	Translation protein SH3-like domain superfamily	SUPERFAMILY	50104	Translation proteins SH3-like domain	coord: 2..135

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0037699	Lag0037699	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0037699.1.exon1	Lag0037699.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0037699.1	cds.Lag0037699.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0037699.1	Lag0037699.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
cellular_component	GO:0022625	cytosolic large ribosomal subunit
molecular_function	GO:0003735	structural constituent of ribosome