Lag0031509.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0031509.1 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 490.0 bits (1260), Expect = 3.3e-134
Identity = 242/575 (42.09%), Postives = 336/575 (58.43%), Query Frame = 0

Query: 115  GWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIH 174
            GWK K FS+ GKEVL+K++ QAIPCYTM+CFRLP+ LI+E H   ARFWW  S+E+++IH
Sbjct: 571  GWKAKLFSIGGKEVLIKAVAQAIPCYTMSCFRLPKRLIREFHHITARFWWGSSKEDKKIH 630

Query: 175  WLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLE 234
            W++W+SL LPKC GG+GFR++ELFN+ALLAKQCWR+L  P+S+L  VLKG YF    F+E
Sbjct: 631  WVAWNSLYLPKCEGGMGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFME 690

Query: 235  ADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPVLSPA 294
            A I   PS++W S+LWGR+LL +G RWRIGNG +  IYG NW+PN+ +L+I S+P L   
Sbjct: 691  AKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLV 750

Query: 295  STVSELFT-ASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSVKSG 354
            S VS L     GGW   ++R  F   + + IL IP+  G+ EDRLIW++EK G +SV+SG
Sbjct: 751  SRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSG 810

Query: 355  YRLA-HTLATQDRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLPTKVNLLKRG 414
            Y++A         P SS+SE VR WW+G W++++PNK + FLWRLC DRLPT  NL KRG
Sbjct: 811  YKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRG 870

Query: 415  LSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEIIGAMKDNLT 474
            + ++  C  C  + ED +HLFW C   +++W+ SKF     PF  LR         ++L+
Sbjct: 871  VEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKL-SPFLILR------ESHESLS 930

Query: 475  GLDFELVVIFWWSVWNLRNNLSWGGQPD-----GRDLWAYSSDYLSAFHE---------- 534
              DFE + +  W +WN RN  ++          G +L  +++ Y   F E          
Sbjct: 931  KADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRV 990

Query: 535  ---------------LKLNTDASVRPVQGEAGGGCVLRGADGEVFMTACLSLQRCWSVDL 594
                            K+NTDAS       AG G ++    G+V   A   L+   SVD+
Sbjct: 991  TNTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDM 1050

Query: 595  AEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDDIRRILSPWDN 654
            AE  A   G+QLA ++                 +H  L D+SE G ++   +   +   +
Sbjct: 1051 AEAIAAVEGLQLASEIG----------------MHPALEDLSETGEIVLKAKNFWTQSLH 1110

Query: 655  GKVLFTPRQGNKVAHALARLAFSYAD-RVWLEEWP 657
                F  R+GNK AH LAR A    +  +W+E+WP
Sbjct: 1111 ASFNFVKREGNKAAHMLARRALLLHEFSIWMEDWP 1122

BLAST of Lag0031509.1 vs. NCBI nr
Match: ONI01138.1 (hypothetical protein PRUPE_6G123900 [Prunus persica])

HSP 1 Score: 448.0 bits (1151), Expect = 1.4e-121
Identity = 255/698 (36.53%), Postives = 367/698 (52.58%), Query Frame = 0

Query: 24   LLCGAERRSQITGFRVDALAHRF-RTYFWDDNLLFFRAIGSEALVIRELLVRYERALGQT 83
            LL GAERR  + G +V   A       F DD++LF +A   + + +  L   YE   GQ 
Sbjct: 320  LLRGAERRGDLVGVQVARGAPSVTHLLFADDSILFMKATNKDCMALETLFQTYEEVTGQQ 379

Query: 84   INYEKSVIALA------------------------------RIRGKRLNNISAKFFPCPA 143
            INY KS ++L+                               I GK    +         
Sbjct: 380  INYSKSALSLSPNATRADFDMIEGVLNVPVVRCHENYLGLPTIAGKGRKQLFQHLKDKLW 439

Query: 144  AH--GWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEE 203
             H  GWK K  S AGKE+L+K+++QAIP Y+M+CFR+P+GL KE++  MARFWW  ++++
Sbjct: 440  KHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFRIPKGLCKELNGIMARFWWAKAKDK 499

Query: 204  RRIHWLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQS 263
            R IHW+ W+ LC  K  GGLGFR++E FNQALLAKQCWR+L+ P SL+  + +  Y P  
Sbjct: 500  RGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRTPESLVARIFRARYHPSV 559

Query: 264  GFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPV 323
             FLEA++G+ PSF+W SL WG+ELL +G RWR+G+G +  +Y   WLP     +I S P 
Sbjct: 560  PFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGSGVSIQVYTDKWLPAPSCFKIMSPPQ 619

Query: 324  LSPASTVSELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSV 383
            L  ++ V +LFT+SG WN  LL+ IF   + +AIL+IPL   +G D LIWH+E++G +SV
Sbjct: 620  LPLSTRVCDLFTSSGQWNVPLLKDIFWDQEVDAILQIPLASLAGHDCLIWHYERNGMYSV 679

Query: 384  KSGYRLAHTLATQDRPGSSNSERVRM---WWSGLWRLNVPNKHRFFLWRLCHDRLPTKVN 443
            KSGYRLA     +D+     S RV +   +W  +W L +PNK +FFLWR   D LP    
Sbjct: 680  KSGYRLAG--LEKDKMSGEPSARVDLNSKFWKKIWALKIPNKIKFFLWRCAWDFLPCGQI 739

Query: 444  LLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEIIGAM 503
            L  R ++ +P+C  C   AE  LH  W C   K +W  S +    + +    F E+  A+
Sbjct: 740  LFNRKIAPTPICPKCHRKAESVLHAVWLCEAAKEVWRNSAWGNVCEVWRVNSFRELWHAL 799

Query: 504  KDNLTGLDFELVVIFWWSVWNLRNNLSWGGQPDG--------RDLWAYSSDYLSAFHELK 563
            + + +G +  L     W +WN RN+  + G+ +           L    SD  +  H + 
Sbjct: 800  QLSSSGEEQGLFAYLCWGLWNRRNSFIFEGKSETAIQLLSRMTKLAQEFSDANNILHTIH 859

Query: 564  LNTDASVRPVQG------------EAGGGCVLRGADGEVFMTACL-SLQRCWSVDLAEGW 623
                +   P+QG              G G V+R A+GE FM AC+  +   +     E  
Sbjct: 860  GRQSSPQAPLQGWRPPPAVKSGDSVRGVGVVVRNANGE-FMAACVRRIHASYGARQTELM 919

Query: 624  AVYRGIQLARQLAFIDFVVETDSLR-LVKILHGELHD------VSEVGLLMDDIRRILSP 657
            A   G++ A  + F D ++E D+   L  I   E ++      + EV  L+++ R ++  
Sbjct: 920  ATIEGLRFAIDMGFTDAILEMDAQDCLNSIFSTEEYNGIDGPLLEEVNYLLNNFRAVVCH 979

BLAST of Lag0031509.1 vs. NCBI nr
Match: VVA32947.1 (PREDICTED: retrotransposon [Prunus dulcis])

HSP 1 Score: 446.4 bits (1147), Expect = 4.2e-121
Identity = 254/706 (35.98%), Postives = 366/706 (51.84%), Query Frame = 0

Query: 24   LLCGAERRSQITGFRV----DALAHRFRTYFWDDNLLFFRAIGSEALVIRELLVRYERAL 83
            LL GAERR  + G +V     ++ H     F DD++LF +A       +  L   YE   
Sbjct: 586  LLRGAERRGDLVGVQVARGGPSVTH---LLFADDSILFMKATNEACRALETLFQTYEEVS 645

Query: 84   GQTINYEKSVIALA---------RIRGKRLN----NISAKFFPCPA-------------- 143
            GQ INY KS  +L+          I+G  LN        K+   P               
Sbjct: 646  GQQINYSKSAFSLSPNATRADFDMIKGV-LNVPVVQCHEKYLGLPTIAGKGRKQLFQHLK 705

Query: 144  ------AHGWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNG 203
                    GWK K  S AGKE+L+K+++QAIP Y+M+CFR+P+GL KE++  MARFWW  
Sbjct: 706  DKLWKHISGWKEKLLSRAGKEILMKAVLQAIPTYSMSCFRIPKGLCKELNGIMARFWWAK 765

Query: 204  SEEERRIHWLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCY 263
            ++++R IHW+ W+ LC  K  GGLGFR++E FNQALLAKQCWR+L+ P SL+  + +  Y
Sbjct: 766  AKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRTPESLVARIFRARY 825

Query: 264  FPQSGFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQ 323
             P   FLEA++G+ PSF+W SL WG+ELL +G RWR+GNG +  +Y   WLP     +I 
Sbjct: 826  HPSVPFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGNGVSIQVYTDKWLPAPSFFKIM 885

Query: 324  SAPVLSPASTVSELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHG 383
            S P L  ++ V +LFT+SG WN  LL+ IF   + +A L+IPL   +G D LIWH+E++G
Sbjct: 886  SPPQLPLSTLVCDLFTSSGQWNVPLLKDIFWDQEVDAKLQIPLASLAGHDCLIWHYERNG 945

Query: 384  NFSVKSGYRLAHTLATQDRPGSSNSERVRM---WWSGLWRLNVPNKHRFFLWRLCHDRLP 443
             +SVKSGYRLA     +D+     S RV +   +W  +W L +PNK +FFLWR   D LP
Sbjct: 946  MYSVKSGYRLA--CLEKDKMSGEPSVRVDLNSKFWKKIWALKIPNKIKFFLWRCAWDFLP 1005

Query: 444  TKVNLLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEI 503
                L  R ++ +P+C  C   AE  LH  W C   K +W  S +    + +    F E+
Sbjct: 1006 CGQILFNRKIAPTPICPNCHRKAESVLHAVWLCETAKEVWRNSAWGNVCEEWRVNSFREL 1065

Query: 504  IGAMKDNLTGLDFELVVIFWWSVWNLRNNLSWGGQPD----------------------G 563
              A++ + +G +  L     W +WN RN+  + G+ +                       
Sbjct: 1066 WHALQLSSSGEEQGLFAYLCWGLWNRRNSFIFEGKSETATQLLHRMTKLAQEFSNANNLS 1125

Query: 564  RDLWAYSSDYLSAFHE--------LKLNTDASVRPVQGEAGGGCVLRGADGEVFMTACL- 623
              +    S   +  H          K+N D +V+      G G V+R A+GE FM AC+ 
Sbjct: 1126 HTIHGRQSSPQAPLHGWRPPPAGIYKINVDGAVKSGDSVRGVGVVVRNANGE-FMAACVR 1185

Query: 624  SLQRCWSVDLAEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDD 658
             +Q  +     E  A   G++ A  + F   V+E D+   +  +          GLL+++
Sbjct: 1186 RIQASYGARQTELMATIEGLRFAIDMGFTAAVLEMDAQDCINSILSTEECNGIDGLLIEE 1245

BLAST of Lag0031509.1 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 416.8 bits (1070), Expect = 3.6e-112
Identity = 231/695 (33.24%), Postives = 358/695 (51.51%), Query Frame = 0

Query: 23   SLLCGAERRSQITGFRVDALAHRFRTYFWDDNLLFFRAIGSEALVIRELLVRYERALGQT 82
            +LL  AE++  I G      A      F DD+L+F RA   +   ++ L   Y +A GQ 
Sbjct: 453  NLLVQAEQQRHIHGIHFGKDAKVSHLLFADDSLIFTRAAVEDCKHLKALFECYGKASGQI 512

Query: 83   INYEKS------------VIALARIRGKRLNNISAKFFPCPAAHG--------------- 142
             N+EKS            + A++ I   ++ +   K+   P+  G               
Sbjct: 513  FNFEKSSMFFSKGTRNDQITAISEIFQLKVVSRHEKYLGLPSMIGRKTKSFFNEVKLRVL 572

Query: 143  -----WKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEE 202
                 W+ KFF+  GKEVL+K++ QAIP Y M+ F++P GL ++I + MARFWW   ++ 
Sbjct: 573  NKISSWQHKFFTSGGKEVLIKAVAQAIPTYAMSVFKIPLGLCEDIQKAMARFWWGTKQDR 632

Query: 203  RRIHWLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQS 262
            + IHW  W+ +   K  GG+GFR++  FNQAL+AKQ WR++Q PSSL+  VLK  YF  +
Sbjct: 633  KGIHWARWERISHSKARGGMGFRDLSSFNQALVAKQGWRIMQFPSSLVARVLKARYFKHT 692

Query: 263  GFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPV 322
            GF+ A +GS+PSFVW S++WGR++L +G RWRIGNG+   +YG+NW+P   + +  SAP 
Sbjct: 693  GFMNAGLGSKPSFVWRSIVWGRQVLHKGARWRIGNGQNVLVYGNNWIPRPTTFKPISAPS 752

Query: 323  LSPASTVSELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSV 382
            +   +TV+EL      W E L+   F   D EAI++IPL     ED+LIWH++K G +SV
Sbjct: 753  MGTDTTVAELIDEKQQWREDLILQHFRPEDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSV 812

Query: 383  KSGYRLAHTLATQDRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLPTKVNLLK 442
            KSGY++A  +   + P  SN +  +  W  +W+L +P K + FLWR  HD LPT  NL K
Sbjct: 813  KSGYQVAMRIKFPEDPSCSNHD--QNLWRFIWKLAIPEKVKIFLWRAAHDLLPTAENLWK 872

Query: 443  RGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEIIGAMKDN 502
            + +   P+C  C    E   H    C   + +W  S  A   +         ++      
Sbjct: 873  KKVLQEPMCQSCHCHVETVSHALVECNRARKIWRYSNLAEELRGVYRCDIVWMLQFWPRQ 932

Query: 503  LTGLDFELVVIFWWSVWNLRNNLSWGGQPDG-RDLWAYSSDYLSAFHEL----------- 562
               ++   V    W++W  RN   + G+ +    + A +   + +F ++           
Sbjct: 933  HAKVEGAEVAALLWAIWKARNKWLFEGKKENPLRVVANAEAIVESFKKIRQPEMVYKTKG 992

Query: 563  ----------------KLNTDASVRPVQGEAGGGCVLRGADGEVFMTACLSLQRCWSVDL 622
                            K+N DA+V      AG G V+R +DG     A  SL+   SV +
Sbjct: 993  NAERQKQWSPPPNGWQKVNVDAAVDVENQMAGLGVVVRDSDGNCRAAAIKSLRLPGSVAM 1052

Query: 623  AEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDDIRRILSPWDN 657
            AE  A+  G+++A +      + E+DSL ++ +++ +   ++E+G L+ DI+  L  + N
Sbjct: 1053 AEATAMEWGLKVAEKAHITFGIFESDSLEVIDLINKKSSSLTEIGWLISDIQENLQNFQN 1112

BLAST of Lag0031509.1 vs. NCBI nr
Match: XP_024956542.1 (uncharacterized protein LOC112498908 [Citrus sinensis])

HSP 1 Score: 402.1 bits (1032), Expect = 9.1e-108
Identity = 228/695 (32.81%), Postives = 346/695 (49.78%), Query Frame = 0

Query: 23   SLLCGAERRSQITGFRVDALAHRFRTYFWDDNLLFFRAIGSEALVIRELLVRYERALGQT 82
            SLL  AE++  I G             F DD+L+F RA  ++   ++ +L  Y  A GQ 
Sbjct: 424  SLLQQAEQQRLIHGLSFGKELKISHLLFADDSLIFCRASVADCQKLKMILDCYSLASGQI 483

Query: 83   INYEKSVIALA----RIRGKRLNNI--------SAKFFPCPAAHG--------------- 142
             N+EKS + L+    + +   + NI           +   PA  G               
Sbjct: 484  FNFEKSSLFLSGNVQQAQASAIGNIFHLNIVSKYENYLGLPAMVGRKRSGFFNDIKLKVL 543

Query: 143  -----WKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEE 202
                 W+ KFFS  GKEVL+K+ VQAIP + M+ F++P G+ ++I R +  FWW  S E 
Sbjct: 544  NKISSWQHKFFSCGGKEVLIKAAVQAIPAFAMSVFKIPLGICEDIQRIVGNFWWGSSRER 603

Query: 203  RRIHWLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQS 262
            R IHW  W+ +   KC GG+GFR+   FNQALLAKQ WR+ Q P SL+  VL+  YF  S
Sbjct: 604  RSIHWSKWEKMSQAKCKGGMGFRDFASFNQALLAKQGWRIQQCPDSLVARVLQARYFHSS 663

Query: 263  GFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPV 322
             FL A IGS PS++W S+LWGR+++  G RWRIGNG+   I+ +NW+P  F+ +    P 
Sbjct: 664  NFLNAKIGSNPSYIWRSILWGRQIICGGSRWRIGNGQDVHIHKANWIPKPFTFKPVIKPT 723

Query: 323  LSPASTVSELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSV 382
            +   + VSEL      W+E L+   F+  D + I +IPL     ED LIWHF K G ++V
Sbjct: 724  MPSEALVSELINGENCWDEELIYKHFDKMDADVITQIPLPRRLREDELIWHFGKSGQYTV 783

Query: 383  KSGYRLAHTLATQDRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLPTKVNLLK 442
            KSGY+ A  +     P SS S +    W+ +W L +P K R F+WR   + LP+  NL K
Sbjct: 784  KSGYQRALKIRFPAMPSSSESSKNE--WNIIWSLALPKKIRIFVWRAAKNLLPSAENLWK 843

Query: 443  RGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEIIGAMKDN 502
            R +   P C LC    E+  H    C   K +W  S F +  Q         ++  +K  
Sbjct: 844  RKIVQEPTCQLCKMGIENVFHALVDCKAAKKVWRLSLFYIDIQAAPGQDILSLLHGVKRM 903

Query: 503  LTGLDFELVVIFWWSVWNLRNN-LSWGGQPDGRDLWAYSSDYLSAFHE------------ 562
             +  D +L  +  W+ WN RN  L  G + + + + A +   + A+              
Sbjct: 904  RSNADVDLFAVMLWAKWNARNQWLFKGKRENPQSVVAKAEAVMEAYKRVQPSADVSHGKQ 963

Query: 563  ---------------LKLNTDASVRPVQGEAGGGCVLRGADGEVFMTACLSLQRCWSVDL 622
                           +K+NTDA+    +  AG G V+R  +G+V  TA    +   SV  
Sbjct: 964  QKVAQLGWNPPQEGFVKINTDAATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAY 1023

Query: 623  AEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDDIRRILSPWDN 657
            AE  A+  G+Q+A+     D ++E+DS  +V +++      SE+  ++ +I+++   +D+
Sbjct: 1024 AEAEAMEWGLQVAKDAHVKDVIMESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDH 1083

BLAST of Lag0031509.1 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.9e-48
Identity = 152/572 (26.57%), Postives = 249/572 (43.53%), Query Frame = 0

Query: 115 GWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIH 174
           GW+ K  S AG+  L K+++ ++P ++M+   LP+ ++  + +    F W  + E+++ H
Sbjct: 26  GWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLWGSTAEKKKQH 85

Query: 175 WLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLE 234
            + W  +C PK  GGLG R  +  N+AL++K  WR+LQ+ +SL   VL+  Y    G + 
Sbjct: 86  LVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQKKY--HVGEIR 145

Query: 235 ADIGSRP----SFVWGSLLWG-RELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAP 294
                 P    S  W S+  G R+++  G  W  G+G+    +   W+  +  L++ +  
Sbjct: 146 DSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKPLLELDNGE 205

Query: 295 VLSPASTV--SELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSG-EDRLIWHFEKHG 354
             +   TV   +L+    GW+ A +           +  + L   +G  DRL W F + G
Sbjct: 206 RPTDCDTVVAKDLWIPGRGWDFAKIDPYTTNNTRLELRAVVLDLVTGARDRLSWKFSQDG 265

Query: 355 NFSVKSGYRLAHTLATQDRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLPTKV 414
            FSV+S Y +  T+    RP  ++      +++ LW++ VP + + FLW + +  + T+ 
Sbjct: 266 QFSVRSAYEML-TVDEVPRPNMAS------FFNCLWKVRVPERVKTFLWLVGNQAVMTEE 325

Query: 415 NLLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQP-FSHLRFEEIIG 474
              +R LS S +C +C    E  LH+   CP    +W+        Q  FS   FE +  
Sbjct: 326 ERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQRRQQGFFSKSLFEWLYD 385

Query: 475 AMKDNLTGLDFE----LVVIFWWSVWNLRNNLSWGGQPDGRDL------W------AYSS 534
            + D     D        VI WW  W  R    +G     RD       W      A+S 
Sbjct: 386 NLGDRSGCEDIPWSTIFAVIIWWG-WKWRCGNIFGENTKCRDRVKFVKEWAVEVYRAHSG 445

Query: 535 DYLSAFHE----------------LKLNTDASVRPVQGEAGGGCVLRGADGEVFMTACLS 594
           + L    +                +K+NTD + R   G A  G VLR   G       L+
Sbjct: 446 NVLVGITQPRVERMIGWVSPCVGWVKVNTDGASRGNPGLASAGGVLRDCTGAWCGGFSLN 505

Query: 595 LQRCWSVDLAEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDDI 646
           + RC S   AE W VY G+  A +       +E DS  +V  L   + D   +  L+   
Sbjct: 506 IGRC-SAPQAELWGVYYGLYFAWEKKVPRVELEVDSEVIVGFLKTGISDSHPLSFLVRLC 565

BLAST of Lag0031509.1 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 4.8e-35
Identity = 67/149 (44.97%), Postives = 96/149 (64.43%), Query Frame = 0

Query: 136 AIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIHWLSWDSLCLPK-CLGGLGFRN 195
           A+P Y M+CFRL + L K++   M  FWW+  E +R+I W++W  LC  K   GGLGFR+
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 196 MELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLEADIGSRPSFVWGSLLWGREL 255
           +  FNQALLAKQ +R++  P +LL  +L+  YFP S  +E  +G+RPS+ W S++ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 256 LVRGCRWRIGNGRATPIYGSNWLPNEFSL 284
           L RG    IG+G  T ++   W+ +E  L
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPL 150

BLAST of Lag0031509.1 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 1.6e-134
Identity = 242/575 (42.09%), Postives = 336/575 (58.43%), Query Frame = 0

Query: 115  GWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIH 174
            GWK K FS+ GKEVL+K++ QAIPCYTM+CFRLP+ LI+E H   ARFWW  S+E+++IH
Sbjct: 571  GWKAKLFSIGGKEVLIKAVAQAIPCYTMSCFRLPKRLIREFHHITARFWWGSSKEDKKIH 630

Query: 175  WLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLE 234
            W++W+SL LPKC GG+GFR++ELFN+ALLAKQCWR+L  P+S+L  VLKG YF    F+E
Sbjct: 631  WVAWNSLYLPKCEGGMGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFME 690

Query: 235  ADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPVLSPA 294
            A I   PS++W S+LWGR+LL +G RWRIGNG +  IYG NW+PN+ +L+I S+P L   
Sbjct: 691  AKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLV 750

Query: 295  STVSELFT-ASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSVKSG 354
            S VS L     GGW   ++R  F   + + IL IP+  G+ EDRLIW++EK G +SV+SG
Sbjct: 751  SRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSG 810

Query: 355  YRLA-HTLATQDRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLPTKVNLLKRG 414
            Y++A         P SS+SE VR WW+G W++++PNK + FLWRLC DRLPT  NL KRG
Sbjct: 811  YKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRG 870

Query: 415  LSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEIIGAMKDNLT 474
            + ++  C  C  + ED +HLFW C   +++W+ SKF     PF  LR         ++L+
Sbjct: 871  VEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKL-SPFLILR------ESHESLS 930

Query: 475  GLDFELVVIFWWSVWNLRNNLSWGGQPD-----GRDLWAYSSDYLSAFHE---------- 534
              DFE + +  W +WN RN  ++          G +L  +++ Y   F E          
Sbjct: 931  KADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRV 990

Query: 535  ---------------LKLNTDASVRPVQGEAGGGCVLRGADGEVFMTACLSLQRCWSVDL 594
                            K+NTDAS       AG G ++    G+V   A   L+   SVD+
Sbjct: 991  TNTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDM 1050

Query: 595  AEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDDIRRILSPWDN 654
            AE  A   G+QLA ++                 +H  L D+SE G ++   +   +   +
Sbjct: 1051 AEAIAAVEGLQLASEIG----------------MHPALEDLSETGEIVLKAKNFWTQSLH 1110

Query: 655  GKVLFTPRQGNKVAHALARLAFSYAD-RVWLEEWP 657
                F  R+GNK AH LAR A    +  +W+E+WP
Sbjct: 1111 ASFNFVKREGNKAAHMLARRALLLHEFSIWMEDWP 1122

BLAST of Lag0031509.1 vs. ExPASy TrEMBL
Match: M5W5F3 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa026368mg PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 7.0e-122
Identity = 255/698 (36.53%), Postives = 367/698 (52.58%), Query Frame = 0

Query: 24   LLCGAERRSQITGFRVDALAHRF-RTYFWDDNLLFFRAIGSEALVIRELLVRYERALGQT 83
            LL GAERR  + G +V   A       F DD++LF +A   + + +  L   YE   GQ 
Sbjct: 357  LLRGAERRGDLVGVQVARGAPSVTHLLFADDSILFMKATNKDCMALETLFQTYEEVTGQQ 416

Query: 84   INYEKSVIALA------------------------------RIRGKRLNNISAKFFPCPA 143
            INY KS ++L+                               I GK    +         
Sbjct: 417  INYSKSALSLSPNATRADFDMIEGVLNVPVVRCHENYLGLPTIAGKGRKQLFQHLKDKLW 476

Query: 144  AH--GWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEE 203
             H  GWK K  S AGKE+L+K+++QAIP Y+M+CFR+P+GL KE++  MARFWW  ++++
Sbjct: 477  KHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFRIPKGLCKELNGIMARFWWAKAKDK 536

Query: 204  RRIHWLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQS 263
            R IHW+ W+ LC  K  GGLGFR++E FNQALLAKQCWR+L+ P SL+  + +  Y P  
Sbjct: 537  RGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRTPESLVARIFRARYHPSV 596

Query: 264  GFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPV 323
             FLEA++G+ PSF+W SL WG+ELL +G RWR+G+G +  +Y   WLP     +I S P 
Sbjct: 597  PFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGSGVSIQVYTDKWLPAPSCFKIMSPPQ 656

Query: 324  LSPASTVSELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSV 383
            L  ++ V +LFT+SG WN  LL+ IF   + +AIL+IPL   +G D LIWH+E++G +SV
Sbjct: 657  LPLSTRVCDLFTSSGQWNVPLLKDIFWDQEVDAILQIPLASLAGHDCLIWHYERNGMYSV 716

Query: 384  KSGYRLAHTLATQDRPGSSNSERVRM---WWSGLWRLNVPNKHRFFLWRLCHDRLPTKVN 443
            KSGYRLA     +D+     S RV +   +W  +W L +PNK +FFLWR   D LP    
Sbjct: 717  KSGYRLAG--LEKDKMSGEPSARVDLNSKFWKKIWALKIPNKIKFFLWRCAWDFLPCGQI 776

Query: 444  LLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEIIGAM 503
            L  R ++ +P+C  C   AE  LH  W C   K +W  S +    + +    F E+  A+
Sbjct: 777  LFNRKIAPTPICPKCHRKAESVLHAVWLCEAAKEVWRNSAWGNVCEVWRVNSFRELWHAL 836

Query: 504  KDNLTGLDFELVVIFWWSVWNLRNNLSWGGQPDG--------RDLWAYSSDYLSAFHELK 563
            + + +G +  L     W +WN RN+  + G+ +           L    SD  +  H + 
Sbjct: 837  QLSSSGEEQGLFAYLCWGLWNRRNSFIFEGKSETAIQLLSRMTKLAQEFSDANNILHTIH 896

Query: 564  LNTDASVRPVQG------------EAGGGCVLRGADGEVFMTACL-SLQRCWSVDLAEGW 623
                +   P+QG              G G V+R A+GE FM AC+  +   +     E  
Sbjct: 897  GRQSSPQAPLQGWRPPPAVKSGDSVRGVGVVVRNANGE-FMAACVRRIHASYGARQTELM 956

Query: 624  AVYRGIQLARQLAFIDFVVETDSLR-LVKILHGELHD------VSEVGLLMDDIRRILSP 657
            A   G++ A  + F D ++E D+   L  I   E ++      + EV  L+++ R ++  
Sbjct: 957  ATIEGLRFAIDMGFTDAILEMDAQDCLNSIFSTEEYNGIDGPLLEEVNYLLNNFRAVVCH 1016

BLAST of Lag0031509.1 vs. ExPASy TrEMBL
Match: A0A251NPF0 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G123900 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 7.0e-122
Identity = 255/698 (36.53%), Postives = 367/698 (52.58%), Query Frame = 0

Query: 24   LLCGAERRSQITGFRVDALAHRF-RTYFWDDNLLFFRAIGSEALVIRELLVRYERALGQT 83
            LL GAERR  + G +V   A       F DD++LF +A   + + +  L   YE   GQ 
Sbjct: 320  LLRGAERRGDLVGVQVARGAPSVTHLLFADDSILFMKATNKDCMALETLFQTYEEVTGQQ 379

Query: 84   INYEKSVIALA------------------------------RIRGKRLNNISAKFFPCPA 143
            INY KS ++L+                               I GK    +         
Sbjct: 380  INYSKSALSLSPNATRADFDMIEGVLNVPVVRCHENYLGLPTIAGKGRKQLFQHLKDKLW 439

Query: 144  AH--GWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEE 203
             H  GWK K  S AGKE+L+K+++QAIP Y+M+CFR+P+GL KE++  MARFWW  ++++
Sbjct: 440  KHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFRIPKGLCKELNGIMARFWWAKAKDK 499

Query: 204  RRIHWLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQS 263
            R IHW+ W+ LC  K  GGLGFR++E FNQALLAKQCWR+L+ P SL+  + +  Y P  
Sbjct: 500  RGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRTPESLVARIFRARYHPSV 559

Query: 264  GFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPV 323
             FLEA++G+ PSF+W SL WG+ELL +G RWR+G+G +  +Y   WLP     +I S P 
Sbjct: 560  PFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGSGVSIQVYTDKWLPAPSCFKIMSPPQ 619

Query: 324  LSPASTVSELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSV 383
            L  ++ V +LFT+SG WN  LL+ IF   + +AIL+IPL   +G D LIWH+E++G +SV
Sbjct: 620  LPLSTRVCDLFTSSGQWNVPLLKDIFWDQEVDAILQIPLASLAGHDCLIWHYERNGMYSV 679

Query: 384  KSGYRLAHTLATQDRPGSSNSERVRM---WWSGLWRLNVPNKHRFFLWRLCHDRLPTKVN 443
            KSGYRLA     +D+     S RV +   +W  +W L +PNK +FFLWR   D LP    
Sbjct: 680  KSGYRLAG--LEKDKMSGEPSARVDLNSKFWKKIWALKIPNKIKFFLWRCAWDFLPCGQI 739

Query: 444  LLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEIIGAM 503
            L  R ++ +P+C  C   AE  LH  W C   K +W  S +    + +    F E+  A+
Sbjct: 740  LFNRKIAPTPICPKCHRKAESVLHAVWLCEAAKEVWRNSAWGNVCEVWRVNSFRELWHAL 799

Query: 504  KDNLTGLDFELVVIFWWSVWNLRNNLSWGGQPDG--------RDLWAYSSDYLSAFHELK 563
            + + +G +  L     W +WN RN+  + G+ +           L    SD  +  H + 
Sbjct: 800  QLSSSGEEQGLFAYLCWGLWNRRNSFIFEGKSETAIQLLSRMTKLAQEFSDANNILHTIH 859

Query: 564  LNTDASVRPVQG------------EAGGGCVLRGADGEVFMTACL-SLQRCWSVDLAEGW 623
                +   P+QG              G G V+R A+GE FM AC+  +   +     E  
Sbjct: 860  GRQSSPQAPLQGWRPPPAVKSGDSVRGVGVVVRNANGE-FMAACVRRIHASYGARQTELM 919

Query: 624  AVYRGIQLARQLAFIDFVVETDSLR-LVKILHGELHD------VSEVGLLMDDIRRILSP 657
            A   G++ A  + F D ++E D+   L  I   E ++      + EV  L+++ R ++  
Sbjct: 920  ATIEGLRFAIDMGFTDAILEMDAQDCLNSIFSTEEYNGIDGPLLEEVNYLLNNFRAVVCH 979

BLAST of Lag0031509.1 vs. ExPASy TrEMBL
Match: A0A5E4FZN9 (PREDICTED: retrotransposon OS=Prunus dulcis OX=3755 GN=ALMOND_2B007697 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 2.0e-121
Identity = 254/706 (35.98%), Postives = 366/706 (51.84%), Query Frame = 0

Query: 24   LLCGAERRSQITGFRV----DALAHRFRTYFWDDNLLFFRAIGSEALVIRELLVRYERAL 83
            LL GAERR  + G +V     ++ H     F DD++LF +A       +  L   YE   
Sbjct: 586  LLRGAERRGDLVGVQVARGGPSVTH---LLFADDSILFMKATNEACRALETLFQTYEEVS 645

Query: 84   GQTINYEKSVIALA---------RIRGKRLN----NISAKFFPCPA-------------- 143
            GQ INY KS  +L+          I+G  LN        K+   P               
Sbjct: 646  GQQINYSKSAFSLSPNATRADFDMIKGV-LNVPVVQCHEKYLGLPTIAGKGRKQLFQHLK 705

Query: 144  ------AHGWKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNG 203
                    GWK K  S AGKE+L+K+++QAIP Y+M+CFR+P+GL KE++  MARFWW  
Sbjct: 706  DKLWKHISGWKEKLLSRAGKEILMKAVLQAIPTYSMSCFRIPKGLCKELNGIMARFWWAK 765

Query: 204  SEEERRIHWLSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCY 263
            ++++R IHW+ W+ LC  K  GGLGFR++E FNQALLAKQCWR+L+ P SL+  + +  Y
Sbjct: 766  AKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRTPESLVARIFRARY 825

Query: 264  FPQSGFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQ 323
             P   FLEA++G+ PSF+W SL WG+ELL +G RWR+GNG +  +Y   WLP     +I 
Sbjct: 826  HPSVPFLEAEVGTNPSFIWRSLQWGKELLNKGLRWRVGNGVSIQVYTDKWLPAPSFFKIM 885

Query: 324  SAPVLSPASTVSELFTASGGWNEALLRMIFNGADCEAILRIPLRHGSGEDRLIWHFEKHG 383
            S P L  ++ V +LFT+SG WN  LL+ IF   + +A L+IPL   +G D LIWH+E++G
Sbjct: 886  SPPQLPLSTLVCDLFTSSGQWNVPLLKDIFWDQEVDAKLQIPLASLAGHDCLIWHYERNG 945

Query: 384  NFSVKSGYRLAHTLATQDRPGSSNSERVRM---WWSGLWRLNVPNKHRFFLWRLCHDRLP 443
             +SVKSGYRLA     +D+     S RV +   +W  +W L +PNK +FFLWR   D LP
Sbjct: 946  MYSVKSGYRLA--CLEKDKMSGEPSVRVDLNSKFWKKIWALKIPNKIKFFLWRCAWDFLP 1005

Query: 444  TKVNLLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEEI 503
                L  R ++ +P+C  C   AE  LH  W C   K +W  S +    + +    F E+
Sbjct: 1006 CGQILFNRKIAPTPICPNCHRKAESVLHAVWLCETAKEVWRNSAWGNVCEEWRVNSFREL 1065

Query: 504  IGAMKDNLTGLDFELVVIFWWSVWNLRNNLSWGGQPD----------------------G 563
              A++ + +G +  L     W +WN RN+  + G+ +                       
Sbjct: 1066 WHALQLSSSGEEQGLFAYLCWGLWNRRNSFIFEGKSETATQLLHRMTKLAQEFSNANNLS 1125

Query: 564  RDLWAYSSDYLSAFHE--------LKLNTDASVRPVQGEAGGGCVLRGADGEVFMTACL- 623
              +    S   +  H          K+N D +V+      G G V+R A+GE FM AC+ 
Sbjct: 1126 HTIHGRQSSPQAPLHGWRPPPAGIYKINVDGAVKSGDSVRGVGVVVRNANGE-FMAACVR 1185

Query: 624  SLQRCWSVDLAEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDD 658
             +Q  +     E  A   G++ A  + F   V+E D+   +  +          GLL+++
Sbjct: 1186 RIQASYGARQTELMATIEGLRFAIDMGFTAAVLEMDAQDCINSILSTEECNGIDGLLIEE 1245

BLAST of Lag0031509.1 vs. ExPASy TrEMBL
Match: A0A2N9ESZ1 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS9799 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 1.8e-114
Identity = 249/732 (34.02%), Postives = 355/732 (48.50%), Query Frame = 0

Query: 3    FPPEIMLRLGFAQQWVDLIL--------SLLCGAERRSQITGFRVDALAHRFRTYFW--- 62
            F  ++M+++GF  QWV L++        SLL   E R  IT  R          Y +   
Sbjct: 961  FLRQVMIKMGFHSQWVSLLMECITTVSYSLLINGEPRGHITPSRGLRQGDPISPYLFLIC 1020

Query: 63   --DDNLLFFRAIGSEALVIRELLVRYERALGQTINYEKSVIALARIRGKRLNNI--SAKF 122
              DD+LLF RA  +E   ++++L  YE+A GQ +N  K+ +  +R   +       S   
Sbjct: 1021 AEDDSLLFCRATQTECQKLQDILQAYEKASGQQLNGAKTTLFFSRNTSQAAQEAIKSTLG 1080

Query: 123  FPCPAAH------------------------------GWKGKFFSVAGKEVLLKSIVQAI 182
             PC   +                              GWK K  S AG+E+L+K++VQAI
Sbjct: 1081 VPCIRQYEKYLGLLSLVGKEKMQCFSQIKERVWCKVKGWKEKLLSQAGREILIKAVVQAI 1140

Query: 183  PCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIHWLSWDSLCLPKCLGGLGFRNMEL 242
            P YTMNCF+LP  L K+I   + RFWW   E+ER+IHW+ W  LC  K +GGLGFR ++ 
Sbjct: 1141 PTYTMNCFKLPVKLCKDIEAIIRRFWWGQKEQERKIHWIKWKKLCQTKDMGGLGFRELQK 1200

Query: 243  FNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLEADIGSRPSFVWGSLLWGRELLVR 302
            FN ALLAKQ WR++    SL+  V    +FP    LEA   +R SF W S++  ++L++ 
Sbjct: 1201 FNIALLAKQFWRLMHCKGSLIYKVFSPKFFPTGNILEAQEKTRGSFAWRSIMQAKDLILA 1260

Query: 303  GCRWRIGNGRATPIYGSNWLPNEFSLQIQSAP-VLSPASTVSELFTAS-GGWNEALLRMI 362
            G  WR+GNG+  PI+ S WL  E   +I + P    P S+V+EL   +   W+   +R I
Sbjct: 1261 GSSWRVGNGQKIPIWNSKWLAEEGHRRIITPPSCYHPNSSVAELIQGTPPEWDVEKIRTI 1320

Query: 363  FNGADCEAILRIPLRHGSGEDRLIWHFEKHGNFSVKSGYRLAHTLATQDRPGSSNSERVR 422
            F   D +AIL+IPL   S  D+LIWH  K+G ++V+SGY +         PGSS   +  
Sbjct: 1321 FLPYDADAILQIPLSSRSPPDQLIWHATKNGKYTVRSGYHILLQEEQISNPGSSRLWKSD 1380

Query: 423  MWWSGLWRLNVPNKHRFFLWRLCHDRLPTKVNLLKRGLSVSPLCVLCDDDAEDCLHLFWA 482
              W  +W L VP K R FLWR CHD LP+K+ L++R + VSP C  C    EDCLH  W 
Sbjct: 1381 PLWKTIWSLRVPAKVRSFLWRACHDSLPSKLGLMRRQVGVSPWCDSCGSGIEDCLHALWN 1440

Query: 483  CPVVKSMWLGSKFALFYQPFSHLRFEEIIGAMKDNLTGLDFELVVIFWWSVWNLRN---- 542
            CP +  +W      +  Q   H  F E++  +      L  E   +  W +W+ RN    
Sbjct: 1441 CPALTPIWSSQHNLVDLQKIVHNSFAELVCNVGKLNHDLILETFAVTCWLIWHKRNQDRL 1500

Query: 543  -----NLSWGG---------------------QPDGRDLWAYSSDYLSAFHELKLNTDAS 602
                   S  G                      P  R LW        A H+ K+N D +
Sbjct: 1501 HLPSAEYSQIGPRAQALIQEFSSLHAQERRAKSPQPRILWR-----PPAMHQYKVNFDGA 1560

Query: 603  VRPVQGEAGGGCVLRGADGEVFMTACLSLQRCWSVDLAEGWAVYRGIQLARQLAFIDFVV 657
            +     + G G V+R   G V  T    +    S +L E  A  R IQ AR++  +D + 
Sbjct: 1561 IFKDAKKGGIGVVVRDKAGLVIATLSQRVMTSPSPELIEARAARRAIQFAREIGLLDVIF 1620

BLAST of Lag0031509.1 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 1.9e-63
Identity = 168/560 (30.00%), Postives = 256/560 (45.71%), Query Frame = 0

Query: 136 AIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIHWLSWDSLCLPKCLGGLGFRNM 195
           A+P YTM CF LP+ + K+I   +A FWW   +E + +HW +WD L   K  GG+GF+++
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 196 ELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLEADIGSRPSFVWGSLLWGRELL 255
           E FN ALL KQ WR+L  P SL+  V K  YF +S  L A +GSRPSFVW S+   +E+L
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 256 VRGCRWRIGNGRATPIYGSNWL---PNEFSLQIQSAPVLSPAST-----VSELFTASG-G 315
            +G R  +GNG    I+   WL   P   +L++Q  P    AS      VS+L   SG  
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGRE 181

Query: 316 WNEALLRMIFNGADCEAILRIPLRHGSGE--DRLIWHFEKHGNFSVKSGY-RLAHTLATQ 375
           W + ++ M+F   + E  L   LR G     D   W +   G+++VKSGY  L   +  +
Sbjct: 182 WRKDVIEMLF--PEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKR 241

Query: 376 DRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLPTKVNLLKRGLSVSPLCVLCD 435
             P   +   +   +  +W+     K + FLW+   + LP    L  R LS    C+ C 
Sbjct: 242 SSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCP 301

Query: 436 DDAEDCLHLFWACPVVKSMW--------LGSKFALFYQPFSHLRFEEIIGAMKDNLTGLD 495
              E   HL + C   +  W        LG ++A     + +L +   +G          
Sbjct: 302 SCKETVNHLLFKCTFARLTWAISSIPIPLGGEWA--DSIYVNLYWVFNLGNGNPQWEKAS 361

Query: 496 FELVVIFWWSVWNLRNNLSWGGQ-----------PDGRDLWAYSSDYLSA---------- 555
            +LV    W +W  RN L + G+            D  + W   ++  S           
Sbjct: 362 -QLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSS 421

Query: 556 --------FHELKLNTDASVRPVQGEAGGGCVLRGADGEVFMTACLSLQRCWSVDLAEGW 615
                      +K NTDA+        G G VLR   GEV      +L +  SV  AE  
Sbjct: 422 CGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELE 481

Query: 616 AVYRGIQLARQLAFIDFVVETDSLRLVKILHGELHDVSEVGLLMDDIRRILSPWDNGKVL 647
           A+   +    +  +   + E+DS  L++IL+ +      +   + D++R+LS +   K +
Sbjct: 482 AMRWAVLSLSRFQYNYVIFESDSQVLIEILNND-EIWPSLKPTIQDLQRLLSQFTEVKFV 541

BLAST of Lag0031509.1 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 151.0 bits (380), Expect = 3.4e-36
Identity = 67/149 (44.97%), Postives = 96/149 (64.43%), Query Frame = 0

Query: 136 AIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIHWLSWDSLCLPK-CLGGLGFRN 195
           A+P Y M+CFRL + L K++   M  FWW+  E +R+I W++W  LC  K   GGLGFR+
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 196 MELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLEADIGSRPSFVWGSLLWGREL 255
           +  FNQALLAKQ +R++  P +LL  +L+  YFP S  +E  +G+RPS+ W S++ GREL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 256 LVRGCRWRIGNGRATPIYGSNWLPNEFSL 284
           L RG    IG+G  T ++   W+ +E  L
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWIMDETPL 150

BLAST of Lag0031509.1 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 137.9 bits (346), Expect = 3.0e-32
Identity = 115/469 (24.52%), Postives = 192/469 (40.94%), Query Frame = 0

Query: 222 LKGCYFPQSGFLEADIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEF 281
           +K  YF     L+A +  + S+ W SLL G  LL +G R  IG+G+   I   N + +  
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHP 60

Query: 282 SLQIQSAPVLSPASTVSELFTASGG---WNEALLRMIFNGADCEAILRIPLRHGSGEDRL 341
              + +        T++ LF   G    W+++ +    + +D   I RI L      D++
Sbjct: 61  PRPLNTEETYK-EMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKI 120

Query: 342 IWHFEKHGNFSVKSGY-RLAHTLATQ----DRPGSSNSERVRMWWSGLWRLNVPNKHRFF 401
           IW++   G ++V+SGY  L H  +T     + P  S   + R     +W L +  K + F
Sbjct: 121 IWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTR-----IWNLPIMPKLKHF 180

Query: 402 LWRLCHDRLPTKVNLLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQ 461
           LWR     L T   L  RG+ + P C  C  + E   H  + CP     W  S  +L   
Sbjct: 181 LWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRN 240

Query: 462 PFSHLRFEEIIGAMKD---NLTGLDFELVVIFW--WSVWNLRNNLSWGGQPDGRDLWAYS 521
                 FEE I  + +   + T  DF  ++  W  W +W  RNN+ +    +       S
Sbjct: 241 QLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLS 300

Query: 522 S-----DYLSAFHE-------------------------LKLNTDASVRPVQGEAGGGCV 581
           +     D+L+A                            +K N DA     + EA GG +
Sbjct: 301 AKAETHDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWI 360

Query: 582 LRGADGEVFMTACLSLQRCWSVDLAEGWAVYRGIQLARQLAFIDFVVETDSLRLVKILHG 641
           +R   G       + L    +   AE  A+   +Q      +    +E D   L+ +++G
Sbjct: 361 IRNHYGTPISWGSMKLAHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLING 420

Query: 642 ELHDVSEVGLLMDDIRRILSPWDNGKVLFTPRQGNKVAHALARLAFSYA 648
            +   S +   ++DI    + + + +  F  R+GNK+AH LA+   +Y+
Sbjct: 421 -ISFHSSLANHLEDISFWANKFASIQFGFIRRKGNKLAHVLAKYGCTYS 462

BLAST of Lag0031509.1 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 99.8 bits (247), Expect = 9.0e-21
Identity = 85/331 (25.68%), Postives = 129/331 (38.97%), Query Frame = 0

Query: 116 WKGKFFSVAGKEVLLKSIVQAIPCYTMNCFRLPRGLIKEIHRTMARFWWNGSEEERRIHW 175
           W  +  S AG+  L+ S++ ++  + M+ FRLP   IKEI    + F W+G E   +   
Sbjct: 56  WTARHLSFAGRLQLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFLWSGPELNTKKAK 115

Query: 176 LSWDSLCLPKCLGGLGFRNMELFNQALLAKQCWRVLQDPSSLLGSVLKGCYFPQSGFLEA 235
           ++W  +C PK  GGLG R+++  N                       KG ++  SG    
Sbjct: 116 VAWSDVCTPKDEGGLGIRSLKEAN-----------------------KGSFWSISG--NT 175

Query: 236 DIGSRPSFVWGSLLWGRELLVRGCRWRIGNGRATPIYGSNWLPNEFSLQIQSAPVLSPAS 295
            +G   S++W  +L  R L     +  I NG  T  +  NW     S   +   V     
Sbjct: 176 TLG---SWMWKKILKHRALASGFVKHDIHNGSNTSFWFDNW-----SKIGRLIDVTGHRG 235

Query: 296 TVSELFTASGGWNEALLRMIFNGADCEAILRI-----PLRH---GSGEDRLIWHFEKHGN 355
            +    T      EA++         + +LRI      +RH    SGED + W     GN
Sbjct: 236 CIDMGITLHASVAEAVVNHRPRRHRHDTLLRIEDVIAEVRHQGLTSGEDTVRW----KGN 295

Query: 356 FSV-KSGYRLAHTLATQDRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLPTKV 415
             + K  +    T A    P    +     W+ G+W  +   K+    W    +RL T  
Sbjct: 296 GDIFKPCFNTKETWAATREPKLKVN-----WYKGVWFSHATPKYSVLAWIAIKNRLTTGD 344

Query: 416 NLLKRGLSVSPLCVLCDDDAEDCLHLFWACP 438
            +L         CVLC    E   HLF+ CP
Sbjct: 356 RMLSWNAGADSSCVLCHHLVETRDHLFFTCP 344

BLAST of Lag0031509.1 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 8.2e-14
Identity = 87/341 (25.51%), Postives = 136/341 (39.88%), Query Frame = 0

Query: 350 VKSGYRLA------HTLATQDRPGSSNSERVRMWWSGLWRLNVPNKHRFFLWRLCHDRLP 409
           ++SGY +A         A Q  PGS+  ++       +W+L+V  K + FLWR     L 
Sbjct: 6   LRSGYWVATHEDLLEEEAIQPPPGSTEVKQ------AIWKLHVAPKIKHFLWRCVTGALA 65

Query: 410 TKVNLLKRGLSVSPLCVLCDDDAEDCLHLFWACPVVKSMWLGSKFALFYQPFSHLRFEE- 469
           T   L  R +   P+C  C  + E   H+ + CP  +S+W  +   +  Q      FE+ 
Sbjct: 66  TNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQSVWRSANIIIGNQWGPPSSFEDN 125

Query: 470 ---IIGAMKDNLT-GLDFELVVIFWWSVWNLRNNLSWGGQPDGRDLWAY-----SSDYLS 529
              +I   K   T  LD  L     W +W  RN   +  +    D  A      ++++L+
Sbjct: 126 LNRLIQLSKTQTTNSLDRFLPFWIMWRLWKSRNVFLFQQKCQSPDYEARKGIQDATEWLN 185

Query: 530 AFHELKLNTDASV--RPVQ-----------------------GEAGG------GCVLRGA 589
           A +E   NT+  V   P+Q                       G   G      G  +R  
Sbjct: 186 A-NETTENTNVHVATNPIQTSRRDSSQWNPPPEGWVKCNFDSGYTQGSPYTRSGWTIREC 245

Query: 590 DGEVFMTACLSLQRCWSVDLAEGWAVYRGIQL--ARQLAFIDFVVETDSLRLVKIL-HGE 641
           +G + +     LQ       AE       +Q+  A  L ++ F  E+DS  LV ++ +GE
Sbjct: 246 NGHIVLCGNAKLQSSTCSLHAEALGFLHALQVIWAHGLRYVWF--ESDSKSLVTLINNGE 305