Lag0030964.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0030964.1 vs. NCBI nr
Match: XP_038878150.1 (uncharacterized protein LOC120070303 isoform X1 [Benincasa hispida] >XP_038878151.1 uncharacterized protein LOC120070303 isoform X1 [Benincasa hispida] >XP_038878152.1 uncharacterized protein LOC120070303 isoform X1 [Benincasa hispida])

HSP 1 Score: 630.2 bits (1624), Expect = 1.6e-176
Identity = 343/500 (68.60%), Postives = 368/500 (73.60%), Query Frame = 0

Query: 9   MQTNADSDCDISVLMINGVDLSSNLIGTEDEDFDSIEGASEEKEELQPNLGLERLKGEVL 68
           MQ NADSDCDIS                ED+DF S EGA EEKEELQ NL L+RLK    
Sbjct: 1   MQANADSDCDIS----------------EDDDFHSTEGAMEEKEELQLNLRLDRLK---- 60

Query: 69  SFLYPITRQCNLENDFYYFSSSYLLFTGGEQCAFEFSPASDAKRLNEDSPPGCASKRHRG 128
                                       G+QCA EFSPAS AKRLNEDSPPGCA+KR  G
Sbjct: 61  ----------------------------GKQCALEFSPASGAKRLNEDSPPGCATKRQTG 120

Query: 129 VCSWFPLNKEAKVVKVLNNSASCSSSHRTYSEACSSDKGPEAQPSYDQRRQKSHGYSFEL 188
           VCSWFP+NKE KVVKVLNNSAS SSS+  YS ACSSDK                      
Sbjct: 121 VCSWFPVNKETKVVKVLNNSASFSSSYIAYSGACSSDK---------------------- 180

Query: 189 PQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRTEKYPVAE 248
             GIRGKAKPKF FHSQRRE SCSVI EDRLCSA ISKV+NLSDTFDA  SRTEKYP AE
Sbjct: 181 --GIRGKAKPKFRFHSQRREQSCSVICEDRLCSASISKVYNLSDTFDAFASRTEKYPAAE 240

Query: 249 CIEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRPEWCRQVGGRVLPFA 308
           C+ED VEE+EDQS++GPA  CGN+E+SIAELLD LKDK+    G  EW  Q+GG++LP  
Sbjct: 241 CLEDLVEEFEDQSDIGPALNCGNSESSIAELLDVLKDKNSSLDGHLEWRSQLGGKMLPIV 300

Query: 309 ENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDRFEEALVAA 368
           E RALV   DR ADSE SH S D E SSDHEVNDQ LKLAG CT EQSITDRFEEALVAA
Sbjct: 301 EKRALVTSVDRRADSEDSHISSDNE-SSDHEVNDQNLKLAGLCTNEQSITDRFEEALVAA 360

Query: 369 CMDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCIDVKILSRSL 428
           CMDAE T GLVPNP+GIGLFGKLQ+VMQSEKERE+NFLNGLHC+T PNGCIDVKILSR L
Sbjct: 361 CMDAERTSGLVPNPLGIGLFGKLQQVMQSEKEREINFLNGLHCSTTPNGCIDVKILSRYL 420

Query: 429 DAKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVELEVGNLIRINPPWK 488
           DAKLTVCSCLFI+MERSLLQN DE++A E EKRTVIFS RVCS VELEVGNLIRINPPWK
Sbjct: 421 DAKLTVCSCLFINMERSLLQNGDESIATEGEKRTVIFSQRVCSVVELEVGNLIRINPPWK 427

Query: 489 EVPVDHVHNIILSTYFSQLT 509
           EVPVD  HNIILSTYF+QLT
Sbjct: 481 EVPVDRAHNIILSTYFAQLT 427

BLAST of Lag0030964.1 vs. NCBI nr
Match: XP_023525313.1 (uncharacterized protein LOC111788951 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.5 bits (1412), Expect = 6.0e-152
Identity = 271/334 (81.14%), Postives = 299/334 (89.52%), Query Frame = 0

Query: 175 DQRRQKSHGYSFELPQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF 234
           +++ +       E  +GIRGK KPKFTFHSQRRELSCSVI E+RLCSAPISKVHNLSDTF
Sbjct: 25  EEKEEVQLNLRLEKLKGIRGKTKPKFTFHSQRRELSCSVIHENRLCSAPISKVHNLSDTF 84

Query: 235 DAVISRTEKYPVAECIEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRP 294
           DAVISR+EKYPVA C+EDFVEE+EDQS+VGPAEKCGNTE+SI+ELLDGLKDK+ P G RP
Sbjct: 85  DAVISRSEKYPVAVCVEDFVEEFEDQSDVGPAEKCGNTESSISELLDGLKDKNDPLGCRP 144

Query: 295 EWCRQVGGRVLPFAENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKE 354
            W  QVGG+++P  E +ALVALGDR+ADSE SHTSVDTESSSDHEVNDQKLKLAGPCTKE
Sbjct: 145 GWRSQVGGKMIPIVEKKALVALGDRNADSEDSHTSVDTESSSDHEVNDQKLKLAGPCTKE 204

Query: 355 QSITDRFEEALVAACMDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTI 414
           QS+TDRFEEALVAACMDAE TL LVPNP+GIGL+GKLQRV+QSEKERE NFLNGLHC+T 
Sbjct: 205 QSMTDRFEEALVAACMDAERTLSLVPNPLGIGLYGKLQRVVQSEKERETNFLNGLHCSTT 264

Query: 415 PNGCIDVKILSRSLDAKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVE 474
           PNGCIDVK+LSR LDAKLTVCSCLFID+ERS LQN DEA+A E EKRTVIFSPRVCS+VE
Sbjct: 265 PNGCIDVKVLSRYLDAKLTVCSCLFIDVERSALQNGDEAIATEGEKRTVIFSPRVCSNVE 324

Query: 475 LEVGNLIRINPPWKEVPVDHVHNIILSTYFSQLT 509
           LEVGNLIRI PPWKEV VDH HNIILSTYFSQL+
Sbjct: 325 LEVGNLIRIIPPWKEVTVDHAHNIILSTYFSQLS 358

BLAST of Lag0030964.1 vs. NCBI nr
Match: XP_022948444.1 (uncharacterized protein LOC111452126 [Cucurbita moschata])

HSP 1 Score: 543.5 bits (1399), Expect = 1.9e-150
Identity = 269/334 (80.54%), Postives = 298/334 (89.22%), Query Frame = 0

Query: 175 DQRRQKSHGYSFELPQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF 234
           +++ +       E  +GIRGK KPKFTFHSQRRELSCSVI ++RLCSAPISKVHNLSDTF
Sbjct: 25  EEKEEVQLNLRLEKLKGIRGKTKPKFTFHSQRRELSCSVIHKNRLCSAPISKVHNLSDTF 84

Query: 235 DAVISRTEKYPVAECIEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRP 294
           DAVISR+EKYPVAEC+EDFVEE+EDQS+VGPAEKCGNTE+SI+ELLDGLKDK+ P G RP
Sbjct: 85  DAVISRSEKYPVAECVEDFVEEFEDQSDVGPAEKCGNTESSISELLDGLKDKNDPLGCRP 144

Query: 295 EWCRQVGGRVLPFAENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKE 354
            W  QVGG+++P  E +ALVALGDR+ADSE SHTSVDTESSSDHEVNDQKLKLAGPCTKE
Sbjct: 145 GWRSQVGGKMIPIIEKKALVALGDRNADSEDSHTSVDTESSSDHEVNDQKLKLAGPCTKE 204

Query: 355 QSITDRFEEALVAACMDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTI 414
           QSITDRFEEALVAACMDAE TL LVPNP+ IGL+GKLQRV+QSEKERE NFL+GLHC+T 
Sbjct: 205 QSITDRFEEALVAACMDAERTLSLVPNPLRIGLYGKLQRVVQSEKERETNFLSGLHCSTT 264

Query: 415 PNGCIDVKILSRSLDAKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVE 474
           PNGCIDVK+LSR LDAKLTVCSCLFID+E S LQN DEA+A E EKRTVIFSPRVCS+VE
Sbjct: 265 PNGCIDVKVLSRYLDAKLTVCSCLFIDVEHSALQNGDEAIATEGEKRTVIFSPRVCSNVE 324

Query: 475 LEVGNLIRINPPWKEVPVDHVHNIILSTYFSQLT 509
           LEVGNLIRI PPWKEV VDH HNIILSTYFSQL+
Sbjct: 325 LEVGNLIRIIPPWKEVSVDHAHNIILSTYFSQLS 358

BLAST of Lag0030964.1 vs. NCBI nr
Match: XP_022997742.1 (uncharacterized protein LOC111492608 isoform X3 [Cucurbita maxima])

HSP 1 Score: 536.2 bits (1380), Expect = 3.1e-148
Identity = 267/319 (83.70%), Postives = 289/319 (90.60%), Query Frame = 0

Query: 190 QGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRTEKYPVAEC 249
           +GIRGK KPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISR+EKYPVAEC
Sbjct: 38  KGIRGKEKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRSEKYPVAEC 97

Query: 250 IEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRPEWCRQVGGRVLPFAE 309
           +EDFVEE+EDQS+VGPAEK GNTE SI+ELLDGLKDK+ P G RP W  QVGG+++P  E
Sbjct: 98  VEDFVEEFEDQSDVGPAEKFGNTEISISELLDGLKDKNDPLGCRPGWRSQVGGKMIPIVE 157

Query: 310 NRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDRFEEALVAAC 369
            +ALVALGDR+ADSE S TSVD ESSSDH VN QKLKLAGPCTKEQSITDRFEEALVAAC
Sbjct: 158 KKALVALGDRNADSEDSRTSVDAESSSDHGVNAQKLKLAGPCTKEQSITDRFEEALVAAC 217

Query: 370 MDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCIDVKILSRSLD 429
           MDAE TL LVPNP+GIGL+GKLQRV+QSEKERE NFLNGLHC+T PNGCIDVK+LSR LD
Sbjct: 218 MDAERTLSLVPNPLGIGLYGKLQRVVQSEKERETNFLNGLHCSTTPNGCIDVKVLSRYLD 277

Query: 430 AKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVELEVGNLIRINPPWKE 489
           AKLTVCSCLFID+ER  L+N D+A+A E EKRTVIFSPRVCS+VELEVGNLIRI PPWKE
Sbjct: 278 AKLTVCSCLFIDVERLALRNGDKAIATEGEKRTVIFSPRVCSNVELEVGNLIRIIPPWKE 337

Query: 490 VPVDHVHNIILSTYFSQLT 509
           V VDH HNIILSTYFSQLT
Sbjct: 338 VTVDHAHNIILSTYFSQLT 356

BLAST of Lag0030964.1 vs. NCBI nr
Match: XP_022997741.1 (uncharacterized protein LOC111492608 isoform X2 [Cucurbita maxima])

HSP 1 Score: 536.2 bits (1380), Expect = 3.1e-148
Identity = 267/319 (83.70%), Postives = 289/319 (90.60%), Query Frame = 0

Query: 190 QGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRTEKYPVAEC 249
           +GIRGK KPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISR+EKYPVAEC
Sbjct: 40  KGIRGKEKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRSEKYPVAEC 99

Query: 250 IEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRPEWCRQVGGRVLPFAE 309
           +EDFVEE+EDQS+VGPAEK GNTE SI+ELLDGLKDK+ P G RP W  QVGG+++P  E
Sbjct: 100 VEDFVEEFEDQSDVGPAEKFGNTEISISELLDGLKDKNDPLGCRPGWRSQVGGKMIPIVE 159

Query: 310 NRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDRFEEALVAAC 369
            +ALVALGDR+ADSE S TSVD ESSSDH VN QKLKLAGPCTKEQSITDRFEEALVAAC
Sbjct: 160 KKALVALGDRNADSEDSRTSVDAESSSDHGVNAQKLKLAGPCTKEQSITDRFEEALVAAC 219

Query: 370 MDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCIDVKILSRSLD 429
           MDAE TL LVPNP+GIGL+GKLQRV+QSEKERE NFLNGLHC+T PNGCIDVK+LSR LD
Sbjct: 220 MDAERTLSLVPNPLGIGLYGKLQRVVQSEKERETNFLNGLHCSTTPNGCIDVKVLSRYLD 279

Query: 430 AKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVELEVGNLIRINPPWKE 489
           AKLTVCSCLFID+ER  L+N D+A+A E EKRTVIFSPRVCS+VELEVGNLIRI PPWKE
Sbjct: 280 AKLTVCSCLFIDVERLALRNGDKAIATEGEKRTVIFSPRVCSNVELEVGNLIRIIPPWKE 339

Query: 490 VPVDHVHNIILSTYFSQLT 509
           V VDH HNIILSTYFSQLT
Sbjct: 340 VTVDHAHNIILSTYFSQLT 358

BLAST of Lag0030964.1 vs. ExPASy TrEMBL
Match: A0A6J1G9X3 (uncharacterized protein LOC111452126 OS=Cucurbita moschata OX=3662 GN=LOC111452126 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 9.4e-151
Identity = 269/334 (80.54%), Postives = 298/334 (89.22%), Query Frame = 0

Query: 175 DQRRQKSHGYSFELPQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF 234
           +++ +       E  +GIRGK KPKFTFHSQRRELSCSVI ++RLCSAPISKVHNLSDTF
Sbjct: 25  EEKEEVQLNLRLEKLKGIRGKTKPKFTFHSQRRELSCSVIHKNRLCSAPISKVHNLSDTF 84

Query: 235 DAVISRTEKYPVAECIEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRP 294
           DAVISR+EKYPVAEC+EDFVEE+EDQS+VGPAEKCGNTE+SI+ELLDGLKDK+ P G RP
Sbjct: 85  DAVISRSEKYPVAECVEDFVEEFEDQSDVGPAEKCGNTESSISELLDGLKDKNDPLGCRP 144

Query: 295 EWCRQVGGRVLPFAENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKE 354
            W  QVGG+++P  E +ALVALGDR+ADSE SHTSVDTESSSDHEVNDQKLKLAGPCTKE
Sbjct: 145 GWRSQVGGKMIPIIEKKALVALGDRNADSEDSHTSVDTESSSDHEVNDQKLKLAGPCTKE 204

Query: 355 QSITDRFEEALVAACMDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTI 414
           QSITDRFEEALVAACMDAE TL LVPNP+ IGL+GKLQRV+QSEKERE NFL+GLHC+T 
Sbjct: 205 QSITDRFEEALVAACMDAERTLSLVPNPLRIGLYGKLQRVVQSEKERETNFLSGLHCSTT 264

Query: 415 PNGCIDVKILSRSLDAKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVE 474
           PNGCIDVK+LSR LDAKLTVCSCLFID+E S LQN DEA+A E EKRTVIFSPRVCS+VE
Sbjct: 265 PNGCIDVKVLSRYLDAKLTVCSCLFIDVEHSALQNGDEAIATEGEKRTVIFSPRVCSNVE 324

Query: 475 LEVGNLIRINPPWKEVPVDHVHNIILSTYFSQLT 509
           LEVGNLIRI PPWKEV VDH HNIILSTYFSQL+
Sbjct: 325 LEVGNLIRIIPPWKEVSVDHAHNIILSTYFSQLS 358

BLAST of Lag0030964.1 vs. ExPASy TrEMBL
Match: A0A6J1K5X7 (uncharacterized protein LOC111492608 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492608 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.5e-148
Identity = 267/319 (83.70%), Postives = 289/319 (90.60%), Query Frame = 0

Query: 190 QGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRTEKYPVAEC 249
           +GIRGK KPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISR+EKYPVAEC
Sbjct: 38  KGIRGKEKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRSEKYPVAEC 97

Query: 250 IEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRPEWCRQVGGRVLPFAE 309
           +EDFVEE+EDQS+VGPAEK GNTE SI+ELLDGLKDK+ P G RP W  QVGG+++P  E
Sbjct: 98  VEDFVEEFEDQSDVGPAEKFGNTEISISELLDGLKDKNDPLGCRPGWRSQVGGKMIPIVE 157

Query: 310 NRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDRFEEALVAAC 369
            +ALVALGDR+ADSE S TSVD ESSSDH VN QKLKLAGPCTKEQSITDRFEEALVAAC
Sbjct: 158 KKALVALGDRNADSEDSRTSVDAESSSDHGVNAQKLKLAGPCTKEQSITDRFEEALVAAC 217

Query: 370 MDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCIDVKILSRSLD 429
           MDAE TL LVPNP+GIGL+GKLQRV+QSEKERE NFLNGLHC+T PNGCIDVK+LSR LD
Sbjct: 218 MDAERTLSLVPNPLGIGLYGKLQRVVQSEKERETNFLNGLHCSTTPNGCIDVKVLSRYLD 277

Query: 430 AKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVELEVGNLIRINPPWKE 489
           AKLTVCSCLFID+ER  L+N D+A+A E EKRTVIFSPRVCS+VELEVGNLIRI PPWKE
Sbjct: 278 AKLTVCSCLFIDVERLALRNGDKAIATEGEKRTVIFSPRVCSNVELEVGNLIRIIPPWKE 337

Query: 490 VPVDHVHNIILSTYFSQLT 509
           V VDH HNIILSTYFSQLT
Sbjct: 338 VTVDHAHNIILSTYFSQLT 356

BLAST of Lag0030964.1 vs. ExPASy TrEMBL
Match: A0A6J1K8B6 (uncharacterized protein LOC111492608 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492608 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.5e-148
Identity = 267/319 (83.70%), Postives = 289/319 (90.60%), Query Frame = 0

Query: 190 QGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRTEKYPVAEC 249
           +GIRGK KPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISR+EKYPVAEC
Sbjct: 40  KGIRGKEKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRSEKYPVAEC 99

Query: 250 IEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRPEWCRQVGGRVLPFAE 309
           +EDFVEE+EDQS+VGPAEK GNTE SI+ELLDGLKDK+ P G RP W  QVGG+++P  E
Sbjct: 100 VEDFVEEFEDQSDVGPAEKFGNTEISISELLDGLKDKNDPLGCRPGWRSQVGGKMIPIVE 159

Query: 310 NRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDRFEEALVAAC 369
            +ALVALGDR+ADSE S TSVD ESSSDH VN QKLKLAGPCTKEQSITDRFEEALVAAC
Sbjct: 160 KKALVALGDRNADSEDSRTSVDAESSSDHGVNAQKLKLAGPCTKEQSITDRFEEALVAAC 219

Query: 370 MDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCIDVKILSRSLD 429
           MDAE TL LVPNP+GIGL+GKLQRV+QSEKERE NFLNGLHC+T PNGCIDVK+LSR LD
Sbjct: 220 MDAERTLSLVPNPLGIGLYGKLQRVVQSEKERETNFLNGLHCSTTPNGCIDVKVLSRYLD 279

Query: 430 AKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVELEVGNLIRINPPWKE 489
           AKLTVCSCLFID+ER  L+N D+A+A E EKRTVIFSPRVCS+VELEVGNLIRI PPWKE
Sbjct: 280 AKLTVCSCLFIDVERLALRNGDKAIATEGEKRTVIFSPRVCSNVELEVGNLIRIIPPWKE 339

Query: 490 VPVDHVHNIILSTYFSQLT 509
           V VDH HNIILSTYFSQLT
Sbjct: 340 VTVDHAHNIILSTYFSQLT 358

BLAST of Lag0030964.1 vs. ExPASy TrEMBL
Match: A0A6J1KAS4 (uncharacterized protein LOC111492608 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492608 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 2.6e-148
Identity = 268/334 (80.24%), Postives = 294/334 (88.02%), Query Frame = 0

Query: 175 DQRRQKSHGYSFELPQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF 234
           +++ +       E  +GIRGK KPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF
Sbjct: 25  EEKEEVQLNLRLEKLKGIRGKEKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF 84

Query: 235 DAVISRTEKYPVAECIEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRP 294
           DAVISR+EKYPVAEC+EDFVEE+EDQS+VGPAEK GNTE SI+ELLDGLKDK+ P G RP
Sbjct: 85  DAVISRSEKYPVAECVEDFVEEFEDQSDVGPAEKFGNTEISISELLDGLKDKNDPLGCRP 144

Query: 295 EWCRQVGGRVLPFAENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKE 354
            W  QVGG+++P  E +ALVALGDR+ADSE S TSVD ESSSDH VN QKLKLAGPCTKE
Sbjct: 145 GWRSQVGGKMIPIVEKKALVALGDRNADSEDSRTSVDAESSSDHGVNAQKLKLAGPCTKE 204

Query: 355 QSITDRFEEALVAACMDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTI 414
           QSITDRFEEALVAACMDAE TL LVPNP+GIGL+GKLQRV+QSEKERE NFLNGLHC+T 
Sbjct: 205 QSITDRFEEALVAACMDAERTLSLVPNPLGIGLYGKLQRVVQSEKERETNFLNGLHCSTT 264

Query: 415 PNGCIDVKILSRSLDAKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVE 474
           PNGCIDVK+LSR LDAKLTVCSCLFID+ER  L+N D+A+A E EKRTVIFSPRVCS+VE
Sbjct: 265 PNGCIDVKVLSRYLDAKLTVCSCLFIDVERLALRNGDKAIATEGEKRTVIFSPRVCSNVE 324

Query: 475 LEVGNLIRINPPWKEVPVDHVHNIILSTYFSQLT 509
           LEVGNLIRI PPWKEV VDH HNIILSTYFSQLT
Sbjct: 325 LEVGNLIRIIPPWKEVTVDHAHNIILSTYFSQLT 358

BLAST of Lag0030964.1 vs. ExPASy TrEMBL
Match: A0A6J1CQI7 (uncharacterized protein LOC111013241 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013241 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 3.7e-139
Identity = 253/319 (79.31%), Postives = 280/319 (87.77%), Query Frame = 0

Query: 190 QGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTFDAVISRTEKYPVAEC 249
           +GIRGKAKPKFTFHSQR+ELSC+VI +DRLC    SKVHNLSD  DA++SRTE +P AEC
Sbjct: 40  KGIRGKAKPKFTFHSQRKELSCTVIHDDRLCVP--SKVHNLSDNLDAILSRTENHPAAEC 99

Query: 250 IEDFVEEYEDQSEVGPAEKCGNTENSIAELLDGLKDKSGPGGGRPEWCRQVGGRVLPFAE 309
           +EDF+EE+E+  +VGPAE  GNTE+SIAELLDGL+DK+G  GGRP+ C QVGGR+ P  E
Sbjct: 100 LEDFLEEFEEHIDVGPAENFGNTESSIAELLDGLEDKNGLLGGRPKLCSQVGGRMHPIVE 159

Query: 310 NRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDRFEEALVAAC 369
             ALVALGDRS+DSE SHTSVDT SS   EVNDQKLKLA PCTKEQSITDRFEEALVAAC
Sbjct: 160 KGALVALGDRSSDSEDSHTSVDTGSSG--EVNDQKLKLAAPCTKEQSITDRFEEALVAAC 219

Query: 370 MDAEMTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCIDVKILSRSLD 429
           MDAE TLGLVPNP+GIGLFGKLQRVMQSEKERE+NFLNGLHC+T+PNGCIDVKILSR LD
Sbjct: 220 MDAERTLGLVPNPLGIGLFGKLQRVMQSEKEREVNFLNGLHCSTVPNGCIDVKILSRYLD 279

Query: 430 AKLTVCSCLFIDMERSLLQNDDEAMAAEAEKRTVIFSPRVCSHVELEVGNLIRINPPWKE 489
           AKLTVCSCLFIDME+S L N DE +A E EKRT+IFSPRVC +VELEVG+LIRINPPWKE
Sbjct: 280 AKLTVCSCLFIDMEQSTLHNGDEGIATEGEKRTIIFSPRVCRNVELEVGSLIRINPPWKE 339

Query: 490 VPVDHVHNIILSTYFSQLT 509
           VPVDH  NIILSTYFSQLT
Sbjct: 340 VPVDHAPNIILSTYFSQLT 354

BLAST of Lag0030964.1 vs. TAIR 10
Match: AT1G02960.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G02965.1); Has 100 Blast hits to 99 proteins in 48 species: Archae - 0; Bacteria - 4; Metazoa - 26; Fungi - 13; Plants - 29; Viruses - 0; Other Eukaryotes - 28 (source: NCBI BLink). )

HSP 1 Score: 144.1 bits (362), Expect = 3.2e-34
Identity = 123/393 (31.30%), Postives = 187/393 (47.58%), Query Frame = 0

Query: 129 VCSWFPLNKEAKVVKVLNNSASCSSSHRTYSEACSSDKGPEAQPSYDQRRQKSHGYSFEL 188
           V +W  ++KE K +  LN  AS +SSH +   A  S  G            K HG     
Sbjct: 119 VATWSAISKETKSLIHLNGVASVASSHLSGFRAKKSSNG-----------LKDHG----- 178

Query: 189 PQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF--DAVISRTEKYPV 248
                   +PKF+F+S                    SK+ ++++ F  D      E+ P+
Sbjct: 179 --------RPKFSFNSHTH-------------GETSSKISDMAEIFEPDVEDQAIEEDPI 238

Query: 249 AECIEDFVEEYEDQSEVGPAE------KCGNTENSIAELLDGLKDKSGPGGGRPEWCRQV 308
            EC   F E  E++  V  AE      +C  T++++ +L +   DK        E   + 
Sbjct: 239 IECPNSFDERSENRQGVSVAESREVLHEC--TKDAVPKLQEIPLDKIRLIKRSSELDSRH 298

Query: 309 GGRVLPFAENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDR 368
             +   F          D   D E     +D+ SSSD E + Q         K+Q + DR
Sbjct: 299 EAKSRKFTHKGNSSNFQDSDTDDE-LPGPMDSGSSSDDEPSYQSSVPNISNQKKQFVGDR 358

Query: 369 FEEALVAACMDAE-MTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHC---NTIPN 428
           F+EA+ A+ +  E +  G      G  L+GKLQ++M+ EKE EM  +  L         +
Sbjct: 359 FDEAIKASSLSKEGLLFGSPKLSGGSSLYGKLQQIMKQEKETEMEIMRKLQSGIGEADSS 418

Query: 429 GCIDVKILSRSLDAKLTVCSCLFIDME-RSLLQNDDEAMAAEAEKRTVIFSPRVCSHVEL 488
           G +DVK++SR L+ KL VC C  ID+   SLL  + +A+AA+  + T+IFSP+VC+ V++
Sbjct: 419 GYVDVKVMSRHLEGKLVVCKCSVIDLSGDSLLLKNTQALAAKETETTIIFSPKVCADVDI 471

Query: 489 EVGNLIRINPPWKEVPVDHVHN-IILSTYFSQL 508
           E+GN IR+  PWKE+ V   ++ IILS+YFS L
Sbjct: 479 EIGNFIRVYAPWKELEVQKTNDVIILSSYFSSL 471

BLAST of Lag0030964.1 vs. TAIR 10
Match: AT1G02960.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G02965.1); Has 83 Blast hits to 82 proteins in 37 species: Archae - 0; Bacteria - 2; Metazoa - 20; Fungi - 7; Plants - 29; Viruses - 0; Other Eukaryotes - 25 (source: NCBI BLink). )

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-31
Identity = 120/390 (30.77%), Postives = 182/390 (46.67%), Query Frame = 0

Query: 129 VCSWFPLNKEAKVVKVLNNSASCSSSHRTYSEACSSDKGPEAQPSYDQRRQKSHGYSFEL 188
           V +W  ++KE K +  LN  AS +SSH +   A  S  G            K HG     
Sbjct: 119 VATWSAISKETKSLIHLNGVASVASSHLSGFRAKKSSNG-----------LKDHG----- 178

Query: 189 PQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF--DAVISRTEKYPV 248
                   +PKF+F+S                    SK+ ++++ F  D      E+ P+
Sbjct: 179 --------RPKFSFNSHTH-------------GETSSKISDMAEIFEPDVEDQAIEEDPI 238

Query: 249 AECIEDFVEEYEDQSEVGPAE------KCGNTENSIAELLDGLKDKSGPGGGRPEWCRQV 308
            EC   F E  E++  V  AE      +C  T++++ +L +   DK        E   + 
Sbjct: 239 IECPNSFDERSENRQGVSVAESREVLHEC--TKDAVPKLQEIPLDKIRLIKRSSELDSRH 298

Query: 309 GGRVLPFAENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDR 368
             +   F          D   D E     +D+ SSSD E + Q         K+Q + DR
Sbjct: 299 EAKSRKFTHKGNSSNFQDSDTDDE-LPGPMDSGSSSDDEPSYQSSVPNISNQKKQFVGDR 358

Query: 369 FEEALVAACMDAE-MTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCI 428
           F+EA+ A+ +  E +  G      G  L+GKLQ++M+ EKE EM  +  L         I
Sbjct: 359 FDEAIKASSLSKEGLLFGSPKLSGGSSLYGKLQQIMKQEKETEMEIMRKLQSG------I 418

Query: 429 DVKILSRSLDAKLTVCSCLFIDME-RSLLQNDDEAMAAEAEKRTVIFSPRVCSHVELEVG 488
              ++SR L+ KL VC C  ID+   SLL  + +A+AA+  + T+IFSP+VC+ V++E+G
Sbjct: 419 GEAVMSRHLEGKLVVCKCSVIDLSGDSLLLKNTQALAAKETETTIIFSPKVCADVDIEIG 462

Query: 489 NLIRINPPWKEVPVDHVHN-IILSTYFSQL 508
           N IR+  PWKE+ V   ++ IILS+YFS L
Sbjct: 479 NFIRVYAPWKELEVQKTNDVIILSSYFSSL 462

BLAST of Lag0030964.1 vs. TAIR 10
Match: AT1G02960.3 (unknown protein; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G02965.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 76.3 bits (186), Expect = 8.2e-14
Identity = 88/323 (27.24%), Postives = 134/323 (41.49%), Query Frame = 0

Query: 129 VCSWFPLNKEAKVVKVLNNSASCSSSHRTYSEACSSDKGPEAQPSYDQRRQKSHGYSFEL 188
           V +W  ++KE K +  LN  AS +SSH +   A  S  G            K HG     
Sbjct: 119 VATWSAISKETKSLIHLNGVASVASSHLSGFRAKKSSNG-----------LKDHG----- 178

Query: 189 PQGIRGKAKPKFTFHSQRRELSCSVIREDRLCSAPISKVHNLSDTF--DAVISRTEKYPV 248
                   +PKF+F+S                    SK+ ++++ F  D      E+ P+
Sbjct: 179 --------RPKFSFNSHTH-------------GETSSKISDMAEIFEPDVEDQAIEEDPI 238

Query: 249 AECIEDFVEEYEDQSEVGPAE------KCGNTENSIAELLDGLKDKSGPGGGRPEWCRQV 308
            EC   F E  E++  V  AE      +C  T++++ +L +   DK        E   + 
Sbjct: 239 IECPNSFDERSENRQGVSVAESREVLHEC--TKDAVPKLQEIPLDKIRLIKRSSELDSRH 298

Query: 309 GGRVLPFAENRALVALGDRSADSEGSHTSVDTESSSDHEVNDQKLKLAGPCTKEQSITDR 368
             +   F          D   D E     +D+ SSSD E + Q         K+Q + DR
Sbjct: 299 EAKSRKFTHKGNSSNFQDSDTDDE-LPGPMDSGSSSDDEPSYQSSVPNISNQKKQFVGDR 358

Query: 369 FEEALVAACMDAE-MTLGLVPNPVGIGLFGKLQRVMQSEKEREMNFLNGLHCNTIPNGCI 428
           F+EA+ A+ +  E +  G      G  L+GKLQ++M+ EKE EM  +  L         I
Sbjct: 359 FDEAIKASSLSKEGLLFGSPKLSGGSSLYGKLQQIMKQEKETEMEIMRKLQSG------I 395

Query: 429 DVKILSRSLDAKLTVCSCLFIDM 443
              ++SR L+ KL VC C  ID+
Sbjct: 419 GEAVMSRHLEGKLVVCKCSVIDL 395