Lag0030882.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0030882.1 vs. NCBI nr
Match: XP_038893753.1 (kirola-like [Benincasa hispida])

HSP 1 Score: 201.8 bits (512), Expect = 4.1e-48
Identity = 102/151 (67.55%), Postives = 123/151 (81.46%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKSSGRRFYEFIK-NMDCL-PRMFPENFKGYEIVEGHGFTHGSVVLWK 60
           MAQIAKIS+QVQLKSSG +FYEF K NMD +  +MFPE ++  ++VEG+GF+HGSV+  K
Sbjct: 1   MAQIAKISKQVQLKSSGHKFYEFFKNNMDYIFVQMFPEIYQSGKVVEGNGFSHGSVIHLK 60

Query: 61  YDFGIPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDSNGSSVNWFVEF 120
           Y+ G P EVKER A+DDANKSI+ ECLEGDLFRDFE +K+ +QV +N SNGSSVNW VEF
Sbjct: 61  YNAGKPEEVKERIAIDDANKSITFECLEGDLFRDFELMKLKIQVFENGSNGSSVNWSVEF 120

Query: 121 VKANEDVAPPYNYLHMGVKASKGIDAYLCNN 150
           VKANEDVA P++YL    K SKG+D YLCNN
Sbjct: 121 VKANEDVATPHHYLLCLSKVSKGLDDYLCNN 151

BLAST of Lag0030882.1 vs. NCBI nr
Match: KAG6581204.1 (hypothetical protein SDJN03_21206, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017943.1 hypothetical protein SDJN02_19809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 197.2 bits (500), Expect = 1.0e-46
Identity = 91/150 (60.67%), Postives = 119/150 (79.33%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKSSGRRFYEFIKN-MDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKY 60
           MAQIAK+S++VQL+SSG +FYE +KN MD + +MFP+ +K ++++EG+G  HGS++  KY
Sbjct: 1   MAQIAKVSQKVQLRSSGHKFYELLKNKMDFVFQMFPDVYKSWKVLEGNGLAHGSIIHLKY 60

Query: 61  DFGIPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDSNGSSVNWFVEFV 120
           +   P E KER A+DDANKSI+ ECLEGDLFRDFE  K+ ++VV+N SNG S NW +E+V
Sbjct: 61  NVDGPSEAKERLAIDDANKSITFECLEGDLFRDFEVFKLKIEVVENGSNGCSTNWSIEYV 120

Query: 121 KANEDVAPPYNYLHMGVKASKGIDAYLCNN 150
           KANEDVAPP+NYL +  K SKGID YLC N
Sbjct: 121 KANEDVAPPHNYLIIAAKISKGIDDYLCKN 150

BLAST of Lag0030882.1 vs. NCBI nr
Match: KAG6581206.1 (Major latex protein 146, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 196.8 bits (499), Expect = 1.3e-46
Identity = 93/150 (62.00%), Postives = 120/150 (80.00%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKSSGRRFYEFIKN-MDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKY 60
           MAQIAK+S++VQL+SS  +FYEF+KN MD + +MFPE +K +++VEG+G+ HGSV+  KY
Sbjct: 1   MAQIAKVSQEVQLRSSAPKFYEFLKNKMDFVFQMFPEIYKSWKVVEGNGYAHGSVIHLKY 60

Query: 61  DFGIPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDSNGSSVNWFVEFV 120
           +   P EVKER ++DDAN+SI+ ECLEGDL RDFE +K+ ++VV+N SNGSS NW +EFV
Sbjct: 61  NVDGPSEVKERLSIDDANQSITFECLEGDLLRDFEVMKMKIEVVENGSNGSSANWSIEFV 120

Query: 121 KANEDVAPPYNYLHMGVKASKGIDAYLCNN 150
           KANEDVA P+NYL    K SKGID YLC N
Sbjct: 121 KANEDVATPHNYLLCVAKVSKGIDEYLCKN 150

BLAST of Lag0030882.1 vs. NCBI nr
Match: XP_023528799.1 (kirola-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 196.4 bits (498), Expect = 1.7e-46
Identity = 92/150 (61.33%), Postives = 118/150 (78.67%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKSSGRRFYEFIKN-MDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKY 60
           MAQIAK+S++VQL+SSG +FYE +KN MD + +MFPE +K ++++EG+G  HGS++  KY
Sbjct: 1   MAQIAKVSQKVQLRSSGHKFYELLKNKMDFVFQMFPEVYKSWKVLEGNGLAHGSIIYLKY 60

Query: 61  DFGIPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDSNGSSVNWFVEFV 120
           D     E KER A+DDANKSI+ ECLEGDLFRDFE  K+ ++VV+N SNG S NW +E+V
Sbjct: 61  DVDGLSEAKERLAIDDANKSITFECLEGDLFRDFEVFKLKIEVVENGSNGCSSNWSIEYV 120

Query: 121 KANEDVAPPYNYLHMGVKASKGIDAYLCNN 150
           KANEDVAPP+NYL +  K SKGID YLC N
Sbjct: 121 KANEDVAPPHNYLIIAAKISKGIDDYLCKN 150

BLAST of Lag0030882.1 vs. NCBI nr
Match: XP_022935319.1 (kirola-like [Cucurbita moschata])

HSP 1 Score: 195.7 bits (496), Expect = 3.0e-46
Identity = 90/150 (60.00%), Postives = 119/150 (79.33%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKSSGRRFYEFIKN-MDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKY 60
           MAQIAK+S++VQL+SSG +FYE +KN MD + +MFP+ +K ++++EG+G  HGS++  KY
Sbjct: 1   MAQIAKVSQKVQLRSSGHKFYELLKNKMDFVFQMFPDVYKSWKVLEGNGLAHGSIIHLKY 60

Query: 61  DFGIPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDSNGSSVNWFVEFV 120
           +   P + KER A+DDANKSI+ ECLEGDLFRDFE  K+ ++VV+N SNG S NW +E+V
Sbjct: 61  NVDGPSKAKERLAIDDANKSITFECLEGDLFRDFEVFKLKIEVVENGSNGCSTNWSIEYV 120

Query: 121 KANEDVAPPYNYLHMGVKASKGIDAYLCNN 150
           KANEDVAPP+NYL +  K SKGID YLC N
Sbjct: 121 KANEDVAPPHNYLIIAAKISKGIDDYLCKN 150

BLAST of Lag0030882.1 vs. ExPASy Swiss-Prot
Match: P19825 (Major latex protein 15 OS=Papaver somniferum OX=3469 GN=MLP15 PE=2 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 5.7e-16
Identity = 43/146 (29.45%), Postives = 77/146 (52.74%), Query Frame = 0

Query: 4   IAKISEQVQLKSSGRRFYEFIKNMDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKYDF-G 63
           + K+  + ++  +  ++Y+  K+ + LP   P  +   + VEGHG T G V  W Y   G
Sbjct: 11  VGKLITESEVNCNADKYYQIFKHHEDLPSAIPHIYTSVKAVEGHGTTSGCVKEWCYILEG 70

Query: 64  IPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDN-DSNGSSVNWFVEFVKA 123
            PL VKE+   +D  ++I+   +EG +  D+++    L V    +  GS V W V++ K 
Sbjct: 71  KPLTVKEKTTYNDETRTINHNGIEGGMMNDYKKFVATLVVKPKANGQGSIVTWIVDYEKI 130

Query: 124 NEDVAPPYNYLHMGVKASKGIDAYLC 148
           NED   P++YL    +  + ++++LC
Sbjct: 131 NEDSPVPFDYLAFFQQNIEDLNSHLC 156

BLAST of Lag0030882.1 vs. ExPASy Swiss-Prot
Match: Q41020 (Major latex protein 22 OS=Papaver somniferum OX=3469 GN=MLP22 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 9.7e-16
Identity = 45/145 (31.03%), Postives = 73/145 (50.34%), Query Frame = 0

Query: 4   IAKISEQVQLKSSGRRFYEFIKNMDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKYDF-G 63
           + K+  ++++  +   +Y+  K+ + LP   P  ++G + VEG   T G +  W Y   G
Sbjct: 12  VGKLVTELEVNCNADEYYKIFKHHEDLPNAIPHIYRGVKAVEGDRITSGFIKEWHYIIEG 71

Query: 64  IPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDSNGSSVNWFVEFVKAN 123
            PL  KER   +D  ++I    +EG L  D+++    L     D +GS V W VE+ K N
Sbjct: 72  KPLTCKERTTYEDEARTIHHSTVEGVLLDDYKKFDATLVNPKADGHGSIVTWIVEYEKIN 131

Query: 124 EDVAPPYNYLHMGVKASKGIDAYLC 148
           ED   P +YL    K  + ++ YLC
Sbjct: 132 EDSPVPISYLTFH-KIIEDLNTYLC 155

BLAST of Lag0030882.1 vs. ExPASy Swiss-Prot
Match: Q06394 (Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.2e-15
Identity = 44/147 (29.93%), Postives = 77/147 (52.38%), Query Frame = 0

Query: 4   IAKISEQVQLKSSGRRFYEFIKNMDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKYDF-G 63
           + K+  + ++  +  ++Y+  K+ + LP + P  +   + VEGHG T G V  W Y   G
Sbjct: 11  VGKLVMESEVNCNADKYYKLYKHHEDLPSVIPHIYTSVKAVEGHGTTSGCVKEWGYILEG 70

Query: 64  IPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDSNGSS--VNWFVEFVK 123
            PL  KE+   +D  ++I    + GDL  D+++    L VV+  SNG    V W +++ K
Sbjct: 71  KPLSCKEKTTYNDETRTIHHMVVAGDLMNDYKKFDATL-VVNPKSNGHGCIVKWTIDYEK 130

Query: 124 ANEDVAPPYNYLHMGVKASKGIDAYLC 148
            NED   P+ YL    + ++ + ++LC
Sbjct: 131 MNEDSPVPFGYLACYQQITEDLSSHLC 156

BLAST of Lag0030882.1 vs. ExPASy Swiss-Prot
Match: Q06395 (Major latex protein 149 OS=Papaver somniferum OX=3469 GN=MLP149 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.8e-15
Identity = 44/146 (30.14%), Postives = 76/146 (52.05%), Query Frame = 0

Query: 4   IAKISEQVQLKSSGRRFYEFIKNMDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKY-DFG 63
           + K+  + ++  +  ++Y+  K+ + LP   P      + VEGHG T G V  W Y   G
Sbjct: 11  VGKLVMETEVNCNADKYYQIYKHHEDLPSAIPHIVTSAKAVEGHGTTSGCVKEWGYMHEG 70

Query: 64  IPLEVKERRAVDDANKSISMECLEGDLFRDFEELKVNLQVVDNDS-NGSSVNWFVEFVKA 123
             L  KE+   +D  ++I     EGDL  D+++    L V   D+ +GS V + +++ K 
Sbjct: 71  KTLTCKEKTTYNDETRTICHSISEGDLMNDYKKFDATLVVDPKDNGHGSIVKYILDYEKI 130

Query: 124 NEDVAPPYNYLHMGVKASKGIDAYLC 148
           NED   P +YL +  +A++ ++ YLC
Sbjct: 131 NEDSPVPIHYLALCNQATEDLNTYLC 156

BLAST of Lag0030882.1 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.3e-14
Identity = 45/144 (31.25%), Postives = 75/144 (52.08%), Query Frame = 0

Query: 7   ISEQVQLKSSGRRFYE-FIKNMDCLPRMFPENFKGYEIVEGHGFTHGSVVLWKYDFGIPL 66
           +  +V++K+S  +F+  F      + +  P N +  ++ EG   T GS+V W Y      
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  EVKERR--AVDDANKSISMECLEGDLFRDFEELKVNLQVV-DNDSNGSSVNWFVEFVKAN 126
           +V + R  AVD     I+   +EGDL ++++   + +QV   +  +GS V+W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVAPPYNYLHMGVKASKGIDAYL 147
           E+VA P   L   V+ SK ID +L
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312