Lag0030805.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0030805.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	TPX2 domain-containing protein
Location	chr11: 1543973 .. 1545251 (-)
Sequence length	468
RNA-Seq Expression	Lag0030805.1
Synteny	Lag0030805.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAAGTCTCAACCCAAATCTTTGATCAAGGTAATTGTCACTCATTTTGATCTTAGCTTCTTGGTTTTGATGATATTGGTTTTTTTGAAAACCTTTCTTTTTCTTGATTTTAGGCGAAAGATGGAGTTAGAGATCGAGTTTTGGAGAAAACGGAGAAAGCTCGAGTAAGTTTTCGGGATTGGGATTCGAAATTGTTCTTTGTTTTCCCTTTGTTTTGTGGAATAATAATGGGAATTAGGTTTGAGTTTTGTTTTGTTTGCTTGTTTGTTTTCTTTCTAGGCTCCTCAAAAGGTTCTTGTGAAAGAGAACCCCAAGAAGCCGCCACAAGATTTCAAGTTGCATACGCAAGAAAGAGCAGTGAAACGGGCGATGTTTAACTATTCGGTGAGTCGAGTCTCTTGAGTTGTTTTAAAGCTGAAGCTTTTGAGTTCAGTGGTGATCATAACAGAAGAATTTCACGTTCAAATTCCTGTTGTATCGTTTTGTTTACGGTTCATATATTTTGATAGTCACTTGTTGGGTCTTGTGTTGATTTTTTAACGTAACCCACGAATATATATCAGGTAGCAACCAAGTTATATGTGACGGAGCTACAAAAGAAAGTGGAAGAGAAGTTGCACAAGGTAACAACTTTTGGCCAAATATATTTTCCATAACACACAAAACTTTGCAATAAAATAGTGCAGAAAAGAACTGAAAAAAAAAAACAAATTGTGAAACAGATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAGATGATTCCAAGAGCTCAGCTCATGCCCTACTTTGATAGACCCTATTTTCCACAAAGGTAGAGCAAAAAAAAAAAAAAAAAACGCTTTTATTTGCATTATTAAAAGCACTTTTACAAACGCTTTTATTTACATTTTTAAAAGCACTAAAGTTCATAAGACATTGTCAACAAGGAGCATAGCTCAACTGACATAAAGTCGTACGGATAACCATGAGATTTGTGGTTTGAATTTGTACTTGAAAAAAAAAAGTACTTTTACCAATAATACATCCTAAAAATATTTTTTAGAGGAAGTATTTAATTCATGCCGATATCAAAAGTGTTTATGAAAAAAAAAAAATGTATCACTCTCAAAAGTCATTCTAAACTCGCTTTGATTGTATTATAAGCAGATCGAATCGACCATTGACGATCCCGAGAGAGCCGAGTTTTTTGATGAACAAGGAGCAATGGAGTTGCAACAGTGACAGTGAACTTTACAGCTTTCAGAGACAAGCTCTGAAACCAATCAAATAA

mRNA sequence

ATGGACAAGTCTCAACCCAAATCTTTGATCAAGGCGAAAGATGGAGTTAGAGATCGAGTTTTGGAGAAAACGGAGAAAGCTCGAGCTCCTCAAAAGGTTCTTGTGAAAGAGAACCCCAAGAAGCCGCCACAAGATTTCAAGTTGCATACGCAAGAAAGAGCAGTGAAACGGGCGATGTTTAACTATTCGGTAGCAACCAAGTTATATGTGACGGAGCTACAAAAGAAAGTGGAAGAGAAGTTGCACAAGATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAGATGATTCCAAGAGCTCAGCTCATGCCCTACTTTGATAGACCCTATTTTCCACAAAGATCGAATCGACCATTGACGATCCCGAGAGAGCCGAGTTTTTTGATGAACAAGGAGCAATGGAGTTGCAACAGTGACAGTGAACTTTACAGCTTTCAGAGACAAGCTCTGAAACCAATCAAATAA

Coding sequence (CDS)

ATGGACAAGTCTCAACCCAAATCTTTGATCAAGGCGAAAGATGGAGTTAGAGATCGAGTTTTGGAGAAAACGGAGAAAGCTCGAGCTCCTCAAAAGGTTCTTGTGAAAGAGAACCCCAAGAAGCCGCCACAAGATTTCAAGTTGCATACGCAAGAAAGAGCAGTGAAACGGGCGATGTTTAACTATTCGGTAGCAACCAAGTTATATGTGACGGAGCTACAAAAGAAAGTGGAAGAGAAGTTGCACAAGATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAGATGATTCCAAGAGCTCAGCTCATGCCCTACTTTGATAGACCCTATTTTCCACAAAGATCGAATCGACCATTGACGATCCCGAGAGAGCCGAGTTTTTTGATGAACAAGGAGCAATGGAGTTGCAACAGTGACAGTGAACTTTACAGCTTTCAGAGACAAGCTCTGAAACCAATCAAATAA

Protein sequence

MDKSQPKSLIKAKDGVRDRVLEKTEKARAPQKVLVKENPKKPPQDFKLHTQERAVKRAMFNYSVATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWSCNSDSELYSFQRQALKPIK

Homology

[Close]

BLAST of Lag0030805.1 vs. NCBI nr
Match: KAG7036751.1 (Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 275.4 bits (703), Expect = 3.0e-70
Identity = 142/155 (91.61%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MDKSQPKSLIKAKDGVRDRVLEKTEKARAPQKVLVKENPKKPPQDFKLHTQERAVKRAMF 60
           MDKS PKS IKAKDGVRDRV EKTE+ R PQKV+VK+N KK PQDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKK-PQDFKLHTQERAVKRAMF 60

Query: 61  NYSVATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYS+ATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNSDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCN DSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 154

[Close]

BLAST of Lag0030805.1 vs. NCBI nr
Match: XP_023525926.1 (protein TPX2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 274.2 bits (700), Expect = 6.7e-70
Identity = 141/155 (90.97%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MDKSQPKSLIKAKDGVRDRVLEKTEKARAPQKVLVKENPKKPPQDFKLHTQERAVKRAMF 60
           MDKS PKS IKAKDGVRDRV +KTE+ R PQKV+VK+N KK PQDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFIKAKDGVRDRVSDKTERVRTPQKVVVKDNTKK-PQDFKLHTQERAVKRAMF 60

Query: 61  NYSVATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYS+ATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNSDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCN DSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 154

[Close]

BLAST of Lag0030805.1 vs. NCBI nr
Match: XP_022949252.1 (protein TPX2-like [Cucurbita moschata])

HSP 1 Score: 273.5 bits (698), Expect = 1.1e-69
Identity = 141/155 (90.97%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 1   MDKSQPKSLIKAKDGVRDRVLEKTEKARAPQKVLVKENPKKPPQDFKLHTQERAVKRAMF 60
           MDKS PKS IKAKDGVRDRV EKTE+ R PQKV+VKEN KK PQDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKENTKK-PQDFKLHTQERAVKRAMF 60

Query: 61  NYSVATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYS+ATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNSDSELYSFQRQALKPIK 156
           TIPREPSFL+NKEQWSCN DSE+YSFQRQ LKPIK
Sbjct: 121 TIPREPSFLINKEQWSCNGDSEVYSFQRQTLKPIK 154

[Close]

BLAST of Lag0030805.1 vs. NCBI nr
Match: XP_022998171.1 (protein TPX2 [Cucurbita maxima])

HSP 1 Score: 273.1 bits (697), Expect = 1.5e-69
Identity = 140/155 (90.32%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 1   MDKSQPKSLIKAKDGVRDRVLEKTEKARAPQKVLVKENPKKPPQDFKLHTQERAVKRAMF 60
           MDKS PKS IKAKDG+RDRV +KTE+ R PQKV+VKEN KK PQDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFIKAKDGIRDRVSDKTERVRTPQKVVVKENTKK-PQDFKLHTQERAVKRAMF 60

Query: 61  NYSVATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYS+ATKLYV ELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVMELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNSDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCN+DSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNTDSEVYSFQRQALKPIK 154

[Close]

BLAST of Lag0030805.1 vs. NCBI nr
Match: XP_004145652.1 (protein TPX2 [Cucumis sativus])

HSP 1 Score: 270.4 bits (690), Expect = 9.7e-69
Identity = 143/156 (91.67%), Postives = 147/156 (94.23%), Query Frame = 0


Query: 1   MDKSQPKSLI-KAKDGVRDRVLEKTEKARAPQKVLVKENPKKPPQDFKLHTQERAVKRAM 60
           MDKSQPKSL+ K KDGVRDRVLEK E+AR PQK  VKEN KK  QDFKLHTQERAVKRAM
Sbjct: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKK-SQDFKLHTQERAVKRAM 60

Query: 61  FNYSVATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120
           FNYS+ATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP
Sbjct: 61  FNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120

Query: 121 LTIPREPSFLMNKEQWSCNSDSELYSFQRQALKPIK 156
           LTIPREPSFLMNKEQWSCN+DSELYSFQRQALKPIK
Sbjct: 121 LTIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 155

[Close]

BLAST of Lag0030805.1 vs. ExPASy Swiss-Prot
Match: F4I2H7 (Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.4e-08
Identity = 41/127 (32.28%), Postives = 71/127 (55.91%), Query Frame = 0


Query: 7   KSLIKAKDGVRDRVLEKTEKARAPQKVLVKENPKKPPQDFKLHTQERAVKRAMFNYSVAT 66
           K L KA+  +++  +   EK R P   +         Q+F LH + RAV+RA F++ +  
Sbjct: 617 KRLFKAQPVIKEDPIPVPEKVRMPLTEI---------QEFNLHVEHRAVERADFDHKIKE 676

Query: 67  KLYVTELQKKVEEKLHKMIEEEE-VRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPRE 126
           K    +  ++ E +  KM+EEE  ++ MRK M+P A+ +P F++P+ PQ+SN+  T  + 
Sbjct: 677 KENQYKRYRE-ESEAAKMVEEERALKQMRKTMVPHARPVPNFNKPFLPQKSNKGTTKAKS 733

Query: 127 PSFLMNK 133
           P+  + K
Sbjct: 737 PNLRVIK 733

The following BLAST results are available for this feature:

BLAST of Lag0030805.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036751.1	3.0e-70	91.61	Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023525926.1	6.7e-70	90.97	protein TPX2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949252.1	1.1e-69	90.97	protein TPX2-like [Cucurbita moschata]	[more]
XP_022998171.1	1.5e-69	90.32	protein TPX2 [Cucurbita maxima]	[more]
XP_004145652.1	9.7e-69	91.67	protein TPX2 [Cucumis sativus]	[more]

BLAST of Lag0030805.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I2H7	4.4e-08	32.28	Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 70..90
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR14326:SF39	TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILY	coord: 4..147
IPR027329	TPX2, C-terminal	PFAM	PF06886	TPX2	coord: 46..127 e-value: 2.6E-20 score: 72.4
IPR009675	TPX2	PANTHER	PTHR14326	TARGETING PROTEIN FOR XKLP2	coord: 4..147

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0030805	Lag0030805	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0030805.1.exon6	Lag0030805.1.exon6	exon
Lag0030805.1.exon5	Lag0030805.1.exon5	exon
Lag0030805.1.exon4	Lag0030805.1.exon4	exon
Lag0030805.1.exon3	Lag0030805.1.exon3	exon
Lag0030805.1.exon2	Lag0030805.1.exon2	exon
Lag0030805.1.exon1	Lag0030805.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0030805.1	cds.Lag0030805.1_6	CDS
cds.Lag0030805.1	cds.Lag0030805.1_5	CDS
cds.Lag0030805.1	cds.Lag0030805.1_4	CDS
cds.Lag0030805.1	cds.Lag0030805.1_3	CDS
cds.Lag0030805.1	cds.Lag0030805.1_2	CDS
cds.Lag0030805.1	cds.Lag0030805.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0030805.1	Lag0030805.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032147	activation of protein kinase activity
biological_process	GO:0060236	regulation of mitotic spindle organization
biological_process	GO:0090307	mitotic spindle assembly
cellular_component	GO:0005874	microtubule
cellular_component	GO:0005819	spindle
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0072686	mitotic spindle
cellular_component	GO:0005880	nuclear microtubule
molecular_function	GO:0008017	microtubule binding
molecular_function	GO:0030295	protein kinase activator activity